AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0811080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 24 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAGTATCAATCTTATAT+AGG 0.366162 5_3:+40195210 Msa0811080:five_prime_UTR
CCACTTGATTCCCTTGCGTT+GGG 0.409880 5_3:-40195721 None:intergenic
TCAGTCTCAGTTTGCACGGC+TGG 0.418620 5_3:+40195246 Msa0811080:five_prime_UTR
CCCACTTGATTCCCTTGCGT+TGG 0.431316 5_3:-40195722 None:intergenic
AGGAAGCAATAAATTTAAGC+TGG 0.504728 5_3:+40195182 Msa0811080:five_prime_UTR
CGAGATACAACAATCTCATC+GGG 0.510114 5_3:+40195819 Msa0811080:CDS
GAGATCGGAAAGTTCTTCTC+AGG 0.511097 5_3:+40195669 Msa0811080:CDS
CAGGAAAGTAAAACACAAGA+TGG 0.533616 5_3:+40195688 Msa0811080:CDS
GTTCCTCTGTTCCCAACGCA+AGG 0.538012 5_3:+40195710 Msa0811080:CDS
AGCAGCAACAAAAGTAGTGA+AGG 0.542395 5_3:+40195156 Msa0811080:five_prime_UTR
ATTGTGTTAGGATGGGAGAT+CGG 0.543831 5_3:+40195654 Msa0811080:intron
TCGAGATACAACAATCTCAT+CGG 0.561426 5_3:+40195818 Msa0811080:CDS
AACAAAAGTAGTGAAGGAAG+AGG 0.570751 5_3:+40195162 Msa0811080:five_prime_UTR
CAGCCGCGAGTGTTAAAAGT+GGG 0.578565 5_3:+40195749 Msa0811080:CDS
CCCAACGCAAGGGAATCAAG+TGG 0.588631 5_3:+40195721 Msa0811080:CDS
TTCCCTTGCGTTGGGAACAG+AGG 0.594506 5_3:-40195713 None:intergenic
CCAACGCAAGGGAATCAAGT+GGG 0.597329 5_3:+40195722 Msa0811080:CDS
GCAGTCAGTCTCAGTTTGCA+CGG 0.601159 5_3:+40195242 Msa0811080:five_prime_UTR
TTCCTCTGTTCCCAACGCAA+GGG 0.617747 5_3:+40195711 Msa0811080:CDS
CAACGCAAGGGAATCAAGTG+GGG 0.622276 5_3:+40195723 Msa0811080:CDS
GCCGCGAGTGTTAAAAGTGG+GGG 0.633082 5_3:+40195751 Msa0811080:CDS
TCAGCCGCGAGTGTTAAAAG+TGG 0.638737 5_3:+40195748 Msa0811080:CDS
AGCCGCGAGTGTTAAAAGTG+GGG 0.663832 5_3:+40195750 Msa0811080:CDS
AACGCAAGGGAATCAAGTGG+GGG 0.669401 5_3:+40195724 Msa0811080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAGTATCAATCTTATAT+AGG + chr5_3:40195210-40195229 Msa0811080:five_prime_UTR 20.0%
!! TATACAATAGAATACTTAAG+TGG - chr5_3:40195494-40195513 None:intergenic 20.0%
! TATTACATCTGTATAGTTAC+TGG + chr5_3:40195327-40195346 Msa0811080:intron 25.0%
! GAAACATGTATAGGTATAAT+TGG - chr5_3:40195582-40195601 None:intergenic 25.0%
AGGAAGCAATAAATTTAAGC+TGG + chr5_3:40195182-40195201 Msa0811080:five_prime_UTR 30.0%
! ATATCATTAGTTTCCTAGCT+GGG - chr5_3:40195470-40195489 None:intergenic 30.0%
! AATATCATTAGTTTCCTAGC+TGG - chr5_3:40195471-40195490 None:intergenic 30.0%
! TCAATCGTGTCAAATTTTCT+TGG + chr5_3:40195532-40195551 Msa0811080:intron 30.0%
! CAATCGTGTCAAATTTTCTT+GGG + chr5_3:40195533-40195552 Msa0811080:intron 30.0%
GCATAGTATGAAACATGTAT+AGG - chr5_3:40195591-40195610 None:intergenic 30.0%
!! CGCATGATTTTAACATTTAC+TGG - chr5_3:40195629-40195648 None:intergenic 30.0%
! AAGAACTTGTGTTTTACTTG+CGG - chr5_3:40195782-40195801 None:intergenic 30.0%
AACAAAAGTAGTGAAGGAAG+AGG + chr5_3:40195162-40195181 Msa0811080:five_prime_UTR 35.0%
TACATCTGTATAGTTACTGG+TGG + chr5_3:40195330-40195349 Msa0811080:intron 35.0%
CTACCTAAGCTTCATCTATA+AGG + chr5_3:40195442-40195461 Msa0811080:intron 35.0%
CAGGAAAGTAAAACACAAGA+TGG + chr5_3:40195688-40195707 Msa0811080:CDS 35.0%
TCGAGATACAACAATCTCAT+CGG + chr5_3:40195818-40195837 Msa0811080:CDS 35.0%
AGCAGCAACAAAAGTAGTGA+AGG + chr5_3:40195156-40195175 Msa0811080:five_prime_UTR 40.0%
! TCTAAGATGCGCACCTAATT+TGG + chr5_3:40195355-40195374 Msa0811080:intron 40.0%
CTAAGATGCGCACCTAATTT+GGG + chr5_3:40195356-40195375 Msa0811080:intron 40.0%
TAAGATGCGCACCTAATTTG+GGG + chr5_3:40195357-40195376 Msa0811080:intron 40.0%
AAGTTTCCAGTGGCCAATAA+GGG - chr5_3:40195396-40195415 None:intergenic 40.0%
GTGCCTTATAGATGAAGCTT+AGG - chr5_3:40195448-40195467 None:intergenic 40.0%
TCATGCGCTTGAATTGTGTT+AGG + chr5_3:40195642-40195661 Msa0811080:intron 40.0%
CGCTTGAATTGTGTTAGGAT+GGG + chr5_3:40195647-40195666 Msa0811080:intron 40.0%
ATTGTGTTAGGATGGGAGAT+CGG + chr5_3:40195654-40195673 Msa0811080:intron 40.0%
CGAGATACAACAATCTCATC+GGG + chr5_3:40195819-40195838 Msa0811080:CDS 40.0%
! TGTACGCTTGCTACCCTTAT+TGG + chr5_3:40195380-40195399 Msa0811080:intron 45.0%
CAAGTTTCCAGTGGCCAATA+AGG - chr5_3:40195397-40195416 None:intergenic 45.0%
TTCCTCTGACAAGTTTCCAG+TGG - chr5_3:40195406-40195425 None:intergenic 45.0%
GCGCTTGAATTGTGTTAGGA+TGG + chr5_3:40195646-40195665 Msa0811080:intron 45.0%
GAGATCGGAAAGTTCTTCTC+AGG + chr5_3:40195669-40195688 Msa0811080:CDS 45.0%
GCAGTCAGTCTCAGTTTGCA+CGG + chr5_3:40195242-40195261 Msa0811080:five_prime_UTR 50.0%
GCAAGCGTACACCCCAAATT+AGG - chr5_3:40195371-40195390 None:intergenic 50.0%
TTGCTACCCTTATTGGCCAC+TGG + chr5_3:40195387-40195406 Msa0811080:intron 50.0%
! CATCTATAAGGCACCCAGCT+AGG + chr5_3:40195454-40195473 Msa0811080:intron 50.0%
TTCCTCTGTTCCCAACGCAA+GGG + chr5_3:40195711-40195730 Msa0811080:CDS 50.0%
CCACTTGATTCCCTTGCGTT+GGG - chr5_3:40195724-40195743 None:intergenic 50.0%
CCAACGCAAGGGAATCAAGT+GGG + chr5_3:40195722-40195741 Msa0811080:CDS 50.0%
CAACGCAAGGGAATCAAGTG+GGG + chr5_3:40195723-40195742 Msa0811080:CDS 50.0%
AACGCAAGGGAATCAAGTGG+GGG + chr5_3:40195724-40195743 Msa0811080:CDS 50.0%
TCAGCCGCGAGTGTTAAAAG+TGG + chr5_3:40195748-40195767 Msa0811080:CDS 50.0%
CAGCCGCGAGTGTTAAAAGT+GGG + chr5_3:40195749-40195768 Msa0811080:CDS 50.0%
AGCCGCGAGTGTTAAAAGTG+GGG + chr5_3:40195750-40195769 Msa0811080:CDS 50.0%
!! TCCCCCACTTTTAACACTCG+CGG - chr5_3:40195755-40195774 None:intergenic 50.0%
TCAGTCTCAGTTTGCACGGC+TGG + chr5_3:40195246-40195265 Msa0811080:five_prime_UTR 55.0%
GGCCACTGGAAACTTGTCAG+AGG + chr5_3:40195401-40195420 Msa0811080:intron 55.0%
GTTCCTCTGTTCCCAACGCA+AGG + chr5_3:40195710-40195729 Msa0811080:CDS 55.0%
TTCCCTTGCGTTGGGAACAG+AGG - chr5_3:40195716-40195735 None:intergenic 55.0%
CCCAACGCAAGGGAATCAAG+TGG + chr5_3:40195721-40195740 Msa0811080:CDS 55.0%
CCCACTTGATTCCCTTGCGT+TGG - chr5_3:40195725-40195744 None:intergenic 55.0%
GCCGCGAGTGTTAAAAGTGG+GGG + chr5_3:40195751-40195770 Msa0811080:CDS 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 40195150 40195844 40195150 ID=Msa0811080;Name=Msa0811080
chr5_3 mRNA 40195150 40195844 40195150 ID=Msa0811080-mRNA-1;Parent=Msa0811080;Name=Msa0811080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=139|1|0.5|1|0|0.5|2|0|64
chr5_3 exon 40195150 40195299 40195150 ID=Msa0811080-mRNA-1:exon:12278;Parent=Msa0811080-mRNA-1
chr5_3 exon 40195664 40195844 40195664 ID=Msa0811080-mRNA-1:exon:12279;Parent=Msa0811080-mRNA-1
chr5_3 five_prime_UTR 40195150 40195288 40195150 ID=Msa0811080-mRNA-1:five_prime_utr;Parent=Msa0811080-mRNA-1
chr5_3 CDS 40195289 40195299 40195289 ID=Msa0811080-mRNA-1:cds;Parent=Msa0811080-mRNA-1
chr5_3 CDS 40195664 40195844 40195664 ID=Msa0811080-mRNA-1:cds;Parent=Msa0811080-mRNA-1
Gene Sequence

>Msa0811080

ATGCTAATCATGATGGGAGATCGGAAAGTTCTTCTCAGGAAAGTAAAACACAAGATGGTTCCTCTGTTCCCAACGCAAGGGAATCAAGTGGGGGATCAGCCGCGAGTGTTAAAAGTGGGGGATCAGCCGCAAGTAAAACACAAGTTCTTCAGTGCTGTTAAAAGCTCGAGATACAACAATCTCATCGGGTAT

Protein sequence

>Msa0811080

MLIMMGDRKVLLRKVKHKMVPLFPTQGNQVGDQPRVLKVGDQPQVKHKFFSAVKSSRYNNLIGY