Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016580 | Msa0886740 | 0.800766 | 1.235271e-48 | -8.615850e-47 |
| Msa0811080 | Msa0886740 | 0.800629 | 1.317801e-48 | -8.615850e-47 |
| Msa0886740 | Msa0886750 | 0.862378 | 5.288473e-64 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGCTTTCAACTCTTTAC+CGG | 0.359875 | 6_1:-46773569 | Msa0886740:CDS |
| CGGAGAAACGTGCTTTAGCC+TGG | 0.403389 | 6_1:-46773549 | Msa0886740:CDS |
| CAGGCTAAAGCACGTTTCTC+CGG | 0.520831 | 6_1:+46773550 | None:intergenic |
| GATCTTCAAGAGGAGGGTCA+TGG | 0.556611 | 6_1:-46773151 | Msa0886740:CDS |
| CTTACCAGCATACTCAATCC+AGG | 0.562842 | 6_1:+46773531 | None:intergenic |
| TTAGCCTGGATTGAGTATGC+TGG | 0.581572 | 6_1:-46773535 | Msa0886740:CDS |
| TGAGTTTGATCTTCAAGAGG+AGG | 0.586038 | 6_1:-46773158 | Msa0886740:CDS |
| ATGTGAGTTTGATCTTCAAG+AGG | 0.591258 | 6_1:-46773161 | Msa0886740:CDS |
| TCTTGAAGATCAAACTCACA+TGG | 0.605591 | 6_1:+46773163 | None:intergenic |
| AATTAAGGATGGTAATGGTG+TGG | 0.643410 | 6_1:-46774180 | None:intergenic |
| GAGTTTGATCTTCAAGAGGA+GGG | 0.644093 | 6_1:-46773157 | Msa0886740:CDS |
| GCTGCTATTGAATTATCACA+AGG | 0.693449 | 6_1:-46773127 | Msa0886740:CDS |
| CTGCTATTGAATTATCACAA+GGG | 0.696629 | 6_1:-46773126 | Msa0886740:CDS |
| AATGGTGTGGAAGACAGTGA+AGG | 0.700992 | 6_1:-46774167 | Msa0886740:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCTAACTTATAATAGAT+AGG | - | chr6_1:46773642-46773661 | Msa0886740:intron | 15.0% |
| !!! | TGACTATTTTTGTTTTTATT+AGG | - | chr6_1:46773669-46773688 | Msa0886740:intron | 15.0% |
| !! | ATAATTACAATTCTTCAATA+TGG | - | chr6_1:46773804-46773823 | Msa0886740:intron | 15.0% |
| !! | TTATCTAACTTATAATAGAT+AGG | - | chr6_1:46773642-46773661 | Msa0886740:intron | 15.0% |
| !!! | TGACTATTTTTGTTTTTATT+AGG | - | chr6_1:46773669-46773688 | Msa0886740:intron | 15.0% |
| !! | ATAATTACAATTCTTCAATA+TGG | - | chr6_1:46773804-46773823 | Msa0886740:intron | 15.0% |
| !!! | TACATTTTATGTTCATGATT+TGG | - | chr6_1:46773236-46773255 | Msa0886740:intron | 20.0% |
| !!! | TTTTGTATATGATTAAGACT+TGG | - | chr6_1:46773308-46773327 | Msa0886740:intron | 20.0% |
| !! | AGTTTAGACTAACATTATTA+AGG | - | chr6_1:46773369-46773388 | Msa0886740:intron | 20.0% |
| !!! | ATATACTATGATTTTCAGAT+TGG | - | chr6_1:46773563-46773582 | Msa0886740:CDS | 20.0% |
| !! | GAATTGTAATTATCAAGATA+TGG | + | chr6_1:46773797-46773816 | None:intergenic | 20.0% |
| !!! | ATTATGTTCTTTAAGACATA+AGG | - | chr6_1:46773873-46773892 | Msa0886740:intron | 20.0% |
| !! | ATGTACAGTATAAATAACTT+TGG | - | chr6_1:46774058-46774077 | Msa0886740:intron | 20.0% |
| !!! | TACATTTTATGTTCATGATT+TGG | - | chr6_1:46773236-46773255 | Msa0886740:intron | 20.0% |
| !!! | TTTTGTATATGATTAAGACT+TGG | - | chr6_1:46773308-46773327 | Msa0886740:intron | 20.0% |
| !! | AGTTTAGACTAACATTATTA+AGG | - | chr6_1:46773369-46773388 | Msa0886740:intron | 20.0% |
| !!! | ATATACTATGATTTTCAGAT+TGG | - | chr6_1:46773563-46773582 | Msa0886740:CDS | 20.0% |
| !! | GAATTGTAATTATCAAGATA+TGG | + | chr6_1:46773797-46773816 | None:intergenic | 20.0% |
| !!! | ATTATGTTCTTTAAGACATA+AGG | - | chr6_1:46773873-46773892 | Msa0886740:intron | 20.0% |
| !! | ATGTACAGTATAAATAACTT+TGG | - | chr6_1:46774058-46774077 | Msa0886740:intron | 20.0% |
| ! | AAGACAAAAGTCATTATCAT+TGG | + | chr6_1:46773182-46773201 | None:intergenic | 25.0% |
| !!! | ATATCAATGATAGCTTGTTT+TGG | - | chr6_1:46773333-46773352 | Msa0886740:intron | 25.0% |
| ! | TCATAGTATATAAACCATCA+TGG | + | chr6_1:46773554-46773573 | None:intergenic | 25.0% |
| !! | GTTTATGTTGTCCAATTTTA+AGG | - | chr6_1:46773771-46773790 | Msa0886740:intron | 25.0% |
| ! | AAACATTCAAAACTAACACA+CGG | + | chr6_1:46773920-46773939 | None:intergenic | 25.0% |
| !!! | TGTTGACAATAGATTTTGAT+AGG | - | chr6_1:46774023-46774042 | Msa0886740:intron | 25.0% |
| !!! | GTTGACAATAGATTTTGATA+GGG | - | chr6_1:46774024-46774043 | Msa0886740:intron | 25.0% |
| ! | AAGACAAAAGTCATTATCAT+TGG | + | chr6_1:46773182-46773201 | None:intergenic | 25.0% |
| !!! | ATATCAATGATAGCTTGTTT+TGG | - | chr6_1:46773333-46773352 | Msa0886740:intron | 25.0% |
| ! | TCATAGTATATAAACCATCA+TGG | + | chr6_1:46773554-46773573 | None:intergenic | 25.0% |
| !! | GTTTATGTTGTCCAATTTTA+AGG | - | chr6_1:46773771-46773790 | Msa0886740:intron | 25.0% |
| ! | AAACATTCAAAACTAACACA+CGG | + | chr6_1:46773920-46773939 | None:intergenic | 25.0% |
| !!! | TGTTGACAATAGATTTTGAT+AGG | - | chr6_1:46774023-46774042 | Msa0886740:intron | 25.0% |
| !!! | GTTGACAATAGATTTTGATA+GGG | - | chr6_1:46774024-46774043 | Msa0886740:intron | 25.0% |
| ! | CTTGGCATTTTATCTTGAAT+TGG | - | chr6_1:46773207-46773226 | Msa0886740:intron | 30.0% |
| AAACCATTACTCGTATTTAG+TGG | + | chr6_1:46773271-46773290 | None:intergenic | 30.0% | |
| ! | GAGTAATGGTTTCTTTTGAT+TGG | - | chr6_1:46773279-46773298 | Msa0886740:intron | 30.0% |
| ! | ATCATCTTGATCTCTTTTCA+TGG | + | chr6_1:46773440-46773459 | None:intergenic | 30.0% |
| TTCAGATTACTCTCAAAGAA+TGG | - | chr6_1:46773527-46773546 | Msa0886740:intron | 30.0% | |
| TTATGCTTTCAACTCTTTAC+CGG | - | chr6_1:46773711-46773730 | Msa0886740:intron | 30.0% | |
| ! | TCAAGATATGGCCTTAAAAT+TGG | + | chr6_1:46773785-46773804 | None:intergenic | 30.0% |
| AATGTTTGTGATTCCTACTA+TGG | - | chr6_1:46773933-46773952 | Msa0886740:intron | 30.0% | |
| ! | TCCTACTATGGATTATCATA+TGG | - | chr6_1:46773945-46773964 | Msa0886740:intron | 30.0% |
| TCCATATGATAATCCATAGT+AGG | + | chr6_1:46773949-46773968 | None:intergenic | 30.0% | |
| !! | ATAACAAGAAGCATCATTGT+AGG | + | chr6_1:46773996-46774015 | None:intergenic | 30.0% |
| CTGCTATTGAATTATCACAA+GGG | - | chr6_1:46774154-46774173 | Msa0886740:intron | 30.0% | |
| ! | CTTGGCATTTTATCTTGAAT+TGG | - | chr6_1:46773207-46773226 | Msa0886740:intron | 30.0% |
| AAACCATTACTCGTATTTAG+TGG | + | chr6_1:46773271-46773290 | None:intergenic | 30.0% | |
| ! | GAGTAATGGTTTCTTTTGAT+TGG | - | chr6_1:46773279-46773298 | Msa0886740:intron | 30.0% |
| ! | ATCATCTTGATCTCTTTTCA+TGG | + | chr6_1:46773440-46773459 | None:intergenic | 30.0% |
| TTCAGATTACTCTCAAAGAA+TGG | - | chr6_1:46773527-46773546 | Msa0886740:intron | 30.0% | |
| TTATGCTTTCAACTCTTTAC+CGG | - | chr6_1:46773711-46773730 | Msa0886740:intron | 30.0% | |
| ! | TCAAGATATGGCCTTAAAAT+TGG | + | chr6_1:46773785-46773804 | None:intergenic | 30.0% |
| AATGTTTGTGATTCCTACTA+TGG | - | chr6_1:46773933-46773952 | Msa0886740:intron | 30.0% | |
| ! | TCCTACTATGGATTATCATA+TGG | - | chr6_1:46773945-46773964 | Msa0886740:intron | 30.0% |
| TCCATATGATAATCCATAGT+AGG | + | chr6_1:46773949-46773968 | None:intergenic | 30.0% | |
| !! | ATAACAAGAAGCATCATTGT+AGG | + | chr6_1:46773996-46774015 | None:intergenic | 30.0% |
| CTGCTATTGAATTATCACAA+GGG | - | chr6_1:46774154-46774173 | Msa0886740:intron | 30.0% | |
| ! | CATTATCATTGGTTGCTAAG+AGG | + | chr6_1:46773171-46773190 | None:intergenic | 35.0% |
| ! | TGACTTTTGTCTTCATCTCT+TGG | - | chr6_1:46773189-46773208 | Msa0886740:intron | 35.0% |
| CAACCACTAAATACGAGTAA+TGG | - | chr6_1:46773265-46773284 | Msa0886740:intron | 35.0% | |
| ! | CTATGATTTTCAGATTGGAG+AGG | - | chr6_1:46773568-46773587 | Msa0886740:CDS | 35.0% |
| GCTATTATATATTGTCCCAC+AGG | - | chr6_1:46774085-46774104 | Msa0886740:intron | 35.0% | |
| TCTTGAAGATCAAACTCACA+TGG | + | chr6_1:46774120-46774139 | None:intergenic | 35.0% | |
| ATGTGAGTTTGATCTTCAAG+AGG | - | chr6_1:46774119-46774138 | Msa0886740:intron | 35.0% | |
| GCTGCTATTGAATTATCACA+AGG | - | chr6_1:46774153-46774172 | Msa0886740:intron | 35.0% | |
| ! | CATTATCATTGGTTGCTAAG+AGG | + | chr6_1:46773171-46773190 | None:intergenic | 35.0% |
| ! | TGACTTTTGTCTTCATCTCT+TGG | - | chr6_1:46773189-46773208 | Msa0886740:intron | 35.0% |
| CAACCACTAAATACGAGTAA+TGG | - | chr6_1:46773265-46773284 | Msa0886740:intron | 35.0% | |
| ! | CTATGATTTTCAGATTGGAG+AGG | - | chr6_1:46773568-46773587 | Msa0886740:CDS | 35.0% |
| GCTATTATATATTGTCCCAC+AGG | - | chr6_1:46774085-46774104 | Msa0886740:intron | 35.0% | |
| TCTTGAAGATCAAACTCACA+TGG | + | chr6_1:46774120-46774139 | None:intergenic | 35.0% | |
| ATGTGAGTTTGATCTTCAAG+AGG | - | chr6_1:46774119-46774138 | Msa0886740:intron | 35.0% | |
| GCTGCTATTGAATTATCACA+AGG | - | chr6_1:46774153-46774172 | Msa0886740:intron | 35.0% | |
| TCTCAAAGAATGGTCCATGA+TGG | - | chr6_1:46773537-46773556 | Msa0886740:CDS | 40.0% | |
| TGAGTTTGATCTTCAAGAGG+AGG | - | chr6_1:46774122-46774141 | Msa0886740:intron | 40.0% | |
| GAGTTTGATCTTCAAGAGGA+GGG | - | chr6_1:46774123-46774142 | Msa0886740:intron | 40.0% | |
| TCTCAAAGAATGGTCCATGA+TGG | - | chr6_1:46773537-46773556 | Msa0886740:CDS | 40.0% | |
| TGAGTTTGATCTTCAAGAGG+AGG | - | chr6_1:46774122-46774141 | Msa0886740:intron | 40.0% | |
| GAGTTTGATCTTCAAGAGGA+GGG | - | chr6_1:46774123-46774142 | Msa0886740:intron | 40.0% | |
| GTGGAAGACAGTGAAGGTAA+TGG | - | chr6_1:46773119-46773138 | Msa0886740:CDS | 45.0% | |
| TTAGCCTGGATTGAGTATGC+TGG | - | chr6_1:46773745-46773764 | Msa0886740:intron | 45.0% | |
| CTTACCAGCATACTCAATCC+AGG | + | chr6_1:46773752-46773771 | None:intergenic | 45.0% | |
| ! | ACATGGAAGTGCATACCTGT+GGG | + | chr6_1:46774103-46774122 | None:intergenic | 45.0% |
| GTGGAAGACAGTGAAGGTAA+TGG | - | chr6_1:46773119-46773138 | Msa0886740:CDS | 45.0% | |
| TTAGCCTGGATTGAGTATGC+TGG | - | chr6_1:46773745-46773764 | Msa0886740:intron | 45.0% | |
| CTTACCAGCATACTCAATCC+AGG | + | chr6_1:46773752-46773771 | None:intergenic | 45.0% | |
| ! | ACATGGAAGTGCATACCTGT+GGG | + | chr6_1:46774103-46774122 | None:intergenic | 45.0% |
| CAGGCTAAAGCACGTTTCTC+CGG | + | chr6_1:46773733-46773752 | None:intergenic | 50.0% | |
| ! | CACATGGAAGTGCATACCTG+TGG | + | chr6_1:46774104-46774123 | None:intergenic | 50.0% |
| GATCTTCAAGAGGAGGGTCA+TGG | - | chr6_1:46774129-46774148 | Msa0886740:intron | 50.0% | |
| CAGGCTAAAGCACGTTTCTC+CGG | + | chr6_1:46773733-46773752 | None:intergenic | 50.0% | |
| ! | CACATGGAAGTGCATACCTG+TGG | + | chr6_1:46774104-46774123 | None:intergenic | 50.0% |
| GATCTTCAAGAGGAGGGTCA+TGG | - | chr6_1:46774129-46774148 | Msa0886740:intron | 50.0% | |
| CGGAGAAACGTGCTTTAGCC+TGG | - | chr6_1:46773731-46773750 | Msa0886740:intron | 55.0% | |
| CGGAGAAACGTGCTTTAGCC+TGG | - | chr6_1:46773731-46773750 | Msa0886740:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 46773114 | 46774188 | 46773114 | ID=Msa0886740;Name=Msa0886740 |
| chr6_1 | mRNA | 46773114 | 46774188 | 46773114 | ID=Msa0886740-mRNA-1;Parent=Msa0886740;Name=Msa0886740-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|0.66|1|1|3|0|60 |
| chr6_1 | exon | 46774168 | 46774188 | 46774168 | ID=Msa0886740-mRNA-1:exon:9853;Parent=Msa0886740-mRNA-1 |
| chr6_1 | exon | 46773532 | 46773611 | 46773532 | ID=Msa0886740-mRNA-1:exon:9852;Parent=Msa0886740-mRNA-1 |
| chr6_1 | exon | 46773114 | 46773195 | 46773114 | ID=Msa0886740-mRNA-1:exon:9851;Parent=Msa0886740-mRNA-1 |
| chr6_1 | CDS | 46774168 | 46774188 | 46774168 | ID=Msa0886740-mRNA-1:cds;Parent=Msa0886740-mRNA-1 |
| chr6_1 | CDS | 46773532 | 46773611 | 46773532 | ID=Msa0886740-mRNA-1:cds;Parent=Msa0886740-mRNA-1 |
| chr6_1 | CDS | 46773114 | 46773195 | 46773114 | ID=Msa0886740-mRNA-1:cds;Parent=Msa0886740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
