AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0016580


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTCTAGTTCATAATCTTT+TGG 0.250279 1_1:-27494348 None:intergenic
AGTTGAGATAAAGAGGAAAA+TGG 0.370037 1_1:+27494224 Msa0016580:CDS
ACCAAAAGATTATGAACTAG+AGG 0.481301 1_1:+27494347 Msa0016580:CDS
ATACATCAAATATGAATCCA+AGG 0.538148 1_1:+27494322 Msa0016580:CDS
GATGCAGAGCTGTAGAAGGC+TGG 0.573955 1_1:+27494249 Msa0016580:intron
CCAAAAGATTATGAACTAGA+GGG 0.607622 1_1:+27494348 Msa0016580:CDS
TACATCAAATATGAATCCAA+GGG 0.613150 1_1:+27494323 Msa0016580:CDS
TGATTTAGAAATACTCTCGA+AGG 0.636930 1_1:+27494299 Msa0016580:CDS
AGGCTGGTAATGATAGCGAA+AGG 0.640851 1_1:+27494265 Msa0016580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATACATCAAATATGAATCCA+AGG + chr1_1:27494322-27494341 Msa0016580:CDS 25.0%
! TACATCAAATATGAATCCAA+GGG + chr1_1:27494323-27494342 Msa0016580:CDS 25.0%
GTGAAAAAGTTGAGATAAAG+AGG + chr1_1:27494217-27494236 Msa0016580:CDS 30.0%
AGTTGAGATAAAGAGGAAAA+TGG + chr1_1:27494224-27494243 Msa0016580:CDS 30.0%
TGATTTAGAAATACTCTCGA+AGG + chr1_1:27494299-27494318 Msa0016580:CDS 30.0%
ACCAAAAGATTATGAACTAG+AGG + chr1_1:27494347-27494366 Msa0016580:CDS 30.0%
CCAAAAGATTATGAACTAGA+GGG + chr1_1:27494348-27494367 Msa0016580:CDS 30.0%
!!! TCATAATCTTTTGGTTCCCT+TGG - chr1_1:27494342-27494361 None:intergenic 35.0%
!! CCCTCTAGTTCATAATCTTT+TGG - chr1_1:27494351-27494370 None:intergenic 35.0%
! AGGCTGGTAATGATAGCGAA+AGG + chr1_1:27494265-27494284 Msa0016580:CDS 45.0%
! GGTTGATGCAGAGCTGTAGA+AGG + chr1_1:27494245-27494264 Msa0016580:intron 50.0%
GATGCAGAGCTGTAGAAGGC+TGG + chr1_1:27494249-27494268 Msa0016580:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 27494180 27494386 27494180 ID=Msa0016580;Name=Msa0016580
chr1_1 mRNA 27494180 27494386 27494180 ID=Msa0016580-mRNA-1;Parent=Msa0016580;Name=Msa0016580-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|0|0|2|0|62
chr1_1 exon 27494180 27494245 27494180 ID=Msa0016580-mRNA-1:exon:9285;Parent=Msa0016580-mRNA-1
chr1_1 exon 27494264 27494386 27494264 ID=Msa0016580-mRNA-1:exon:9286;Parent=Msa0016580-mRNA-1
chr1_1 CDS 27494180 27494245 27494180 ID=Msa0016580-mRNA-1:cds;Parent=Msa0016580-mRNA-1
chr1_1 CDS 27494264 27494386 27494264 ID=Msa0016580-mRNA-1:cds;Parent=Msa0016580-mRNA-1
Gene Sequence

>Msa0016580

ATGTCAAGGTTTGCACATGATTTTGCACGTATTGAAAGTGAAAAAGTTGAGATAAAGAGGAAAATGAAGGCTGGTAATGATAGCGAAAGGATGAAGATCAATGATTTAGAAATACTCTCGAAGGATACATCAAATATGAATCCAAGGGAACCAAAAGATTATGAACTAGAGGGATATTATAATTTTTAG

Protein sequence

>Msa0016580

MSRFAHDFARIESEKVEIKRKMKAGNDSERMKINDLEILSKDTSNMNPREPKDYELEGYYNF*