AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0973250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0973250 MtrunA17_Chr6g0452191 89.583 192 12 2 1 184 1 192 3.20e-100 287
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0973250 AT3G28420.1 33.149 181 88 4 19 166 11 191 3.02e-18 79.3

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTACTATTCCAATAATT+TGG 0.174987 6_4:+5719759 None:intergenic
TGTCTATGAAGCCAAATTAT+TGG 0.179250 6_4:-5719770 Msa0973250:CDS
TTCATTGAACTCTTCCTCTT+TGG 0.246225 6_4:+5719926 None:intergenic
CTTAGGTACATTTCTTCTAA+TGG 0.291653 6_4:-5719877 Msa0973250:CDS
CAACAAGAAACTTCAATCTT+TGG 0.300187 6_4:+5719432 None:intergenic
AAGCTTTCATAGCTATGTTT+AGG 0.369886 6_4:-5719521 Msa0973250:CDS
ACATGAAAAGAATGGCTAAT+AGG 0.384484 6_4:+5719790 None:intergenic
GAATGAGATTGAGGAGGAAG+AGG 0.437629 6_4:-5719592 Msa0973250:CDS
ATAGGATGACATTGAGGATC+AGG 0.447933 6_4:+5719808 None:intergenic
ACATGTTGAAGTGTTGTCTA+AGG 0.463908 6_4:-5719565 Msa0973250:CDS
CTTCATAGACATGAAAAGAA+TGG 0.497405 6_4:+5719782 None:intergenic
GGAAGAATTGAATGAAAGAG+TGG 0.499912 6_4:-5719544 Msa0973250:CDS
ATTGAAGTTTCTTGTTGTTG+AGG 0.509043 6_4:-5719426 None:intergenic
AATATTACAAACAACAATGT+TGG 0.513485 6_4:-5719641 Msa0973250:CDS
CAAACTATAATGTTCACCTT+AGG 0.521506 6_4:-5719894 Msa0973250:CDS
AGAGTTTCTTAGTTGAGAAG+TGG 0.537952 6_4:-5719845 Msa0973250:CDS
CCTCAATCTCATTCTCAACA+TGG 0.570203 6_4:+5719601 None:intergenic
CATTAGAAGAAATGTACCTA+AGG 0.572803 6_4:+5719878 None:intergenic
CCATGTTGAGAATGAGATTG+AGG 0.593248 6_4:-5719601 Msa0973250:CDS
ATGTGAAAGAGAAACCAAAG+AGG 0.618564 6_4:-5719940 Msa0973250:CDS
TTGATTTCAGATGTTAAACA+AGG 0.621029 6_4:-5719492 Msa0973250:CDS
TGGCTAATAGGATGACATTG+AGG 0.643978 6_4:+5719802 None:intergenic
TGTTGAGAATGAGATTGAGG+AGG 0.668905 6_4:-5719598 Msa0973250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCTACTATTCCAATAATT+TGG + chr6_4:5719632-5719651 None:intergenic 20.0%
!! AATATTACAAACAACAATGT+TGG - chr6_4:5719747-5719766 Msa0973250:CDS 20.0%
! TTGATTTCAGATGTTAAACA+AGG - chr6_4:5719896-5719915 Msa0973250:CDS 25.0%
!! TTAAACAAGGTGAAGATTTT+AGG - chr6_4:5719909-5719928 Msa0973250:CDS 25.0%
!!! GTGTCAAATTTGATGTTTTT+TGG + chr6_4:5719937-5719956 None:intergenic 25.0%
CAAACTATAATGTTCACCTT+AGG - chr6_4:5719494-5719513 Msa0973250:CDS 30.0%
CATTAGAAGAAATGTACCTA+AGG + chr6_4:5719513-5719532 None:intergenic 30.0%
CTTAGGTACATTTCTTCTAA+TGG - chr6_4:5719511-5719530 Msa0973250:CDS 30.0%
ACATGAAAAGAATGGCTAAT+AGG + chr6_4:5719601-5719620 None:intergenic 30.0%
CTTCATAGACATGAAAAGAA+TGG + chr6_4:5719609-5719628 None:intergenic 30.0%
TGTCTATGAAGCCAAATTAT+TGG - chr6_4:5719618-5719637 Msa0973250:CDS 30.0%
AAGCTTTCATAGCTATGTTT+AGG - chr6_4:5719867-5719886 Msa0973250:CDS 30.0%
CAACAAGAAACTTCAATCTT+TGG + chr6_4:5719959-5719978 None:intergenic 30.0%
ATGTGAAAGAGAAACCAAAG+AGG - chr6_4:5719448-5719467 Msa0973250:CDS 35.0%
TTCATTGAACTCTTCCTCTT+TGG + chr6_4:5719465-5719484 None:intergenic 35.0%
AGAGTTTCTTAGTTGAGAAG+TGG - chr6_4:5719543-5719562 Msa0973250:CDS 35.0%
!! ACATGTTGAAGTGTTGTCTA+AGG - chr6_4:5719823-5719842 Msa0973250:CDS 35.0%
GGAAGAATTGAATGAAAGAG+TGG - chr6_4:5719844-5719863 Msa0973250:CDS 35.0%
ATAGGATGACATTGAGGATC+AGG + chr6_4:5719583-5719602 None:intergenic 40.0%
TGGCTAATAGGATGACATTG+AGG + chr6_4:5719589-5719608 None:intergenic 40.0%
CCTCAATCTCATTCTCAACA+TGG + chr6_4:5719790-5719809 None:intergenic 40.0%
CCATGTTGAGAATGAGATTG+AGG - chr6_4:5719787-5719806 Msa0973250:CDS 40.0%
TGTTGAGAATGAGATTGAGG+AGG - chr6_4:5719790-5719809 Msa0973250:CDS 40.0%
GAATGAGATTGAGGAGGAAG+AGG - chr6_4:5719796-5719815 Msa0973250:CDS 45.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 5719428 5719982 5719428 ID=Msa0973250;Name=Msa0973250
chr6_4 mRNA 5719428 5719982 5719428 ID=Msa0973250-mRNA-1;Parent=Msa0973250;Name=Msa0973250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|184
chr6_4 exon 5719428 5719982 5719428 ID=Msa0973250-mRNA-1:exon:2420;Parent=Msa0973250-mRNA-1
chr6_4 CDS 5719428 5719982 5719428 ID=Msa0973250-mRNA-1:cds;Parent=Msa0973250-mRNA-1
Gene Sequence

>Msa0973250

ATGGAGATGAAGAAGAGAGAATGTGAAAGAGAAACCAAAGAGGAAGAGTTCAATGAAGATAGTATACAAACTATAATGTTCACCTTAGGTACATTTCTTCTAATGGTTTGTCTAAAGAGTTTCTTAGTTGAGAAGTGGCGTGCTTATATGTTCCTGATCCTCAATGTCATCCTATTAGCCATTCTTTTCATGTCTATGAAGCCAAATTATTGGAATAGTAGAAACTTAGAAAGTGAAAGCAATGTTGAAGAAGTGAAAATTGATGACAAATTGAAGAAAAGTGCATGTGAGTCTTCTCAAGAAATAGAAGAAAAAAAAGAATATTACAAACAACAATGTTGGACTAGTACTAATCATGTCCATGTTGAGAATGAGATTGAGGAGGAAGAGGATGAACATGTTGAAGTGTTGTCTAAGGAAGAATTGAATGAAAGAGTGGAAGCTTTCATAGCTATGTTTAGGAAGCATTTGATTTCAGATGTTAAACAAGGTGAAGATTTTAGGCACCAAAAAACATCAAATTTGACACCAAAGATTGAAGTTTCTTGTTGTTGA

Protein sequence

>Msa0973250

MEMKKRECERETKEEEFNEDSIQTIMFTLGTFLLMVCLKSFLVEKWRAYMFLILNVILLAILFMSMKPNYWNSRNLESESNVEEVKIDDKLKKSACESSQEIEEKKEYYKQQCWTSTNHVHVENEIEEEEDEHVEVLSKEELNERVEAFIAMFRKHLISDVKQGEDFRHQKTSNLTPKIEVSCC*