AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0894920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCCTCCTTCAAGAATTC+TGG 0.172529 6_1:+81538960 Msa0894920:intron
TTCCCTCATGTAGTATTTAA+AGG 0.173459 6_1:-81539261 None:intergenic
TTACAATGATAATGTGTATT+TGG 0.176865 6_1:-81536785 None:intergenic
TACAGTGATAATGTGTATTA+TGG 0.178827 6_1:-81538531 None:intergenic
ATTAAACTTGGAAATGAATT+AGG 0.210390 6_1:+81535984 Msa0894920:CDS
TAATTCATTTCCAAGTTTAA+TGG 0.234342 6_1:-81535982 None:intergenic
CAGAATTTGCAGTGCAGCGC+AGG 0.294148 6_1:-81539354 None:intergenic
GTTGAGGATGTTGGCATAGT+TGG 0.363283 6_1:+81535204 Msa0894920:CDS
AGACAATTCCGCAAAGCGTT+TGG 0.379036 6_1:-81538444 None:intergenic
CTGCAGATGCAGATGTTGAT+AGG 0.392164 6_1:+81539374 Msa0894920:three_prime_UTR
CAAAATGATGCCATTAAACT+TGG 0.402093 6_1:+81535972 Msa0894920:CDS
CGAAAGAGCCTTGGAGAGAA+AGG 0.423381 6_1:+81539285 Msa0894920:three_prime_UTR
AGAAAGAGCCTTGGAGAGAA+AGG 0.426750 6_1:+81539330 Msa0894920:three_prime_UTR
AGATGTTGATAGGTTCATAT+AGG 0.436481 6_1:+81539384 Msa0894920:three_prime_UTR
GTTCCTCCTTCAAGAATTCT+GGG 0.442364 6_1:+81538961 Msa0894920:intron
ACAGTGATAATGTGTATTAT+GGG 0.445189 6_1:-81538530 None:intergenic
GCTGCTATCCAAACGCTTTG+CGG 0.447265 6_1:+81538436 Msa0894920:three_prime_UTR
CTCAAGCTTGTTGCATATGT+TGG 0.452344 6_1:+81537720 Msa0894920:CDS
CAACTAAGAGCTTATCTCAT+TGG 0.455962 6_1:-81539084 None:intergenic
CAGGAGAGCACGTGTCTTTG+TGG 0.456160 6_1:+81535917 Msa0894920:intron
CATAGTTGGTGCTCCAATGC+AGG 0.464430 6_1:+81535218 Msa0894920:CDS
GGCTACTCTTCCTTCCTGCC+AGG 0.465761 6_1:-81535947 None:intergenic
GTTAGAACCCACGCTGGTTG+AGG 0.491363 6_1:+81535188 Msa0894920:CDS
AAGCTCTTCCTTTCTCTCCA+AGG 0.495149 6_1:-81539293 None:intergenic
TTTGCGGAATTGTCTGATGA+AGG 0.504707 6_1:+81538452 Msa0894920:three_prime_UTR
CTTCATCTCATAGTTTCTCA+AGG 0.506698 6_1:-81536710 None:intergenic
TGAGGGAAGCGAAAGAGCCT+TGG 0.511561 6_1:+81539276 Msa0894920:three_prime_UTR
CGTTTGGATAGCAGCCAAGC+AGG 0.516208 6_1:-81538428 None:intergenic
CTTGAGAAACTATGAGATGA+AGG 0.518610 6_1:+81536711 Msa0894920:CDS
TTGGAGAGAAAGGAAGAGCT+TGG 0.523025 6_1:+81539295 Msa0894920:three_prime_UTR
CCCACGCTGGTTGAGGATGT+TGG 0.525901 6_1:+81535195 Msa0894920:CDS
GCGCAGGGCCTTTCTCTCCA+AGG 0.526608 6_1:-81539338 None:intergenic
TGTCTTTGTGGAAGTAGTCC+TGG 0.529821 6_1:+81535929 Msa0894920:CDS
TTGCCTTTAAATACTACATG+AGG 0.534140 6_1:+81539258 Msa0894920:three_prime_UTR
TTCTTGTCGACATACCTGCT+TGG 0.538298 6_1:+81538414 Msa0894920:three_prime_UTR
GTGAGAGAGAGAAAGAGCCT+TGG 0.551422 6_1:+81539321 Msa0894920:three_prime_UTR
AGAATTTGCAGTGCAGCGCA+GGG 0.576307 6_1:-81539353 None:intergenic
TTTGTGGAAGTAGTCCTGGC+AGG 0.584999 6_1:+81535933 Msa0894920:CDS
CAAAATGTGATGGCAAGGCA+AGG 0.585482 6_1:+81539232 Msa0894920:three_prime_UTR
AGAGGCATAAAGTTGCATGA+TGG 0.590712 6_1:+81538993 Msa0894920:three_prime_UTR
TGTTAGTGTTGCAAAGTCAG+AGG 0.598215 6_1:+81537786 Msa0894920:exon
TGGAAGTAGTCCTGGCAGGA+AGG 0.600387 6_1:+81535937 Msa0894920:CDS
ATAGTTGGTGCTCCAATGCA+GGG 0.604033 6_1:+81535219 Msa0894920:CDS
TGTTAGTGCTGCAAAGTCAC+AGG 0.610160 6_1:+81535262 Msa0894920:CDS
TGCCTTTAAATACTACATGA+GGG 0.610711 6_1:+81539259 Msa0894920:three_prime_UTR
GCTTGCAAAATGTGATGGCA+AGG 0.615832 6_1:+81539227 Msa0894920:three_prime_UTR
ATCACATCTGCAGATTTGTG+TGG 0.619954 6_1:-81538477 None:intergenic
ACAAATCTGCAGATGTGATG+AGG 0.630753 6_1:+81538481 Msa0894920:three_prime_UTR
GCCAACATCCTCAACCAGCG+TGG 0.639391 6_1:-81535196 None:intergenic
CCAACATCCTCAACCAGCGT+GGG 0.654114 6_1:-81535195 None:intergenic
AGCTGTCAAAGCTACTGATG+AGG 0.663227 6_1:+81536734 Msa0894920:CDS
AATTCTGGGAGACAAAGAAG+AGG 0.676675 6_1:+81538975 Msa0894920:three_prime_UTR
TTTGGGGTTAGAACCCACGC+TGG 0.715063 6_1:+81535182 Msa0894920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAAATTATTTAAT+TGG + chr6_1:81537926-81537945 Msa0894920:intron 0.0%
!! AATAATAATAATAATAATAT+AGG + chr6_1:81538225-81538244 Msa0894920:intron 0.0%
!! AGAATATATTGTTAATATTT+AGG + chr6_1:81535546-81535565 Msa0894920:intron 10.0%
!!! ATTGGTAAAATTATAATTTT+TGG + chr6_1:81537118-81537137 Msa0894920:intron 10.0%
!! GAAATAAAATGAAAAAAATT+TGG - chr6_1:81537286-81537305 None:intergenic 10.0%
!!! TTAATATTTAGGCTTTATTT+GGG + chr6_1:81535557-81535576 Msa0894920:intron 15.0%
!!! ATGAATACTTTTGTTTTTTA+CGG + chr6_1:81535603-81535622 Msa0894920:intron 15.0%
!! AAACATAGTTTGAAAAAAAA+GGG - chr6_1:81535766-81535785 None:intergenic 15.0%
!!! TTTTTTTTATCTGTTTTTTG+TGG + chr6_1:81536040-81536059 Msa0894920:intron 15.0%
!! TATTTCAATTGTTAAATGAA+AGG - chr6_1:81536551-81536570 None:intergenic 15.0%
!! TGAAGCAAAAATTAATAAAA+TGG + chr6_1:81536604-81536623 Msa0894920:intron 15.0%
!!! ATTTAATTTAAATATGTGTG+TGG - chr6_1:81537912-81537931 None:intergenic 15.0%
!!! TGTTGAAGAATTAATTTTAT+TGG + chr6_1:81538292-81538311 Msa0894920:intron 15.0%
!! ATTGGTAAAATTATAATTTC+TGG + chr6_1:81538333-81538352 Msa0894920:intron 15.0%
!!! TATTATTTTTAACTTGCAAT+TGG + chr6_1:81538663-81538682 Msa0894920:intron 15.0%
!! AGTATGAAATGTTAACTTTA+TGG + chr6_1:81535440-81535459 Msa0894920:intron 20.0%
!!! GTTAATATTTAGGCTTTATT+TGG + chr6_1:81535556-81535575 Msa0894920:intron 20.0%
!!! TAATATTTAGGCTTTATTTG+GGG + chr6_1:81535558-81535577 Msa0894920:intron 20.0%
!!! TGAATACTTTTGTTTTTTAC+GGG + chr6_1:81535604-81535623 Msa0894920:intron 20.0%
!! CAAACATAGTTTGAAAAAAA+AGG - chr6_1:81535767-81535786 None:intergenic 20.0%
!!! TTTGTAACATTTTTTAGCTA+TGG - chr6_1:81535793-81535812 None:intergenic 20.0%
!! TAATTCATTTCCAAGTTTAA+TGG - chr6_1:81535985-81536004 None:intergenic 20.0%
!! ATTAAACTTGGAAATGAATT+AGG + chr6_1:81535984-81536003 Msa0894920:CDS 20.0%
!!! TTACAATGATAATGTGTATT+TGG - chr6_1:81536788-81536807 None:intergenic 20.0%
!!! TTATTATTTTTAGCTTGCAA+TGG + chr6_1:81536954-81536973 Msa0894920:intron 20.0%
!!! TATTATTTTTAGCTTGCAAT+GGG + chr6_1:81536955-81536974 Msa0894920:intron 20.0%
!! TCTATTGTAAGAAAAATATG+CGG - chr6_1:81537083-81537102 None:intergenic 20.0%
!!! AAAAATGGGTAATGTTTTTT+CGG + chr6_1:81537163-81537182 Msa0894920:intron 20.0%
!! ATTCAATTGATGAAATGAAA+AGG - chr6_1:81537444-81537463 None:intergenic 20.0%
!! AGAAGTTTGAACAAATAAAT+CGG - chr6_1:81537882-81537901 None:intergenic 20.0%
!! AACATGATTAACCATTATAA+TGG + chr6_1:81538060-81538079 Msa0894920:intron 20.0%
!! TTATATGTAAGGAAAATATG+AGG - chr6_1:81538177-81538196 None:intergenic 20.0%
!! TCATTCAATCATTATATGTA+AGG - chr6_1:81538188-81538207 None:intergenic 20.0%
!! GAAAATATGAAGAGTATAAG+AGG - chr6_1:81535379-81535398 None:intergenic 25.0%
! AAATGTTAACTTTATGGACA+TGG + chr6_1:81535446-81535465 Msa0894920:intron 25.0%
! TTCTTACATGTAGTAGTATT+TGG - chr6_1:81535530-81535549 None:intergenic 25.0%
!!! GAATACTTTTGTTTTTTACG+GGG + chr6_1:81535605-81535624 Msa0894920:intron 25.0%
!!! TTTTTTCTTCCCACATTTTA+AGG + chr6_1:81535686-81535705 Msa0894920:intron 25.0%
! AGAAGACAAAAAGTAAGATA+AGG + chr6_1:81535883-81535902 Msa0894920:intron 25.0%
! AGATAAGGTTATAAAAGTTC+AGG + chr6_1:81535898-81535917 Msa0894920:intron 25.0%
!! CAAAACTCAAAGTATTAACA+AGG - chr6_1:81536230-81536249 None:intergenic 25.0%
!! AGAATTTGAGTTAAAGTCAA+AGG - chr6_1:81536319-81536338 None:intergenic 25.0%
!! AAGTTTTCAAAAGATGGTTA+TGG + chr6_1:81536418-81536437 Msa0894920:intron 25.0%
!! ATAAGATATTAGCTTCAACA+AGG - chr6_1:81536647-81536666 None:intergenic 25.0%
! TTGTTGAAGCTAATATCTTA+TGG + chr6_1:81536646-81536665 Msa0894920:intron 25.0%
! TACTAGGAGAGTTTATTATA+AGG - chr6_1:81537021-81537040 None:intergenic 25.0%
!!! TAATTTTTGGAGACATCTTA+TGG + chr6_1:81537131-81537150 Msa0894920:intron 25.0%
!!! TTATGGATTAGACAGAAAAA+TGG + chr6_1:81537148-81537167 Msa0894920:intron 25.0%
!!! TATGGATTAGACAGAAAAAT+GGG + chr6_1:81537149-81537168 Msa0894920:intron 25.0%
!! AAAAATTTGGATACCCATTT+TGG - chr6_1:81537273-81537292 None:intergenic 25.0%
! TATTCATCGTTATCAAAAGA+AGG - chr6_1:81537327-81537346 None:intergenic 25.0%
!!! TTAATACATTGTTTTGCCTT+TGG + chr6_1:81537577-81537596 Msa0894920:intron 25.0%
! AGAAAATAAACTCAAAGTGA+AGG - chr6_1:81537632-81537651 None:intergenic 25.0%
!!! AAGTTTTCAAATGTTGGTTA+TGG + chr6_1:81538005-81538024 Msa0894920:intron 25.0%
!! TACTATGAGAGTTTTCTATA+AGG - chr6_1:81538115-81538134 None:intergenic 25.0%
!! ACAGTGATAATGTGTATTAT+GGG - chr6_1:81538533-81538552 None:intergenic 25.0%
!! TACAGTGATAATGTGTATTA+TGG - chr6_1:81538534-81538553 None:intergenic 25.0%
! CAATTGTAGAGTATGAAAAA+TGG + chr6_1:81539136-81539155 Msa0894920:three_prime_UTR 25.0%
!!! GTAAAAAGAAGCATAGATTT+TGG + chr6_1:81539205-81539224 Msa0894920:three_prime_UTR 25.0%
!!! TTCATATAGGTTTTATGTTG+TGG + chr6_1:81539397-81539416 Msa0894920:three_prime_UTR 25.0%
!!! TCATATAGGTTTTATGTTGT+GGG + chr6_1:81539398-81539417 Msa0894920:three_prime_UTR 25.0%
TTAACTTTATGGACATGGTA+AGG + chr6_1:81535451-81535470 Msa0894920:intron 30.0%
TAACTTTATGGACATGGTAA+GGG + chr6_1:81535452-81535471 Msa0894920:intron 30.0%
TTACATGTAGTAGTATTTGG+TGG - chr6_1:81535527-81535546 None:intergenic 30.0%
AAATACAGCTATAGCAGAAA+AGG - chr6_1:81535712-81535731 None:intergenic 30.0%
AGACGATTTACAAATAGTCT+TGG - chr6_1:81535822-81535841 None:intergenic 30.0%
! ATCGTCTTTTGTGAAACTTT+TGG + chr6_1:81535835-81535854 Msa0894920:intron 30.0%
! GTTTAATGGCATCATTTTGA+TGG - chr6_1:81535971-81535990 None:intergenic 30.0%
CAAAATGATGCCATTAAACT+TGG + chr6_1:81535972-81535991 Msa0894920:CDS 30.0%
!!! TTATCTGTTTTTTGTGGAAG+AGG + chr6_1:81536046-81536065 Msa0894920:intron 30.0%
TTCAGAAGCAATGTTGTAAA+GGG - chr6_1:81536282-81536301 None:intergenic 30.0%
ATTCAGAAGCAATGTTGTAA+AGG - chr6_1:81536283-81536302 None:intergenic 30.0%
TTTGTTACACAAGGTTTCAT+GGG - chr6_1:81536362-81536381 None:intergenic 30.0%
!! CCATCTTTTGAAAACTTTTG+TGG - chr6_1:81536415-81536434 None:intergenic 30.0%
! CCACAAAAGTTTTCAAAAGA+TGG + chr6_1:81536412-81536431 Msa0894920:intron 30.0%
TTTAATCTACACAAATCTGC+AGG + chr6_1:81536682-81536701 Msa0894920:intron 30.0%
AATGAGAAAGAACACAAAAG+TGG - chr6_1:81536886-81536905 None:intergenic 30.0%
TCTTACAATAGACACATGTT+TGG + chr6_1:81537091-81537110 Msa0894920:intron 30.0%
! TAACGATGAATACCTTTTTC+CGG + chr6_1:81537335-81537354 Msa0894920:intron 30.0%
CAGTTATGAAAAAGGAATAC+CGG - chr6_1:81537357-81537376 None:intergenic 30.0%
CACAAATTCCACATAATCAA+GGG - chr6_1:81537501-81537520 None:intergenic 30.0%
TAAAAGCAATACAAAGCCAA+AGG - chr6_1:81537596-81537615 None:intergenic 30.0%
AGTGTTGTAAAGGGAAAATT+TGG - chr6_1:81537814-81537833 None:intergenic 30.0%
TTTGGAAGTAGTGTTGTAAA+GGG - chr6_1:81537823-81537842 None:intergenic 30.0%
ATTTGGAAGTAGTGTTGTAA+AGG - chr6_1:81537824-81537843 None:intergenic 30.0%
TACAACACTACTTCCAAATT+TGG + chr6_1:81537825-81537844 Msa0894920:intron 30.0%
AAGGAAACAACAACCAAATT+TGG - chr6_1:81537841-81537860 None:intergenic 30.0%
!! TAAATCGGATTTTGAGTCAT+AGG - chr6_1:81537867-81537886 None:intergenic 30.0%
! GAAAACTTTTGAGCATATGT+TGG - chr6_1:81537993-81538012 None:intergenic 30.0%
! GCTCAAAAGTTTTCAAATGT+TGG + chr6_1:81537999-81538018 Msa0894920:intron 30.0%
AGGTATGGCAAAAGAAAAAT+TGG - chr6_1:81538095-81538114 None:intergenic 30.0%
! TGAGAGTTTTCTATAAGGTA+TGG - chr6_1:81538110-81538129 None:intergenic 30.0%
GAAAATATGAGGAATCCAAA+AGG - chr6_1:81538166-81538185 None:intergenic 30.0%
AGACACATGTTTAGTAACAT+TGG + chr6_1:81538315-81538334 Msa0894920:intron 30.0%
!! TTGATTGTTTTGTGCAGAAT+AGG + chr6_1:81538689-81538708 Msa0894920:intron 30.0%
!! TGATTGTTTTGTGCAGAATA+GGG + chr6_1:81538690-81538709 Msa0894920:intron 30.0%
! TAGTACAAAACAGAGGTATA+TGG - chr6_1:81538773-81538792 None:intergenic 30.0%
ACATTCTTAGTACAAAACAG+AGG - chr6_1:81538780-81538799 None:intergenic 30.0%
ACTCTGCATAAGACAATAAT+GGG + chr6_1:81538815-81538834 Msa0894920:intron 30.0%
TTGCCTTTAAATACTACATG+AGG + chr6_1:81539258-81539277 Msa0894920:three_prime_UTR 30.0%
TGCCTTTAAATACTACATGA+GGG + chr6_1:81539259-81539278 Msa0894920:three_prime_UTR 30.0%
TTCCCTCATGTAGTATTTAA+AGG - chr6_1:81539264-81539283 None:intergenic 30.0%
! AGATGTTGATAGGTTCATAT+AGG + chr6_1:81539384-81539403 Msa0894920:three_prime_UTR 30.0%
AAATTCAGGAAACCCATTCA+TGG + chr6_1:81535289-81535308 Msa0894920:intron 35.0%
CATTTCATACTGTTCGTTTC+AGG - chr6_1:81535431-81535450 None:intergenic 35.0%
GGATATGCAAGTGAATAAGA+TGG + chr6_1:81535487-81535506 Msa0894920:intron 35.0%
!! ATTTAGGCTTTATTTGGGGA+AGG + chr6_1:81535562-81535581 Msa0894920:intron 35.0%
!!! ACTTTTGTTTTTTACGGGGA+AGG + chr6_1:81535609-81535628 Msa0894920:intron 35.0%
CTTTATATTCCTGACCTACA+CGG + chr6_1:81535653-81535672 Msa0894920:intron 35.0%
CAGAAAAGGCCTTAAAATGT+GGG - chr6_1:81535698-81535717 None:intergenic 35.0%
AATTCGTTGCAAACTTCAGT+TGG + chr6_1:81536111-81536130 Msa0894920:intron 35.0%
ATTCGTTGCAAACTTCAGTT+GGG + chr6_1:81536112-81536131 Msa0894920:intron 35.0%
TACAACATTGCTTCTGAATC+TGG + chr6_1:81536284-81536303 Msa0894920:intron 35.0%
! GTTTGTTACACAAGGTTTCA+TGG - chr6_1:81536363-81536382 None:intergenic 35.0%
GACTAAGAGAGTCGATTAAA+AGG - chr6_1:81536478-81536497 None:intergenic 35.0%
CTTCATCTCATAGTTTCTCA+AGG - chr6_1:81536713-81536732 None:intergenic 35.0%
CTTGAGAAACTATGAGATGA+AGG + chr6_1:81536711-81536730 Msa0894920:CDS 35.0%
AGAAAGAACACAAAAGTGGA+AGG - chr6_1:81536882-81536901 None:intergenic 35.0%
!!! TTTTAGCTTGCAATGGGTTA+TGG + chr6_1:81536961-81536980 Msa0894920:intron 35.0%
! TTCACGTTCACACTTTTACT+AGG - chr6_1:81537037-81537056 None:intergenic 35.0%
! AGACACATGTTTGGTAACAT+TGG + chr6_1:81537100-81537119 Msa0894920:intron 35.0%
!!! ATTTTGGTTCGATTCTTGCA+TGG - chr6_1:81537257-81537276 None:intergenic 35.0%
ATGCAAGAATCGAACCAAAA+TGG + chr6_1:81537256-81537275 Msa0894920:intron 35.0%
TGCAAGAATCGAACCAAAAT+GGG + chr6_1:81537257-81537276 Msa0894920:intron 35.0%
GAAAAAGGAATACCGGAAAA+AGG - chr6_1:81537350-81537369 None:intergenic 35.0%
CTATGCAGCAGTTATGAAAA+AGG - chr6_1:81537365-81537384 None:intergenic 35.0%
CTTAATCATTATGATGCCCT+TGG - chr6_1:81537409-81537428 None:intergenic 35.0%
ACGTTATTCCCTTGATTATG+TGG + chr6_1:81537490-81537509 Msa0894920:intron 35.0%
CCACAAATTCCACATAATCA+AGG - chr6_1:81537502-81537521 None:intergenic 35.0%
CCTTGATTATGTGGAATTTG+TGG + chr6_1:81537499-81537518 Msa0894920:intron 35.0%
! TTTCTTTTGATCAGGTGGTT+AGG + chr6_1:81537696-81537715 Msa0894920:intron 35.0%
!! GATTTTGAGTCATAGGTCAA+AGG - chr6_1:81537860-81537879 None:intergenic 35.0%
! GAATTCCATTGTATGCCTTT+TGG + chr6_1:81538148-81538167 Msa0894920:intron 35.0%
! TAATTTCTGGAGACGTCTTA+TGG + chr6_1:81538346-81538365 Msa0894920:intron 35.0%
GACTCTGCATAAGACAATAA+TGG + chr6_1:81538814-81538833 Msa0894920:intron 35.0%
CAACTAAGAGCTTATCTCAT+TGG - chr6_1:81539087-81539106 None:intergenic 35.0%
!!! TTTTGGCTTGCAAAATGTGA+TGG + chr6_1:81539222-81539241 Msa0894920:three_prime_UTR 35.0%
AAGTCACAGGTGACAAATTC+AGG + chr6_1:81535275-81535294 Msa0894920:intron 40.0%
TACAGAAGGTCCATCTAGAT+GGG - chr6_1:81535346-81535365 None:intergenic 40.0%
TTACAGAAGGTCCATCTAGA+TGG - chr6_1:81535347-81535366 None:intergenic 40.0%
GAGGAAAGAGAACTTACAGA+AGG - chr6_1:81535360-81535379 None:intergenic 40.0%
GACATGGTAAGGGTTAGAAA+AGG + chr6_1:81535462-81535481 Msa0894920:intron 40.0%
TGGTAAGGGTTAGAAAAGGA+TGG + chr6_1:81535466-81535485 Msa0894920:intron 40.0%
!! GCTTTATTTGGGGAAGGTAA+GGG + chr6_1:81535568-81535587 Msa0894920:intron 40.0%
GGGAAGGTTGAACTTGATAT+GGG + chr6_1:81535625-81535644 Msa0894920:intron 40.0%
GGAAGGTTGAACTTGATATG+GGG + chr6_1:81535626-81535645 Msa0894920:intron 40.0%
AAAACAAAGAGCTACCGTGT+AGG - chr6_1:81535670-81535689 None:intergenic 40.0%
GCAGAAAAGGCCTTAAAATG+TGG - chr6_1:81535699-81535718 None:intergenic 40.0%
GCCATATCTGACATACAATC+TGG + chr6_1:81535740-81535759 Msa0894920:intron 40.0%
GCCAGATTGTATGTCAGATA+TGG - chr6_1:81535744-81535763 None:intergenic 40.0%
! GAAACTTTTGGCATGTTTGC+AGG + chr6_1:81535847-81535866 Msa0894920:intron 40.0%
!!! TCTGTTTTTTGTGGAAGAGG+TGG + chr6_1:81536049-81536068 Msa0894920:intron 40.0%
! TTTTGTGGAAGAGGTGGAAA+TGG + chr6_1:81536055-81536074 Msa0894920:intron 40.0%
TTCGTTGCAAACTTCAGTTG+GGG + chr6_1:81536113-81536132 Msa0894920:intron 40.0%
! TTGATGCATCGTTTTGCCTT+TGG + chr6_1:81536166-81536185 Msa0894920:intron 40.0%
GAGCAGAAACACAAATCCAA+AGG - chr6_1:81536185-81536204 None:intergenic 40.0%
!! AAGGGATAATTTGCTACCTC+TGG - chr6_1:81536264-81536283 None:intergenic 40.0%
! GTGAGTGTGTTTGTTACACA+AGG - chr6_1:81536371-81536390 None:intergenic 40.0%
AGAGAGTCGATTAAAAGGCA+TGG - chr6_1:81536473-81536492 None:intergenic 40.0%
GAATTCCATTGCATGCTTCT+AGG + chr6_1:81536511-81536530 Msa0894920:intron 40.0%
GAATTCCATTGTATGCCTCT+TGG + chr6_1:81537054-81537073 Msa0894920:intron 40.0%
AAAAATATGCGGAGTCCAAG+AGG - chr6_1:81537072-81537091 None:intergenic 40.0%
! CTGCTGCATAGATCTTTTTG+TGG + chr6_1:81537374-81537393 Msa0894920:intron 40.0%
! TTTTGTGGCAGCTAAATCCA+AGG + chr6_1:81537389-81537408 Msa0894920:intron 40.0%
TTTGTGGCAGCTAAATCCAA+GGG + chr6_1:81537390-81537409 Msa0894920:intron 40.0%
TCTCGCAACAGTATAAGAGA+CGG + chr6_1:81537669-81537688 Msa0894920:intron 40.0%
!! CTCAAGCTTGTTGCATATGT+TGG + chr6_1:81537720-81537739 Msa0894920:CDS 40.0%
TGTTAGTGTTGCAAAGTCAG+AGG + chr6_1:81537786-81537805 Msa0894920:exon 40.0%
GGCTTGCAAGTCCATTATAA+TGG - chr6_1:81538074-81538093 None:intergenic 40.0%
! GGAATCCAAAAGGCATACAA+TGG - chr6_1:81538156-81538175 None:intergenic 40.0%
TTTGCGGAATTGTCTGATGA+AGG + chr6_1:81538452-81538471 Msa0894920:three_prime_UTR 40.0%
ATCACATCTGCAGATTTGTG+TGG - chr6_1:81538480-81538499 None:intergenic 40.0%
ACAAATCTGCAGATGTGATG+AGG + chr6_1:81538481-81538500 Msa0894920:three_prime_UTR 40.0%
GTTCCTCCTTCAAGAATTCT+GGG + chr6_1:81538961-81538980 Msa0894920:intron 40.0%
TTGTCTCCCAGAATTCTTGA+AGG - chr6_1:81538970-81538989 None:intergenic 40.0%
! AATTCTGGGAGACAAAGAAG+AGG + chr6_1:81538975-81538994 Msa0894920:three_prime_UTR 40.0%
! AGAGGCATAAAGTTGCATGA+TGG + chr6_1:81538993-81539012 Msa0894920:three_prime_UTR 40.0%
GTTGAGGATGTTGGCATAGT+TGG + chr6_1:81535204-81535223 Msa0894920:CDS 45.0%
! ATAGTTGGTGCTCCAATGCA+GGG + chr6_1:81535219-81535238 Msa0894920:CDS 45.0%
! GAGTGTACTTTTCCCTGCAT+TGG - chr6_1:81535234-81535253 None:intergenic 45.0%
TGTTAGTGCTGCAAAGTCAC+AGG + chr6_1:81535262-81535281 Msa0894920:CDS 45.0%
AGGAAACCCATTCATGGAGT+GGG + chr6_1:81535295-81535314 Msa0894920:intron 45.0%
ACCATTTCTCCCCATCTAGA+TGG + chr6_1:81535333-81535352 Msa0894920:intron 45.0%
TCCATCTAGATGGGGAGAAA+TGG - chr6_1:81535337-81535356 None:intergenic 45.0%
ACAGAAGGTCCATCTAGATG+GGG - chr6_1:81535345-81535364 None:intergenic 45.0%
!! GGCTTTATTTGGGGAAGGTA+AGG + chr6_1:81535567-81535586 Msa0894920:intron 45.0%
GGGGAAGGTTGAACTTGATA+TGG + chr6_1:81535624-81535643 Msa0894920:intron 45.0%
GGTTGAACTTGATATGGGGA+AGG + chr6_1:81535630-81535649 Msa0894920:intron 45.0%
TGTCTTTGTGGAAGTAGTCC+TGG + chr6_1:81535929-81535948 Msa0894920:CDS 45.0%
TCGTTGCAAACTTCAGTTGG+GGG + chr6_1:81536114-81536133 Msa0894920:intron 45.0%
! TTTTGCTAGTGCAAAGCCAG+AGG + chr6_1:81536245-81536264 Msa0894920:intron 45.0%
! CAAGTCCTAGAAGCATGCAA+TGG - chr6_1:81536519-81536538 None:intergenic 45.0%
AGCTGTCAAAGCTACTGATG+AGG + chr6_1:81536734-81536753 Msa0894920:CDS 45.0%
!!! ACGGCTGGTTTCTTTTGATC+AGG + chr6_1:81537688-81537707 Msa0894920:intron 45.0%
!! GCTGGTTTCTTTTGATCAGG+TGG + chr6_1:81537691-81537710 Msa0894920:intron 45.0%
TTCTTGTCGACATACCTGCT+TGG + chr6_1:81538414-81538433 Msa0894920:three_prime_UTR 45.0%
! AGACAATTCCGCAAAGCGTT+TGG - chr6_1:81538447-81538466 None:intergenic 45.0%
GGTTCCTCCTTCAAGAATTC+TGG + chr6_1:81538960-81538979 Msa0894920:intron 45.0%
TCTCCCAGAATTCTTGAAGG+AGG - chr6_1:81538967-81538986 None:intergenic 45.0%
GCTTGCAAAATGTGATGGCA+AGG + chr6_1:81539227-81539246 Msa0894920:three_prime_UTR 45.0%
CAAAATGTGATGGCAAGGCA+AGG + chr6_1:81539232-81539251 Msa0894920:three_prime_UTR 45.0%
AAGCTCTTCCTTTCTCTCCA+AGG - chr6_1:81539296-81539315 None:intergenic 45.0%
TTGGAGAGAAAGGAAGAGCT+TGG + chr6_1:81539295-81539314 Msa0894920:three_prime_UTR 45.0%
AGAAAGAGCCTTGGAGAGAA+AGG + chr6_1:81539330-81539349 Msa0894920:three_prime_UTR 45.0%
CTGCAGATGCAGATGTTGAT+AGG + chr6_1:81539374-81539393 Msa0894920:three_prime_UTR 45.0%
!!! TAAGATTTTTTTTTAAAAAA+GGG - chr6_1:81536146-81536165 None:intergenic 5.0%
!!! ATAAGATTTTTTTTTAAAAA+AGG - chr6_1:81536147-81536166 None:intergenic 5.0%
!! TAATTATAAATATAATTGTT+AGG + chr6_1:81538847-81538866 Msa0894920:intron 5.0%
!! AATTATAAATATAATTGTTA+GGG + chr6_1:81538848-81538867 Msa0894920:intron 5.0%
! CATAGTTGGTGCTCCAATGC+AGG + chr6_1:81535218-81535237 Msa0894920:CDS 50.0%
CAGGAAACCCATTCATGGAG+TGG + chr6_1:81535294-81535313 Msa0894920:intron 50.0%
GGAAACCCATTCATGGAGTG+GGG + chr6_1:81535296-81535315 Msa0894920:intron 50.0%
TCATGCCCCACTCCATGAAT+GGG - chr6_1:81535304-81535323 None:intergenic 50.0%
ATCATGCCCCACTCCATGAA+TGG - chr6_1:81535305-81535324 None:intergenic 50.0%
AAAGAGCTACCGTGTAGGTC+AGG - chr6_1:81535665-81535684 None:intergenic 50.0%
TTTGTGGAAGTAGTCCTGGC+AGG + chr6_1:81535933-81535952 Msa0894920:CDS 50.0%
CGTTGCAAACTTCAGTTGGG+GGG + chr6_1:81536115-81536134 Msa0894920:intron 50.0%
! GGAGTCCAAGAGGCATACAA+TGG - chr6_1:81537062-81537081 None:intergenic 50.0%
GCAACAGTATAAGAGACGGC+TGG + chr6_1:81537673-81537692 Msa0894920:intron 50.0%
GCTGCTATCCAAACGCTTTG+CGG + chr6_1:81538436-81538455 Msa0894920:three_prime_UTR 50.0%
GCCTTCATACTGCTTTCTGC+TGG + chr6_1:81538934-81538953 Msa0894920:intron 50.0%
GCCAGCAGAAAGCAGTATGA+AGG - chr6_1:81538938-81538957 None:intergenic 50.0%
CGAAAGAGCCTTGGAGAGAA+AGG + chr6_1:81539285-81539304 Msa0894920:three_prime_UTR 50.0%
GTGAGAGAGAGAAAGAGCCT+TGG + chr6_1:81539321-81539340 Msa0894920:three_prime_UTR 50.0%
AGAATTTGCAGTGCAGCGCA+GGG - chr6_1:81539356-81539375 None:intergenic 50.0%
! TTTGGGGTTAGAACCCACGC+TGG + chr6_1:81535182-81535201 Msa0894920:CDS 55.0%
! GTTAGAACCCACGCTGGTTG+AGG + chr6_1:81535188-81535207 Msa0894920:CDS 55.0%
CCAACATCCTCAACCAGCGT+GGG - chr6_1:81535198-81535217 None:intergenic 55.0%
! CAGGAGAGCACGTGTCTTTG+TGG + chr6_1:81535917-81535936 Msa0894920:intron 55.0%
TGGAAGTAGTCCTGGCAGGA+AGG + chr6_1:81535937-81535956 Msa0894920:CDS 55.0%
CGTTTGGATAGCAGCCAAGC+AGG - chr6_1:81538431-81538450 None:intergenic 55.0%
CATACTGCTTTCTGCTGGCG+TGG + chr6_1:81538939-81538958 Msa0894920:intron 55.0%
TGAGGGAAGCGAAAGAGCCT+TGG + chr6_1:81539276-81539295 Msa0894920:three_prime_UTR 55.0%
CAGAATTTGCAGTGCAGCGC+AGG - chr6_1:81539357-81539376 None:intergenic 55.0%
! CCCACGCTGGTTGAGGATGT+TGG + chr6_1:81535195-81535214 Msa0894920:CDS 60.0%
GCCAACATCCTCAACCAGCG+TGG - chr6_1:81535199-81535218 None:intergenic 60.0%
GGCTACTCTTCCTTCCTGCC+AGG - chr6_1:81535950-81535969 None:intergenic 60.0%
GCGCAGGGCCTTTCTCTCCA+AGG - chr6_1:81539341-81539360 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 81535180 81539443 81535180 ID=Msa0894920;Name=Msa0894920
chr6_1 mRNA 81535180 81539443 81535180 ID=Msa0894920-mRNA-1;Parent=Msa0894920;Name=Msa0894920-mRNA-1;_AED=0.28;_eAED=0.35;_QI=0|0.8|0.66|1|0.6|0.33|6|628|123
chr6_1 exon 81535180 81535283 81535180 ID=Msa0894920-mRNA-1:exon:13752;Parent=Msa0894920-mRNA-1
chr6_1 exon 81535920 81536005 81535920 ID=Msa0894920-mRNA-1:exon:13753;Parent=Msa0894920-mRNA-1
chr6_1 exon 81536704 81536803 81536704 ID=Msa0894920-mRNA-1:exon:13754;Parent=Msa0894920-mRNA-1
chr6_1 exon 81537710 81537807 81537710 ID=Msa0894920-mRNA-1:exon:13755;Parent=Msa0894920-mRNA-1
chr6_1 exon 81538408 81538550 81538408 ID=Msa0894920-mRNA-1:exon:13756;Parent=Msa0894920-mRNA-1
chr6_1 exon 81538975 81539443 81538975 ID=Msa0894920-mRNA-1:exon:13757;Parent=Msa0894920-mRNA-1
chr6_1 CDS 81535180 81535283 81535180 ID=Msa0894920-mRNA-1:cds;Parent=Msa0894920-mRNA-1
chr6_1 CDS 81535920 81536005 81535920 ID=Msa0894920-mRNA-1:cds;Parent=Msa0894920-mRNA-1
chr6_1 CDS 81536704 81536803 81536704 ID=Msa0894920-mRNA-1:cds;Parent=Msa0894920-mRNA-1
chr6_1 CDS 81537710 81537791 81537710 ID=Msa0894920-mRNA-1:cds;Parent=Msa0894920-mRNA-1
chr6_1 three_prime_UTR 81537792 81537807 81537792 ID=Msa0894920-mRNA-1:three_prime_utr;Parent=Msa0894920-mRNA-1
chr6_1 three_prime_UTR 81538408 81538550 81538408 ID=Msa0894920-mRNA-1:three_prime_utr;Parent=Msa0894920-mRNA-1
chr6_1 three_prime_UTR 81538975 81539443 81538975 ID=Msa0894920-mRNA-1:three_prime_utr;Parent=Msa0894920-mRNA-1
Gene Sequence

>Msa0894920

TGTTTGGGGTTAGAACCCACGCTGGTTGAGGATGTTGGCATAGTTGGTGCTCCAATGCAGGGAAAAGTACACTCTTCAGTGTTGTTAGTGCTGCAAAGTCACAGGAGAGCACGTGTCTTTGTGGAAGTAGTCCTGGCAGGAAGGAAGAGTAGCCATCAAAATGATGCCATTAAACTTGGAAATGAATTAGGTGACACCTTGAGAAACTATGAGATGAAGGAGCTGTCAAAGCTACTGATGAGGACTTGTTTACATGCTTGCTTGCTGCTGTCCAAATACACATTATCATTGTGGTTAGGACTCAAGCTTGTTGCATATGTTGGTATAGTTGATGCTTCAAATGAAGAAAAGCACAATTTTGAGCTTTGTTAG
Protein sequence

>Msa0894920

CLGLEPTLVEDVGIVGAPMQGKVHSSVLLVLQSHRRARVFVEVVLAGRKSSHQNDAIKLGNELGDTLRNYEMKELSKLLMRTCLHACLLLSKYTLSLWLGLKLVAYVGIVDASNEEKHNFELC*