AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0838770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0838770 MtrunA17_Chr5g0406301 98.522 203 3 0 1 203 1 203 4.48e-149 412
Msa0838770 MtrunA17_Chr7g0256341 45.930 172 93 0 14 185 51 222 7.23e-57 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0838770 AT4G35750.1 75.369 203 49 1 1 203 1 202 1.96e-117 332
Msa0838770 AT3G10210.1 47.590 166 87 0 14 179 49 214 8.59e-54 172

Find 66 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATCATGCTAGGGTTTA+TGG 0.201786 5_4:+13292213 Msa0838770:CDS
ATCTCATAGGGTTTAGGATT+TGG 0.237359 5_4:+13292285 Msa0838770:exon
AGAAGAACTGCTTGAGAAAT+TGG 0.241731 5_4:+13291358 Msa0838770:CDS
TCTCATAGGGTTTAGGATTT+GGG 0.254159 5_4:+13292286 Msa0838770:exon
TCAGCAGAGTTGGTTATCTA+TGG 0.276388 5_4:+13292094 Msa0838770:CDS
GTTATCTATGGGAGAATGTT+AGG 0.282831 5_4:+13292106 Msa0838770:CDS
CATCTTGATATTAATAGTTA+TGG 0.321706 5_4:+13292580 Msa0838770:three_prime_UTR
TATCGTCCAATGATGGATTA+TGG 0.323869 5_4:+13292183 Msa0838770:CDS
GGCCAAACGTAGCGAGAAAA+AGG 0.325265 5_4:-13291765 None:intergenic
AGGTGTATCTCATAGGGTTT+AGG 0.338603 5_4:+13292279 Msa0838770:exon
CTCCATGAGGTGTATCTCAT+AGG 0.352468 5_4:+13292272 Msa0838770:CDS
AATTTCTTCTTCCCTAGCTT+CGG 0.354119 5_4:-13291595 None:intergenic
CGCAAGATCCTCCGCATTAT+CGG 0.357451 5_4:+13291416 Msa0838770:CDS
ATCGGTTTACGATGCGATTC+CGG 0.372506 5_4:+13291684 Msa0838770:CDS
ACAGAGCTTTCAAATAGTTA+TGG 0.379055 5_4:+13292435 Msa0838770:three_prime_UTR
ATACGATCATGATGAAGATT+TGG 0.383633 5_4:+13292158 Msa0838770:CDS
TTCTTGTTCAAGCTCAGTTA+TGG 0.385949 5_4:-13291339 None:intergenic
ACATTATATCTCAATAACTA+TGG 0.395608 5_4:-13292468 None:intergenic
TGCGTAGTGATAGAAGAAAA+AGG 0.406840 5_4:-13291290 None:intergenic
GAGGAACGAAGTGGAGATTC+CGG 0.411726 5_4:+13292128 Msa0838770:CDS
ATTTCTTCTTCCCTAGCTTC+GGG 0.419960 5_4:-13291594 None:intergenic
CGGAATTTCTTCTCTCCGAT+CGG 0.427792 5_4:+13291666 Msa0838770:CDS
CGATCGGAGAGAAGAAATTC+CGG 0.432814 5_4:-13291665 None:intergenic
AGATTCTCCTTCACGTTCGC+CGG 0.437993 5_4:-13291703 None:intergenic
TCTAATCCATAATCCATCAT+TGG 0.455280 5_4:-13292189 None:intergenic
TTTGGAGTATCGTCCAATGA+TGG 0.485084 5_4:+13292176 Msa0838770:CDS
GTTCACTGTAGTCGTTGGAT+TGG 0.491143 5_4:+13292308 Msa0838770:three_prime_UTR
TTTGGGTTCACTGTAGTCGT+TGG 0.495682 5_4:+13292303 Msa0838770:three_prime_UTR
TTGGGTGTGATAGGTTGTAC+AGG 0.501143 5_4:+13292058 Msa0838770:intron
GATTAGAGAGTGATCATGCT+AGG 0.515483 5_4:+13292205 Msa0838770:CDS
TCCATGAGGTGTATCTCATA+GGG 0.515766 5_4:+13292273 Msa0838770:CDS
CTGAGGTACATCAGCAGAGT+TGG 0.517318 5_4:+13292084 Msa0838770:CDS
ACCCAGCGTTGAAGAGAAAA+CGG 0.517837 5_4:-13291786 None:intergenic
CAAGACCTAAAGAAAACAAA+AGG 0.523861 5_4:-13292521 None:intergenic
TTGGAAGTCTTCAAAATCAA+AGG 0.533957 5_4:+13291377 Msa0838770:CDS
AGCGCGATTGGTTAGCGTTG+AGG 0.536404 5_4:+13291540 Msa0838770:intron
CTTTCAAATAGTTATGGAGA+TGG 0.537831 5_4:+13292441 Msa0838770:three_prime_UTR
TTGTTCAAGCTCAGTTATGG+TGG 0.540444 5_4:-13291336 None:intergenic
CAGCAGAGTTGGTTATCTAT+GGG 0.551346 5_4:+13292095 Msa0838770:CDS
GATGGTGAATGTGGATGGAT+AGG 0.553627 5_4:-13291231 None:intergenic
ATGGTGAATGTGGATGGATA+GGG 0.558835 5_4:-13291230 None:intergenic
GTCACAGGCGAATCTAGTGC+AGG 0.559387 5_4:-13292243 None:intergenic
AAAAGGCGAGCTTGTAAACC+AGG 0.560268 5_4:-13291748 None:intergenic
TATGATTGTGATGGTGAATG+TGG 0.563886 5_4:-13291240 None:intergenic
TCAAATAGTTATGGAGATGG+AGG 0.568318 5_4:+13292444 Msa0838770:three_prime_UTR
ATTATCGGAAAATTCTTCCC+TGG 0.573008 5_4:+13291431 Msa0838770:CDS
CGATAATGCGGAGGATCTTG+CGG 0.574332 5_4:-13291415 None:intergenic
CAAATAGTTATGGAGATGGA+GGG 0.578325 5_4:+13292445 Msa0838770:three_prime_UTR
TTGATATTAATAGTTATGGA+AGG 0.590651 5_4:+13292584 Msa0838770:three_prime_UTR
TGCGATTCCGGCGAACGTGA+AGG 0.593355 5_4:+13291696 Msa0838770:CDS
ATTAGAGAGTGATCATGCTA+GGG 0.594329 5_4:+13292206 Msa0838770:CDS
CTCATGGAGTAAGTAGTCAC+AGG 0.597762 5_4:-13292258 None:intergenic
ACCCTATGAGATACACCTCA+TGG 0.597933 5_4:-13292274 None:intergenic
ATTGTGATGGTGAATGTGGA+TGG 0.600099 5_4:-13291236 None:intergenic
TCATGATCGTATACGAACTC+CGG 0.600493 5_4:-13292147 None:intergenic
GAATGTTAGGAGGAACGAAG+TGG 0.610161 5_4:+13292119 Msa0838770:CDS
CGTGATGATTATGATTGTGA+TGG 0.623135 5_4:-13291249 None:intergenic
ATATTCACACCGGCGTTCAG+AGG 0.623653 5_4:+13291629 Msa0838770:CDS
ATCAAAGGCAAAGATAAGCA+TGG 0.637350 5_4:+13291392 Msa0838770:CDS
GAATCGCATCGTAAACCGAT+CGG 0.641309 5_4:-13291681 None:intergenic
ATCTATGGGAGAATGTTAGG+AGG 0.655849 5_4:+13292109 Msa0838770:CDS
ATAGGTTGTACAGGAAGCTG+AGG 0.667654 5_4:+13292067 Msa0838770:intron
ATTCTCGCTCCTCTGAACGC+CGG 0.670180 5_4:-13291638 None:intergenic
GCTAGGGAAGAAGAAATTCG+CGG 0.672425 5_4:+13291600 Msa0838770:CDS
CTGTGACTACTTACTCCATG+AGG 0.695115 5_4:+13292259 Msa0838770:CDS
GCGGTGTTGTATATTCACAC+CGG 0.721011 5_4:+13291619 Msa0838770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAATCACATATATAA+CGG - chr5_4:13291482-13291501 None:intergenic 15.0%
!!! GATATTTTCATTTTTTTTGT+TGG + chr5_4:13292606-13292625 Msa0838770:three_prime_UTR 15.0%
!! AACAAAAATCACATATATAA+CGG - chr5_4:13291482-13291501 None:intergenic 15.0%
!!! GATATTTTCATTTTTTTTGT+TGG + chr5_4:13292606-13292625 Msa0838770:three_prime_UTR 15.0%
!!! AACTGAAATAATTTTTACCA+GGG - chr5_4:13291451-13291470 None:intergenic 20.0%
!!! AAACTGAAATAATTTTTACC+AGG - chr5_4:13291452-13291471 None:intergenic 20.0%
!!! GATTAATTTAAAGCTGATTT+AGG - chr5_4:13291869-13291888 None:intergenic 20.0%
!! AAATTAATGAAGTTGTTTGT+AGG + chr5_4:13291989-13292008 Msa0838770:intron 20.0%
!!! TATTAATCGGAAGTTTTAAT+TGG + chr5_4:13292018-13292037 Msa0838770:intron 20.0%
!! ACATTATATCTCAATAACTA+TGG - chr5_4:13292471-13292490 None:intergenic 20.0%
!! CATCTTGATATTAATAGTTA+TGG + chr5_4:13292580-13292599 Msa0838770:three_prime_UTR 20.0%
!! TTGATATTAATAGTTATGGA+AGG + chr5_4:13292584-13292603 Msa0838770:three_prime_UTR 20.0%
!!! TTTTTTGTTGGATAATTCAA+TGG + chr5_4:13292618-13292637 Msa0838770:three_prime_UTR 20.0%
!!! AACTGAAATAATTTTTACCA+GGG - chr5_4:13291451-13291470 None:intergenic 20.0%
!!! AAACTGAAATAATTTTTACC+AGG - chr5_4:13291452-13291471 None:intergenic 20.0%
!!! GATTAATTTAAAGCTGATTT+AGG - chr5_4:13291869-13291888 None:intergenic 20.0%
!! AAATTAATGAAGTTGTTTGT+AGG + chr5_4:13291989-13292008 Msa0838770:intron 20.0%
!!! TATTAATCGGAAGTTTTAAT+TGG + chr5_4:13292018-13292037 Msa0838770:intron 20.0%
!! ACATTATATCTCAATAACTA+TGG - chr5_4:13292471-13292490 None:intergenic 20.0%
!! CATCTTGATATTAATAGTTA+TGG + chr5_4:13292580-13292599 Msa0838770:three_prime_UTR 20.0%
!! TTGATATTAATAGTTATGGA+AGG + chr5_4:13292584-13292603 Msa0838770:three_prime_UTR 20.0%
!!! TTTTTTGTTGGATAATTCAA+TGG + chr5_4:13292618-13292637 Msa0838770:three_prime_UTR 20.0%
! TTGTAGGTTTCGTTATTAAT+CGG + chr5_4:13292005-13292024 Msa0838770:intron 25.0%
!!! AAGTTTTAATTGGCTAATGT+TGG + chr5_4:13292028-13292047 Msa0838770:intron 25.0%
!!! AGTTTTAATTGGCTAATGTT+GGG + chr5_4:13292029-13292048 Msa0838770:intron 25.0%
! TTGTAGGTTTCGTTATTAAT+CGG + chr5_4:13292005-13292024 Msa0838770:intron 25.0%
!!! AAGTTTTAATTGGCTAATGT+TGG + chr5_4:13292028-13292047 Msa0838770:intron 25.0%
!!! AGTTTTAATTGGCTAATGTT+GGG + chr5_4:13292029-13292048 Msa0838770:intron 25.0%
TTGGAAGTCTTCAAAATCAA+AGG + chr5_4:13291377-13291396 Msa0838770:CDS 30.0%
!!! GCTGTTTATTTTATTCATCC+TGG + chr5_4:13291730-13291749 Msa0838770:CDS 30.0%
ATACGATCATGATGAAGATT+TGG + chr5_4:13292158-13292177 Msa0838770:CDS 30.0%
TCTAATCCATAATCCATCAT+TGG - chr5_4:13292192-13292211 None:intergenic 30.0%
ACAGAGCTTTCAAATAGTTA+TGG + chr5_4:13292435-13292454 Msa0838770:three_prime_UTR 30.0%
CTTTCAAATAGTTATGGAGA+TGG + chr5_4:13292441-13292460 Msa0838770:three_prime_UTR 30.0%
!!! TGTTGCCTTTTGTTTTCTTT+AGG + chr5_4:13292516-13292535 Msa0838770:three_prime_UTR 30.0%
CAAGACCTAAAGAAAACAAA+AGG - chr5_4:13292524-13292543 None:intergenic 30.0%
TTGGAAGTCTTCAAAATCAA+AGG + chr5_4:13291377-13291396 Msa0838770:CDS 30.0%
!!! GCTGTTTATTTTATTCATCC+TGG + chr5_4:13291730-13291749 Msa0838770:CDS 30.0%
ATACGATCATGATGAAGATT+TGG + chr5_4:13292158-13292177 Msa0838770:CDS 30.0%
TCTAATCCATAATCCATCAT+TGG - chr5_4:13292192-13292211 None:intergenic 30.0%
ACAGAGCTTTCAAATAGTTA+TGG + chr5_4:13292435-13292454 Msa0838770:three_prime_UTR 30.0%
CTTTCAAATAGTTATGGAGA+TGG + chr5_4:13292441-13292460 Msa0838770:three_prime_UTR 30.0%
!!! TGTTGCCTTTTGTTTTCTTT+AGG + chr5_4:13292516-13292535 Msa0838770:three_prime_UTR 30.0%
CAAGACCTAAAGAAAACAAA+AGG - chr5_4:13292524-13292543 None:intergenic 30.0%
! TATGATTGTGATGGTGAATG+TGG - chr5_4:13291243-13291262 None:intergenic 35.0%
CGTGATGATTATGATTGTGA+TGG - chr5_4:13291252-13291271 None:intergenic 35.0%
TGCGTAGTGATAGAAGAAAA+AGG - chr5_4:13291293-13291312 None:intergenic 35.0%
TTCTTGTTCAAGCTCAGTTA+TGG - chr5_4:13291342-13291361 None:intergenic 35.0%
AGAAGAACTGCTTGAGAAAT+TGG + chr5_4:13291358-13291377 Msa0838770:CDS 35.0%
ATCAAAGGCAAAGATAAGCA+TGG + chr5_4:13291392-13291411 Msa0838770:CDS 35.0%
! GGAAGAATTTTCCGATAATG+CGG - chr5_4:13291430-13291449 None:intergenic 35.0%
ATTATCGGAAAATTCTTCCC+TGG + chr5_4:13291431-13291450 Msa0838770:CDS 35.0%
!!! ATTTTTTGATTCAGCGCGAT+TGG + chr5_4:13291528-13291547 Msa0838770:intron 35.0%
!!! TGAGGTTCTGAAGAAGTTTT+TGG + chr5_4:13291558-13291577 Msa0838770:CDS 35.0%
AATTTCTTCTTCCCTAGCTT+CGG - chr5_4:13291598-13291617 None:intergenic 35.0%
!!! GCTAATGTTGGGTTTTGATT+GGG + chr5_4:13292040-13292059 Msa0838770:intron 35.0%
GTTATCTATGGGAGAATGTT+AGG + chr5_4:13292106-13292125 Msa0838770:CDS 35.0%
!! TATCGTCCAATGATGGATTA+TGG + chr5_4:13292183-13292202 Msa0838770:CDS 35.0%
ATTAGAGAGTGATCATGCTA+GGG + chr5_4:13292206-13292225 Msa0838770:CDS 35.0%
! ATCTCATAGGGTTTAGGATT+TGG + chr5_4:13292285-13292304 Msa0838770:exon 35.0%
! TCTCATAGGGTTTAGGATTT+GGG + chr5_4:13292286-13292305 Msa0838770:exon 35.0%
TCAAATAGTTATGGAGATGG+AGG + chr5_4:13292444-13292463 Msa0838770:three_prime_UTR 35.0%
CAAATAGTTATGGAGATGGA+GGG + chr5_4:13292445-13292464 Msa0838770:three_prime_UTR 35.0%
! TATGATTGTGATGGTGAATG+TGG - chr5_4:13291243-13291262 None:intergenic 35.0%
CGTGATGATTATGATTGTGA+TGG - chr5_4:13291252-13291271 None:intergenic 35.0%
TGCGTAGTGATAGAAGAAAA+AGG - chr5_4:13291293-13291312 None:intergenic 35.0%
TTCTTGTTCAAGCTCAGTTA+TGG - chr5_4:13291342-13291361 None:intergenic 35.0%
AGAAGAACTGCTTGAGAAAT+TGG + chr5_4:13291358-13291377 Msa0838770:CDS 35.0%
ATCAAAGGCAAAGATAAGCA+TGG + chr5_4:13291392-13291411 Msa0838770:CDS 35.0%
! GGAAGAATTTTCCGATAATG+CGG - chr5_4:13291430-13291449 None:intergenic 35.0%
ATTATCGGAAAATTCTTCCC+TGG + chr5_4:13291431-13291450 Msa0838770:CDS 35.0%
!!! ATTTTTTGATTCAGCGCGAT+TGG + chr5_4:13291528-13291547 Msa0838770:intron 35.0%
!!! TGAGGTTCTGAAGAAGTTTT+TGG + chr5_4:13291558-13291577 Msa0838770:CDS 35.0%
AATTTCTTCTTCCCTAGCTT+CGG - chr5_4:13291598-13291617 None:intergenic 35.0%
!!! GCTAATGTTGGGTTTTGATT+GGG + chr5_4:13292040-13292059 Msa0838770:intron 35.0%
GTTATCTATGGGAGAATGTT+AGG + chr5_4:13292106-13292125 Msa0838770:CDS 35.0%
!! TATCGTCCAATGATGGATTA+TGG + chr5_4:13292183-13292202 Msa0838770:CDS 35.0%
ATTAGAGAGTGATCATGCTA+GGG + chr5_4:13292206-13292225 Msa0838770:CDS 35.0%
! ATCTCATAGGGTTTAGGATT+TGG + chr5_4:13292285-13292304 Msa0838770:exon 35.0%
! TCTCATAGGGTTTAGGATTT+GGG + chr5_4:13292286-13292305 Msa0838770:exon 35.0%
TCAAATAGTTATGGAGATGG+AGG + chr5_4:13292444-13292463 Msa0838770:three_prime_UTR 35.0%
CAAATAGTTATGGAGATGGA+GGG + chr5_4:13292445-13292464 Msa0838770:three_prime_UTR 35.0%
ATGGTGAATGTGGATGGATA+GGG - chr5_4:13291233-13291252 None:intergenic 40.0%
! ATTGTGATGGTGAATGTGGA+TGG - chr5_4:13291239-13291258 None:intergenic 40.0%
TTGTTCAAGCTCAGTTATGG+TGG - chr5_4:13291339-13291358 None:intergenic 40.0%
! AGAATTTTCCGATAATGCGG+AGG - chr5_4:13291427-13291446 None:intergenic 40.0%
!!! GGTTCTGAAGAAGTTTTTGG+AGG + chr5_4:13291561-13291580 Msa0838770:CDS 40.0%
!! TGAAGAAGTTTTTGGAGGAG+AGG + chr5_4:13291566-13291585 Msa0838770:CDS 40.0%
ATTTCTTCTTCCCTAGCTTC+GGG - chr5_4:13291597-13291616 None:intergenic 40.0%
!! GGCTAATGTTGGGTTTTGAT+TGG + chr5_4:13292039-13292058 Msa0838770:intron 40.0%
! TCAGCAGAGTTGGTTATCTA+TGG + chr5_4:13292094-13292113 Msa0838770:CDS 40.0%
! CAGCAGAGTTGGTTATCTAT+GGG + chr5_4:13292095-13292114 Msa0838770:CDS 40.0%
ATCTATGGGAGAATGTTAGG+AGG + chr5_4:13292109-13292128 Msa0838770:CDS 40.0%
TCATGATCGTATACGAACTC+CGG - chr5_4:13292150-13292169 None:intergenic 40.0%
!! TTTGGAGTATCGTCCAATGA+TGG + chr5_4:13292176-13292195 Msa0838770:CDS 40.0%
GATTAGAGAGTGATCATGCT+AGG + chr5_4:13292205-13292224 Msa0838770:CDS 40.0%
AGTGATCATGCTAGGGTTTA+TGG + chr5_4:13292213-13292232 Msa0838770:CDS 40.0%
TCCATGAGGTGTATCTCATA+GGG + chr5_4:13292273-13292292 Msa0838770:CDS 40.0%
AGGTGTATCTCATAGGGTTT+AGG + chr5_4:13292279-13292298 Msa0838770:exon 40.0%
ATGGTGAATGTGGATGGATA+GGG - chr5_4:13291233-13291252 None:intergenic 40.0%
! ATTGTGATGGTGAATGTGGA+TGG - chr5_4:13291239-13291258 None:intergenic 40.0%
TTGTTCAAGCTCAGTTATGG+TGG - chr5_4:13291339-13291358 None:intergenic 40.0%
! AGAATTTTCCGATAATGCGG+AGG - chr5_4:13291427-13291446 None:intergenic 40.0%
!!! GGTTCTGAAGAAGTTTTTGG+AGG + chr5_4:13291561-13291580 Msa0838770:CDS 40.0%
!! TGAAGAAGTTTTTGGAGGAG+AGG + chr5_4:13291566-13291585 Msa0838770:CDS 40.0%
ATTTCTTCTTCCCTAGCTTC+GGG - chr5_4:13291597-13291616 None:intergenic 40.0%
!! GGCTAATGTTGGGTTTTGAT+TGG + chr5_4:13292039-13292058 Msa0838770:intron 40.0%
! TCAGCAGAGTTGGTTATCTA+TGG + chr5_4:13292094-13292113 Msa0838770:CDS 40.0%
! CAGCAGAGTTGGTTATCTAT+GGG + chr5_4:13292095-13292114 Msa0838770:CDS 40.0%
ATCTATGGGAGAATGTTAGG+AGG + chr5_4:13292109-13292128 Msa0838770:CDS 40.0%
TCATGATCGTATACGAACTC+CGG - chr5_4:13292150-13292169 None:intergenic 40.0%
!! TTTGGAGTATCGTCCAATGA+TGG + chr5_4:13292176-13292195 Msa0838770:CDS 40.0%
GATTAGAGAGTGATCATGCT+AGG + chr5_4:13292205-13292224 Msa0838770:CDS 40.0%
AGTGATCATGCTAGGGTTTA+TGG + chr5_4:13292213-13292232 Msa0838770:CDS 40.0%
TCCATGAGGTGTATCTCATA+GGG + chr5_4:13292273-13292292 Msa0838770:CDS 40.0%
AGGTGTATCTCATAGGGTTT+AGG + chr5_4:13292279-13292298 Msa0838770:exon 40.0%
GATGGTGAATGTGGATGGAT+AGG - chr5_4:13291234-13291253 None:intergenic 45.0%
! AGAGGATTTTCCCGAAGCTA+GGG + chr5_4:13291584-13291603 Msa0838770:CDS 45.0%
GCTAGGGAAGAAGAAATTCG+CGG + chr5_4:13291600-13291619 Msa0838770:CDS 45.0%
!! GCGGTGTTGTATATTCACAC+CGG + chr5_4:13291619-13291638 Msa0838770:CDS 45.0%
CGATCGGAGAGAAGAAATTC+CGG - chr5_4:13291668-13291687 None:intergenic 45.0%
CGGAATTTCTTCTCTCCGAT+CGG + chr5_4:13291666-13291685 Msa0838770:CDS 45.0%
GAATCGCATCGTAAACCGAT+CGG - chr5_4:13291684-13291703 None:intergenic 45.0%
! ATCGGTTTACGATGCGATTC+CGG + chr5_4:13291684-13291703 Msa0838770:CDS 45.0%
AAAAGGCGAGCTTGTAAACC+AGG - chr5_4:13291751-13291770 None:intergenic 45.0%
! ACCCAGCGTTGAAGAGAAAA+CGG - chr5_4:13291789-13291808 None:intergenic 45.0%
!!! GGGTTTTGATTGGGTGTGAT+AGG + chr5_4:13292049-13292068 Msa0838770:intron 45.0%
TTGGGTGTGATAGGTTGTAC+AGG + chr5_4:13292058-13292077 Msa0838770:intron 45.0%
ATAGGTTGTACAGGAAGCTG+AGG + chr5_4:13292067-13292086 Msa0838770:intron 45.0%
GAATGTTAGGAGGAACGAAG+TGG + chr5_4:13292119-13292138 Msa0838770:CDS 45.0%
CTCATGGAGTAAGTAGTCAC+AGG - chr5_4:13292261-13292280 None:intergenic 45.0%
CTGTGACTACTTACTCCATG+AGG + chr5_4:13292259-13292278 Msa0838770:CDS 45.0%
CTCCATGAGGTGTATCTCAT+AGG + chr5_4:13292272-13292291 Msa0838770:CDS 45.0%
ACCCTATGAGATACACCTCA+TGG - chr5_4:13292277-13292296 None:intergenic 45.0%
! TTTGGGTTCACTGTAGTCGT+TGG + chr5_4:13292303-13292322 Msa0838770:three_prime_UTR 45.0%
GTTCACTGTAGTCGTTGGAT+TGG + chr5_4:13292308-13292327 Msa0838770:three_prime_UTR 45.0%
GATGGTGAATGTGGATGGAT+AGG - chr5_4:13291234-13291253 None:intergenic 45.0%
! AGAGGATTTTCCCGAAGCTA+GGG + chr5_4:13291584-13291603 Msa0838770:CDS 45.0%
GCTAGGGAAGAAGAAATTCG+CGG + chr5_4:13291600-13291619 Msa0838770:CDS 45.0%
!! GCGGTGTTGTATATTCACAC+CGG + chr5_4:13291619-13291638 Msa0838770:CDS 45.0%
CGATCGGAGAGAAGAAATTC+CGG - chr5_4:13291668-13291687 None:intergenic 45.0%
CGGAATTTCTTCTCTCCGAT+CGG + chr5_4:13291666-13291685 Msa0838770:CDS 45.0%
GAATCGCATCGTAAACCGAT+CGG - chr5_4:13291684-13291703 None:intergenic 45.0%
! ATCGGTTTACGATGCGATTC+CGG + chr5_4:13291684-13291703 Msa0838770:CDS 45.0%
AAAAGGCGAGCTTGTAAACC+AGG - chr5_4:13291751-13291770 None:intergenic 45.0%
! ACCCAGCGTTGAAGAGAAAA+CGG - chr5_4:13291789-13291808 None:intergenic 45.0%
!!! GGGTTTTGATTGGGTGTGAT+AGG + chr5_4:13292049-13292068 Msa0838770:intron 45.0%
TTGGGTGTGATAGGTTGTAC+AGG + chr5_4:13292058-13292077 Msa0838770:intron 45.0%
ATAGGTTGTACAGGAAGCTG+AGG + chr5_4:13292067-13292086 Msa0838770:intron 45.0%
GAATGTTAGGAGGAACGAAG+TGG + chr5_4:13292119-13292138 Msa0838770:CDS 45.0%
CTCATGGAGTAAGTAGTCAC+AGG - chr5_4:13292261-13292280 None:intergenic 45.0%
CTGTGACTACTTACTCCATG+AGG + chr5_4:13292259-13292278 Msa0838770:CDS 45.0%
CTCCATGAGGTGTATCTCAT+AGG + chr5_4:13292272-13292291 Msa0838770:CDS 45.0%
ACCCTATGAGATACACCTCA+TGG - chr5_4:13292277-13292296 None:intergenic 45.0%
! TTTGGGTTCACTGTAGTCGT+TGG + chr5_4:13292303-13292322 Msa0838770:three_prime_UTR 45.0%
GTTCACTGTAGTCGTTGGAT+TGG + chr5_4:13292308-13292327 Msa0838770:three_prime_UTR 45.0%
CGATAATGCGGAGGATCTTG+CGG - chr5_4:13291418-13291437 None:intergenic 50.0%
CGCAAGATCCTCCGCATTAT+CGG + chr5_4:13291416-13291435 Msa0838770:CDS 50.0%
! GAGAGGATTTTCCCGAAGCT+AGG + chr5_4:13291583-13291602 Msa0838770:CDS 50.0%
ATATTCACACCGGCGTTCAG+AGG + chr5_4:13291629-13291648 Msa0838770:CDS 50.0%
! CAGAGGAGCGAGAATTTTGC+CGG + chr5_4:13291646-13291665 Msa0838770:CDS 50.0%
AGATTCTCCTTCACGTTCGC+CGG - chr5_4:13291706-13291725 None:intergenic 50.0%
! CGCCTTTTTCTCGCTACGTT+TGG + chr5_4:13291763-13291782 Msa0838770:CDS 50.0%
GGCCAAACGTAGCGAGAAAA+AGG - chr5_4:13291768-13291787 None:intergenic 50.0%
!! GCCGTTTTCTCTTCAACGCT+GGG + chr5_4:13291785-13291804 Msa0838770:CDS 50.0%
CTGAGGTACATCAGCAGAGT+TGG + chr5_4:13292084-13292103 Msa0838770:CDS 50.0%
GAGGAACGAAGTGGAGATTC+CGG + chr5_4:13292128-13292147 Msa0838770:CDS 50.0%
CGATAATGCGGAGGATCTTG+CGG - chr5_4:13291418-13291437 None:intergenic 50.0%
CGCAAGATCCTCCGCATTAT+CGG + chr5_4:13291416-13291435 Msa0838770:CDS 50.0%
! GAGAGGATTTTCCCGAAGCT+AGG + chr5_4:13291583-13291602 Msa0838770:CDS 50.0%
ATATTCACACCGGCGTTCAG+AGG + chr5_4:13291629-13291648 Msa0838770:CDS 50.0%
! CAGAGGAGCGAGAATTTTGC+CGG + chr5_4:13291646-13291665 Msa0838770:CDS 50.0%
AGATTCTCCTTCACGTTCGC+CGG - chr5_4:13291706-13291725 None:intergenic 50.0%
! CGCCTTTTTCTCGCTACGTT+TGG + chr5_4:13291763-13291782 Msa0838770:CDS 50.0%
GGCCAAACGTAGCGAGAAAA+AGG - chr5_4:13291768-13291787 None:intergenic 50.0%
!! GCCGTTTTCTCTTCAACGCT+GGG + chr5_4:13291785-13291804 Msa0838770:CDS 50.0%
CTGAGGTACATCAGCAGAGT+TGG + chr5_4:13292084-13292103 Msa0838770:CDS 50.0%
GAGGAACGAAGTGGAGATTC+CGG + chr5_4:13292128-13292147 Msa0838770:CDS 50.0%
! AGCGCGATTGGTTAGCGTTG+AGG + chr5_4:13291540-13291559 Msa0838770:intron 55.0%
ATTCTCGCTCCTCTGAACGC+CGG - chr5_4:13291641-13291660 None:intergenic 55.0%
!! GGCCGTTTTCTCTTCAACGC+TGG + chr5_4:13291784-13291803 Msa0838770:CDS 55.0%
GTCACAGGCGAATCTAGTGC+AGG - chr5_4:13292246-13292265 None:intergenic 55.0%
! AGCGCGATTGGTTAGCGTTG+AGG + chr5_4:13291540-13291559 Msa0838770:intron 55.0%
ATTCTCGCTCCTCTGAACGC+CGG - chr5_4:13291641-13291660 None:intergenic 55.0%
!! GGCCGTTTTCTCTTCAACGC+TGG + chr5_4:13291784-13291803 Msa0838770:CDS 55.0%
GTCACAGGCGAATCTAGTGC+AGG - chr5_4:13292246-13292265 None:intergenic 55.0%
TGCGATTCCGGCGAACGTGA+AGG + chr5_4:13291696-13291715 Msa0838770:CDS 60.0%
TGCGATTCCGGCGAACGTGA+AGG + chr5_4:13291696-13291715 Msa0838770:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 13291223 13292659 13291223 ID=Msa0838770;Name=Msa0838770
chr5_4 mRNA 13291223 13292659 13291223 ID=Msa0838770-mRNA-1;Parent=Msa0838770;Name=Msa0838770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=106|1|1|1|1|1|3|366|203
chr5_4 exon 13291223 13291452 13291223 ID=Msa0838770-mRNA-1:exon:5938;Parent=Msa0838770-mRNA-1
chr5_4 exon 13291542 13291806 13291542 ID=Msa0838770-mRNA-1:exon:5939;Parent=Msa0838770-mRNA-1
chr5_4 exon 13292071 13292659 13292071 ID=Msa0838770-mRNA-1:exon:5940;Parent=Msa0838770-mRNA-1
chr5_4 five_prime_UTR 13291223 13291328 13291223 ID=Msa0838770-mRNA-1:five_prime_utr;Parent=Msa0838770-mRNA-1
chr5_4 CDS 13291329 13291452 13291329 ID=Msa0838770-mRNA-1:cds;Parent=Msa0838770-mRNA-1
chr5_4 CDS 13291542 13291806 13291542 ID=Msa0838770-mRNA-1:cds;Parent=Msa0838770-mRNA-1
chr5_4 CDS 13292071 13292293 13292071 ID=Msa0838770-mRNA-1:cds;Parent=Msa0838770-mRNA-1
chr5_4 three_prime_UTR 13292294 13292659 13292294 ID=Msa0838770-mRNA-1:three_prime_utr;Parent=Msa0838770-mRNA-1
Gene Sequence

>Msa0838770

ATGAGCACCACCATAACTGAGCTTGAACAAGAAGAACTGCTTGAGAAATTGGAAGTCTTCAAAATCAAAGGCAAAGATAAGCATGGCCGCAAGATCCTCCGCATTATCGGAAAATTCTTCCCTGCGCGATTGGTTAGCGTTGAGGTTCTGAAGAAGTTTTTGGAGGAGAGGATTTTCCCGAAGCTAGGGAAGAAGAAATTCGCGGTGTTGTATATTCACACCGGCGTTCAGAGGAGCGAGAATTTTGCCGGAATTTCTTCTCTCCGATCGGTTTACGATGCGATTCCGGCGAACGTGAAGGAGAATCTAGAAGCTGTTTATTTTATTCATCCTGGTTTACAAGCTCGCCTTTTTCTCGCTACGTTTGGCCGTTTTCTCTTCAACGCTGGGTTGTACAGGAAGCTGAGGTACATCAGCAGAGTTGGTTATCTATGGGAGAATGTTAGGAGGAACGAAGTGGAGATTCCGGAGTTCGTATACGATCATGATGAAGATTTGGAGTATCGTCCAATGATGGATTATGGATTAGAGAGTGATCATGCTAGGGTTTATGGTACTGCTCCTGCACTAGATTCGCCTGTGACTACTTACTCCATGAGGTGTATCTCATAG
Protein sequence

>Msa0838770

MSTTITELEQEELLEKLEVFKIKGKDKHGRKILRIIGKFFPARLVSVEVLKKFLEERIFPKLGKKKFAVLYIHTGVQRSENFAGISSLRSVYDAIPANVKENLEAVYFIHPGLQARLFLATFGRFLFNAGLYRKLRYISRVGYLWENVRRNEVEIPEFVYDHDEDLEYRPMMDYGLESDHARVYGTAPALDSPVTTYSMRCIS*