AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0936410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0936410 MtrunA17_Chr6g0452191 90.104 192 11 2 1 184 1 192 1.23e-101 290
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0936410 AT3G28420.1 33.149 181 88 4 19 166 11 191 4.00e-18 79.0

Find 23 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTACTATTCCAATAATT+TGG 0.174987 6_3:-7118124 None:intergenic
TGTCTATGAAGCCAAATTAT+TGG 0.179250 6_3:+7118113 Msa0936410:CDS
TTCATTGAACTCTTCCTCTT+TGG 0.246225 6_3:-7117957 None:intergenic
CTTAGGTACATTTCTTCTAA+TGG 0.291653 6_3:+7118006 Msa0936410:CDS
CAACAAGAAACTTCAATCTT+TGG 0.300187 6_3:-7118451 None:intergenic
AAGCTTTCATAGCTATGTTT+AGG 0.369886 6_3:+7118362 Msa0936410:CDS
ACATGAAAAGAATGGCTAAT+AGG 0.384484 6_3:-7118093 None:intergenic
GAATGAGATTGAGGAGGAAG+AGG 0.437629 6_3:+7118291 Msa0936410:CDS
ATAGGATGACATTGAGGATC+AGG 0.447933 6_3:-7118075 None:intergenic
ACATGTTGAAGTGTTGTCTA+AGG 0.463908 6_3:+7118318 Msa0936410:CDS
CTTCATAGACATGAAAAGAA+TGG 0.497405 6_3:-7118101 None:intergenic
GGAAGAATTGAATGAAAGAG+TGG 0.499912 6_3:+7118339 Msa0936410:CDS
ATTGAAGTTTCTTGTTGTTG+AGG 0.509043 6_3:+7118457 Msa0936410:CDS
AATGTTACAAACAACAATGT+TGG 0.513485 6_3:+7118242 Msa0936410:CDS
CAAACTATAATGTTCACCTT+AGG 0.521506 6_3:+7117989 Msa0936410:CDS
AGAGTTTCTTAGTTGAGAAG+TGG 0.537952 6_3:+7118038 Msa0936410:CDS
CCTCAATCTCATTCTCAACA+TGG 0.570203 6_3:-7118282 None:intergenic
CATTAGAAGAAATGTACCTA+AGG 0.572803 6_3:-7118005 None:intergenic
CCATGTTGAGAATGAGATTG+AGG 0.593248 6_3:+7118282 Msa0936410:CDS
ATGTGAAAGAGAAACCAAAG+AGG 0.618564 6_3:+7117943 Msa0936410:CDS
TTGATTTCAGATGTTAAACA+AGG 0.621029 6_3:+7118391 Msa0936410:CDS
TGGCTAATAGGATGACATTG+AGG 0.643978 6_3:-7118081 None:intergenic
TGTTGAGAATGAGATTGAGG+AGG 0.668905 6_3:+7118285 Msa0936410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCTACTATTCCAATAATT+TGG - chr6_3:7118127-7118146 None:intergenic 20.0%
! AATGTTACAAACAACAATGT+TGG + chr6_3:7118242-7118261 Msa0936410:CDS 25.0%
! TTGATTTCAGATGTTAAACA+AGG + chr6_3:7118391-7118410 Msa0936410:CDS 25.0%
!! TTAAACAAGGTGAAGATTTT+AGG + chr6_3:7118404-7118423 Msa0936410:CDS 25.0%
!!! GTGTCAAATTTGATGTTTTT+TGG - chr6_3:7118432-7118451 None:intergenic 25.0%
CAAACTATAATGTTCACCTT+AGG + chr6_3:7117989-7118008 Msa0936410:CDS 30.0%
CATTAGAAGAAATGTACCTA+AGG - chr6_3:7118008-7118027 None:intergenic 30.0%
CTTAGGTACATTTCTTCTAA+TGG + chr6_3:7118006-7118025 Msa0936410:CDS 30.0%
ACATGAAAAGAATGGCTAAT+AGG - chr6_3:7118096-7118115 None:intergenic 30.0%
CTTCATAGACATGAAAAGAA+TGG - chr6_3:7118104-7118123 None:intergenic 30.0%
TGTCTATGAAGCCAAATTAT+TGG + chr6_3:7118113-7118132 Msa0936410:CDS 30.0%
AAGCTTTCATAGCTATGTTT+AGG + chr6_3:7118362-7118381 Msa0936410:CDS 30.0%
CAACAAGAAACTTCAATCTT+TGG - chr6_3:7118454-7118473 None:intergenic 30.0%
ATGTGAAAGAGAAACCAAAG+AGG + chr6_3:7117943-7117962 Msa0936410:CDS 35.0%
TTCATTGAACTCTTCCTCTT+TGG - chr6_3:7117960-7117979 None:intergenic 35.0%
AGAGTTTCTTAGTTGAGAAG+TGG + chr6_3:7118038-7118057 Msa0936410:CDS 35.0%
!! ACATGTTGAAGTGTTGTCTA+AGG + chr6_3:7118318-7118337 Msa0936410:CDS 35.0%
GGAAGAATTGAATGAAAGAG+TGG + chr6_3:7118339-7118358 Msa0936410:CDS 35.0%
ATAGGATGACATTGAGGATC+AGG - chr6_3:7118078-7118097 None:intergenic 40.0%
TGGCTAATAGGATGACATTG+AGG - chr6_3:7118084-7118103 None:intergenic 40.0%
CCTCAATCTCATTCTCAACA+TGG - chr6_3:7118285-7118304 None:intergenic 40.0%
CCATGTTGAGAATGAGATTG+AGG + chr6_3:7118282-7118301 Msa0936410:CDS 40.0%
TGTTGAGAATGAGATTGAGG+AGG + chr6_3:7118285-7118304 Msa0936410:CDS 40.0%
GAATGAGATTGAGGAGGAAG+AGG + chr6_3:7118291-7118310 Msa0936410:CDS 45.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 7117923 7118477 7117923 ID=Msa0936410;Name=Msa0936410
chr6_3 mRNA 7117923 7118477 7117923 ID=Msa0936410-mRNA-1;Parent=Msa0936410;Name=Msa0936410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|184
chr6_3 exon 7117923 7118477 7117923 ID=Msa0936410-mRNA-1:exon:2309;Parent=Msa0936410-mRNA-1
chr6_3 CDS 7117923 7118477 7117923 ID=Msa0936410-mRNA-1:cds;Parent=Msa0936410-mRNA-1
Gene Sequence

>Msa0936410

ATGGAGATGAAGAAGAGAGAATGTGAAAGAGAAACCAAAGAGGAAGAGTTCAATGAAGATAGTATACAAACTATAATGTTCACCTTAGGTACATTTCTTCTAATGGTTTGTCTAAAGAGTTTCTTAGTTGAGAAGTGGCGTGCTTATATGTTCCTGATCCTCAATGTCATCCTATTAGCCATTCTTTTCATGTCTATGAAGCCAAATTATTGGAATAGTAGAAACTTAGAAAGTGAAAGCAATGTTGAAGAAGTGAAAATTGATGACAAATTGAAGAAAAGTGCATGTGAGTCTTCTCAAGAAATAGAAGAAAAAAAAGAATGTTACAAACAACAATGTTGGACTAGTACTAATCATGTCCATGTTGAGAATGAGATTGAGGAGGAAGAGGATGAACATGTTGAAGTGTTGTCTAAGGAAGAATTGAATGAAAGAGTGGAAGCTTTCATAGCTATGTTTAGGAAGCATTTGATTTCAGATGTTAAACAAGGTGAAGATTTTAGGCACCAAAAAACATCAAATTTGACACCAAAGATTGAAGTTTCTTGTTGTTGA

Protein sequence

>Msa0936410

MEMKKRECERETKEEEFNEDSIQTIMFTLGTFLLMVCLKSFLVEKWRAYMFLILNVILLAILFMSMKPNYWNSRNLESESNVEEVKIDDKLKKSACESSQEIEEKKECYKQQCWTSTNHVHVENEIEEEEDEHVEVLSKEELNERVEAFIAMFRKHLISDVKQGEDFRHQKTSNLTPKIEVSCC*