AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0843490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0843490 MtrunA17_Chr3g0087301 54.255 188 46 1 1 148 894 1081 1.31e-53 182
Msa0843490 MtrunA17_Chr5g0414211 46.970 198 42 2 1 158 229 403 4.99e-45 152
Msa0843490 MtrunA17_Chr5g0414311 41.451 193 72 2 1 152 914 1106 1.98e-42 150
Msa0843490 MtrunA17_Chr5g0415471 42.857 189 67 2 1 148 911 1099 3.86e-42 149
Msa0843490 MtrunA17_Chr5g0415481 54.472 123 46 4 2 115 338 459 3.16e-33 122
Msa0843490 MtrunA17_Chr5g0415511 48.344 151 38 1 38 148 549 699 3.32e-33 124
Msa0843490 MtrunA17_Chr4g0027251 37.162 148 83 3 1 147 905 1043 4.07e-23 95.1
Msa0843490 MtrunA17_Chr5g0414181 66.176 68 23 0 1 68 905 972 1.87e-20 87.4
Msa0843490 MtrunA17_Chr6g0468101 28.708 209 84 4 4 150 852 1057 4.67e-20 86.3
Msa0843490 MtrunA17_Chr3g0085931 29.907 214 87 4 4 154 893 1106 7.19e-20 85.9
Msa0843490 MtrunA17_Chr6g0468021 29.703 202 78 4 4 141 868 1069 1.83e-18 81.6
Msa0843490 MtrunA17_Chr5g0430291 34.247 146 64 4 1 146 902 1015 2.39e-18 81.3
Msa0843490 MtrunA17_Chr6g0468381 28.713 202 82 3 7 146 308 509 1.03e-17 79.3
Msa0843490 MtrunA17_Chr8g0351811 29.187 209 86 5 4 150 415 623 1.04e-17 79.3
Msa0843490 MtrunA17_Chr6g0468331 28.061 196 79 4 8 141 476 671 1.29e-16 76.3
Msa0843490 MtrunA17_Chr5g0414301 38.776 147 76 4 1 144 432 567 1.39e-16 76.3
Msa0843490 MtrunA17_Chr7g0244231 34.247 146 64 4 1 146 893 1006 2.46e-16 75.5
Msa0843490 MtrunA17_Chr6g0468341 28.095 210 85 3 7 150 698 907 2.87e-16 75.5
Msa0843490 MtrunA17_Chr1g0155321 32.877 146 66 4 1 146 895 1008 5.25e-16 74.7
Msa0843490 MtrunA17_Chr3g0123111 28.571 196 79 4 7 141 890 1085 1.24e-15 73.6
Msa0843490 MtrunA17_Chr5g0430721 32.192 146 67 4 1 146 940 1053 1.67e-15 73.2
Msa0843490 MtrunA17_Chr5g0430641 35.833 120 68 2 26 144 898 1009 2.54e-15 72.8
Msa0843490 MtrunA17_Chr6g0467921 29.412 204 83 6 4 146 103 306 4.08e-15 71.6
Msa0843490 MtrunA17_Chr6g0468121 27.830 212 88 6 4 150 871 1082 1.16e-14 70.9
Msa0843490 MtrunA17_Chr6g0468201 27.778 198 81 5 6 141 477 674 2.61e-14 69.7
Msa0843490 MtrunA17_Chr5g0410481 32.192 146 87 5 2 144 935 1071 3.24e-14 69.3
Msa0843490 MtrunA17_Chr5g0430621 31.507 146 68 3 1 146 898 1011 3.41e-14 69.3
Msa0843490 MtrunA17_Chr5g0430511 35.246 122 65 3 26 147 903 1010 3.72e-14 69.3
Msa0843490 MtrunA17_Chr8g0352861 31.937 191 81 8 7 152 1058 1244 4.18e-14 68.9
Msa0843490 MtrunA17_Chr6g0470011 28.141 199 82 5 4 141 888 1086 9.19e-14 68.2
Msa0843490 MtrunA17_Chr6g0468151 29.592 196 74 5 16 147 896 1091 1.02e-13 68.2
Msa0843490 MtrunA17_Chr6g0468131 27.404 208 87 4 4 147 145 352 1.05e-13 67.4
Msa0843490 MtrunA17_Chr6g0468451 24.299 214 96 3 5 152 893 1106 2.25e-13 67.0
Msa0843490 MtrunA17_Chr8g0355411 29.268 164 97 4 3 148 681 843 2.55e-13 66.6
Msa0843490 MtrunA17_Chr5g0430581 33.775 151 63 6 1 146 862 980 4.81e-13 65.9
Msa0843490 MtrunA17_Chr6g0470081 28.643 199 81 6 4 141 888 1086 5.27e-13 65.9
Msa0843490 MtrunA17_Chr5g0430441 32.653 147 64 5 2 147 876 988 1.69e-12 64.3
Msa0843490 MtrunA17_Chr6g0470001 27.536 207 89 5 4 149 495 701 3.82e-12 63.5
Msa0843490 MtrunA17_Chr5g0431051 33.108 148 64 5 1 144 871 987 4.33e-12 63.2
Msa0843490 MtrunA17_Chr5g0410161 29.697 165 97 4 2 147 1072 1236 6.31e-12 62.8
Msa0843490 MtrunA17_Chr6g0468251 25.604 207 90 3 5 147 233 439 8.43e-12 62.4
Msa0843490 MtrunA17_Chr3g0082131 28.395 162 87 4 8 150 1059 1210 1.38e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTATTTCTGAAGAATTT+TGG 0.096269 5_4:+21143429 None:intergenic
ATTTATGGTTGCAGTGAGTT+AGG 0.250431 5_4:-21143215 Msa0843490:intron
AACAGTAAGGTTACTGATTT+CGG 0.271928 5_4:+21143613 None:intergenic
CATTGATGTACAAAACAAAA+TGG 0.332678 5_4:-21143135 Msa0843490:CDS
CGCGGATTTAATTTGATATG+AGG 0.337188 5_4:-21142395 Msa0843490:three_prime_UTR
CAAAATTCTTCAGAAATAGA+AGG 0.344587 5_4:-21143428 Msa0843490:CDS
AGATTGAATGTATCTTAGTT+AGG 0.344954 5_4:-21142365 Msa0843490:three_prime_UTR
CTTAAGATATTGAATATTGC+GGG 0.346575 5_4:-21143515 Msa0843490:CDS
AATTTGTGATCATTATTTCT+TGG 0.363068 5_4:+21143588 None:intergenic
GAAGTGGTTCTCCTGAAGTT+GGG 0.403337 5_4:-21142466 Msa0843490:three_prime_UTR
TTTATGGTTGCAGTGAGTTA+GGG 0.415060 5_4:-21143214 Msa0843490:intron
CTTCGTGTAGGCCCAACTTC+AGG 0.418748 5_4:+21142455 None:intergenic
TCAGGTAGGGATGCCAGGTT+TGG 0.436582 5_4:+21143347 None:intergenic
ACAAAACTTCGATTTAATCA+AGG 0.444149 5_4:-21142277 Msa0843490:three_prime_UTR
ACCAGTCAGGTAGGGATGCC+AGG 0.448571 5_4:+21143342 None:intergenic
TGAAGTGGTTCTCCTGAAGT+TGG 0.451263 5_4:-21142467 Msa0843490:three_prime_UTR
ACGAAACCTTACTTCCCTAA+AGG 0.460750 5_4:-21143678 Msa0843490:CDS
CTTCTCAATGCTGTTTGAAG+TGG 0.462373 5_4:-21142482 Msa0843490:exon
GGCTGGGAGTTGCTCAAGGT+TGG 0.466786 5_4:+21143634 None:intergenic
TAGCAAGGATAAACTGTTGA+AGG 0.476209 5_4:+21142423 None:intergenic
TTATTTCTTGGATAACAGTA+AGG 0.481390 5_4:+21143600 None:intergenic
GTAAGGTTACTGATTTCGGC+TGG 0.482083 5_4:+21143617 None:intergenic
ACCTGGCATCCCTACCTGAC+TGG 0.504515 5_4:-21143343 Msa0843490:intron
TTTCGGCTGGGAGTTGCTCA+AGG 0.507552 5_4:+21143630 None:intergenic
CCTAGGTTTCCTAACCAGTC+AGG 0.511917 5_4:+21143329 None:intergenic
ATGGCCTACGTAGGAGGAAG+AGG 0.520815 5_4:-21143110 Msa0843490:intron
GCGGATTTAATTTGATATGA+GGG 0.524095 5_4:-21142394 Msa0843490:three_prime_UTR
AAGAGAACACAGGTGAGGAT+TGG 0.524366 5_4:-21143181 Msa0843490:CDS
TTGACAGATGAAGTATTACA+AGG 0.554955 5_4:-21143548 Msa0843490:CDS
TCTTAAGATATTGAATATTG+CGG 0.559358 5_4:-21143516 Msa0843490:CDS
ATGGCAATGGCCTACGTAGG+AGG 0.564493 5_4:-21143116 Msa0843490:CDS
TGAAGTTGGGCCTACACGAA+GGG 0.569134 5_4:-21142453 Msa0843490:three_prime_UTR
CTTCATCTGTCAATGACTTG+AGG 0.571457 5_4:+21143558 None:intergenic
GGTTTCCTAACCAGTCAGGT+AGG 0.573605 5_4:+21143333 None:intergenic
ATTGAGTCATCTTCCAAACC+TGG 0.589554 5_4:-21143360 Msa0843490:CDS
TAAATCCGCGTGTCTTAGCA+AGG 0.593666 5_4:+21142408 None:intergenic
GTTTCCTAACCAGTCAGGTA+GGG 0.595827 5_4:+21143334 None:intergenic
TAAGGTTACTGATTTCGGCT+GGG 0.597787 5_4:+21143618 None:intergenic
CTGAAGTTGGGCCTACACGA+AGG 0.602110 5_4:-21142454 Msa0843490:three_prime_UTR
TTATGGTTGCAGTGAGTTAG+GGG 0.607014 5_4:-21143213 Msa0843490:intron
AAAACCTCTTCCTCCTACGT+AGG 0.613710 5_4:+21143106 None:intergenic
TGTACAAAACAAAATGGCAA+TGG 0.629821 5_4:-21143129 Msa0843490:CDS
TTAAGATATTGAATATTGCG+GGG 0.636428 5_4:-21143514 Msa0843490:CDS
AGGTAGAATCCCCTTCGTGT+AGG 0.645544 5_4:+21142443 None:intergenic
GAAGTTGGGCCTACACGAAG+GGG 0.647022 5_4:-21142452 Msa0843490:three_prime_UTR
TGACAGATGAAGTATTACAA+GGG 0.675775 5_4:-21143547 Msa0843490:CDS
AAAATGGCAATGGCCTACGT+AGG 0.683299 5_4:-21143119 Msa0843490:CDS
ATGTAAAGAGAACACAGGTG+AGG 0.694802 5_4:-21143186 Msa0843490:CDS
TTTATCCTTGCTAAGACACG+CGG 0.699753 5_4:-21142413 Msa0843490:three_prime_UTR
GAACGATGTAAAGAGAACAC+AGG 0.739326 5_4:-21143191 Msa0843490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAAAATTTTGAGAATTTA+TGG - chr5_4:21142709-21142728 Msa0843490:intron 15.0%
!!! TATTTTCAACTATTATTACA+TGG + chr5_4:21143274-21143293 None:intergenic 15.0%
!!! CTTAAAATTTTGAGAATTTA+TGG - chr5_4:21142709-21142728 Msa0843490:intron 15.0%
!!! TATTTTCAACTATTATTACA+TGG + chr5_4:21143274-21143293 None:intergenic 15.0%
!!! AATTTGTGATCATTATTTCT+TGG + chr5_4:21142354-21142373 None:intergenic 20.0%
!! TCTTAAGATATTGAATATTG+CGG - chr5_4:21142423-21142442 Msa0843490:three_prime_UTR 20.0%
!!! TATTGAATGTGAGTTATTTT+TGG + chr5_4:21142784-21142803 None:intergenic 20.0%
!!! TATCAATGTTCTTTTTTCTT+TGG + chr5_4:21143114-21143133 None:intergenic 20.0%
!! AAAGAAAAAAGAACATTGAT+AGG - chr5_4:21143113-21143132 Msa0843490:CDS 20.0%
!! AAGAAAAAAGAACATTGATA+GGG - chr5_4:21143114-21143133 Msa0843490:CDS 20.0%
!! AAAATGATAAAACTTAAGTG+AGG + chr5_4:21143374-21143393 None:intergenic 20.0%
!!! AATTTGTGATCATTATTTCT+TGG + chr5_4:21142354-21142373 None:intergenic 20.0%
!! TCTTAAGATATTGAATATTG+CGG - chr5_4:21142423-21142442 Msa0843490:three_prime_UTR 20.0%
!!! TATTGAATGTGAGTTATTTT+TGG + chr5_4:21142784-21142803 None:intergenic 20.0%
!!! TATCAATGTTCTTTTTTCTT+TGG + chr5_4:21143114-21143133 None:intergenic 20.0%
!! AAAGAAAAAAGAACATTGAT+AGG - chr5_4:21143113-21143132 Msa0843490:CDS 20.0%
!! AAGAAAAAAGAACATTGATA+GGG - chr5_4:21143114-21143133 Msa0843490:CDS 20.0%
!! AAAATGATAAAACTTAAGTG+AGG + chr5_4:21143374-21143393 None:intergenic 20.0%
! ACAAATGTCAAAAACCTTTA+GGG + chr5_4:21142278-21142297 None:intergenic 25.0%
!! TTATTTCTTGGATAACAGTA+AGG + chr5_4:21142342-21142361 None:intergenic 25.0%
! CTTAAGATATTGAATATTGC+GGG - chr5_4:21142424-21142443 Msa0843490:three_prime_UTR 25.0%
! TTAAGATATTGAATATTGCG+GGG - chr5_4:21142425-21142444 Msa0843490:three_prime_UTR 25.0%
!!! CTTCTATTTCTGAAGAATTT+TGG + chr5_4:21142513-21142532 None:intergenic 25.0%
! CAAAATTCTTCAGAAATAGA+AGG - chr5_4:21142511-21142530 Msa0843490:intron 25.0%
!!! TCAAAATTTTAAGACCAGTA+AGG + chr5_4:21142702-21142721 None:intergenic 25.0%
! CATTGATGTACAAAACAAAA+TGG - chr5_4:21142804-21142823 Msa0843490:intron 25.0%
! TTACAACTAAGTAAAGAAGT+TGG - chr5_4:21142978-21142997 Msa0843490:intron 25.0%
! TACAACTAAGTAAAGAAGTT+GGG - chr5_4:21142979-21142998 Msa0843490:intron 25.0%
! ACAGGATACAATTAATTAGA+AGG + chr5_4:21143421-21143440 None:intergenic 25.0%
! TTCTAATTAATTGTATCCTG+TGG - chr5_4:21143420-21143439 Msa0843490:CDS 25.0%
! AGATTGAATGTATCTTAGTT+AGG - chr5_4:21143574-21143593 Msa0843490:CDS 25.0%
! ACAAAACTTCGATTTAATCA+AGG - chr5_4:21143662-21143681 Msa0843490:CDS 25.0%
! ACAAATGTCAAAAACCTTTA+GGG + chr5_4:21142278-21142297 None:intergenic 25.0%
!! TTATTTCTTGGATAACAGTA+AGG + chr5_4:21142342-21142361 None:intergenic 25.0%
! CTTAAGATATTGAATATTGC+GGG - chr5_4:21142424-21142443 Msa0843490:three_prime_UTR 25.0%
! TTAAGATATTGAATATTGCG+GGG - chr5_4:21142425-21142444 Msa0843490:three_prime_UTR 25.0%
!!! CTTCTATTTCTGAAGAATTT+TGG + chr5_4:21142513-21142532 None:intergenic 25.0%
! CAAAATTCTTCAGAAATAGA+AGG - chr5_4:21142511-21142530 Msa0843490:intron 25.0%
!!! TCAAAATTTTAAGACCAGTA+AGG + chr5_4:21142702-21142721 None:intergenic 25.0%
! CATTGATGTACAAAACAAAA+TGG - chr5_4:21142804-21142823 Msa0843490:intron 25.0%
! TTACAACTAAGTAAAGAAGT+TGG - chr5_4:21142978-21142997 Msa0843490:intron 25.0%
! TACAACTAAGTAAAGAAGTT+GGG - chr5_4:21142979-21142998 Msa0843490:intron 25.0%
! ACAGGATACAATTAATTAGA+AGG + chr5_4:21143421-21143440 None:intergenic 25.0%
! TTCTAATTAATTGTATCCTG+TGG - chr5_4:21143420-21143439 Msa0843490:CDS 25.0%
! AGATTGAATGTATCTTAGTT+AGG - chr5_4:21143574-21143593 Msa0843490:CDS 25.0%
! ACAAAACTTCGATTTAATCA+AGG - chr5_4:21143662-21143681 Msa0843490:CDS 25.0%
CACAAATGTCAAAAACCTTT+AGG + chr5_4:21142279-21142298 None:intergenic 30.0%
!! AACAGTAAGGTTACTGATTT+CGG + chr5_4:21142329-21142348 None:intergenic 30.0%
! TTGACAGATGAAGTATTACA+AGG - chr5_4:21142391-21142410 Msa0843490:three_prime_UTR 30.0%
! TGACAGATGAAGTATTACAA+GGG - chr5_4:21142392-21142411 Msa0843490:three_prime_UTR 30.0%
! AGAAATAGAAGGTTTTCATG+AGG - chr5_4:21142522-21142541 Msa0843490:intron 30.0%
! GAAATAGAAGGTTTTCATGA+GGG - chr5_4:21142523-21142542 Msa0843490:intron 30.0%
! CTATAATTTGAAGCAAACCT+AGG + chr5_4:21142630-21142649 None:intergenic 30.0%
TGTACAAAACAAAATGGCAA+TGG - chr5_4:21142810-21142829 Msa0843490:intron 30.0%
CAAGACAAAAACATCATATG+TGG + chr5_4:21142932-21142951 None:intergenic 30.0%
!! CACATATGATGTTTTTGTCT+TGG - chr5_4:21142930-21142949 Msa0843490:intron 30.0%
TTTCAAATTGTAAGCTCAAC+TGG + chr5_4:21143031-21143050 None:intergenic 30.0%
!! ATAAGCTTTTGAAGAGATTC+TGG - chr5_4:21143144-21143163 Msa0843490:CDS 30.0%
!! TAAGCTTTTGAAGAGATTCT+GGG - chr5_4:21143145-21143164 Msa0843490:CDS 30.0%
TTGGAATCTACAAAAGCAAA+AGG + chr5_4:21143213-21143232 None:intergenic 30.0%
CAACTATTATTACATGGTTG+AGG + chr5_4:21143268-21143287 None:intergenic 30.0%
AATGCATGACATTACTGTAA+TGG - chr5_4:21143324-21143343 Msa0843490:intron 30.0%
GAAAATATATCCGTGAACTA+AGG - chr5_4:21143346-21143365 Msa0843490:CDS 30.0%
!! GCGGATTTAATTTGATATGA+GGG - chr5_4:21143545-21143564 Msa0843490:CDS 30.0%
! TGTTTTCTCACTACTTCTAT+CGG - chr5_4:21143632-21143651 Msa0843490:CDS 30.0%
CACAAATGTCAAAAACCTTT+AGG + chr5_4:21142279-21142298 None:intergenic 30.0%
!! AACAGTAAGGTTACTGATTT+CGG + chr5_4:21142329-21142348 None:intergenic 30.0%
! TTGACAGATGAAGTATTACA+AGG - chr5_4:21142391-21142410 Msa0843490:three_prime_UTR 30.0%
! TGACAGATGAAGTATTACAA+GGG - chr5_4:21142392-21142411 Msa0843490:three_prime_UTR 30.0%
! AGAAATAGAAGGTTTTCATG+AGG - chr5_4:21142522-21142541 Msa0843490:intron 30.0%
! GAAATAGAAGGTTTTCATGA+GGG - chr5_4:21142523-21142542 Msa0843490:intron 30.0%
! CTATAATTTGAAGCAAACCT+AGG + chr5_4:21142630-21142649 None:intergenic 30.0%
TGTACAAAACAAAATGGCAA+TGG - chr5_4:21142810-21142829 Msa0843490:intron 30.0%
CAAGACAAAAACATCATATG+TGG + chr5_4:21142932-21142951 None:intergenic 30.0%
!! CACATATGATGTTTTTGTCT+TGG - chr5_4:21142930-21142949 Msa0843490:intron 30.0%
TTTCAAATTGTAAGCTCAAC+TGG + chr5_4:21143031-21143050 None:intergenic 30.0%
!! ATAAGCTTTTGAAGAGATTC+TGG - chr5_4:21143144-21143163 Msa0843490:CDS 30.0%
!! TAAGCTTTTGAAGAGATTCT+GGG - chr5_4:21143145-21143164 Msa0843490:CDS 30.0%
TTGGAATCTACAAAAGCAAA+AGG + chr5_4:21143213-21143232 None:intergenic 30.0%
CAACTATTATTACATGGTTG+AGG + chr5_4:21143268-21143287 None:intergenic 30.0%
AATGCATGACATTACTGTAA+TGG - chr5_4:21143324-21143343 Msa0843490:intron 30.0%
GAAAATATATCCGTGAACTA+AGG - chr5_4:21143346-21143365 Msa0843490:CDS 30.0%
!! GCGGATTTAATTTGATATGA+GGG - chr5_4:21143545-21143564 Msa0843490:CDS 30.0%
! TGTTTTCTCACTACTTCTAT+CGG - chr5_4:21143632-21143651 Msa0843490:CDS 30.0%
! CAAAAACCTTTAGGGAAGTA+AGG + chr5_4:21142270-21142289 None:intergenic 35.0%
ATTTATGGTTGCAGTGAGTT+AGG - chr5_4:21142724-21142743 Msa0843490:intron 35.0%
TTTATGGTTGCAGTGAGTTA+GGG - chr5_4:21142725-21142744 Msa0843490:intron 35.0%
AATATAGTCCCTGCTGTTAT+CGG - chr5_4:21143175-21143194 Msa0843490:CDS 35.0%
TAGCAAGGATAAACTGTTGA+AGG + chr5_4:21143519-21143538 None:intergenic 35.0%
!! CGCGGATTTAATTTGATATG+AGG - chr5_4:21143544-21143563 Msa0843490:CDS 35.0%
! CAAAAACCTTTAGGGAAGTA+AGG + chr5_4:21142270-21142289 None:intergenic 35.0%
ATTTATGGTTGCAGTGAGTT+AGG - chr5_4:21142724-21142743 Msa0843490:intron 35.0%
TTTATGGTTGCAGTGAGTTA+GGG - chr5_4:21142725-21142744 Msa0843490:intron 35.0%
AATATAGTCCCTGCTGTTAT+CGG - chr5_4:21143175-21143194 Msa0843490:CDS 35.0%
TAGCAAGGATAAACTGTTGA+AGG + chr5_4:21143519-21143538 None:intergenic 35.0%
!! CGCGGATTTAATTTGATATG+AGG - chr5_4:21143544-21143563 Msa0843490:CDS 35.0%
ACGAAACCTTACTTCCCTAA+AGG - chr5_4:21142261-21142280 Msa0843490:three_prime_UTR 40.0%
!! TAAGGTTACTGATTTCGGCT+GGG + chr5_4:21142324-21142343 None:intergenic 40.0%
CTTCATCTGTCAATGACTTG+AGG + chr5_4:21142384-21142403 None:intergenic 40.0%
ATTGAGTCATCTTCCAAACC+TGG - chr5_4:21142579-21142598 Msa0843490:intron 40.0%
!! GTAAGGCATTGAATGCTCAT+GGG + chr5_4:21142685-21142704 None:intergenic 40.0%
!! AGTAAGGCATTGAATGCTCA+TGG + chr5_4:21142686-21142705 None:intergenic 40.0%
ATGAGCATTCAATGCCTTAC+TGG - chr5_4:21142685-21142704 Msa0843490:intron 40.0%
TTATGGTTGCAGTGAGTTAG+GGG - chr5_4:21142726-21142745 Msa0843490:intron 40.0%
GAACGATGTAAAGAGAACAC+AGG - chr5_4:21142748-21142767 Msa0843490:intron 40.0%
ATGTAAAGAGAACACAGGTG+AGG - chr5_4:21142753-21142772 Msa0843490:intron 40.0%
! GTAGGAGGAAGAGGTTTTTA+TGG - chr5_4:21142838-21142857 Msa0843490:intron 40.0%
CTGGAATAAGAAGACAGTCT+TGG + chr5_4:21143012-21143031 None:intergenic 40.0%
AAATGCATCTTCACGATGCT+TGG + chr5_4:21143232-21143251 None:intergenic 40.0%
AGCATCGTGAAGATGCATTT+AGG - chr5_4:21143232-21143251 Msa0843490:intron 40.0%
AAGTGAGGTTCCTTAGTTCA+CGG + chr5_4:21143359-21143378 None:intergenic 40.0%
! CTTCTCAATGCTGTTTGAAG+TGG - chr5_4:21143457-21143476 Msa0843490:CDS 40.0%
TTTATCCTTGCTAAGACACG+CGG - chr5_4:21143526-21143545 Msa0843490:CDS 40.0%
ACGAAACCTTACTTCCCTAA+AGG - chr5_4:21142261-21142280 Msa0843490:three_prime_UTR 40.0%
!! TAAGGTTACTGATTTCGGCT+GGG + chr5_4:21142324-21142343 None:intergenic 40.0%
CTTCATCTGTCAATGACTTG+AGG + chr5_4:21142384-21142403 None:intergenic 40.0%
ATTGAGTCATCTTCCAAACC+TGG - chr5_4:21142579-21142598 Msa0843490:intron 40.0%
!! GTAAGGCATTGAATGCTCAT+GGG + chr5_4:21142685-21142704 None:intergenic 40.0%
!! AGTAAGGCATTGAATGCTCA+TGG + chr5_4:21142686-21142705 None:intergenic 40.0%
ATGAGCATTCAATGCCTTAC+TGG - chr5_4:21142685-21142704 Msa0843490:intron 40.0%
TTATGGTTGCAGTGAGTTAG+GGG - chr5_4:21142726-21142745 Msa0843490:intron 40.0%
GAACGATGTAAAGAGAACAC+AGG - chr5_4:21142748-21142767 Msa0843490:intron 40.0%
ATGTAAAGAGAACACAGGTG+AGG - chr5_4:21142753-21142772 Msa0843490:intron 40.0%
! GTAGGAGGAAGAGGTTTTTA+TGG - chr5_4:21142838-21142857 Msa0843490:intron 40.0%
CTGGAATAAGAAGACAGTCT+TGG + chr5_4:21143012-21143031 None:intergenic 40.0%
AAATGCATCTTCACGATGCT+TGG + chr5_4:21143232-21143251 None:intergenic 40.0%
AGCATCGTGAAGATGCATTT+AGG - chr5_4:21143232-21143251 Msa0843490:intron 40.0%
AAGTGAGGTTCCTTAGTTCA+CGG + chr5_4:21143359-21143378 None:intergenic 40.0%
! CTTCTCAATGCTGTTTGAAG+TGG - chr5_4:21143457-21143476 Msa0843490:CDS 40.0%
TTTATCCTTGCTAAGACACG+CGG - chr5_4:21143526-21143545 Msa0843490:CDS 40.0%
!! GTAAGGTTACTGATTTCGGC+TGG + chr5_4:21142325-21142344 None:intergenic 45.0%
GTTTCCTAACCAGTCAGGTA+GGG + chr5_4:21142608-21142627 None:intergenic 45.0%
AAGAGAACACAGGTGAGGAT+TGG - chr5_4:21142758-21142777 Msa0843490:intron 45.0%
AAAATGGCAATGGCCTACGT+AGG - chr5_4:21142820-21142839 Msa0843490:intron 45.0%
AAAACCTCTTCCTCCTACGT+AGG + chr5_4:21142836-21142855 None:intergenic 45.0%
TCTTGCTTCCGATAACAGCA+GGG + chr5_4:21143186-21143205 None:intergenic 45.0%
TGAAGTGGTTCTCCTGAAGT+TGG - chr5_4:21143472-21143491 Msa0843490:CDS 45.0%
GAAGTGGTTCTCCTGAAGTT+GGG - chr5_4:21143473-21143492 Msa0843490:CDS 45.0%
TAAATCCGCGTGTCTTAGCA+AGG + chr5_4:21143534-21143553 None:intergenic 45.0%
!! GTAAGGTTACTGATTTCGGC+TGG + chr5_4:21142325-21142344 None:intergenic 45.0%
GTTTCCTAACCAGTCAGGTA+GGG + chr5_4:21142608-21142627 None:intergenic 45.0%
AAGAGAACACAGGTGAGGAT+TGG - chr5_4:21142758-21142777 Msa0843490:intron 45.0%
AAAATGGCAATGGCCTACGT+AGG - chr5_4:21142820-21142839 Msa0843490:intron 45.0%
AAAACCTCTTCCTCCTACGT+AGG + chr5_4:21142836-21142855 None:intergenic 45.0%
TCTTGCTTCCGATAACAGCA+GGG + chr5_4:21143186-21143205 None:intergenic 45.0%
TGAAGTGGTTCTCCTGAAGT+TGG - chr5_4:21143472-21143491 Msa0843490:CDS 45.0%
GAAGTGGTTCTCCTGAAGTT+GGG - chr5_4:21143473-21143492 Msa0843490:CDS 45.0%
TAAATCCGCGTGTCTTAGCA+AGG + chr5_4:21143534-21143553 None:intergenic 45.0%
GGTTTCCTAACCAGTCAGGT+AGG + chr5_4:21142609-21142628 None:intergenic 50.0%
CCTAGGTTTCCTAACCAGTC+AGG + chr5_4:21142613-21142632 None:intergenic 50.0%
! CCTGACTGGTTAGGAAACCT+AGG - chr5_4:21142610-21142629 Msa0843490:intron 50.0%
! GGCATTGAATGCTCATGGGA+AGG + chr5_4:21142681-21142700 None:intergenic 50.0%
AAGTGCACTCGTCATTGGAC+AGG + chr5_4:21143081-21143100 None:intergenic 50.0%
TGTCCAATGACGAGTGCACT+TGG - chr5_4:21143080-21143099 Msa0843490:intron 50.0%
TCACCAAGTGCACTCGTCAT+TGG + chr5_4:21143086-21143105 None:intergenic 50.0%
CTCTTGCTTCCGATAACAGC+AGG + chr5_4:21143187-21143206 None:intergenic 50.0%
CTGCTGTTATCGGAAGCAAG+AGG - chr5_4:21143185-21143204 Msa0843490:CDS 50.0%
TGAAGTTGGGCCTACACGAA+GGG - chr5_4:21143486-21143505 Msa0843490:CDS 50.0%
AGGTAGAATCCCCTTCGTGT+AGG + chr5_4:21143499-21143518 None:intergenic 50.0%
GGTTTCCTAACCAGTCAGGT+AGG + chr5_4:21142609-21142628 None:intergenic 50.0%
CCTAGGTTTCCTAACCAGTC+AGG + chr5_4:21142613-21142632 None:intergenic 50.0%
! CCTGACTGGTTAGGAAACCT+AGG - chr5_4:21142610-21142629 Msa0843490:intron 50.0%
! GGCATTGAATGCTCATGGGA+AGG + chr5_4:21142681-21142700 None:intergenic 50.0%
AAGTGCACTCGTCATTGGAC+AGG + chr5_4:21143081-21143100 None:intergenic 50.0%
TGTCCAATGACGAGTGCACT+TGG - chr5_4:21143080-21143099 Msa0843490:intron 50.0%
TCACCAAGTGCACTCGTCAT+TGG + chr5_4:21143086-21143105 None:intergenic 50.0%
CTCTTGCTTCCGATAACAGC+AGG + chr5_4:21143187-21143206 None:intergenic 50.0%
CTGCTGTTATCGGAAGCAAG+AGG - chr5_4:21143185-21143204 Msa0843490:CDS 50.0%
TGAAGTTGGGCCTACACGAA+GGG - chr5_4:21143486-21143505 Msa0843490:CDS 50.0%
AGGTAGAATCCCCTTCGTGT+AGG + chr5_4:21143499-21143518 None:intergenic 50.0%
TTTCGGCTGGGAGTTGCTCA+AGG + chr5_4:21142312-21142331 None:intergenic 55.0%
TCAGGTAGGGATGCCAGGTT+TGG + chr5_4:21142595-21142614 None:intergenic 55.0%
! GCATCCCTACCTGACTGGTT+AGG - chr5_4:21142601-21142620 Msa0843490:intron 55.0%
! ATGGGAAGGCACGTCAGCTT+TGG + chr5_4:21142667-21142686 None:intergenic 55.0%
ATGGCAATGGCCTACGTAGG+AGG - chr5_4:21142823-21142842 Msa0843490:intron 55.0%
ATGGCCTACGTAGGAGGAAG+AGG - chr5_4:21142829-21142848 Msa0843490:intron 55.0%
AGTTCTGCATCTGCAGCCAC+AGG + chr5_4:21143439-21143458 None:intergenic 55.0%
CTTCGTGTAGGCCCAACTTC+AGG + chr5_4:21143487-21143506 None:intergenic 55.0%
CTGAAGTTGGGCCTACACGA+AGG - chr5_4:21143485-21143504 Msa0843490:CDS 55.0%
GAAGTTGGGCCTACACGAAG+GGG - chr5_4:21143487-21143506 Msa0843490:CDS 55.0%
TTTCGGCTGGGAGTTGCTCA+AGG + chr5_4:21142312-21142331 None:intergenic 55.0%
TCAGGTAGGGATGCCAGGTT+TGG + chr5_4:21142595-21142614 None:intergenic 55.0%
! GCATCCCTACCTGACTGGTT+AGG - chr5_4:21142601-21142620 Msa0843490:intron 55.0%
! ATGGGAAGGCACGTCAGCTT+TGG + chr5_4:21142667-21142686 None:intergenic 55.0%
ATGGCAATGGCCTACGTAGG+AGG - chr5_4:21142823-21142842 Msa0843490:intron 55.0%
ATGGCCTACGTAGGAGGAAG+AGG - chr5_4:21142829-21142848 Msa0843490:intron 55.0%
AGTTCTGCATCTGCAGCCAC+AGG + chr5_4:21143439-21143458 None:intergenic 55.0%
CTTCGTGTAGGCCCAACTTC+AGG + chr5_4:21143487-21143506 None:intergenic 55.0%
CTGAAGTTGGGCCTACACGA+AGG - chr5_4:21143485-21143504 Msa0843490:CDS 55.0%
GAAGTTGGGCCTACACGAAG+GGG - chr5_4:21143487-21143506 Msa0843490:CDS 55.0%
GGCTGGGAGTTGCTCAAGGT+TGG + chr5_4:21142308-21142327 None:intergenic 60.0%
ACCTGGCATCCCTACCTGAC+TGG - chr5_4:21142596-21142615 Msa0843490:intron 60.0%
ACCAGTCAGGTAGGGATGCC+AGG + chr5_4:21142600-21142619 None:intergenic 60.0%
GGCTGGGAGTTGCTCAAGGT+TGG + chr5_4:21142308-21142327 None:intergenic 60.0%
ACCTGGCATCCCTACCTGAC+TGG - chr5_4:21142596-21142615 Msa0843490:intron 60.0%
ACCAGTCAGGTAGGGATGCC+AGG + chr5_4:21142600-21142619 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 21142256 21143705 21142256 ID=Msa0843490;Name=Msa0843490
chr5_4 mRNA 21142256 21143705 21142256 ID=Msa0843490-mRNA-1;Parent=Msa0843490;Name=Msa0843490-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0|0.33|1|0|0|3|231|166
chr5_4 exon 21142256 21142512 21142256 ID=Msa0843490-mRNA-1:exon:8783;Parent=Msa0843490-mRNA-1
chr5_4 exon 21143111 21143223 21143111 ID=Msa0843490-mRNA-1:exon:8782;Parent=Msa0843490-mRNA-1
chr5_4 exon 21143344 21143705 21143344 ID=Msa0843490-mRNA-1:exon:8781;Parent=Msa0843490-mRNA-1
chr5_4 CDS 21143344 21143705 21143344 ID=Msa0843490-mRNA-1:cds;Parent=Msa0843490-mRNA-1
chr5_4 CDS 21143111 21143223 21143111 ID=Msa0843490-mRNA-1:cds;Parent=Msa0843490-mRNA-1
chr5_4 CDS 21142487 21142512 21142487 ID=Msa0843490-mRNA-1:cds;Parent=Msa0843490-mRNA-1
chr5_4 three_prime_UTR 21142256 21142486 21142256 ID=Msa0843490-mRNA-1:three_prime_utr;Parent=Msa0843490-mRNA-1
Gene Sequence

>Msa0843490

ATGCTACGAAACCTTACTTCCCTAAAGGTTTTTGACATTTGTGAGCTTTCCAACCTTGAGCAACTCCCAGCCGAAATCAGTAACCTTACTGTTATCCAAGAAATAATGATCACAAATTGCAAGAACCTCAAGTCATTGACAGATGAAGTATTACAAGGGTTGCACTCTCTTAAGATATTGAATATTGCGGGGTGCAAAAAGTTCAATCTGTCAGAAAGTTTTAAATATCTCACTTGTCTTGAGAAATTGATGATCCAAAATTCTTCAGAAATAGAAGGTTTTCATGAGGGTTTACAACATATGACTGCTCTTAAATCTTTGATATTGAGTCATCTTCCAAACCTGGCATCCCTACCTGACTGTGAGTTAGGGGAACGATGTAAAGAGAACACAGGTGAGGATTGGCCAAAAATAACTCACATTCAATACATTGATGTACAAAACAAAATGGCAATGGCCTACGTAGGAGGAAGAGATGCAGAACTTCTCAATGCTGTTTGA
Protein sequence

>Msa0843490

MLRNLTSLKVFDICELSNLEQLPAEISNLTVIQEIMITNCKNLKSLTDEVLQGLHSLKILNIAGCKKFNLSESFKYLTCLEKLMIQNSSEIEGFHEGLQHMTALKSLILSHLPNLASLPDCELGERCKENTGEDWPKITHIQYIDVQNKMAMAYVGGRDAELLNAV*