AlfalfaGEDB | Msa0924550

M. sativa cultivar zhongmu-4 / Msa0924550

KEGG: K04730
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0924550	XP_013452957.1	38.806	134	77	1	439	567	192	325	4.26e-14	88.2
Msa0924550	XP_013452957.1	41.860	301	169	3	26	325	328	623	1.00e-65	236
Msa0924550	XP_013452957.1	84.211	456	43	4	1	452	1	431	0.0	777
Msa0924550	XP_013452957.1	90.435	115	11	0	454	568	510	624	5.71e-58	215

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0924550	sp\|Q9C6K9\|LRL11_ARATH	42.466	292	166	2	28	317	278	569	1.13e-62	220
Msa0924550	sp\|Q9C6K9\|LRL11_ARATH	61.111	72	28	0	379	450	308	379	1.50e-21	102
Msa0924550	sp\|Q9C6K9\|LRL11_ARATH	48.571	105	53	1	454	557	463	567	5.30e-19	94.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0266380	Msa0924550	0.809457	1.874500e-50	-8.615850e-47
Msa0341860	Msa0924550	0.805518	1.284105e-49	-8.615850e-47
Msa0521100	Msa0924550	0.802973	4.350872e-49	-8.615850e-47
Msa0897910	Msa0924550	0.831997	1.215582e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0924550	MtrunA17_Chr6g0483781	95.319	235	11	0	92	326	1	235	9.94e-164	465
Msa0924550	MtrunA17_Chr6g0483781	38.806	134	77	1	439	567	101	234	1.14e-19	88.6
Msa0924550	MtrunA17_Chr6g0483841	89.362	235	25	0	92	326	1	235	1.98e-152	438
Msa0924550	MtrunA17_Chr6g0483841	40.625	128	71	1	445	567	107	234	1.15e-19	89.4
Msa0924550	MtrunA17_Chr1g0209431	43.689	309	171	3	28	334	325	632	3.90e-77	257
Msa0924550	MtrunA17_Chr1g0209431	61.111	72	28	0	379	450	355	426	1.22e-22	102
Msa0924550	MtrunA17_Chr1g0209431	46.087	115	61	1	454	567	509	623	3.85e-19	91.3
Msa0924550	MtrunA17_Chr1g0209361	43.925	321	175	4	28	344	330	649	1.94e-75	253
Msa0924550	MtrunA17_Chr1g0209361	63.889	72	26	0	379	450	360	431	3.52e-23	103
Msa0924550	MtrunA17_Chr1g0209361	50.450	111	54	1	454	563	514	624	2.00e-22	101

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0924550	AT1G25390.1	42.466	292	166	2	28	317	278	569	1.15e-63	220
Msa0924550	AT1G25390.1	61.111	72	28	0	379	450	308	379	1.52e-22	102
Msa0924550	AT1G25390.1	48.571	105	53	1	454	557	463	567	5.39e-20	94.4
Msa0924550	AT1G25390.2	42.466	292	166	2	28	317	278	569	1.15e-63	220
Msa0924550	AT1G25390.2	61.111	72	28	0	379	450	308	379	1.52e-22	102
Msa0924550	AT1G25390.2	48.571	105	53	1	454	557	463	567	5.39e-20	94.4
Msa0924550	AT1G18390.2	38.871	319	190	4	28	344	331	646	1.38e-63	220
Msa0924550	AT1G18390.2	58.333	72	30	0	379	450	361	432	1.00e-20	96.7
Msa0924550	AT1G18390.2	45.045	111	60	1	454	563	515	625	2.85e-18	89.0
Msa0924550	AT1G18390.1	38.871	319	190	4	28	344	325	640	3.22e-63	219

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Find 105 sgRNAs with CRISPR-Local

Find 538 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTAAAGACGGTAGAG+AGG	0.641732	6_2:-66789428	Msa0924550:CDS
AAAAGCGACGTCTATAGCTT+TGG	0.439247	6_2:-66788947	Msa0924550:CDS
AAACCGGCAAGTATAATGCA+AGG	0.654254	6_2:-66788896	Msa0924550:CDS
AACAAGTGGTTATTTAGACC+CGG	0.594364	6_2:-66794197	Msa0924550:CDS
AACGAGTAAAATCTTAAACA+AGG	0.666290	6_2:-66788843	Msa0924550:CDS
AAGAAACTCTGGAAATTCTC+AGG	0.490412	6_2:+66796801	None:intergenic
AATTGATATAGCAAATGCAT+TGG	0.557792	6_2:-66794368	Msa0924550:CDS
ACAAGAATTAAGGCCTAACA+TGG	0.574191	6_2:-66793912	Msa0924550:CDS
ACAAGTACAACATATTGAAA+CGG	0.546848	6_2:-66794585	Msa0924550:CDS
ACCCCAGAAAAGTGTCAAAC+TGG	0.451802	6_2:+66793527	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTAGTTGGTTGCATAATT+TGG	-	chr6_2:66788742-66788761	Msa0924550:CDS	30.0%
TACAGTTGTTCTTGAATTTG+AGG	-	chr6_2:66788814-66788833	Msa0924550:CDS	30.0%
AAGAGTTTCATGTTTGTGTT+TGG	+	chr6_2:66788844-66788863	None:intergenic	30.0%
AGAAACTAATGAACAAGTTC+TGG	+	chr6_2:66789234-66789253	None:intergenic	30.0%
TCAAACTATTCGTTCTTAAC+CGG	+	chr6_2:66789365-66789384	None:intergenic	30.0%
ATTCAACCAGAATTTGAACT+CGG	+	chr6_2:66789391-66789410	None:intergenic	30.0%
TGGTAATTCAAAGTTCGATT+AGG	+	chr6_2:66789441-66789460	None:intergenic	30.0%
TTCTCAACTTTGTATCATAG+AGG	-	chr6_2:66789566-66789585	Msa0924550:intron	30.0%
CACATCAATTTATTCTGCTT+TGG	+	chr6_2:66789675-66789694	None:intergenic	30.0%
CAAAGCAGAATAAATTGATG+TGG	-	chr6_2:66789673-66789692	Msa0924550:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_2	gene	66788649	66796845	66788649	ID=Msa0924550;Name=Msa0924550
chr6_2	mRNA	66788649	66796845	66788649	ID=Msa0924550-mRNA-1;Parent=Msa0924550;Name=Msa0924550-mRNA-1;_AED=0.26;_eAED=0.26;_QI=87\|0.6\|0.5\|1\|0.8\|0.66\|6\|0\|568
chr6_2	exon	66788649	66788998	66788649	ID=Msa0924550-mRNA-1:exon:10582;Parent=Msa0924550-mRNA-1
chr6_2	exon	66789230	66789451	66789230	ID=Msa0924550-mRNA-1:exon:10581;Parent=Msa0924550-mRNA-1
chr6_2	exon	66793484	66793639	66793484	ID=Msa0924550-mRNA-1:exon:10580;Parent=Msa0924550-mRNA-1
chr6_2	exon	66793849	66794655	66793849	ID=Msa0924550-mRNA-1:exon:10579;Parent=Msa0924550-mRNA-1
chr6_2	exon	66795705	66795908	66795705	ID=Msa0924550-mRNA-1:exon:10578;Parent=Msa0924550-mRNA-1
chr6_2	exon	66796791	66796845	66796791	ID=Msa0924550-mRNA-1:exon:10577;Parent=Msa0924550-mRNA-1
chr6_2	five_prime_UTR	66796791	66796845	66796791	ID=Msa0924550-mRNA-1:five_prime_utr;Parent=Msa0924550-mRNA-1
chr6_2	five_prime_UTR	66795877	66795908	66795877	ID=Msa0924550-mRNA-1:five_prime_utr;Parent=Msa0924550-mRNA-1
chr6_2	CDS	66795705	66795876	66795705	ID=Msa0924550-mRNA-1:cds;Parent=Msa0924550-mRNA-1
chr6_2	CDS	66793849	66794655	66793849	ID=Msa0924550-mRNA-1:cds;Parent=Msa0924550-mRNA-1
chr6_2	CDS	66793484	66793639	66793484	ID=Msa0924550-mRNA-1:cds;Parent=Msa0924550-mRNA-1
chr6_2	CDS	66789230	66789451	66789230	ID=Msa0924550-mRNA-1:cds;Parent=Msa0924550-mRNA-1
chr6_2	CDS	66788649	66788998	66788649	ID=Msa0924550-mRNA-1:cds;Parent=Msa0924550-mRNA-1

Gene Sequence

>Msa0924550

ATGGGGTGGGATCTCTGTATGTGTTGCTGCTTCACTAGCACTTCTGACAAATTTCCAAAGCAGAATAAATTGATGTGGCAAATCTTCCCCCATGAGCAACTTAGAAAAGCCACAAATTATTTTGATCAAGCTCACATTCTTGAAAAGAAAGGCTCTGCCACTATATATTATGGAAAGCTTAAGAATGGCTGTGAGATTGCAGTCCAATGCTTCAATGAAGACAAGTACAACATATTGAAACGGTTCATCAATGAAGCTCAATTCCTAAATTACATGCCACACAAAAACCTAGTTACGATTTATGGTTATGCCACTCATCAAAAGGAATCCATGCTAGTACATGAATACCTGTCCAACGGGACTCTTGCTGCTCATATTCAAGGCCGAATATCAGATAGCGGAACATCATTAACTTGGCTTAACAGATTGGATATTGCAATTGATATAGCAAATGCATTGGAATATCTTCACTATAACGGCATTGTGCACCGTAATGTAAAGTCTCACAGTATTTTCCTTGATATAAATTTTTGTGCTAAAGTTGGTAACTTACATTTGTCAAAGAAACTTGATATTGGAGAAACCCGTGTTACAAGAGACTTAATCGGAACAAGTGGTTATTTAGACCCGGAACTTGTGTCAAAAGGCTTACTTGGTGTCCAATATGATGTTTATAGTTTTGGGGTGGTGTTGTGTGAGCTTGTGTCATCTATGCTTGCAGAATATTATATTCAGAATGAAGAAGAATGTCTGGCAACCCTTTTATGCAAAAAAGTTGAAAACCAAGCTTTAGTGGAGCTGTTGGATCCAAGACTTGGCTTCGAATCAGACCTGAAGATCAACAAGATGATGACTGCTACGGCTGAGCTTGCATTTTGGTGTTTGAAATGTCCACAAGAATTAAGGCCTAACATGGAACAAGTGCTAGAGTCTCTCCATGGCATAAAGCAAGGGAGATATGAGATAAATTCAATTAAAGAGATTGTTAATGTTTTAGACTCTTACTATGTAATTCATATGTCTCAGCTTTCAAAATTTTCCACCATGCCGAACTTGAAGAAGCTACTAACCAGTTTGACACTTTTCTGGGGTCCGGAGGGTTCGGCAGAGTCTACTATGGTAAGAAGCTGCAAAGGAAAACTTAAAGACGGTAGAGAGGTTGCAATAAAACGCTTCCACGAAGAGACAGAAAAAACCATTCAGCAGTTCATGAAAGAAATTGAGATCTTAAGTCACCTGCATCATCAAAATCTGGTTTCACTTTATGGTTGCAGTTCTCGTCATAGCAACAAGCACATGCTTGTGTACGAGTACATCTCTAATGGAACTCTTACACAACATCTTCACGGATCTTCATACTACTATGAAACTGGAAGGGTCAGTGAGAAAAGCGACGTCTATAGCTTTGGAGTGCTCTTATTTGAGCTTATATCATCGAAACCGGCAAGTATAATGCAAGGCACTGAGCATGTTACTCTTGCTCAATATGCAACGAGTAAAATCTTAAACAAGGAATTACAGCTGCTAGTGGATCAAAGTCTTCAAATTTCTTTTAATGAAAATGTAGTGGAAATGATAACAACAGTGACTGAGTTAGCATTTCAGTGTGTGCAGTGTCCCAAGGAACTCAGGCCAACCATGAAACAGGTTTTAGACACTCTACAGGGCATAAGCAAAGGGAAATGGGGATTCCACCAAATAACTTAG

Protein sequence

>Msa0924550

MGWDLCMCCCFTSTSDKFPKQNKLMWQIFPHEQLRKATNYFDQAHILEKKGSATIYYGKLKNGCEIAVQCFNEDKYNILKRFINEAQFLNYMPHKNLVTIYGYATHQKESMLVHEYLSNGTLAAHIQGRISDSGTSLTWLNRLDIAIDIANALEYLHYNGIVHRNVKSHSIFLDINFCAKVGNLHLSKKLDIGETRVTRDLIGTSGYLDPELVSKGLLGVQYDVYSFGVVLCELVSSMLAEYYIQNEEECLATLLCKKVENQALVELLDPRLGFESDLKINKMMTATAELAFWCLKCPQELRPNMEQVLESLHGIKQGRYEINSIKEIVNVLDSYYVIHMSQLSKFSTMPNLKKLLTSLTLFWGPEGSAESTMVRSCKGKLKDGREVAIKRFHEETEKTIQQFMKEIEILSHLHHQNLVSLYGCSSRHSNKHMLVYEYISNGTLTQHLHGSSYYYETGRVSEKSDVYSFGVLLFELISSKPASIMQGTEHVTLAQYATSKILNKELQLLVDQSLQISFNENVVEMITTVTELAFQCVQCPKELRPTMKQVLDTLQGISKGKWGFHQIT*