Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0903760 | XP_003618418.1 | 92.708 | 192 | 9 | 1 | 1 | 187 | 1 | 192 | 3.12e-99 | 295 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0110970 | Msa0903760 | 0.803474 | 3.426054e-49 | -8.615850e-47 |
| Msa0727930 | Msa0903760 | 0.818443 | 1.955266e-52 | -8.615850e-47 |
| Msa0903760 | Msa0936410 | 0.851575 | 8.114402e-61 | -8.615850e-47 |
| Msa0903760 | Msa0973250 | 0.857715 | 1.350865e-62 | -8.615850e-47 |
| Msa0903760 | Msa1271260 | 0.804005 | 2.659066e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0903760 | MtrunA17_Chr6g0452191 | 92.708 | 192 | 9 | 1 | 1 | 187 | 1 | 192 | 2.87e-103 | 295 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0903760 | AT3G28420.1 | 32.597 | 181 | 92 | 3 | 19 | 169 | 11 | 191 | 4.71e-19 | 81.3 |
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCTACTATTCCAATAATT+TGG | 0.174987 | 6_2:+1168353 | None:intergenic |
| TGTCTATGAAGCCAAATTAT+TGG | 0.179250 | 6_2:-1168364 | Msa0903760:CDS |
| TTCATTGAACTCTTCCTCTT+TGG | 0.246225 | 6_2:+1168520 | None:intergenic |
| CTTAGGTACATTTCTTCTAA+TGG | 0.291653 | 6_2:-1168471 | Msa0903760:CDS |
| CAACAAGAAACTTCAATCTT+TGG | 0.300187 | 6_2:+1168017 | None:intergenic |
| AAGCTTTCATAGCTATGTTT+AGG | 0.353873 | 6_2:-1168106 | Msa0903760:CDS |
| ACATGAAAAGAATGGCTAAT+AGG | 0.384484 | 6_2:+1168384 | None:intergenic |
| TGATGAGGAAGAGGAGGAAG+AGG | 0.407935 | 6_2:-1168177 | Msa0903760:CDS |
| GAATGAGATTGATGAGGAAG+AGG | 0.437629 | 6_2:-1168186 | Msa0903760:CDS |
| ATAGGATGACATTGAGGATC+AGG | 0.447933 | 6_2:+1168402 | None:intergenic |
| ACATGTTGAAGTGTTGTCTA+AGG | 0.463908 | 6_2:-1168150 | Msa0903760:CDS |
| TGAGATTGATGAGGAAGAGG+AGG | 0.475585 | 6_2:-1168183 | Msa0903760:CDS |
| CTTCATAGACATGAAAAGAA+TGG | 0.497405 | 6_2:+1168376 | None:intergenic |
| ATTGAAGTTTCTTGTTGTTG+AGG | 0.509043 | 6_2:-1168011 | None:intergenic |
| AATGTTACAAACAACAATGT+TGG | 0.513485 | 6_2:-1168235 | Msa0903760:CDS |
| CAAACTATAATGTTCACCTT+AGG | 0.521506 | 6_2:-1168488 | Msa0903760:CDS |
| AGAGTTTCTTAGTTGAGAAG+TGG | 0.537952 | 6_2:-1168439 | Msa0903760:CDS |
| CATCAATCTCATTCTCAACA+TGG | 0.571952 | 6_2:+1168195 | None:intergenic |
| CATTAGAAGAAATGTACCTA+AGG | 0.572803 | 6_2:+1168472 | None:intergenic |
| TTGATTTCAGATGTTAAACA+AGG | 0.621029 | 6_2:-1168077 | Msa0903760:CDS |
| TGGCTAATAGGATGACATTG+AGG | 0.643978 | 6_2:+1168396 | None:intergenic |
| ATATGAAAGAGAAACCAAAG+AGG | 0.672376 | 6_2:-1168534 | Msa0903760:CDS |
| TGTTGAGAATGAGATTGATG+AGG | 0.707131 | 6_2:-1168192 | Msa0903760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCTACTATTCCAATAATT+TGG | + | chr6_2:1168217-1168236 | None:intergenic | 20.0% |
| ! | AATGTTACAAACAACAATGT+TGG | - | chr6_2:1168332-1168351 | Msa0903760:CDS | 25.0% |
| ! | TTGATTTCAGATGTTAAACA+AGG | - | chr6_2:1168490-1168509 | Msa0903760:CDS | 25.0% |
| !! | TTAAACAAGGTGAAGATTTT+AGG | - | chr6_2:1168503-1168522 | Msa0903760:CDS | 25.0% |
| !!! | GTGTCAAATTTGATGTTTTT+TGG | + | chr6_2:1168531-1168550 | None:intergenic | 25.0% |
| ATATGAAAGAGAAACCAAAG+AGG | - | chr6_2:1168033-1168052 | Msa0903760:CDS | 30.0% | |
| CAAACTATAATGTTCACCTT+AGG | - | chr6_2:1168079-1168098 | Msa0903760:CDS | 30.0% | |
| CATTAGAAGAAATGTACCTA+AGG | + | chr6_2:1168098-1168117 | None:intergenic | 30.0% | |
| CTTAGGTACATTTCTTCTAA+TGG | - | chr6_2:1168096-1168115 | Msa0903760:CDS | 30.0% | |
| ACATGAAAAGAATGGCTAAT+AGG | + | chr6_2:1168186-1168205 | None:intergenic | 30.0% | |
| CTTCATAGACATGAAAAGAA+TGG | + | chr6_2:1168194-1168213 | None:intergenic | 30.0% | |
| TGTCTATGAAGCCAAATTAT+TGG | - | chr6_2:1168203-1168222 | Msa0903760:CDS | 30.0% | |
| AAGCTTTCATAGCTATGTTT+AGG | - | chr6_2:1168461-1168480 | Msa0903760:CDS | 30.0% | |
| CAACAAGAAACTTCAATCTT+TGG | + | chr6_2:1168553-1168572 | None:intergenic | 30.0% | |
| TTCATTGAACTCTTCCTCTT+TGG | + | chr6_2:1168050-1168069 | None:intergenic | 35.0% | |
| AGAGTTTCTTAGTTGAGAAG+TGG | - | chr6_2:1168128-1168147 | Msa0903760:CDS | 35.0% | |
| CATCAATCTCATTCTCAACA+TGG | + | chr6_2:1168375-1168394 | None:intergenic | 35.0% | |
| TGTTGAGAATGAGATTGATG+AGG | - | chr6_2:1168375-1168394 | Msa0903760:CDS | 35.0% | |
| !! | ACATGTTGAAGTGTTGTCTA+AGG | - | chr6_2:1168417-1168436 | Msa0903760:CDS | 35.0% |
| ATAGGATGACATTGAGGATC+AGG | + | chr6_2:1168168-1168187 | None:intergenic | 40.0% | |
| TGGCTAATAGGATGACATTG+AGG | + | chr6_2:1168174-1168193 | None:intergenic | 40.0% | |
| GAATGAGATTGATGAGGAAG+AGG | - | chr6_2:1168381-1168400 | Msa0903760:CDS | 40.0% | |
| TGAGATTGATGAGGAAGAGG+AGG | - | chr6_2:1168384-1168403 | Msa0903760:CDS | 45.0% | |
| TGATGAGGAAGAGGAGGAAG+AGG | - | chr6_2:1168390-1168409 | Msa0903760:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 1168013 | 1168576 | 1168013 | ID=Msa0903760;Name=Msa0903760 |
| chr6_2 | mRNA | 1168013 | 1168576 | 1168013 | ID=Msa0903760-mRNA-1;Parent=Msa0903760;Name=Msa0903760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|187 |
| chr6_2 | exon | 1168013 | 1168576 | 1168013 | ID=Msa0903760-mRNA-1:exon:459;Parent=Msa0903760-mRNA-1 |
| chr6_2 | CDS | 1168013 | 1168576 | 1168013 | ID=Msa0903760-mRNA-1:cds;Parent=Msa0903760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
