AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0712930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712930 MtrunA17_Chr5g0406301 98.522 203 3 0 1 203 1 203 4.48e-149 412
Msa0712930 MtrunA17_Chr7g0256341 45.930 172 93 0 14 185 51 222 7.23e-57 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712930 AT4G35750.1 75.369 203 49 1 1 203 1 202 1.96e-117 332
Msa0712930 AT3G10210.1 47.590 166 87 0 14 179 49 214 8.59e-54 172

Find 66 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATTGTGATGGTGAATT+TGG 0.185149 5_1:-4834388 None:intergenic
AGTGATCATGCTAGGGTTTA+TGG 0.201786 5_1:+4835359 Msa0712930:CDS
ATCTCATAGGGTTTAGGATT+TGG 0.237359 5_1:+4835431 Msa0712930:exon
AGAAGAACTGCTTGAGAAAT+TGG 0.241731 5_1:+4834504 Msa0712930:CDS
TCTCATAGGGTTTAGGATTT+GGG 0.254159 5_1:+4835432 Msa0712930:exon
TCAGCAGAGTTGGTTATCTA+TGG 0.276388 5_1:+4835240 Msa0712930:CDS
GTTATCTATGGGAGAATGTT+AGG 0.282831 5_1:+4835252 Msa0712930:CDS
CATCTTGATATTAATAGTTA+TGG 0.321706 5_1:+4835721 Msa0712930:three_prime_UTR
TATCGTCCAATGATGGATTA+TGG 0.323869 5_1:+4835329 Msa0712930:CDS
GGCCAAACGTAGCGAGAAAA+AGG 0.325265 5_1:-4834911 None:intergenic
AGGTGTATCTCATAGGGTTT+AGG 0.338603 5_1:+4835425 Msa0712930:exon
CGCAAGATTCTCCGCATTAT+CGG 0.351043 5_1:+4834562 Msa0712930:CDS
CTCCATGAGGTGTATCTCAT+AGG 0.352468 5_1:+4835418 Msa0712930:CDS
AATTTCTTCTTCCCTAGCTT+CGG 0.354119 5_1:-4834741 None:intergenic
ATCGGTTTACGATGCGATTC+CGG 0.372506 5_1:+4834830 Msa0712930:CDS
ACAGAGCTTTCAAATAGTTA+TGG 0.379055 5_1:+4835582 Msa0712930:three_prime_UTR
ATACGATCATGATGAAGATT+TGG 0.383633 5_1:+4835304 Msa0712930:CDS
TTCTTGTTCAAGCTCAGTTA+TGG 0.385949 5_1:-4834485 None:intergenic
ACATTATATCTCCATAACTA+TGG 0.395608 5_1:-4835609 None:intergenic
TGCGTAGTGATAGAAGAAAA+AGG 0.406840 5_1:-4834438 None:intergenic
GAGGAACGAAGTGGAGATTC+CGG 0.411726 5_1:+4835274 Msa0712930:CDS
ATTTCTTCTTCCCTAGCTTC+GGG 0.419960 5_1:-4834740 None:intergenic
CGGAATTTCTTCTCTCCGAT+CGG 0.427792 5_1:+4834812 Msa0712930:CDS
CGATCGGAGAGAAGAAATTC+CGG 0.432814 5_1:-4834811 None:intergenic
AGATTCTCCTTCACGTTCGC+CGG 0.437993 5_1:-4834849 None:intergenic
TCTAATCCATAATCCATCAT+TGG 0.455280 5_1:-4835335 None:intergenic
GTTATGGAGGGCCATAGTTA+TGG 0.461359 5_1:+4835598 Msa0712930:three_prime_UTR
TTTGGAGTATCGTCCAATGA+TGG 0.485084 5_1:+4835322 Msa0712930:CDS
GTTCACTGTAGTCGTTGGAT+TGG 0.491143 5_1:+4835454 Msa0712930:three_prime_UTR
TTTGGGTTCACTGTAGTCGT+TGG 0.495682 5_1:+4835449 Msa0712930:three_prime_UTR
TTGGGTGTGATAGGTTGTAC+AGG 0.501143 5_1:+4835204 Msa0712930:intron
GATGGTGAATTTGGATGGAT+AGG 0.507550 5_1:-4834379 None:intergenic
GATTAGAGAGTGATCATGCT+AGG 0.515483 5_1:+4835351 Msa0712930:CDS
TCCATGAGGTGTATCTCATA+GGG 0.515766 5_1:+4835419 Msa0712930:CDS
CTGAGGTACATCAGCAGAGT+TGG 0.517318 5_1:+4835230 Msa0712930:CDS
ACCCAGCGTTGAAGAGAAAA+CGG 0.517837 5_1:-4834932 None:intergenic
CAAGACCTAAAGAAAACAAA+AGG 0.523861 5_1:-4835662 None:intergenic
TTGGAAGTCTTCAAAATCAA+AGG 0.533957 5_1:+4834523 Msa0712930:CDS
AGCGCGATTGGTTAGCGTTG+AGG 0.536404 5_1:+4834686 Msa0712930:intron
ATGGTGAATTTGGATGGATA+GGG 0.536686 5_1:-4834378 None:intergenic
TTGTTCAAGCTCAGTTATGG+TGG 0.540444 5_1:-4834482 None:intergenic
CAGCAGAGTTGGTTATCTAT+GGG 0.551346 5_1:+4835241 Msa0712930:CDS
GAGCTTTCAAATAGTTATGG+AGG 0.553818 5_1:+4835585 Msa0712930:three_prime_UTR
GTCACAGGCGAATCTAGTGC+AGG 0.559387 5_1:-4835389 None:intergenic
AAAAGGCGAGCTTGTAAACC+AGG 0.560268 5_1:-4834894 None:intergenic
ATTGTGATGGTGAATTTGGA+TGG 0.571335 5_1:-4834384 None:intergenic
ATTATCGGAAAATTCTTCCC+TGG 0.573008 5_1:+4834577 Msa0712930:CDS
TTGATATTAATAGTTATGGA+AGG 0.590651 5_1:+4835725 Msa0712930:three_prime_UTR
TGCGATTCCGGCGAACGTGA+AGG 0.593355 5_1:+4834842 Msa0712930:CDS
AGCTTTCAAATAGTTATGGA+GGG 0.593526 5_1:+4835586 Msa0712930:three_prime_UTR
ATTAGAGAGTGATCATGCTA+GGG 0.594329 5_1:+4835352 Msa0712930:CDS
CTCATGGAGTAAGTAGTCAC+AGG 0.597762 5_1:-4835404 None:intergenic
ACCCTATGAGATACACCTCA+TGG 0.597933 5_1:-4835420 None:intergenic
TCATGATCGTATACGAACTC+CGG 0.600493 5_1:-4835293 None:intergenic
GAATGTTAGGAGGAACGAAG+TGG 0.610161 5_1:+4835265 Msa0712930:CDS
CGATAATGCGGAGAATCTTG+CGG 0.614669 5_1:-4834561 None:intergenic
CGTGATGATTATGATTGTGA+TGG 0.623135 5_1:-4834397 None:intergenic
ATATTCACACCGGCGTTCAG+AGG 0.623653 5_1:+4834775 Msa0712930:CDS
ATCAAAGGCAAAGATAAGCA+TGG 0.637350 5_1:+4834538 Msa0712930:CDS
GAATCGCATCGTAAACCGAT+CGG 0.641309 5_1:-4834827 None:intergenic
ATCTATGGGAGAATGTTAGG+AGG 0.655849 5_1:+4835255 Msa0712930:CDS
ATAGGTTGTACAGGAAGCTG+AGG 0.667654 5_1:+4835213 Msa0712930:intron
ATTCTCGCTCCTCTGAACGC+CGG 0.670180 5_1:-4834784 None:intergenic
GCTAGGGAAGAAGAAATTCG+CGG 0.672425 5_1:+4834746 Msa0712930:CDS
CTGTGACTACTTACTCCATG+AGG 0.695115 5_1:+4835405 Msa0712930:CDS
GCGGTGTTGTATATTCACAC+CGG 0.721011 5_1:+4834765 Msa0712930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAATCACATATATAA+CGG - chr5_1:4834628-4834647 None:intergenic 15.0%
!!! GATATTTTCATTTTTTTTGT+TGG + chr5_1:4835747-4835766 Msa0712930:three_prime_UTR 15.0%
!! AACAAAAATCACATATATAA+CGG - chr5_1:4834628-4834647 None:intergenic 15.0%
!!! GATATTTTCATTTTTTTTGT+TGG + chr5_1:4835747-4835766 Msa0712930:three_prime_UTR 15.0%
!!! AACTGAAATAATTTTTACCA+GGG - chr5_1:4834597-4834616 None:intergenic 20.0%
!!! AAACTGAAATAATTTTTACC+AGG - chr5_1:4834598-4834617 None:intergenic 20.0%
!!! GATTAATTTAAAGCTGATTT+AGG - chr5_1:4835015-4835034 None:intergenic 20.0%
!! AAATTAATGAAGTTGTTTGT+AGG + chr5_1:4835135-4835154 Msa0712930:intron 20.0%
!!! TATTAATCGGAAGTTTTAAT+TGG + chr5_1:4835164-4835183 Msa0712930:intron 20.0%
!! CATCTTGATATTAATAGTTA+TGG + chr5_1:4835721-4835740 Msa0712930:three_prime_UTR 20.0%
!! TTGATATTAATAGTTATGGA+AGG + chr5_1:4835725-4835744 Msa0712930:three_prime_UTR 20.0%
!!! TTTTTTGTTGGATAATTCAA+TGG + chr5_1:4835759-4835778 Msa0712930:three_prime_UTR 20.0%
!!! AACTGAAATAATTTTTACCA+GGG - chr5_1:4834597-4834616 None:intergenic 20.0%
!!! AAACTGAAATAATTTTTACC+AGG - chr5_1:4834598-4834617 None:intergenic 20.0%
!!! GATTAATTTAAAGCTGATTT+AGG - chr5_1:4835015-4835034 None:intergenic 20.0%
!! AAATTAATGAAGTTGTTTGT+AGG + chr5_1:4835135-4835154 Msa0712930:intron 20.0%
!!! TATTAATCGGAAGTTTTAAT+TGG + chr5_1:4835164-4835183 Msa0712930:intron 20.0%
!! CATCTTGATATTAATAGTTA+TGG + chr5_1:4835721-4835740 Msa0712930:three_prime_UTR 20.0%
!! TTGATATTAATAGTTATGGA+AGG + chr5_1:4835725-4835744 Msa0712930:three_prime_UTR 20.0%
!!! TTTTTTGTTGGATAATTCAA+TGG + chr5_1:4835759-4835778 Msa0712930:three_prime_UTR 20.0%
! TTGTAGGTTTCGTTATTAAT+CGG + chr5_1:4835151-4835170 Msa0712930:intron 25.0%
!!! AAGTTTTAATTGGCTAATGT+TGG + chr5_1:4835174-4835193 Msa0712930:intron 25.0%
!!! AGTTTTAATTGGCTAATGTT+GGG + chr5_1:4835175-4835194 Msa0712930:intron 25.0%
! ACATTATATCTCCATAACTA+TGG - chr5_1:4835612-4835631 None:intergenic 25.0%
! TTGTAGGTTTCGTTATTAAT+CGG + chr5_1:4835151-4835170 Msa0712930:intron 25.0%
!!! AAGTTTTAATTGGCTAATGT+TGG + chr5_1:4835174-4835193 Msa0712930:intron 25.0%
!!! AGTTTTAATTGGCTAATGTT+GGG + chr5_1:4835175-4835194 Msa0712930:intron 25.0%
! ACATTATATCTCCATAACTA+TGG - chr5_1:4835612-4835631 None:intergenic 25.0%
! TATGATTGTGATGGTGAATT+TGG - chr5_1:4834391-4834410 None:intergenic 30.0%
TTGGAAGTCTTCAAAATCAA+AGG + chr5_1:4834523-4834542 Msa0712930:CDS 30.0%
!!! GCTGTTTATTTTATTCATCC+TGG + chr5_1:4834876-4834895 Msa0712930:CDS 30.0%
ATACGATCATGATGAAGATT+TGG + chr5_1:4835304-4835323 Msa0712930:CDS 30.0%
TCTAATCCATAATCCATCAT+TGG - chr5_1:4835338-4835357 None:intergenic 30.0%
ACAGAGCTTTCAAATAGTTA+TGG + chr5_1:4835582-4835601 Msa0712930:three_prime_UTR 30.0%
AGCTTTCAAATAGTTATGGA+GGG + chr5_1:4835586-4835605 Msa0712930:three_prime_UTR 30.0%
!!! TGTTGCCTTTTGTTTTCTTT+AGG + chr5_1:4835657-4835676 Msa0712930:three_prime_UTR 30.0%
CAAGACCTAAAGAAAACAAA+AGG - chr5_1:4835665-4835684 None:intergenic 30.0%
! TATGATTGTGATGGTGAATT+TGG - chr5_1:4834391-4834410 None:intergenic 30.0%
TTGGAAGTCTTCAAAATCAA+AGG + chr5_1:4834523-4834542 Msa0712930:CDS 30.0%
!!! GCTGTTTATTTTATTCATCC+TGG + chr5_1:4834876-4834895 Msa0712930:CDS 30.0%
ATACGATCATGATGAAGATT+TGG + chr5_1:4835304-4835323 Msa0712930:CDS 30.0%
TCTAATCCATAATCCATCAT+TGG - chr5_1:4835338-4835357 None:intergenic 30.0%
ACAGAGCTTTCAAATAGTTA+TGG + chr5_1:4835582-4835601 Msa0712930:three_prime_UTR 30.0%
AGCTTTCAAATAGTTATGGA+GGG + chr5_1:4835586-4835605 Msa0712930:three_prime_UTR 30.0%
!!! TGTTGCCTTTTGTTTTCTTT+AGG + chr5_1:4835657-4835676 Msa0712930:three_prime_UTR 30.0%
CAAGACCTAAAGAAAACAAA+AGG - chr5_1:4835665-4835684 None:intergenic 30.0%
ATGGTGAATTTGGATGGATA+GGG - chr5_1:4834381-4834400 None:intergenic 35.0%
! ATTGTGATGGTGAATTTGGA+TGG - chr5_1:4834387-4834406 None:intergenic 35.0%
CGTGATGATTATGATTGTGA+TGG - chr5_1:4834400-4834419 None:intergenic 35.0%
TGCGTAGTGATAGAAGAAAA+AGG - chr5_1:4834441-4834460 None:intergenic 35.0%
TTCTTGTTCAAGCTCAGTTA+TGG - chr5_1:4834488-4834507 None:intergenic 35.0%
AGAAGAACTGCTTGAGAAAT+TGG + chr5_1:4834504-4834523 Msa0712930:CDS 35.0%
ATCAAAGGCAAAGATAAGCA+TGG + chr5_1:4834538-4834557 Msa0712930:CDS 35.0%
! GGAAGAATTTTCCGATAATG+CGG - chr5_1:4834576-4834595 None:intergenic 35.0%
ATTATCGGAAAATTCTTCCC+TGG + chr5_1:4834577-4834596 Msa0712930:CDS 35.0%
!!! ATTTTTTGATTCAGCGCGAT+TGG + chr5_1:4834674-4834693 Msa0712930:intron 35.0%
!!! TGAGGTTCTGAAGAAGTTTT+TGG + chr5_1:4834704-4834723 Msa0712930:CDS 35.0%
AATTTCTTCTTCCCTAGCTT+CGG - chr5_1:4834744-4834763 None:intergenic 35.0%
!!! GCTAATGTTGGGTTTTGATT+GGG + chr5_1:4835186-4835205 Msa0712930:intron 35.0%
GTTATCTATGGGAGAATGTT+AGG + chr5_1:4835252-4835271 Msa0712930:CDS 35.0%
!! TATCGTCCAATGATGGATTA+TGG + chr5_1:4835329-4835348 Msa0712930:CDS 35.0%
ATTAGAGAGTGATCATGCTA+GGG + chr5_1:4835352-4835371 Msa0712930:CDS 35.0%
! ATCTCATAGGGTTTAGGATT+TGG + chr5_1:4835431-4835450 Msa0712930:exon 35.0%
! TCTCATAGGGTTTAGGATTT+GGG + chr5_1:4835432-4835451 Msa0712930:exon 35.0%
GAGCTTTCAAATAGTTATGG+AGG + chr5_1:4835585-4835604 Msa0712930:three_prime_UTR 35.0%
ATGGTGAATTTGGATGGATA+GGG - chr5_1:4834381-4834400 None:intergenic 35.0%
! ATTGTGATGGTGAATTTGGA+TGG - chr5_1:4834387-4834406 None:intergenic 35.0%
CGTGATGATTATGATTGTGA+TGG - chr5_1:4834400-4834419 None:intergenic 35.0%
TGCGTAGTGATAGAAGAAAA+AGG - chr5_1:4834441-4834460 None:intergenic 35.0%
TTCTTGTTCAAGCTCAGTTA+TGG - chr5_1:4834488-4834507 None:intergenic 35.0%
AGAAGAACTGCTTGAGAAAT+TGG + chr5_1:4834504-4834523 Msa0712930:CDS 35.0%
ATCAAAGGCAAAGATAAGCA+TGG + chr5_1:4834538-4834557 Msa0712930:CDS 35.0%
! GGAAGAATTTTCCGATAATG+CGG - chr5_1:4834576-4834595 None:intergenic 35.0%
ATTATCGGAAAATTCTTCCC+TGG + chr5_1:4834577-4834596 Msa0712930:CDS 35.0%
!!! ATTTTTTGATTCAGCGCGAT+TGG + chr5_1:4834674-4834693 Msa0712930:intron 35.0%
!!! TGAGGTTCTGAAGAAGTTTT+TGG + chr5_1:4834704-4834723 Msa0712930:CDS 35.0%
AATTTCTTCTTCCCTAGCTT+CGG - chr5_1:4834744-4834763 None:intergenic 35.0%
!!! GCTAATGTTGGGTTTTGATT+GGG + chr5_1:4835186-4835205 Msa0712930:intron 35.0%
GTTATCTATGGGAGAATGTT+AGG + chr5_1:4835252-4835271 Msa0712930:CDS 35.0%
!! TATCGTCCAATGATGGATTA+TGG + chr5_1:4835329-4835348 Msa0712930:CDS 35.0%
ATTAGAGAGTGATCATGCTA+GGG + chr5_1:4835352-4835371 Msa0712930:CDS 35.0%
! ATCTCATAGGGTTTAGGATT+TGG + chr5_1:4835431-4835450 Msa0712930:exon 35.0%
! TCTCATAGGGTTTAGGATTT+GGG + chr5_1:4835432-4835451 Msa0712930:exon 35.0%
GAGCTTTCAAATAGTTATGG+AGG + chr5_1:4835585-4835604 Msa0712930:three_prime_UTR 35.0%
GATGGTGAATTTGGATGGAT+AGG - chr5_1:4834382-4834401 None:intergenic 40.0%
TTGTTCAAGCTCAGTTATGG+TGG - chr5_1:4834485-4834504 None:intergenic 40.0%
!!! GGTTCTGAAGAAGTTTTTGG+AGG + chr5_1:4834707-4834726 Msa0712930:CDS 40.0%
!! TGAAGAAGTTTTTGGAGGAG+AGG + chr5_1:4834712-4834731 Msa0712930:CDS 40.0%
ATTTCTTCTTCCCTAGCTTC+GGG - chr5_1:4834743-4834762 None:intergenic 40.0%
!! GGCTAATGTTGGGTTTTGAT+TGG + chr5_1:4835185-4835204 Msa0712930:intron 40.0%
! TCAGCAGAGTTGGTTATCTA+TGG + chr5_1:4835240-4835259 Msa0712930:CDS 40.0%
! CAGCAGAGTTGGTTATCTAT+GGG + chr5_1:4835241-4835260 Msa0712930:CDS 40.0%
ATCTATGGGAGAATGTTAGG+AGG + chr5_1:4835255-4835274 Msa0712930:CDS 40.0%
TCATGATCGTATACGAACTC+CGG - chr5_1:4835296-4835315 None:intergenic 40.0%
!! TTTGGAGTATCGTCCAATGA+TGG + chr5_1:4835322-4835341 Msa0712930:CDS 40.0%
GATTAGAGAGTGATCATGCT+AGG + chr5_1:4835351-4835370 Msa0712930:CDS 40.0%
AGTGATCATGCTAGGGTTTA+TGG + chr5_1:4835359-4835378 Msa0712930:CDS 40.0%
TCCATGAGGTGTATCTCATA+GGG + chr5_1:4835419-4835438 Msa0712930:CDS 40.0%
AGGTGTATCTCATAGGGTTT+AGG + chr5_1:4835425-4835444 Msa0712930:exon 40.0%
GATGGTGAATTTGGATGGAT+AGG - chr5_1:4834382-4834401 None:intergenic 40.0%
TTGTTCAAGCTCAGTTATGG+TGG - chr5_1:4834485-4834504 None:intergenic 40.0%
!!! GGTTCTGAAGAAGTTTTTGG+AGG + chr5_1:4834707-4834726 Msa0712930:CDS 40.0%
!! TGAAGAAGTTTTTGGAGGAG+AGG + chr5_1:4834712-4834731 Msa0712930:CDS 40.0%
ATTTCTTCTTCCCTAGCTTC+GGG - chr5_1:4834743-4834762 None:intergenic 40.0%
!! GGCTAATGTTGGGTTTTGAT+TGG + chr5_1:4835185-4835204 Msa0712930:intron 40.0%
! TCAGCAGAGTTGGTTATCTA+TGG + chr5_1:4835240-4835259 Msa0712930:CDS 40.0%
! CAGCAGAGTTGGTTATCTAT+GGG + chr5_1:4835241-4835260 Msa0712930:CDS 40.0%
ATCTATGGGAGAATGTTAGG+AGG + chr5_1:4835255-4835274 Msa0712930:CDS 40.0%
TCATGATCGTATACGAACTC+CGG - chr5_1:4835296-4835315 None:intergenic 40.0%
!! TTTGGAGTATCGTCCAATGA+TGG + chr5_1:4835322-4835341 Msa0712930:CDS 40.0%
GATTAGAGAGTGATCATGCT+AGG + chr5_1:4835351-4835370 Msa0712930:CDS 40.0%
AGTGATCATGCTAGGGTTTA+TGG + chr5_1:4835359-4835378 Msa0712930:CDS 40.0%
TCCATGAGGTGTATCTCATA+GGG + chr5_1:4835419-4835438 Msa0712930:CDS 40.0%
AGGTGTATCTCATAGGGTTT+AGG + chr5_1:4835425-4835444 Msa0712930:exon 40.0%
CGATAATGCGGAGAATCTTG+CGG - chr5_1:4834564-4834583 None:intergenic 45.0%
CGCAAGATTCTCCGCATTAT+CGG + chr5_1:4834562-4834581 Msa0712930:CDS 45.0%
! AGAGGATTTTCCCGAAGCTA+GGG + chr5_1:4834730-4834749 Msa0712930:CDS 45.0%
GCTAGGGAAGAAGAAATTCG+CGG + chr5_1:4834746-4834765 Msa0712930:CDS 45.0%
!! GCGGTGTTGTATATTCACAC+CGG + chr5_1:4834765-4834784 Msa0712930:CDS 45.0%
CGATCGGAGAGAAGAAATTC+CGG - chr5_1:4834814-4834833 None:intergenic 45.0%
CGGAATTTCTTCTCTCCGAT+CGG + chr5_1:4834812-4834831 Msa0712930:CDS 45.0%
GAATCGCATCGTAAACCGAT+CGG - chr5_1:4834830-4834849 None:intergenic 45.0%
! ATCGGTTTACGATGCGATTC+CGG + chr5_1:4834830-4834849 Msa0712930:CDS 45.0%
AAAAGGCGAGCTTGTAAACC+AGG - chr5_1:4834897-4834916 None:intergenic 45.0%
! ACCCAGCGTTGAAGAGAAAA+CGG - chr5_1:4834935-4834954 None:intergenic 45.0%
!!! GGGTTTTGATTGGGTGTGAT+AGG + chr5_1:4835195-4835214 Msa0712930:intron 45.0%
TTGGGTGTGATAGGTTGTAC+AGG + chr5_1:4835204-4835223 Msa0712930:intron 45.0%
ATAGGTTGTACAGGAAGCTG+AGG + chr5_1:4835213-4835232 Msa0712930:intron 45.0%
GAATGTTAGGAGGAACGAAG+TGG + chr5_1:4835265-4835284 Msa0712930:CDS 45.0%
CTCATGGAGTAAGTAGTCAC+AGG - chr5_1:4835407-4835426 None:intergenic 45.0%
CTGTGACTACTTACTCCATG+AGG + chr5_1:4835405-4835424 Msa0712930:CDS 45.0%
CTCCATGAGGTGTATCTCAT+AGG + chr5_1:4835418-4835437 Msa0712930:CDS 45.0%
ACCCTATGAGATACACCTCA+TGG - chr5_1:4835423-4835442 None:intergenic 45.0%
! TTTGGGTTCACTGTAGTCGT+TGG + chr5_1:4835449-4835468 Msa0712930:three_prime_UTR 45.0%
GTTCACTGTAGTCGTTGGAT+TGG + chr5_1:4835454-4835473 Msa0712930:three_prime_UTR 45.0%
GTTATGGAGGGCCATAGTTA+TGG + chr5_1:4835598-4835617 Msa0712930:three_prime_UTR 45.0%
CGATAATGCGGAGAATCTTG+CGG - chr5_1:4834564-4834583 None:intergenic 45.0%
CGCAAGATTCTCCGCATTAT+CGG + chr5_1:4834562-4834581 Msa0712930:CDS 45.0%
! AGAGGATTTTCCCGAAGCTA+GGG + chr5_1:4834730-4834749 Msa0712930:CDS 45.0%
GCTAGGGAAGAAGAAATTCG+CGG + chr5_1:4834746-4834765 Msa0712930:CDS 45.0%
!! GCGGTGTTGTATATTCACAC+CGG + chr5_1:4834765-4834784 Msa0712930:CDS 45.0%
CGATCGGAGAGAAGAAATTC+CGG - chr5_1:4834814-4834833 None:intergenic 45.0%
CGGAATTTCTTCTCTCCGAT+CGG + chr5_1:4834812-4834831 Msa0712930:CDS 45.0%
GAATCGCATCGTAAACCGAT+CGG - chr5_1:4834830-4834849 None:intergenic 45.0%
! ATCGGTTTACGATGCGATTC+CGG + chr5_1:4834830-4834849 Msa0712930:CDS 45.0%
AAAAGGCGAGCTTGTAAACC+AGG - chr5_1:4834897-4834916 None:intergenic 45.0%
! ACCCAGCGTTGAAGAGAAAA+CGG - chr5_1:4834935-4834954 None:intergenic 45.0%
!!! GGGTTTTGATTGGGTGTGAT+AGG + chr5_1:4835195-4835214 Msa0712930:intron 45.0%
TTGGGTGTGATAGGTTGTAC+AGG + chr5_1:4835204-4835223 Msa0712930:intron 45.0%
ATAGGTTGTACAGGAAGCTG+AGG + chr5_1:4835213-4835232 Msa0712930:intron 45.0%
GAATGTTAGGAGGAACGAAG+TGG + chr5_1:4835265-4835284 Msa0712930:CDS 45.0%
CTCATGGAGTAAGTAGTCAC+AGG - chr5_1:4835407-4835426 None:intergenic 45.0%
CTGTGACTACTTACTCCATG+AGG + chr5_1:4835405-4835424 Msa0712930:CDS 45.0%
CTCCATGAGGTGTATCTCAT+AGG + chr5_1:4835418-4835437 Msa0712930:CDS 45.0%
ACCCTATGAGATACACCTCA+TGG - chr5_1:4835423-4835442 None:intergenic 45.0%
! TTTGGGTTCACTGTAGTCGT+TGG + chr5_1:4835449-4835468 Msa0712930:three_prime_UTR 45.0%
GTTCACTGTAGTCGTTGGAT+TGG + chr5_1:4835454-4835473 Msa0712930:three_prime_UTR 45.0%
GTTATGGAGGGCCATAGTTA+TGG + chr5_1:4835598-4835617 Msa0712930:three_prime_UTR 45.0%
! GAGAGGATTTTCCCGAAGCT+AGG + chr5_1:4834729-4834748 Msa0712930:CDS 50.0%
ATATTCACACCGGCGTTCAG+AGG + chr5_1:4834775-4834794 Msa0712930:CDS 50.0%
! CAGAGGAGCGAGAATTTTGC+CGG + chr5_1:4834792-4834811 Msa0712930:CDS 50.0%
AGATTCTCCTTCACGTTCGC+CGG - chr5_1:4834852-4834871 None:intergenic 50.0%
! CGCCTTTTTCTCGCTACGTT+TGG + chr5_1:4834909-4834928 Msa0712930:CDS 50.0%
GGCCAAACGTAGCGAGAAAA+AGG - chr5_1:4834914-4834933 None:intergenic 50.0%
!! GCCGTTTTCTCTTCAACGCT+GGG + chr5_1:4834931-4834950 Msa0712930:CDS 50.0%
CTGAGGTACATCAGCAGAGT+TGG + chr5_1:4835230-4835249 Msa0712930:CDS 50.0%
GAGGAACGAAGTGGAGATTC+CGG + chr5_1:4835274-4835293 Msa0712930:CDS 50.0%
! GAGAGGATTTTCCCGAAGCT+AGG + chr5_1:4834729-4834748 Msa0712930:CDS 50.0%
ATATTCACACCGGCGTTCAG+AGG + chr5_1:4834775-4834794 Msa0712930:CDS 50.0%
! CAGAGGAGCGAGAATTTTGC+CGG + chr5_1:4834792-4834811 Msa0712930:CDS 50.0%
AGATTCTCCTTCACGTTCGC+CGG - chr5_1:4834852-4834871 None:intergenic 50.0%
! CGCCTTTTTCTCGCTACGTT+TGG + chr5_1:4834909-4834928 Msa0712930:CDS 50.0%
GGCCAAACGTAGCGAGAAAA+AGG - chr5_1:4834914-4834933 None:intergenic 50.0%
!! GCCGTTTTCTCTTCAACGCT+GGG + chr5_1:4834931-4834950 Msa0712930:CDS 50.0%
CTGAGGTACATCAGCAGAGT+TGG + chr5_1:4835230-4835249 Msa0712930:CDS 50.0%
GAGGAACGAAGTGGAGATTC+CGG + chr5_1:4835274-4835293 Msa0712930:CDS 50.0%
! AGCGCGATTGGTTAGCGTTG+AGG + chr5_1:4834686-4834705 Msa0712930:intron 55.0%
ATTCTCGCTCCTCTGAACGC+CGG - chr5_1:4834787-4834806 None:intergenic 55.0%
!! GGCCGTTTTCTCTTCAACGC+TGG + chr5_1:4834930-4834949 Msa0712930:CDS 55.0%
GTCACAGGCGAATCTAGTGC+AGG - chr5_1:4835392-4835411 None:intergenic 55.0%
! AGCGCGATTGGTTAGCGTTG+AGG + chr5_1:4834686-4834705 Msa0712930:intron 55.0%
ATTCTCGCTCCTCTGAACGC+CGG - chr5_1:4834787-4834806 None:intergenic 55.0%
!! GGCCGTTTTCTCTTCAACGC+TGG + chr5_1:4834930-4834949 Msa0712930:CDS 55.0%
GTCACAGGCGAATCTAGTGC+AGG - chr5_1:4835392-4835411 None:intergenic 55.0%
TGCGATTCCGGCGAACGTGA+AGG + chr5_1:4834842-4834861 Msa0712930:CDS 60.0%
TGCGATTCCGGCGAACGTGA+AGG + chr5_1:4834842-4834861 Msa0712930:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 4834371 4835800 4834371 ID=Msa0712930;Name=Msa0712930
chr5_1 mRNA 4834371 4835800 4834371 ID=Msa0712930-mRNA-1;Parent=Msa0712930;Name=Msa0712930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=104|1|1|1|1|1|3|361|203
chr5_1 exon 4834371 4834598 4834371 ID=Msa0712930-mRNA-1:exon:2014;Parent=Msa0712930-mRNA-1
chr5_1 exon 4834688 4834952 4834688 ID=Msa0712930-mRNA-1:exon:2015;Parent=Msa0712930-mRNA-1
chr5_1 exon 4835217 4835800 4835217 ID=Msa0712930-mRNA-1:exon:2016;Parent=Msa0712930-mRNA-1
chr5_1 five_prime_UTR 4834371 4834474 4834371 ID=Msa0712930-mRNA-1:five_prime_utr;Parent=Msa0712930-mRNA-1
chr5_1 CDS 4834475 4834598 4834475 ID=Msa0712930-mRNA-1:cds;Parent=Msa0712930-mRNA-1
chr5_1 CDS 4834688 4834952 4834688 ID=Msa0712930-mRNA-1:cds;Parent=Msa0712930-mRNA-1
chr5_1 CDS 4835217 4835439 4835217 ID=Msa0712930-mRNA-1:cds;Parent=Msa0712930-mRNA-1
chr5_1 three_prime_UTR 4835440 4835800 4835440 ID=Msa0712930-mRNA-1:three_prime_utr;Parent=Msa0712930-mRNA-1
Gene Sequence

>Msa0712930

ATGAGCACCACCATAACTGAGCTTGAACAAGAAGAACTGCTTGAGAAATTGGAAGTCTTCAAAATCAAAGGCAAAGATAAGCATGGCCGCAAGATTCTCCGCATTATCGGAAAATTCTTCCCTGCGCGATTGGTTAGCGTTGAGGTTCTGAAGAAGTTTTTGGAGGAGAGGATTTTCCCGAAGCTAGGGAAGAAGAAATTCGCGGTGTTGTATATTCACACCGGCGTTCAGAGGAGCGAGAATTTTGCCGGAATTTCTTCTCTCCGATCGGTTTACGATGCGATTCCGGCGAACGTGAAGGAGAATCTAGAAGCTGTTTATTTTATTCATCCTGGTTTACAAGCTCGCCTTTTTCTCGCTACGTTTGGCCGTTTTCTCTTCAACGCTGGGTTGTACAGGAAGCTGAGGTACATCAGCAGAGTTGGTTATCTATGGGAGAATGTTAGGAGGAACGAAGTGGAGATTCCGGAGTTCGTATACGATCATGATGAAGATTTGGAGTATCGTCCAATGATGGATTATGGATTAGAGAGTGATCATGCTAGGGTTTATGGTACTGCTCCTGCACTAGATTCGCCTGTGACTACTTACTCCATGAGGTGTATCTCATAG
Protein sequence

>Msa0712930

MSTTITELEQEELLEKLEVFKIKGKDKHGRKILRIIGKFFPARLVSVEVLKKFLEERIFPKLGKKKFAVLYIHTGVQRSENFAGISSLRSVYDAIPANVKENLEAVYFIHPGLQARLFLATFGRFLFNAGLYRKLRYISRVGYLWENVRRNEVEIPEFVYDHDEDLEYRPMMDYGLESDHARVYGTAPALDSPVTTYSMRCIS*