AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0920680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0920680 MtrunA17_Chr6g0465931 61.342 313 77 3 1 299 56 338 9.02e-130 376
Msa0920680 MtrunA17_Chr6g0466111 61.342 313 77 3 1 299 56 338 1.21e-129 376
Msa0920680 MtrunA17_Chr6g0465981 61.342 313 77 3 1 299 91 373 4.15e-129 376
Msa0920680 MtrunA17_Chr6g0465871 61.342 313 77 3 1 299 91 373 4.15e-129 376
Msa0920680 MtrunA17_Chr6g0466081 61.342 313 77 3 1 299 91 373 4.15e-129 376
Msa0920680 MtrunA17_Chr7g0236951 60.065 308 79 3 1 294 36 313 9.82e-129 373
Msa0920680 MtrunA17_Chr6g0466051 61.392 316 78 3 1 302 91 376 4.85e-128 379
Msa0920680 MtrunA17_Chr6g0466051 62.245 98 37 0 299 396 456 553 5.51e-38 144
Msa0920680 MtrunA17_Chr7g0237031 59.740 308 80 3 1 294 36 313 5.94e-128 371
Msa0920680 MtrunA17_Chr6g0465851 61.076 316 79 3 1 302 56 341 9.51e-128 377
Msa0920680 MtrunA17_Chr6g0465851 62.245 98 37 0 299 396 421 518 3.57e-38 144
Msa0920680 MtrunA17_Chr5g0425371 59.683 315 83 3 1 301 91 375 2.60e-127 377
Msa0920680 MtrunA17_Chr5g0425371 65.306 98 34 0 299 396 456 553 9.43e-38 143
Msa0920680 MtrunA17_Chr6g0465911 61.076 316 79 3 1 302 91 376 3.12e-127 377
Msa0920680 MtrunA17_Chr6g0465911 62.245 98 37 0 299 396 456 553 5.46e-38 144
Msa0920680 MtrunA17_Chr7g0236961 59.177 316 85 3 1 302 36 321 5.15e-127 375
Msa0920680 MtrunA17_Chr7g0236961 67.347 98 32 0 299 396 401 498 4.48e-41 151
Msa0920680 MtrunA17_Chr7g0236991 59.177 316 85 3 1 302 91 376 2.88e-126 375
Msa0920680 MtrunA17_Chr7g0236991 67.347 98 32 0 299 396 456 553 8.83e-41 151
Msa0920680 MtrunA17_Chr7g0237011 58.861 316 86 3 1 302 36 321 2.93e-126 373
Msa0920680 MtrunA17_Chr7g0237011 67.347 98 32 0 299 396 401 498 4.39e-41 151
Msa0920680 MtrunA17_Chr7g0237001 58.861 316 86 3 1 302 91 376 1.31e-125 373
Msa0920680 MtrunA17_Chr7g0237001 67.347 98 32 0 299 396 456 553 8.07e-41 151
Msa0920680 MtrunA17_Chr7g0237041 58.861 316 86 3 1 302 91 376 1.31e-125 373
Msa0920680 MtrunA17_Chr7g0237041 67.347 98 32 0 299 396 456 553 8.07e-41 151
Msa0920680 MtrunA17_Chr7g0237081 58.861 316 86 3 1 302 91 376 1.31e-125 373
Msa0920680 MtrunA17_Chr7g0237081 67.347 98 32 0 299 396 456 553 8.07e-41 151
Msa0920680 MtrunA17_Chr7g0237051 58.861 316 86 3 1 302 91 376 1.31e-125 373
Msa0920680 MtrunA17_Chr7g0237051 67.347 98 32 0 299 396 456 553 8.07e-41 151
Msa0920680 MtrunA17_Chr6g0465441 58.861 316 86 3 1 302 91 376 7.00e-123 366
Msa0920680 MtrunA17_Chr6g0465441 65.306 98 34 0 299 396 456 553 9.99e-40 149
Msa0920680 MtrunA17_Chr6g0451711 57.778 315 89 3 1 301 95 379 3.76e-120 359
Msa0920680 MtrunA17_Chr6g0451711 64.286 98 35 0 299 396 460 557 4.91e-38 144
Msa0920680 MtrunA17_Chr3g0082691 56.839 329 90 5 4 317 93 384 1.41e-118 355
Msa0920680 MtrunA17_Chr3g0082691 65.306 98 34 0 299 396 456 553 4.53e-40 149
Msa0920680 MtrunA17_Chr3g0105071 55.856 333 93 6 1 317 94 388 1.52e-116 350
Msa0920680 MtrunA17_Chr3g0105071 66.327 98 33 0 299 396 460 557 1.87e-37 142
Msa0920680 MtrunA17_Chr5g0431651 54.354 333 98 6 1 317 89 383 1.06e-112 340
Msa0920680 MtrunA17_Chr5g0431651 63.265 98 36 0 299 396 455 552 1.25e-37 142
Msa0920680 MtrunA17_Chr5g0431571 56.024 332 94 5 1 317 86 380 1.01e-111 337
Msa0920680 MtrunA17_Chr5g0431571 63.265 98 36 0 299 396 452 549 1.97e-38 145
Msa0920680 MtrunA17_Chr4g0024691 55.096 314 97 3 3 302 101 384 5.77e-109 331
Msa0920680 MtrunA17_Chr4g0024691 69.388 98 30 0 299 396 464 561 1.68e-40 150
Msa0920680 MtrunA17_Chr4g0042111 52.978 319 102 5 1 304 100 385 1.47e-108 330
Msa0920680 MtrunA17_Chr4g0042111 53.061 98 46 0 299 396 465 562 4.19e-27 113
Msa0920680 MtrunA17_Chr5g0445511 56.641 256 65 4 59 299 2 226 1.67e-91 275
Msa0920680 MtrunA17_Chr7g0236971 55.906 254 68 3 63 302 1 224 6.36e-91 279
Msa0920680 MtrunA17_Chr7g0236971 67.347 98 32 0 299 396 304 401 8.22e-42 151
Msa0920680 MtrunA17_Chr2g0319051 40.000 315 143 5 1 299 50 334 5.38e-78 249
Msa0920680 MtrunA17_Chr2g0319051 42.424 99 55 2 299 396 417 514 9.67e-19 87.8
Msa0920680 MtrunA17_Chr8g0336181 42.424 297 125 4 1 281 91 357 1.86e-75 244
Msa0920680 MtrunA17_Chr8g0336181 40.404 99 57 2 299 396 459 556 9.13e-17 82.0
Msa0920680 MtrunA17_Chr7g0237071 65.698 172 50 2 1 163 36 207 1.05e-73 229
Msa0920680 MtrunA17_Chr4g0008621 39.683 315 144 5 1 299 99 383 1.14e-72 233
Msa0920680 MtrunA17_Chr2g0319041 40.328 305 135 6 1 288 91 365 7.86e-71 232
Msa0920680 MtrunA17_Chr2g0319041 42.424 99 55 2 299 396 460 557 2.05e-17 84.0
Msa0920680 MtrunA17_Chr4g0023911 36.644 292 140 5 11 287 128 389 9.34e-54 187
Msa0920680 MtrunA17_Chr2g0319071 31.567 434 242 14 1 394 69 487 1.23e-53 185
Msa0920680 MtrunA17_Chr2g0323131 35.811 296 143 5 9 287 109 374 5.24e-53 185
Msa0920680 MtrunA17_Chr2g0310331 37.000 300 141 7 16 299 139 406 3.22e-51 181
Msa0920680 MtrunA17_Chr2g0323151 35.473 296 144 5 9 287 25 290 3.38e-51 178
Msa0920680 MtrunA17_Chr6g0466021 71.171 111 26 1 1 105 91 201 5.06e-47 159
Msa0920680 MtrunA17_Chr8g0357411 34.114 299 152 4 16 299 141 409 6.98e-47 169
Msa0920680 MtrunA17_Chr7g0237021 67.347 98 32 0 299 396 75 172 3.16e-44 151
Msa0920680 MtrunA17_Chr7g0236941 67.347 98 32 0 299 396 75 172 3.16e-44 151
Msa0920680 MtrunA17_Chr7g0236921 64.286 98 35 0 299 396 61 158 3.66e-44 150
Msa0920680 MtrunA17_Chr7g0233861 59.231 130 44 2 97 222 33 157 1.23e-43 150
Msa0920680 MtrunA17_Chr6g0465941 62.245 98 37 0 299 396 75 172 4.07e-41 143
Msa0920680 MtrunA17_Chr6g0466121 62.245 98 37 0 299 396 75 172 4.07e-41 143
Msa0920680 MtrunA17_Chr6g0465991 62.245 98 37 0 299 396 75 172 4.07e-41 143
Msa0920680 MtrunA17_Chr6g0466091 62.245 98 37 0 299 396 75 172 4.07e-41 143
Msa0920680 MtrunA17_Chr6g0465881 62.245 98 37 0 299 396 75 172 7.93e-41 142
Msa0920680 MtrunA17_Chr6g0466031 62.245 98 37 0 299 396 163 260 5.99e-40 142
Msa0920680 MtrunA17_Chr6g0466031 46.903 113 30 1 190 302 1 83 1.25e-21 93.6
Msa0920680 MtrunA17_Chr5g0445501 64.286 98 35 0 299 396 75 172 2.11e-39 138
Msa0920680 MtrunA17_Chr7g0236931 48.387 124 62 1 275 396 28 151 4.83e-38 134
Msa0920680 MtrunA17_Chr2g0310401 28.615 325 179 6 37 345 5 292 2.47e-34 132
Msa0920680 MtrunA17_Chr4g0048921 31.469 286 149 7 19 288 126 380 5.76e-32 127
Msa0920680 MtrunA17_Chr4g0048911 28.520 277 150 6 19 280 120 363 7.80e-30 120
Msa0920680 MtrunA17_Chr3g0082711 58.750 80 33 0 317 396 20 99 4.28e-29 108
Msa0920680 MtrunA17_Chr5g0445401 66.292 89 26 2 73 158 7 94 4.33e-29 108
Msa0920680 MtrunA17_Chr2g0280581 27.018 285 162 6 20 288 76 330 2.91e-26 110
Msa0920680 MtrunA17_Chr7g0237061 48.673 113 28 1 190 302 1 83 2.58e-25 103
Msa0920680 MtrunA17_Chr7g0237061 55.556 90 28 1 307 396 160 237 2.87e-24 100
Msa0920680 MtrunA17_Chr6g0477111 27.972 286 158 9 20 288 47 301 4.05e-22 97.8
Msa0920680 MtrunA17_Chr2g0319081 31.788 151 65 2 157 299 138 258 4.67e-21 94.4
Msa0920680 MtrunA17_Chr3g0109021 27.273 286 160 9 20 288 72 326 1.30e-20 93.6
Msa0920680 MtrunA17_Chr3g0105691 25.735 272 157 5 20 276 105 346 1.70e-20 93.6
Msa0920680 MtrunA17_Chr3g0108981 26.667 285 163 7 20 288 48 302 2.37e-20 92.8
Msa0920680 MtrunA17_Chr3g0108961 26.667 285 163 7 20 288 48 302 2.37e-20 92.8
Msa0920680 MtrunA17_Chr8g0357221 37.500 144 83 2 16 152 141 284 2.61e-20 90.5
Msa0920680 MtrunA17_Chr3g0109031 26.573 286 162 9 20 288 72 326 1.33e-19 90.5
Msa0920680 MtrunA17_Chr3g0108941 26.224 286 163 9 20 288 73 327 1.53e-19 90.5
Msa0920680 MtrunA17_Chr3g0109001 26.224 286 163 9 20 288 73 327 1.57e-19 90.5
Msa0920680 MtrunA17_Chr3g0099031 26.224 286 163 9 20 288 72 326 6.07e-19 88.6
Msa0920680 MtrunA17_Chr3g0108991 26.224 286 163 9 20 288 72 326 6.54e-19 88.6
Msa0920680 MtrunA17_Chr3g0108951 26.224 286 163 9 20 288 72 326 6.54e-19 88.6
Msa0920680 MtrunA17_Chr3g0108971 26.224 286 163 9 20 288 47 301 7.64e-19 88.2
Msa0920680 MtrunA17_Chr3g0109011 24.476 286 168 8 20 288 47 301 5.00e-18 85.9
Msa0920680 MtrunA17_Chr6g0477121 26.692 266 147 9 40 288 10 244 9.28e-16 78.6
Msa0920680 MtrunA17_Chr7g0259881 30.108 186 94 6 8 162 267 447 1.77e-13 72.0
Msa0920680 MtrunA17_Chr7g0236981 61.905 42 16 0 1 42 91 132 7.35e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0920680 AT4G20200.1 29.490 451 247 9 17 396 154 604 5.14e-58 199
Msa0920680 AT1G70080.1 34.451 328 152 8 17 312 159 455 1.62e-55 193
Msa0920680 AT3G14490.1 36.395 294 139 5 14 289 148 411 6.62e-55 191
Msa0920680 AT3G14490.1 39.394 99 59 1 299 396 503 601 4.77e-13 71.2
Msa0920680 AT1G31950.2 34.731 334 155 7 14 316 153 454 7.84e-55 189
Msa0920680 AT4G13280.1 27.938 451 220 8 19 396 106 524 2.19e-54 188
Msa0920680 AT1G31950.1 31.951 410 207 10 14 385 152 527 2.74e-54 189
Msa0920680 AT1G31950.3 31.951 410 207 10 14 385 153 528 3.28e-54 189
Msa0920680 AT5G23960.2 36.014 286 137 6 19 289 102 356 4.72e-53 185
Msa0920680 AT5G23960.2 42.857 98 55 1 299 396 449 545 2.05e-19 90.5
Msa0920680 AT5G23960.1 36.364 286 138 6 19 289 102 358 1.26e-52 184
Msa0920680 AT5G23960.1 42.857 98 55 1 299 396 451 547 1.98e-19 90.5
Msa0920680 AT3G14540.2 33.333 336 163 6 11 316 144 448 3.65e-51 179
Msa0920680 AT3G14540.1 33.333 336 163 6 11 316 144 448 1.07e-50 180
Msa0920680 AT3G14520.1 33.660 306 155 4 14 301 150 425 1.07e-50 180
Msa0920680 AT1G33750.1 29.490 451 248 10 15 396 154 603 2.36e-50 179
Msa0920680 AT4G13300.1 27.753 454 251 12 17 396 104 554 2.85e-49 175
Msa0920680 AT4G15870.1 32.573 307 159 6 14 302 157 433 3.51e-48 173
Msa0920680 AT3G29190.1 33.333 303 154 7 17 301 151 423 3.62e-48 173
Msa0920680 AT5G44630.1 32.827 329 161 8 14 312 104 402 8.41e-48 171
Msa0920680 AT5G44630.1 35.000 100 63 1 297 394 456 555 1.12e-11 66.6
Msa0920680 AT3G29410.2 34.247 292 146 6 14 289 47 308 1.06e-46 167
Msa0920680 AT3G29410.2 29.592 98 69 0 299 396 401 498 2.09e-11 65.9
Msa0920680 AT3G29410.1 34.247 292 146 6 14 289 152 413 4.57e-46 167
Msa0920680 AT3G29410.1 29.592 98 69 0 299 396 506 603 3.37e-11 65.5
Msa0920680 AT1G48800.1 27.778 450 249 10 17 392 155 602 4.91e-46 167
Msa0920680 AT1G66020.2 32.558 301 155 6 17 299 148 418 5.66e-46 164
Msa0920680 AT1G66020.1 32.673 303 156 6 17 301 148 420 9.74e-46 166
Msa0920680 AT4G20210.1 31.915 329 163 8 17 315 150 447 1.15e-45 166
Msa0920680 AT4G20210.1 32.653 98 66 0 299 396 503 600 8.02e-11 64.3
Msa0920680 AT4G20230.1 32.119 302 159 6 17 301 154 426 1.11e-43 160
Msa0920680 AT4G20230.2 32.119 302 159 6 17 301 166 438 1.20e-43 160
Msa0920680 AT3G29110.1 32.026 306 162 6 14 302 144 420 1.73e-42 157
Msa0920680 AT3G32030.1 31.126 302 157 5 14 294 151 422 2.57e-42 157
Msa0920680 AT4G16730.2 31.661 319 160 7 6 299 24 309 4.99e-42 154
Msa0920680 AT4G16730.1 31.661 319 160 7 6 299 70 355 7.98e-42 154
Msa0920680 AT4G13280.2 30.233 301 159 6 19 300 106 374 2.73e-39 147
Msa0920680 AT4G13280.2 37.634 93 57 1 305 396 462 554 1.20e-12 69.7
Msa0920680 AT2G23230.2 31.329 316 166 8 6 300 135 420 7.37e-39 147
Msa0920680 AT4G16740.2 30.565 301 152 7 19 299 123 386 4.64e-38 141
Msa0920680 AT2G23230.1 31.111 315 166 8 6 299 135 419 6.06e-38 144
Msa0920680 AT4G16740.1 30.565 301 152 7 19 299 123 386 1.15e-36 140
Msa0920680 AT2G24210.1 30.284 317 161 8 7 299 125 405 5.16e-36 139
Msa0920680 AT1G48820.1 32.443 262 133 6 44 289 156 389 9.72e-34 132
Msa0920680 AT4G16740.4 33.929 224 122 5 19 222 123 340 1.71e-33 129
Msa0920680 AT4G16740.3 33.929 224 122 5 19 222 123 340 1.71e-33 129
Msa0920680 AT3G25820.2 28.975 283 147 8 17 280 140 387 5.69e-33 129
Msa0920680 AT3G25820.1 28.975 283 147 8 17 280 140 387 4.21e-32 128
Msa0920680 AT3G25830.1 28.975 283 147 8 17 280 140 387 4.21e-32 128
Msa0920680 AT3G25820.4 31.111 225 134 6 17 222 140 362 2.86e-31 123
Msa0920680 AT3G25820.3 31.111 225 134 6 17 222 140 362 2.86e-31 123
Msa0920680 AT3G25810.1 29.010 293 150 8 8 275 128 387 5.06e-31 125
Msa0920680 AT5G48110.1 32.143 196 93 4 116 302 1 165 2.50e-26 108
Msa0920680 AT1G61680.1 27.483 302 164 6 4 286 120 385 6.13e-26 110
Msa0920680 AT1G61680.2 26.821 302 159 7 4 286 120 378 7.77e-23 100

Find 57 sgRNAs with CRISPR-Local

Find 337 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTCTTGCTGTGCTATTTA+AGG 0.063679 6_2:-56033535 Msa0920680:CDS
TCTACTTGCGTTGGCAAAAT+TGG 0.132897 6_2:-56032573 Msa0920680:CDS
ACGCAAGTAGAATTTCATTA+TGG 0.236684 6_2:+56032585 None:intergenic
AGCCTACATCAAAAGGAATT+TGG 0.257080 6_2:-56032533 Msa0920680:CDS
TACAAGAGTTATGTTCAAAA+TGG 0.269625 6_2:-56033585 Msa0920680:CDS
GTCATCAATGTATGATAAAA+TGG 0.310334 6_2:+56032270 None:intergenic
GTAGAAATACGCGAAGAAAA+TGG 0.326169 6_2:+56032002 None:intergenic
TAATTGCTTGGAGGATCTTC+CGG 0.335446 6_2:-56031910 Msa0920680:CDS
TGAGGGAATATGATTTGAAT+AGG 0.340079 6_2:-56028351 Msa0920680:CDS
TTCCTTGCTCGTCCTTGAAT+TGG 0.345275 6_2:+56032861 None:intergenic
GAGTAGTAGAATGTTGCTTT+TGG 0.347068 6_2:-56032345 Msa0920680:CDS
CGCAAGTAGAATTTCATTAT+GGG 0.354760 6_2:+56032586 None:intergenic
AGGACATGTTTCTTCGTACT+TGG 0.361077 6_2:-56028383 Msa0920680:CDS
CGTACTTGGAATGTTATATG+AGG 0.388936 6_2:-56028369 Msa0920680:CDS
GGATGAATCTTGCTCATAGA+TGG 0.390050 6_2:+56032607 None:intergenic
TAAAGCAACTTCGTGTAATC+CGG 0.404734 6_2:+56031891 None:intergenic
GTTGCTTTATAAATCACTCT+TGG 0.404999 6_2:-56031877 Msa0920680:CDS
AAAAGAAAATTGACAAAGCT+TGG 0.427323 6_2:-56028306 Msa0920680:CDS
AATAAATAGTCAAATTTCTA+CGG 0.427997 6_2:+56028117 None:intergenic
TTCTGGTCACCTTGAGTCCA+TGG 0.446304 6_2:-56032729 Msa0920680:CDS
GCTCCAAAGCCTACATCAAA+AGG 0.450495 6_2:-56032540 Msa0920680:CDS
TAGGTGGGATATTAATTGCT+TGG 0.452782 6_2:-56031922 Msa0920680:CDS
CAAAAGGAATTTGGAAACAT+TGG 0.456997 6_2:-56032524 Msa0920680:CDS
GATGACACATATGATGCATA+TGG 0.467097 6_2:-56032253 Msa0920680:CDS
GAGCAAGAAATGAAGAAAGA+AGG 0.470516 6_2:-56031837 Msa0920680:CDS
GAGGGAATATGATTTGAATA+GGG 0.475500 6_2:-56028350 Msa0920680:CDS
TTGCAAGAGAAGGACTCAAT+CGG 0.484754 6_2:+56032702 None:intergenic
ATAAATAGTCAAATTTCTAC+GGG 0.486349 6_2:+56028118 None:intergenic
TTGAGAACACACGTAAGAAT+AGG 0.491590 6_2:+56028238 None:intergenic
AGCTTGGAAAGATATAAATC+AGG 0.496605 6_2:-56028290 Msa0920680:CDS
CATGGACTTTACGTTTCACC+TGG 0.512238 6_2:-56033501 Msa0920680:intron
AGGACTCAATCGGGTGGCCA+TGG 0.512884 6_2:+56032712 None:intergenic
AGATGTGTTCAACCAATTCA+AGG 0.513117 6_2:-56032873 Msa0920680:CDS
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6_2:+56032635 None:intergenic
GCCTCACATATGATGGTTCA+TGG 0.522278 6_2:-56032785 Msa0920680:intron
ATATAGGTGGTGGAAGGAGT+TGG 0.524102 6_2:-56032401 Msa0920680:intron
GTGGGATATTAATTGCTTGG+AGG 0.529634 6_2:-56031919 Msa0920680:CDS
ATAGGTAACGGAACTTCAGT+TGG 0.529746 6_2:+56028256 None:intergenic
TAATGAAATTCTACTTGCGT+TGG 0.533622 6_2:-56032582 Msa0920680:CDS
CTGTGCTATTTAAGGTGTTG+AGG 0.537954 6_2:-56033527 Msa0920680:CDS
AACATTAGTCAGAGATGTTG+AGG 0.538556 6_2:-56032828 Msa0920680:CDS
AAACAGTTTGAGCAGAAAAG+AGG 0.539017 6_2:-56028403 Msa0920680:intron
TGCAAGAGAAGGACTCAATC+GGG 0.546913 6_2:+56032703 None:intergenic
TTTACTTGTGTTGCAAGAGA+AGG 0.558925 6_2:+56032692 None:intergenic
TTTAAGGTGTTGAGGCAACA+TGG 0.562250 6_2:-56033519 Msa0920680:CDS
ATCGGGTGGCCATGGACTCA+AGG 0.566605 6_2:+56032720 None:intergenic
GTACTTGGAATGTTATATGA+GGG 0.567173 6_2:-56028368 Msa0920680:CDS
GTATGAAGCCTCACATATGA+TGG 0.567934 6_2:-56032792 Msa0920680:CDS
CTTTATATATAGGTGGTGGA+AGG 0.588928 6_2:-56032407 Msa0920680:intron
ACTACTCTATCTCGTGCATA+CGG 0.590301 6_2:+56032361 None:intergenic
ATAGCACAGCAAGAGAATGA+AGG 0.592769 6_2:+56033541 None:intergenic
ACATTAGTCAGAGATGTTGA+GGG 0.599562 6_2:-56032827 Msa0920680:CDS
GATAACTACACACACTCTGA+CGG 0.610210 6_2:-56028178 Msa0920680:CDS
AACCAATTCAAGGACGAGCA+AGG 0.615624 6_2:-56032863 Msa0920680:CDS
ACCATGAACCATCATATGTG+AGG 0.634158 6_2:+56032784 None:intergenic
ACACACGTAAGAATAGGTAA+CGG 0.686084 6_2:+56028244 None:intergenic
AAGAGAAGGACTCAATCGGG+TGG 0.695745 6_2:+56032706 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTATGTTTTTAATT+TGG - chr6_2:56028276-56028295 Msa0920680:CDS 10.0%
!!! TATTTTTTGTCTTTATATAT+AGG - chr6_2:56029306-56029325 Msa0920680:intron 10.0%
!! AATATAAAATAAACGTTAAT+AGG + chr6_2:56030652-56030671 None:intergenic 10.0%
!! TAATATGAATGTCTAAAAAA+TGG - chr6_2:56028408-56028427 Msa0920680:intron 15.0%
!!! ATTGTTGTTTAATATTATTG+TGG - chr6_2:56028809-56028828 Msa0920680:intron 15.0%
!!! ATACTTTTATATTTTGATGT+AGG + chr6_2:56029244-56029263 None:intergenic 15.0%
!! ATAAAGACAAAAAATAGTAT+AGG + chr6_2:56029302-56029321 None:intergenic 15.0%
!!! ATTTCATTTTAAACTTTGTA+TGG - chr6_2:56030045-56030064 Msa0920680:intron 15.0%
!!! AAACATTTTTACATCATTTT+AGG - chr6_2:56030146-56030165 Msa0920680:intron 15.0%
!! TTAACAACATTTAATTACAT+AGG - chr6_2:56030610-56030629 Msa0920680:intron 15.0%
!!! TTTAATTACATAGGTTTTTT+TGG - chr6_2:56030619-56030638 Msa0920680:intron 15.0%
!! AGATAAATTAGATATATCAA+GGG + chr6_2:56033278-56033297 None:intergenic 15.0%
!! TAGATAAATTAGATATATCA+AGG + chr6_2:56033279-56033298 None:intergenic 15.0%
!!! CTTTTTACTAAAAGATTTAC+AGG + chr6_2:56028448-56028467 None:intergenic 20.0%
!! GAGAATAATTAAAAGAAATG+AGG + chr6_2:56028584-56028603 None:intergenic 20.0%
!! AATATTAATGGTATACATGT+AGG - chr6_2:56028657-56028676 Msa0920680:intron 20.0%
!!! TTTTTGTCTTTATATATAGG+TGG - chr6_2:56029309-56029328 Msa0920680:intron 20.0%
!!! AGATAAATTTTGACATCATA+CGG + chr6_2:56029433-56029452 None:intergenic 20.0%
!!! ATTGTTTTATCTCTAAAGTT+TGG + chr6_2:56029585-56029604 None:intergenic 20.0%
!!! TTGCAATCAAAATGATTTTT+AGG - chr6_2:56029782-56029801 Msa0920680:intron 20.0%
!!! AATCAAAATGATTTTTAGGT+GGG - chr6_2:56029786-56029805 Msa0920680:intron 20.0%
!!! TCATGAAGATATATTTTTCT+TGG + chr6_2:56030207-56030226 None:intergenic 20.0%
!! TGTAAAAAACTTATTATCCA+TGG - chr6_2:56030285-56030304 Msa0920680:intron 20.0%
!!! TATCTTGACATTTTAATTAG+TGG - chr6_2:56030553-56030572 Msa0920680:intron 20.0%
!! AATATTATGATACTGATAAG+CGG + chr6_2:56030821-56030840 None:intergenic 20.0%
!!! AAAGGATATTTATTGGATTT+TGG - chr6_2:56030985-56031004 Msa0920680:intron 20.0%
!! ACATGAATTATGTTAAGATT+AGG - chr6_2:56031131-56031150 Msa0920680:intron 20.0%
!! ACATTATAAAACATCTTTCA+TGG + chr6_2:56031212-56031231 None:intergenic 20.0%
!! TATAATGTTATATAAGTTGC+TGG - chr6_2:56031224-56031243 Msa0920680:intron 20.0%
! TACAAGAGTTATGTTCAAAA+TGG - chr6_2:56028138-56028157 Msa0920680:CDS 25.0%
!! AATAAGTTGAGATATATACC+AGG + chr6_2:56028243-56028262 None:intergenic 25.0%
! TTGAGTAAAACAAATTAGAG+TGG - chr6_2:56028307-56028326 Msa0920680:CDS 25.0%
!! GTAATATTTGACTTTGTCAT+GGG - chr6_2:56028379-56028398 Msa0920680:CDS 25.0%
! GTCATCAATGTATGATAAAA+TGG + chr6_2:56029456-56029475 None:intergenic 25.0%
!! TGAATTAGAACTTTTTACTC+AGG - chr6_2:56029502-56029521 Msa0920680:intron 25.0%
!! AATTTACGCATTTTCAATCT+TGG - chr6_2:56029667-56029686 Msa0920680:intron 25.0%
!! TTCTTTTAACAATAGAGCAA+TGG - chr6_2:56029753-56029772 Msa0920680:intron 25.0%
!!! CAATCAAAATGATTTTTAGG+TGG - chr6_2:56029785-56029804 Msa0920680:intron 25.0%
!!! ACTCTTACTTCTTTTAAGAA+GGG + chr6_2:56029931-56029950 None:intergenic 25.0%
!!! AACTCTTACTTCTTTTAAGA+AGG + chr6_2:56029932-56029951 None:intergenic 25.0%
! ATGAAATCTTTGTTCAATTG+TGG + chr6_2:56030032-56030051 None:intergenic 25.0%
! AAACTTTGTATGGTTATCAA+AGG - chr6_2:56030055-56030074 Msa0920680:intron 25.0%
! AACTTTGTATGGTTATCAAA+GGG - chr6_2:56030056-56030075 Msa0920680:intron 25.0%
!!! TATCCATGGTTTTATTAACT+CGG - chr6_2:56030299-56030318 Msa0920680:intron 25.0%
! AAGAAAAATGTATCATCATG+AGG - chr6_2:56030361-56030380 Msa0920680:intron 25.0%
! TGCAAAAAACTTATTATCGA+TGG - chr6_2:56030441-56030460 Msa0920680:intron 25.0%
! ATATTATGATACTGATAAGC+GGG + chr6_2:56030820-56030839 None:intergenic 25.0%
!! GTTTATGAAAAGTAAGTTGA+CGG - chr6_2:56030868-56030887 Msa0920680:intron 25.0%
!! AAATGGGAAATGAGATATTT+TGG + chr6_2:56031013-56031032 None:intergenic 25.0%
! GATTCTTGAAATGAAAATCA+TGG - chr6_2:56031539-56031558 Msa0920680:intron 25.0%
! TTAGTAAAGAATAGAATGCT+AGG - chr6_2:56031682-56031701 Msa0920680:intron 25.0%
!! CATTATCTGAGTTCATTTTA+CGG + chr6_2:56032224-56032243 None:intergenic 25.0%
!! AAACATCGTATTTTGCATTA+GGG - chr6_2:56032436-56032455 Msa0920680:intron 25.0%
! GTCATGATTATGTTAGAATT+AGG - chr6_2:56032522-56032541 Msa0920680:CDS 25.0%
!! ATCTGAAACAAATAAGGATT+GGG + chr6_2:56032808-56032827 None:intergenic 25.0%
!! TATCTGAAACAAATAAGGAT+TGG + chr6_2:56032809-56032828 None:intergenic 25.0%
!! CTGTTTATCTGAAACAAATA+AGG + chr6_2:56032814-56032833 None:intergenic 25.0%
! CTTATTTGTTTCAGATAAAC+AGG - chr6_2:56032812-56032831 Msa0920680:CDS 25.0%
!!! AAGCTTTGTCAATTTTCTTT+TGG + chr6_2:56033418-56033437 None:intergenic 25.0%
! AAAAGAAAATTGACAAAGCT+TGG - chr6_2:56033417-56033436 Msa0920680:intron 25.0%
! CGTAATATTTGACTTTGTCA+TGG - chr6_2:56028378-56028397 Msa0920680:CDS 30.0%
ATGTGAACATTACACAGATA+AGG + chr6_2:56028561-56028580 None:intergenic 30.0%
!! TGTTTCTGAAGTACGAAAAA+GGG + chr6_2:56028699-56028718 None:intergenic 30.0%
GGTTCATGGTGAAAAAATAT+AGG - chr6_2:56028952-56028971 Msa0920680:intron 30.0%
TGGTGAAAAAATATAGGAAG+AGG - chr6_2:56028958-56028977 Msa0920680:intron 30.0%
CGCAAGTAGAATTTCATTAT+GGG + chr6_2:56029140-56029159 None:intergenic 30.0%
ACGCAAGTAGAATTTCATTA+TGG + chr6_2:56029141-56029160 None:intergenic 30.0%
TAATGAAATTCTACTTGCGT+TGG - chr6_2:56029141-56029160 Msa0920680:intron 30.0%
! TTCCAAATTCCTTTTGATGT+AGG + chr6_2:56029195-56029214 None:intergenic 30.0%
CAAAAGGAATTTGGAAACAT+TGG - chr6_2:56029199-56029218 Msa0920680:intron 30.0%
TTGTCTTTATATATAGGTGG+TGG - chr6_2:56029312-56029331 Msa0920680:intron 30.0%
!!! AGTAGTAGAATGTTGCTTTT+GGG - chr6_2:56029379-56029398 Msa0920680:intron 30.0%
GTTATGACCGTATAATCTTA+GGG - chr6_2:56029401-56029420 Msa0920680:intron 30.0%
GTAGTTTCCCTAAGATTATA+CGG + chr6_2:56029411-56029430 None:intergenic 30.0%
! TTTTTACTCAGGCAATTGAA+AGG - chr6_2:56029513-56029532 Msa0920680:intron 30.0%
! GTTGCTTTATAAATCACTCT+TGG - chr6_2:56029846-56029865 Msa0920680:intron 30.0%
!! CTCTTACTTCTTTTAAGAAG+GGG + chr6_2:56029930-56029949 None:intergenic 30.0%
! AAAGTATCACCATAATGACT+TGG - chr6_2:56030181-56030200 Msa0920680:intron 30.0%
TAACTATATACCAACAACAG+AGG - chr6_2:56030737-56030756 Msa0920680:intron 30.0%
CACATATCAACAATATCAAG+TGG - chr6_2:56030768-56030787 Msa0920680:intron 30.0%
TTTCATAAACTTTCTTCCTC+TGG + chr6_2:56030858-56030877 None:intergenic 30.0%
GCACGAAAAAGGATATTTAT+TGG - chr6_2:56030978-56030997 Msa0920680:intron 30.0%
TGGCAATATAAACATGTACA+AGG + chr6_2:56031192-56031211 None:intergenic 30.0%
! GATCAAATTGGACAAGTTTT+GGG - chr6_2:56031267-56031286 Msa0920680:intron 30.0%
AAACATCGAAAGAAAAGCTT+CGG + chr6_2:56031467-56031486 None:intergenic 30.0%
CATGGAAATCTTAACATGAA+TGG - chr6_2:56031557-56031576 Msa0920680:intron 30.0%
! AAATGCTATCTCTTTTACCT+CGG + chr6_2:56031872-56031891 None:intergenic 30.0%
!!! GACCAAGACTTTTAAGTTAA+AGG - chr6_2:56031920-56031939 Msa0920680:CDS 30.0%
! CACCTTTAACTTAAAAGTCT+TGG + chr6_2:56031925-56031944 None:intergenic 30.0%
! GAAACATCGTATTTTGCATT+AGG - chr6_2:56032435-56032454 Msa0920680:intron 30.0%
! GTGTCTAAAATACAACATGA+CGG + chr6_2:56032709-56032728 None:intergenic 30.0%
TTTGTTTCAGATAAACAGGT+TGG - chr6_2:56032816-56032835 Msa0920680:CDS 30.0%
TTATTATGTACGAACGCATT+TGG - chr6_2:56032951-56032970 Msa0920680:intron 30.0%
! CATGTGTATTAGTTTAGTAC+TGG + chr6_2:56033026-56033045 None:intergenic 30.0%
TTTCTAGATACGTCTAGTAT+GGG - chr6_2:56033052-56033071 Msa0920680:intron 30.0%
TATGACTTCTTACATTGGAA+TGG - chr6_2:56033084-56033103 Msa0920680:intron 30.0%
ATGACTTCTTACATTGGAAT+GGG - chr6_2:56033085-56033104 Msa0920680:intron 30.0%
GTACTTGGAATGTTATATGA+GGG - chr6_2:56033355-56033374 Msa0920680:intron 30.0%
TGAGGGAATATGATTTGAAT+AGG - chr6_2:56033372-56033391 Msa0920680:intron 30.0%
GAGGGAATATGATTTGAATA+GGG - chr6_2:56033373-56033392 Msa0920680:intron 30.0%
! TTTCTTTTGGCATTCTTGAA+TGG + chr6_2:56033405-56033424 None:intergenic 30.0%
AGCTTGGAAAGATATAAATC+AGG - chr6_2:56033433-56033452 Msa0920680:intron 30.0%
!! TTCTCTTGCTGTGCTATTTA+AGG - chr6_2:56028188-56028207 Msa0920680:CDS 35.0%
AAAGATTTACAGGCTAACAG+TGG + chr6_2:56028438-56028457 None:intergenic 35.0%
!! GTGTTTCTGAAGTACGAAAA+AGG + chr6_2:56028700-56028719 None:intergenic 35.0%
AGATGTGTTCAACCAATTCA+AGG - chr6_2:56028850-56028869 Msa0920680:intron 35.0%
AACATTAGTCAGAGATGTTG+AGG - chr6_2:56028895-56028914 Msa0920680:intron 35.0%
ACATTAGTCAGAGATGTTGA+GGG - chr6_2:56028896-56028915 Msa0920680:intron 35.0%
! AAAAATATAGGAAGAGGCGT+TGG - chr6_2:56028964-56028983 Msa0920680:intron 35.0%
TTTACTTGTGTTGCAAGAGA+AGG + chr6_2:56029034-56029053 None:intergenic 35.0%
! ATTCCTTTTGATGTAGGCTT+TGG + chr6_2:56029189-56029208 None:intergenic 35.0%
AGCCTACATCAAAAGGAATT+TGG - chr6_2:56029190-56029209 Msa0920680:intron 35.0%
!! CTTTATATATAGGTGGTGGA+AGG - chr6_2:56029316-56029335 Msa0920680:intron 35.0%
!!! GAGTAGTAGAATGTTGCTTT+TGG - chr6_2:56029378-56029397 Msa0920680:intron 35.0%
GGTTATGACCGTATAATCTT+AGG - chr6_2:56029400-56029419 Msa0920680:intron 35.0%
GATGACACATATGATGCATA+TGG - chr6_2:56029470-56029489 Msa0920680:intron 35.0%
! CTAGTCATCAAGTTTAGAGT+TGG - chr6_2:56029623-56029642 Msa0920680:intron 35.0%
GTAGAAATACGCGAAGAAAA+TGG + chr6_2:56029724-56029743 None:intergenic 35.0%
TAGGTGGGATATTAATTGCT+TGG - chr6_2:56029801-56029820 Msa0920680:intron 35.0%
TAAAGCAACTTCGTGTAATC+CGG + chr6_2:56029835-56029854 None:intergenic 35.0%
GAGCAAGAAATGAAGAAAGA+AGG - chr6_2:56029886-56029905 Msa0920680:intron 35.0%
!! TTCTTTTAAGAAGGGGTCAA+GGG + chr6_2:56029923-56029942 None:intergenic 35.0%
TGGTTATCAAAGGGAAGTTT+AGG - chr6_2:56030065-56030084 Msa0920680:intron 35.0%
! TTTTCTTGGCCAAGTCATTA+TGG + chr6_2:56030193-56030212 None:intergenic 35.0%
GAACCGAGTTAATAAAACCA+TGG + chr6_2:56030305-56030324 None:intergenic 35.0%
AATCCAAGCTTATATGAGTG+AGG - chr6_2:56030698-56030717 Msa0920680:intron 35.0%
ATATTACAGTAGCCAACACT+AGG + chr6_2:56030797-56030816 None:intergenic 35.0%
TACAAGTTAAGCATCAGTGT+CGG - chr6_2:56030919-56030938 Msa0920680:intron 35.0%
TTCTGGTAATTGCACGAAAA+AGG - chr6_2:56030967-56030986 Msa0920680:intron 35.0%
! GGATCAAATTGGACAAGTTT+TGG - chr6_2:56031266-56031285 Msa0920680:intron 35.0%
TTAACCCCAAAACAACATCT+AGG - chr6_2:56031496-56031515 Msa0920680:intron 35.0%
GTTATTGACAAGCTATGTCA+AGG + chr6_2:56031654-56031673 None:intergenic 35.0%
ATGTCTAATCAATTCACGTC+TGG + chr6_2:56031789-56031808 None:intergenic 35.0%
GCATATAGTTTACCTCTAGT+CGG - chr6_2:56031833-56031852 Msa0920680:CDS 35.0%
! CCTATCTAAGGTATAACCAA+TGG + chr6_2:56032149-56032168 None:intergenic 35.0%
! CCATTGGTTATACCTTAGAT+AGG - chr6_2:56032146-56032165 Msa0920680:intron 35.0%
TACCTTAGATAGGATTCCAT+CGG - chr6_2:56032156-56032175 Msa0920680:intron 35.0%
ATCATGACACGATTCTAAAC+CGG + chr6_2:56032510-56032529 None:intergenic 35.0%
CAAGTGAGATAAGACTATGA+CGG - chr6_2:56032630-56032649 Msa0920680:CDS 35.0%
!! CATGTTGTATTTTAGACACG+TGG - chr6_2:56032710-56032729 Msa0920680:CDS 35.0%
CTTTCTAGATACGTCTAGTA+TGG - chr6_2:56033051-56033070 Msa0920680:intron 35.0%
ACAGCTATGACTTCTTACAT+TGG - chr6_2:56033079-56033098 Msa0920680:intron 35.0%
GACAAAAGACATCTTTGACT+GGG - chr6_2:56033117-56033136 Msa0920680:intron 35.0%
! GCAGCAGCATTAACAATTTT+TGG + chr6_2:56033154-56033173 None:intergenic 35.0%
AAACAGTTTGAGCAGAAAAG+AGG - chr6_2:56033320-56033339 Msa0920680:intron 35.0%
CGTACTTGGAATGTTATATG+AGG - chr6_2:56033354-56033373 Msa0920680:intron 35.0%
ACACACGTAAGAATAGGTAA+CGG + chr6_2:56033482-56033501 None:intergenic 35.0%
TTGAGAACACACGTAAGAAT+AGG + chr6_2:56033488-56033507 None:intergenic 35.0%
ATAGCACAGCAAGAGAATGA+AGG + chr6_2:56028185-56028204 None:intergenic 40.0%
!! CTGTGCTATTTAAGGTGTTG+AGG - chr6_2:56028196-56028215 Msa0920680:CDS 40.0%
!! TTTAAGGTGTTGAGGCAACA+TGG - chr6_2:56028204-56028223 Msa0920680:CDS 40.0%
!! AGGAGTGTCTTTCGTTTGAT+CGG - chr6_2:56028518-56028537 Msa0920680:intron 40.0%
GTATGAAGCCTCACATATGA+TGG - chr6_2:56028931-56028950 Msa0920680:intron 40.0%
ACCATGAACCATCATATGTG+AGG + chr6_2:56028942-56028961 None:intergenic 40.0%
TTGCAAGAGAAGGACTCAAT+CGG + chr6_2:56029024-56029043 None:intergenic 40.0%
GGATGAATCTTGCTCATAGA+TGG + chr6_2:56029119-56029138 None:intergenic 40.0%
! TCTACTTGCGTTGGCAAAAT+TGG - chr6_2:56029150-56029169 Msa0920680:intron 40.0%
ACTACTCTATCTCGTGCATA+CGG + chr6_2:56029365-56029384 None:intergenic 40.0%
GTGGGATATTAATTGCTTGG+AGG - chr6_2:56029804-56029823 Msa0920680:intron 40.0%
TAATTGCTTGGAGGATCTTC+CGG - chr6_2:56029813-56029832 Msa0920680:intron 40.0%
!! CTTCTTTTAAGAAGGGGTCA+AGG + chr6_2:56029924-56029943 None:intergenic 40.0%
ATAGTTAGCATTCAGCCATC+TGG + chr6_2:56030724-56030743 None:intergenic 40.0%
ATCAAGTGGTTACCTAGTGT+TGG - chr6_2:56030782-56030801 Msa0920680:intron 40.0%
! ATTTAACACGTCTGAGGCAA+GGG - chr6_2:56030942-56030961 Msa0920680:intron 40.0%
CCTAGAGAGAGAAGTGAAAT+GGG + chr6_2:56031029-56031048 None:intergenic 40.0%
ACCTAGAGAGAGAAGTGAAA+TGG + chr6_2:56031030-56031049 None:intergenic 40.0%
! CACTTCTCTCTCTAGGTTTT+TGG - chr6_2:56031033-56031052 Msa0920680:intron 40.0%
ACATGTACAAGGCAAGATCT+AGG + chr6_2:56031181-56031200 None:intergenic 40.0%
AGTGCATTGCAGGATCAAAT+TGG - chr6_2:56031255-56031274 Msa0920680:intron 40.0%
!!! GACAAGTTTTGGGTCAAAAC+TGG - chr6_2:56031277-56031296 Msa0920680:intron 40.0%
CGCTAATAGCGAAATTCTTC+AGG - chr6_2:56031347-56031366 Msa0920680:intron 40.0%
!!! GTTGCCTAGATGTTGTTTTG+GGG + chr6_2:56031503-56031522 None:intergenic 40.0%
!!! CGTTGCCTAGATGTTGTTTT+GGG + chr6_2:56031504-56031523 None:intergenic 40.0%
! GAGAGATTAGCCGAATTACT+CGG - chr6_2:56031594-56031613 Msa0920680:intron 40.0%
! AGAGATTAGCCGAATTACTC+GGG - chr6_2:56031595-56031614 Msa0920680:intron 40.0%
TCTTTGCTACCCGAGTAATT+CGG + chr6_2:56031607-56031626 None:intergenic 40.0%
ACTTAGTAAGCAGGATGTAG+CGG - chr6_2:56032028-56032047 Msa0920680:intron 40.0%
ATCCGATGGAATCCTATCTA+AGG + chr6_2:56032161-56032180 None:intergenic 40.0%
TAGGATTCCATCGGATAGAT+AGG - chr6_2:56032165-56032184 Msa0920680:intron 40.0%
AGGATTCCATCGGATAGATA+GGG - chr6_2:56032166-56032185 Msa0920680:intron 40.0%
ATTCTTGACGATCTACGTCA+AGG + chr6_2:56032324-56032343 None:intergenic 40.0%
ATTCTTGACGATCTACGTCA+AGG + chr6_2:56032324-56032343 None:intergenic 40.0%
GATATGCCGATGATGATCTT+AGG - chr6_2:56032471-56032490 Msa0920680:intron 40.0%
GGGAAACCTAAGATCATCAT+CGG + chr6_2:56032480-56032499 None:intergenic 40.0%
GATTCTAAACCGGACGATTA+GGG + chr6_2:56032500-56032519 None:intergenic 40.0%
AGAATACGCTCTACGTATTG+AGG + chr6_2:56032548-56032567 None:intergenic 40.0%
CAATACGTAGAGCGTATTCT+TGG - chr6_2:56032548-56032567 Msa0920680:CDS 40.0%
TTCACTTCCTATACGTGTCA+TGG + chr6_2:56032602-56032621 None:intergenic 40.0%
ACGGTTGATTATACGTGTGT+GGG + chr6_2:56032690-56032709 None:intergenic 40.0%
TTGCCTATAGCCTAGGATTT+AGG - chr6_2:56032755-56032774 Msa0920680:intron 40.0%
TGCCTATAGCCTAGGATTTA+GGG - chr6_2:56032756-56032775 Msa0920680:intron 40.0%
CGACAAAAGACATCTTTGAC+TGG - chr6_2:56033116-56033135 Msa0920680:intron 40.0%
TGCTATACATTGCAGGCTTA+GGG - chr6_2:56033171-56033190 Msa0920680:intron 40.0%
AGGACATGTTTCTTCGTACT+TGG - chr6_2:56033340-56033359 Msa0920680:intron 40.0%
!! ATAGGTAACGGAACTTCAGT+TGG + chr6_2:56033470-56033489 None:intergenic 40.0%
!! CAATCTCTCGCGTTTTATTG+AGG - chr6_2:56033505-56033524 Msa0920680:CDS 40.0%
GATAACTACACACACTCTGA+CGG - chr6_2:56033545-56033564 Msa0920680:CDS 40.0%
CATGGACTTTACGTTTCACC+TGG - chr6_2:56028222-56028241 Msa0920680:CDS 45.0%
AACCAATTCAAGGACGAGCA+AGG - chr6_2:56028860-56028879 Msa0920680:intron 45.0%
TTCCTTGCTCGTCCTTGAAT+TGG + chr6_2:56028865-56028884 None:intergenic 45.0%
GCCTCACATATGATGGTTCA+TGG - chr6_2:56028938-56028957 Msa0920680:intron 45.0%
TGCAAGAGAAGGACTCAATC+GGG + chr6_2:56029023-56029042 None:intergenic 45.0%
CAAAAACTTGCCTAGGCTAG+TGG - chr6_2:56029078-56029097 Msa0920680:intron 45.0%
! TAACTTCTTGCCACTAGCCT+AGG + chr6_2:56029091-56029110 None:intergenic 45.0%
GCTCCAAAGCCTACATCAAA+AGG - chr6_2:56029183-56029202 Msa0920680:intron 45.0%
!! ATATAGGTGGTGGAAGGAGT+TGG - chr6_2:56029322-56029341 Msa0920680:intron 45.0%
TGGCCTCACTCATATAAGCT+TGG + chr6_2:56030704-56030723 None:intergenic 45.0%
CTTATATGAGTGAGGCCAGA+TGG - chr6_2:56030706-56030725 Msa0920680:intron 45.0%
TGCATGTACTCCTCTGTTGT+TGG + chr6_2:56030750-56030769 None:intergenic 45.0%
TGTCGGATTTAACACGTCTG+AGG - chr6_2:56030936-56030955 Msa0920680:intron 45.0%
! GATTTAACACGTCTGAGGCA+AGG - chr6_2:56030941-56030960 Msa0920680:intron 45.0%
CCCATTTCACTTCTCTCTCT+AGG - chr6_2:56031026-56031045 Msa0920680:intron 45.0%
TTTCTCTCCTCCTTCTCTCT+AGG - chr6_2:56031064-56031083 Msa0920680:intron 45.0%
TAGAAGAAGGAAGAATGGGG+CGG + chr6_2:56031090-56031109 None:intergenic 45.0%
CCCTAGAAGAAGGAAGAATG+GGG + chr6_2:56031093-56031112 None:intergenic 45.0%
CCCCATTCTTCCTTCTTCTA+GGG - chr6_2:56031090-56031109 Msa0920680:intron 45.0%
GCCCTAGAAGAAGGAAGAAT+GGG + chr6_2:56031094-56031113 None:intergenic 45.0%
TGCCCTAGAAGAAGGAAGAA+TGG + chr6_2:56031095-56031114 None:intergenic 45.0%
! AGCTTTGATGCCCTAGAAGA+AGG + chr6_2:56031103-56031122 None:intergenic 45.0%
ACAAGGCAAGATCTAGGCAA+GGG + chr6_2:56031175-56031194 None:intergenic 45.0%
TACAAGGCAAGATCTAGGCA+AGG + chr6_2:56031176-56031195 None:intergenic 45.0%
GGATGTTCATAGTGCATTGC+AGG - chr6_2:56031245-56031264 Msa0920680:intron 45.0%
TCGCTAATAGCGTGGTTCTT+CGG + chr6_2:56031321-56031340 None:intergenic 45.0%
CGTAGATTTCGCTAATAGCG+TGG + chr6_2:56031329-56031348 None:intergenic 45.0%
AAAAGCTTCGGAATGCACCA+CGG + chr6_2:56031455-56031474 None:intergenic 45.0%
!! GCGTTGCCTAGATGTTGTTT+TGG + chr6_2:56031505-56031524 None:intergenic 45.0%
TACTCGGGTAGCAAAGAACT+AGG - chr6_2:56031610-56031629 Msa0920680:intron 45.0%
GGTAGCAAAGAACTAGGCAT+GGG - chr6_2:56031616-56031635 Msa0920680:intron 45.0%
TACCTCTAGTCGGATCGTAT+AGG - chr6_2:56031843-56031862 Msa0920680:CDS 45.0%
TAGTGTGGACACAAGAGACA+TGG + chr6_2:56031948-56031967 None:intergenic 45.0%
ATGTCTCTTGTGTCCACACT+AGG - chr6_2:56031947-56031966 Msa0920680:CDS 45.0%
GGGGTTTCCATTAGGTGAAA+CGG - chr6_2:56031979-56031998 Msa0920680:CDS 45.0%
GGGTTTCCATTAGGTGAAAC+GGG - chr6_2:56031980-56031999 Msa0920680:CDS 45.0%
GGTTTCCATTAGGTGAAACG+GGG - chr6_2:56031981-56032000 Msa0920680:CDS 45.0%
CTGCTTACTAAGTGACATGC+GGG + chr6_2:56032021-56032040 None:intergenic 45.0%
CCTGCTTACTAAGTGACATG+CGG + chr6_2:56032022-56032041 None:intergenic 45.0%
!! TCCACACTAGGTTGGTTCAA+GGG - chr6_2:56032065-56032084 Msa0920680:intron 45.0%
TGGTTCAAGGGGTTTCCATT+AGG - chr6_2:56032077-56032096 Msa0920680:intron 45.0%
GGGGTTTCCATTAGGAGAAA+CGG - chr6_2:56032085-56032104 Msa0920680:intron 45.0%
GGGTTTCCATTAGGAGAAAC+GGG - chr6_2:56032086-56032105 Msa0920680:intron 45.0%
GGTTTCCATTAGGAGAAACG+GGG - chr6_2:56032087-56032106 Msa0920680:intron 45.0%
CTTAGGTTTCCCTAATCGTC+CGG - chr6_2:56032488-56032507 Msa0920680:intron 45.0%
CGATTCTAAACCGGACGATT+AGG + chr6_2:56032501-56032520 None:intergenic 45.0%
AGAGCGTATTCTTGGCATTG+CGG - chr6_2:56032556-56032575 Msa0920680:CDS 45.0%
ACTTCCTATACGTGTCATGG+CGG + chr6_2:56032599-56032618 None:intergenic 45.0%
TTAGACGGTCGTGCATACTA+GGG + chr6_2:56032656-56032675 None:intergenic 45.0%
TGGGGACTTGCATACTTAGA+CGG + chr6_2:56032671-56032690 None:intergenic 45.0%
CGGTTGATTATACGTGTGTG+GGG + chr6_2:56032689-56032708 None:intergenic 45.0%
GACGGTTGATTATACGTGTG+TGG + chr6_2:56032691-56032710 None:intergenic 45.0%
GCCTATAGCCTAGGATTTAG+GGG - chr6_2:56032757-56032776 Msa0920680:intron 45.0%
TCCCCTAAATCCTAGGCTAT+AGG + chr6_2:56032761-56032780 None:intergenic 45.0%
AGTGAGTTCCCCTAAATCCT+AGG + chr6_2:56032768-56032787 None:intergenic 45.0%
TTAGCAAGCTAGTAGCCGAA+TGG - chr6_2:56032840-56032859 Msa0920680:CDS 45.0%
CACTTGTCGCATCATCCATT+CGG + chr6_2:56032858-56032877 None:intergenic 45.0%
CAGGACGATGTGGATATTCT+AGG - chr6_2:56032894-56032913 Msa0920680:intron 45.0%
ATGCTGCTGCTATACATTGC+AGG - chr6_2:56033164-56033183 Msa0920680:intron 45.0%
CTGCTATACATTGCAGGCTT+AGG - chr6_2:56033170-56033189 Msa0920680:intron 45.0%
GGATGAAATTGTGTCAAGCG+AGG - chr6_2:56033192-56033211 Msa0920680:intron 45.0%
!!! AATTTTTTTTTACATAAAAT+AGG - chr6_2:56028498-56028517 Msa0920680:intron 5.0%
!! TTATTATAAAGTAATATTAA+TGG - chr6_2:56028645-56028664 Msa0920680:intron 5.0%
!!! GAGGCGTTGGCTTTTACTTC+TGG - chr6_2:56028977-56028996 Msa0920680:intron 50.0%
TTCTGGTCACCTTGAGTCCA+TGG - chr6_2:56028994-56029013 Msa0920680:intron 50.0%
AAGAGAAGGACTCAATCGGG+TGG + chr6_2:56029020-56029039 None:intergenic 50.0%
CTCTGCGCAAAAACTTGCCT+AGG - chr6_2:56029071-56029090 Msa0920680:intron 50.0%
TTATCAGTCAAGCGTGCCAG+AGG - chr6_2:56030839-56030858 Msa0920680:intron 50.0%
GCCCCATTCTTCCTTCTTCT+AGG - chr6_2:56031089-56031108 Msa0920680:intron 50.0%
CAAGGCAAGATCTAGGCAAG+GGG + chr6_2:56031174-56031193 None:intergenic 50.0%
CGCTAATAGCGTGGTTCTTC+GGG + chr6_2:56031320-56031339 None:intergenic 50.0%
! TGGACATGGCCAACTTGCTT+TGG - chr6_2:56031408-56031427 Msa0920680:intron 50.0%
GGGTAGCAAAGAACTAGGCA+TGG - chr6_2:56031615-56031634 Msa0920680:intron 50.0%
! ACTAGAGTGCCATACGAGTG+TGG - chr6_2:56031763-56031782 Msa0920680:intron 50.0%
TGCCTATACGATCCGACTAG+AGG + chr6_2:56031848-56031867 None:intergenic 50.0%
CTCTTGTGTCCACACTAGGT+TGG - chr6_2:56031951-56031970 Msa0920680:CDS 50.0%
! TCCACACTAGGTTGGCTCAA+GGG - chr6_2:56031959-56031978 Msa0920680:CDS 50.0%
TGGCTCAAGGGGTTTCCATT+AGG - chr6_2:56031971-56031990 Msa0920680:CDS 50.0%
GCTAACCCCGTTTCACCTAA+TGG + chr6_2:56031989-56032008 None:intergenic 50.0%
AGTGACATGCGGGTTCCTTA+CGG + chr6_2:56032011-56032030 None:intergenic 50.0%
! CCGCATGTCACTTAGTAAGC+AGG - chr6_2:56032019-56032038 Msa0920680:intron 50.0%
GTGTCTCTTGTGTCCACACT+AGG - chr6_2:56032053-56032072 Msa0920680:intron 50.0%
CTCTTGTGTCCACACTAGGT+TGG - chr6_2:56031951-56031970 Msa0920680:CDS 50.0%
!! GTCCACACTAGGTTGGTTCA+AGG - chr6_2:56032064-56032083 Msa0920680:intron 50.0%
!! CCACACTAGGTTGGTTCAAG+GGG - chr6_2:56032066-56032085 Msa0920680:intron 50.0%
GCTAACCCCGTTTCTCCTAA+TGG + chr6_2:56032095-56032114 None:intergenic 50.0%
CGCAATCCCTATCTATCCGA+TGG + chr6_2:56032175-56032194 None:intergenic 50.0%
GGATAGATAGGGATTGCGCT+CGG - chr6_2:56032177-56032196 Msa0920680:intron 50.0%
GATAGATAGGGATTGCGCTC+GGG - chr6_2:56032178-56032197 Msa0920680:intron 50.0%
ATAGATAGGGATTGCGCTCG+GGG - chr6_2:56032179-56032198 Msa0920680:intron 50.0%
! ATTTTACGGAAAGCCCCGAG+TGG + chr6_2:56032210-56032229 None:intergenic 50.0%
ATACGTGTCATGGCGGTCAA+GGG + chr6_2:56032592-56032611 None:intergenic 50.0%
TATACGTGTCATGGCGGTCA+AGG + chr6_2:56032593-56032612 None:intergenic 50.0%
TTGACCGCCATGACACGTAT+AGG - chr6_2:56032592-56032611 Msa0920680:CDS 50.0%
CTTAGACGGTCGTGCATACT+AGG + chr6_2:56032657-56032676 None:intergenic 50.0%
TTTAGACACGTGGTGACCGT+AGG - chr6_2:56032720-56032739 Msa0920680:CDS 50.0%
TAGGCTATAGGCAACCTCAG+GGG + chr6_2:56032749-56032768 None:intergenic 50.0%
CTAGGCTATAGGCAACCTCA+GGG + chr6_2:56032750-56032769 None:intergenic 50.0%
ATATCCACATCGTCCTGCAG+CGG + chr6_2:56032891-56032910 None:intergenic 50.0%
!! CGTCTGAGGCAAGGGTATTC+TGG - chr6_2:56030950-56030969 Msa0920680:intron 55.0%
CTGCGCTAATAGCTCAGCTG+GGG + chr6_2:56031383-56031402 None:intergenic 55.0%
GCTGCGCTAATAGCTCAGCT+GGG + chr6_2:56031384-56031403 None:intergenic 55.0%
AGCTGCGCTAATAGCTCAGC+TGG + chr6_2:56031385-56031404 None:intergenic 55.0%
GAGCTATTAGCGCAGCTCTC+TGG - chr6_2:56031388-56031407 Msa0920680:intron 55.0%
TTAGCGCAGCTCTCTGGACA+TGG - chr6_2:56031394-56031413 Msa0920680:intron 55.0%
! GCCAACTTGCTTTGGCCTGT+TGG - chr6_2:56031416-56031435 Msa0920680:intron 55.0%
GCCAACAGGCCAAAGCAAGT+TGG + chr6_2:56031420-56031439 None:intergenic 55.0%
TGCACCACGGACTCTGAACT+GGG + chr6_2:56031442-56031461 None:intergenic 55.0%
ATGCACCACGGACTCTGAAC+TGG + chr6_2:56031443-56031462 None:intergenic 55.0%
TCGGATCGTATAGGCATCCG+AGG - chr6_2:56031852-56031871 Msa0920680:CDS 55.0%
! GTCCACACTAGGTTGGCTCA+AGG - chr6_2:56031958-56031977 Msa0920680:CDS 55.0%
! CCACACTAGGTTGGCTCAAG+GGG - chr6_2:56031960-56031979 Msa0920680:CDS 55.0%
!! GTGAAACGGGGTTAGCCGTA+AGG - chr6_2:56031993-56032012 Msa0920680:CDS 55.0%
CCCCTTGAACCAACCTAGTG+TGG + chr6_2:56032069-56032088 None:intergenic 55.0%
!! GAGAAACGGGGTTAGCCGTA+AGG - chr6_2:56032099-56032118 Msa0920680:intron 55.0%
AGTGACGTGCGGGATCCTTA+CGG + chr6_2:56032117-56032136 None:intergenic 55.0%
TACGTGTCATGGCGGTCAAG+GGG + chr6_2:56032591-56032610 None:intergenic 55.0%
GCAACCTCAGGGGAAACCTA+CGG + chr6_2:56032739-56032758 None:intergenic 55.0%
CCTAGGCTATAGGCAACCTC+AGG + chr6_2:56032751-56032770 None:intergenic 55.0%
CCTGAGGTTGCCTATAGCCT+AGG - chr6_2:56032748-56032767 Msa0920680:CDS 55.0%
ATCGGGTGGCCATGGACTCA+AGG + chr6_2:56029006-56029025 None:intergenic 60.0%
AGGACTCAATCGGGTGGCCA+TGG + chr6_2:56029014-56029033 None:intergenic 60.0%
AATGGGGCGGCCTAGAGAGA+AGG + chr6_2:56031077-56031096 None:intergenic 60.0%
AGCGTGGTTCTTCGGGTCTG+AGG + chr6_2:56031313-56031332 None:intergenic 60.0%
CTAATAGCTCAGCTGGGGCG+TGG + chr6_2:56031378-56031397 None:intergenic 60.0%
GGACTCTGAACTGGGCCAAC+AGG + chr6_2:56031434-56031453 None:intergenic 60.0%
TTGGCCCAGTTCAGAGTCCG+TGG - chr6_2:56031435-56031454 Msa0920680:intron 60.0%
CACGTCTGGCCACACTCGTA+TGG + chr6_2:56031775-56031794 None:intergenic 60.0%
CCCCTTGAGCCAACCTAGTG+TGG + chr6_2:56031963-56031982 None:intergenic 60.0%
!! CGTCACTGCAGGAGTGCCAT+TGG - chr6_2:56032130-56032149 Msa0920680:intron 60.0%
GTGACCGTAGGTTTCCCCTG+AGG - chr6_2:56032732-56032751 Msa0920680:CDS 60.0%
GTGTTCGTTCGTACCGCTGC+AGG - chr6_2:56032875-56032894 Msa0920680:intron 60.0%
AGGATCCCGCACGTCACTGC+AGG - chr6_2:56032119-56032138 Msa0920680:intron 65.0%
GCACTCCTGCAGTGACGTGC+GGG + chr6_2:56032127-56032146 None:intergenic 65.0%
GGCACTCCTGCAGTGACGTG+CGG + chr6_2:56032128-56032147 None:intergenic 65.0%
GCGCTCGGGGTTACTCCACT+CGG - chr6_2:56032192-56032211 Msa0920680:intron 65.0%
CGCTCGGGGTTACTCCACTC+GGG - chr6_2:56032193-56032212 Msa0920680:intron 65.0%
GCTCGGGGTTACTCCACTCG+GGG - chr6_2:56032194-56032213 Msa0920680:intron 65.0%
CGTACCGCTGCAGGACGATG+TGG - chr6_2:56032884-56032903 Msa0920680:intron 65.0%
GGGGCGGCCTAGAGAGAAGG+AGG + chr6_2:56031074-56031093 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 56028126 56033619 56028126 ID=Msa0920680;Name=Msa0920680
chr6_2 mRNA 56028126 56033619 56028126 ID=Msa0920680-mRNA-1;Parent=Msa0920680;Name=Msa0920680-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|1|0.14|0.25|8|0|396
chr6_2 exon 56033502 56033619 56033502 ID=Msa0920680-mRNA-1:exon:8395;Parent=Msa0920680-mRNA-1
chr6_2 exon 56032786 56032893 56032786 ID=Msa0920680-mRNA-1:exon:8394;Parent=Msa0920680-mRNA-1
chr6_2 exon 56032521 56032767 56032521 ID=Msa0920680-mRNA-1:exon:8393;Parent=Msa0920680-mRNA-1
chr6_2 exon 56032336 56032417 56032336 ID=Msa0920680-mRNA-1:exon:8392;Parent=Msa0920680-mRNA-1
chr6_2 exon 56032211 56032323 56032211 ID=Msa0920680-mRNA-1:exon:8391;Parent=Msa0920680-mRNA-1
chr6_2 exon 56032112 56032118 56032112 ID=Msa0920680-mRNA-1:exon:8390;Parent=Msa0920680-mRNA-1
chr6_2 exon 56031803 56032024 56031803 ID=Msa0920680-mRNA-1:exon:8389;Parent=Msa0920680-mRNA-1
chr6_2 exon 56028126 56028419 56028126 ID=Msa0920680-mRNA-1:exon:8388;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56033502 56033619 56033502 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56032786 56032893 56032786 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56032521 56032767 56032521 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56032336 56032417 56032336 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56032211 56032323 56032211 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56032112 56032118 56032112 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56031803 56032024 56031803 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
chr6_2 CDS 56028126 56028419 56028126 ID=Msa0920680-mRNA-1:cds;Parent=Msa0920680-mRNA-1
Gene Sequence

>Msa0920680

ATGAAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAACCTTCATTCTCTTGCTGTGCTATTTAAGGTGTTGAGGCAACATGGACTTTACGTTTCACCTGATGTGTTCAACCAATTCAAGGACGAGCAAGGAAACTTCAGTGAAACATTAGTCAGAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCACATATGATGGTTCATGAGGCGTTGGCTTTTACTTCTGGTCACCTTGAGTCCATGGCCACCCGATTGAGTCCTTCTCTTGCAACACAAGTAAAACAAAGCTTAAGACAATCTCTGCGCAAAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATCTATGAGCAAGATTCATCCCATAATGAAATTCTACTTGCGTTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAGGAATTTGGAAACATTGGCAAGTGGTGGAAGGAGTTGGATATTGCTCAAAAACTACCGTATGCACGAGATAGAGTAGTAGAATGTTGCTTTTGGGTTATGACCGGAAACTACCGTATGATGTCAAAATTTATCTCCATTTTATCATACATTGATGACACATATGATGCATATGGAACTATTGATGAATTAGAACTTTTTACTCAGGCAATTGAAAGTCATCAACCATTTTCTTCGCGTATTTCTACACGATATGATTCTTTTAACAATAGAGCAATGGTTAATATTGCAATCAAAATGATTTTTAGGTGGGATATTAATTGCTTGGAGGATCTTCCGGATTACACGAAGTTGCTTTATAAATCACTCTTGGATGTTTATCAAGAGATAGAGCAAGAAATGAAGAAAGAAGGAAGAGAATACACCCTTGACCCCTTCTTAAAAGAATTTGAGCAGAAAAGAGGACATGTTTCTTCGTACTTGGAATGTTATATGAGGGAATATGATTTGAATAGGGAAGCTGCCATTCAAGAATGCCAAAAGAAAATTGACAAAGCTTGGAAAGATATAAATCAGGAATGTCTTAAGCCAACTGAAGTTCCGTTACCTATTCTTACGTGTGTTCTCAATCTCTCGCGTTTTATTGAGGTTTTTTACAAAGATACAGATAACTACACACACTCTGACGGACTAATGAAGACATATATTAATGAATTGCTTGTCAATCCCGTAGAAATTTGA
Protein sequence

>Msa0920680

MKHIYKSYVQNGEITLKDNLHSLAVLFKVLRQHGLYVSPDVFNQFKDEQGNFSETLVRDVEGMLNLYEASHMMVHEALAFTSGHLESMATRLSPSLATQVKQSLRQSLRKNLPRLVARSYISIYEQDSSHNEILLALAKLDFNMLQSLHQKEFGNIGKWWKELDIAQKLPYARDRVVECCFWVMTGNYRMMSKFISILSYIDDTYDAYGTIDELELFTQAIESHQPFSSRISTRYDSFNNRAMVNIAIKMIFRWDINCLEDLPDYTKLLYKSLLDVYQEIEQEMKKEGREYTLDPFLKEFEQKRGHVSSYLECYMREYDLNREAAIQECQKKIDKAWKDINQECLKPTEVPLPILTCVLNLSRFIEVFYKDTDNYTHSDGLMKTYINELLVNPVEI*