Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015750 | Msa0886750 | 0.808644 | 2.798915e-50 | -8.615850e-47 |
| Msa0016570 | Msa0886750 | 0.835088 | 2.056768e-56 | -8.615850e-47 |
| Msa0016580 | Msa0886750 | 0.824503 | 7.793650e-54 | -8.615850e-47 |
| Msa0110270 | Msa0886750 | 0.813818 | 2.110777e-51 | -8.615850e-47 |
| Msa0636170 | Msa0886750 | 0.817195 | 3.740256e-52 | -8.615850e-47 |
| Msa0678930 | Msa0886750 | 0.806664 | 7.371125e-50 | -8.615850e-47 |
| Msa0741570 | Msa0886750 | 0.803463 | 3.444241e-49 | -8.615850e-47 |
| Msa0745060 | Msa0886750 | 0.827230 | 1.754278e-54 | -8.615850e-47 |
| Msa0767720 | Msa0886750 | 0.820492 | 6.666445e-53 | -8.615850e-47 |
| Msa0789250 | Msa0886750 | 0.809242 | 2.084783e-50 | -8.615850e-47 |
| Msa0811080 | Msa0886750 | 0.857394 | 1.682180e-62 | -8.615850e-47 |
| Msa0825860 | Msa0886750 | 0.815292 | 9.961980e-52 | -8.615850e-47 |
| Msa0142220 | Msa0886750 | 0.828143 | 1.059083e-54 | -8.615850e-47 |
| Msa0456200 | Msa0886750 | 0.803027 | 4.239735e-49 | -8.615850e-47 |
| Msa0326200 | Msa0886750 | 0.812110 | 4.999401e-51 | -8.615850e-47 |
| Msa0353190 | Msa0886750 | 0.817319 | 3.508024e-52 | -8.615850e-47 |
| Msa0885290 | Msa0886750 | 0.814832 | 1.260414e-51 | -8.615850e-47 |
| Msa0886740 | Msa0886750 | 0.862378 | 5.288473e-64 | -8.615850e-47 |
| Msa0886750 | Msa0991230 | 0.807754 | 4.331758e-50 | -8.615850e-47 |
| Msa0886750 | Msa1024240 | 0.818405 | 1.994192e-52 | -8.615850e-47 |
| Msa0886750 | Msa1024370 | 0.830363 | 3.063907e-55 | -8.615850e-47 |
| Msa0886750 | Msa1092990 | 0.819385 | 1.194139e-52 | -8.615850e-47 |
| Msa0886750 | Msa1146110 | 0.829625 | 4.636647e-55 | -8.615850e-47 |
| Msa0886750 | Msa1148020 | 0.812964 | 3.252700e-51 | -8.615850e-47 |
| Msa0886750 | Msa1160670 | 0.817392 | 3.376376e-52 | -8.615850e-47 |
| Msa0886750 | Msa1160690 | 0.809413 | 1.915454e-50 | -8.615850e-47 |
| Msa0886750 | Msa1187940 | 0.832644 | 8.403544e-56 | -8.615850e-47 |
| Msa0886750 | Msa1269670 | 0.810299 | 1.235297e-50 | -8.615850e-47 |
| Msa0886750 | Msa1433120 | 0.800013 | 1.758592e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATGCTTTCAACTCTTTAC+CGG | 0.359875 | 6_1:-46778671 | Msa0886750:CDS |
| CGGAGAAACGTGCTTTAGCC+TGG | 0.403389 | 6_1:-46778651 | Msa0886750:CDS |
| CAGGCTAAAGCACGTTTCTC+CGG | 0.520831 | 6_1:+46778652 | None:intergenic |
| GATCTTCAAGAGGAGGGTCA+TGG | 0.556611 | 6_1:-46778253 | Msa0886750:CDS |
| CTTACCAGCATACTCAATCC+AGG | 0.562842 | 6_1:+46778633 | None:intergenic |
| TTAGCCTGGATTGAGTATGC+TGG | 0.581572 | 6_1:-46778637 | Msa0886750:CDS |
| TGAGTTTGATCTTCAAGAGG+AGG | 0.586038 | 6_1:-46778260 | Msa0886750:CDS |
| ATGTGAGTTTGATCTTCAAG+AGG | 0.591258 | 6_1:-46778263 | Msa0886750:CDS |
| TCTTGAAGATCAAACTCACA+TGG | 0.605591 | 6_1:+46778265 | None:intergenic |
| AATTAAGGATGGTAATGGTG+TGG | 0.643410 | 6_1:-46779281 | None:intergenic |
| GAGTTTGATCTTCAAGAGGA+GGG | 0.644093 | 6_1:-46778259 | Msa0886750:CDS |
| GCTGCTATTGAATTATCACA+AGG | 0.693449 | 6_1:-46778229 | Msa0886750:CDS |
| CTGCTATTGAATTATCACAA+GGG | 0.696629 | 6_1:-46778228 | Msa0886750:CDS |
| AATGGTGTGGAAGACAGTGA+AGG | 0.700992 | 6_1:-46779268 | Msa0886750:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCTAACTTATAATAGAT+AGG | - | chr6_1:46778743-46778762 | Msa0886750:intron | 15.0% |
| !!! | TGACTATTTTTGTTTTTATT+AGG | - | chr6_1:46778770-46778789 | Msa0886750:intron | 15.0% |
| !! | ATAATTACAATTCTTCAATA+TGG | - | chr6_1:46778905-46778924 | Msa0886750:intron | 15.0% |
| !! | TTATCTAACTTATAATAGAT+AGG | - | chr6_1:46778743-46778762 | Msa0886750:intron | 15.0% |
| !!! | TGACTATTTTTGTTTTTATT+AGG | - | chr6_1:46778770-46778789 | Msa0886750:intron | 15.0% |
| !! | ATAATTACAATTCTTCAATA+TGG | - | chr6_1:46778905-46778924 | Msa0886750:intron | 15.0% |
| !!! | TACATTTTATGTTCATGATT+TGG | - | chr6_1:46778338-46778357 | Msa0886750:intron | 20.0% |
| !!! | TTTTGTATATGATTAAGACT+TGG | - | chr6_1:46778410-46778429 | Msa0886750:intron | 20.0% |
| !! | AGTTTAGACTAACATTATTA+AGG | - | chr6_1:46778471-46778490 | Msa0886750:intron | 20.0% |
| !!! | ATATACTATGATTTTCAGAT+TGG | - | chr6_1:46778664-46778683 | Msa0886750:CDS | 20.0% |
| !! | GAATTGTAATTATCAAGATA+TGG | + | chr6_1:46778898-46778917 | None:intergenic | 20.0% |
| !!! | ATTATGTTCTTTAAGACATA+AGG | - | chr6_1:46778974-46778993 | Msa0886750:intron | 20.0% |
| !! | ATGTACAGTATAAATAACTT+TGG | - | chr6_1:46779159-46779178 | Msa0886750:intron | 20.0% |
| !!! | TACATTTTATGTTCATGATT+TGG | - | chr6_1:46778338-46778357 | Msa0886750:intron | 20.0% |
| !!! | TTTTGTATATGATTAAGACT+TGG | - | chr6_1:46778410-46778429 | Msa0886750:intron | 20.0% |
| !! | AGTTTAGACTAACATTATTA+AGG | - | chr6_1:46778471-46778490 | Msa0886750:intron | 20.0% |
| !!! | ATATACTATGATTTTCAGAT+TGG | - | chr6_1:46778664-46778683 | Msa0886750:CDS | 20.0% |
| !! | GAATTGTAATTATCAAGATA+TGG | + | chr6_1:46778898-46778917 | None:intergenic | 20.0% |
| !!! | ATTATGTTCTTTAAGACATA+AGG | - | chr6_1:46778974-46778993 | Msa0886750:intron | 20.0% |
| !! | ATGTACAGTATAAATAACTT+TGG | - | chr6_1:46779159-46779178 | Msa0886750:intron | 20.0% |
| ! | AAGACAAAAGTCATTATCAT+TGG | + | chr6_1:46778284-46778303 | None:intergenic | 25.0% |
| !!! | ATATCAATGATAGCTTGTTT+TGG | - | chr6_1:46778435-46778454 | Msa0886750:intron | 25.0% |
| ! | TCATAGTATATAAACCATCA+TGG | + | chr6_1:46778655-46778674 | None:intergenic | 25.0% |
| !! | GTTTATGTTGTCCAATTTTA+AGG | - | chr6_1:46778872-46778891 | Msa0886750:intron | 25.0% |
| ! | AAACATTCAAAACTAACACA+CGG | + | chr6_1:46779021-46779040 | None:intergenic | 25.0% |
| !!! | TGTTGACAATAGATTTTGAT+AGG | - | chr6_1:46779124-46779143 | Msa0886750:intron | 25.0% |
| !!! | GTTGACAATAGATTTTGATA+GGG | - | chr6_1:46779125-46779144 | Msa0886750:intron | 25.0% |
| ! | AAGACAAAAGTCATTATCAT+TGG | + | chr6_1:46778284-46778303 | None:intergenic | 25.0% |
| !!! | ATATCAATGATAGCTTGTTT+TGG | - | chr6_1:46778435-46778454 | Msa0886750:intron | 25.0% |
| ! | TCATAGTATATAAACCATCA+TGG | + | chr6_1:46778655-46778674 | None:intergenic | 25.0% |
| !! | GTTTATGTTGTCCAATTTTA+AGG | - | chr6_1:46778872-46778891 | Msa0886750:intron | 25.0% |
| ! | AAACATTCAAAACTAACACA+CGG | + | chr6_1:46779021-46779040 | None:intergenic | 25.0% |
| !!! | TGTTGACAATAGATTTTGAT+AGG | - | chr6_1:46779124-46779143 | Msa0886750:intron | 25.0% |
| !!! | GTTGACAATAGATTTTGATA+GGG | - | chr6_1:46779125-46779144 | Msa0886750:intron | 25.0% |
| ! | CTTGGCATTTTATCTTGAAT+TGG | - | chr6_1:46778309-46778328 | Msa0886750:intron | 30.0% |
| AAACCATTACTCGTATTTAG+TGG | + | chr6_1:46778373-46778392 | None:intergenic | 30.0% | |
| ! | GAGTAATGGTTTCTTTTGAT+TGG | - | chr6_1:46778381-46778400 | Msa0886750:intron | 30.0% |
| ! | ATCATCTTGATCTCTTTTCA+TGG | + | chr6_1:46778541-46778560 | None:intergenic | 30.0% |
| TTCAGATTACTCTCAAAGAA+TGG | - | chr6_1:46778628-46778647 | Msa0886750:intron | 30.0% | |
| TTATGCTTTCAACTCTTTAC+CGG | - | chr6_1:46778812-46778831 | Msa0886750:intron | 30.0% | |
| ! | TCAAGATATGGCCTTAAAAT+TGG | + | chr6_1:46778886-46778905 | None:intergenic | 30.0% |
| AATGTTTGTGATTCCTACTA+TGG | - | chr6_1:46779034-46779053 | Msa0886750:intron | 30.0% | |
| ! | TCCTACTATGGATTATCATA+TGG | - | chr6_1:46779046-46779065 | Msa0886750:intron | 30.0% |
| TCCATATGATAATCCATAGT+AGG | + | chr6_1:46779050-46779069 | None:intergenic | 30.0% | |
| !! | ATAACAAGAAGCATCATTGT+AGG | + | chr6_1:46779097-46779116 | None:intergenic | 30.0% |
| CTGCTATTGAATTATCACAA+GGG | - | chr6_1:46779255-46779274 | Msa0886750:intron | 30.0% | |
| ! | CTTGGCATTTTATCTTGAAT+TGG | - | chr6_1:46778309-46778328 | Msa0886750:intron | 30.0% |
| AAACCATTACTCGTATTTAG+TGG | + | chr6_1:46778373-46778392 | None:intergenic | 30.0% | |
| ! | GAGTAATGGTTTCTTTTGAT+TGG | - | chr6_1:46778381-46778400 | Msa0886750:intron | 30.0% |
| ! | ATCATCTTGATCTCTTTTCA+TGG | + | chr6_1:46778541-46778560 | None:intergenic | 30.0% |
| TTCAGATTACTCTCAAAGAA+TGG | - | chr6_1:46778628-46778647 | Msa0886750:intron | 30.0% | |
| TTATGCTTTCAACTCTTTAC+CGG | - | chr6_1:46778812-46778831 | Msa0886750:intron | 30.0% | |
| ! | TCAAGATATGGCCTTAAAAT+TGG | + | chr6_1:46778886-46778905 | None:intergenic | 30.0% |
| AATGTTTGTGATTCCTACTA+TGG | - | chr6_1:46779034-46779053 | Msa0886750:intron | 30.0% | |
| ! | TCCTACTATGGATTATCATA+TGG | - | chr6_1:46779046-46779065 | Msa0886750:intron | 30.0% |
| TCCATATGATAATCCATAGT+AGG | + | chr6_1:46779050-46779069 | None:intergenic | 30.0% | |
| !! | ATAACAAGAAGCATCATTGT+AGG | + | chr6_1:46779097-46779116 | None:intergenic | 30.0% |
| CTGCTATTGAATTATCACAA+GGG | - | chr6_1:46779255-46779274 | Msa0886750:intron | 30.0% | |
| ! | CATTATCATTGGTTGCTAAG+AGG | + | chr6_1:46778273-46778292 | None:intergenic | 35.0% |
| ! | TGACTTTTGTCTTCATCTCT+TGG | - | chr6_1:46778291-46778310 | Msa0886750:intron | 35.0% |
| CAACCACTAAATACGAGTAA+TGG | - | chr6_1:46778367-46778386 | Msa0886750:intron | 35.0% | |
| ! | CTATGATTTTCAGATTGGAG+AGG | - | chr6_1:46778669-46778688 | Msa0886750:CDS | 35.0% |
| GCTATTATATATTGTCCCAC+AGG | - | chr6_1:46779186-46779205 | Msa0886750:intron | 35.0% | |
| TCTTGAAGATCAAACTCACA+TGG | + | chr6_1:46779221-46779240 | None:intergenic | 35.0% | |
| ATGTGAGTTTGATCTTCAAG+AGG | - | chr6_1:46779220-46779239 | Msa0886750:intron | 35.0% | |
| GCTGCTATTGAATTATCACA+AGG | - | chr6_1:46779254-46779273 | Msa0886750:intron | 35.0% | |
| ! | CATTATCATTGGTTGCTAAG+AGG | + | chr6_1:46778273-46778292 | None:intergenic | 35.0% |
| ! | TGACTTTTGTCTTCATCTCT+TGG | - | chr6_1:46778291-46778310 | Msa0886750:intron | 35.0% |
| CAACCACTAAATACGAGTAA+TGG | - | chr6_1:46778367-46778386 | Msa0886750:intron | 35.0% | |
| ! | CTATGATTTTCAGATTGGAG+AGG | - | chr6_1:46778669-46778688 | Msa0886750:CDS | 35.0% |
| GCTATTATATATTGTCCCAC+AGG | - | chr6_1:46779186-46779205 | Msa0886750:intron | 35.0% | |
| TCTTGAAGATCAAACTCACA+TGG | + | chr6_1:46779221-46779240 | None:intergenic | 35.0% | |
| ATGTGAGTTTGATCTTCAAG+AGG | - | chr6_1:46779220-46779239 | Msa0886750:intron | 35.0% | |
| GCTGCTATTGAATTATCACA+AGG | - | chr6_1:46779254-46779273 | Msa0886750:intron | 35.0% | |
| TCTCAAAGAATGGTCCATGA+TGG | - | chr6_1:46778638-46778657 | Msa0886750:CDS | 40.0% | |
| TGAGTTTGATCTTCAAGAGG+AGG | - | chr6_1:46779223-46779242 | Msa0886750:intron | 40.0% | |
| GAGTTTGATCTTCAAGAGGA+GGG | - | chr6_1:46779224-46779243 | Msa0886750:intron | 40.0% | |
| TCTCAAAGAATGGTCCATGA+TGG | - | chr6_1:46778638-46778657 | Msa0886750:CDS | 40.0% | |
| TGAGTTTGATCTTCAAGAGG+AGG | - | chr6_1:46779223-46779242 | Msa0886750:intron | 40.0% | |
| GAGTTTGATCTTCAAGAGGA+GGG | - | chr6_1:46779224-46779243 | Msa0886750:intron | 40.0% | |
| GTGGAAGACAGTGAAGGTAA+TGG | - | chr6_1:46778221-46778240 | Msa0886750:CDS | 45.0% | |
| TTAGCCTGGATTGAGTATGC+TGG | - | chr6_1:46778846-46778865 | Msa0886750:intron | 45.0% | |
| CTTACCAGCATACTCAATCC+AGG | + | chr6_1:46778853-46778872 | None:intergenic | 45.0% | |
| ! | ACATGGAAGTGCATACCTGT+GGG | + | chr6_1:46779204-46779223 | None:intergenic | 45.0% |
| GTGGAAGACAGTGAAGGTAA+TGG | - | chr6_1:46778221-46778240 | Msa0886750:CDS | 45.0% | |
| TTAGCCTGGATTGAGTATGC+TGG | - | chr6_1:46778846-46778865 | Msa0886750:intron | 45.0% | |
| CTTACCAGCATACTCAATCC+AGG | + | chr6_1:46778853-46778872 | None:intergenic | 45.0% | |
| ! | ACATGGAAGTGCATACCTGT+GGG | + | chr6_1:46779204-46779223 | None:intergenic | 45.0% |
| CAGGCTAAAGCACGTTTCTC+CGG | + | chr6_1:46778834-46778853 | None:intergenic | 50.0% | |
| ! | CACATGGAAGTGCATACCTG+TGG | + | chr6_1:46779205-46779224 | None:intergenic | 50.0% |
| GATCTTCAAGAGGAGGGTCA+TGG | - | chr6_1:46779230-46779249 | Msa0886750:intron | 50.0% | |
| CAGGCTAAAGCACGTTTCTC+CGG | + | chr6_1:46778834-46778853 | None:intergenic | 50.0% | |
| ! | CACATGGAAGTGCATACCTG+TGG | + | chr6_1:46779205-46779224 | None:intergenic | 50.0% |
| GATCTTCAAGAGGAGGGTCA+TGG | - | chr6_1:46779230-46779249 | Msa0886750:intron | 50.0% | |
| CGGAGAAACGTGCTTTAGCC+TGG | - | chr6_1:46778832-46778851 | Msa0886750:intron | 55.0% | |
| CGGAGAAACGTGCTTTAGCC+TGG | - | chr6_1:46778832-46778851 | Msa0886750:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 46778216 | 46779289 | 46778216 | ID=Msa0886750;Name=Msa0886750 |
| chr6_1 | mRNA | 46778216 | 46779289 | 46778216 | ID=Msa0886750-mRNA-1;Parent=Msa0886750;Name=Msa0886750-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|0.66|1|1|3|0|60 |
| chr6_1 | exon | 46779269 | 46779289 | 46779269 | ID=Msa0886750-mRNA-1:exon:9850;Parent=Msa0886750-mRNA-1 |
| chr6_1 | exon | 46778634 | 46778713 | 46778634 | ID=Msa0886750-mRNA-1:exon:9849;Parent=Msa0886750-mRNA-1 |
| chr6_1 | exon | 46778216 | 46778297 | 46778216 | ID=Msa0886750-mRNA-1:exon:9848;Parent=Msa0886750-mRNA-1 |
| chr6_1 | CDS | 46779269 | 46779289 | 46779269 | ID=Msa0886750-mRNA-1:cds;Parent=Msa0886750-mRNA-1 |
| chr6_1 | CDS | 46778634 | 46778713 | 46778634 | ID=Msa0886750-mRNA-1:cds;Parent=Msa0886750-mRNA-1 |
| chr6_1 | CDS | 46778216 | 46778297 | 46778216 | ID=Msa0886750-mRNA-1:cds;Parent=Msa0886750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
