AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0847640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0847640 MtrunA17_Chr2g0330011 88.710 124 14 0 68 191 1 124 4.01e-72 222
Msa0847640 MtrunA17_Chr4g0073341 56.923 65 14 3 2 66 90 140 4.26e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 139 sgRNAs with CRISPR-Local

Find 342 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATACAGCATTGAGAAAA+TGG 0.197334 5_4:-30098990 Msa0847640:intron
CATGCCGAAGCCCTTGATTT+TGG 0.246438 5_4:-30095480 Msa0847640:intron
TGCATCTGCACACCATTTAC+TGG 0.270167 5_4:-30095298 Msa0847640:intron
GAGTGGGTGTCATAAGATTT+TGG 0.274189 5_4:-30099252 Msa0847640:CDS
AAATTTATGCAGGGAATAAT+AGG 0.279676 5_4:-30097265 Msa0847640:intron
GCCTTGAGCAAGTTGAATTC+TGG 0.280287 5_4:-30099380 Msa0847640:intron
TATTTCTCCCATCGTCATTA+AGG 0.285243 5_4:+30099178 None:intergenic
CCGTATAAAGATATCCTATA+TGG 0.308033 5_4:-30094235 Msa0847640:three_prime_UTR
CAGAACACACATGGAAATAA+TGG 0.315521 5_4:-30097721 Msa0847640:three_prime_UTR
TCATCTTGTTACAATTTCAT+AGG 0.315939 5_4:+30094106 None:intergenic
AATGCTCTAATCACGGATTC+AGG 0.338266 5_4:+30097205 None:intergenic
TGTCTAACAATACCAGTAAA+TGG 0.338321 5_4:+30095286 None:intergenic
GCCGATAGAAATTTGTTGTT+TGG 0.341277 5_4:-30094083 None:intergenic
AGGAGATAAAAGTTATGTTT+AGG 0.344717 5_4:-30098154 Msa0847640:three_prime_UTR
CCATATAGGATATCTTTATA+CGG 0.354474 5_4:+30094235 None:intergenic
TCCTGGTGCTTAGTCCTTTG+AGG 0.367972 5_4:-30096011 Msa0847640:intron
TCTTGATCAAGTTAAGGCTT+TGG 0.372096 5_4:-30094577 Msa0847640:three_prime_UTR
GGTGAAGTTGATTTCAACTT+TGG 0.376419 5_4:-30097574 Msa0847640:three_prime_UTR
TTATTACCTCAAAATAAGAT+TGG 0.382454 5_4:+30097545 None:intergenic
CCTTTCCGACCTCTGGTGCT+TGG 0.384030 5_4:+30095771 None:intergenic
TATAAGAATTGTTATATTCA+AGG 0.384278 5_4:-30097595 Msa0847640:three_prime_UTR
GCAGTAGCTGCTACTATAAT+CGG 0.396291 5_4:-30097694 Msa0847640:three_prime_UTR
TCATCATCGCCGAAGGAAAC+CGG 0.400578 5_4:+30095822 None:intergenic
CAACAATGATACTAAACTAT+TGG 0.402664 5_4:-30095179 Msa0847640:three_prime_UTR
TAGTGTAATTACTGTTTCTT+CGG 0.411676 5_4:-30099453 Msa0847640:CDS
TTCCTTTCATGAAAGGTCTC+TGG 0.412565 5_4:+30094637 None:intergenic
AATTATAAAATTAACCATAT+AGG 0.417706 5_4:+30094221 None:intergenic
ATGAAAGGTCTCTGGTACAT+TGG 0.419417 5_4:+30094645 None:intergenic
AACAGATCCTTTCCGACCTC+TGG 0.421775 5_4:+30095764 None:intergenic
TATTACCTCAAAATAAGATT+GGG 0.423552 5_4:+30097546 None:intergenic
GTGCTAAGCAGTAATGAGTT+TGG 0.430118 5_4:-30094377 Msa0847640:three_prime_UTR
AGGATCATAAGAGGCTTTCT+CGG 0.436457 5_4:+30097225 None:intergenic
AGACACTTTGTGGGATGCAT+TGG 0.439352 5_4:-30097629 Msa0847640:three_prime_UTR
AAGGCAAAACAACAATTAGA+TGG 0.440636 5_4:-30098039 Msa0847640:three_prime_UTR
CTGTGGAAGCATAATGTCAA+TGG 0.445454 5_4:-30095336 Msa0847640:three_prime_UTR
CTGGTCATGCAAGCCTTCAT+AGG 0.446151 5_4:+30098828 None:intergenic
GGAGCGTTCGACGCGCTTCA+AGG 0.449202 5_4:-30096050 Msa0847640:three_prime_UTR
GATAAAGCATACCAAAATCA+AGG 0.451714 5_4:+30095469 None:intergenic
GGGTTATCAAGACACTTTGT+GGG 0.453972 5_4:-30097638 Msa0847640:three_prime_UTR
CAGAAAGTGCCTGCACTCTC+AGG 0.454731 5_4:+30098189 None:intergenic
GACACTTTGTGGGATGCATT+GGG 0.456315 5_4:-30097628 Msa0847640:three_prime_UTR
GCTGATAGTCCGGTTTCCTT+CGG 0.463519 5_4:-30095831 Msa0847640:three_prime_UTR
TGTTGTTGGTCGAGATAGAA+AGG 0.466181 5_4:-30099144 Msa0847640:intron
ATTTACTCTCTTCTTCGCTA+TGG 0.472481 5_4:-30095603 Msa0847640:three_prime_UTR
TCTTCGGACAAAGGTATTTG+TGG 0.478458 5_4:-30099437 Msa0847640:CDS
TCATTAAGGAGTGAACTTGC+TGG 0.481303 5_4:+30099192 None:intergenic
TCTGTCGTGCCTGGTTTCAA+TGG 0.481540 5_4:+30095121 None:intergenic
CACGGATTCAGGATCATAAG+AGG 0.485361 5_4:+30097216 None:intergenic
TGCAGCTGGAGCTGATAGTC+CGG 0.486818 5_4:-30095841 Msa0847640:three_prime_UTR
AAGCAACCTAGAGTTGCAAC+AGG 0.491647 5_4:-30098754 Msa0847640:CDS
TTTGTTCTAGGAACGTGTTA+AGG 0.492658 5_4:-30096071 Msa0847640:intron
GGCCTACCTGTTGCAACTCT+AGG 0.493031 5_4:+30098748 None:intergenic
CCACGTCTGTCAAGATAAGC+AGG 0.495862 5_4:-30099403 Msa0847640:CDS
ATGTGCTGTGGTAGAACCTT+TGG 0.500206 5_4:-30094352 Msa0847640:three_prime_UTR
TGAGTCTCTTGATCAAGTTA+AGG 0.500572 5_4:-30094583 Msa0847640:three_prime_UTR
TGGGTTATCAAGACACTTTG+TGG 0.502212 5_4:-30097639 Msa0847640:three_prime_UTR
CACAAAGAGATAATTAAGAG+TGG 0.502433 5_4:-30099269 Msa0847640:CDS
ATAGCGCGTGGAGTTGTTGT+TGG 0.503009 5_4:-30099158 Msa0847640:CDS
CTTTGTTGGGCTAAACATAG+TGG 0.503747 5_4:-30094303 Msa0847640:three_prime_UTR
TTTCAAGGCAGAAGTGCAGC+TGG 0.505124 5_4:-30095855 Msa0847640:three_prime_UTR
TTAACTTGATCAAGAGACTC+AGG 0.506424 5_4:+30094585 None:intergenic
CATACCAAAATCAAGGGCTT+CGG 0.509220 5_4:+30095476 None:intergenic
AGAGGATGCATTTAGTATTG+AGG 0.509968 5_4:-30098807 Msa0847640:CDS
CCTCTGTCGTGAAGTTACTA+AGG 0.511005 5_4:-30095511 Msa0847640:three_prime_UTR
CAATGATACTAAACTATTGG+TGG 0.511697 5_4:-30095176 Msa0847640:three_prime_UTR
ATTTGTTGGGTTTACCTCAA+AGG 0.513130 5_4:+30095997 None:intergenic
ACTTGGTCGGCAAGTAACTC+AGG 0.515239 5_4:+30098727 None:intergenic
GTATTAATCCCTTACTTGGT+CGG 0.518534 5_4:+30098714 None:intergenic
TACAAGGCAATCAATGATGA+AGG 0.521364 5_4:-30094672 Msa0847640:three_prime_UTR
AACTGTGATACAGACCTTGC+TGG 0.524309 5_4:+30094543 None:intergenic
ATATTGTTCATATGCTAAGT+AGG 0.534827 5_4:-30098855 Msa0847640:CDS
GGTTATTGCAGATTCTTACA+AGG 0.535096 5_4:-30094688 Msa0847640:intron
TCAATACTAAATGCATCCTC+TGG 0.535882 5_4:+30098809 None:intergenic
AAAACTATATCTGTCGTGCC+TGG 0.541359 5_4:+30095112 None:intergenic
ACCTCAAAGGACTAAGCACC+AGG 0.545970 5_4:+30096010 None:intergenic
TGGAGACAATTCTGCCAGCA+AGG 0.546425 5_4:-30094557 Msa0847640:three_prime_UTR
CTAAGTAGGATGTCCTATGA+AGG 0.550527 5_4:-30098841 Msa0847640:CDS
ACCAAACAACAAATTTCTAT+CGG 0.551338 5_4:+30094082 None:intergenic
AGGATGAAAGAAATTGCTCA+TGG 0.553009 5_4:-30098787 Msa0847640:CDS
GAATCTTCACAGTTCACTCC+TGG 0.554707 5_4:-30096028 Msa0847640:three_prime_UTR
GAAAATGAAACACGCAAGGA+AGG 0.557148 5_4:+30094128 None:intergenic
ATTACTGTTTCTTCGGACAA+AGG 0.558434 5_4:-30099446 Msa0847640:CDS
TTGAAGAAGAGACAAGAAGA+AGG 0.558667 5_4:-30094467 Msa0847640:three_prime_UTR
TACCAGAGACCTTTCATGAA+AGG 0.560830 5_4:-30094639 Msa0847640:three_prime_UTR
TTTCTCAATGCTGTATTGCA+AGG 0.561099 5_4:+30098994 None:intergenic
TCGGACAAAGGTATTTGTGG+TGG 0.562124 5_4:-30099434 Msa0847640:CDS
ACCAGAATTCAACTTGCTCA+AGG 0.564645 5_4:+30099379 None:intergenic
AAAACAACAATTAGATGGCA+AGG 0.565392 5_4:-30098034 Msa0847640:intron
AGATTGAATTGTGAAGAGTA+TGG 0.566056 5_4:-30100173 Msa0847640:CDS
GCCGTTGTACCTGAGAGTGC+AGG 0.575137 5_4:-30098198 Msa0847640:CDS
ACTTTCAAATGCTCTAATCA+CGG 0.577055 5_4:+30097198 None:intergenic
TGTGGAAGCATAATGTCAAT+GGG 0.578379 5_4:-30095335 Msa0847640:three_prime_UTR
CCAAGCACCAGAGGTCGGAA+AGG 0.579149 5_4:-30095771 Msa0847640:three_prime_UTR
ACAGTTGACAGACATGGAGC+TGG 0.595684 5_4:-30094526 Msa0847640:three_prime_UTR
GGCATCTTCATCATCGCCGA+AGG 0.596640 5_4:+30095815 None:intergenic
AAGCTAAAACACATGCATGA+AGG 0.597709 5_4:+30094323 None:intergenic
ATAAAGCATACCAAAATCAA+GGG 0.599353 5_4:+30095470 None:intergenic
GTTCACTCCTTAATGACGAT+GGG 0.599830 5_4:-30099185 Msa0847640:CDS
ACAAAGAGATAATTAAGAGT+GGG 0.601166 5_4:-30099268 Msa0847640:CDS
AAATACGTGAGCAAGAAAGA+AGG 0.602280 5_4:-30095361 Msa0847640:three_prime_UTR
TAAACAACAAGAAATTGAGA+AGG 0.608713 5_4:-30098058 Msa0847640:three_prime_UTR
TGTACTCTTTGAGCACCACC+AGG 0.609997 5_4:+30094402 None:intergenic
TTAATCTATTTGATGAGCCG+AGG 0.612844 5_4:-30095572 Msa0847640:three_prime_UTR
AGCTCAAAACCAAGCACCAG+AGG 0.612912 5_4:-30095780 Msa0847640:three_prime_UTR
GATGAAGATGATGAGATGCC+TGG 0.615030 5_4:-30094420 Msa0847640:three_prime_UTR
AGAAACTAACAAATCTGCGA+AGG 0.616448 5_4:+30095685 None:intergenic
GGATGAAAGAAATTGCTCAT+GGG 0.619244 5_4:-30098786 Msa0847640:CDS
AGTTCACTCCTTAATGACGA+TGG 0.623286 5_4:-30099186 Msa0847640:CDS
AAGGCAGCAAGCTCAGCAAG+AGG 0.624868 5_4:-30094448 Msa0847640:three_prime_UTR
AGAGAAGACTAATATCGTGA+TGG 0.628417 5_4:+30095724 None:intergenic
TGTATCACAGTTGACAGACA+TGG 0.631121 5_4:-30094532 Msa0847640:three_prime_UTR
CAAAACCAAGCACCAGAGGT+CGG 0.635046 5_4:-30095776 Msa0847640:three_prime_UTR
GGAAGAATACTGATGAGAGG+TGG 0.635557 5_4:+30097411 None:intergenic
ATAGGAAAATGAAACACGCA+AGG 0.638343 5_4:+30094124 None:intergenic
ACTTTACATTAATTTCGCTG+TGG 0.639819 5_4:-30094613 Msa0847640:three_prime_UTR
CACACATGGAAATAATGGAA+AGG 0.640307 5_4:-30097716 Msa0847640:three_prime_UTR
TCTTCCTGACTGCAAAACTG+AGG 0.645676 5_4:-30097394 Msa0847640:intron
GAAGCATAATGTCAATGGGT+TGG 0.646178 5_4:-30095331 Msa0847640:three_prime_UTR
CCTGCTTATCTTGACAGACG+TGG 0.656536 5_4:+30099403 None:intergenic
ATGAGTTTGGAGATGTGCTG+TGG 0.657658 5_4:-30094364 Msa0847640:three_prime_UTR
GTGTAATTGCAGAACACACA+TGG 0.660482 5_4:-30097730 Msa0847640:intron
TGCATGAAGGACATCACCAA+AGG 0.666733 5_4:+30094336 None:intergenic
TCAGGAAGAATACTGATGAG+AGG 0.667501 5_4:+30097408 None:intergenic
CATGGAGCTGGATGAGTGTG+AGG 0.667513 5_4:-30094514 Msa0847640:three_prime_UTR
TAGTAACTTCACGACAGAGG+TGG 0.668692 5_4:+30095514 None:intergenic
ATGAAGGCTTGCATGACCAG+AGG 0.673176 5_4:-30098825 Msa0847640:CDS
CACGTCTGTCAAGATAAGCA+GGG 0.673248 5_4:-30099402 Msa0847640:CDS
ATGCGAAGATTTGACAACCT+CGG 0.681878 5_4:+30095555 None:intergenic
CCTTAGTAACTTCACGACAG+AGG 0.686364 5_4:+30095511 None:intergenic
AATGATTTACCATTGAAACC+AGG 0.687962 5_4:-30095130 Msa0847640:three_prime_UTR
GCCTGCACTCTCAGGTACAA+CGG 0.691524 5_4:+30098197 None:intergenic
TACTGATGAGAGGTGGAGTG+TGG 0.692327 5_4:+30097418 None:intergenic
AACCTAGAGTTGCAACAGGT+AGG 0.693116 5_4:-30098750 Msa0847640:CDS
GAGCAAGAAAGAAGGCTCTG+TGG 0.695391 5_4:-30095353 Msa0847640:three_prime_UTR
TAACTTGATCAAGAGACTCA+GGG 0.698525 5_4:+30094586 None:intergenic
ACGATGGGAGAAATAGCGCG+TGG 0.704553 5_4:-30099170 Msa0847640:CDS
TGGTGTGCAGATGCAACACT+CGG 0.705656 5_4:+30095306 None:intergenic
ACTTTCTGAGAACATACCAG+AGG 0.749148 5_4:-30098174 Msa0847640:exon
GAAGATGATGAGATGCCTGG+TGG 0.783796 5_4:-30094417 Msa0847640:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTATTTATAAGTTTTTA+AGG + chr5_4:30094750-30094769 None:intergenic 10.0%
!! ATGTCAAAATAAATTATTTA+GGG + chr5_4:30094838-30094857 None:intergenic 10.0%
!!! TTTTTTGAAATAAAACAAAT+TGG - chr5_4:30097741-30097760 Msa0847640:intron 10.0%
!! AATTATAAAATTAACCATAT+AGG + chr5_4:30100236-30100255 None:intergenic 10.0%
!!! TTTTTTTTTTGTTGATTTTC+TGG - chr5_4:30094428-30094447 Msa0847640:three_prime_UTR 15.0%
!!! TTTTTTTTTGTTGATTTTCT+GGG - chr5_4:30094429-30094448 Msa0847640:three_prime_UTR 15.0%
!! CATGTCAAAATAAATTATTT+AGG + chr5_4:30094839-30094858 None:intergenic 15.0%
!!! AAATAAACATATCTTTTCAT+AGG + chr5_4:30094957-30094976 None:intergenic 15.0%
!! ATGTTTATTTATAGTGATTA+TGG - chr5_4:30094967-30094986 Msa0847640:intron 15.0%
!!! GAAACTAAATTAACTTTTAA+TGG - chr5_4:30095856-30095875 Msa0847640:three_prime_UTR 15.0%
!! TATAAGAATTGTTATATTCA+AGG - chr5_4:30096859-30096878 Msa0847640:intron 15.0%
!!! AACTTGCTTAATTTATAATT+TGG - chr5_4:30097553-30097572 Msa0847640:three_prime_UTR 15.0%
!! ACTTGCTTAATTTATAATTT+GGG - chr5_4:30097554-30097573 Msa0847640:three_prime_UTR 15.0%
!! AATGTATAATATCATTTGTT+GGG + chr5_4:30098473-30098492 None:intergenic 15.0%
!!! TTTACATTTTAGCAAATTAA+AGG + chr5_4:30099469-30099488 None:intergenic 15.0%
!!! AATTTGCTAAAATGTAAAAA+AGG - chr5_4:30099471-30099490 Msa0847640:intron 15.0%
!!! TTTTAATTTGGTTTGTTGTT+TGG - chr5_4:30094457-30094476 Msa0847640:three_prime_UTR 20.0%
!! AAATAGCACTCATTTAATAA+CGG + chr5_4:30095404-30095423 None:intergenic 20.0%
!!! ATGATGGATTTTGTTAATAT+TGG - chr5_4:30095516-30095535 Msa0847640:three_prime_UTR 20.0%
!!! TGTTAATATTGGTGTTTTTT+TGG - chr5_4:30095527-30095546 Msa0847640:three_prime_UTR 20.0%
!! GATATAAGTTCTTACATAAT+AGG + chr5_4:30095824-30095843 None:intergenic 20.0%
!! ATTATGTAAGAACTTATATC+AGG - chr5_4:30095824-30095843 Msa0847640:three_prime_UTR 20.0%
!!! ATGATTATATTAGTTTCTTG+TGG - chr5_4:30096606-30096625 Msa0847640:intron 20.0%
!!! TATCTTTGATTGTAGTTTTT+TGG - chr5_4:30096795-30096814 Msa0847640:intron 20.0%
!!! ATCTTTGATTGTAGTTTTTT+GGG - chr5_4:30096796-30096815 Msa0847640:intron 20.0%
!! TATTACCTCAAAATAAGATT+GGG + chr5_4:30096911-30096930 None:intergenic 20.0%
!! TTATTACCTCAAAATAAGAT+TGG + chr5_4:30096912-30096931 None:intergenic 20.0%
!! AGTACTTAGGAAATTATTAT+TGG - chr5_4:30097136-30097155 Msa0847640:intron 20.0%
!! TAATTTGATTAGAAAGTTGA+AGG - chr5_4:30097498-30097517 Msa0847640:intron 20.0%
!! CTTGCTTAATTTATAATTTG+GGG - chr5_4:30097555-30097574 Msa0847640:three_prime_UTR 20.0%
!!! GTCATTTTAGCATAATTTTT+TGG - chr5_4:30097695-30097714 Msa0847640:three_prime_UTR 20.0%
!! CAATGTATAATATCATTTGT+TGG + chr5_4:30098474-30098493 None:intergenic 20.0%
!! TTGAAAATTATGTAAAAAGC+AGG - chr5_4:30098511-30098530 Msa0847640:intron 20.0%
!! TGAAAATTATGTAAAAAGCA+GGG - chr5_4:30098512-30098531 Msa0847640:intron 20.0%
!! TAACTGAAAGAAAGAAAAAA+AGG + chr5_4:30098812-30098831 None:intergenic 20.0%
!!! TGAAATGAAATTGAGTTTTT+AGG - chr5_4:30099067-30099086 Msa0847640:intron 20.0%
!!! ATTTTTGTATCGCAATTAAA+AGG - chr5_4:30099395-30099414 Msa0847640:CDS 20.0%
!! ATGAAAAATGAGCAAATTAA+GGG + chr5_4:30099526-30099545 None:intergenic 20.0%
!!! GTACTAAAATTTCCAATTTT+TGG - chr5_4:30099745-30099764 Msa0847640:intron 20.0%
!! AAAAAATTGCTTAATAAGAG+AGG + chr5_4:30100305-30100324 None:intergenic 20.0%
!!! ATTTCGTTTTTGATCAATTG+AGG - chr5_4:30094203-30094222 Msa0847640:three_prime_UTR 25.0%
!!! TTCTGGGTTTGATTTTAATT+TGG - chr5_4:30094445-30094464 Msa0847640:three_prime_UTR 25.0%
!!! TTTCAGTTTTTTGTTTGGTT+TGG - chr5_4:30094483-30094502 Msa0847640:three_prime_UTR 25.0%
! AAGTGTAAGATTATGTCATT+AGG - chr5_4:30094596-30094615 Msa0847640:three_prime_UTR 25.0%
! TAAAATAAGCTAATCCAAAC+AGG - chr5_4:30094812-30094831 Msa0847640:intron 25.0%
!! TAGTGTAATTACTGTTTCTT+CGG - chr5_4:30095001-30095020 Msa0847640:intron 25.0%
! ACAAAGAGATAATTAAGAGT+GGG - chr5_4:30095186-30095205 Msa0847640:three_prime_UTR 25.0%
! TTTCATGTATTCATTCATGA+TGG - chr5_4:30095500-30095519 Msa0847640:three_prime_UTR 25.0%
! ATATTGTTCATATGCTAAGT+AGG - chr5_4:30095599-30095618 Msa0847640:three_prime_UTR 25.0%
!! TTCATGATATAGAAGCTTTT+TGG - chr5_4:30095895-30095914 Msa0847640:intron 25.0%
!!! TTTTGATGTTTTGATTGTGT+GGG - chr5_4:30096003-30096022 Msa0847640:intron 25.0%
! TCTTGCATATTATACAGTAT+AGG - chr5_4:30096058-30096077 Msa0847640:three_prime_UTR 25.0%
!! AGAAAAGCATATATGAGATT+AGG + chr5_4:30096207-30096226 None:intergenic 25.0%
! AGGAGATAAAAGTTATGTTT+AGG - chr5_4:30096300-30096319 Msa0847640:intron 25.0%
! TAAACAACAAGAAATTGAGA+AGG - chr5_4:30096396-30096415 Msa0847640:intron 25.0%
! ATTAAAATCATTGCTTAGTG+AGG + chr5_4:30096680-30096699 None:intergenic 25.0%
!!! AAACAATGAAGCATTTTAAG+TGG - chr5_4:30096974-30096993 Msa0847640:intron 25.0%
! ACTTACATTCAAATTTATGC+AGG - chr5_4:30097179-30097198 Msa0847640:intron 25.0%
! CTTACATTCAAATTTATGCA+GGG - chr5_4:30097180-30097199 Msa0847640:intron 25.0%
! AAATTTATGCAGGGAATAAT+AGG - chr5_4:30097189-30097208 Msa0847640:intron 25.0%
!! AAGCATTAGTAAAAGTTGAT+AGG - chr5_4:30097325-30097344 Msa0847640:intron 25.0%
! TGTATGAAGTTTGATCATAA+TGG - chr5_4:30097764-30097783 Msa0847640:intron 25.0%
!!! TTTGATCATAATGGTTTGTT+GGG - chr5_4:30097773-30097792 Msa0847640:intron 25.0%
!! TTAATCATTTTGAACAAGAG+AGG - chr5_4:30097827-30097846 Msa0847640:intron 25.0%
! TTCATAAAACTTGCATTTCA+TGG - chr5_4:30097943-30097962 Msa0847640:intron 25.0%
! CATAGATCTATATAAGATCT+TGG + chr5_4:30098000-30098019 None:intergenic 25.0%
! GATCTTATATAGATCTATGT+TGG - chr5_4:30098001-30098020 Msa0847640:intron 25.0%
! GCAAATATTGCAAAAAGAAA+AGG + chr5_4:30098253-30098272 None:intergenic 25.0%
! TTAAATAATTCTGATCACAG+AGG + chr5_4:30098312-30098331 None:intergenic 25.0%
! CTGTGATCAGAATTATTTAA+TGG - chr5_4:30098312-30098331 Msa0847640:intron 25.0%
! TTATCTCTTTATTCTGTACA+GGG - chr5_4:30098575-30098594 Msa0847640:intron 25.0%
! ATAAAGCATACCAAAATCAA+GGG + chr5_4:30098987-30099006 None:intergenic 25.0%
! CAGCATTATAAAAGACAAAA+TGG + chr5_4:30099018-30099037 None:intergenic 25.0%
! CAACAATGATACTAAACTAT+TGG - chr5_4:30099275-30099294 Msa0847640:CDS 25.0%
! CATGAAAAATGAGCAAATTA+AGG + chr5_4:30099527-30099546 None:intergenic 25.0%
!! TCTATAGGATTTGAGATTTA+AGG - chr5_4:30099646-30099665 Msa0847640:intron 25.0%
!! TGAGTGATTGAAATTTTGTT+TGG - chr5_4:30099717-30099736 Msa0847640:intron 25.0%
!!! ATTGAAATTTTGTTTGGCTT+TGG - chr5_4:30099723-30099742 Msa0847640:intron 25.0%
! AATCTGCAATAACCAAAAAT+TGG + chr5_4:30099760-30099779 None:intergenic 25.0%
!! TAAAGTTTTCCTTTCATGAA+AGG + chr5_4:30099827-30099846 None:intergenic 25.0%
! CCATATAGGATATCTTTATA+CGG + chr5_4:30100222-30100241 None:intergenic 25.0%
! TCATCTTGTTACAATTTCAT+AGG + chr5_4:30100351-30100370 None:intergenic 25.0%
TGAAGAAGAAGATGAAAAAC+AGG + chr5_4:30094109-30094128 None:intergenic 30.0%
! TGTTTTTCATCTTCTTCTTC+AGG - chr5_4:30094108-30094127 Msa0847640:three_prime_UTR 30.0%
!! TCTGTCAATTTTGATCGATT+GGG - chr5_4:30094162-30094181 Msa0847640:three_prime_UTR 30.0%
! TTTTCCTTCATGTTTTCAAC+AGG - chr5_4:30094238-30094257 Msa0847640:three_prime_UTR 30.0%
AGATTGAATTGTGAAGAGTA+TGG - chr5_4:30094281-30094300 Msa0847640:three_prime_UTR 30.0%
!!! TATGGTGAGTTTTGTTTGAT+TGG - chr5_4:30094299-30094318 Msa0847640:three_prime_UTR 30.0%
!!! ATGGTGAGTTTTGTTTGATT+GGG - chr5_4:30094300-30094319 Msa0847640:three_prime_UTR 30.0%
GTTGCAAAATTGAAGTTTCT+AGG - chr5_4:30094376-30094395 Msa0847640:three_prime_UTR 30.0%
!! AAGTTTCTAGGTTTGTTGTT+TGG - chr5_4:30094388-30094407 Msa0847640:three_prime_UTR 30.0%
!!! GGTGATTTCAGTTTTTTGTT+TGG - chr5_4:30094478-30094497 Msa0847640:three_prime_UTR 30.0%
! GCACAAATTTTATCCTTGAT+CGG - chr5_4:30094515-30094534 Msa0847640:three_prime_UTR 30.0%
AAAATAAGCTCAAACCAAAC+GGG + chr5_4:30094623-30094642 None:intergenic 30.0%
CAAAATAAGCTCAAACCAAA+CGG + chr5_4:30094624-30094643 None:intergenic 30.0%
! ATGAAGATACAATTTTCGTC+AGG + chr5_4:30094706-30094725 None:intergenic 30.0%
AAGGTAGTTTATGAACAAAC+AGG + chr5_4:30094731-30094750 None:intergenic 30.0%
! TAAATTATTTAGGGCCTGTT+TGG + chr5_4:30094829-30094848 None:intergenic 30.0%
!!! TTGCCGGTTTTTTTTAGTTA+TGG - chr5_4:30095157-30095176 Msa0847640:three_prime_UTR 30.0%
GCTCCATAACTAAAAAAAAC+CGG + chr5_4:30095163-30095182 None:intergenic 30.0%
CACAAAGAGATAATTAAGAG+TGG - chr5_4:30095185-30095204 Msa0847640:three_prime_UTR 30.0%
! TACACAATTTTCGTGCTTAT+TGG - chr5_4:30095373-30095392 Msa0847640:intron 30.0%
!! GAGTGCTATTTACATTCTAT+AGG - chr5_4:30095413-30095432 Msa0847640:intron 30.0%
TCTATCTTTGAACTAACTTG+AGG - chr5_4:30095550-30095569 Msa0847640:three_prime_UTR 30.0%
AACTTATATCAGGAATGAAG+TGG - chr5_4:30095834-30095853 Msa0847640:three_prime_UTR 30.0%
!!! GTTTTGATGTTTTGATTGTG+TGG - chr5_4:30096002-30096021 Msa0847640:intron 30.0%
! ACTTAGCATATTTTCTGCTT+TGG - chr5_4:30096032-30096051 Msa0847640:three_prime_UTR 30.0%
AAATGTATCGAGTTTCTGAA+TGG - chr5_4:30096150-30096169 Msa0847640:intron 30.0%
ATATATGAGATTAGGACTTC+AGG + chr5_4:30096199-30096218 None:intergenic 30.0%
! AACATAACTTTTATCTCCTC+TGG + chr5_4:30096299-30096318 None:intergenic 30.0%
AAGGCAAAACAACAATTAGA+TGG - chr5_4:30096415-30096434 Msa0847640:intron 30.0%
AAAACAACAATTAGATGGCA+AGG - chr5_4:30096420-30096439 Msa0847640:intron 30.0%
TCTACTAATTAGACTGTTGA+AGG + chr5_4:30096640-30096659 None:intergenic 30.0%
! AAGTGGTTTTTACCTATTGT+AGG - chr5_4:30096991-30097010 Msa0847640:intron 30.0%
CAAACACAAATGTAGAAAAC+AGG + chr5_4:30097115-30097134 None:intergenic 30.0%
! CATTTGTGTTTGAAGTACTT+AGG - chr5_4:30097123-30097142 Msa0847640:intron 30.0%
ACTTTCAAATGCTCTAATCA+CGG + chr5_4:30097259-30097278 None:intergenic 30.0%
AAAGAATCTGTATGAAGAGA+TGG + chr5_4:30097285-30097304 None:intergenic 30.0%
!! ATTAGTAAAAGTTGATAGGC+TGG - chr5_4:30097329-30097348 Msa0847640:intron 30.0%
!! CTTTTCTAAGAGTGACTATT+TGG - chr5_4:30097373-30097392 Msa0847640:intron 30.0%
TTGACCATTATTGTTTGACT+AGG - chr5_4:30097448-30097467 Msa0847640:intron 30.0%
GTTTCCTAGTCAAACAATAA+TGG + chr5_4:30097455-30097474 None:intergenic 30.0%
! GTTTGATCATAATGGTTTGT+TGG - chr5_4:30097772-30097791 Msa0847640:intron 30.0%
!! TTGATCATAATGGTTTGTTG+GGG - chr5_4:30097774-30097793 Msa0847640:intron 30.0%
!! CTCTTTTGACCTTTTGTTTA+GGG - chr5_4:30097903-30097922 Msa0847640:intron 30.0%
TAAAACTTGCATTTCATGGA+TGG - chr5_4:30097947-30097966 Msa0847640:intron 30.0%
AAAACTTGCATTTCATGGAT+GGG - chr5_4:30097948-30097967 Msa0847640:intron 30.0%
TCATGGATGGGATAAATAAA+TGG - chr5_4:30097960-30097979 Msa0847640:intron 30.0%
! TCTTATCGCTGATTATAGTT+AGG - chr5_4:30098141-30098160 Msa0847640:three_prime_UTR 30.0%
TGATTATAGTTAGGCTTTCT+GGG - chr5_4:30098150-30098169 Msa0847640:three_prime_UTR 30.0%
TATAGTGATTGTAGATGATC+TGG - chr5_4:30098187-30098206 Msa0847640:exon 30.0%
ATAGTGATTGTAGATGATCT+GGG - chr5_4:30098188-30098207 Msa0847640:CDS 30.0%
AAAGAAAAGGAGATTGATGA+AGG + chr5_4:30098240-30098259 None:intergenic 30.0%
TTGCAATATTTGCAACTACT+CGG - chr5_4:30098260-30098279 Msa0847640:intron 30.0%
ACCTAAACAAAGAATCACAA+AGG + chr5_4:30098286-30098305 None:intergenic 30.0%
! TTGTGATTCTTTGTTTAGGT+CGG - chr5_4:30098286-30098305 Msa0847640:intron 30.0%
!!! TCGTTCTTCTTTTTTGTTCT+AGG - chr5_4:30098371-30098390 Msa0847640:intron 30.0%
! TTATGTAAAAAGCAGGGTTT+TGG - chr5_4:30098518-30098537 Msa0847640:intron 30.0%
! TATGTAAAAAGCAGGGTTTT+GGG - chr5_4:30098519-30098538 Msa0847640:intron 30.0%
AAGAGATAAGCTAAGAATGA+AGG + chr5_4:30098564-30098583 None:intergenic 30.0%
CTTATCTCTTTATTCTGTAC+AGG - chr5_4:30098574-30098593 Msa0847640:intron 30.0%
AGAAAGAAAAAAAGGTGAGA+GGG + chr5_4:30098804-30098823 None:intergenic 30.0%
AAGAAAGAAAAAAAGGTGAG+AGG + chr5_4:30098805-30098824 None:intergenic 30.0%
GATAAAGCATACCAAAATCA+AGG + chr5_4:30098988-30099007 None:intergenic 30.0%
TGTCTAACAATACCAGTAAA+TGG + chr5_4:30099171-30099190 None:intergenic 30.0%
CAATGATACTAAACTATTGG+TGG - chr5_4:30099278-30099297 Msa0847640:CDS 30.0%
AATGATTTACCATTGAAACC+AGG - chr5_4:30099324-30099343 Msa0847640:intron 30.0%
!! AGAATAAAAGTATTGCACAC+AGG + chr5_4:30099439-30099458 None:intergenic 30.0%
ACTTTACATTAATTTCGCTG+TGG - chr5_4:30099841-30099860 Msa0847640:intron 30.0%
CCGTATAAAGATATCCTATA+TGG - chr5_4:30100219-30100238 Msa0847640:intron 30.0%
!! CTCTGTCAATTTTGATCGAT+TGG - chr5_4:30094161-30094180 Msa0847640:three_prime_UTR 35.0%
AAATAAGCTCAAACCAAACG+GGG + chr5_4:30094622-30094641 None:intergenic 35.0%
TGTAATGTGACTACTGTGTT+TGG - chr5_4:30094873-30094892 Msa0847640:intron 35.0%
! ATTACTGTTTCTTCGGACAA+AGG - chr5_4:30095008-30095027 Msa0847640:intron 35.0%
TCTTTGTGTGTGTATTGTGA+TGG - chr5_4:30095107-30095126 Msa0847640:intron 35.0%
GTGTTGAATGTTACATTTGC+CGG - chr5_4:30095141-30095160 Msa0847640:three_prime_UTR 35.0%
TATTTCTCCCATCGTCATTA+AGG + chr5_4:30095279-30095298 None:intergenic 35.0%
TTTCTCAATGCTGTATTGCA+AGG + chr5_4:30095463-30095482 None:intergenic 35.0%
! GCAATACAGCATTGAGAAAA+TGG - chr5_4:30095464-30095483 Msa0847640:intron 35.0%
TCAATACTAAATGCATCCTC+TGG + chr5_4:30095648-30095667 None:intergenic 35.0%
AGAGGATGCATTTAGTATTG+AGG - chr5_4:30095647-30095666 Msa0847640:three_prime_UTR 35.0%
AGGATGAAAGAAATTGCTCA+TGG - chr5_4:30095667-30095686 Msa0847640:three_prime_UTR 35.0%
GGATGAAAGAAATTGCTCAT+GGG - chr5_4:30095668-30095687 Msa0847640:three_prime_UTR 35.0%
GTATTAATCCCTTACTTGGT+CGG + chr5_4:30095743-30095762 None:intergenic 35.0%
AGCAGTATTAATCCCTTACT+TGG + chr5_4:30095747-30095766 None:intergenic 35.0%
! AAGCTTTTTGGATCATAGCA+AGG - chr5_4:30095907-30095926 Msa0847640:intron 35.0%
! CCAATTTCAATAGGTTGAGT+TGG + chr5_4:30096120-30096139 None:intergenic 35.0%
CCAACTCAACCTATTGAAAT+TGG - chr5_4:30096117-30096136 Msa0847640:intron 35.0%
AATGATCTTGCCTAACTTAC+CGG - chr5_4:30096444-30096463 Msa0847640:intron 35.0%
GTGACTTAATTCAGGGATTA+GGG + chr5_4:30096493-30096512 None:intergenic 35.0%
TGTGACTTAATTCAGGGATT+AGG + chr5_4:30096494-30096513 None:intergenic 35.0%
ACGACATGTGACTTAATTCA+GGG + chr5_4:30096500-30096519 None:intergenic 35.0%
TCGTGTGATATTTGTATGTG+AGG - chr5_4:30096516-30096535 Msa0847640:intron 35.0%
TGTATGTGAGGTTACAATTG+AGG - chr5_4:30096528-30096547 Msa0847640:intron 35.0%
CAGAACACACATGGAAATAA+TGG - chr5_4:30096733-30096752 Msa0847640:intron 35.0%
! CACACATGGAAATAATGGAA+AGG - chr5_4:30096738-30096757 Msa0847640:intron 35.0%
! GGTGAAGTTGATTTCAACTT+TGG - chr5_4:30096880-30096899 Msa0847640:intron 35.0%
!!! TCAAGCCCAATCTTATTTTG+AGG - chr5_4:30096903-30096922 Msa0847640:intron 35.0%
ATGTTGCACAATCCTACAAT+AGG + chr5_4:30097006-30097025 None:intergenic 35.0%
CAAGAGAAAATGAGCAAATC+TGG + chr5_4:30097399-30097418 None:intergenic 35.0%
CCTAAACAAAAGGTCAAAAG+AGG + chr5_4:30097905-30097924 None:intergenic 35.0%
!! CCTCTTTTGACCTTTTGTTT+AGG - chr5_4:30097902-30097921 Msa0847640:intron 35.0%
!! TTTGACCTTTTGTTTAGGGA+TGG - chr5_4:30097907-30097926 Msa0847640:intron 35.0%
!! TTGACCTTTTGTTTAGGGAT+GGG - chr5_4:30097908-30097927 Msa0847640:intron 35.0%
TGGATGGGATAAATAAATGG+TGG - chr5_4:30097963-30097982 Msa0847640:intron 35.0%
AGTCTGCAGAATTCAAAACT+TGG - chr5_4:30098024-30098043 Msa0847640:intron 35.0%
CTGATTATAGTTAGGCTTTC+TGG - chr5_4:30098149-30098168 Msa0847640:three_prime_UTR 35.0%
! GCCTTTGTGATTCTTTGTTT+AGG - chr5_4:30098282-30098301 Msa0847640:intron 35.0%
!! TTTGTTCTAGGAACGTGTTA+AGG - chr5_4:30098383-30098402 Msa0847640:intron 35.0%
! ATTTGTTGGGTTTACCTCAA+AGG + chr5_4:30098460-30098479 None:intergenic 35.0%
! TATTCTGTACAGGGTTTTCA+AGG - chr5_4:30098584-30098603 Msa0847640:intron 35.0%
AGAGAAGACTAATATCGTGA+TGG + chr5_4:30098733-30098752 None:intergenic 35.0%
AGAAACTAACAAATCTGCGA+AGG + chr5_4:30098772-30098791 None:intergenic 35.0%
GAAAGAAAAAAAGGTGAGAG+GGG + chr5_4:30098803-30098822 None:intergenic 35.0%
ATTTACTCTCTTCTTCGCTA+TGG - chr5_4:30098851-30098870 Msa0847640:CDS 35.0%
TTAATCTATTTGATGAGCCG+AGG - chr5_4:30098882-30098901 Msa0847640:intron 35.0%
!!! ATGCTGTGCTGTTTCATTTT+TGG - chr5_4:30099032-30099051 Msa0847640:intron 35.0%
AAATACGTGAGCAAGAAAGA+AGG - chr5_4:30099093-30099112 Msa0847640:intron 35.0%
! TGTGGAAGCATAATGTCAAT+GGG - chr5_4:30099119-30099138 Msa0847640:intron 35.0%
ATAGCTTACCAAATGCAAAC+AGG + chr5_4:30099225-30099244 None:intergenic 35.0%
! AACCCAACTTGATAATACCA+AGG - chr5_4:30099608-30099627 Msa0847640:intron 35.0%
! ACCCAACTTGATAATACCAA+GGG - chr5_4:30099609-30099628 Msa0847640:intron 35.0%
!!! GTTTTTTTCTCTCTGACATC+TGG - chr5_4:30099670-30099689 Msa0847640:intron 35.0%
GGTTATTGCAGATTCTTACA+AGG - chr5_4:30099766-30099785 Msa0847640:intron 35.0%
TACAAGGCAATCAATGATGA+AGG - chr5_4:30099782-30099801 Msa0847640:intron 35.0%
TAACTTGATCAAGAGACTCA+GGG + chr5_4:30099871-30099890 None:intergenic 35.0%
! TTAACTTGATCAAGAGACTC+AGG + chr5_4:30099872-30099891 None:intergenic 35.0%
TGAGTCTCTTGATCAAGTTA+AGG - chr5_4:30099871-30099890 Msa0847640:intron 35.0%
TCTTGATCAAGTTAAGGCTT+TGG - chr5_4:30099877-30099896 Msa0847640:intron 35.0%
TTGAAGAAGAGACAAGAAGA+AGG - chr5_4:30099987-30100006 Msa0847640:intron 35.0%
AAGCTAAAACACATGCATGA+AGG + chr5_4:30100134-30100153 None:intergenic 35.0%
!!! TGCATGTGTTTTAGCTTTGT+TGG - chr5_4:30100137-30100156 Msa0847640:intron 35.0%
!!! GCATGTGTTTTAGCTTTGTT+GGG - chr5_4:30100138-30100157 Msa0847640:intron 35.0%
! CTGCAATTTTGCAAACACAA+GGG + chr5_4:30100263-30100282 None:intergenic 35.0%
ATAGGAAAATGAAACACGCA+AGG + chr5_4:30100333-30100352 None:intergenic 35.0%
!! GTCAATTTTGATCGATTGGG+AGG - chr5_4:30094165-30094184 Msa0847640:three_prime_UTR 40.0%
GACACCTGTTGAAAACATGA+AGG + chr5_4:30094245-30094264 None:intergenic 40.0%
! ATGTTTTCAACAGGTGTCTG+AGG - chr5_4:30094247-30094266 Msa0847640:three_prime_UTR 40.0%
!! TCTTCGGACAAAGGTATTTG+TGG - chr5_4:30095017-30095036 Msa0847640:intron 40.0%
! ACCAGAATTCAACTTGCTCA+AGG + chr5_4:30095078-30095097 None:intergenic 40.0%
!! GAGTGGGTGTCATAAGATTT+TGG - chr5_4:30095202-30095221 Msa0847640:intron 40.0%
!! GGGTGTCATAAGATTTTGGA+AGG - chr5_4:30095206-30095225 Msa0847640:intron 40.0%
TCATTAAGGAGTGAACTTGC+TGG + chr5_4:30095265-30095284 None:intergenic 40.0%
AGTTCACTCCTTAATGACGA+TGG - chr5_4:30095268-30095287 Msa0847640:intron 40.0%
GTTCACTCCTTAATGACGAT+GGG - chr5_4:30095269-30095288 Msa0847640:intron 40.0%
TGTTGTTGGTCGAGATAGAA+AGG - chr5_4:30095310-30095329 Msa0847640:three_prime_UTR 40.0%
! ACTAACTTGAGGTAGTTTGC+AGG - chr5_4:30095561-30095580 Msa0847640:three_prime_UTR 40.0%
CTAAGTAGGATGTCCTATGA+AGG - chr5_4:30095613-30095632 Msa0847640:three_prime_UTR 40.0%
TTGGATCATAGCAAGGTTTG+TGG - chr5_4:30095914-30095933 Msa0847640:intron 40.0%
!!! GATGTTTTGATTGTGTGGGT+TGG - chr5_4:30096007-30096026 Msa0847640:intron 40.0%
AGCTGCAGACCAATTTCAAT+AGG + chr5_4:30096129-30096148 None:intergenic 40.0%
! ACTTTCTGAGAACATACCAG+AGG - chr5_4:30096280-30096299 Msa0847640:intron 40.0%
ATGATCTTGCCTAACTTACC+GGG - chr5_4:30096445-30096464 Msa0847640:intron 40.0%
GATATGGATGTTTGGATTCC+CGG + chr5_4:30096466-30096485 None:intergenic 40.0%
AGGGTCAAGATATGGATGTT+TGG + chr5_4:30096474-30096493 None:intergenic 40.0%
CACGACATGTGACTTAATTC+AGG + chr5_4:30096501-30096520 None:intergenic 40.0%
GTGTAATTGCAGAACACACA+TGG - chr5_4:30096724-30096743 Msa0847640:intron 40.0%
GCAGTAGCTGCTACTATAAT+CGG - chr5_4:30096760-30096779 Msa0847640:intron 40.0%
TGGGTTATCAAGACACTTTG+TGG - chr5_4:30096815-30096834 Msa0847640:intron 40.0%
GGGTTATCAAGACACTTTGT+GGG - chr5_4:30096816-30096835 Msa0847640:intron 40.0%
TCAGGAAGAATACTGATGAG+AGG + chr5_4:30097049-30097068 None:intergenic 40.0%
! AAAACCTCAGTTTTGCAGTC+AGG + chr5_4:30097067-30097086 None:intergenic 40.0%
AGGATCATAAGAGGCTTTCT+CGG + chr5_4:30097232-30097251 None:intergenic 40.0%
AATGCTCTAATCACGGATTC+AGG + chr5_4:30097252-30097271 None:intergenic 40.0%
TGTTCCCATCCCTAAACAAA+AGG + chr5_4:30097915-30097934 None:intergenic 40.0%
! GATATGGTGCAATTGAGTTG+AGG - chr5_4:30098088-30098107 Msa0847640:three_prime_UTR 40.0%
ACGTAAGTATGATCAGTGAC+TGG - chr5_4:30098346-30098365 Msa0847640:intron 40.0%
! TTTTGAGCTTGATGTTGGCA+TGG + chr5_4:30098663-30098682 None:intergenic 40.0%
!!! CTTGGTTTTGAGCTTGATGT+TGG + chr5_4:30098668-30098687 None:intergenic 40.0%
ATGCGAAGATTTGACAACCT+CGG + chr5_4:30098902-30098921 None:intergenic 40.0%
CATACCAAAATCAAGGGCTT+CGG + chr5_4:30098981-30099000 None:intergenic 40.0%
! CTGTGGAAGCATAATGTCAA+TGG - chr5_4:30099118-30099137 Msa0847640:intron 40.0%
GAAGCATAATGTCAATGGGT+TGG - chr5_4:30099123-30099142 Msa0847640:intron 40.0%
TTCTGTCTCCTGTTTGCATT+TGG - chr5_4:30099214-30099233 Msa0847640:CDS 40.0%
AAAACTATATCTGTCGTGCC+TGG + chr5_4:30099345-30099364 None:intergenic 40.0%
! GCACGACAGATATAGTTTTC+AGG - chr5_4:30099346-30099365 Msa0847640:intron 40.0%
GCCCTTGGTATTATCAAGTT+GGG + chr5_4:30099613-30099632 None:intergenic 40.0%
GCGACATCAAATGCTTCTAT+AGG - chr5_4:30099631-30099650 Msa0847640:intron 40.0%
ATGAAAGGTCTCTGGTACAT+TGG + chr5_4:30099812-30099831 None:intergenic 40.0%
TACCAGAGACCTTTCATGAA+AGG - chr5_4:30099815-30099834 Msa0847640:intron 40.0%
TTCCTTTCATGAAAGGTCTC+TGG + chr5_4:30099820-30099839 None:intergenic 40.0%
TGTATCACAGTTGACAGACA+TGG - chr5_4:30099922-30099941 Msa0847640:intron 40.0%
GTGCTAAGCAGTAATGAGTT+TGG - chr5_4:30100077-30100096 Msa0847640:intron 40.0%
CTTTGTTGGGCTAAACATAG+TGG - chr5_4:30100151-30100170 Msa0847640:intron 40.0%
! GCTGCAATTTTGCAAACACA+AGG + chr5_4:30100264-30100283 None:intergenic 40.0%
GAAAATGAAACACGCAAGGA+AGG + chr5_4:30100329-30100348 None:intergenic 40.0%
! TTTTGATCGATTGGGAGGAC+TGG - chr5_4:30094170-30094189 Msa0847640:three_prime_UTR 45.0%
TTTGATCGATTGGGAGGACT+GGG - chr5_4:30094171-30094190 Msa0847640:three_prime_UTR 45.0%
TTATGTCATTAGGCCCCGTT+TGG - chr5_4:30094606-30094625 Msa0847640:three_prime_UTR 45.0%
!! TCGGACAAAGGTATTTGTGG+TGG - chr5_4:30095020-30095039 Msa0847640:intron 45.0%
CACGTCTGTCAAGATAAGCA+GGG - chr5_4:30095052-30095071 Msa0847640:intron 45.0%
! GCCTTGAGCAAGTTGAATTC+TGG - chr5_4:30095074-30095093 Msa0847640:intron 45.0%
AAGCAACCTAGAGTTGCAAC+AGG - chr5_4:30095700-30095719 Msa0847640:three_prime_UTR 45.0%
AACCTAGAGTTGCAACAGGT+AGG - chr5_4:30095704-30095723 Msa0847640:three_prime_UTR 45.0%
AGTTACTTGCCGACCAAGTA+AGG - chr5_4:30095731-30095750 Msa0847640:three_prime_UTR 45.0%
GTTACTTGCCGACCAAGTAA+GGG - chr5_4:30095732-30095751 Msa0847640:three_prime_UTR 45.0%
GTTTGGATTCCCGGTAAGTT+AGG + chr5_4:30096457-30096476 None:intergenic 45.0%
CAGGGATTAGGGTCAAGATA+TGG + chr5_4:30096482-30096501 None:intergenic 45.0%
AGACACTTTGTGGGATGCAT+TGG - chr5_4:30096825-30096844 Msa0847640:intron 45.0%
GACACTTTGTGGGATGCATT+GGG - chr5_4:30096826-30096845 Msa0847640:intron 45.0%
GGAAGAATACTGATGAGAGG+TGG + chr5_4:30097046-30097065 None:intergenic 45.0%
TCTTCCTGACTGCAAAACTG+AGG - chr5_4:30097060-30097079 Msa0847640:intron 45.0%
CACGGATTCAGGATCATAAG+AGG + chr5_4:30097241-30097260 None:intergenic 45.0%
AGTTGAGGAGATCAATGCGA+AGG - chr5_4:30098103-30098122 Msa0847640:three_prime_UTR 45.0%
CAATGCGAAGGTTGTGTCAT+AGG - chr5_4:30098115-30098134 Msa0847640:three_prime_UTR 45.0%
GAATCTTCACAGTTCACTCC+TGG - chr5_4:30098426-30098445 Msa0847640:intron 45.0%
!!! TAAAAAGCAGGGTTTTGGGC+AGG - chr5_4:30098523-30098542 Msa0847640:intron 45.0%
TAGTAACTTCACGACAGAGG+TGG + chr5_4:30098943-30098962 None:intergenic 45.0%
CCTTAGTAACTTCACGACAG+AGG + chr5_4:30098946-30098965 None:intergenic 45.0%
CCTCTGTCGTGAAGTTACTA+AGG - chr5_4:30098943-30098962 Msa0847640:intron 45.0%
TGCATCTGCACACCATTTAC+TGG - chr5_4:30099156-30099175 Msa0847640:CDS 45.0%
CGCCCTTGGTATTATCAAGT+TGG + chr5_4:30099614-30099633 None:intergenic 45.0%
AACTGTGATACAGACCTTGC+TGG + chr5_4:30099914-30099933 None:intergenic 45.0%
GATGAAGATGATGAGATGCC+TGG - chr5_4:30100034-30100053 Msa0847640:intron 45.0%
ATGAGTTTGGAGATGTGCTG+TGG - chr5_4:30100090-30100109 Msa0847640:intron 45.0%
ATGTGCTGTGGTAGAACCTT+TGG - chr5_4:30100102-30100121 Msa0847640:intron 45.0%
TGCATGAAGGACATCACCAA+AGG + chr5_4:30100121-30100140 None:intergenic 45.0%
AGAGCTGCAGAAGCGAATTC+CGG + chr5_4:30094562-30094581 None:intergenic 50.0%
CCTGCTTATCTTGACAGACG+TGG + chr5_4:30095054-30095073 None:intergenic 50.0%
CCACGTCTGTCAAGATAAGC+AGG - chr5_4:30095051-30095070 Msa0847640:intron 50.0%
ATAGCGCGTGGAGTTGTTGT+TGG - chr5_4:30095296-30095315 Msa0847640:intron 50.0%
CTGGTCATGCAAGCCTTCAT+AGG + chr5_4:30095629-30095648 None:intergenic 50.0%
ATGAAGGCTTGCATGACCAG+AGG - chr5_4:30095629-30095648 Msa0847640:three_prime_UTR 50.0%
ACTTGGTCGGCAAGTAACTC+AGG + chr5_4:30095730-30095749 None:intergenic 50.0%
TACTGATGAGAGGTGGAGTG+TGG + chr5_4:30097039-30097058 None:intergenic 50.0%
GATGAAGGTCGAGAGGACAT+AGG + chr5_4:30098225-30098244 None:intergenic 50.0%
GGAGATTGATGAAGGTCGAG+AGG + chr5_4:30098232-30098251 None:intergenic 50.0%
TCCTGGTGCTTAGTCCTTTG+AGG - chr5_4:30098443-30098462 Msa0847640:intron 50.0%
ACCTCAAAGGACTAAGCACC+AGG + chr5_4:30098447-30098466 None:intergenic 50.0%
! TTTCAAGGCAGAAGTGCAGC+TGG - chr5_4:30098599-30098618 Msa0847640:intron 50.0%
GCTGATAGTCCGGTTTCCTT+CGG - chr5_4:30098623-30098642 Msa0847640:intron 50.0%
TCATCATCGCCGAAGGAAAC+CGG + chr5_4:30098635-30098654 None:intergenic 50.0%
AGCTCAAAACCAAGCACCAG+AGG - chr5_4:30098674-30098693 Msa0847640:intron 50.0%
CAAAACCAAGCACCAGAGGT+CGG - chr5_4:30098678-30098697 Msa0847640:intron 50.0%
AACAGATCCTTTCCGACCTC+TGG + chr5_4:30098693-30098712 None:intergenic 50.0%
! CATGCCGAAGCCCTTGATTT+TGG - chr5_4:30098974-30098993 Msa0847640:intron 50.0%
GAGCAAGAAAGAAGGCTCTG+TGG - chr5_4:30099101-30099120 Msa0847640:intron 50.0%
TGGTGTGCAGATGCAACACT+CGG + chr5_4:30099151-30099170 None:intergenic 50.0%
TCTGTCGTGCCTGGTTTCAA+TGG + chr5_4:30099336-30099355 None:intergenic 50.0%
! GAAGCATTTGATGTCGCCCT+TGG + chr5_4:30099628-30099647 None:intergenic 50.0%
TGGAGACAATTCTGCCAGCA+AGG - chr5_4:30099897-30099916 Msa0847640:intron 50.0%
ACAGTTGACAGACATGGAGC+TGG - chr5_4:30099928-30099947 Msa0847640:intron 50.0%
GAAGATGATGAGATGCCTGG+TGG - chr5_4:30100037-30100056 Msa0847640:intron 50.0%
TGTACTCTTTGAGCACCACC+AGG + chr5_4:30100055-30100074 None:intergenic 50.0%
GCGGAGAATTGCTCCGATCA+AGG + chr5_4:30094531-30094550 None:intergenic 55.0%
TGCAGAAGCGAATTCCGGTG+CGG + chr5_4:30094557-30094576 None:intergenic 55.0%
GGAATTCGCTTCTGCAGCTC+TGG - chr5_4:30094561-30094580 Msa0847640:three_prime_UTR 55.0%
ACGATGGGAGAAATAGCGCG+TGG - chr5_4:30095284-30095303 Msa0847640:intron 55.0%
GGCCTACCTGTTGCAACTCT+AGG + chr5_4:30095709-30095728 None:intergenic 55.0%
GCCTGCACTCTCAGGTACAA+CGG + chr5_4:30096260-30096279 None:intergenic 55.0%
!! CAGAAAGTGCCTGCACTCTC+AGG + chr5_4:30096268-30096287 None:intergenic 55.0%
TGCAGCTGGAGCTGATAGTC+CGG - chr5_4:30098613-30098632 Msa0847640:intron 55.0%
GGCATCTTCATCATCGCCGA+AGG + chr5_4:30098642-30098661 None:intergenic 55.0%
CATGGAGCTGGATGAGTGTG+AGG - chr5_4:30099940-30099959 Msa0847640:intron 55.0%
AAGGCAGCAAGCTCAGCAAG+AGG - chr5_4:30100006-30100025 Msa0847640:intron 55.0%
GAAGCGAATTCCGGTGCGGT+CGG + chr5_4:30094553-30094572 None:intergenic 60.0%
! GCCGTTGTACCTGAGAGTGC+AGG - chr5_4:30096256-30096275 Msa0847640:intron 60.0%
CCTTTCCGACCTCTGGTGCT+TGG + chr5_4:30098686-30098705 None:intergenic 60.0%
CCAAGCACCAGAGGTCGGAA+AGG - chr5_4:30098683-30098702 Msa0847640:intron 60.0%
CAATTCTCCGCCGACCGCAC+CGG - chr5_4:30094540-30094559 Msa0847640:three_prime_UTR 65.0%
! GGAGCGTTCGACGCGCTTCA+AGG - chr5_4:30098404-30098423 Msa0847640:intron 65.0%
GCGAATTCCGGTGCGGTCGG+CGG + chr5_4:30094550-30094569 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 30094085 30100391 30094085 ID=Msa0847640;Name=Msa0847640
chr5_4 mRNA 30094085 30100391 30094085 ID=Msa0847640-mRNA-1;Parent=Msa0847640;Name=Msa0847640-mRNA-1;_AED=0.08;_eAED=0.10;_QI=0|0.78|0.8|0.93|0.5|0.53|15|1770|209
chr5_4 exon 30094085 30094699 30094085 ID=Msa0847640-mRNA-1:exon:11040;Parent=Msa0847640-mRNA-1
chr5_4 exon 30095109 30095215 30095109 ID=Msa0847640-mRNA-1:exon:11039;Parent=Msa0847640-mRNA-1
chr5_4 exon 30095299 30095387 30095299 ID=Msa0847640-mRNA-1:exon:11038;Parent=Msa0847640-mRNA-1
chr5_4 exon 30095481 30095880 30095481 ID=Msa0847640-mRNA-1:exon:11037;Parent=Msa0847640-mRNA-1
chr5_4 exon 30096012 30096083 30096012 ID=Msa0847640-mRNA-1:exon:11036;Parent=Msa0847640-mRNA-1
chr5_4 exon 30097200 30097275 30097200 ID=Msa0847640-mRNA-1:exon:11035;Parent=Msa0847640-mRNA-1
chr5_4 exon 30097395 30097463 30097395 ID=Msa0847640-mRNA-1:exon:11034;Parent=Msa0847640-mRNA-1
chr5_4 exon 30097552 30097740 30097552 ID=Msa0847640-mRNA-1:exon:11033;Parent=Msa0847640-mRNA-1
chr5_4 exon 30098035 30098244 30098035 ID=Msa0847640-mRNA-1:exon:11032;Parent=Msa0847640-mRNA-1
chr5_4 exon 30098729 30098893 30098729 ID=Msa0847640-mRNA-1:exon:11031;Parent=Msa0847640-mRNA-1
chr5_4 exon 30098991 30099041 30098991 ID=Msa0847640-mRNA-1:exon:11030;Parent=Msa0847640-mRNA-1
chr5_4 exon 30099145 30099302 30099145 ID=Msa0847640-mRNA-1:exon:11029;Parent=Msa0847640-mRNA-1
chr5_4 exon 30099381 30099472 30099381 ID=Msa0847640-mRNA-1:exon:11028;Parent=Msa0847640-mRNA-1
chr5_4 exon 30100155 30100216 30100155 ID=Msa0847640-mRNA-1:exon:11027;Parent=Msa0847640-mRNA-1
chr5_4 exon 30100347 30100391 30100347 ID=Msa0847640-mRNA-1:exon:11026;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30100347 30100391 30100347 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30100155 30100216 30100155 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30099381 30099472 30099381 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30099145 30099302 30099145 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30098991 30099041 30098991 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30098729 30098893 30098729 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 CDS 30098188 30098244 30098188 ID=Msa0847640-mRNA-1:cds;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30098035 30098187 30098035 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30097552 30097740 30097552 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30097395 30097463 30097395 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30097200 30097275 30097200 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30096012 30096083 30096012 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30095481 30095880 30095481 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30095299 30095387 30095299 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30095109 30095215 30095109 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
chr5_4 three_prime_UTR 30094085 30094699 30094085 ID=Msa0847640-mRNA-1:three_prime_utr;Parent=Msa0847640-mRNA-1
Gene Sequence

>Msa0847640

TTGCTTTGGTTTTGTTGTAAGCCTGTTTTTCATCTTCTTCTTCAGGTGTCTGAGGTAGATAGTAGAAGATTGAATTGTGAAGAGTATGGTGAGTTTTGTTTGATTGGTGTAATTACTGTTTCTTCGGACAAAGGTATTTGTGGTGGAATCAACTCCACGTCTGTCAAGATAAGCAGGGCCTTGAGCAAGTTGAATTCTGTTATGGAGCATCACAAAGAGATAATTAAGAGTGGGTGTCATAAGATTTTGGAAGGAATCAATTCAATGTCTTTGATGAATTTAGATGACCAGCAAGTTCACTCCTTAATGACGATGGGAGAAATAGCGCGTGGAGTTGTTGTTGGTCGAGATAGAAAGGTGACTAAGAATGACGTGATTACTGCCTTGCAATACAGCATTGAGAAAATGGTGTGTGCATCTAGAGATATTGTTCATATGCTAAGTAGGATGTCCTATGAAGGCTTGCATGACCAGAGGATGCATTTAGTATTGAGGATGAAAGAAATTGCTCATGGGATTGTTGAGAAGCAACCTAGAGTTGCAACAGGTAGGCCTGAGTTACTTGCCGACCAATTCACTCTCAGTATTTGCGCTGCAGCCGTTGTACCTGAGAGTGCAGGCACTTTCTGA
Protein sequence

>Msa0847640

LLWFCCKPVFHLLLQVSEVDSRRLNCEEYGEFCLIGVITVSSDKGICGGINSTSVKISRALSKLNSVMEHHKEIIKSGCHKILEGINSMSLMNLDDQQVHSLMTMGEIARGVVVGRDRKVTKNDVITALQYSIEKMVCASRDIVHMLSRMSYEGLHDQRMHLVLRMKEIAHGIVEKQPRVATGRPELLADQFTLSICAAAVVPESAGTF*