AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0849450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACAATGATAATGTGTATT+TGG 0.176865 5_4:-36494076 None:intergenic
ATTAAACTTGGAAATGAATT+AGG 0.210390 5_4:+36493274 Msa0849450:CDS
TAATTCATTTCCAAGTTTAA+TGG 0.234342 5_4:-36493272 None:intergenic
ATCCGCCGTGTTCGTTTGAA+TGG 0.359236 5_4:+36491472 Msa0849450:CDS
TAGGTACCACCGTCGCTTCT+AGG 0.379077 5_4:-36491555 None:intergenic
CAAAATGATGCCATTAAACT+TGG 0.402093 5_4:+36493262 Msa0849450:CDS
CAGGAGAGCACGTGTCTTTG+TGG 0.456160 5_4:+36493207 Msa0849450:intron
CTAATATCACGATCTGATAG+AGG 0.463081 5_4:-36491529 None:intergenic
GGCTACTCTTCCTTCCTGCC+AGG 0.465761 5_4:-36493237 None:intergenic
ACACGGCGGATCCACGGTGG+CGG 0.489892 5_4:-36491460 None:intergenic
CTTCATCTCATAGTTTCTCA+AGG 0.506698 5_4:-36494001 None:intergenic
CTTGAGAAACTATGAGATGA+AGG 0.518610 5_4:+36494002 Msa0849450:CDS
ACGATCTGATAGAGGGTTGT+CGG 0.521070 5_4:-36491521 None:intergenic
CACGGCGGATCCACGGTGGC+GGG 0.526481 5_4:-36491459 None:intergenic
TGTCTTTGTGGAAGTAGTCC+TGG 0.529821 5_4:+36493219 Msa0849450:CDS
CGCCGTGTTCGTTTGAATGG+AGG 0.537898 5_4:+36491475 Msa0849450:CDS
TAGAGGGTTGTCGGTAGTAG+TGG 0.576250 5_4:-36491512 None:intergenic
TTTGTGGAAGTAGTCCTGGC+AGG 0.584999 5_4:+36493223 Msa0849450:CDS
AAACGAACACGGCGGATCCA+CGG 0.597311 5_4:-36491466 None:intergenic
TGGAAGTAGTCCTGGCAGGA+AGG 0.600387 5_4:+36493227 Msa0849450:CDS
TAATATCACGATCTGATAGA+GGG 0.623312 5_4:-36491528 None:intergenic
GTCCTCCATTCAAACGAACA+CGG 0.653128 5_4:-36491477 None:intergenic
AGCTGTCAAAGCTACTGATG+AGG 0.663227 5_4:+36494025 Msa0849450:CDS
TATTAGACGCCTAGAAGCGA+CGG 0.676167 5_4:+36491546 Msa0849450:CDS
TAGACGCCTAGAAGCGACGG+TGG 0.706943 5_4:+36491549 Msa0849450:CDS
CGAACACGGCGGATCCACGG+TGG 0.751314 5_4:-36491463 None:intergenic
CTCCATTCAAACGAACACGG+CGG 0.804040 5_4:-36491474 None:intergenic
GTTCATTTATCCCGCCACCG+TGG 0.805560 5_4:+36491449 Msa0849450:CDS
GGTGGCGGGATAAATGAACG+CGG 0.806439 5_4:-36491445 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAAATTATTTAAT+TGG + chr5_4:36495176-36495195 Msa0849450:intron 0.0%
!!! ATTAATTTTATTAATTTTAT+TGG + chr5_4:36495543-36495562 Msa0849450:intron 0.0%
!! AGAATATATTGTTAATATTT+AGG + chr5_4:36492835-36492854 Msa0849450:intron 10.0%
!!! ATTGGTAAAATTATAATTTT+TGG + chr5_4:36494369-36494388 Msa0849450:intron 10.0%
!! GAAATAAAATGAAAAAAATT+TGG - chr5_4:36494537-36494556 None:intergenic 10.0%
!! AAATGAAAAATAAAAAAGGA+CGG + chr5_4:36491985-36492004 Msa0849450:intron 15.0%
!! ATGAATAAATAATAATGGTT+AGG + chr5_4:36492030-36492049 Msa0849450:intron 15.0%
!! TGAATAAATAATAATGGTTA+GGG + chr5_4:36492031-36492050 Msa0849450:intron 15.0%
!!! TTAATATTTAGGCTTTATTT+GGG + chr5_4:36492846-36492865 Msa0849450:intron 15.0%
!! AACATAGTTTGAAAAAAAAA+GGG - chr5_4:36493055-36493074 None:intergenic 15.0%
!! AAACATAGTTTGAAAAAAAA+AGG - chr5_4:36493056-36493075 None:intergenic 15.0%
!!! TTTTTTTTATCTGTTTTTTG+TGG + chr5_4:36493330-36493349 Msa0849450:intron 15.0%
!! TATTTCAATTGTTAAATGAA+AGG - chr5_4:36493842-36493861 None:intergenic 15.0%
!! TGAAGCAAAAATTAATAAAA+TGG + chr5_4:36493895-36493914 Msa0849450:intron 15.0%
!!! ATTTAATTTAAATATGTGTG+TGG - chr5_4:36495162-36495181 None:intergenic 15.0%
!! ATTGGTAAAATTATAATTTC+TGG + chr5_4:36495584-36495603 Msa0849450:intron 15.0%
!!! TATTATTTTTAACTTGCAAT+TGG + chr5_4:36496014-36496033 Msa0849450:intron 15.0%
!!! TTTTAAGTTTTGAACCTAAA+AGG + chr5_4:36491764-36491783 Msa0849450:intron 20.0%
!!! AGTATAATAACAAACCTTTT+AGG - chr5_4:36491781-36491800 None:intergenic 20.0%
!! TGTTATTATACTTGTAACAT+AGG + chr5_4:36491789-36491808 Msa0849450:intron 20.0%
!! AATGAAAAATAAAAAAGGAC+GGG + chr5_4:36491986-36492005 Msa0849450:intron 20.0%
!! ATCTAAGTTATACATGATTT+AGG + chr5_4:36492142-36492161 Msa0849450:intron 20.0%
!! AGTATGAAATGTTAACTTTA+TGG + chr5_4:36492729-36492748 Msa0849450:intron 20.0%
!!! GTTAATATTTAGGCTTTATT+TGG + chr5_4:36492845-36492864 Msa0849450:intron 20.0%
!!! TAATATTTAGGCTTTATTTG+GGG + chr5_4:36492847-36492866 Msa0849450:intron 20.0%
!!! TGAATACTTTTGTTTTTTAC+TGG + chr5_4:36492893-36492912 Msa0849450:intron 20.0%
!!! GAATACTTTTGTTTTTTACT+GGG + chr5_4:36492894-36492913 Msa0849450:intron 20.0%
!!! TTTGTAACATTTTTTAGCTA+TGG - chr5_4:36493083-36493102 None:intergenic 20.0%
!! TAATTCATTTCCAAGTTTAA+TGG - chr5_4:36493275-36493294 None:intergenic 20.0%
!! ATTAAACTTGGAAATGAATT+AGG + chr5_4:36493274-36493293 Msa0849450:CDS 20.0%
!!! TTACAATGATAATGTGTATT+TGG - chr5_4:36494079-36494098 None:intergenic 20.0%
!!! TTATTATTTTTAGCTTGCAA+TGG + chr5_4:36494205-36494224 Msa0849450:intron 20.0%
!!! TATTATTTTTAGCTTGCAAT+GGG + chr5_4:36494206-36494225 Msa0849450:intron 20.0%
!!! AAAAATGGGTAATGTTTTTT+CGG + chr5_4:36494414-36494433 Msa0849450:intron 20.0%
!! ATTCAATTGATGAAATGAAA+AGG - chr5_4:36494695-36494714 None:intergenic 20.0%
!! AGAAGTTTGAACAAATAAAT+CGG - chr5_4:36495132-36495151 None:intergenic 20.0%
!! AACATGATTAACCATTATAA+TGG + chr5_4:36495310-36495329 Msa0849450:intron 20.0%
!! TTATATGTAAGGAAAATATG+AGG - chr5_4:36495427-36495446 None:intergenic 20.0%
!! TCATTCAATCATTATATGTA+AGG - chr5_4:36495438-36495457 None:intergenic 20.0%
! ATGAAAAATAAAAAAGGACG+GGG + chr5_4:36491987-36492006 Msa0849450:intron 25.0%
!!! TCACAGTTTTTAAGTCAAAA+AGG - chr5_4:36492094-36492113 None:intergenic 25.0%
!! TTAATACTATGTTTTGCCAT+TGG + chr5_4:36492308-36492327 Msa0849450:intron 25.0%
! GAAAATGAACTCAAAGTTAA+GGG - chr5_4:36492393-36492412 None:intergenic 25.0%
! AGAAAATGAACTCAAAGTTA+AGG - chr5_4:36492394-36492413 None:intergenic 25.0%
!! ATTTTCCTAAGAGTTTAAGA+AGG + chr5_4:36492427-36492446 Msa0849450:intron 25.0%
!! GAAAATATGAAGAGTATAAG+AGG - chr5_4:36492668-36492687 None:intergenic 25.0%
! AAATGTTAACTTTATGGACA+TGG + chr5_4:36492735-36492754 Msa0849450:intron 25.0%
! TTCTTACATGTAGTAGTATT+TGG - chr5_4:36492819-36492838 None:intergenic 25.0%
!!! TTTTTTCTTCCCACATTTTA+AGG + chr5_4:36492975-36492994 Msa0849450:intron 25.0%
! AGATGATTTACAAATAGTCT+TGG - chr5_4:36493112-36493131 None:intergenic 25.0%
!! ATCATCTTTTGTGAAACTTT+TGG + chr5_4:36493125-36493144 Msa0849450:intron 25.0%
! AGAAGACAAAAAGTAAGATA+AGG + chr5_4:36493173-36493192 Msa0849450:intron 25.0%
! AGATAAGGTTATAAAAGTTC+AGG + chr5_4:36493188-36493207 Msa0849450:intron 25.0%
!! CAAAACTCAAAGTATTAACA+AGG - chr5_4:36493521-36493540 None:intergenic 25.0%
! TTCAAAAGCAATGTTGTAAA+GGG - chr5_4:36493573-36493592 None:intergenic 25.0%
! ATTCAAAAGCAATGTTGTAA+AGG - chr5_4:36493574-36493593 None:intergenic 25.0%
!! AGAATTTGAGTTAAAGTCAA+AGG - chr5_4:36493610-36493629 None:intergenic 25.0%
!! AAGTTTTCAAAAGATGGTTA+TGG + chr5_4:36493709-36493728 Msa0849450:intron 25.0%
!! ATAAGATATTAGCTTCAACA+AGG - chr5_4:36493938-36493957 None:intergenic 25.0%
! TTGTTGAAGCTAATATCTTA+TGG + chr5_4:36493937-36493956 Msa0849450:intron 25.0%
! TACTAGGAGAGTTTATTATA+AGG - chr5_4:36494272-36494291 None:intergenic 25.0%
!!! TAATTTTTGGAGACATCTTA+TGG + chr5_4:36494382-36494401 Msa0849450:intron 25.0%
!!! TTATGGATTAGACAGAAAAA+TGG + chr5_4:36494399-36494418 Msa0849450:intron 25.0%
!!! TATGGATTAGACAGAAAAAT+GGG + chr5_4:36494400-36494419 Msa0849450:intron 25.0%
!! AAAAATTTGGATACCCATTT+TGG - chr5_4:36494524-36494543 None:intergenic 25.0%
! TATTCATCGTTATCAAAAGA+AGG - chr5_4:36494578-36494597 None:intergenic 25.0%
!!! TAACGATGAATACCTTTTTT+CGG + chr5_4:36494586-36494605 Msa0849450:intron 25.0%
! AGAAAATAAACTCAAAGTGA+AGG - chr5_4:36494882-36494901 None:intergenic 25.0%
!!! AAGTTTTCAAATGTTGGTTA+TGG + chr5_4:36495255-36495274 Msa0849450:intron 25.0%
!! TACTATGAGAGTTTTCTATA+AGG - chr5_4:36495365-36495384 None:intergenic 25.0%
TAATATCACGATCTGATAGA+GGG - chr5_4:36491531-36491550 None:intergenic 30.0%
!!! ATCTTTTTTCATCTATGCTC+CGG - chr5_4:36491633-36491652 None:intergenic 30.0%
!! ACTGTTTTAGATCTAAGCTT+TGG - chr5_4:36491662-36491681 None:intergenic 30.0%
GATTATGCATGTACTTTCAT+TGG + chr5_4:36491725-36491744 Msa0849450:intron 30.0%
! CATTAGGTTCAATTGATTCA+AGG - chr5_4:36491845-36491864 None:intergenic 30.0%
GAATCAATTGAACCTAATGT+CGG + chr5_4:36491846-36491865 Msa0849450:intron 30.0%
AATCAATTGAACCTAATGTC+GGG + chr5_4:36491847-36491866 Msa0849450:intron 30.0%
!!! ATCTCATTTTGAAGCTGTAT+GGG - chr5_4:36491953-36491972 None:intergenic 30.0%
!!! AATCTCATTTTGAAGCTGTA+TGG - chr5_4:36491954-36491973 None:intergenic 30.0%
GAAAATCTAAGAACACGATA+GGG - chr5_4:36492363-36492382 None:intergenic 30.0%
ATCAACCTTCTTAAACTCTT+AGG - chr5_4:36492435-36492454 None:intergenic 30.0%
!! AAGGTTGATTTCTTTTGAAC+AGG + chr5_4:36492446-36492465 Msa0849450:intron 30.0%
! ATTTCTTTTGAACAGGTGTT+TGG + chr5_4:36492453-36492472 Msa0849450:intron 30.0%
! TTTCTTTTGAACAGGTGTTT+GGG + chr5_4:36492454-36492473 Msa0849450:intron 30.0%
TTAACTTTATGGACATGGTA+AGG + chr5_4:36492740-36492759 Msa0849450:intron 30.0%
TAACTTTATGGACATGGTAA+GGG + chr5_4:36492741-36492760 Msa0849450:intron 30.0%
TTACATGTAGTAGTATTTGG+TGG - chr5_4:36492816-36492835 None:intergenic 30.0%
!!! ACTTTTGTTTTTTACTGGGA+AGG + chr5_4:36492898-36492917 Msa0849450:intron 30.0%
AAATACAGCTATAGCAGAAA+AGG - chr5_4:36493001-36493020 None:intergenic 30.0%
! GTTTAATGGCATCATTTTGA+TGG - chr5_4:36493261-36493280 None:intergenic 30.0%
CAAAATGATGCCATTAAACT+TGG + chr5_4:36493262-36493281 Msa0849450:CDS 30.0%
!!! TTATCTGTTTTTTGTGGAAG+AGG + chr5_4:36493336-36493355 Msa0849450:intron 30.0%
!! TACAACATTGCTTTTGAATC+TGG + chr5_4:36493575-36493594 Msa0849450:intron 30.0%
TTTGTTACACAAGGTTTCAT+GGG - chr5_4:36493653-36493672 None:intergenic 30.0%
!! CCATCTTTTGAAAACTTTTG+TGG - chr5_4:36493706-36493725 None:intergenic 30.0%
! CCACAAAAGTTTTCAAAAGA+TGG + chr5_4:36493703-36493722 Msa0849450:intron 30.0%
TTTAATCTACACAAATCTGC+AGG + chr5_4:36493973-36493992 Msa0849450:intron 30.0%
AATGAGAAAGAACACAAAAG+TGG - chr5_4:36494177-36494196 None:intergenic 30.0%
TCTTACAATAGACACATGTT+TGG + chr5_4:36494342-36494361 Msa0849450:intron 30.0%
GAAAAAGGAATACCGAAAAA+AGG - chr5_4:36494601-36494620 None:intergenic 30.0%
CACAAATTCCACATAATCAA+GGG - chr5_4:36494752-36494771 None:intergenic 30.0%
TTAATACATTGTTCTGCCAT+TGG + chr5_4:36494827-36494846 Msa0849450:intron 30.0%
TAAAAGCAATACAAAGCCAA+TGG - chr5_4:36494846-36494865 None:intergenic 30.0%
AGTGTTGTAAAGGGAAAATT+TGG - chr5_4:36495064-36495083 None:intergenic 30.0%
TTTGGAAGTAGTGTTGTAAA+GGG - chr5_4:36495073-36495092 None:intergenic 30.0%
ATTTGGAAGTAGTGTTGTAA+AGG - chr5_4:36495074-36495093 None:intergenic 30.0%
TACAACACTACTTCCAAATT+TGG + chr5_4:36495075-36495094 Msa0849450:intron 30.0%
AAGGAAACAACAACCAAATT+TGG - chr5_4:36495091-36495110 None:intergenic 30.0%
!! TAAATCGGATTTTGAGTCAT+AGG - chr5_4:36495117-36495136 None:intergenic 30.0%
! GAAAACTTTTGAGCATATGT+TGG - chr5_4:36495243-36495262 None:intergenic 30.0%
! GCTCAAAAGTTTTCAAATGT+TGG + chr5_4:36495249-36495268 Msa0849450:intron 30.0%
AGGTATGGCAAAAGAAAAAT+TGG - chr5_4:36495345-36495364 None:intergenic 30.0%
! TGAGAGTTTTCTATAAGGTA+TGG - chr5_4:36495360-36495379 None:intergenic 30.0%
GAAAATATGAGGAATCCAAA+AGG - chr5_4:36495416-36495435 None:intergenic 30.0%
AGACACATGTTTAGTAACAT+TGG + chr5_4:36495566-36495585 Msa0849450:intron 30.0%
! ACAGTGATAATGTGCATTAT+GGG - chr5_4:36495884-36495903 None:intergenic 30.0%
! TACAGTGATAATGTGCATTA+TGG - chr5_4:36495885-36495904 None:intergenic 30.0%
CTAATATCACGATCTGATAG+AGG - chr5_4:36491532-36491551 None:intergenic 35.0%
! AGCTTAGATCTAAAACAGTG+AGG + chr5_4:36491663-36491682 Msa0849450:intron 35.0%
! GCTTAGATCTAAAACAGTGA+GGG + chr5_4:36491664-36491683 Msa0849450:intron 35.0%
! TCAATTGATTCAAGGTGGTT+AGG - chr5_4:36491837-36491856 None:intergenic 35.0%
! TAGGTTCAATTGATTCAAGG+TGG - chr5_4:36491842-36491861 None:intergenic 35.0%
!! TAGAGTCTGAGATTGAGTTT+TGG + chr5_4:36491893-36491912 Msa0849450:intron 35.0%
!!! AGTTTTGGAACGTCTTGTTT+TGG + chr5_4:36491908-36491927 Msa0849450:intron 35.0%
!! TTGGTAATACACTCCTCTTT+TGG + chr5_4:36491927-36491946 Msa0849450:intron 35.0%
AAATAAAAAAGGACGGGGAA+TGG + chr5_4:36491992-36492011 Msa0849450:intron 35.0%
AATAAAAAAGGACGGGGAAT+GGG + chr5_4:36491993-36492012 Msa0849450:intron 35.0%
GGAAAATCTAAGAACACGAT+AGG - chr5_4:36492364-36492383 None:intergenic 35.0%
ACTCAAAGTTAAGGGAAAGT+GGG - chr5_4:36492385-36492404 None:intergenic 35.0%
AACTCAAAGTTAAGGGAAAG+TGG - chr5_4:36492386-36492405 None:intergenic 35.0%
! TTCTTTTGAACAGGTGTTTG+GGG + chr5_4:36492455-36492474 Msa0849450:intron 35.0%
AAATTCAGGAAACCCATTCA+TGG + chr5_4:36492578-36492597 Msa0849450:intron 35.0%
CATTTCATACTGTTCGTTTC+AGG - chr5_4:36492720-36492739 None:intergenic 35.0%
GGATATGCAAGTGAATAAGA+TGG + chr5_4:36492776-36492795 Msa0849450:intron 35.0%
!! ATTTAGGCTTTATTTGGGGA+AGG + chr5_4:36492851-36492870 Msa0849450:intron 35.0%
CTTTATATTCCTGACCTACA+CGG + chr5_4:36492942-36492961 Msa0849450:intron 35.0%
CAGAAAAGGCCTTAAAATGT+GGG - chr5_4:36492987-36493006 None:intergenic 35.0%
AATTCGTTGCAAACTTCAGT+TGG + chr5_4:36493401-36493420 Msa0849450:intron 35.0%
ATTCGTTGCAAACTTCAGTT+GGG + chr5_4:36493402-36493421 Msa0849450:intron 35.0%
! GTTTGTTACACAAGGTTTCA+TGG - chr5_4:36493654-36493673 None:intergenic 35.0%
GACTAAGAGAGTCGATTAAA+AGG - chr5_4:36493769-36493788 None:intergenic 35.0%
CTTCATCTCATAGTTTCTCA+AGG - chr5_4:36494004-36494023 None:intergenic 35.0%
CTTGAGAAACTATGAGATGA+AGG + chr5_4:36494002-36494021 Msa0849450:CDS 35.0%
AGAAAGAACACAAAAGTGGA+AGG - chr5_4:36494173-36494192 None:intergenic 35.0%
!!! TTTTAGCTTGCAATGGGTTA+TGG + chr5_4:36494212-36494231 Msa0849450:intron 35.0%
! TTCACGTTCACACTTTTACT+AGG - chr5_4:36494288-36494307 None:intergenic 35.0%
AAAAATATGCAGAGTCCAAG+AGG - chr5_4:36494323-36494342 None:intergenic 35.0%
! AGACACATGTTTGGTAACAT+TGG + chr5_4:36494351-36494370 Msa0849450:intron 35.0%
!!! ATTTTGGTTCGATTCTTGCA+TGG - chr5_4:36494508-36494527 None:intergenic 35.0%
ATGCAAGAATCGAACCAAAA+TGG + chr5_4:36494507-36494526 Msa0849450:intron 35.0%
TGCAAGAATCGAACCAAAAT+GGG + chr5_4:36494508-36494527 Msa0849450:intron 35.0%
CTATGCAGCAGTTATGAAAA+AGG - chr5_4:36494616-36494635 None:intergenic 35.0%
CTTAATCATTATGATGCCCT+TGG - chr5_4:36494660-36494679 None:intergenic 35.0%
ACGTTATTCCCTTGATTATG+TGG + chr5_4:36494741-36494760 Msa0849450:intron 35.0%
CCACAAATTCCACATAATCA+AGG - chr5_4:36494753-36494772 None:intergenic 35.0%
CCTTGATTATGTGGAATTTG+TGG + chr5_4:36494750-36494769 Msa0849450:intron 35.0%
! TTTCTTTTGATCAGGTGGTT+AGG + chr5_4:36494946-36494965 Msa0849450:intron 35.0%
!! GATTTTGAGTCATAGGTCAA+AGG - chr5_4:36495110-36495129 None:intergenic 35.0%
! GAATTCCATTGTATGCCTTT+TGG + chr5_4:36495398-36495417 Msa0849450:intron 35.0%
TTCATCTCTGTGTGTGGTTA+AGG - chr5_4:36491596-36491615 None:intergenic 40.0%
ATAAAAAAGGACGGGGAATG+GGG + chr5_4:36491994-36492013 Msa0849450:intron 40.0%
AAGTCACAGGTGACAAATTC+AGG + chr5_4:36492564-36492583 Msa0849450:intron 40.0%
TACAGAAGGTCCATCTAGAT+GGG - chr5_4:36492635-36492654 None:intergenic 40.0%
TTACAGAAGGTCCATCTAGA+TGG - chr5_4:36492636-36492655 None:intergenic 40.0%
GAGGAAAGAGAACTTACAGA+AGG - chr5_4:36492649-36492668 None:intergenic 40.0%
GACATGGTAAGGGTTAGAAA+AGG + chr5_4:36492751-36492770 Msa0849450:intron 40.0%
TGGTAAGGGTTAGAAAAGGA+TGG + chr5_4:36492755-36492774 Msa0849450:intron 40.0%
!! GCTTTATTTGGGGAAGGTAA+GGG + chr5_4:36492857-36492876 Msa0849450:intron 40.0%
TGGGAAGGTTGAACTTGATA+TGG + chr5_4:36492913-36492932 Msa0849450:intron 40.0%
GGGAAGGTTGAACTTGATAT+GGG + chr5_4:36492914-36492933 Msa0849450:intron 40.0%
GGAAGGTTGAACTTGATATG+GGG + chr5_4:36492915-36492934 Msa0849450:intron 40.0%
AAAACAAAGAGCTACCGTGT+AGG - chr5_4:36492959-36492978 None:intergenic 40.0%
GCAGAAAAGGCCTTAAAATG+TGG - chr5_4:36492988-36493007 None:intergenic 40.0%
GCCATATCTGACATACAATC+TGG + chr5_4:36493029-36493048 Msa0849450:intron 40.0%
GCCAGATTGTATGTCAGATA+TGG - chr5_4:36493033-36493052 None:intergenic 40.0%
! GAAACTTTTGGCATGTTTGC+AGG + chr5_4:36493137-36493156 Msa0849450:intron 40.0%
!!! TCTGTTTTTTGTGGAAGAGG+TGG + chr5_4:36493339-36493358 Msa0849450:intron 40.0%
! TTTTGTGGAAGAGGTGGAAA+TGG + chr5_4:36493345-36493364 Msa0849450:intron 40.0%
TTCGTTGCAAACTTCAGTTG+GGG + chr5_4:36493403-36493422 Msa0849450:intron 40.0%
! TTGATGCATCGTTTTGCCTT+TGG + chr5_4:36493457-36493476 Msa0849450:intron 40.0%
!! AAGGGATAATTTGCTACCTC+TGG - chr5_4:36493555-36493574 None:intergenic 40.0%
! GTGAGTGTGTTTGTTACACA+AGG - chr5_4:36493662-36493681 None:intergenic 40.0%
AGAGAGTCGATTAAAAGGCA+TGG - chr5_4:36493764-36493783 None:intergenic 40.0%
GAATTCCATTGCATGCTTCT+AGG + chr5_4:36493802-36493821 Msa0849450:intron 40.0%
GAATTCCATTGTATGCCTCT+TGG + chr5_4:36494305-36494324 Msa0849450:intron 40.0%
! CTGCTGCATAGATCTTTTTG+TGG + chr5_4:36494625-36494644 Msa0849450:intron 40.0%
! TTTTGTGGCAGCTAAATCCA+AGG + chr5_4:36494640-36494659 Msa0849450:intron 40.0%
TTTGTGGCAGCTAAATCCAA+GGG + chr5_4:36494641-36494660 Msa0849450:intron 40.0%
TCTCGCAACAGTATAAGAGA+CGG + chr5_4:36494919-36494938 Msa0849450:intron 40.0%
!! CTCAAGCTTGTTGCATATGT+TGG + chr5_4:36494970-36494989 Msa0849450:intron 40.0%
TGTTAGTGTTGCAAAGTCAG+AGG + chr5_4:36495036-36495055 Msa0849450:intron 40.0%
GGCTTGCAAGTCCATTATAA+TGG - chr5_4:36495324-36495343 None:intergenic 40.0%
! GGAATCCAAAAGGCATACAA+TGG - chr5_4:36495406-36495425 None:intergenic 40.0%
TTTGCGGAATTGTCTGATGA+AGG + chr5_4:36495803-36495822 Msa0849450:intron 40.0%
ATCACATCTGCAGATTTGTG+TGG - chr5_4:36495831-36495850 None:intergenic 40.0%
ACACAAATCTGCAGATGTGA+TGG + chr5_4:36495830-36495849 Msa0849450:intron 40.0%
CACAAATCTGCAGATGTGAT+GGG + chr5_4:36495831-36495850 Msa0849450:intron 40.0%
ACAAATCTGCAGATGTGATG+GGG + chr5_4:36495832-36495851 Msa0849450:intron 40.0%
GTCCTCCATTCAAACGAACA+CGG - chr5_4:36491480-36491499 None:intergenic 45.0%
ACGATCTGATAGAGGGTTGT+CGG - chr5_4:36491524-36491543 None:intergenic 45.0%
TATTAGACGCCTAGAAGCGA+CGG + chr5_4:36491546-36491565 Msa0849450:CDS 45.0%
AAGGTCTTAGAAGCTGTCGT+AGG - chr5_4:36491577-36491596 None:intergenic 45.0%
! GCAGTTTTCATCTCTGTGTG+TGG - chr5_4:36491602-36491621 None:intergenic 45.0%
ATGAAAACTGCAGACGACAC+CGG + chr5_4:36491611-36491630 Msa0849450:intron 45.0%
GAACCTAATGTCGGGATTTG+CGG + chr5_4:36491855-36491874 Msa0849450:intron 45.0%
AACCTAATGTCGGGATTTGC+GGG + chr5_4:36491856-36491875 Msa0849450:intron 45.0%
AGAGAAGAACGCAAAGCCAA+TGG - chr5_4:36492327-36492346 None:intergenic 45.0%
GTTGAGGATGTTGGCATAGT+TGG + chr5_4:36492493-36492512 Msa0849450:intron 45.0%
! ATAGTTGGTGCTCCAATGCA+GGG + chr5_4:36492508-36492527 Msa0849450:intron 45.0%
! GAGTGTACTTTTCCCTGCAT+TGG - chr5_4:36492523-36492542 None:intergenic 45.0%
TGTTAGTGCTGCAAAGTCAC+AGG + chr5_4:36492551-36492570 Msa0849450:intron 45.0%
AGGAAACCCATTCATGGAGT+GGG + chr5_4:36492584-36492603 Msa0849450:intron 45.0%
ACCATTTCTCCCCATCTAGA+TGG + chr5_4:36492622-36492641 Msa0849450:intron 45.0%
TCCATCTAGATGGGGAGAAA+TGG - chr5_4:36492626-36492645 None:intergenic 45.0%
ACAGAAGGTCCATCTAGATG+GGG - chr5_4:36492634-36492653 None:intergenic 45.0%
!! GGCTTTATTTGGGGAAGGTA+AGG + chr5_4:36492856-36492875 Msa0849450:intron 45.0%
GGTTGAACTTGATATGGGGA+AGG + chr5_4:36492919-36492938 Msa0849450:intron 45.0%
TGTCTTTGTGGAAGTAGTCC+TGG + chr5_4:36493219-36493238 Msa0849450:CDS 45.0%
TCGTTGCAAACTTCAGTTGG+GGG + chr5_4:36493404-36493423 Msa0849450:intron 45.0%
GAGCAGAGACACAAATCCAA+AGG - chr5_4:36493476-36493495 None:intergenic 45.0%
! TTTTGCTAGTGCAAAGCCAG+AGG + chr5_4:36493536-36493555 Msa0849450:intron 45.0%
! CAAGTCCTAGAAGCATGCAA+TGG - chr5_4:36493810-36493829 None:intergenic 45.0%
AGCTGTCAAAGCTACTGATG+AGG + chr5_4:36494025-36494044 Msa0849450:CDS 45.0%
! AGAGTCCAAGAGGCATACAA+TGG - chr5_4:36494313-36494332 None:intergenic 45.0%
!!! ACGGCTGGTTTCTTTTGATC+AGG + chr5_4:36494938-36494957 Msa0849450:intron 45.0%
!! GCTGGTTTCTTTTGATCAGG+TGG + chr5_4:36494941-36494960 Msa0849450:intron 45.0%
TTCTTGTCGACATACCTGCT+TGG + chr5_4:36495765-36495784 Msa0849450:intron 45.0%
! AGACAATTCCGCAAAGCGTT+TGG - chr5_4:36495798-36495817 None:intergenic 45.0%
!! AATTAAATGAAAAATAAAAA+AGG + chr5_4:36491981-36492000 Msa0849450:intron 5.0%
!! AATAAATGAATAAATAATAA+TGG + chr5_4:36492025-36492044 Msa0849450:intron 5.0%
!!! AAGATTTTTTTTTTAAAAAA+GGG - chr5_4:36493436-36493455 None:intergenic 5.0%
!!! TAAGATTTTTTTTTTAAAAA+AGG - chr5_4:36493437-36493456 None:intergenic 5.0%
!! AATAATAACAATAATAATAT+AGG + chr5_4:36495466-36495485 Msa0849450:intron 5.0%
ATCCGCCGTGTTCGTTTGAA+TGG + chr5_4:36491472-36491491 Msa0849450:CDS 50.0%
CTCCATTCAAACGAACACGG+CGG - chr5_4:36491477-36491496 None:intergenic 50.0%
TAGAGGGTTGTCGGTAGTAG+TGG - chr5_4:36491515-36491534 None:intergenic 50.0%
TTCCCGCAAATCCCGACATT+AGG - chr5_4:36491861-36491880 None:intergenic 50.0%
GAAGCTGTATGGGCCAAAAG+AGG - chr5_4:36491943-36491962 None:intergenic 50.0%
! CATAGTTGGTGCTCCAATGC+AGG + chr5_4:36492507-36492526 Msa0849450:intron 50.0%
CAGGAAACCCATTCATGGAG+TGG + chr5_4:36492583-36492602 Msa0849450:intron 50.0%
GGAAACCCATTCATGGAGTG+GGG + chr5_4:36492585-36492604 Msa0849450:intron 50.0%
TCATGCCCCACTCCATGAAT+GGG - chr5_4:36492593-36492612 None:intergenic 50.0%
ATCATGCCCCACTCCATGAA+TGG - chr5_4:36492594-36492613 None:intergenic 50.0%
AAAGAGCTACCGTGTAGGTC+AGG - chr5_4:36492954-36492973 None:intergenic 50.0%
TTTGTGGAAGTAGTCCTGGC+AGG + chr5_4:36493223-36493242 Msa0849450:CDS 50.0%
CGTTGCAAACTTCAGTTGGG+GGG + chr5_4:36493405-36493424 Msa0849450:intron 50.0%
GCAACAGTATAAGAGACGGC+TGG + chr5_4:36494923-36494942 Msa0849450:intron 50.0%
GCTGCTATCCAAACGCTTTG+CGG + chr5_4:36495787-36495806 Msa0849450:intron 50.0%
!! GGTGGCGGGATAAATGAACG+CGG - chr5_4:36491448-36491467 None:intergenic 55.0%
GTTCATTTATCCCGCCACCG+TGG + chr5_4:36491449-36491468 Msa0849450:CDS 55.0%
AAACGAACACGGCGGATCCA+CGG - chr5_4:36491469-36491488 None:intergenic 55.0%
CGCCGTGTTCGTTTGAATGG+AGG + chr5_4:36491475-36491494 Msa0849450:CDS 55.0%
TAGGTACCACCGTCGCTTCT+AGG - chr5_4:36491558-36491577 None:intergenic 55.0%
! TTTGGGGTTAGAACCCACGC+TGG + chr5_4:36492471-36492490 Msa0849450:intron 55.0%
! GTTAGAACCCACGCTGGTTG+AGG + chr5_4:36492477-36492496 Msa0849450:intron 55.0%
CCAACATCCTCAACCAGCGT+GGG - chr5_4:36492487-36492506 None:intergenic 55.0%
! CAGGAGAGCACGTGTCTTTG+TGG + chr5_4:36493207-36493226 Msa0849450:intron 55.0%
TGGAAGTAGTCCTGGCAGGA+AGG + chr5_4:36493227-36493246 Msa0849450:CDS 55.0%
CGTTTGGATAGCAGCCAAGC+AGG - chr5_4:36495782-36495801 None:intergenic 55.0%
TAGACGCCTAGAAGCGACGG+TGG + chr5_4:36491549-36491568 Msa0849450:CDS 60.0%
! CCCACGCTGGTTGAGGATGT+TGG + chr5_4:36492484-36492503 Msa0849450:intron 60.0%
GCCAACATCCTCAACCAGCG+TGG - chr5_4:36492488-36492507 None:intergenic 60.0%
GGCTACTCTTCCTTCCTGCC+AGG - chr5_4:36493240-36493259 None:intergenic 60.0%
ACACGGCGGATCCACGGTGG+CGG - chr5_4:36491463-36491482 None:intergenic 70.0%
CGAACACGGCGGATCCACGG+TGG - chr5_4:36491466-36491485 None:intergenic 70.0%
CACGGCGGATCCACGGTGGC+GGG - chr5_4:36491462-36491481 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 36491445 36496060 36491445 ID=Msa0849450;Name=Msa0849450
chr5_4 mRNA 36491445 36496060 36491445 ID=Msa0849450-mRNA-1;Parent=Msa0849450;Name=Msa0849450-mRNA-1;_AED=0.42;_eAED=0.64;_QI=0|0.2|0.16|1|0|0|6|0|117
chr5_4 exon 36491445 36491570 36491445 ID=Msa0849450-mRNA-1:exon:12026;Parent=Msa0849450-mRNA-1
chr5_4 exon 36492468 36492476 36492468 ID=Msa0849450-mRNA-1:exon:12027;Parent=Msa0849450-mRNA-1
chr5_4 exon 36493159 36493184 36493159 ID=Msa0849450-mRNA-1:exon:12028;Parent=Msa0849450-mRNA-1
chr5_4 exon 36493210 36493295 36493210 ID=Msa0849450-mRNA-1:exon:12029;Parent=Msa0849450-mRNA-1
chr5_4 exon 36493995 36494094 36493995 ID=Msa0849450-mRNA-1:exon:12030;Parent=Msa0849450-mRNA-1
chr5_4 exon 36496057 36496060 36496057 ID=Msa0849450-mRNA-1:exon:12031;Parent=Msa0849450-mRNA-1
chr5_4 CDS 36491445 36491570 36491445 ID=Msa0849450-mRNA-1:cds;Parent=Msa0849450-mRNA-1
chr5_4 CDS 36492468 36492476 36492468 ID=Msa0849450-mRNA-1:cds;Parent=Msa0849450-mRNA-1
chr5_4 CDS 36493159 36493184 36493159 ID=Msa0849450-mRNA-1:cds;Parent=Msa0849450-mRNA-1
chr5_4 CDS 36493210 36493295 36493210 ID=Msa0849450-mRNA-1:cds;Parent=Msa0849450-mRNA-1
chr5_4 CDS 36493995 36494094 36493995 ID=Msa0849450-mRNA-1:cds;Parent=Msa0849450-mRNA-1
chr5_4 CDS 36496057 36496060 36496057 ID=Msa0849450-mRNA-1:cds;Parent=Msa0849450-mRNA-1
Gene Sequence

>Msa0849450

CCGCGTTCATTTATCCCGCCACCGTGGATCCGCCGTGTTCGTTTGAATGGAGGACATTTGTCGACGACCACTACTACCGACAACCCTCTATCAGATCGTGATATTAGACGCCTAGAAGCGACGGTGGTGTTTGGGGTAACAAATACAAAAGAAGACAAAAAGAGAGCACGTGTCTTTGTGGAAGTAGTCCTGGCAGGAAGGAAGAGTAGCCATCAAAATGATGCCATTAAACTTGGAAATGAATTAGGTGACACCTTGAGAAACTATGAGATGAAGGAGCTGTCAAAGCTACTGATGAGGACTTGTTTACATGCTTGCTTGCTGCTGTCCAAATACACATTATCATTAATA
Protein sequence

>Msa0849450

PRSFIPPPWIRRVRLNGGHLSTTTTTDNPLSDRDIRRLEATVVFGVTNTKEDKKRARVFVEVVLAGRKSSHQNDAIKLGNELGDTLRNYEMKELSKLLMRTCLHACLLLSKYTLSLI