AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0832770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0832770 MtrunA17_Chr5g0397991 99.072 539 3 2 1 537 1 539 0.0 1074
Msa0832770 MtrunA17_Chr1g0203791 67.351 536 155 5 1 521 1 531 0.0 704
Msa0832770 MtrunA17_Chr6g0473701 65.769 520 157 4 12 526 89 592 0.0 673
Msa0832770 MtrunA17_Chr5g0428251 57.722 518 210 2 1 518 1 509 0.0 610
Msa0832770 MtrunA17_Chr3g0140931 56.015 532 207 5 32 536 13 544 0.0 589
Msa0832770 MtrunA17_Chr8g0389801 54.167 456 207 1 51 506 10 463 0.0 522
Msa0832770 MtrunA17_Chr4g0013031 52.371 464 218 2 64 524 66 529 2.81e-169 489
Msa0832770 MtrunA17_Chr6g0467321 46.124 516 252 7 18 518 4 508 7.02e-161 468
Msa0832770 MtrunA17_Chr2g0295631 43.305 478 251 4 63 521 19 495 1.28e-132 395
Msa0832770 MtrunA17_Chr6g0487091 52.744 328 146 2 192 519 87 405 2.85e-120 360
Msa0832770 MtrunA17_Chr6g0487091 48.101 79 41 0 47 125 10 88 8.86e-17 82.8
Msa0832770 MtrunA17_Chr6g0483381 35.220 477 301 3 42 513 5 478 1.09e-98 307
Msa0832770 MtrunA17_Chr8g0392601 36.504 452 271 7 61 504 9 452 7.78e-88 279
Msa0832770 MtrunA17_Chr6g0483361 33.626 455 300 1 52 504 13 467 3.28e-87 277
Msa0832770 MtrunA17_Chr6g0477961 33.543 477 309 3 42 513 5 478 3.62e-87 277
Msa0832770 MtrunA17_Chr6g0483341 33.407 455 294 3 64 513 41 491 4.95e-86 275
Msa0832770 MtrunA17_Chr1g0208791 33.056 481 305 6 47 519 27 498 2.15e-84 270
Msa0832770 MtrunA17_Chr1g0208821 33.114 456 299 2 52 504 29 481 4.80e-84 269
Msa0832770 MtrunA17_Chr6g0449061 32.568 479 302 8 34 504 6 471 2.09e-77 252
Msa0832770 MtrunA17_Chr2g0317131 32.860 493 307 5 25 514 16 487 1.21e-76 250
Msa0832770 MtrunA17_Chr6g0483331 31.448 442 302 1 64 504 12 453 7.49e-76 247
Msa0832770 MtrunA17_Chr7g0251331 31.568 472 308 6 53 515 16 481 3.18e-74 243
Msa0832770 MtrunA17_Chr6g0483351 34.211 380 248 1 123 500 1 380 3.47e-72 236
Msa0832770 MtrunA17_Chr2g0317161 32.696 471 299 5 61 527 28 484 4.47e-72 238
Msa0832770 MtrunA17_Chr2g0322671 29.505 444 312 1 72 514 60 503 4.71e-71 236
Msa0832770 MtrunA17_Chr1g0208891 33.624 458 294 4 63 515 23 475 3.86e-70 232
Msa0832770 MtrunA17_Chr2g0317151 34.312 443 268 5 61 492 30 460 2.97e-68 228
Msa0832770 MtrunA17_Chr4g0024501 28.444 450 321 1 65 513 65 514 1.25e-67 227
Msa0832770 MtrunA17_Chr8g0368001 28.932 515 331 10 18 516 16 511 2.46e-67 226
Msa0832770 MtrunA17_Chr2g0317171 31.992 472 301 7 61 527 34 490 9.29e-67 224
Msa0832770 MtrunA17_Chr1g0208881 32.075 477 312 6 40 510 1 471 1.38e-66 223
Msa0832770 MtrunA17_Chr6g0483161 34.218 377 229 2 122 498 2 359 4.86e-66 218
Msa0832770 MtrunA17_Chr2g0317221 32.683 410 258 5 122 527 9 404 3.04e-58 199
Msa0832770 MtrunA17_Chr6g0468521 28.189 486 336 4 40 517 1 481 8.09e-58 200
Msa0832770 MtrunA17_Chr5g0412541 28.855 454 319 3 65 516 37 488 1.94e-57 199
Msa0832770 MtrunA17_Chr2g0288281 29.288 379 268 0 126 504 4 382 7.87e-57 194
Msa0832770 MtrunA17_Chr1g0202761 28.958 480 321 7 51 526 34 497 1.96e-55 194
Msa0832770 MtrunA17_Chr7g0254521 28.105 459 321 3 64 518 31 484 8.38e-55 192
Msa0832770 MtrunA17_Chr5g0412771 26.523 509 348 7 18 519 21 510 4.60e-54 191
Msa0832770 MtrunA17_Chr6g0468531 29.363 487 330 5 40 517 1 482 2.62e-53 187
Msa0832770 MtrunA17_Chr5g0442331 31.222 442 292 5 87 523 69 503 3.47e-53 188
Msa0832770 MtrunA17_Chr1g0154441 29.474 380 263 3 127 504 5 381 1.33e-52 183
Msa0832770 MtrunA17_Chr6g0468511 28.511 470 323 4 40 501 1 465 1.56e-52 186
Msa0832770 MtrunA17_Chr7g0251321 31.000 400 243 7 123 515 1 374 2.17e-52 182
Msa0832770 MtrunA17_Chr7g0254511 26.696 457 325 4 64 516 30 480 2.43e-51 182
Msa0832770 MtrunA17_Chr3g0132531 25.646 503 355 7 31 520 6 502 2.86e-49 177
Msa0832770 MtrunA17_Chr5g0412571 30.075 399 272 6 122 516 39 434 6.33e-47 169
Msa0832770 MtrunA17_Chr7g0248051 27.517 447 322 1 65 511 71 515 3.18e-45 166
Msa0832770 MtrunA17_Chr7g0254531 27.342 395 286 1 123 517 1 394 5.09e-43 158
Msa0832770 MtrunA17_Chr6g0460541 25.553 407 283 2 122 519 4 399 3.62e-41 153
Msa0832770 MtrunA17_Chr5g0398571 31.544 298 197 4 220 513 2 296 6.95e-41 150
Msa0832770 MtrunA17_Chr3g0132541 22.131 488 317 6 34 514 4 435 7.34e-34 133
Msa0832770 MtrunA17_Chr6g0483264 36.139 202 125 2 303 502 1 200 1.02e-31 122
Msa0832770 MtrunA17_Chr8g0364761 33.468 248 158 5 87 330 76 320 6.97e-31 122
Msa0832770 MtrunA17_Chr7g0254501 24.281 313 235 2 207 519 1 311 5.58e-30 119
Msa0832770 MtrunA17_Chr5g0440661 27.324 355 193 8 123 476 1 291 7.44e-25 105
Msa0832770 MtrunA17_Chr6g0483241 32.778 180 121 0 122 301 2 181 1.22e-24 100
Msa0832770 MtrunA17_Chr6g0483261 37.419 155 93 2 350 502 6 158 1.10e-22 95.5
Msa0832770 MtrunA17_Chr6g0483221 36.913 149 94 0 350 498 6 154 2.69e-22 94.4
Msa0832770 MtrunA17_Chr6g0460531 30.932 236 156 5 53 285 13 244 2.14e-21 94.0
Msa0832770 MtrunA17_Chr8g0364751 31.138 167 111 2 350 514 19 183 1.79e-20 89.4
Msa0832770 MtrunA17_Chr5g0398551 52.381 84 40 0 60 143 39 122 2.35e-20 87.4
Msa0832770 MtrunA17_Chr5g0442311 31.395 172 118 0 126 297 4 175 2.50e-15 74.3
Msa0832770 MtrunA17_Chr6g0460521 24.537 216 163 0 303 518 4 219 3.49e-14 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0832770 AT4G23030.1 69.008 484 143 3 40 516 1 484 0.0 616
Msa0832770 AT1G58340.1 62.115 454 172 0 54 507 47 500 0.0 558
Msa0832770 AT4G29140.1 56.118 474 204 2 62 535 53 522 0.0 553
Msa0832770 AT5G52050.1 55.556 495 208 4 34 518 5 497 0.0 546
Msa0832770 AT5G19700.1 53.673 490 216 1 23 512 5 483 0.0 543
Msa0832770 AT2G38510.1 50.000 458 228 1 64 520 5 462 5.52e-169 488
Msa0832770 AT1G71870.1 46.531 490 244 3 47 518 6 495 3.22e-156 456
Msa0832770 AT5G49130.1 46.004 463 232 3 63 509 24 484 9.46e-134 398
Msa0832770 AT4G22790.1 41.064 470 263 10 56 518 21 483 8.07e-93 292
Msa0832770 AT1G71140.1 33.839 461 292 6 54 509 18 470 3.91e-82 264
Msa0832770 AT5G52450.1 33.671 493 300 7 39 522 11 485 1.28e-81 263
Msa0832770 AT2G04070.1 34.316 475 306 4 40 510 1 473 2.24e-81 262
Msa0832770 AT2G34360.1 34.221 488 289 10 37 514 5 470 4.37e-81 261
Msa0832770 AT1G73700.1 34.440 482 304 4 39 516 3 476 5.35e-81 261
Msa0832770 AT2G04080.1 33.049 469 307 3 40 504 1 466 5.59e-80 258
Msa0832770 AT2G04050.1 34.183 471 297 4 44 510 12 473 6.75e-78 253
Msa0832770 AT2G04080.2 32.688 465 306 3 40 500 1 462 1.59e-76 249
Msa0832770 AT1G15150.1 31.579 456 308 2 65 518 31 484 1.02e-75 248
Msa0832770 AT2G04040.1 32.280 443 294 2 65 504 27 466 2.48e-75 246
Msa0832770 AT1G15170.1 34.157 445 283 4 65 504 34 473 8.39e-75 245
Msa0832770 AT1G15160.1 31.799 478 318 3 47 518 9 484 1.49e-74 244
Msa0832770 AT2G04090.1 32.731 443 292 3 65 504 30 469 6.60e-73 240
Msa0832770 AT2G04100.1 32.731 443 292 3 65 504 30 469 1.34e-72 239
Msa0832770 AT1G66760.2 32.285 477 305 7 36 504 1 467 4.45e-72 238
Msa0832770 AT1G64820.1 31.590 478 306 6 61 523 25 496 4.51e-72 238
Msa0832770 AT1G61890.1 28.628 503 347 6 18 516 2 496 2.96e-71 236
Msa0832770 AT3G23550.1 33.040 454 293 3 47 497 18 463 1.19e-70 234
Msa0832770 AT2G04100.2 32.494 437 289 2 65 498 30 463 5.70e-70 232
Msa0832770 AT1G66760.1 32.059 471 302 7 36 498 1 461 1.16e-69 231
Msa0832770 AT1G66780.1 31.878 458 302 4 52 504 22 474 2.08e-68 228
Msa0832770 AT2G34360.2 31.340 485 265 8 37 514 5 428 2.30e-68 227
Msa0832770 AT1G15180.1 32.008 478 315 4 31 504 3 474 3.59e-68 228
Msa0832770 AT1G11670.1 29.213 445 311 2 62 504 47 489 3.95e-68 228
Msa0832770 AT1G61890.2 28.454 485 337 5 18 498 2 480 1.46e-67 226
Msa0832770 AT3G23560.1 34.845 419 263 5 63 476 37 450 2.73e-67 225
Msa0832770 AT3G21690.1 26.866 469 339 1 47 515 38 502 3.13e-64 218
Msa0832770 AT4G21910.3 27.701 509 342 5 24 513 2 503 1.02e-63 216
Msa0832770 AT4G21910.1 27.701 509 342 5 24 513 2 503 1.02e-63 216
Msa0832770 AT4G21910.2 29.111 450 317 2 65 513 57 505 2.80e-62 213
Msa0832770 AT4G21910.4 29.111 450 317 2 65 513 57 505 2.80e-62 213
Msa0832770 AT3G26590.1 27.983 461 325 4 65 523 45 500 5.00e-62 212
Msa0832770 AT3G59030.1 29.630 459 315 2 61 515 47 501 6.76e-62 212
Msa0832770 AT1G61890.3 30.151 398 278 0 65 462 47 444 2.91e-61 209
Msa0832770 AT1G15170.2 34.384 349 219 4 161 504 11 354 1.10e-59 202
Msa0832770 AT1G23300.1 28.039 510 340 9 18 518 5 496 6.86e-59 204
Msa0832770 AT5G38030.1 27.765 443 311 5 65 503 45 482 1.40e-58 202
Msa0832770 AT4G00350.1 26.430 507 361 4 13 518 44 539 2.36e-57 200
Msa0832770 AT4G21903.1 28.205 429 306 2 88 515 76 503 1.38e-56 197
Msa0832770 AT3G23560.2 34.682 346 219 3 63 405 37 378 3.14e-56 194
Msa0832770 AT1G12950.1 27.354 446 311 5 65 504 67 505 8.08e-56 196
Msa0832770 AT5G10420.1 31.360 456 301 5 65 512 38 489 8.88e-56 195
Msa0832770 AT3G26590.2 27.595 395 281 3 126 518 4 395 1.69e-55 192
Msa0832770 AT1G47530.1 26.526 475 336 6 37 503 3 472 2.29e-55 194
Msa0832770 AT1G15150.2 31.111 360 244 2 161 518 11 368 3.19e-55 190
Msa0832770 AT4G21903.3 28.571 392 279 1 126 516 4 395 5.27e-54 187
Msa0832770 AT5G44050.1 30.512 449 292 8 65 503 40 478 6.92e-54 190
Msa0832770 AT4G21903.2 27.703 444 308 4 88 531 76 506 2.04e-53 189
Msa0832770 AT5G65380.1 30.258 466 306 9 47 502 18 474 2.09e-52 186
Msa0832770 AT1G66760.4 30.673 401 264 5 36 430 1 393 5.22e-52 182
Msa0832770 AT4G25640.1 29.231 455 316 2 64 514 33 485 2.20e-51 183
Msa0832770 AT1G33090.1 29.526 464 311 6 65 517 36 494 2.45e-51 183
Msa0832770 AT4G25640.2 29.231 455 316 2 64 514 33 485 2.87e-51 183
Msa0832770 AT1G33100.1 28.942 463 315 6 65 517 33 491 1.14e-50 181
Msa0832770 AT1G33110.1 28.419 468 321 8 39 498 7 468 3.55e-49 177
Msa0832770 AT1G33080.1 26.981 467 333 4 37 498 5 468 7.62e-48 174
Msa0832770 AT1G33080.2 26.981 467 333 4 37 498 5 468 1.69e-47 172
Msa0832770 AT3G59030.2 30.749 374 251 2 61 430 47 416 2.25e-46 168
Msa0832770 AT1G47530.2 26.702 382 271 5 127 504 5 381 3.13e-46 167
Msa0832770 AT5G17700.2 26.316 475 332 9 39 504 7 472 3.51e-45 166
Msa0832770 AT5G17700.1 26.316 475 332 9 39 504 7 472 3.51e-45 166
Msa0832770 AT3G03620.2 26.596 470 330 7 64 526 36 497 1.04e-43 162
Msa0832770 AT3G03620.1 26.596 470 330 7 64 526 36 497 1.04e-43 162
Msa0832770 AT4G25640.3 29.032 403 284 1 64 466 33 433 2.57e-43 160
Msa0832770 AT1G66760.3 31.268 339 217 6 168 498 3 333 2.74e-41 152
Msa0832770 AT1G33110.2 27.820 399 278 6 39 431 7 401 9.41e-35 135
Msa0832770 AT1G15180.2 32.692 260 169 2 161 417 11 267 1.81e-34 135
Msa0832770 AT5G17700.3 26.257 358 250 7 39 388 7 358 8.15e-30 121
Msa0832770 AT2G04066.1 32.258 124 82 1 381 504 40 161 2.83e-14 71.2

Find 95 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTTACATTTGGGTTTC+TGG 0.062617 5_4:+5902643 Msa0832770:CDS
AGGTTTGTGGTTTGGTCTTA+TGG 0.195458 5_4:+5903263 Msa0832770:CDS
TTTCCTTCTTCTTTGAGTTT+TGG 0.208924 5_4:+5902877 Msa0832770:CDS
TGTTTGAATGTTGGGGATTT+AGG 0.209468 5_4:-5901992 None:intergenic
TTAGGCGCAAATATAAATTT+AGG 0.214567 5_4:+5903174 Msa0832770:CDS
CCTATTTGTGGTCAAGCTTT+TGG 0.243633 5_4:+5902241 Msa0832770:CDS
TTGACAAGAAAATAGTTTAT+AGG 0.246031 5_4:-5902547 None:intergenic
GGTTACAATGTTGTTTGTTT+TGG 0.270469 5_4:+5903305 Msa0832770:CDS
ACATGCAACTGTTGCATCTA+TGG 0.280637 5_4:+5902819 Msa0832770:CDS
TTTCATTGATTATTTACATT+TGG 0.283279 5_4:+5902635 Msa0832770:CDS
TTCATTGATTATTTACATTT+GGG 0.294150 5_4:+5902636 Msa0832770:CDS
TTTATATTTGCGCCTAATTT+CGG 0.301593 5_4:-5903168 None:intergenic
GTTTGAATGTTGGGGATTTA+GGG 0.310488 5_4:-5901991 None:intergenic
TCTTGTCAATGTTCTTCAAT+TGG 0.313344 5_4:+5902561 Msa0832770:CDS
ATTGGATTTGCAAACATAAC+AGG 0.318839 5_4:+5902181 Msa0832770:CDS
GGAATAAGAGGAATCGCTTT+AGG 0.319468 5_4:+5902583 Msa0832770:CDS
AAAGTAGCACTATATGTTAA+AGG 0.335752 5_4:-5902505 None:intergenic
CTTGTCAATGTTCTTCAATT+GGG 0.346715 5_4:+5902562 Msa0832770:CDS
GTTTGGTTGAGTTTCTTTGC+TGG 0.349890 5_4:+5903228 Msa0832770:CDS
GATTTCAAAGGTTTGTGGTT+TGG 0.352902 5_4:+5903255 Msa0832770:CDS
TCAATGGTGTTACCGATTAT+AGG 0.360217 5_4:+5903081 Msa0832770:CDS
GTTCTTCAATTGGGAATAAG+AGG 0.367653 5_4:+5902571 Msa0832770:CDS
CCAAAAGCTTGACCACAAAT+AGG 0.369074 5_4:-5902241 None:intergenic
CGTTTGCTATATCTTCTTGT+TGG 0.374870 5_4:-5902384 None:intergenic
TGCTATGTTTGAAATATGTT+TGG 0.394695 5_4:-5902053 None:intergenic
ATCATTTCAATGTTGTTCCT+TGG 0.403969 5_4:+5902118 Msa0832770:CDS
CCTCTGCTTGTTTCAAAGGA+TGG 0.406928 5_4:+5902695 Msa0832770:CDS
AATGATTGTGCTACAAGATC+AGG 0.410062 5_4:-5902436 None:intergenic
TTGTGGATATATTATTTGTT+TGG 0.410448 5_4:-5901916 None:intergenic
AGCAATTCCAAGCTGCATTT+CGG 0.410449 5_4:+5902735 Msa0832770:CDS
CTCTGGTCTTGCCATGGGAA+TGG 0.412093 5_4:+5902216 Msa0832770:CDS
AATAATTGCTTTAACATCAA+TGG 0.417803 5_4:+5903065 Msa0832770:CDS
ATTTCCTCTGCTTGTTTCAA+AGG 0.418055 5_4:+5902691 Msa0832770:CDS
GAATCGCTTTAGGTTCCGTT+TGG 0.418185 5_4:+5902593 Msa0832770:CDS
GCTAAAAGATTCAAACTCCT+TGG 0.419870 5_4:+5902265 Msa0832770:CDS
GGAACGGCGAGGCCGAAATT+AGG 0.422373 5_4:+5903156 Msa0832770:CDS
ATATGTTAAAGGAAGTGTTA+TGG 0.428714 5_4:-5902494 None:intergenic
AAACTCAACCAAACTGCAAC+AGG 0.430260 5_4:-5903219 None:intergenic
AGCTCACATAGTCCTATAAT+CGG 0.440906 5_4:-5903093 None:intergenic
GCAGGTGGGTCACTTGCCAT+TGG 0.441197 5_4:+5902163 Msa0832770:CDS
CATTCTCTCTGGTCTTGCCA+TGG 0.443498 5_4:+5902210 Msa0832770:CDS
GCAAAACTTGCAGCAATAGT+TGG 0.450295 5_4:+5902949 Msa0832770:CDS
TTTGGTGTTTCAACAAGAGT+TGG 0.450548 5_4:+5902895 Msa0832770:CDS
GATGTTAAAGCAATTATTTG+TGG 0.453850 5_4:-5903060 None:intergenic
TGGGAATGCCTGTTGCAGTT+TGG 0.457141 5_4:+5903211 Msa0832770:CDS
GTTGGTGAGCTTGCTTTAGC+AGG 0.461842 5_4:+5902145 Msa0832770:CDS
ATCAACGGTGTTAACATGTC+AGG 0.462592 5_4:-5901968 None:intergenic
AAGGAACAACATTGAAATGA+TGG 0.463159 5_4:-5902116 None:intergenic
TTTGCTGGATTTGATTTCAA+AGG 0.464102 5_4:+5903243 Msa0832770:CDS
GATTTGATTTCAAAGGTTTG+TGG 0.465351 5_4:+5903250 Msa0832770:CDS
ATTCTCTCTGGTCTTGCCAT+GGG 0.465845 5_4:+5902211 Msa0832770:CDS
GGCTGCTCAAGGTTCATGTA+TGG 0.465974 5_4:+5903284 Msa0832770:CDS
TTAAAGGAAGTGTTATGGAT+TGG 0.467103 5_4:-5902489 None:intergenic
CGGTTTGTCTCGAATGGTGG+TGG 0.469476 5_4:+5902755 Msa0832770:CDS
CATGGCAAGACCAGAGAGAA+TGG 0.471209 5_4:-5902209 None:intergenic
GCATTTCGGTTTGTCTCGAA+TGG 0.473600 5_4:+5902749 Msa0832770:CDS
CCAAATGTTTCTAACAGAAA+AGG 0.479393 5_4:-5903016 None:intergenic
TTCAAACATAGCATTACCTA+TGG 0.482369 5_4:+5902063 Msa0832770:CDS
AATATGTTTGGCTTCATTGA+GGG 0.484804 5_4:-5902041 None:intergenic
TTCAAGTGAAGAAGAACAAG+AGG 0.495983 5_4:+5903383 Msa0832770:CDS
ACAGGTTACTCCATTCTCTC+TGG 0.501242 5_4:+5902199 Msa0832770:CDS
TTTGGTCTTATGGCTGCTCA+AGG 0.501334 5_4:+5903273 Msa0832770:CDS
ACACCACAAACAGTTGTTTG+TGG 0.502053 5_4:-5903126 None:intergenic
GTGAGCTTGCTTTAGCAGGT+GGG 0.506427 5_4:+5902149 Msa0832770:CDS
ACAAGAGTTGGAAATGAACT+TGG 0.513291 5_4:+5902907 Msa0832770:CDS
AAATATGTTTGGCTTCATTG+AGG 0.523182 5_4:-5902042 None:intergenic
ATTATAGGACTATGTGAGCT+TGG 0.533869 5_4:+5903096 Msa0832770:CDS
AATAAACCTGTTAAAACCAT+AGG 0.534613 5_4:-5902079 None:intergenic
CTTCGAAGATAGATTCTCAA+AGG 0.539810 5_4:-5902466 None:intergenic
CATGCAACTGTTGCATCTAT+GGG 0.545154 5_4:+5902820 Msa0832770:CDS
TGTCCACAAACAACTGTTTG+TGG 0.548402 5_4:+5903123 Msa0832770:CDS
ACACCAAAACTCAAAGAAGA+AGG 0.550180 5_4:-5902880 None:intergenic
AGACAAACCGAAATGCAGCT+TGG 0.556967 5_4:-5902742 None:intergenic
CTGGAACACACAAGAAAACA+TGG 0.559518 5_4:+5902662 Msa0832770:CDS
TTCTTTGCATTGTTAAACCA+AGG 0.562087 5_4:-5902282 None:intergenic
GGTGAGCTTGCTTTAGCAGG+TGG 0.562489 5_4:+5902148 Msa0832770:CDS
CCATCCTTTGAAACAAGCAG+AGG 0.563274 5_4:-5902695 None:intergenic
GTTATTGCTTTCATACAAAG+TGG 0.571039 5_4:-5901891 None:intergenic
ATGTTGTTCCTTGGTCGTGT+TGG 0.573577 5_4:+5902127 Msa0832770:CDS
TGTTATGTTTGCAAATCCAA+TGG 0.576457 5_4:-5902179 None:intergenic
TTCGAAGATAGATTCTCAAA+GGG 0.581553 5_4:-5902465 None:intergenic
GAGGTTGAAATTCGTCCAAA+CGG 0.591406 5_4:-5902608 None:intergenic
GTTGGGGATTTAGGGATCAA+CGG 0.596686 5_4:-5901983 None:intergenic
TTGTGGATCACTTGTGAACA+TGG 0.604296 5_4:-5903043 None:intergenic
TTTCGGTTTGTCTCGAATGG+TGG 0.608886 5_4:+5902752 Msa0832770:CDS
ATGGGAATGGAACCTATTTG+TGG 0.610601 5_4:+5902229 Msa0832770:CDS
AAATCAAGCAGAGAGAGCTA+AGG 0.614779 5_4:+5903344 Msa0832770:CDS
ACAAATAGGTTCCATTCCCA+TGG 0.616628 5_4:-5902227 None:intergenic
ACACACAAGAAAACATGGAG+TGG 0.626414 5_4:+5902667 Msa0832770:CDS
ATTATCAACTCAAGTTCATG+TGG 0.629140 5_4:+5903417 Msa0832770:CDS
CAAGCTCACCAACACGACCA+AGG 0.635498 5_4:-5902135 None:intergenic
TTCATACAAAGTGGAGACAG+AGG 0.650487 5_4:-5901882 None:intergenic
ATAGATGCAACAGTTGCATG+TGG 0.665559 5_4:-5902817 None:intergenic
AAATAATCAATGAAATAACG+AGG 0.708411 5_4:-5902627 None:intergenic
TGATGATGATAACTTGCACA+TGG 0.742527 5_4:-5901855 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTGATTATTTACATT+TGG + chr5_4:5902635-5902654 Msa0832770:CDS 15.0%
!!! TTCATTGATTATTTACATTT+GGG + chr5_4:5902636-5902655 Msa0832770:CDS 15.0%
!!! TTTCATTGATTATTTACATT+TGG + chr5_4:5902635-5902654 Msa0832770:CDS 15.0%
!!! TTCATTGATTATTTACATTT+GGG + chr5_4:5902636-5902655 Msa0832770:CDS 15.0%
!! TTGTGGATATATTATTTGTT+TGG - chr5_4:5901919-5901938 None:intergenic 20.0%
!!! CAATTTTCATTTCATTCTTA+TGG + chr5_4:5902329-5902348 Msa0832770:CDS 20.0%
!!! AAGAGATTATTACTTTTATG+TGG + chr5_4:5902361-5902380 Msa0832770:CDS 20.0%
!! TTGACAAGAAAATAGTTTAT+AGG - chr5_4:5902550-5902569 None:intergenic 20.0%
!! AAATAATCAATGAAATAACG+AGG - chr5_4:5902630-5902649 None:intergenic 20.0%
!! AATAATTGCTTTAACATCAA+TGG + chr5_4:5903065-5903084 Msa0832770:CDS 20.0%
!! TTGTGGATATATTATTTGTT+TGG - chr5_4:5901919-5901938 None:intergenic 20.0%
!!! CAATTTTCATTTCATTCTTA+TGG + chr5_4:5902329-5902348 Msa0832770:CDS 20.0%
!!! AAGAGATTATTACTTTTATG+TGG + chr5_4:5902361-5902380 Msa0832770:CDS 20.0%
!! TTGACAAGAAAATAGTTTAT+AGG - chr5_4:5902550-5902569 None:intergenic 20.0%
!! AAATAATCAATGAAATAACG+AGG - chr5_4:5902630-5902649 None:intergenic 20.0%
!! AATAATTGCTTTAACATCAA+TGG + chr5_4:5903065-5903084 Msa0832770:CDS 20.0%
!!! ATTATTCTGTGTTTTGATTG+TGG - chr5_4:5901942-5901961 None:intergenic 25.0%
!!! TCTTTTGTTGTTTGAATGTT+GGG - chr5_4:5902003-5902022 None:intergenic 25.0%
!!! TTCTTTTGTTGTTTGAATGT+TGG - chr5_4:5902004-5902023 None:intergenic 25.0%
!! TGCTATGTTTGAAATATGTT+TGG - chr5_4:5902056-5902075 None:intergenic 25.0%
! AATAAACCTGTTAAAACCAT+AGG - chr5_4:5902082-5902101 None:intergenic 25.0%
!!! ATATGTTAAAGGAAGTGTTA+TGG - chr5_4:5902497-5902516 None:intergenic 25.0%
! AAAGTAGCACTATATGTTAA+AGG - chr5_4:5902508-5902527 None:intergenic 25.0%
!! ATTATTTACATTTGGGTTTC+TGG + chr5_4:5902643-5902662 Msa0832770:CDS 25.0%
!! GAAATAATGATTTTGCTTTG+TGG + chr5_4:5902781-5902800 Msa0832770:CDS 25.0%
! AAAAATGTATATCAATGCAG+TGG - chr5_4:5902860-5902879 None:intergenic 25.0%
!! CTTTTCTGTTAGAAACATTT+GGG + chr5_4:5903017-5903036 Msa0832770:CDS 25.0%
! GATGTTAAAGCAATTATTTG+TGG - chr5_4:5903063-5903082 None:intergenic 25.0%
! TTTATATTTGCGCCTAATTT+CGG - chr5_4:5903171-5903190 None:intergenic 25.0%
! TTAGGCGCAAATATAAATTT+AGG + chr5_4:5903174-5903193 Msa0832770:CDS 25.0%
!!! TTTAGGTTGTTTTTATCTTG+TGG + chr5_4:5903191-5903210 Msa0832770:CDS 25.0%
!!! TTAGGTTGTTTTTATCTTGT+GGG + chr5_4:5903192-5903211 Msa0832770:CDS 25.0%
!!! ATTATTCTGTGTTTTGATTG+TGG - chr5_4:5901942-5901961 None:intergenic 25.0%
!!! TCTTTTGTTGTTTGAATGTT+GGG - chr5_4:5902003-5902022 None:intergenic 25.0%
!!! TTCTTTTGTTGTTTGAATGT+TGG - chr5_4:5902004-5902023 None:intergenic 25.0%
!! TGCTATGTTTGAAATATGTT+TGG - chr5_4:5902056-5902075 None:intergenic 25.0%
! AATAAACCTGTTAAAACCAT+AGG - chr5_4:5902082-5902101 None:intergenic 25.0%
!!! ATATGTTAAAGGAAGTGTTA+TGG - chr5_4:5902497-5902516 None:intergenic 25.0%
! AAAGTAGCACTATATGTTAA+AGG - chr5_4:5902508-5902527 None:intergenic 25.0%
!! ATTATTTACATTTGGGTTTC+TGG + chr5_4:5902643-5902662 Msa0832770:CDS 25.0%
!! GAAATAATGATTTTGCTTTG+TGG + chr5_4:5902781-5902800 Msa0832770:CDS 25.0%
! AAAAATGTATATCAATGCAG+TGG - chr5_4:5902860-5902879 None:intergenic 25.0%
!! CTTTTCTGTTAGAAACATTT+GGG + chr5_4:5903017-5903036 Msa0832770:CDS 25.0%
! GATGTTAAAGCAATTATTTG+TGG - chr5_4:5903063-5903082 None:intergenic 25.0%
! TTTATATTTGCGCCTAATTT+CGG - chr5_4:5903171-5903190 None:intergenic 25.0%
! TTAGGCGCAAATATAAATTT+AGG + chr5_4:5903174-5903193 Msa0832770:CDS 25.0%
!!! TTTAGGTTGTTTTTATCTTG+TGG + chr5_4:5903191-5903210 Msa0832770:CDS 25.0%
!!! TTAGGTTGTTTTTATCTTGT+GGG + chr5_4:5903192-5903211 Msa0832770:CDS 25.0%
GTTATTGCTTTCATACAAAG+TGG - chr5_4:5901894-5901913 None:intergenic 30.0%
!!! CTTTTGTTGTTTGAATGTTG+GGG - chr5_4:5902002-5902021 None:intergenic 30.0%
! AATATGTTTGGCTTCATTGA+GGG - chr5_4:5902044-5902063 None:intergenic 30.0%
! AAATATGTTTGGCTTCATTG+AGG - chr5_4:5902045-5902064 None:intergenic 30.0%
TTCAAACATAGCATTACCTA+TGG + chr5_4:5902063-5902082 Msa0832770:CDS 30.0%
AAGGAACAACATTGAAATGA+TGG - chr5_4:5902119-5902138 None:intergenic 30.0%
ATCATTTCAATGTTGTTCCT+TGG + chr5_4:5902118-5902137 Msa0832770:CDS 30.0%
TGTTATGTTTGCAAATCCAA+TGG - chr5_4:5902182-5902201 None:intergenic 30.0%
ATTGGATTTGCAAACATAAC+AGG + chr5_4:5902181-5902200 Msa0832770:CDS 30.0%
TTCTTTGCATTGTTAAACCA+AGG - chr5_4:5902285-5902304 None:intergenic 30.0%
TTCGAAGATAGATTCTCAAA+GGG - chr5_4:5902468-5902487 None:intergenic 30.0%
!! TTAAAGGAAGTGTTATGGAT+TGG - chr5_4:5902492-5902511 None:intergenic 30.0%
TCTTGTCAATGTTCTTCAAT+TGG + chr5_4:5902561-5902580 Msa0832770:CDS 30.0%
CTTGTCAATGTTCTTCAATT+GGG + chr5_4:5902562-5902581 Msa0832770:CDS 30.0%
!! TTTCCTTCTTCTTTGAGTTT+TGG + chr5_4:5902877-5902896 Msa0832770:CDS 30.0%
!!! ACTCTGTTTCAGTTTTGTTT+TGG + chr5_4:5902972-5902991 Msa0832770:CDS 30.0%
!!! CTCTGTTTCAGTTTTGTTTT+GGG + chr5_4:5902973-5902992 Msa0832770:CDS 30.0%
! CCAAATGTTTCTAACAGAAA+AGG - chr5_4:5903019-5903038 None:intergenic 30.0%
! CCTTTTCTGTTAGAAACATT+TGG + chr5_4:5903016-5903035 Msa0832770:CDS 30.0%
! TTTGCTGGATTTGATTTCAA+AGG + chr5_4:5903243-5903262 Msa0832770:CDS 30.0%
! GATTTGATTTCAAAGGTTTG+TGG + chr5_4:5903250-5903269 Msa0832770:CDS 30.0%
!! GGTTACAATGTTGTTTGTTT+TGG + chr5_4:5903305-5903324 Msa0832770:CDS 30.0%
!!! TTGTTTTGGTTCGTACAAAT+TGG + chr5_4:5903319-5903338 Msa0832770:CDS 30.0%
!!! TGTTTTGGTTCGTACAAATT+GGG + chr5_4:5903320-5903339 Msa0832770:CDS 30.0%
ATTATCAACTCAAGTTCATG+TGG + chr5_4:5903417-5903436 Msa0832770:CDS 30.0%
GTTATTGCTTTCATACAAAG+TGG - chr5_4:5901894-5901913 None:intergenic 30.0%
!!! CTTTTGTTGTTTGAATGTTG+GGG - chr5_4:5902002-5902021 None:intergenic 30.0%
! AATATGTTTGGCTTCATTGA+GGG - chr5_4:5902044-5902063 None:intergenic 30.0%
! AAATATGTTTGGCTTCATTG+AGG - chr5_4:5902045-5902064 None:intergenic 30.0%
TTCAAACATAGCATTACCTA+TGG + chr5_4:5902063-5902082 Msa0832770:CDS 30.0%
AAGGAACAACATTGAAATGA+TGG - chr5_4:5902119-5902138 None:intergenic 30.0%
ATCATTTCAATGTTGTTCCT+TGG + chr5_4:5902118-5902137 Msa0832770:CDS 30.0%
TGTTATGTTTGCAAATCCAA+TGG - chr5_4:5902182-5902201 None:intergenic 30.0%
ATTGGATTTGCAAACATAAC+AGG + chr5_4:5902181-5902200 Msa0832770:CDS 30.0%
TTCTTTGCATTGTTAAACCA+AGG - chr5_4:5902285-5902304 None:intergenic 30.0%
TTCGAAGATAGATTCTCAAA+GGG - chr5_4:5902468-5902487 None:intergenic 30.0%
!! TTAAAGGAAGTGTTATGGAT+TGG - chr5_4:5902492-5902511 None:intergenic 30.0%
TCTTGTCAATGTTCTTCAAT+TGG + chr5_4:5902561-5902580 Msa0832770:CDS 30.0%
CTTGTCAATGTTCTTCAATT+GGG + chr5_4:5902562-5902581 Msa0832770:CDS 30.0%
!! TTTCCTTCTTCTTTGAGTTT+TGG + chr5_4:5902877-5902896 Msa0832770:CDS 30.0%
!!! ACTCTGTTTCAGTTTTGTTT+TGG + chr5_4:5902972-5902991 Msa0832770:CDS 30.0%
!!! CTCTGTTTCAGTTTTGTTTT+GGG + chr5_4:5902973-5902992 Msa0832770:CDS 30.0%
! CCAAATGTTTCTAACAGAAA+AGG - chr5_4:5903019-5903038 None:intergenic 30.0%
! CCTTTTCTGTTAGAAACATT+TGG + chr5_4:5903016-5903035 Msa0832770:CDS 30.0%
! TTTGCTGGATTTGATTTCAA+AGG + chr5_4:5903243-5903262 Msa0832770:CDS 30.0%
! GATTTGATTTCAAAGGTTTG+TGG + chr5_4:5903250-5903269 Msa0832770:CDS 30.0%
!! GGTTACAATGTTGTTTGTTT+TGG + chr5_4:5903305-5903324 Msa0832770:CDS 30.0%
!!! TTGTTTTGGTTCGTACAAAT+TGG + chr5_4:5903319-5903338 Msa0832770:CDS 30.0%
!!! TGTTTTGGTTCGTACAAATT+GGG + chr5_4:5903320-5903339 Msa0832770:CDS 30.0%
ATTATCAACTCAAGTTCATG+TGG + chr5_4:5903417-5903436 Msa0832770:CDS 30.0%
GTTTGAATGTTGGGGATTTA+GGG - chr5_4:5901994-5902013 None:intergenic 35.0%
TGTTTGAATGTTGGGGATTT+AGG - chr5_4:5901995-5902014 None:intergenic 35.0%
!!! GCATTACCTATGGTTTTAAC+AGG + chr5_4:5902073-5902092 Msa0832770:CDS 35.0%
GCTAAAAGATTCAAACTCCT+TGG + chr5_4:5902265-5902284 Msa0832770:CDS 35.0%
!! CGTTTGCTATATCTTCTTGT+TGG - chr5_4:5902387-5902406 None:intergenic 35.0%
AATGATTGTGCTACAAGATC+AGG - chr5_4:5902439-5902458 None:intergenic 35.0%
CTTCGAAGATAGATTCTCAA+AGG - chr5_4:5902469-5902488 None:intergenic 35.0%
GTTCTTCAATTGGGAATAAG+AGG + chr5_4:5902571-5902590 Msa0832770:CDS 35.0%
ATTTCCTCTGCTTGTTTCAA+AGG + chr5_4:5902691-5902710 Msa0832770:CDS 35.0%
ACACCAAAACTCAAAGAAGA+AGG - chr5_4:5902883-5902902 None:intergenic 35.0%
!! TTTGGTGTTTCAACAAGAGT+TGG + chr5_4:5902895-5902914 Msa0832770:CDS 35.0%
ACAAGAGTTGGAAATGAACT+TGG + chr5_4:5902907-5902926 Msa0832770:CDS 35.0%
AGAAAAGGCGAAAAACAAAG+CGG - chr5_4:5903004-5903023 None:intergenic 35.0%
!! TCAATGGTGTTACCGATTAT+AGG + chr5_4:5903081-5903100 Msa0832770:CDS 35.0%
AGCTCACATAGTCCTATAAT+CGG - chr5_4:5903096-5903115 None:intergenic 35.0%
ATTATAGGACTATGTGAGCT+TGG + chr5_4:5903096-5903115 Msa0832770:CDS 35.0%
!! AACTGTTTGTGGTGTTTTGA+GGG + chr5_4:5903134-5903153 Msa0832770:CDS 35.0%
GATTTCAAAGGTTTGTGGTT+TGG + chr5_4:5903255-5903274 Msa0832770:CDS 35.0%
TTCAAGTGAAGAAGAACAAG+AGG + chr5_4:5903383-5903402 Msa0832770:CDS 35.0%
GTTTGAATGTTGGGGATTTA+GGG - chr5_4:5901994-5902013 None:intergenic 35.0%
TGTTTGAATGTTGGGGATTT+AGG - chr5_4:5901995-5902014 None:intergenic 35.0%
!!! GCATTACCTATGGTTTTAAC+AGG + chr5_4:5902073-5902092 Msa0832770:CDS 35.0%
GCTAAAAGATTCAAACTCCT+TGG + chr5_4:5902265-5902284 Msa0832770:CDS 35.0%
!! CGTTTGCTATATCTTCTTGT+TGG - chr5_4:5902387-5902406 None:intergenic 35.0%
AATGATTGTGCTACAAGATC+AGG - chr5_4:5902439-5902458 None:intergenic 35.0%
CTTCGAAGATAGATTCTCAA+AGG - chr5_4:5902469-5902488 None:intergenic 35.0%
GTTCTTCAATTGGGAATAAG+AGG + chr5_4:5902571-5902590 Msa0832770:CDS 35.0%
ATTTCCTCTGCTTGTTTCAA+AGG + chr5_4:5902691-5902710 Msa0832770:CDS 35.0%
ACACCAAAACTCAAAGAAGA+AGG - chr5_4:5902883-5902902 None:intergenic 35.0%
!! TTTGGTGTTTCAACAAGAGT+TGG + chr5_4:5902895-5902914 Msa0832770:CDS 35.0%
ACAAGAGTTGGAAATGAACT+TGG + chr5_4:5902907-5902926 Msa0832770:CDS 35.0%
AGAAAAGGCGAAAAACAAAG+CGG - chr5_4:5903004-5903023 None:intergenic 35.0%
!! TCAATGGTGTTACCGATTAT+AGG + chr5_4:5903081-5903100 Msa0832770:CDS 35.0%
AGCTCACATAGTCCTATAAT+CGG - chr5_4:5903096-5903115 None:intergenic 35.0%
ATTATAGGACTATGTGAGCT+TGG + chr5_4:5903096-5903115 Msa0832770:CDS 35.0%
!! AACTGTTTGTGGTGTTTTGA+GGG + chr5_4:5903134-5903153 Msa0832770:CDS 35.0%
GATTTCAAAGGTTTGTGGTT+TGG + chr5_4:5903255-5903274 Msa0832770:CDS 35.0%
TTCAAGTGAAGAAGAACAAG+AGG + chr5_4:5903383-5903402 Msa0832770:CDS 35.0%
TTCATACAAAGTGGAGACAG+AGG - chr5_4:5901885-5901904 None:intergenic 40.0%
!!! CTGTGTTTTGATTGTGGTTG+TGG - chr5_4:5901936-5901955 None:intergenic 40.0%
! ATCAACGGTGTTAACATGTC+AGG - chr5_4:5901971-5901990 None:intergenic 40.0%
ATTGAGGGCGAGAGAAAAAT+GGG - chr5_4:5902029-5902048 None:intergenic 40.0%
ACAAATAGGTTCCATTCCCA+TGG - chr5_4:5902230-5902249 None:intergenic 40.0%
ATGGGAATGGAACCTATTTG+TGG + chr5_4:5902229-5902248 Msa0832770:CDS 40.0%
CCAAAAGCTTGACCACAAAT+AGG - chr5_4:5902244-5902263 None:intergenic 40.0%
! CCTATTTGTGGTCAAGCTTT+TGG + chr5_4:5902241-5902260 Msa0832770:CDS 40.0%
GGAATAAGAGGAATCGCTTT+AGG + chr5_4:5902583-5902602 Msa0832770:CDS 40.0%
GAGGTTGAAATTCGTCCAAA+CGG - chr5_4:5902611-5902630 None:intergenic 40.0%
CTGGAACACACAAGAAAACA+TGG + chr5_4:5902662-5902681 Msa0832770:CDS 40.0%
ACACACAAGAAAACATGGAG+TGG + chr5_4:5902667-5902686 Msa0832770:CDS 40.0%
AGCAATTCCAAGCTGCATTT+CGG + chr5_4:5902735-5902754 Msa0832770:CDS 40.0%
ATAGATGCAACAGTTGCATG+TGG - chr5_4:5902820-5902839 None:intergenic 40.0%
ACATGCAACTGTTGCATCTA+TGG + chr5_4:5902819-5902838 Msa0832770:CDS 40.0%
CATGCAACTGTTGCATCTAT+GGG + chr5_4:5902820-5902839 Msa0832770:CDS 40.0%
!!! GCTGCAAGTTTTGCTTTTTG+TGG - chr5_4:5902943-5902962 None:intergenic 40.0%
GCAAAACTTGCAGCAATAGT+TGG + chr5_4:5902949-5902968 Msa0832770:CDS 40.0%
TTGTGGATCACTTGTGAACA+TGG - chr5_4:5903046-5903065 None:intergenic 40.0%
TGTCCACAAACAACTGTTTG+TGG + chr5_4:5903123-5903142 Msa0832770:CDS 40.0%
ACACCACAAACAGTTGTTTG+TGG - chr5_4:5903129-5903148 None:intergenic 40.0%
! CAACTGTTTGTGGTGTTTTG+AGG + chr5_4:5903133-5903152 Msa0832770:CDS 40.0%
!! ACTGTTTGTGGTGTTTTGAG+GGG + chr5_4:5903135-5903154 Msa0832770:CDS 40.0%
AAACTCAACCAAACTGCAAC+AGG - chr5_4:5903222-5903241 None:intergenic 40.0%
GTTTGGTTGAGTTTCTTTGC+TGG + chr5_4:5903228-5903247 Msa0832770:CDS 40.0%
!! AGGTTTGTGGTTTGGTCTTA+TGG + chr5_4:5903263-5903282 Msa0832770:CDS 40.0%
AAATCAAGCAGAGAGAGCTA+AGG + chr5_4:5903344-5903363 Msa0832770:CDS 40.0%
TTCATACAAAGTGGAGACAG+AGG - chr5_4:5901885-5901904 None:intergenic 40.0%
!!! CTGTGTTTTGATTGTGGTTG+TGG - chr5_4:5901936-5901955 None:intergenic 40.0%
! ATCAACGGTGTTAACATGTC+AGG - chr5_4:5901971-5901990 None:intergenic 40.0%
ATTGAGGGCGAGAGAAAAAT+GGG - chr5_4:5902029-5902048 None:intergenic 40.0%
ACAAATAGGTTCCATTCCCA+TGG - chr5_4:5902230-5902249 None:intergenic 40.0%
ATGGGAATGGAACCTATTTG+TGG + chr5_4:5902229-5902248 Msa0832770:CDS 40.0%
CCAAAAGCTTGACCACAAAT+AGG - chr5_4:5902244-5902263 None:intergenic 40.0%
! CCTATTTGTGGTCAAGCTTT+TGG + chr5_4:5902241-5902260 Msa0832770:CDS 40.0%
GGAATAAGAGGAATCGCTTT+AGG + chr5_4:5902583-5902602 Msa0832770:CDS 40.0%
GAGGTTGAAATTCGTCCAAA+CGG - chr5_4:5902611-5902630 None:intergenic 40.0%
CTGGAACACACAAGAAAACA+TGG + chr5_4:5902662-5902681 Msa0832770:CDS 40.0%
ACACACAAGAAAACATGGAG+TGG + chr5_4:5902667-5902686 Msa0832770:CDS 40.0%
AGCAATTCCAAGCTGCATTT+CGG + chr5_4:5902735-5902754 Msa0832770:CDS 40.0%
ATAGATGCAACAGTTGCATG+TGG - chr5_4:5902820-5902839 None:intergenic 40.0%
ACATGCAACTGTTGCATCTA+TGG + chr5_4:5902819-5902838 Msa0832770:CDS 40.0%
CATGCAACTGTTGCATCTAT+GGG + chr5_4:5902820-5902839 Msa0832770:CDS 40.0%
!!! GCTGCAAGTTTTGCTTTTTG+TGG - chr5_4:5902943-5902962 None:intergenic 40.0%
GCAAAACTTGCAGCAATAGT+TGG + chr5_4:5902949-5902968 Msa0832770:CDS 40.0%
TTGTGGATCACTTGTGAACA+TGG - chr5_4:5903046-5903065 None:intergenic 40.0%
TGTCCACAAACAACTGTTTG+TGG + chr5_4:5903123-5903142 Msa0832770:CDS 40.0%
ACACCACAAACAGTTGTTTG+TGG - chr5_4:5903129-5903148 None:intergenic 40.0%
! CAACTGTTTGTGGTGTTTTG+AGG + chr5_4:5903133-5903152 Msa0832770:CDS 40.0%
!! ACTGTTTGTGGTGTTTTGAG+GGG + chr5_4:5903135-5903154 Msa0832770:CDS 40.0%
AAACTCAACCAAACTGCAAC+AGG - chr5_4:5903222-5903241 None:intergenic 40.0%
GTTTGGTTGAGTTTCTTTGC+TGG + chr5_4:5903228-5903247 Msa0832770:CDS 40.0%
!! AGGTTTGTGGTTTGGTCTTA+TGG + chr5_4:5903263-5903282 Msa0832770:CDS 40.0%
AAATCAAGCAGAGAGAGCTA+AGG + chr5_4:5903344-5903363 Msa0832770:CDS 40.0%
GTTGGGGATTTAGGGATCAA+CGG - chr5_4:5901986-5902005 None:intergenic 45.0%
CATTGAGGGCGAGAGAAAAA+TGG - chr5_4:5902030-5902049 None:intergenic 45.0%
ATGTTGTTCCTTGGTCGTGT+TGG + chr5_4:5902127-5902146 Msa0832770:CDS 45.0%
ACAGGTTACTCCATTCTCTC+TGG + chr5_4:5902199-5902218 Msa0832770:CDS 45.0%
!! ATTCTCTCTGGTCTTGCCAT+GGG + chr5_4:5902211-5902230 Msa0832770:CDS 45.0%
! GAATCGCTTTAGGTTCCGTT+TGG + chr5_4:5902593-5902612 Msa0832770:CDS 45.0%
!! CCATCCTTTGAAACAAGCAG+AGG - chr5_4:5902698-5902717 None:intergenic 45.0%
CCTCTGCTTGTTTCAAAGGA+TGG + chr5_4:5902695-5902714 Msa0832770:CDS 45.0%
AGACAAACCGAAATGCAGCT+TGG - chr5_4:5902745-5902764 None:intergenic 45.0%
! GCATTTCGGTTTGTCTCGAA+TGG + chr5_4:5902749-5902768 Msa0832770:CDS 45.0%
! TTTCGGTTTGTCTCGAATGG+TGG + chr5_4:5902752-5902771 Msa0832770:CDS 45.0%
!!! TTGTGGTGTTTTGAGGGGAA+CGG + chr5_4:5903140-5903159 Msa0832770:CDS 45.0%
!! TTTGGTCTTATGGCTGCTCA+AGG + chr5_4:5903273-5903292 Msa0832770:CDS 45.0%
GTTGGGGATTTAGGGATCAA+CGG - chr5_4:5901986-5902005 None:intergenic 45.0%
CATTGAGGGCGAGAGAAAAA+TGG - chr5_4:5902030-5902049 None:intergenic 45.0%
ATGTTGTTCCTTGGTCGTGT+TGG + chr5_4:5902127-5902146 Msa0832770:CDS 45.0%
ACAGGTTACTCCATTCTCTC+TGG + chr5_4:5902199-5902218 Msa0832770:CDS 45.0%
!! ATTCTCTCTGGTCTTGCCAT+GGG + chr5_4:5902211-5902230 Msa0832770:CDS 45.0%
! GAATCGCTTTAGGTTCCGTT+TGG + chr5_4:5902593-5902612 Msa0832770:CDS 45.0%
!! CCATCCTTTGAAACAAGCAG+AGG - chr5_4:5902698-5902717 None:intergenic 45.0%
CCTCTGCTTGTTTCAAAGGA+TGG + chr5_4:5902695-5902714 Msa0832770:CDS 45.0%
AGACAAACCGAAATGCAGCT+TGG - chr5_4:5902745-5902764 None:intergenic 45.0%
! GCATTTCGGTTTGTCTCGAA+TGG + chr5_4:5902749-5902768 Msa0832770:CDS 45.0%
! TTTCGGTTTGTCTCGAATGG+TGG + chr5_4:5902752-5902771 Msa0832770:CDS 45.0%
!!! TTGTGGTGTTTTGAGGGGAA+CGG + chr5_4:5903140-5903159 Msa0832770:CDS 45.0%
!! TTTGGTCTTATGGCTGCTCA+AGG + chr5_4:5903273-5903292 Msa0832770:CDS 45.0%
! GTTGGTGAGCTTGCTTTAGC+AGG + chr5_4:5902145-5902164 Msa0832770:CDS 50.0%
! GTGAGCTTGCTTTAGCAGGT+GGG + chr5_4:5902149-5902168 Msa0832770:CDS 50.0%
CATGGCAAGACCAGAGAGAA+TGG - chr5_4:5902212-5902231 None:intergenic 50.0%
!! CATTCTCTCTGGTCTTGCCA+TGG + chr5_4:5902210-5902229 Msa0832770:CDS 50.0%
TGGGAATGCCTGTTGCAGTT+TGG + chr5_4:5903211-5903230 Msa0832770:CDS 50.0%
GGCTGCTCAAGGTTCATGTA+TGG + chr5_4:5903284-5903303 Msa0832770:CDS 50.0%
! GTTGGTGAGCTTGCTTTAGC+AGG + chr5_4:5902145-5902164 Msa0832770:CDS 50.0%
! GTGAGCTTGCTTTAGCAGGT+GGG + chr5_4:5902149-5902168 Msa0832770:CDS 50.0%
CATGGCAAGACCAGAGAGAA+TGG - chr5_4:5902212-5902231 None:intergenic 50.0%
!! CATTCTCTCTGGTCTTGCCA+TGG + chr5_4:5902210-5902229 Msa0832770:CDS 50.0%
TGGGAATGCCTGTTGCAGTT+TGG + chr5_4:5903211-5903230 Msa0832770:CDS 50.0%
GGCTGCTCAAGGTTCATGTA+TGG + chr5_4:5903284-5903303 Msa0832770:CDS 50.0%
CAAGCTCACCAACACGACCA+AGG - chr5_4:5902138-5902157 None:intergenic 55.0%
! GGTGAGCTTGCTTTAGCAGG+TGG + chr5_4:5902148-5902167 Msa0832770:CDS 55.0%
!! CTCTGGTCTTGCCATGGGAA+TGG + chr5_4:5902216-5902235 Msa0832770:CDS 55.0%
CGGTTTGTCTCGAATGGTGG+TGG + chr5_4:5902755-5902774 Msa0832770:CDS 55.0%
CAAGCTCACCAACACGACCA+AGG - chr5_4:5902138-5902157 None:intergenic 55.0%
! GGTGAGCTTGCTTTAGCAGG+TGG + chr5_4:5902148-5902167 Msa0832770:CDS 55.0%
!! CTCTGGTCTTGCCATGGGAA+TGG + chr5_4:5902216-5902235 Msa0832770:CDS 55.0%
CGGTTTGTCTCGAATGGTGG+TGG + chr5_4:5902755-5902774 Msa0832770:CDS 55.0%
GCAGGTGGGTCACTTGCCAT+TGG + chr5_4:5902163-5902182 Msa0832770:CDS 60.0%
!!! GTGTTTTGAGGGGAACGGCG+AGG + chr5_4:5903145-5903164 Msa0832770:CDS 60.0%
GGAACGGCGAGGCCGAAATT+AGG + chr5_4:5903156-5903175 Msa0832770:CDS 60.0%
GCAGGTGGGTCACTTGCCAT+TGG + chr5_4:5902163-5902182 Msa0832770:CDS 60.0%
!!! GTGTTTTGAGGGGAACGGCG+AGG + chr5_4:5903145-5903164 Msa0832770:CDS 60.0%
GGAACGGCGAGGCCGAAATT+AGG + chr5_4:5903156-5903175 Msa0832770:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 5901857 5903470 5901857 ID=Msa0832770;Name=Msa0832770
chr5_4 mRNA 5901857 5903470 5901857 ID=Msa0832770-mRNA-1;Parent=Msa0832770;Name=Msa0832770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|537
chr5_4 exon 5901857 5903470 5901857 ID=Msa0832770-mRNA-1:exon:2541;Parent=Msa0832770-mRNA-1
chr5_4 CDS 5901857 5903470 5901857 ID=Msa0832770-mRNA-1:cds;Parent=Msa0832770-mRNA-1
Gene Sequence

>Msa0832770

ATGTGCAAGTTATCATCATCATCAACCTCTGTCTCCACTTTGTATGAAAGCAATAACAACCAAACAAATAATATATCCACAACCACAATCAAAACACAGAATAATATCAAACCTGACATGTTAACACCGTTGATCCCTAAATCCCCAACATTCAAACAACAAAAGAAAACCCATTTTTCTCTCGCCCTCAATGAAGCCAAACATATTTCAAACATAGCATTACCTATGGTTTTAACAGGTTTATTACTTTATTCTCGTTCCATCATTTCAATGTTGTTCCTTGGTCGTGTTGGTGAGCTTGCTTTAGCAGGTGGGTCACTTGCCATTGGATTTGCAAACATAACAGGTTACTCCATTCTCTCTGGTCTTGCCATGGGAATGGAACCTATTTGTGGTCAAGCTTTTGGTGCTAAAAGATTCAAACTCCTTGGTTTAACAATGCAAAGAACAGTGATTCTTCTTCTTGTAACTTCAATTTTCATTTCATTCTTATGGCTTAACATGAAGAGATTATTACTTTTATGTGGCCAACAAGAAGATATAGCAAACGTTGCACAATCTTACATTCTTTATTCTCTTCCTGATCTTGTAGCACAATCATTGCTACACCCTTTGAGAATCTATCTTCGAAGCCAATCCATAACACTTCCTTTAACATATAGTGCTACTTTGTCTATTCTTCTTCACATTCCTATAAACTATTTTCTTGTCAATGTTCTTCAATTGGGAATAAGAGGAATCGCTTTAGGTTCCGTTTGGACGAATTTCAACCTCGTTATTTCATTGATTATTTACATTTGGGTTTCTGGAACACACAAGAAAACATGGAGTGGAATTTCCTCTGCTTGTTTCAAAGGATGGAAATCGCTTTTGAATTTAGCAATTCCAAGCTGCATTTCGGTTTGTCTCGAATGGTGGTGGTACGAAATAATGATTTTGCTTTGTGGATTATTGCTTAATCCACATGCAACTGTTGCATCTATGGGTGTGTTGATTCAAACCACTGCATTGATATACATTTTTCCTTCTTCTTTGAGTTTTGGTGTTTCAACAAGAGTTGGAAATGAACTTGGTGCTGAAAATCCACAAAAAGCAAAACTTGCAGCAATAGTTGGACTCTGTTTCAGTTTTGTTTTGGGTTTCTCCGCTTTGTTTTTCGCCTTTTCTGTTAGAAACATTTGGGCTACCATGTTCACAAGTGATCCACAAATAATTGCTTTAACATCAATGGTGTTACCGATTATAGGACTATGTGAGCTTGGAAATTGTCCACAAACAACTGTTTGTGGTGTTTTGAGGGGAACGGCGAGGCCGAAATTAGGCGCAAATATAAATTTAGGTTGTTTTTATCTTGTGGGAATGCCTGTTGCAGTTTGGTTGAGTTTCTTTGCTGGATTTGATTTCAAAGGTTTGTGGTTTGGTCTTATGGCTGCTCAAGGTTCATGTATGGTTACAATGTTGTTTGTTTTGGTTCGTACAAATTGGGAAAATCAAGCAGAGAGAGCTAAGGAGTTAACATCATCAGATTCAAGTGAAGAAGAACAAGAGGAAGAGAAAGTGATTATCAACTCAAGTTCATGTGGCACAAAAGAGTGCTCTGATTCATTAGTTTGA
Protein sequence

>Msa0832770

MCKLSSSSTSVSTLYESNNNQTNNISTTTIKTQNNIKPDMLTPLIPKSPTFKQQKKTHFSLALNEAKHISNIALPMVLTGLLLYSRSIISMLFLGRVGELALAGGSLAIGFANITGYSILSGLAMGMEPICGQAFGAKRFKLLGLTMQRTVILLLVTSIFISFLWLNMKRLLLLCGQQEDIANVAQSYILYSLPDLVAQSLLHPLRIYLRSQSITLPLTYSATLSILLHIPINYFLVNVLQLGIRGIALGSVWTNFNLVISLIIYIWVSGTHKKTWSGISSACFKGWKSLLNLAIPSCISVCLEWWWYEIMILLCGLLLNPHATVASMGVLIQTTALIYIFPSSLSFGVSTRVGNELGAENPQKAKLAAIVGLCFSFVLGFSALFFAFSVRNIWATMFTSDPQIIALTSMVLPIIGLCELGNCPQTTVCGVLRGTARPKLGANINLGCFYLVGMPVAVWLSFFAGFDFKGLWFGLMAAQGSCMVTMLFVLVRTNWENQAERAKELTSSDSSEEEQEEEKVIINSSSCGTKECSDSLV*