Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0657730 | Msa0893140 | 0.833336 | 5.655176e-56 | -8.615850e-47 |
| Msa0828940 | Msa0893140 | 0.845814 | 3.211335e-59 | -8.615850e-47 |
| Msa0893140 | Msa1153410 | 0.815406 | 9.395344e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAGGAAGAAGGGAAAT+TGG | 0.330889 | 6_1:-75981063 | Msa0893140:CDS |
| ACTACGATATGAAGGCGTTT+TGG | 0.352736 | 6_1:-75980204 | Msa0893140:intron |
| AGGAGCGTTGATTGATGATC+TGG | 0.403255 | 6_1:-75980122 | Msa0893140:CDS |
| AATGGAGGGAGAGGAGAAGA+AGG | 0.408209 | 6_1:-75981096 | Msa0893140:CDS |
| AGAAGGGAAATTGGAGAAGA+AGG | 0.422017 | 6_1:-75981054 | Msa0893140:CDS |
| AATTTCCCTTCTTCCTCCAT+CGG | 0.440960 | 6_1:+75981065 | None:intergenic |
| AAGAAGCCGATGGAGGAAGA+AGG | 0.492336 | 6_1:-75981071 | Msa0893140:CDS |
| GAACGAGAGAATGGAGGGAG+AGG | 0.515280 | 6_1:-75981105 | Msa0893140:exon |
| AAACAGAACGAGAGAATGGA+GGG | 0.517664 | 6_1:-75981110 | Msa0893140:exon |
| GAAGAAGGAAAAGAAGCCGA+TGG | 0.563967 | 6_1:-75981081 | Msa0893140:CDS |
| AGAAGCCGATGGAGGAAGAA+GGG | 0.579655 | 6_1:-75981070 | Msa0893140:CDS |
| TTCATCGTACTACGATGTCT+AGG | 0.580982 | 6_1:-75980142 | Msa0893140:CDS |
| AAAACAGAACGAGAGAATGG+AGG | 0.592760 | 6_1:-75981111 | Msa0893140:exon |
| GAAGGAAAAGAAGCCGATGG+AGG | 0.645079 | 6_1:-75981078 | Msa0893140:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATTAAGAAATTGAAATT+GGG | + | chr6_1:75980290-75980309 | None:intergenic | 15.0% |
| !! | AATTTCAATTTCTTAATGAA+GGG | - | chr6_1:75980290-75980309 | Msa0893140:intron | 15.0% |
| !! | TTCATTAAGAAATTGAAATT+GGG | + | chr6_1:75980290-75980309 | None:intergenic | 15.0% |
| !! | AATTTCAATTTCTTAATGAA+GGG | - | chr6_1:75980290-75980309 | Msa0893140:intron | 15.0% |
| !! | TCATTAAGAAATTGAAATTG+GGG | + | chr6_1:75980289-75980308 | None:intergenic | 20.0% |
| !! | CTTCATTAAGAAATTGAAAT+TGG | + | chr6_1:75980291-75980310 | None:intergenic | 20.0% |
| !! | CAATTTCAATTTCTTAATGA+AGG | - | chr6_1:75980289-75980308 | Msa0893140:intron | 20.0% |
| !! | AATTAAGCTTTCTTTGAATA+AGG | + | chr6_1:75980343-75980362 | None:intergenic | 20.0% |
| !!! | TGTAATTGTTTTATGATGAA+TGG | - | chr6_1:75980821-75980840 | Msa0893140:intron | 20.0% |
| !! | TCATTAAGAAATTGAAATTG+GGG | + | chr6_1:75980289-75980308 | None:intergenic | 20.0% |
| !! | CTTCATTAAGAAATTGAAAT+TGG | + | chr6_1:75980291-75980310 | None:intergenic | 20.0% |
| !! | CAATTTCAATTTCTTAATGA+AGG | - | chr6_1:75980289-75980308 | Msa0893140:intron | 20.0% |
| !! | AATTAAGCTTTCTTTGAATA+AGG | + | chr6_1:75980343-75980362 | None:intergenic | 20.0% |
| !!! | TGTAATTGTTTTATGATGAA+TGG | - | chr6_1:75980821-75980840 | Msa0893140:intron | 20.0% |
| ! | GTTTGGATGAAATTGAAATT+GGG | + | chr6_1:75980251-75980270 | None:intergenic | 25.0% |
| ! | AGAAAGCTTAATTGAAAGTA+TGG | - | chr6_1:75980350-75980369 | Msa0893140:intron | 25.0% |
| !! | GAAAGCTTAATTGAAAGTAT+GGG | - | chr6_1:75980351-75980370 | Msa0893140:intron | 25.0% |
| !! | AAAGCTTAATTGAAAGTATG+GGG | - | chr6_1:75980352-75980371 | Msa0893140:intron | 25.0% |
| ! | TGTAAATCATTTCTGTTTGA+TGG | - | chr6_1:75980437-75980456 | Msa0893140:intron | 25.0% |
| ! | TCTGTTTAAGAAGATACATA+GGG | - | chr6_1:75980486-75980505 | Msa0893140:intron | 25.0% |
| ! | AAAAAGCTATTTGACAATAC+TGG | + | chr6_1:75980608-75980627 | None:intergenic | 25.0% |
| ! | TATGATAATTTCATCCCATA+AGG | - | chr6_1:75980735-75980754 | Msa0893140:intron | 25.0% |
| ! | TTACATAGAACATACCTTAT+GGG | + | chr6_1:75980752-75980771 | None:intergenic | 25.0% |
| ! | TTTACATAGAACATACCTTA+TGG | + | chr6_1:75980753-75980772 | None:intergenic | 25.0% |
| !! | TCTGAAAATCAATTTGTTTC+AGG | - | chr6_1:75980844-75980863 | Msa0893140:intron | 25.0% |
| !! | TGTAATTTTCAGTTAAGTTC+AGG | - | chr6_1:75980927-75980946 | Msa0893140:intron | 25.0% |
| ! | GTTTGGATGAAATTGAAATT+GGG | + | chr6_1:75980251-75980270 | None:intergenic | 25.0% |
| ! | AGAAAGCTTAATTGAAAGTA+TGG | - | chr6_1:75980350-75980369 | Msa0893140:intron | 25.0% |
| !! | GAAAGCTTAATTGAAAGTAT+GGG | - | chr6_1:75980351-75980370 | Msa0893140:intron | 25.0% |
| !! | AAAGCTTAATTGAAAGTATG+GGG | - | chr6_1:75980352-75980371 | Msa0893140:intron | 25.0% |
| ! | TGTAAATCATTTCTGTTTGA+TGG | - | chr6_1:75980437-75980456 | Msa0893140:intron | 25.0% |
| ! | TCTGTTTAAGAAGATACATA+GGG | - | chr6_1:75980486-75980505 | Msa0893140:intron | 25.0% |
| ! | AAAAAGCTATTTGACAATAC+TGG | + | chr6_1:75980608-75980627 | None:intergenic | 25.0% |
| ! | TATGATAATTTCATCCCATA+AGG | - | chr6_1:75980735-75980754 | Msa0893140:intron | 25.0% |
| ! | TTACATAGAACATACCTTAT+GGG | + | chr6_1:75980752-75980771 | None:intergenic | 25.0% |
| ! | TTTACATAGAACATACCTTA+TGG | + | chr6_1:75980753-75980772 | None:intergenic | 25.0% |
| !! | TCTGAAAATCAATTTGTTTC+AGG | - | chr6_1:75980844-75980863 | Msa0893140:intron | 25.0% |
| !! | TGTAATTTTCAGTTAAGTTC+AGG | - | chr6_1:75980927-75980946 | Msa0893140:intron | 25.0% |
| GGTTTGGATGAAATTGAAAT+TGG | + | chr6_1:75980252-75980271 | None:intergenic | 30.0% | |
| ! | AAGCTTAATTGAAAGTATGG+GGG | - | chr6_1:75980353-75980372 | Msa0893140:intron | 30.0% |
| GTCTGTTTAAGAAGATACAT+AGG | - | chr6_1:75980485-75980504 | Msa0893140:intron | 30.0% | |
| ATGGTTGAATTTGATCTGAA+TGG | - | chr6_1:75980511-75980530 | Msa0893140:intron | 30.0% | |
| AGATCAGATACAAAGGAAAA+TGG | + | chr6_1:75980573-75980592 | None:intergenic | 30.0% | |
| ! | ATTTTCCTTTGTATCTGATC+TGG | - | chr6_1:75980572-75980591 | Msa0893140:intron | 30.0% |
| ! | TTTTACCAGATCAGATACAA+AGG | + | chr6_1:75980580-75980599 | None:intergenic | 30.0% |
| GGTTTGGATGAAATTGAAAT+TGG | + | chr6_1:75980252-75980271 | None:intergenic | 30.0% | |
| ! | AAGCTTAATTGAAAGTATGG+GGG | - | chr6_1:75980353-75980372 | Msa0893140:intron | 30.0% |
| GTCTGTTTAAGAAGATACAT+AGG | - | chr6_1:75980485-75980504 | Msa0893140:intron | 30.0% | |
| ATGGTTGAATTTGATCTGAA+TGG | - | chr6_1:75980511-75980530 | Msa0893140:intron | 30.0% | |
| AGATCAGATACAAAGGAAAA+TGG | + | chr6_1:75980573-75980592 | None:intergenic | 30.0% | |
| ! | ATTTTCCTTTGTATCTGATC+TGG | - | chr6_1:75980572-75980591 | Msa0893140:intron | 30.0% |
| ! | TTTTACCAGATCAGATACAA+AGG | + | chr6_1:75980580-75980599 | None:intergenic | 30.0% |
| AGAAAAACAGAACGAGAGAA+TGG | - | chr6_1:75980127-75980146 | Msa0893140:CDS | 35.0% | |
| ! | GAAGGCAAGCTTCAATTTTA+AGG | - | chr6_1:75980205-75980224 | Msa0893140:intron | 35.0% |
| TGAAATTGGGCTGATAGAAA+GGG | + | chr6_1:75980238-75980257 | None:intergenic | 35.0% | |
| TTGAAATTGGGCTGATAGAA+AGG | + | chr6_1:75980239-75980258 | None:intergenic | 35.0% | |
| ATTGAAATTGGGGCTGAAAA+GGG | + | chr6_1:75980279-75980298 | None:intergenic | 35.0% | |
| AATTGAAATTGGGGCTGAAA+AGG | + | chr6_1:75980280-75980299 | None:intergenic | 35.0% | |
| ! | TTAATTGAAAGTATGGGGGT+TGG | - | chr6_1:75980357-75980376 | Msa0893140:intron | 35.0% |
| ! | TAATTGAAAGTATGGGGGTT+GGG | - | chr6_1:75980358-75980377 | Msa0893140:intron | 35.0% |
| GAGGAAGAAACAAGAAGAAT+TGG | - | chr6_1:75980391-75980410 | Msa0893140:intron | 35.0% | |
| GAAGAAACAAGAAGAATTGG+AGG | - | chr6_1:75980394-75980413 | Msa0893140:intron | 35.0% | |
| TAAGAAGATACATAGGGACA+TGG | - | chr6_1:75980492-75980511 | Msa0893140:intron | 35.0% | |
| GTGTCATCACTCAACATTAA+AGG | + | chr6_1:75980875-75980894 | None:intergenic | 35.0% | |
| TAATGTTGAGTGATGACACT+AGG | - | chr6_1:75980876-75980895 | Msa0893140:intron | 35.0% | |
| ! | TTGAATTTTTCACCTCTCTG+TGG | + | chr6_1:75980973-75980992 | None:intergenic | 35.0% |
| AGAAAAACAGAACGAGAGAA+TGG | - | chr6_1:75980127-75980146 | Msa0893140:CDS | 35.0% | |
| ! | GAAGGCAAGCTTCAATTTTA+AGG | - | chr6_1:75980205-75980224 | Msa0893140:intron | 35.0% |
| TGAAATTGGGCTGATAGAAA+GGG | + | chr6_1:75980238-75980257 | None:intergenic | 35.0% | |
| TTGAAATTGGGCTGATAGAA+AGG | + | chr6_1:75980239-75980258 | None:intergenic | 35.0% | |
| ATTGAAATTGGGGCTGAAAA+GGG | + | chr6_1:75980279-75980298 | None:intergenic | 35.0% | |
| AATTGAAATTGGGGCTGAAA+AGG | + | chr6_1:75980280-75980299 | None:intergenic | 35.0% | |
| ! | TTAATTGAAAGTATGGGGGT+TGG | - | chr6_1:75980357-75980376 | Msa0893140:intron | 35.0% |
| ! | TAATTGAAAGTATGGGGGTT+GGG | - | chr6_1:75980358-75980377 | Msa0893140:intron | 35.0% |
| GAGGAAGAAACAAGAAGAAT+TGG | - | chr6_1:75980391-75980410 | Msa0893140:intron | 35.0% | |
| GAAGAAACAAGAAGAATTGG+AGG | - | chr6_1:75980394-75980413 | Msa0893140:intron | 35.0% | |
| TAAGAAGATACATAGGGACA+TGG | - | chr6_1:75980492-75980511 | Msa0893140:intron | 35.0% | |
| GTGTCATCACTCAACATTAA+AGG | + | chr6_1:75980875-75980894 | None:intergenic | 35.0% | |
| TAATGTTGAGTGATGACACT+AGG | - | chr6_1:75980876-75980895 | Msa0893140:intron | 35.0% | |
| ! | TTGAATTTTTCACCTCTCTG+TGG | + | chr6_1:75980973-75980992 | None:intergenic | 35.0% |
| AAAACAGAACGAGAGAATGG+AGG | - | chr6_1:75980130-75980149 | Msa0893140:CDS | 40.0% | |
| AAACAGAACGAGAGAATGGA+GGG | - | chr6_1:75980131-75980150 | Msa0893140:CDS | 40.0% | |
| AATTTCCCTTCTTCCTCCAT+CGG | + | chr6_1:75980179-75980198 | None:intergenic | 40.0% | |
| AGAAGGGAAATTGGAGAAGA+AGG | - | chr6_1:75980187-75980206 | Msa0893140:CDS | 40.0% | |
| ! | TTGATCTGAATGGTGCTGAA+TGG | - | chr6_1:75980521-75980540 | Msa0893140:intron | 40.0% |
| AGCATCGTACTACGATATGA+AGG | - | chr6_1:75981029-75981048 | Msa0893140:intron | 40.0% | |
| !!! | ACTACGATATGAAGGCGTTT+TGG | - | chr6_1:75981037-75981056 | Msa0893140:CDS | 40.0% |
| TTCATCGTACTACGATGTCT+AGG | - | chr6_1:75981099-75981118 | Msa0893140:exon | 40.0% | |
| AAAACAGAACGAGAGAATGG+AGG | - | chr6_1:75980130-75980149 | Msa0893140:CDS | 40.0% | |
| AAACAGAACGAGAGAATGGA+GGG | - | chr6_1:75980131-75980150 | Msa0893140:CDS | 40.0% | |
| AATTTCCCTTCTTCCTCCAT+CGG | + | chr6_1:75980179-75980198 | None:intergenic | 40.0% | |
| AGAAGGGAAATTGGAGAAGA+AGG | - | chr6_1:75980187-75980206 | Msa0893140:CDS | 40.0% | |
| ! | TTGATCTGAATGGTGCTGAA+TGG | - | chr6_1:75980521-75980540 | Msa0893140:intron | 40.0% |
| AGCATCGTACTACGATATGA+AGG | - | chr6_1:75981029-75981048 | Msa0893140:intron | 40.0% | |
| !!! | ACTACGATATGAAGGCGTTT+TGG | - | chr6_1:75981037-75981056 | Msa0893140:CDS | 40.0% |
| TTCATCGTACTACGATGTCT+AGG | - | chr6_1:75981099-75981118 | Msa0893140:exon | 40.0% | |
| GAAGAAGGAAAAGAAGCCGA+TGG | - | chr6_1:75980160-75980179 | Msa0893140:CDS | 45.0% | |
| GATGGAGGAAGAAGGGAAAT+TGG | - | chr6_1:75980178-75980197 | Msa0893140:CDS | 45.0% | |
| ATTGGGGCTGAAAAGGGTTA+GGG | + | chr6_1:75980273-75980292 | None:intergenic | 45.0% | |
| AATTGGGGCTGAAAAGGGTT+AGG | + | chr6_1:75980274-75980293 | None:intergenic | 45.0% | |
| ATGAGTTATGAGCCACAGAG+AGG | - | chr6_1:75980958-75980977 | Msa0893140:intron | 45.0% | |
| TGCTACAGATCGTAGTACGA+TGG | + | chr6_1:75981013-75981032 | None:intergenic | 45.0% | |
| !!! | CGATATGAAGGCGTTTTGGA+AGG | - | chr6_1:75981041-75981060 | Msa0893140:CDS | 45.0% |
| !! | AGGAGCGTTGATTGATGATC+TGG | - | chr6_1:75981119-75981138 | Msa0893140:five_prime_UTR | 45.0% |
| GAAGAAGGAAAAGAAGCCGA+TGG | - | chr6_1:75980160-75980179 | Msa0893140:CDS | 45.0% | |
| GATGGAGGAAGAAGGGAAAT+TGG | - | chr6_1:75980178-75980197 | Msa0893140:CDS | 45.0% | |
| ATTGGGGCTGAAAAGGGTTA+GGG | + | chr6_1:75980273-75980292 | None:intergenic | 45.0% | |
| AATTGGGGCTGAAAAGGGTT+AGG | + | chr6_1:75980274-75980293 | None:intergenic | 45.0% | |
| ATGAGTTATGAGCCACAGAG+AGG | - | chr6_1:75980958-75980977 | Msa0893140:intron | 45.0% | |
| TGCTACAGATCGTAGTACGA+TGG | + | chr6_1:75981013-75981032 | None:intergenic | 45.0% | |
| !!! | CGATATGAAGGCGTTTTGGA+AGG | - | chr6_1:75981041-75981060 | Msa0893140:CDS | 45.0% |
| !! | AGGAGCGTTGATTGATGATC+TGG | - | chr6_1:75981119-75981138 | Msa0893140:five_prime_UTR | 45.0% |
| AATGGAGGGAGAGGAGAAGA+AGG | - | chr6_1:75980145-75980164 | Msa0893140:CDS | 50.0% | |
| GAAGGAAAAGAAGCCGATGG+AGG | - | chr6_1:75980163-75980182 | Msa0893140:CDS | 50.0% | |
| AAGAAGCCGATGGAGGAAGA+AGG | - | chr6_1:75980170-75980189 | Msa0893140:CDS | 50.0% | |
| AGAAGCCGATGGAGGAAGAA+GGG | - | chr6_1:75980171-75980190 | Msa0893140:CDS | 50.0% | |
| GGCTGAAAAGGGTTAGGGTT+TGG | + | chr6_1:75980268-75980287 | None:intergenic | 50.0% | |
| ATGGGGGTTGGGTTTGCAAT+TGG | - | chr6_1:75980369-75980388 | Msa0893140:intron | 50.0% | |
| AATGGAGGGAGAGGAGAAGA+AGG | - | chr6_1:75980145-75980164 | Msa0893140:CDS | 50.0% | |
| GAAGGAAAAGAAGCCGATGG+AGG | - | chr6_1:75980163-75980182 | Msa0893140:CDS | 50.0% | |
| AAGAAGCCGATGGAGGAAGA+AGG | - | chr6_1:75980170-75980189 | Msa0893140:CDS | 50.0% | |
| AGAAGCCGATGGAGGAAGAA+GGG | - | chr6_1:75980171-75980190 | Msa0893140:CDS | 50.0% | |
| GGCTGAAAAGGGTTAGGGTT+TGG | + | chr6_1:75980268-75980287 | None:intergenic | 50.0% | |
| ATGGGGGTTGGGTTTGCAAT+TGG | - | chr6_1:75980369-75980388 | Msa0893140:intron | 50.0% | |
| GAACGAGAGAATGGAGGGAG+AGG | - | chr6_1:75980136-75980155 | Msa0893140:CDS | 55.0% | |
| GGGGTTGGGTTTGCAATTGG+AGG | - | chr6_1:75980372-75980391 | Msa0893140:intron | 55.0% | |
| !!! | GAAGGCGTTTTGGAAGGCTG+AGG | - | chr6_1:75981047-75981066 | Msa0893140:CDS | 55.0% |
| GAACGAGAGAATGGAGGGAG+AGG | - | chr6_1:75980136-75980155 | Msa0893140:CDS | 55.0% | |
| GGGGTTGGGTTTGCAATTGG+AGG | - | chr6_1:75980372-75980391 | Msa0893140:intron | 55.0% | |
| !!! | GAAGGCGTTTTGGAAGGCTG+AGG | - | chr6_1:75981047-75981066 | Msa0893140:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 75980117 | 75981146 | 75980117 | ID=Msa0893140;Name=Msa0893140 |
| chr6_1 | mRNA | 75980117 | 75981146 | 75980117 | ID=Msa0893140-mRNA-1;Parent=Msa0893140;Name=Msa0893140-mRNA-1;_AED=0.59;_eAED=0.59;_QI=29|0|0.5|0.5|1|1|2|0|58 |
| chr6_1 | exon | 75980117 | 75980212 | 75980117 | ID=Msa0893140-mRNA-1:exon:12755;Parent=Msa0893140-mRNA-1 |
| chr6_1 | exon | 75981037 | 75981146 | 75981037 | ID=Msa0893140-mRNA-1:exon:12754;Parent=Msa0893140-mRNA-1 |
| chr6_1 | five_prime_UTR | 75981118 | 75981146 | 75981118 | ID=Msa0893140-mRNA-1:five_prime_utr;Parent=Msa0893140-mRNA-1 |
| chr6_1 | CDS | 75981037 | 75981117 | 75981037 | ID=Msa0893140-mRNA-1:cds;Parent=Msa0893140-mRNA-1 |
| chr6_1 | CDS | 75980117 | 75980212 | 75980117 | ID=Msa0893140-mRNA-1:cds;Parent=Msa0893140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
