AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0901620


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0901620 AT4G18400.1 66.667 81 24 2 25 102 27 107 1.35e-29 102

Find 30 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGAAAATACATAGATTT+TGG 0.159861 6_1:+98374123 None:intergenic
TTTACATCCTCTTGATTAAT+TGG 0.166261 6_1:+98374163 None:intergenic
TTTCTTCTCCAACAAATTAA+AGG 0.200250 6_1:-98374271 Msa0901620:three_prime_UTR
TCAATATTGTCAAGAACTTT+TGG 0.227238 6_1:-98376722 Msa0901620:CDS
GCTTACAATGGAATGAATAT+TGG 0.254169 6_1:-98374308 Msa0901620:three_prime_UTR
TTCATCTTCCTTTAATTTGT+TGG 0.289475 6_1:+98374263 None:intergenic
TTCATTCCATTGTAAGCAAT+TGG 0.326950 6_1:+98374314 None:intergenic
AATGGTGGGTCGGAAGGTGG+AGG 0.391509 6_1:+98376997 None:intergenic
TTTGGAACTTCAAACTAAAT+GGG 0.424588 6_1:-98376704 Msa0901620:CDS
AGAAGGAAAGTTACTTCGAC+TGG 0.431517 6_1:+98376963 None:intergenic
GTTTATTGACTTAAAGCCTC+TGG 0.442243 6_1:-98374889 Msa0901620:CDS
GGTAATGGTGGGTCGGAAGG+TGG 0.447434 6_1:+98376994 None:intergenic
TTACTTCGACTGGGATCAAA+TGG 0.451960 6_1:+98376973 None:intergenic
AGAGGCTTTAAGTCAATAAA+CGG 0.487243 6_1:+98374891 None:intergenic
AATGGTAATGGTGGGTCGGA+AGG 0.530646 6_1:+98376991 None:intergenic
CGACTGGGATCAAATGGTAA+TGG 0.539127 6_1:+98376979 None:intergenic
CGCAGAAGCTTCATCAATGG+TGG 0.550424 6_1:-98377085 Msa0901620:exon
ATAGTATATAGCAATTAAAG+AGG 0.552653 6_1:-98374228 Msa0901620:three_prime_UTR
ATGGTGGGTCGGAAGGTGGA+GGG 0.557898 6_1:+98376998 None:intergenic
CTGGGATCAAATGGTAATGG+TGG 0.558329 6_1:+98376982 None:intergenic
ACTTCAAACTAAATGGGAAG+AGG 0.567529 6_1:-98376698 Msa0901620:intron
TTCTAAGCTCTGAAGATATG+TGG 0.576788 6_1:-98374346 Msa0901620:three_prime_UTR
AGGCTTGCCAATTAATCAAG+AGG 0.580819 6_1:-98374170 Msa0901620:three_prime_UTR
ATCAAATGGTAATGGTGGGT+CGG 0.586109 6_1:+98376987 None:intergenic
ACTCGCAGAAGCTTCATCAA+TGG 0.594241 6_1:-98377088 None:intergenic
TATGCTCCAATTGCTTACAA+TGG 0.606083 6_1:-98374320 Msa0901620:three_prime_UTR
TATTGACTTAAAGCCTCTGG+AGG 0.611942 6_1:-98374886 Msa0901620:CDS
TGGGATCAAATGGTAATGGT+GGG 0.618040 6_1:+98376983 None:intergenic
GAAGGAAAGTTACTTCGACT+GGG 0.629018 6_1:+98376964 None:intergenic
TTGTTGGAGAAGAAAAGCTG+AGG 0.679207 6_1:+98374279 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAAAATTAATATCATATT+TGG + chr6_1:98376765-98376784 None:intergenic 10.0%
!! AGAAAATTGAAAATTGAAAA+GGG + chr6_1:98374261-98374280 None:intergenic 15.0%
!! AATTAAATTGTTTATCCAAT+AGG - chr6_1:98375759-98375778 Msa0901620:intron 15.0%
!! ATTTAATTACAAGTACTTTA+AGG - chr6_1:98376642-98376661 Msa0901620:intron 15.0%
!!! AATTTTTAGTTCAAGATTTT+AGG - chr6_1:98376681-98376700 Msa0901620:intron 15.0%
!!! TATTTTGTTAATTGTATTAG+AGG - chr6_1:98376997-98377016 Msa0901620:CDS 15.0%
!! GAGAAAATTGAAAATTGAAA+AGG + chr6_1:98374262-98374281 None:intergenic 20.0%
!!! AATTGAGTTTTACTGAATTT+AGG - chr6_1:98374286-98374305 Msa0901620:three_prime_UTR 20.0%
!!! ATTGAGTTTTACTGAATTTA+GGG - chr6_1:98374287-98374306 Msa0901620:three_prime_UTR 20.0%
!!! TATTTTTTGTTGAATTGAAG+TGG - chr6_1:98374378-98374397 Msa0901620:intron 20.0%
!!! ATTTAATTTATTTGCACTGT+TGG - chr6_1:98374552-98374571 Msa0901620:intron 20.0%
!!! GACTTGTTAGATTTATATAT+TGG - chr6_1:98374649-98374668 Msa0901620:intron 20.0%
!!! AAGTTGTTTTCATCTTATTT+TGG + chr6_1:98374820-98374839 None:intergenic 20.0%
!! TTTGGATAAATAGCTTAATT+AGG - chr6_1:98374924-98374943 Msa0901620:intron 20.0%
!! TTAAGCTATTTATCCTAATA+GGG + chr6_1:98375144-98375163 None:intergenic 20.0%
!! ATTAAGCTATTTATCCTAAT+AGG + chr6_1:98375145-98375164 None:intergenic 20.0%
!!! TATAAATTTGAAGCCATAAT+TGG - chr6_1:98376211-98376230 Msa0901620:intron 20.0%
!! ATTTGGATATAGAATAATGA+AGG + chr6_1:98376748-98376767 None:intergenic 20.0%
!! ATAGTATATAGCAATTAAAG+AGG - chr6_1:98376959-98376978 Msa0901620:intron 20.0%
!!! ACTTGAAAATACATAGATTT+TGG + chr6_1:98377067-98377086 None:intergenic 20.0%
!!! TCTTGTTCTTCTATTTTTGA+GGG + chr6_1:98374148-98374167 None:intergenic 25.0%
! ATTGAAAATTGAAAAGGGAA+TGG + chr6_1:98374256-98374275 None:intergenic 25.0%
! TACATTGCAATTATGAGTTT+AGG - chr6_1:98374321-98374340 Msa0901620:three_prime_UTR 25.0%
! ACATTGCAATTATGAGTTTA+GGG - chr6_1:98374322-98374341 Msa0901620:three_prime_UTR 25.0%
!! TCAATATTGTCAAGAACTTT+TGG - chr6_1:98374465-98374484 Msa0901620:intron 25.0%
!!! TTTTGGAACTTCAAACTAAA+TGG - chr6_1:98374482-98374501 Msa0901620:intron 25.0%
!! TTTGGAACTTCAAACTAAAT+GGG - chr6_1:98374483-98374502 Msa0901620:intron 25.0%
! TATAAAGGGATATAAAAGAG+TGG + chr6_1:98374527-98374546 None:intergenic 25.0%
!! GTGTTTTCAAATACATTACA+TGG + chr6_1:98374627-98374646 None:intergenic 25.0%
!! ATTTATATATTGGCTACGTT+TGG - chr6_1:98374659-98374678 Msa0901620:intron 25.0%
!! ACTGATTTCATAAACTATCT+CGG - chr6_1:98374786-98374805 Msa0901620:intron 25.0%
! ATTTAGAAACTAATAATGCC+CGG - chr6_1:98374893-98374912 Msa0901620:CDS 25.0%
! TAAACTAATAATGCCCTATT+AGG - chr6_1:98375128-98375147 Msa0901620:intron 25.0%
! TTATGTAAGTGCTTATGTAT+TGG - chr6_1:98375190-98375209 Msa0901620:intron 25.0%
! ATGAAAATAAATTGAAGAGC+AGG + chr6_1:98375388-98375407 None:intergenic 25.0%
!! TTTTCATCTTACATCGATAA+AGG + chr6_1:98375844-98375863 None:intergenic 25.0%
!!! ATTTTAGTGGTATTAGAAAG+AGG - chr6_1:98375986-98376005 Msa0901620:intron 25.0%
!! TTTTAGTGGTATTAGAAAGA+GGG - chr6_1:98375987-98376006 Msa0901620:intron 25.0%
! TGAAGGTAATGTGATTATAA+GGG + chr6_1:98376030-98376049 None:intergenic 25.0%
! ATGAAGGTAATGTGATTATA+AGG + chr6_1:98376031-98376050 None:intergenic 25.0%
! AGAAATATTACCCATTACAA+AGG - chr6_1:98376147-98376166 Msa0901620:intron 25.0%
! TGAAACACTATAACCAATTA+TGG + chr6_1:98376227-98376246 None:intergenic 25.0%
! TCAATAAGCTAAATAAGCTA+TGG + chr6_1:98376418-98376437 None:intergenic 25.0%
! AAGTACACAAAGTTAATATG+TGG - chr6_1:98376448-98376467 Msa0901620:intron 25.0%
! AAAGTTAATATGTGGATTCA+GGG - chr6_1:98376456-98376475 Msa0901620:intron 25.0%
! ATCTCACTGTAATAAACTAT+AGG + chr6_1:98376619-98376638 None:intergenic 25.0%
! TTTCTTCTCCAACAAATTAA+AGG - chr6_1:98376916-98376935 Msa0901620:intron 25.0%
!!! TTCATCTTCCTTTAATTTGT+TGG + chr6_1:98376927-98376946 None:intergenic 25.0%
! TTTACATCCTCTTGATTAAT+TGG + chr6_1:98377027-98377046 None:intergenic 25.0%
!!! CTCTTGTTCTTCTATTTTTG+AGG + chr6_1:98374149-98374168 None:intergenic 30.0%
AAAATTGAAAAGGGAATGGA+AGG + chr6_1:98374252-98374271 None:intergenic 30.0%
! TTTTGTTGAATTGAAGTGGT+TGG - chr6_1:98374382-98374401 Msa0901620:intron 30.0%
TTCAAATACATTACATGGTG+TGG + chr6_1:98374622-98374641 None:intergenic 30.0%
TATGCTATAAGTTGCAACTA+AGG + chr6_1:98374692-98374711 None:intergenic 30.0%
ATTTATCCAAAGTTCCAAAC+CGG + chr6_1:98374915-98374934 None:intergenic 30.0%
TCAAATAAGTCAATCCAAAC+AGG + chr6_1:98375296-98375315 None:intergenic 30.0%
TATTAACATAACCACTTGTG+AGG - chr6_1:98375323-98375342 Msa0901620:intron 30.0%
!! TTTATTTTCATATGCTCTCC+AGG - chr6_1:98375397-98375416 Msa0901620:intron 30.0%
!!! GTTGTTTTTATGAGTTATCC+TGG + chr6_1:98375418-98375437 None:intergenic 30.0%
TTGGAATAACTTACGAAAAC+AGG - chr6_1:98375564-98375583 Msa0901620:intron 30.0%
! GTTTCTTGTTAGCATTTTAG+TGG - chr6_1:98375973-98375992 Msa0901620:intron 30.0%
GACATTTCACAACATAATGA+AGG + chr6_1:98376047-98376066 None:intergenic 30.0%
TAAGATAAGCTGTGATCATA+TGG - chr6_1:98376078-98376097 Msa0901620:intron 30.0%
!! AGAGGCTTTAAGTCAATAAA+CGG + chr6_1:98376299-98376318 None:intergenic 30.0%
!!! AACTTTTTCATGCGTTTAGA+GGG + chr6_1:98376350-98376369 None:intergenic 30.0%
CAAAGTTAATATGTGGATTC+AGG - chr6_1:98376455-98376474 Msa0901620:intron 30.0%
ACAAAAGCTGAATGCAATAA+TGG + chr6_1:98376536-98376555 None:intergenic 30.0%
TTCATTCCATTGTAAGCAAT+TGG + chr6_1:98376876-98376895 None:intergenic 30.0%
GCTTACAATGGAATGAATAT+TGG - chr6_1:98376879-98376898 Msa0901620:intron 30.0%
!!! TTTTCAAGTTTGATCTTCGA+TGG - chr6_1:98377078-98377097 Msa0901620:exon 30.0%
ACTTCAAACTAAATGGGAAG+AGG - chr6_1:98374489-98374508 Msa0901620:intron 35.0%
TTTATCCAAAGTTCCAAACC+GGG + chr6_1:98374914-98374933 None:intergenic 35.0%
AAGTAAGGTGAAAACAGTGT+AGG - chr6_1:98375066-98375085 Msa0901620:intron 35.0%
AGTAAGGTGAAAACAGTGTA+GGG - chr6_1:98375067-98375086 Msa0901620:intron 35.0%
TAGCGTATACATAAGCAGTT+AGG - chr6_1:98375484-98375503 Msa0901620:intron 35.0%
TAACTTACGAAAACAGGCTA+TGG - chr6_1:98375570-98375589 Msa0901620:intron 35.0%
!! GCTGTTTTCATAAGCTATGT+TGG + chr6_1:98375636-98375655 None:intergenic 35.0%
CTTATCATCTAAGCTCCTAT+TGG + chr6_1:98375777-98375796 None:intergenic 35.0%
ACATCGATAAAGGTTCTGTT+TGG + chr6_1:98375834-98375853 None:intergenic 35.0%
TGAGTACTCGTCAAACTATT+TGG + chr6_1:98375906-98375925 None:intergenic 35.0%
TAAATAAGTCGATCCAACAC+AGG - chr6_1:98375941-98375960 Msa0901620:intron 35.0%
CATTATGTTGTGAAATGTCG+TGG - chr6_1:98376048-98376067 Msa0901620:intron 35.0%
CCAAACAAAGCCTTTGTAAT+GGG + chr6_1:98376160-98376179 None:intergenic 35.0%
!! CCATTACAAAGGCTTTGTTT+GGG - chr6_1:98376158-98376177 Msa0901620:intron 35.0%
!! GTTTATTGACTTAAAGCCTC+TGG - chr6_1:98376298-98376317 Msa0901620:intron 35.0%
!!! CAACTTTTTCATGCGTTTAG+AGG + chr6_1:98376351-98376370 None:intergenic 35.0%
TTGTCAAGAACTTGTAACCA+TGG - chr6_1:98376552-98376571 Msa0901620:intron 35.0%
TTCATCTGATCATTCTACCA+TGG + chr6_1:98376572-98376591 None:intergenic 35.0%
!! TTCTAAGCTCTGAAGATATG+TGG - chr6_1:98376841-98376860 Msa0901620:intron 35.0%
TATGCTCCAATTGCTTACAA+TGG - chr6_1:98376867-98376886 Msa0901620:intron 35.0%
!! ATCAAATGGTAATGGTGGGT+CGG + chr6_1:98374203-98374222 None:intergenic 40.0%
!! TGGGATCAAATGGTAATGGT+GGG + chr6_1:98374207-98374226 None:intergenic 40.0%
TTACTTCGACTGGGATCAAA+TGG + chr6_1:98374217-98374236 None:intergenic 40.0%
GAAGGAAAGTTACTTCGACT+GGG + chr6_1:98374226-98374245 None:intergenic 40.0%
AGAAGGAAAGTTACTTCGAC+TGG + chr6_1:98374227-98374246 None:intergenic 40.0%
AAAGGGAATGGAAGGAAAGA+AGG + chr6_1:98374244-98374263 None:intergenic 40.0%
GAAACTAATAATGCCCGGTT+TGG - chr6_1:98374898-98374917 Msa0901620:intron 40.0%
ATAGAGAGTTTGAGCCTGTT+TGG - chr6_1:98375279-98375298 Msa0901620:intron 40.0%
TAACCACTTGTGAGGTTGTT+TGG - chr6_1:98375331-98375350 Msa0901620:intron 40.0%
TTTGGACTTGGAAGAGCTTA+TGG - chr6_1:98375349-98375368 Msa0901620:intron 40.0%
! ATAAGCAGTTAGGCACTGTT+TGG - chr6_1:98375494-98375513 Msa0901620:intron 40.0%
!! TAAGCACTTCTGAGACTGTT+TGG - chr6_1:98375545-98375564 Msa0901620:intron 40.0%
AAACAACTTACAGCCTGTGT+TGG + chr6_1:98375957-98375976 None:intergenic 40.0%
!! CCCATTACAAAGGCTTTGTT+TGG - chr6_1:98376157-98376176 Msa0901620:intron 40.0%
CCCAAACAAAGCCTTTGTAA+TGG + chr6_1:98376161-98376180 None:intergenic 40.0%
!! TATTGACTTAAAGCCTCTGG+AGG - chr6_1:98376301-98376320 Msa0901620:intron 40.0%
!!! TCCTTTTTTGATTCCTCCAG+AGG + chr6_1:98376317-98376336 None:intergenic 40.0%
AAGTTGCGCTAGGTATGATA+TGG - chr6_1:98376365-98376384 Msa0901620:intron 40.0%
TTGTTGGAGAAGAAAAGCTG+AGG + chr6_1:98376911-98376930 None:intergenic 40.0%
AGGCTTGCCAATTAATCAAG+AGG - chr6_1:98377017-98377036 Msa0901620:CDS 40.0%
!! CTGGGATCAAATGGTAATGG+TGG + chr6_1:98374208-98374227 None:intergenic 45.0%
CGACTGGGATCAAATGGTAA+TGG + chr6_1:98374211-98374230 None:intergenic 45.0%
ATGGTGTGGCAACACATGAT+TGG + chr6_1:98374608-98374627 None:intergenic 45.0%
!! TAATGCCCGGTTTGGAACTT+TGG - chr6_1:98374906-98374925 Msa0901620:intron 45.0%
AAGCTATCCTAGAGAGCTTG+TGG - chr6_1:98375043-98375062 Msa0901620:intron 45.0%
CTTACTTCCACAAGCTCTCT+AGG + chr6_1:98375053-98375072 None:intergenic 45.0%
CTAGAGAGCTTGTGGAAGTA+AGG - chr6_1:98375051-98375070 Msa0901620:intron 45.0%
AGTCCAAACAACCTCACAAG+TGG + chr6_1:98375337-98375356 None:intergenic 45.0%
CTTGTGAGGTTGTTTGGACT+TGG - chr6_1:98375337-98375356 Msa0901620:intron 45.0%
!! CTCAGAAGTGCTTATGTCAG+TGG + chr6_1:98375539-98375558 None:intergenic 45.0%
! GCCTCTGGAGGAATCAAAAA+AGG - chr6_1:98376313-98376332 Msa0901620:intron 45.0%
ACGCATGAAAAAGTTGCGCT+AGG - chr6_1:98376355-98376374 Msa0901620:intron 45.0%
!! AAATTAAATTCATATATAAA+GGG + chr6_1:98374541-98374560 None:intergenic 5.0%
!! TAAATTAAATTCATATATAA+AGG + chr6_1:98374542-98374561 None:intergenic 5.0%
!! GATTAATTTATTTATTTATT+TGG - chr6_1:98376808-98376827 Msa0901620:intron 5.0%
!! AATGGTAATGGTGGGTCGGA+AGG + chr6_1:98374199-98374218 None:intergenic 50.0%
ATGCGTTTAGAGGGACGCGT+TGG + chr6_1:98376341-98376360 None:intergenic 55.0%
!! ATGGTGGGTCGGAAGGTGGA+GGG + chr6_1:98374192-98374211 None:intergenic 60.0%
!! AATGGTGGGTCGGAAGGTGG+AGG + chr6_1:98374193-98374212 None:intergenic 60.0%
!! GGTAATGGTGGGTCGGAAGG+TGG + chr6_1:98374196-98374215 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 98374105 98377104 98374105 ID=Msa0901620;Name=Msa0901620
chr6_1 mRNA 98374105 98377104 98374105 ID=Msa0901620-mRNA-1;Parent=Msa0901620;Name=Msa0901620-mRNA-1;_AED=0.00;_eAED=0.00;_QI=13|1|1|1|0.66|0.75|4|265|102
chr6_1 exon 98374105 98374369 98374105 ID=Msa0901620-mRNA-1:exon:17319;Parent=Msa0901620-mRNA-1
chr6_1 exon 98374833 98374913 98374833 ID=Msa0901620-mRNA-1:exon:17318;Parent=Msa0901620-mRNA-1
chr6_1 exon 98376699 98376811 98376699 ID=Msa0901620-mRNA-1:exon:17317;Parent=Msa0901620-mRNA-1
chr6_1 exon 98376977 98377104 98376977 ID=Msa0901620-mRNA-1:exon:17316;Parent=Msa0901620-mRNA-1
chr6_1 five_prime_UTR 98377092 98377104 98377092 ID=Msa0901620-mRNA-1:five_prime_utr;Parent=Msa0901620-mRNA-1
chr6_1 CDS 98376977 98377091 98376977 ID=Msa0901620-mRNA-1:cds;Parent=Msa0901620-mRNA-1
chr6_1 CDS 98376699 98376811 98376699 ID=Msa0901620-mRNA-1:cds;Parent=Msa0901620-mRNA-1
chr6_1 CDS 98374833 98374913 98374833 ID=Msa0901620-mRNA-1:cds;Parent=Msa0901620-mRNA-1
chr6_1 three_prime_UTR 98374105 98374369 98374105 ID=Msa0901620-mRNA-1:three_prime_utr;Parent=Msa0901620-mRNA-1
Gene Sequence

>Msa0901620

ATGGTGGAAGAAGAAGAACAAGAGCATCCCTCAAAAATAGAAGAACAAGAGCAAGAGCAAGAGCAAAATCTCCCTCCACCTTCCGACCCACCATTACCATTTGATCCCAGTCGAATGGTTGGTATCATCAAAAGAAAAGCTTTGATCAAAGACTTAGCTGCTGCATATCATGCTGAATGTCTTCAATATTGTCAAGAACTTTTGGAACTTCAAACTAAATGGGAAGAGCCGTTTATTGACTTAAAGCCTCTGGAGGAATCAAAAAAGGAGCCAACGCGTCCCTCTAAACGCATGAAAAAGTTGCGCTAG

Protein sequence

>Msa0901620

MVEEEEQEHPSKIEEQEQEQEQNLPPPSDPPLPFDPSRMVGIIKRKALIKDLAAAYHAECLQYCQELLELQTKWEEPFIDLKPLEESKKEPTRPSKRMKKLR*