AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0800530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800530 MtrunA17_Chr5g0406301 98.522 203 3 0 1 203 1 203 4.48e-149 412
Msa0800530 MtrunA17_Chr7g0256341 45.930 172 93 0 14 185 51 222 7.23e-57 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800530 AT4G35750.1 75.369 203 49 1 1 203 1 202 1.96e-117 332
Msa0800530 AT3G10210.1 47.590 166 87 0 14 179 49 214 8.59e-54 172

Find 65 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATTGTGATGGTGAATT+TGG 0.185149 5_3:+13957512 None:intergenic
AGTGATCATGCTAGGGTTTA+TGG 0.201786 5_3:-13956537 Msa0800530:CDS
ATCTCATAGGGTTTAGGATT+TGG 0.237359 5_3:-13956465 Msa0800530:exon
AGAAGAACTGCTTGAGAAAT+TGG 0.241731 5_3:-13957392 Msa0800530:CDS
TCTCATAGGGTTTAGGATTT+GGG 0.254159 5_3:-13956464 Msa0800530:exon
TCAGCAGAGTTGGTTATCTA+TGG 0.276388 5_3:-13956656 Msa0800530:CDS
GTTATCTATGGGAGAATGTT+AGG 0.282831 5_3:-13956644 Msa0800530:CDS
CATCTTGATATTAATAGTTA+TGG 0.321706 5_3:-13956176 Msa0800530:three_prime_UTR
TATCGTCCAATGATGGATTA+TGG 0.323869 5_3:-13956567 Msa0800530:CDS
GGCCAAACGTAGCGAGAAAA+AGG 0.325265 5_3:+13956985 None:intergenic
AGGTGTATCTCATAGGGTTT+AGG 0.338603 5_3:-13956471 Msa0800530:exon
CGCAAGATTCTCCGCATTAT+CGG 0.351043 5_3:-13957334 Msa0800530:CDS
CTCCATGAGGTGTATCTCAT+AGG 0.352468 5_3:-13956478 Msa0800530:exon
AATTTCTTCTTCCCTAGCTT+CGG 0.354119 5_3:+13957155 None:intergenic
ATCGGTTTACGATGCGATTC+CGG 0.372506 5_3:-13957066 Msa0800530:CDS
ACAGAGCTTTCAAATAGTTA+TGG 0.379055 5_3:-13956315 Msa0800530:three_prime_UTR
ATACGATCATGATGAAGATT+TGG 0.383633 5_3:-13956592 Msa0800530:CDS
TTCTTGTTCAAGCTCAGTTA+TGG 0.385949 5_3:+13957411 None:intergenic
ACATTATATCTCAATAACTA+TGG 0.395608 5_3:+13956288 None:intergenic
TGCGTAGTGATAGAAGAAAA+AGG 0.406840 5_3:+13957462 None:intergenic
GAGGAACGAAGTGGAGATTC+CGG 0.411726 5_3:-13956622 Msa0800530:CDS
ATTTCTTCTTCCCTAGCTTC+GGG 0.419960 5_3:+13957156 None:intergenic
CGGAATTTCTTCTCTCCGAT+CGG 0.427792 5_3:-13957084 Msa0800530:CDS
CGATCGGAGAGAAGAAATTC+CGG 0.432814 5_3:+13957085 None:intergenic
AGATTCTCCTTCACGTTCGC+CGG 0.437993 5_3:+13957047 None:intergenic
TCTAATCCATAATCCATCAT+TGG 0.455280 5_3:+13956561 None:intergenic
TTTGGAGTATCGTCCAATGA+TGG 0.485084 5_3:-13956574 Msa0800530:CDS
GTTCACTGTAGTCGTTGGAT+TGG 0.491143 5_3:-13956442 Msa0800530:three_prime_UTR
TTTGGGTTCACTGTAGTCGT+TGG 0.495682 5_3:-13956447 Msa0800530:three_prime_UTR
TTGGGTGTGATAGGTTGTAC+AGG 0.501143 5_3:-13956692 Msa0800530:intron
GATGGTGAATTTGGATGGAT+AGG 0.507550 5_3:+13957521 None:intergenic
GATTAGAGAGTGATCATGCT+AGG 0.515483 5_3:-13956545 Msa0800530:CDS
TCCATGAGGTGTATCTCATA+GGG 0.515766 5_3:-13956477 Msa0800530:exon
CTGAGGTACATCAGCAGAGT+TGG 0.517318 5_3:-13956666 Msa0800530:CDS
ACCCAGCGTTGAAGAGAAAA+CGG 0.517837 5_3:+13956964 None:intergenic
CAAGACCTAAAGAAAACAAA+AGG 0.523861 5_3:+13956235 None:intergenic
TTGGAAGTCTTCAAAATCAA+AGG 0.533957 5_3:-13957373 Msa0800530:CDS
AGCGCGATTGGTTAGCGTTG+AGG 0.536404 5_3:-13957210 Msa0800530:CDS
ATGGTGAATTTGGATGGATA+GGG 0.536686 5_3:+13957522 None:intergenic
TTGTTCAAGCTCAGTTATGG+TGG 0.540444 5_3:+13957414 None:intergenic
CAGCAGAGTTGGTTATCTAT+GGG 0.551346 5_3:-13956655 Msa0800530:CDS
GAGCTTTCAAATAGTTATGG+AGG 0.553818 5_3:-13956312 Msa0800530:three_prime_UTR
GTCACAGGCGAATCTAGTGC+AGG 0.559387 5_3:+13956507 None:intergenic
AAAAGGCGAGCTTGTAAACC+AGG 0.560268 5_3:+13957002 None:intergenic
ATTGTGATGGTGAATTTGGA+TGG 0.571335 5_3:+13957516 None:intergenic
ATTATCGGAAAATTCTTCCC+TGG 0.573008 5_3:-13957319 Msa0800530:intron
TTGATATTAATAGTTATGGA+AGG 0.590651 5_3:-13956172 Msa0800530:three_prime_UTR
TGCGATTCCGGCGAACGTGA+AGG 0.593355 5_3:-13957054 Msa0800530:CDS
AGCTTTCAAATAGTTATGGA+GGG 0.593526 5_3:-13956311 Msa0800530:three_prime_UTR
ATTAGAGAGTGATCATGCTA+GGG 0.594329 5_3:-13956544 Msa0800530:CDS
CTCATGGAGTAAGTAGTCAC+AGG 0.597762 5_3:+13956492 None:intergenic
ACCCTATGAGATACACCTCA+TGG 0.597933 5_3:+13956476 None:intergenic
TCATGATCGTATACGAACTC+CGG 0.600493 5_3:+13956603 None:intergenic
GAATGTTAGGAGGAACGAAG+TGG 0.610161 5_3:-13956631 Msa0800530:CDS
CGATAATGCGGAGAATCTTG+CGG 0.614669 5_3:+13957335 None:intergenic
CGTGATGATTATGATTGTGA+TGG 0.623135 5_3:+13957503 None:intergenic
ATATTCACACCGGCGTTCAG+AGG 0.623653 5_3:-13957121 Msa0800530:CDS
ATCAAAGGCAAAGATAAGCA+TGG 0.637350 5_3:-13957358 Msa0800530:CDS
GAATCGCATCGTAAACCGAT+CGG 0.641309 5_3:+13957069 None:intergenic
ATCTATGGGAGAATGTTAGG+AGG 0.655849 5_3:-13956641 Msa0800530:CDS
ATAGGTTGTACAGGAAGCTG+AGG 0.667654 5_3:-13956683 Msa0800530:intron
ATTCTCGCTCCTCTGAACGC+CGG 0.670180 5_3:+13957112 None:intergenic
GCTAGGGAAGAAGAAATTCG+CGG 0.672425 5_3:-13957150 Msa0800530:CDS
CTGTGACTACTTACTCCATG+AGG 0.695115 5_3:-13956491 Msa0800530:CDS
GCGGTGTTGTATATTCACAC+CGG 0.721011 5_3:-13957131 Msa0800530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAATCACATATATAA+CGG + chr5_3:13956380-13956399 None:intergenic 15.0%
!!! GATATTTTCATTTTTTTTGT+TGG - chr5_3:13957498-13957517 Msa0800530:five_prime_UTR 15.0%
!! AACAAAAATCACATATATAA+CGG + chr5_3:13956380-13956399 None:intergenic 15.0%
!!! GATATTTTCATTTTTTTTGT+TGG - chr5_3:13957498-13957517 Msa0800530:five_prime_UTR 15.0%
!!! AACTGAAATAATTTTTACCA+GGG + chr5_3:13956349-13956368 None:intergenic 20.0%
!!! AAACTGAAATAATTTTTACC+AGG + chr5_3:13956350-13956369 None:intergenic 20.0%
!!! GATTAATTTAAAGCTGATTT+AGG + chr5_3:13956767-13956786 None:intergenic 20.0%
!! AAATTAATGAAGTTGTTTGT+AGG - chr5_3:13956887-13956906 Msa0800530:intron 20.0%
!!! TATTAATCGGAAGTTTTAAT+TGG - chr5_3:13956916-13956935 Msa0800530:intron 20.0%
!! ACATTATATCTCAATAACTA+TGG + chr5_3:13957363-13957382 None:intergenic 20.0%
!! CATCTTGATATTAATAGTTA+TGG - chr5_3:13957472-13957491 Msa0800530:five_prime_UTR 20.0%
!! TTGATATTAATAGTTATGGA+AGG - chr5_3:13957476-13957495 Msa0800530:five_prime_UTR 20.0%
!!! TTTTTTGTTGGATAATTCAA+TGG - chr5_3:13957510-13957529 Msa0800530:five_prime_UTR 20.0%
!!! AACTGAAATAATTTTTACCA+GGG + chr5_3:13956349-13956368 None:intergenic 20.0%
!!! AAACTGAAATAATTTTTACC+AGG + chr5_3:13956350-13956369 None:intergenic 20.0%
!!! GATTAATTTAAAGCTGATTT+AGG + chr5_3:13956767-13956786 None:intergenic 20.0%
!! AAATTAATGAAGTTGTTTGT+AGG - chr5_3:13956887-13956906 Msa0800530:intron 20.0%
!!! TATTAATCGGAAGTTTTAAT+TGG - chr5_3:13956916-13956935 Msa0800530:intron 20.0%
!! ACATTATATCTCAATAACTA+TGG + chr5_3:13957363-13957382 None:intergenic 20.0%
!! CATCTTGATATTAATAGTTA+TGG - chr5_3:13957472-13957491 Msa0800530:five_prime_UTR 20.0%
!! TTGATATTAATAGTTATGGA+AGG - chr5_3:13957476-13957495 Msa0800530:five_prime_UTR 20.0%
!!! TTTTTTGTTGGATAATTCAA+TGG - chr5_3:13957510-13957529 Msa0800530:five_prime_UTR 20.0%
! TTGTAGGTTTCGTTATTAAT+CGG - chr5_3:13956903-13956922 Msa0800530:intron 25.0%
!!! AAGTTTTAATTGGCTAATGT+TGG - chr5_3:13956926-13956945 Msa0800530:intron 25.0%
!!! AGTTTTAATTGGCTAATGTT+GGG - chr5_3:13956927-13956946 Msa0800530:intron 25.0%
! TTGTAGGTTTCGTTATTAAT+CGG - chr5_3:13956903-13956922 Msa0800530:intron 25.0%
!!! AAGTTTTAATTGGCTAATGT+TGG - chr5_3:13956926-13956945 Msa0800530:intron 25.0%
!!! AGTTTTAATTGGCTAATGTT+GGG - chr5_3:13956927-13956946 Msa0800530:intron 25.0%
! TATGATTGTGATGGTGAATT+TGG + chr5_3:13956139-13956158 None:intergenic 30.0%
TTGGAAGTCTTCAAAATCAA+AGG - chr5_3:13956275-13956294 Msa0800530:three_prime_UTR 30.0%
!!! GCTGTTTATTTTATTCATCC+TGG - chr5_3:13956628-13956647 Msa0800530:CDS 30.0%
ATACGATCATGATGAAGATT+TGG - chr5_3:13957056-13957075 Msa0800530:CDS 30.0%
TCTAATCCATAATCCATCAT+TGG + chr5_3:13957090-13957109 None:intergenic 30.0%
ACAGAGCTTTCAAATAGTTA+TGG - chr5_3:13957333-13957352 Msa0800530:CDS 30.0%
AGCTTTCAAATAGTTATGGA+GGG - chr5_3:13957337-13957356 Msa0800530:CDS 30.0%
!!! TGTTGCCTTTTGTTTTCTTT+AGG - chr5_3:13957408-13957427 Msa0800530:CDS 30.0%
CAAGACCTAAAGAAAACAAA+AGG + chr5_3:13957416-13957435 None:intergenic 30.0%
! TATGATTGTGATGGTGAATT+TGG + chr5_3:13956139-13956158 None:intergenic 30.0%
TTGGAAGTCTTCAAAATCAA+AGG - chr5_3:13956275-13956294 Msa0800530:three_prime_UTR 30.0%
!!! GCTGTTTATTTTATTCATCC+TGG - chr5_3:13956628-13956647 Msa0800530:CDS 30.0%
ATACGATCATGATGAAGATT+TGG - chr5_3:13957056-13957075 Msa0800530:CDS 30.0%
TCTAATCCATAATCCATCAT+TGG + chr5_3:13957090-13957109 None:intergenic 30.0%
ACAGAGCTTTCAAATAGTTA+TGG - chr5_3:13957333-13957352 Msa0800530:CDS 30.0%
AGCTTTCAAATAGTTATGGA+GGG - chr5_3:13957337-13957356 Msa0800530:CDS 30.0%
!!! TGTTGCCTTTTGTTTTCTTT+AGG - chr5_3:13957408-13957427 Msa0800530:CDS 30.0%
CAAGACCTAAAGAAAACAAA+AGG + chr5_3:13957416-13957435 None:intergenic 30.0%
ATGGTGAATTTGGATGGATA+GGG + chr5_3:13956129-13956148 None:intergenic 35.0%
! ATTGTGATGGTGAATTTGGA+TGG + chr5_3:13956135-13956154 None:intergenic 35.0%
CGTGATGATTATGATTGTGA+TGG + chr5_3:13956148-13956167 None:intergenic 35.0%
TGCGTAGTGATAGAAGAAAA+AGG + chr5_3:13956189-13956208 None:intergenic 35.0%
TTCTTGTTCAAGCTCAGTTA+TGG + chr5_3:13956240-13956259 None:intergenic 35.0%
AGAAGAACTGCTTGAGAAAT+TGG - chr5_3:13956256-13956275 Msa0800530:three_prime_UTR 35.0%
ATCAAAGGCAAAGATAAGCA+TGG - chr5_3:13956290-13956309 Msa0800530:three_prime_UTR 35.0%
! GGAAGAATTTTCCGATAATG+CGG + chr5_3:13956328-13956347 None:intergenic 35.0%
ATTATCGGAAAATTCTTCCC+TGG - chr5_3:13956329-13956348 Msa0800530:three_prime_UTR 35.0%
!!! ATTTTTTGATTCAGCGCGAT+TGG - chr5_3:13956426-13956445 Msa0800530:three_prime_UTR 35.0%
!!! TGAGGTTCTGAAGAAGTTTT+TGG - chr5_3:13956456-13956475 Msa0800530:three_prime_UTR 35.0%
AATTTCTTCTTCCCTAGCTT+CGG + chr5_3:13956496-13956515 None:intergenic 35.0%
!!! GCTAATGTTGGGTTTTGATT+GGG - chr5_3:13956938-13956957 Msa0800530:intron 35.0%
GTTATCTATGGGAGAATGTT+AGG - chr5_3:13957004-13957023 Msa0800530:CDS 35.0%
!! TATCGTCCAATGATGGATTA+TGG - chr5_3:13957081-13957100 Msa0800530:CDS 35.0%
ATTAGAGAGTGATCATGCTA+GGG - chr5_3:13957104-13957123 Msa0800530:CDS 35.0%
! ATCTCATAGGGTTTAGGATT+TGG - chr5_3:13957183-13957202 Msa0800530:CDS 35.0%
! TCTCATAGGGTTTAGGATTT+GGG - chr5_3:13957184-13957203 Msa0800530:CDS 35.0%
GAGCTTTCAAATAGTTATGG+AGG - chr5_3:13957336-13957355 Msa0800530:CDS 35.0%
ATGGTGAATTTGGATGGATA+GGG + chr5_3:13956129-13956148 None:intergenic 35.0%
! ATTGTGATGGTGAATTTGGA+TGG + chr5_3:13956135-13956154 None:intergenic 35.0%
CGTGATGATTATGATTGTGA+TGG + chr5_3:13956148-13956167 None:intergenic 35.0%
TGCGTAGTGATAGAAGAAAA+AGG + chr5_3:13956189-13956208 None:intergenic 35.0%
TTCTTGTTCAAGCTCAGTTA+TGG + chr5_3:13956240-13956259 None:intergenic 35.0%
AGAAGAACTGCTTGAGAAAT+TGG - chr5_3:13956256-13956275 Msa0800530:three_prime_UTR 35.0%
ATCAAAGGCAAAGATAAGCA+TGG - chr5_3:13956290-13956309 Msa0800530:three_prime_UTR 35.0%
! GGAAGAATTTTCCGATAATG+CGG + chr5_3:13956328-13956347 None:intergenic 35.0%
ATTATCGGAAAATTCTTCCC+TGG - chr5_3:13956329-13956348 Msa0800530:three_prime_UTR 35.0%
!!! ATTTTTTGATTCAGCGCGAT+TGG - chr5_3:13956426-13956445 Msa0800530:three_prime_UTR 35.0%
!!! TGAGGTTCTGAAGAAGTTTT+TGG - chr5_3:13956456-13956475 Msa0800530:three_prime_UTR 35.0%
AATTTCTTCTTCCCTAGCTT+CGG + chr5_3:13956496-13956515 None:intergenic 35.0%
!!! GCTAATGTTGGGTTTTGATT+GGG - chr5_3:13956938-13956957 Msa0800530:intron 35.0%
GTTATCTATGGGAGAATGTT+AGG - chr5_3:13957004-13957023 Msa0800530:CDS 35.0%
!! TATCGTCCAATGATGGATTA+TGG - chr5_3:13957081-13957100 Msa0800530:CDS 35.0%
ATTAGAGAGTGATCATGCTA+GGG - chr5_3:13957104-13957123 Msa0800530:CDS 35.0%
! ATCTCATAGGGTTTAGGATT+TGG - chr5_3:13957183-13957202 Msa0800530:CDS 35.0%
! TCTCATAGGGTTTAGGATTT+GGG - chr5_3:13957184-13957203 Msa0800530:CDS 35.0%
GAGCTTTCAAATAGTTATGG+AGG - chr5_3:13957336-13957355 Msa0800530:CDS 35.0%
GATGGTGAATTTGGATGGAT+AGG + chr5_3:13956130-13956149 None:intergenic 40.0%
TTGTTCAAGCTCAGTTATGG+TGG + chr5_3:13956237-13956256 None:intergenic 40.0%
!!! GGTTCTGAAGAAGTTTTTGG+AGG - chr5_3:13956459-13956478 Msa0800530:three_prime_UTR 40.0%
!! TGAAGAAGTTTTTGGAGGAG+AGG - chr5_3:13956464-13956483 Msa0800530:exon 40.0%
ATTTCTTCTTCCCTAGCTTC+GGG + chr5_3:13956495-13956514 None:intergenic 40.0%
!! GGCTAATGTTGGGTTTTGAT+TGG - chr5_3:13956937-13956956 Msa0800530:intron 40.0%
! TCAGCAGAGTTGGTTATCTA+TGG - chr5_3:13956992-13957011 Msa0800530:CDS 40.0%
! CAGCAGAGTTGGTTATCTAT+GGG - chr5_3:13956993-13957012 Msa0800530:CDS 40.0%
ATCTATGGGAGAATGTTAGG+AGG - chr5_3:13957007-13957026 Msa0800530:CDS 40.0%
TCATGATCGTATACGAACTC+CGG + chr5_3:13957048-13957067 None:intergenic 40.0%
!! TTTGGAGTATCGTCCAATGA+TGG - chr5_3:13957074-13957093 Msa0800530:CDS 40.0%
GATTAGAGAGTGATCATGCT+AGG - chr5_3:13957103-13957122 Msa0800530:CDS 40.0%
AGTGATCATGCTAGGGTTTA+TGG - chr5_3:13957111-13957130 Msa0800530:CDS 40.0%
TCCATGAGGTGTATCTCATA+GGG - chr5_3:13957171-13957190 Msa0800530:CDS 40.0%
AGGTGTATCTCATAGGGTTT+AGG - chr5_3:13957177-13957196 Msa0800530:CDS 40.0%
GATGGTGAATTTGGATGGAT+AGG + chr5_3:13956130-13956149 None:intergenic 40.0%
TTGTTCAAGCTCAGTTATGG+TGG + chr5_3:13956237-13956256 None:intergenic 40.0%
!!! GGTTCTGAAGAAGTTTTTGG+AGG - chr5_3:13956459-13956478 Msa0800530:three_prime_UTR 40.0%
!! TGAAGAAGTTTTTGGAGGAG+AGG - chr5_3:13956464-13956483 Msa0800530:exon 40.0%
ATTTCTTCTTCCCTAGCTTC+GGG + chr5_3:13956495-13956514 None:intergenic 40.0%
!! GGCTAATGTTGGGTTTTGAT+TGG - chr5_3:13956937-13956956 Msa0800530:intron 40.0%
! TCAGCAGAGTTGGTTATCTA+TGG - chr5_3:13956992-13957011 Msa0800530:CDS 40.0%
! CAGCAGAGTTGGTTATCTAT+GGG - chr5_3:13956993-13957012 Msa0800530:CDS 40.0%
ATCTATGGGAGAATGTTAGG+AGG - chr5_3:13957007-13957026 Msa0800530:CDS 40.0%
TCATGATCGTATACGAACTC+CGG + chr5_3:13957048-13957067 None:intergenic 40.0%
!! TTTGGAGTATCGTCCAATGA+TGG - chr5_3:13957074-13957093 Msa0800530:CDS 40.0%
GATTAGAGAGTGATCATGCT+AGG - chr5_3:13957103-13957122 Msa0800530:CDS 40.0%
AGTGATCATGCTAGGGTTTA+TGG - chr5_3:13957111-13957130 Msa0800530:CDS 40.0%
TCCATGAGGTGTATCTCATA+GGG - chr5_3:13957171-13957190 Msa0800530:CDS 40.0%
AGGTGTATCTCATAGGGTTT+AGG - chr5_3:13957177-13957196 Msa0800530:CDS 40.0%
CGATAATGCGGAGAATCTTG+CGG + chr5_3:13956316-13956335 None:intergenic 45.0%
CGCAAGATTCTCCGCATTAT+CGG - chr5_3:13956314-13956333 Msa0800530:three_prime_UTR 45.0%
! AGAGGATTTTCCCGAAGCTA+GGG - chr5_3:13956482-13956501 Msa0800530:CDS 45.0%
GCTAGGGAAGAAGAAATTCG+CGG - chr5_3:13956498-13956517 Msa0800530:CDS 45.0%
!! GCGGTGTTGTATATTCACAC+CGG - chr5_3:13956517-13956536 Msa0800530:CDS 45.0%
CGATCGGAGAGAAGAAATTC+CGG + chr5_3:13956566-13956585 None:intergenic 45.0%
CGGAATTTCTTCTCTCCGAT+CGG - chr5_3:13956564-13956583 Msa0800530:CDS 45.0%
GAATCGCATCGTAAACCGAT+CGG + chr5_3:13956582-13956601 None:intergenic 45.0%
! ATCGGTTTACGATGCGATTC+CGG - chr5_3:13956582-13956601 Msa0800530:CDS 45.0%
AAAAGGCGAGCTTGTAAACC+AGG + chr5_3:13956649-13956668 None:intergenic 45.0%
! ACCCAGCGTTGAAGAGAAAA+CGG + chr5_3:13956687-13956706 None:intergenic 45.0%
!!! GGGTTTTGATTGGGTGTGAT+AGG - chr5_3:13956947-13956966 Msa0800530:intron 45.0%
TTGGGTGTGATAGGTTGTAC+AGG - chr5_3:13956956-13956975 Msa0800530:intron 45.0%
ATAGGTTGTACAGGAAGCTG+AGG - chr5_3:13956965-13956984 Msa0800530:intron 45.0%
GAATGTTAGGAGGAACGAAG+TGG - chr5_3:13957017-13957036 Msa0800530:CDS 45.0%
CTCATGGAGTAAGTAGTCAC+AGG + chr5_3:13957159-13957178 None:intergenic 45.0%
CTGTGACTACTTACTCCATG+AGG - chr5_3:13957157-13957176 Msa0800530:CDS 45.0%
CTCCATGAGGTGTATCTCAT+AGG - chr5_3:13957170-13957189 Msa0800530:CDS 45.0%
ACCCTATGAGATACACCTCA+TGG + chr5_3:13957175-13957194 None:intergenic 45.0%
! TTTGGGTTCACTGTAGTCGT+TGG - chr5_3:13957201-13957220 Msa0800530:CDS 45.0%
GTTCACTGTAGTCGTTGGAT+TGG - chr5_3:13957206-13957225 Msa0800530:CDS 45.0%
CGATAATGCGGAGAATCTTG+CGG + chr5_3:13956316-13956335 None:intergenic 45.0%
CGCAAGATTCTCCGCATTAT+CGG - chr5_3:13956314-13956333 Msa0800530:three_prime_UTR 45.0%
! AGAGGATTTTCCCGAAGCTA+GGG - chr5_3:13956482-13956501 Msa0800530:CDS 45.0%
GCTAGGGAAGAAGAAATTCG+CGG - chr5_3:13956498-13956517 Msa0800530:CDS 45.0%
!! GCGGTGTTGTATATTCACAC+CGG - chr5_3:13956517-13956536 Msa0800530:CDS 45.0%
CGATCGGAGAGAAGAAATTC+CGG + chr5_3:13956566-13956585 None:intergenic 45.0%
CGGAATTTCTTCTCTCCGAT+CGG - chr5_3:13956564-13956583 Msa0800530:CDS 45.0%
GAATCGCATCGTAAACCGAT+CGG + chr5_3:13956582-13956601 None:intergenic 45.0%
! ATCGGTTTACGATGCGATTC+CGG - chr5_3:13956582-13956601 Msa0800530:CDS 45.0%
AAAAGGCGAGCTTGTAAACC+AGG + chr5_3:13956649-13956668 None:intergenic 45.0%
! ACCCAGCGTTGAAGAGAAAA+CGG + chr5_3:13956687-13956706 None:intergenic 45.0%
!!! GGGTTTTGATTGGGTGTGAT+AGG - chr5_3:13956947-13956966 Msa0800530:intron 45.0%
TTGGGTGTGATAGGTTGTAC+AGG - chr5_3:13956956-13956975 Msa0800530:intron 45.0%
ATAGGTTGTACAGGAAGCTG+AGG - chr5_3:13956965-13956984 Msa0800530:intron 45.0%
GAATGTTAGGAGGAACGAAG+TGG - chr5_3:13957017-13957036 Msa0800530:CDS 45.0%
CTCATGGAGTAAGTAGTCAC+AGG + chr5_3:13957159-13957178 None:intergenic 45.0%
CTGTGACTACTTACTCCATG+AGG - chr5_3:13957157-13957176 Msa0800530:CDS 45.0%
CTCCATGAGGTGTATCTCAT+AGG - chr5_3:13957170-13957189 Msa0800530:CDS 45.0%
ACCCTATGAGATACACCTCA+TGG + chr5_3:13957175-13957194 None:intergenic 45.0%
! TTTGGGTTCACTGTAGTCGT+TGG - chr5_3:13957201-13957220 Msa0800530:CDS 45.0%
GTTCACTGTAGTCGTTGGAT+TGG - chr5_3:13957206-13957225 Msa0800530:CDS 45.0%
! GAGAGGATTTTCCCGAAGCT+AGG - chr5_3:13956481-13956500 Msa0800530:CDS 50.0%
ATATTCACACCGGCGTTCAG+AGG - chr5_3:13956527-13956546 Msa0800530:CDS 50.0%
! CAGAGGAGCGAGAATTTTGC+CGG - chr5_3:13956544-13956563 Msa0800530:CDS 50.0%
AGATTCTCCTTCACGTTCGC+CGG + chr5_3:13956604-13956623 None:intergenic 50.0%
! CGCCTTTTTCTCGCTACGTT+TGG - chr5_3:13956661-13956680 Msa0800530:CDS 50.0%
GGCCAAACGTAGCGAGAAAA+AGG + chr5_3:13956666-13956685 None:intergenic 50.0%
!! GCCGTTTTCTCTTCAACGCT+GGG - chr5_3:13956683-13956702 Msa0800530:intron 50.0%
CTGAGGTACATCAGCAGAGT+TGG - chr5_3:13956982-13957001 Msa0800530:CDS 50.0%
GAGGAACGAAGTGGAGATTC+CGG - chr5_3:13957026-13957045 Msa0800530:CDS 50.0%
! GAGAGGATTTTCCCGAAGCT+AGG - chr5_3:13956481-13956500 Msa0800530:CDS 50.0%
ATATTCACACCGGCGTTCAG+AGG - chr5_3:13956527-13956546 Msa0800530:CDS 50.0%
! CAGAGGAGCGAGAATTTTGC+CGG - chr5_3:13956544-13956563 Msa0800530:CDS 50.0%
AGATTCTCCTTCACGTTCGC+CGG + chr5_3:13956604-13956623 None:intergenic 50.0%
! CGCCTTTTTCTCGCTACGTT+TGG - chr5_3:13956661-13956680 Msa0800530:CDS 50.0%
GGCCAAACGTAGCGAGAAAA+AGG + chr5_3:13956666-13956685 None:intergenic 50.0%
!! GCCGTTTTCTCTTCAACGCT+GGG - chr5_3:13956683-13956702 Msa0800530:intron 50.0%
CTGAGGTACATCAGCAGAGT+TGG - chr5_3:13956982-13957001 Msa0800530:CDS 50.0%
GAGGAACGAAGTGGAGATTC+CGG - chr5_3:13957026-13957045 Msa0800530:CDS 50.0%
! AGCGCGATTGGTTAGCGTTG+AGG - chr5_3:13956438-13956457 Msa0800530:three_prime_UTR 55.0%
ATTCTCGCTCCTCTGAACGC+CGG + chr5_3:13956539-13956558 None:intergenic 55.0%
!! GGCCGTTTTCTCTTCAACGC+TGG - chr5_3:13956682-13956701 Msa0800530:CDS 55.0%
GTCACAGGCGAATCTAGTGC+AGG + chr5_3:13957144-13957163 None:intergenic 55.0%
! AGCGCGATTGGTTAGCGTTG+AGG - chr5_3:13956438-13956457 Msa0800530:three_prime_UTR 55.0%
ATTCTCGCTCCTCTGAACGC+CGG + chr5_3:13956539-13956558 None:intergenic 55.0%
!! GGCCGTTTTCTCTTCAACGC+TGG - chr5_3:13956682-13956701 Msa0800530:CDS 55.0%
GTCACAGGCGAATCTAGTGC+AGG + chr5_3:13957144-13957163 None:intergenic 55.0%
TGCGATTCCGGCGAACGTGA+AGG - chr5_3:13956594-13956613 Msa0800530:CDS 60.0%
TGCGATTCCGGCGAACGTGA+AGG - chr5_3:13956594-13956613 Msa0800530:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 13956119 13957551 13956119 ID=Msa0800530;Name=Msa0800530
chr5_3 mRNA 13956119 13957551 13956119 ID=Msa0800530-mRNA-1;Parent=Msa0800530;Name=Msa0800530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=108|1|1|1|1|1|3|360|203
chr5_3 exon 13956119 13956701 13956119 ID=Msa0800530-mRNA-1:exon:5839;Parent=Msa0800530-mRNA-1
chr5_3 exon 13956966 13957230 13956966 ID=Msa0800530-mRNA-1:exon:5838;Parent=Msa0800530-mRNA-1
chr5_3 exon 13957320 13957551 13957320 ID=Msa0800530-mRNA-1:exon:5837;Parent=Msa0800530-mRNA-1
chr5_3 five_prime_UTR 13957444 13957551 13957444 ID=Msa0800530-mRNA-1:five_prime_utr;Parent=Msa0800530-mRNA-1
chr5_3 CDS 13957320 13957443 13957320 ID=Msa0800530-mRNA-1:cds;Parent=Msa0800530-mRNA-1
chr5_3 CDS 13956966 13957230 13956966 ID=Msa0800530-mRNA-1:cds;Parent=Msa0800530-mRNA-1
chr5_3 CDS 13956479 13956701 13956479 ID=Msa0800530-mRNA-1:cds;Parent=Msa0800530-mRNA-1
chr5_3 three_prime_UTR 13956119 13956478 13956119 ID=Msa0800530-mRNA-1:three_prime_utr;Parent=Msa0800530-mRNA-1
Gene Sequence

>Msa0800530

ATGAGCACCACCATAACTGAGCTTGAACAAGAAGAACTGCTTGAGAAATTGGAAGTCTTCAAAATCAAAGGCAAAGATAAGCATGGCCGCAAGATTCTCCGCATTATCGGAAAATTCTTCCCTGCGCGATTGGTTAGCGTTGAGGTTCTGAAGAAGTTTTTGGAGGAGAGGATTTTCCCGAAGCTAGGGAAGAAGAAATTCGCGGTGTTGTATATTCACACCGGCGTTCAGAGGAGCGAGAATTTTGCCGGAATTTCTTCTCTCCGATCGGTTTACGATGCGATTCCGGCGAACGTGAAGGAGAATCTAGAAGCTGTTTATTTTATTCATCCTGGTTTACAAGCTCGCCTTTTTCTCGCTACGTTTGGCCGTTTTCTCTTCAACGCTGGGTTGTACAGGAAGCTGAGGTACATCAGCAGAGTTGGTTATCTATGGGAGAATGTTAGGAGGAACGAAGTGGAGATTCCGGAGTTCGTATACGATCATGATGAAGATTTGGAGTATCGTCCAATGATGGATTATGGATTAGAGAGTGATCATGCTAGGGTTTATGGTACTGCTCCTGCACTAGATTCGCCTGTGACTACTTACTCCATGAGGTGTATCTCATAG
Protein sequence

>Msa0800530

MSTTITELEQEELLEKLEVFKIKGKDKHGRKILRIIGKFFPARLVSVEVLKKFLEERIFPKLGKKKFAVLYIHTGVQRSENFAGISSLRSVYDAIPANVKENLEAVYFIHPGLQARLFLATFGRFLFNAGLYRKLRYISRVGYLWENVRRNEVEIPEFVYDHDEDLEYRPMMDYGLESDHARVYGTAPALDSPVTTYSMRCIS*