AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0266380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0266380 MtrunA17_Chr2g0331721 98.918 462 4 1 1 462 1 461 0.0 928
Msa0266380 MtrunA17_Chr7g0230011 80.087 462 73 2 1 462 1 443 0.0 723
Msa0266380 MtrunA17_Chr6g0458931 74.348 460 100 3 3 462 14 455 0.0 722
Msa0266380 MtrunA17_Chr6g0459021 74.943 435 108 1 28 462 14 447 0.0 657
Msa0266380 MtrunA17_Chr6g0459001 70.042 474 100 2 30 462 4 476 0.0 648
Msa0266380 MtrunA17_Chr8g0376991 67.586 435 140 1 28 462 3 436 0.0 639
Msa0266380 MtrunA17_Chr6g0458921 74.359 429 106 3 35 462 3 428 0.0 628
Msa0266380 MtrunA17_Chr3g0122921 66.895 438 126 2 25 462 10 428 0.0 619
Msa0266380 MtrunA17_Chr6g0459031 72.430 428 116 2 36 462 2 428 0.0 619
Msa0266380 MtrunA17_Chr4g0057851 67.554 413 133 1 48 460 10 421 0.0 606
Msa0266380 MtrunA17_Chr2g0328081 73.598 428 111 2 36 462 2 428 0.0 599
Msa0266380 MtrunA17_Chr6g0459011 76.142 394 93 1 64 457 1 393 0.0 585
Msa0266380 MtrunA17_Chr5g0422741 60.948 443 168 2 20 462 2 439 0.0 547
Msa0266380 MtrunA17_Chr1g0198531 59.862 436 169 3 27 462 8 437 0.0 530
Msa0266380 MtrunA17_Chr1g0198541 58.891 433 173 2 30 462 10 437 0.0 520
Msa0266380 MtrunA17_Chr3g0077651 52.874 435 202 2 31 462 35 469 4.89e-163 468
Msa0266380 MtrunA17_Chr3g0143701 36.797 462 270 9 4 448 39 495 7.53e-97 300
Msa0266380 MtrunA17_Chr5g0406331 37.061 456 263 8 3 439 37 487 2.16e-96 299
Msa0266380 MtrunA17_Chr2g0281341 37.269 432 260 5 40 461 87 517 8.98e-96 298
Msa0266380 MtrunA17_Chr4g0038711 35.332 467 272 7 5 449 29 487 5.60e-92 288
Msa0266380 MtrunA17_Chr5g0406351 35.794 447 274 6 28 462 87 532 6.72e-92 288
Msa0266380 MtrunA17_Chr3g0140761 30.595 487 286 13 12 458 1 475 9.46e-60 203
Msa0266380 MtrunA17_Chr1g0146491 30.687 466 269 12 35 458 12 465 2.86e-55 191
Msa0266380 MtrunA17_Chr1g0198081 28.782 476 310 12 1 462 19 479 1.66e-54 189
Msa0266380 MtrunA17_Chr4g0027241 28.211 475 311 11 1 461 1 459 5.93e-54 187
Msa0266380 MtrunA17_Chr8g0387941 28.194 454 302 10 24 461 2 447 9.50e-54 186
Msa0266380 MtrunA17_Chr3g0125091 28.542 480 285 12 28 458 3 473 1.29e-51 181
Msa0266380 MtrunA17_Chr3g0125101 29.424 469 281 12 26 458 19 473 4.83e-51 180
Msa0266380 MtrunA17_Chr3g0125081 29.095 464 283 12 31 458 19 472 6.45e-50 177
Msa0266380 MtrunA17_Chr1g0206871 28.446 457 272 10 49 458 16 464 8.08e-50 176
Msa0266380 MtrunA17_Chr3g0103271 28.721 477 285 13 23 457 2 465 2.20e-48 172
Msa0266380 MtrunA17_Chr4g0037201 26.994 489 302 11 10 458 32 505 4.43e-48 172
Msa0266380 MtrunA17_Chr3g0130641 30.435 460 262 12 40 458 28 470 6.29e-48 171
Msa0266380 MtrunA17_Chr1g0159761 30.134 448 261 13 25 433 5 439 4.77e-46 166
Msa0266380 MtrunA17_Chr5g0447221 26.884 491 290 12 1 452 1 461 7.84e-45 162
Msa0266380 MtrunA17_Chr3g0130651 28.571 441 267 11 21 428 6 431 8.08e-45 162
Msa0266380 MtrunA17_Chr1g0146411 28.702 439 266 14 26 427 7 435 1.52e-43 159
Msa0266380 MtrunA17_Chr1g0146391 27.623 467 285 14 35 462 2 454 2.96e-43 158
Msa0266380 MtrunA17_Chr8g0384661 27.640 445 279 10 49 458 51 487 4.54e-43 158
Msa0266380 MtrunA17_Chr1g0184721 26.049 453 294 13 30 461 5 437 3.26e-42 155
Msa0266380 MtrunA17_Chr3g0103231 27.212 452 291 10 33 452 10 455 6.52e-42 154
Msa0266380 MtrunA17_Chr3g0140541 26.697 442 274 11 47 449 24 454 1.27e-41 154
Msa0266380 MtrunA17_Chr5g0447231 27.765 443 271 14 49 452 26 458 3.02e-39 147
Msa0266380 MtrunA17_Chr3g0103211 27.434 452 290 10 33 452 10 455 5.32e-39 146
Msa0266380 MtrunA17_Chr3g0140531 25.996 477 301 16 26 461 2 467 7.91e-37 140
Msa0266380 MtrunA17_Chr1g0146331 25.330 454 279 13 35 443 10 448 2.79e-36 139
Msa0266380 MtrunA17_Chr2g0293331 25.566 442 267 11 49 443 13 439 3.21e-36 139
Msa0266380 MtrunA17_Chr3g0103221 24.766 428 288 9 56 452 38 462 3.52e-35 136
Msa0266380 MtrunA17_Chr4g0058161 24.449 454 324 12 14 461 7 447 4.64e-35 135
Msa0266380 MtrunA17_Chr1g0206851 27.920 351 207 10 143 458 35 374 1.10e-34 133
Msa0266380 MtrunA17_Chr1g0206831 29.355 310 180 8 177 457 37 336 2.38e-33 129
Msa0266380 MtrunA17_Chr5g0436281 29.049 389 258 12 76 458 1 377 6.06e-33 128
Msa0266380 MtrunA17_Chr3g0103251 25.610 328 190 7 177 452 43 368 3.76e-25 106
Msa0266380 MtrunA17_Chr1g0206821 28.899 218 126 6 264 458 313 524 2.09e-23 103
Msa0266380 MtrunA17_Chr1g0206821 32.365 241 134 7 49 267 23 256 2.89e-23 102
Msa0266380 MtrunA17_Chr3g0112971 25.789 380 252 13 94 461 72 433 5.61e-21 95.1
Msa0266380 MtrunA17_Chr5g0402151 29.496 278 162 7 59 329 11 261 9.07e-20 89.0
Msa0266380 MtrunA17_Chr3g0105861 23.684 456 309 15 12 454 1 430 7.97e-17 82.4
Msa0266380 MtrunA17_Chr3g0130661 28.736 174 108 2 301 458 1 174 1.62e-16 77.4
Msa0266380 MtrunA17_Chr7g0217401 24.876 402 252 16 32 417 25 392 4.66e-15 77.0
Msa0266380 MtrunA17_Chr7g0239011 21.127 426 293 15 19 431 32 427 7.79e-15 76.3
Msa0266380 MtrunA17_Chr8g0361631 21.814 408 279 14 26 425 2 377 2.22e-14 74.7
Msa0266380 MtrunA17_Chr7g0217381 24.874 398 249 15 38 416 24 390 7.54e-14 73.2
Msa0266380 MtrunA17_Chr4g0027261 26.702 191 124 4 280 461 1 184 3.10e-13 68.2
Msa0266380 MtrunA17_Chr3g0090101 24.934 377 241 15 58 421 2 349 7.45e-12 67.0
Msa0266380 MtrunA17_Chr3g0130671 28.846 208 125 7 49 241 40 239 1.07e-11 65.5
Msa0266380 MtrunA17_Chr6g0455951 22.199 482 294 19 1 443 3 442 4.67e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0266380 AT5G40780.1 77.753 445 92 2 24 462 3 446 0.0 733
Msa0266380 AT1G48640.1 72.789 441 116 2 25 462 14 453 0.0 672
Msa0266380 AT5G40780.3 79.198 399 82 1 64 462 1 398 0.0 670
Msa0266380 AT5G40780.2 79.198 399 82 1 64 462 1 398 0.0 670
Msa0266380 AT1G24400.1 74.318 440 112 1 23 462 3 441 0.0 664
Msa0266380 AT1G67640.1 74.318 440 111 2 23 462 4 441 0.0 649
Msa0266380 AT1G61270.1 65.734 429 146 1 21 448 6 434 0.0 593
Msa0266380 AT3G01760.1 64.747 434 146 4 29 456 9 441 0.0 586
Msa0266380 AT1G25530.1 60.369 434 166 2 28 461 12 439 0.0 527
Msa0266380 AT1G71680.1 55.902 449 191 3 20 462 1 448 2.62e-168 481
Msa0266380 AT1G71680.2 56.593 364 151 3 20 377 1 363 1.85e-142 412
Msa0266380 AT1G47670.1 37.269 432 260 5 40 461 85 515 2.76e-94 295
Msa0266380 AT4G35180.1 31.778 450 284 8 5 442 19 457 3.09e-73 239
Msa0266380 AT1G10010.1 33.118 465 268 11 30 458 11 468 6.19e-61 206
Msa0266380 AT1G10010.2 33.118 465 268 11 30 458 12 469 7.33e-61 206
Msa0266380 AT4G35180.2 34.304 309 191 3 5 310 19 318 1.27e-60 201
Msa0266380 AT1G08230.2 29.801 453 296 11 24 461 3 448 6.94e-54 187
Msa0266380 AT1G58360.1 30.533 488 282 12 8 458 10 477 3.19e-53 186
Msa0266380 AT5G41800.1 27.570 428 287 8 50 462 28 447 2.22e-50 177
Msa0266380 AT1G77380.1 30.682 440 253 13 32 433 15 440 9.16e-49 174
Msa0266380 AT5G49630.1 29.957 464 278 13 16 438 2 459 5.00e-48 172
Msa0266380 AT5G09220.1 28.571 448 275 11 49 458 46 486 9.21e-48 171
Msa0266380 AT1G44100.1 27.651 481 291 13 23 458 5 473 1.02e-44 163
Msa0266380 AT5G63850.1 27.477 444 285 8 49 458 19 459 4.47e-44 161
Msa0266380 AT5G23810.1 28.774 424 247 11 47 426 24 436 8.37e-40 149
Msa0266380 AT1G08230.3 30.423 355 227 10 120 461 1 348 3.25e-36 137
Msa0266380 AT1G08230.4 31.118 331 208 10 144 461 6 329 1.42e-34 132
Msa0266380 AT1G08230.6 31.118 331 208 10 144 461 6 329 1.42e-34 132
Msa0266380 AT1G08230.5 31.118 331 208 10 144 461 6 329 1.42e-34 132
Msa0266380 AT3G55740.1 26.667 465 302 16 1 458 1 433 2.55e-32 128
Msa0266380 AT2G39890.2 25.000 448 311 14 23 458 2 436 2.93e-30 122
Msa0266380 AT2G39890.1 25.000 448 311 14 23 458 2 436 2.93e-30 122
Msa0266380 AT2G36590.1 26.316 418 283 15 28 434 3 406 4.74e-30 121
Msa0266380 AT3G55740.2 26.992 389 266 12 76 458 1 377 3.94e-29 118
Msa0266380 AT3G55740.3 26.992 389 266 12 76 458 1 377 3.94e-29 118
Msa0266380 AT2G39890.3 25.385 390 271 11 76 458 1 377 9.08e-29 117
Msa0266380 AT5G23810.2 28.528 326 197 9 47 347 24 338 2.44e-26 110
Msa0266380 AT2G36590.2 27.554 323 213 13 28 342 3 312 1.11e-20 93.2
Msa0266380 AT5G15240.1 25.183 409 242 16 31 416 16 383 2.55e-17 84.3
Msa0266380 AT5G23810.4 27.686 242 143 7 47 265 24 256 1.97e-15 76.6
Msa0266380 AT2G39130.2 23.679 473 304 20 6 452 104 545 2.58e-15 78.6
Msa0266380 AT2G39130.1 23.679 473 304 20 6 452 104 545 2.58e-15 78.6
Msa0266380 AT5G02180.1 22.739 387 266 11 43 420 151 513 6.30e-14 74.3
Msa0266380 AT5G02180.2 22.739 387 266 11 43 420 151 513 6.30e-14 74.3
Msa0266380 AT5G23810.5 25.333 225 137 7 25 230 5 217 4.37e-12 66.2
Msa0266380 AT5G23810.3 25.333 225 137 7 25 230 5 217 4.37e-12 66.2

Find 143 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTCCCTGTTGCTCTTAT+TGG 0.168322 2_2:+66870300 Msa0266380:CDS
TTCAATTTCTTCAGTGCTTT+AGG 0.228996 2_2:+66870141 Msa0266380:CDS
GCATTCCTTGAAGAAGTTAT+TGG 0.252163 2_2:-66867064 None:intergenic
GGAATAACCTTCCCATTCTT+TGG 0.269371 2_2:+66870897 Msa0266380:CDS
ATTTCCCTGTTGCTCTTATT+GGG 0.271137 2_2:+66870301 Msa0266380:CDS
CCATTCTTTGGTGCCCTCCT+TGG 0.281653 2_2:+66870909 Msa0266380:CDS
TTTGTTGTCATTCATGTTAT+AGG 0.284943 2_2:+66870408 Msa0266380:CDS
TTTCTATACTTGACGATTCA+TGG 0.294299 2_2:+66871331 Msa0266380:exon
GCTTGTCTTGGTTCACAAAC+TGG 0.295561 2_2:+66871121 Msa0266380:CDS
CTGTTGCTCTTATTGGGTAT+TGG 0.300006 2_2:+66870307 Msa0266380:CDS
CAAGACAAGCTAAACCTCTT+AGG 0.301943 2_2:-66871108 None:intergenic
ACTGCAGAAAATTCATGATT+TGG 0.311368 2_2:+66868850 Msa0266380:CDS
CCCAACTTCAACTCCATTGC+TGG 0.312413 2_2:+66868956 Msa0266380:CDS
CTTATTGGGTATTGGATGTT+TGG 0.317962 2_2:+66870315 Msa0266380:CDS
CTTCTTCAAGGAATGCAAAA+TGG 0.331015 2_2:+66867071 Msa0266380:CDS
ATGACAAAGGCAGGTCTAAA+AGG 0.331685 2_2:-66871376 None:intergenic
AAGAAATTGAAAACAGTTCC+TGG 0.370996 2_2:-66870129 None:intergenic
TAATCACCCTCTACACTCTA+TGG 0.375402 2_2:+66868660 Msa0266380:CDS
CATTGCGAACAACAAATCTT+AGG 0.383519 2_2:-66870611 None:intergenic
CAATACCCAATAAGAGCAAC+AGG 0.389191 2_2:-66870306 None:intergenic
TGCTTCTTCAGAGGCTGAAA+AGG 0.398966 2_2:+66866882 Msa0266380:CDS
TTGTAGCCATAGGCCACATC+TGG 0.403065 2_2:-66870093 None:intergenic
GAGATGCATGAAATGGTTCC+AGG 0.408422 2_2:+66868692 Msa0266380:CDS
TGTAGCTTCCTTGCATTATG+TGG 0.411632 2_2:+66871070 Msa0266380:intron
TTGGAGAAAAGCTTGGACTC+TGG 0.412306 2_2:+66868756 Msa0266380:CDS
AACAAAAGGCAATTGATGAT+TGG 0.412955 2_2:+66867038 Msa0266380:CDS
TCAGCCTCTGAAGAAGCAAT+TGG 0.414783 2_2:-66866877 None:intergenic
TCATGTTATAGGAAGCTATC+AGG 0.416323 2_2:+66870419 Msa0266380:CDS
TCCTCTCCACATTGGACCCT+TGG 0.420922 2_2:-66870244 None:intergenic
ATTAGCCTCAATCCACCTAT+TGG 0.422145 2_2:-66871284 None:intergenic
CTCCACATTGGACCCTTGGA+TGG 0.422755 2_2:-66870240 None:intergenic
GGCTTCCAATAACTTCTTCA+AGG 0.425061 2_2:+66867059 Msa0266380:CDS
TCATGGATGCATGTGTATAA+TGG 0.429106 2_2:+66871348 Msa0266380:three_prime_UTR
TTCAAATTGTTTGCAATGGA+GGG 0.431140 2_2:+66871475 Msa0266380:three_prime_UTR
CCATCTGCCATGGCAAGTCT+TGG 0.434758 2_2:+66867151 Msa0266380:CDS
CCAAGGAGGGCACCAAAGAA+TGG 0.436204 2_2:-66870909 None:intergenic
CTAAGAGGTTTAGCTTGTCT+TGG 0.437451 2_2:+66871109 Msa0266380:CDS
GGACCCTTGGATGGTTTCTC+CGG 0.438031 2_2:-66870231 None:intergenic
CTTATGATTCTGTCACCAAT+AGG 0.442214 2_2:+66871269 Msa0266380:CDS
CTTCTCTTAATAAACCTACT+TGG 0.442717 2_2:+66870364 Msa0266380:CDS
GACATTGTTTACATGGTCAC+CGG 0.442865 2_2:+66868818 Msa0266380:CDS
CTCCATTGCTGGTATCTCCT+TGG 0.443891 2_2:+66868967 Msa0266380:CDS
TTTCAGATTTGCATTATACT+TGG 0.449641 2_2:+66871239 Msa0266380:intron
GATGGTTTCTCCGGTGTGGA+CGG 0.451778 2_2:-66870222 None:intergenic
TTCAGCATTCACAATGTTTG+TGG 0.454604 2_2:+66870875 Msa0266380:intron
ATGGTTTCTCCGGTGTGGAC+GGG 0.455777 2_2:-66870221 None:intergenic
GGTGCTTTGATTGGAACAAA+TGG 0.456812 2_2:+66871434 Msa0266380:three_prime_UTR
TAAAAGAACGGCTGAACAAA+AGG 0.459944 2_2:+66867024 Msa0266380:CDS
ACCAGCAATGGAGTTGAAGT+TGG 0.460631 2_2:-66868957 None:intergenic
CTCCAACACCACATTGTGTC+CGG 0.463600 2_2:-66870184 None:intergenic
GATTGGAACAAATGGAGATA+AGG 0.464708 2_2:+66871442 Msa0266380:three_prime_UTR
CTGCAGCAATCATGTCTTTG+AGG 0.471568 2_2:+66868990 Msa0266380:CDS
ATGAAATGGTTCCAGGAAAA+CGG 0.476078 2_2:+66868699 Msa0266380:CDS
TGGAGTTGAAGTTGGGAAGA+TGG 0.477812 2_2:-66868949 None:intergenic
ATGTCGAATTGCAGGGGTCC+TGG 0.479412 2_2:+66868611 Msa0266380:intron
AGTATAGAAACCGTAGGACT+TGG 0.487333 2_2:-66871318 None:intergenic
CTTGTCTTGGTTCACAAACT+GGG 0.488155 2_2:+66871122 Msa0266380:CDS
GGAGTTGAAGTTGGGAAGAT+GGG 0.488377 2_2:-66868948 None:intergenic
TCAGCATTCACAATGTTTGT+GGG 0.488938 2_2:+66870876 Msa0266380:intron
GGACTTACAAAATATGTTGT+GGG 0.491845 2_2:-66870957 None:intergenic
ATTCAAATTGTTTGCAATGG+AGG 0.493032 2_2:+66871474 Msa0266380:three_prime_UTR
CCAAGACTTGCCATGGCAGA+TGG 0.495959 2_2:-66867151 None:intergenic
CAAGGAGGGCACCAAAGAAT+GGG 0.496713 2_2:-66870908 None:intergenic
CCTCTACACTCTATGGCAAA+TGG 0.497407 2_2:+66868667 Msa0266380:CDS
TTGCTATTTACAAACCTAAG+AGG 0.497836 2_2:+66871094 Msa0266380:CDS
AACAACAAATCTTAGGAGTC+GGG 0.497913 2_2:-66870604 None:intergenic
TATATTCAAATTGTTTGCAA+TGG 0.499086 2_2:+66871471 Msa0266380:three_prime_UTR
TTTGACAGATACCATGAGTT+GGG 0.500029 2_2:+66868722 Msa0266380:CDS
TGACATGATTGAGACCGTAA+TGG 0.504825 2_2:+66870557 Msa0266380:CDS
GAGTTGGGACAAGAAGCATT+TGG 0.508176 2_2:+66868737 Msa0266380:CDS
GAAGCATTTGGAGAAAAGCT+TGG 0.510904 2_2:+66868749 Msa0266380:CDS
TGTTGGAGACTCAGTTCCCA+TGG 0.511962 2_2:-66866845 None:intergenic
TGGACTTACAAAATATGTTG+TGG 0.515751 2_2:-66870958 None:intergenic
CACAACGTTACTGCCATGGT+TGG 0.517108 2_2:+66867109 Msa0266380:CDS
CCAGCAATGGAGTTGAAGTT+GGG 0.520549 2_2:-66868956 None:intergenic
ATGCAAGATATGACAAAGGC+AGG 0.523499 2_2:-66871385 None:intergenic
GTTGTTCGCAATGTTTATGT+TGG 0.530022 2_2:+66870621 Msa0266380:CDS
GCTGCAACTATAAGCCAAGT+AGG 0.535757 2_2:-66870378 None:intergenic
TCAAATTGTTTGCAATGGAG+GGG 0.540207 2_2:+66871476 Msa0266380:three_prime_UTR
CTTGAGTCTTCCATCTGCCA+TGG 0.541643 2_2:+66867141 Msa0266380:CDS
ATTGTTTGCAATGGAGGGGA+GGG 0.545992 2_2:+66871480 Msa0266380:three_prime_UTR
ATGCTTCTTGTCCCAACTCA+TGG 0.547647 2_2:-66868733 None:intergenic
GTTCAACCAGATGTGGCCTA+TGG 0.548195 2_2:+66870087 Msa0266380:CDS
GAACAACAAATCTTAGGAGT+CGG 0.552204 2_2:-66870605 None:intergenic
AGTTGTGGCTTTGTAGCCAT+AGG 0.553663 2_2:-66870103 None:intergenic
TGGTGTGGACATTGTTTACA+TGG 0.554021 2_2:+66868811 Msa0266380:CDS
AATGGTTGAGATGCATGAAA+TGG 0.555074 2_2:+66868685 Msa0266380:CDS
AGACATGATTGCTGCAGCCA+AGG 0.555144 2_2:-66868984 None:intergenic
CTGCCATGGCAAGTCTTGGA+TGG 0.557557 2_2:+66867155 Msa0266380:CDS
ACATTGTTTACATGGTCACC+GGG 0.558322 2_2:+66868819 Msa0266380:CDS
GTAGTTACTCAACAATTGCA+TGG 0.558837 2_2:+66870043 Msa0266380:intron
GTTTGACAGATACCATGAGT+TGG 0.571800 2_2:+66868721 Msa0266380:CDS
AACAGTTCCTGGTGTAGTTG+TGG 0.571899 2_2:-66870118 None:intergenic
AATTGTTTGCAATGGAGGGG+AGG 0.572605 2_2:+66871479 Msa0266380:three_prime_UTR
AATAAAACTCCTCTCCACAT+TGG 0.575187 2_2:-66870252 None:intergenic
CAACAGTTGATATGTGAAGT+TGG 0.577281 2_2:+66868791 Msa0266380:CDS
ATACTCAATGCCAAGTCCTA+CGG 0.577308 2_2:+66871308 Msa0266380:CDS
TTACTCAACAATTGCATGGG+TGG 0.581664 2_2:+66870047 Msa0266380:CDS
ACAAATCTTAGGAGTCGGGT+TGG 0.583916 2_2:-66870600 None:intergenic
AAAACCAATTGCTTCTTCAG+AGG 0.589072 2_2:+66866873 Msa0266380:CDS
GTTACTGCCATGGTTGGAGC+TGG 0.590710 2_2:+66867115 Msa0266380:CDS
CTTGGATGGTTTCTCCGGTG+TGG 0.592081 2_2:-66870226 None:intergenic
CATTTGCCATAGAGTGTAGA+GGG 0.594361 2_2:-66868666 None:intergenic
CTTGACTTAATAACCACCAT+GGG 0.594664 2_2:+66866829 Msa0266380:exon
CAAGACACCAGCTCCAACCA+TGG 0.596093 2_2:-66867122 None:intergenic
TAGTTACTCAACAATTGCAT+GGG 0.600051 2_2:+66870044 Msa0266380:intron
TGCCGGACACAATGTGGTGT+TGG 0.600214 2_2:+66870182 Msa0266380:CDS
ATGATTCTGTCACCAATAGG+TGG 0.600362 2_2:+66871272 Msa0266380:CDS
TACAAAGCCACAACTACACC+AGG 0.601821 2_2:+66870111 Msa0266380:CDS
TGTCACCAATAGGTGGATTG+AGG 0.602435 2_2:+66871279 Msa0266380:CDS
GATGTTGCATTTGCCTATGC+CGG 0.602926 2_2:+66870165 Msa0266380:CDS
GTGACAGAATCATAAGGAGG+AGG 0.610650 2_2:-66871262 None:intergenic
AAAGGGAGTTCAACCAGATG+TGG 0.615250 2_2:+66870080 Msa0266380:CDS
CCATTTGCCATAGAGTGTAG+AGG 0.615329 2_2:-66868667 None:intergenic
ATAGCAAGCCACATAATGCA+AGG 0.617411 2_2:-66871078 None:intergenic
AAAATTAGAATCACCACACC+AGG 0.621042 2_2:-66868629 None:intergenic
TTAACAGAGAGATAAAAGAA+CGG 0.624935 2_2:+66867012 Msa0266380:intron
ACTTGACTTAATAACCACCA+TGG 0.625403 2_2:+66866828 Msa0266380:exon
ACTTCACATATCAACTGTTG+TGG 0.626823 2_2:-66868788 None:intergenic
TGGAGACTCAGTTCCCATGG+TGG 0.630497 2_2:-66866842 None:intergenic
ATACCATCCAAGACTTGCCA+TGG 0.631568 2_2:-66867158 None:intergenic
GAATTGCAGGGGTCCTGGTG+TGG 0.635436 2_2:+66868616 Msa0266380:intron
ATTGTTTACATGGTCACCGG+GGG 0.638235 2_2:+66868821 Msa0266380:CDS
CTATTGGTGACAGAATCATA+AGG 0.645120 2_2:-66871268 None:intergenic
TGTTTGTGTCAACACTAGGA+CGG 0.646719 2_2:+66866691 None:intergenic
TCCAAGGGTCCAATGTGGAG+AGG 0.647648 2_2:+66870243 Msa0266380:CDS
CACCACATTGTGTCCGGCAT+AGG 0.647925 2_2:-66870178 None:intergenic
TAAAATGCAAGATATGACAA+AGG 0.648706 2_2:-66871389 None:intergenic
GGCAAGAATAAAATGAACAG+AGG 0.650976 2_2:-66868927 None:intergenic
AGCAACAATCCCGTCCACAC+CGG 0.654369 2_2:+66870212 Msa0266380:CDS
CTTCAAGGAATGCAAAATGG+TGG 0.654515 2_2:+66867074 Msa0266380:CDS
GTTGATATGTGAAGTTGGTG+TGG 0.658649 2_2:+66868796 Msa0266380:CDS
CACACCGGAGAAACCATCCA+AGG 0.663681 2_2:+66870227 Msa0266380:CDS
AGCCAAGGAGATACCAGCAA+TGG 0.663690 2_2:-66868969 None:intergenic
TTTCTCAGCACAAGAACCAG+AGG 0.673739 2_2:+66866909 Msa0266380:CDS
CATTGTTTACATGGTCACCG+GGG 0.675950 2_2:+66868820 Msa0266380:CDS
GAGGGCACCAAAGAATGGGA+AGG 0.676285 2_2:-66870904 None:intergenic
TCGTCAAGTATAGAAACCGT+AGG 0.681478 2_2:-66871324 None:intergenic
AACCATCCAAGGGTCCAATG+TGG 0.683222 2_2:+66870238 Msa0266380:CDS
ACACCGGAGAAACCATCCAA+GGG 0.697058 2_2:+66870228 Msa0266380:CDS
TTGGTGACAGAATCATAAGG+AGG 0.701093 2_2:-66871265 None:intergenic
TGCCTATGCCGGACACAATG+TGG 0.720388 2_2:+66870176 Msa0266380:CDS
AATACCCAATAAGAGCAACA+GGG 0.735246 2_2:-66870305 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTATATTTTTTAAA+TGG - chr2_2:66867659-66867678 None:intergenic 0.0%
!!! ATTTATATTTTTTAAATGGT+TGG - chr2_2:66867655-66867674 None:intergenic 10.0%
!! ATTTGAATATTAATGAAATA+TGG - chr2_2:66869635-66869654 None:intergenic 10.0%
!!! TATATAAAATAAAAGTACAA+CGG + chr2_2:66869842-66869861 Msa0266380:intron 10.0%
!!! TATGTGTAGTTTAAATTTTT+TGG - chr2_2:66866807-66866826 None:intergenic 15.0%
!! TGTCAAAAAAAAAAAAACAA+GGG - chr2_2:66867414-66867433 None:intergenic 15.0%
!! TTGTCAAAAAAAAAAAAACA+AGG - chr2_2:66867415-66867434 None:intergenic 15.0%
!!! AATTTAACTTCTAGAAAAAA+AGG + chr2_2:66867498-66867517 Msa0266380:intron 15.0%
!! AGAAAATATTTAAGAAAAGA+AGG - chr2_2:66869058-66869077 None:intergenic 15.0%
!! ATAATATTATGTTATACCAT+TGG - chr2_2:66869319-66869338 None:intergenic 15.0%
!!! GAAAATAGATTATTTTTTTG+TGG - chr2_2:66869966-66869985 None:intergenic 15.0%
!!! TATTTTTTAACTAAACTTGA+TGG + chr2_2:66869994-66870013 Msa0266380:intron 15.0%
!!! TAAAATATCCAATTTTAGAA+AGG - chr2_2:66870653-66870672 None:intergenic 15.0%
!! TAAAACATAACTTATCTTAT+TGG + chr2_2:66871206-66871225 Msa0266380:intron 15.0%
!!! TTTTCTTTACTTTTTTTGTA+AGG + chr2_2:66871412-66871431 Msa0266380:three_prime_UTR 15.0%
!!! TTTCTTTACTTTTTTTGTAA+GGG + chr2_2:66871413-66871432 Msa0266380:three_prime_UTR 15.0%
!!! TTTTGTTTTCAAGAAAAAAA+TGG + chr2_2:66871538-66871557 Msa0266380:three_prime_UTR 15.0%
!!! TGTCAATAAATGTTTTGATA+TGG - chr2_2:66866757-66866776 None:intergenic 20.0%
!!! TTAAATTTTTTGGAACAAAG+AGG - chr2_2:66866797-66866816 None:intergenic 20.0%
!!! AACTAGAATTTTTCAAACAA+GGG - chr2_2:66867306-66867325 None:intergenic 20.0%
!!! AAACTAGAATTTTTCAAACA+AGG - chr2_2:66867307-66867326 None:intergenic 20.0%
!! AGTTTAAACAATAACCTAAA+CGG - chr2_2:66867445-66867464 None:intergenic 20.0%
!! ACTATTCACTTATTAGAAAA+TGG + chr2_2:66867462-66867481 Msa0266380:intron 20.0%
!! AATAATAATAATACACCCTT+TGG - chr2_2:66867689-66867708 None:intergenic 20.0%
!!! AGTTTTGAAATTTGAGAATT+GGG - chr2_2:66867768-66867787 None:intergenic 20.0%
!!! TAGTTTTGAAATTTGAGAAT+TGG - chr2_2:66867769-66867788 None:intergenic 20.0%
!! TTCTCAAATTTCAAAACTAT+TGG + chr2_2:66867770-66867789 Msa0266380:intron 20.0%
!! CAATAAACACTTACTTAATT+AGG - chr2_2:66867833-66867852 None:intergenic 20.0%
!! CTAATTAAGTAAGTGTTTAT+TGG + chr2_2:66867831-66867850 Msa0266380:intron 20.0%
!! GTTCATTATGCATTATTTAT+AGG + chr2_2:66867868-66867887 Msa0266380:intron 20.0%
!!! ATCAAGTTTGATTTTAATCA+TGG - chr2_2:66867901-66867920 None:intergenic 20.0%
!! TTTAGTAGTATTTATCAACA+TGG - chr2_2:66869122-66869141 None:intergenic 20.0%
!!! TGTTGATAAATACTACTAAA+AGG + chr2_2:66869122-66869141 Msa0266380:intron 20.0%
!! TATTCAACTTTATTGAGAAT+GGG - chr2_2:66869551-66869570 None:intergenic 20.0%
!! ATATTCAACTTTATTGAGAA+TGG - chr2_2:66869552-66869571 None:intergenic 20.0%
!! TACATAATATATGTAAGATC+AGG + chr2_2:66869924-66869943 Msa0266380:intron 20.0%
!! ACATAATATATGTAAGATCA+GGG + chr2_2:66869925-66869944 Msa0266380:intron 20.0%
!! ATTTACTTTGATATCTTTAG+AGG + chr2_2:66871175-66871194 Msa0266380:intron 20.0%
!! TATATTCAAATTGTTTGCAA+TGG + chr2_2:66871471-66871490 Msa0266380:three_prime_UTR 20.0%
! TTAACAGAGAGATAAAAGAA+CGG + chr2_2:66867012-66867031 Msa0266380:intron 25.0%
! TATTAGAAAATGGATGTTCA+TGG + chr2_2:66867472-66867491 Msa0266380:intron 25.0%
! ATTAGAAAATGGATGTTCAT+GGG + chr2_2:66867473-66867492 Msa0266380:intron 25.0%
!!! TTTTAAATGGTTGGTATAGA+AGG - chr2_2:66867646-66867665 None:intergenic 25.0%
! TAAATAAAAAAAGTGGCCAA+AGG + chr2_2:66867670-66867689 Msa0266380:intron 25.0%
! AAATAAAAAAAGTGGCCAAA+GGG + chr2_2:66867671-66867690 Msa0266380:intron 25.0%
! TAAAGAAGAATAGAAACTTG+CGG - chr2_2:66867721-66867740 None:intergenic 25.0%
!! ATCAAACCATTTTCATAAGT+TGG + chr2_2:66867919-66867938 Msa0266380:intron 25.0%
!!! TATCGCGATACAATTTTTTT+AGG + chr2_2:66868052-66868071 Msa0266380:intron 25.0%
!!! ATTCCTAGATGAACATTTTT+TGG + chr2_2:66868427-66868446 Msa0266380:intron 25.0%
!!! ATTTTTTTGCGTTAACACTT+CGG - chr2_2:66868513-66868532 None:intergenic 25.0%
!! TGGTGATTCTAATTTTATCA+TGG + chr2_2:66868636-66868655 Msa0266380:CDS 25.0%
! ACTAAAAGGTAAATAGAGAT+TGG + chr2_2:66869136-66869155 Msa0266380:intron 25.0%
! TTATATAAGCTCATCAATAG+AGG + chr2_2:66869476-66869495 Msa0266380:intron 25.0%
! TTTAGGTGTCTAAATTGATT+CGG + chr2_2:66869724-66869743 Msa0266380:intron 25.0%
!!! CAATCATATTTTAGCTAAGT+TGG + chr2_2:66869892-66869911 Msa0266380:intron 25.0%
!!! ATATTTTAGCTAAGTTGGTA+GGG + chr2_2:66869897-66869916 Msa0266380:intron 25.0%
!! TTTGTTGTCATTCATGTTAT+AGG + chr2_2:66870408-66870427 Msa0266380:CDS 25.0%
!! AGCTAAACAAATAGCAATTT+TGG - chr2_2:66870832-66870851 None:intergenic 25.0%
! TTTCAGATTTGCATTATACT+TGG + chr2_2:66871239-66871258 Msa0266380:intron 25.0%
! TAAAATGCAAGATATGACAA+AGG - chr2_2:66871392-66871411 None:intergenic 25.0%
! AGAAAAAAATGGAGTGATTT+AGG + chr2_2:66871549-66871568 Msa0266380:three_prime_UTR 25.0%
!!! GCAATTGGTTTTTCATTTGT+TGG - chr2_2:66866865-66866884 None:intergenic 30.0%
TATGTTGTTAAGTTTACCTC+TGG - chr2_2:66866928-66866947 None:intergenic 30.0%
! AACAAAAGGCAATTGATGAT+TGG + chr2_2:66867038-66867057 Msa0266380:CDS 30.0%
!! GAACTTCTAGTTCTAGAATA+CGG - chr2_2:66867224-66867243 None:intergenic 30.0%
!! ATTCTAGAACTAGAAGTTCA+TGG + chr2_2:66867225-66867244 Msa0266380:intron 30.0%
CTCATATTCTCATACTTGAT+AGG - chr2_2:66867261-66867280 None:intergenic 30.0%
!! ATTTTTCAAACAAGGGTTGA+CGG - chr2_2:66867299-66867318 None:intergenic 30.0%
AATATGAGTGACCATATGAT+TGG + chr2_2:66867328-66867347 Msa0266380:intron 30.0%
TCAAACTTGTACCAATCATA+TGG - chr2_2:66867342-66867361 None:intergenic 30.0%
GGGATTTGACATGTAATTTA+CGG - chr2_2:66867748-66867767 None:intergenic 30.0%
TAGCTACCAACTTATGAAAA+TGG - chr2_2:66867928-66867947 None:intergenic 30.0%
TATTACCTCTCGAACAAATT+GGG + chr2_2:66868348-66868367 Msa0266380:intron 30.0%
TGACCAAAAAATGTTCATCT+AGG - chr2_2:66868433-66868452 None:intergenic 30.0%
! TTTTACAATGTCGAATTGCA+GGG + chr2_2:66868604-66868623 Msa0266380:intron 30.0%
ACTGCAGAAAATTCATGATT+TGG + chr2_2:66868850-66868869 Msa0266380:CDS 30.0%
AAACGACCAAATTGAAGAAT+TGG - chr2_2:66869182-66869201 None:intergenic 30.0%
TCTTCAATTTGGTCGTTTAA+TGG + chr2_2:66869184-66869203 Msa0266380:intron 30.0%
!! CAATTTTGAAGATGATGTGT+TGG + chr2_2:66869264-66869283 Msa0266380:intron 30.0%
!! AAGTCTTTCTAAAGTCTCTA+TGG - chr2_2:66869395-66869414 None:intergenic 30.0%
! ATAGAGACTTTAGAAAGACT+TGG + chr2_2:66869394-66869413 Msa0266380:intron 30.0%
! GATTGGATTGTTCTTTTAGT+CGG - chr2_2:66869520-66869539 None:intergenic 30.0%
TCAAATTGGTCACAATATGT+GGG - chr2_2:66869701-66869720 None:intergenic 30.0%
! ATTTCTGGACTAAAGTCAAA+TGG - chr2_2:66869819-66869838 None:intergenic 30.0%
!!! CATATTTTAGCTAAGTTGGT+AGG + chr2_2:66869896-66869915 Msa0266380:intron 30.0%
TAGTTACTCAACAATTGCAT+GGG + chr2_2:66870044-66870063 Msa0266380:intron 30.0%
AAGAAATTGAAAACAGTTCC+TGG - chr2_2:66870132-66870151 None:intergenic 30.0%
TTCAATTTCTTCAGTGCTTT+AGG + chr2_2:66870141-66870160 Msa0266380:CDS 30.0%
CTTCTCTTAATAAACCTACT+TGG + chr2_2:66870364-66870383 Msa0266380:CDS 30.0%
TGTGAAGAATCATATGCAAA+TGG - chr2_2:66870468-66870487 None:intergenic 30.0%
!!! ACGCAATTTTTTGACCATTA+CGG - chr2_2:66870574-66870593 None:intergenic 30.0%
! ATTTTATCGTTATCCGATGA+TGG + chr2_2:66870761-66870780 Msa0266380:intron 30.0%
GGACTTACAAAATATGTTGT+GGG - chr2_2:66870960-66870979 None:intergenic 30.0%
TGGACTTACAAAATATGTTG+TGG - chr2_2:66870961-66870980 None:intergenic 30.0%
!! TTGCTATTTACAAACCTAAG+AGG + chr2_2:66871094-66871113 Msa0266380:CDS 30.0%
TTTCTATACTTGACGATTCA+TGG + chr2_2:66871331-66871350 Msa0266380:exon 30.0%
ATTCAAATTGTTTGCAATGG+AGG + chr2_2:66871474-66871493 Msa0266380:three_prime_UTR 30.0%
TTCAAATTGTTTGCAATGGA+GGG + chr2_2:66871475-66871494 Msa0266380:three_prime_UTR 30.0%
!!! ATTTTAGCTCTCTCAGAAAA+TGG + chr2_2:66871510-66871529 Msa0266380:three_prime_UTR 30.0%
ACTTGACTTAATAACCACCA+TGG + chr2_2:66866828-66866847 Msa0266380:exon 35.0%
CTTGACTTAATAACCACCAT+GGG + chr2_2:66866829-66866848 Msa0266380:exon 35.0%
AAAACCAATTGCTTCTTCAG+AGG + chr2_2:66866873-66866892 Msa0266380:CDS 35.0%
TAAAAGAACGGCTGAACAAA+AGG + chr2_2:66867024-66867043 Msa0266380:CDS 35.0%
GCATTCCTTGAAGAAGTTAT+TGG - chr2_2:66867067-66867086 None:intergenic 35.0%
CTTCTTCAAGGAATGCAAAA+TGG + chr2_2:66867071-66867090 Msa0266380:CDS 35.0%
TCATACTTGATAGGACGAAA+AGG - chr2_2:66867252-66867271 None:intergenic 35.0%
AACAATAACCTAAACGGTGT+TGG - chr2_2:66867439-66867458 None:intergenic 35.0%
TGAAAATCATATAACCTCCC+CGG + chr2_2:66868286-66868305 Msa0266380:intron 35.0%
CTATTACCTCTCGAACAAAT+TGG + chr2_2:66868347-66868366 Msa0266380:intron 35.0%
TCATCAATGACGAATTGTTC+AGG + chr2_2:66868390-66868409 Msa0266380:intron 35.0%
! GTTAACACTTCGGTTCTAAT+GGG - chr2_2:66868503-66868522 None:intergenic 35.0%
ACCACTAATTTGACATCTGA+AGG + chr2_2:66868545-66868564 Msa0266380:intron 35.0%
TCCTTCAGATGTCAAATTAG+TGG - chr2_2:66868549-66868568 None:intergenic 35.0%
! GTTTTACAATGTCGAATTGC+AGG + chr2_2:66868603-66868622 Msa0266380:intron 35.0%
TTTACAATGTCGAATTGCAG+GGG + chr2_2:66868605-66868624 Msa0266380:intron 35.0%
AAAATTAGAATCACCACACC+AGG - chr2_2:66868632-66868651 None:intergenic 35.0%
AATGGTTGAGATGCATGAAA+TGG + chr2_2:66868685-66868704 Msa0266380:CDS 35.0%
ATGAAATGGTTCCAGGAAAA+CGG + chr2_2:66868699-66868718 Msa0266380:CDS 35.0%
TTTGACAGATACCATGAGTT+GGG + chr2_2:66868722-66868741 Msa0266380:CDS 35.0%
ACTTCACATATCAACTGTTG+TGG - chr2_2:66868791-66868810 None:intergenic 35.0%
! CAACAGTTGATATGTGAAGT+TGG + chr2_2:66868791-66868810 Msa0266380:CDS 35.0%
GGCAAGAATAAAATGAACAG+AGG - chr2_2:66868930-66868949 None:intergenic 35.0%
!! GGTTGATGATTGATACTGAA+TGG - chr2_2:66869037-66869056 None:intergenic 35.0%
TAGCGACCAATTCTTCAATT+TGG + chr2_2:66869173-66869192 Msa0266380:intron 35.0%
AGTTATCAAGTACCTTACCT+TGG + chr2_2:66869356-66869375 Msa0266380:intron 35.0%
GTCAAATTGGTCACAATATG+TGG - chr2_2:66869702-66869721 None:intergenic 35.0%
! TGTGACCAATTTGACGTTTT+AGG + chr2_2:66869707-66869726 Msa0266380:intron 35.0%
AGACACCTAAAACGTCAAAT+TGG - chr2_2:66869715-66869734 None:intergenic 35.0%
TAAGATCAGGGTTCAAACAT+CGG + chr2_2:66869937-66869956 Msa0266380:intron 35.0%
GTAGTTACTCAACAATTGCA+TGG + chr2_2:66870043-66870062 Msa0266380:intron 35.0%
ATGGGTGGCTTCATTAAAAA+AGG + chr2_2:66870062-66870081 Msa0266380:CDS 35.0%
TGGGTGGCTTCATTAAAAAA+GGG + chr2_2:66870063-66870082 Msa0266380:CDS 35.0%
AATAAAACTCCTCTCCACAT+TGG - chr2_2:66870255-66870274 None:intergenic 35.0%
TATTTCCCTGTTGCTCTTAT+TGG + chr2_2:66870300-66870319 Msa0266380:CDS 35.0%
ATTTCCCTGTTGCTCTTATT+GGG + chr2_2:66870301-66870320 Msa0266380:CDS 35.0%
AATACCCAATAAGAGCAACA+GGG - chr2_2:66870308-66870327 None:intergenic 35.0%
!! CTTATTGGGTATTGGATGTT+TGG + chr2_2:66870315-66870334 Msa0266380:CDS 35.0%
TCATGTTATAGGAAGCTATC+AGG + chr2_2:66870419-66870438 Msa0266380:CDS 35.0%
AAGCTATCAGGTATGTATGA+TGG + chr2_2:66870431-66870450 Msa0266380:intron 35.0%
GTCTCAATCATGTCAAAAAC+AGG - chr2_2:66870552-66870571 None:intergenic 35.0%
AACAACAAATCTTAGGAGTC+GGG - chr2_2:66870607-66870626 None:intergenic 35.0%
GAACAACAAATCTTAGGAGT+CGG - chr2_2:66870608-66870627 None:intergenic 35.0%
CATTGCGAACAACAAATCTT+AGG - chr2_2:66870614-66870633 None:intergenic 35.0%
GTTGTTCGCAATGTTTATGT+TGG + chr2_2:66870621-66870640 Msa0266380:CDS 35.0%
TTCAGCATTCACAATGTTTG+TGG + chr2_2:66870875-66870894 Msa0266380:intron 35.0%
TCAGCATTCACAATGTTTGT+GGG + chr2_2:66870876-66870895 Msa0266380:intron 35.0%
! CTATTGGTGACAGAATCATA+AGG - chr2_2:66871271-66871290 None:intergenic 35.0%
CTTATGATTCTGTCACCAAT+AGG + chr2_2:66871269-66871288 Msa0266380:CDS 35.0%
TCATGGATGCATGTGTATAA+TGG + chr2_2:66871348-66871367 Msa0266380:three_prime_UTR 35.0%
!!! TTTTGTAAGGGTGCTTTGAT+TGG + chr2_2:66871425-66871444 Msa0266380:three_prime_UTR 35.0%
GATTGGAACAAATGGAGATA+AGG + chr2_2:66871442-66871461 Msa0266380:three_prime_UTR 35.0%
TCAAATTGTTTGCAATGGAG+GGG + chr2_2:66871476-66871495 Msa0266380:three_prime_UTR 35.0%
GGCTTCCAATAACTTCTTCA+AGG + chr2_2:66867059-66867078 Msa0266380:CDS 40.0%
CTTCAAGGAATGCAAAATGG+TGG + chr2_2:66867074-66867093 Msa0266380:CDS 40.0%
! TTTTCACAACGTTACTGCCA+TGG + chr2_2:66867105-66867124 Msa0266380:CDS 40.0%
TTGACAAACCAACACCGTTT+AGG + chr2_2:66867428-66867447 Msa0266380:intron 40.0%
AAGAAGACAACGATCTTCGT+TGG + chr2_2:66867543-66867562 Msa0266380:intron 40.0%
GAAAATCATATAACCTCCCC+GGG + chr2_2:66868287-66868306 Msa0266380:intron 40.0%
AAAATCATATAACCTCCCCG+GGG + chr2_2:66868288-66868307 Msa0266380:intron 40.0%
AGACGAAATTTAAATCCCCC+GGG - chr2_2:66868307-66868326 None:intergenic 40.0%
GAGACGAAATTTAAATCCCC+CGG - chr2_2:66868308-66868327 None:intergenic 40.0%
GTTTGGTCATAAGGGAAGTA+AGG - chr2_2:66868458-66868477 None:intergenic 40.0%
AATCCGGAGTTTGGTCATAA+GGG - chr2_2:66868466-66868485 None:intergenic 40.0%
TAATCCGGAGTTTGGTCATA+AGG - chr2_2:66868467-66868486 None:intergenic 40.0%
GGAAAGAAGTCCTGATAATC+CGG - chr2_2:66868482-66868501 None:intergenic 40.0%
! CGTTAACACTTCGGTTCTAA+TGG - chr2_2:66868504-66868523 None:intergenic 40.0%
TAATCACCCTCTACACTCTA+TGG + chr2_2:66868660-66868679 Msa0266380:CDS 40.0%
CATTTGCCATAGAGTGTAGA+GGG - chr2_2:66868669-66868688 None:intergenic 40.0%
! TATCTGTCAAACCGTTTTCC+TGG - chr2_2:66868713-66868732 None:intergenic 40.0%
GTTTGACAGATACCATGAGT+TGG + chr2_2:66868721-66868740 Msa0266380:CDS 40.0%
! GAAGCATTTGGAGAAAAGCT+TGG + chr2_2:66868749-66868768 Msa0266380:CDS 40.0%
!! GTTGATATGTGAAGTTGGTG+TGG + chr2_2:66868796-66868815 Msa0266380:CDS 40.0%
TGGTGTGGACATTGTTTACA+TGG + chr2_2:66868811-66868830 Msa0266380:CDS 40.0%
GACATTGTTTACATGGTCAC+CGG + chr2_2:66868818-66868837 Msa0266380:CDS 40.0%
ACATTGTTTACATGGTCACC+GGG + chr2_2:66868819-66868838 Msa0266380:CDS 40.0%
TTATGAGAGAGCCAAACTCA+TGG + chr2_2:66869290-66869309 Msa0266380:intron 40.0%
ACCATTGGTATCCATGAGTT+TGG - chr2_2:66869304-66869323 None:intergenic 40.0%
TTATTGAGAATGGGTCGTGA+TGG - chr2_2:66869542-66869561 None:intergenic 40.0%
TTACTCAACAATTGCATGGG+TGG + chr2_2:66870047-66870066 Msa0266380:CDS 40.0%
CAATACCCAATAAGAGCAAC+AGG - chr2_2:66870309-66870328 None:intergenic 40.0%
CTGTTGCTCTTATTGGGTAT+TGG + chr2_2:66870307-66870326 Msa0266380:CDS 40.0%
TGACATGATTGAGACCGTAA+TGG + chr2_2:66870557-66870576 Msa0266380:CDS 40.0%
!! GGTGAGCACCTTTCTAAAAT+TGG + chr2_2:66870642-66870661 Msa0266380:intron 40.0%
!! ATCCGATGATGGAGATTTCA+TGG + chr2_2:66870772-66870791 Msa0266380:intron 40.0%
!! TCCGATGATGGAGATTTCAT+GGG + chr2_2:66870773-66870792 Msa0266380:intron 40.0%
ACCCATGAAATCTCCATCAT+CGG - chr2_2:66870777-66870796 None:intergenic 40.0%
GGAATAACCTTCCCATTCTT+TGG + chr2_2:66870897-66870916 Msa0266380:CDS 40.0%
TCCTCCAAAAAATCCAAGGA+GGG - chr2_2:66870925-66870944 None:intergenic 40.0%
ATCCTCCAAAAAATCCAAGG+AGG - chr2_2:66870926-66870945 None:intergenic 40.0%
CCAATCCTCCAAAAAATCCA+AGG - chr2_2:66870929-66870948 None:intergenic 40.0%
!!! CCTTGGATTTTTTGGAGGAT+TGG + chr2_2:66870926-66870945 Msa0266380:CDS 40.0%
TGTAGCTTCCTTGCATTATG+TGG + chr2_2:66871070-66871089 Msa0266380:intron 40.0%
ATAGCAAGCCACATAATGCA+AGG - chr2_2:66871081-66871100 None:intergenic 40.0%
CAAGACAAGCTAAACCTCTT+AGG - chr2_2:66871111-66871130 None:intergenic 40.0%
!! CTAAGAGGTTTAGCTTGTCT+TGG + chr2_2:66871109-66871128 Msa0266380:CDS 40.0%
CTTGTCTTGGTTCACAAACT+GGG + chr2_2:66871122-66871141 Msa0266380:CDS 40.0%
! TTGGTGACAGAATCATAAGG+AGG - chr2_2:66871268-66871287 None:intergenic 40.0%
ATGATTCTGTCACCAATAGG+TGG + chr2_2:66871272-66871291 Msa0266380:CDS 40.0%
! ATTAGCCTCAATCCACCTAT+TGG - chr2_2:66871287-66871306 None:intergenic 40.0%
ATACTCAATGCCAAGTCCTA+CGG + chr2_2:66871308-66871327 Msa0266380:CDS 40.0%
AGTATAGAAACCGTAGGACT+TGG - chr2_2:66871321-66871340 None:intergenic 40.0%
TCGTCAAGTATAGAAACCGT+AGG - chr2_2:66871327-66871346 None:intergenic 40.0%
ATGACAAAGGCAGGTCTAAA+AGG - chr2_2:66871379-66871398 None:intergenic 40.0%
ATGCAAGATATGACAAAGGC+AGG - chr2_2:66871388-66871407 None:intergenic 40.0%
GGTGCTTTGATTGGAACAAA+TGG + chr2_2:66871434-66871453 Msa0266380:three_prime_UTR 40.0%
! TCAGCCTCTGAAGAAGCAAT+TGG - chr2_2:66866880-66866899 None:intergenic 45.0%
TGCTTCTTCAGAGGCTGAAA+AGG + chr2_2:66866882-66866901 Msa0266380:CDS 45.0%
TTTCTCAGCACAAGAACCAG+AGG + chr2_2:66866909-66866928 Msa0266380:CDS 45.0%
ATACCATCCAAGACTTGCCA+TGG - chr2_2:66867161-66867180 None:intergenic 45.0%
AAATCATATAACCTCCCCGG+GGG + chr2_2:66868289-66868308 Msa0266380:intron 45.0%
GACGAAATTTAAATCCCCCG+GGG - chr2_2:66868306-66868325 None:intergenic 45.0%
CTTCCCTTATGACCAAACTC+CGG + chr2_2:66868460-66868479 Msa0266380:intron 45.0%
TGACCAAACTCCGGATTATC+AGG + chr2_2:66868469-66868488 Msa0266380:intron 45.0%
AGTCCTGATAATCCGGAGTT+TGG - chr2_2:66868475-66868494 None:intergenic 45.0%
CCATTTGCCATAGAGTGTAG+AGG - chr2_2:66868670-66868689 None:intergenic 45.0%
CCTCTACACTCTATGGCAAA+TGG + chr2_2:66868667-66868686 Msa0266380:CDS 45.0%
GAGATGCATGAAATGGTTCC+AGG + chr2_2:66868692-66868711 Msa0266380:CDS 45.0%
ATGCTTCTTGTCCCAACTCA+TGG - chr2_2:66868736-66868755 None:intergenic 45.0%
!! GAGTTGGGACAAGAAGCATT+TGG + chr2_2:66868737-66868756 Msa0266380:CDS 45.0%
TTGGAGAAAAGCTTGGACTC+TGG + chr2_2:66868756-66868775 Msa0266380:CDS 45.0%
CATTGTTTACATGGTCACCG+GGG + chr2_2:66868820-66868839 Msa0266380:CDS 45.0%
ATTGTTTACATGGTCACCGG+GGG + chr2_2:66868821-66868840 Msa0266380:CDS 45.0%
! TTTCTGCAGTGATTTTCCCC+CGG - chr2_2:66868840-66868859 None:intergenic 45.0%
GGAGTTGAAGTTGGGAAGAT+GGG - chr2_2:66868951-66868970 None:intergenic 45.0%
TGGAGTTGAAGTTGGGAAGA+TGG - chr2_2:66868952-66868971 None:intergenic 45.0%
CCAGCAATGGAGTTGAAGTT+GGG - chr2_2:66868959-66868978 None:intergenic 45.0%
ACCAGCAATGGAGTTGAAGT+TGG - chr2_2:66868960-66868979 None:intergenic 45.0%
CTGCAGCAATCATGTCTTTG+AGG + chr2_2:66868990-66869009 Msa0266380:CDS 45.0%
GCCAAACTCATGGATACCAA+TGG + chr2_2:66869300-66869319 Msa0266380:intron 45.0%
ACCTTACCTTGGATGATCGA+AGG + chr2_2:66869367-66869386 Msa0266380:intron 45.0%
TCCTTCGATCATCCAAGGTA+AGG - chr2_2:66869371-66869390 None:intergenic 45.0%
ATGGATCCTTCGATCATCCA+AGG - chr2_2:66869376-66869395 None:intergenic 45.0%
ACACGTTGCTCATCAGTAAG+AGG - chr2_2:66869439-66869458 None:intergenic 45.0%
AAAGGGAGTTCAACCAGATG+TGG + chr2_2:66870080-66870099 Msa0266380:CDS 45.0%
!! AGTTGTGGCTTTGTAGCCAT+AGG - chr2_2:66870106-66870125 None:intergenic 45.0%
TACAAAGCCACAACTACACC+AGG + chr2_2:66870111-66870130 Msa0266380:CDS 45.0%
AACAGTTCCTGGTGTAGTTG+TGG - chr2_2:66870121-66870140 None:intergenic 45.0%
GATGTTGCATTTGCCTATGC+CGG + chr2_2:66870165-66870184 Msa0266380:CDS 45.0%
GCTGCAACTATAAGCCAAGT+AGG - chr2_2:66870381-66870400 None:intergenic 45.0%
ACAAATCTTAGGAGTCGGGT+TGG - chr2_2:66870603-66870622 None:intergenic 45.0%
GCTTGTCTTGGTTCACAAAC+TGG + chr2_2:66871121-66871140 Msa0266380:CDS 45.0%
GTGACAGAATCATAAGGAGG+AGG - chr2_2:66871265-66871284 None:intergenic 45.0%
!! TGTCACCAATAGGTGGATTG+AGG + chr2_2:66871279-66871298 Msa0266380:CDS 45.0%
AATTGTTTGCAATGGAGGGG+AGG + chr2_2:66871479-66871498 Msa0266380:three_prime_UTR 45.0%
ATTGTTTGCAATGGAGGGGA+GGG + chr2_2:66871480-66871499 Msa0266380:three_prime_UTR 45.0%
!! AAAAATATAAATAAAAAAAG+TGG + chr2_2:66867663-66867682 Msa0266380:intron 5.0%
!!! ATTTTTTTAAAAAAAAACTA+AGG + chr2_2:66868224-66868243 Msa0266380:intron 5.0%
!!! TTATTTTATATATTAATTTC+TGG - chr2_2:66869834-66869853 None:intergenic 5.0%
!!! TTAAAGAAAAATAAATTTTA+TGG - chr2_2:66870981-66871000 None:intergenic 5.0%
!!! TATTTTTCTTTAAATATATT+TGG + chr2_2:66870988-66871007 Msa0266380:intron 5.0%
TGTTGGAGACTCAGTTCCCA+TGG - chr2_2:66866848-66866867 None:intergenic 50.0%
CACAACGTTACTGCCATGGT+TGG + chr2_2:66867109-66867128 Msa0266380:CDS 50.0%
CTTGAGTCTTCCATCTGCCA+TGG + chr2_2:66867141-66867160 Msa0266380:CDS 50.0%
GAAATTTAAATCCCCCGGGG+AGG - chr2_2:66868303-66868322 None:intergenic 50.0%
! CTGAGCCCAATTTGTTCGAG+AGG - chr2_2:66868356-66868375 None:intergenic 50.0%
CTCTCGAACAAATTGGGCTC+AGG + chr2_2:66868354-66868373 Msa0266380:intron 50.0%
TCGAACAAATTGGGCTCAGG+TGG + chr2_2:66868357-66868376 Msa0266380:intron 50.0%
CCCAACTTCAACTCCATTGC+TGG + chr2_2:66868956-66868975 Msa0266380:CDS 50.0%
CTCCATTGCTGGTATCTCCT+TGG + chr2_2:66868967-66868986 Msa0266380:CDS 50.0%
AGCCAAGGAGATACCAGCAA+TGG - chr2_2:66868972-66868991 None:intergenic 50.0%
AGACATGATTGCTGCAGCCA+AGG - chr2_2:66868987-66869006 None:intergenic 50.0%
!! GAGAATGGGTCGTGATGGAT+TGG - chr2_2:66869537-66869556 None:intergenic 50.0%
GTTCAACCAGATGTGGCCTA+TGG + chr2_2:66870087-66870106 Msa0266380:CDS 50.0%
TTGTAGCCATAGGCCACATC+TGG - chr2_2:66870096-66870115 None:intergenic 50.0%
CTCCAACACCACATTGTGTC+CGG - chr2_2:66870187-66870206 None:intergenic 50.0%
ACACCGGAGAAACCATCCAA+GGG + chr2_2:66870228-66870247 Msa0266380:CDS 50.0%
AACCATCCAAGGGTCCAATG+TGG + chr2_2:66870238-66870257 Msa0266380:CDS 50.0%
!! CAAGGAGGGCACCAAAGAAT+GGG - chr2_2:66870911-66870930 None:intergenic 50.0%
!!! GGTGCCCTCCTTGGATTTTT+TGG + chr2_2:66870918-66870937 Msa0266380:CDS 50.0%
!!! GCCCTCCTTGGATTTTTTGG+AGG + chr2_2:66870921-66870940 Msa0266380:CDS 50.0%
TGGAGACTCAGTTCCCATGG+TGG - chr2_2:66866845-66866864 None:intergenic 55.0%
GTTACTGCCATGGTTGGAGC+TGG + chr2_2:66867115-66867134 Msa0266380:CDS 55.0%
CAAGACACCAGCTCCAACCA+TGG - chr2_2:66867125-66867144 None:intergenic 55.0%
CCAAGACTTGCCATGGCAGA+TGG - chr2_2:66867154-66867173 None:intergenic 55.0%
CCATCTGCCATGGCAAGTCT+TGG + chr2_2:66867151-66867170 Msa0266380:CDS 55.0%
CTGCCATGGCAAGTCTTGGA+TGG + chr2_2:66867155-66867174 Msa0266380:CDS 55.0%
ATGTCGAATTGCAGGGGTCC+TGG + chr2_2:66868611-66868630 Msa0266380:intron 55.0%
TGCCTATGCCGGACACAATG+TGG + chr2_2:66870176-66870195 Msa0266380:CDS 55.0%
CACCACATTGTGTCCGGCAT+AGG - chr2_2:66870181-66870200 None:intergenic 55.0%
TGCCGGACACAATGTGGTGT+TGG + chr2_2:66870182-66870201 Msa0266380:CDS 55.0%
AGCAACAATCCCGTCCACAC+CGG + chr2_2:66870212-66870231 Msa0266380:CDS 55.0%
ATGGTTTCTCCGGTGTGGAC+GGG - chr2_2:66870224-66870243 None:intergenic 55.0%
GATGGTTTCTCCGGTGTGGA+CGG - chr2_2:66870225-66870244 None:intergenic 55.0%
CTTGGATGGTTTCTCCGGTG+TGG - chr2_2:66870229-66870248 None:intergenic 55.0%
CACACCGGAGAAACCATCCA+AGG + chr2_2:66870227-66870246 Msa0266380:CDS 55.0%
GGACCCTTGGATGGTTTCTC+CGG - chr2_2:66870234-66870253 None:intergenic 55.0%
CTCCACATTGGACCCTTGGA+TGG - chr2_2:66870243-66870262 None:intergenic 55.0%
TCCAAGGGTCCAATGTGGAG+AGG + chr2_2:66870243-66870262 Msa0266380:CDS 55.0%
TCCTCTCCACATTGGACCCT+TGG - chr2_2:66870247-66870266 None:intergenic 55.0%
!! GAGGGCACCAAAGAATGGGA+AGG - chr2_2:66870907-66870926 None:intergenic 55.0%
!! CCAAGGAGGGCACCAAAGAA+TGG - chr2_2:66870912-66870931 None:intergenic 55.0%
!! CCATTCTTTGGTGCCCTCCT+TGG + chr2_2:66870909-66870928 Msa0266380:CDS 55.0%
GAATTGCAGGGGTCCTGGTG+TGG + chr2_2:66868616-66868635 Msa0266380:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 66866705 66871672 66866705 ID=Msa0266380;Name=Msa0266380
chr2_2 mRNA 66866705 66871672 66866705 ID=Msa0266380-mRNA-1;Parent=Msa0266380;Name=Msa0266380-mRNA-1;_AED=0.08;_eAED=0.08;_QI=142|1|1|1|1|1|8|329|462
chr2_2 exon 66866705 66866930 66866705 ID=Msa0266380-mRNA-1:exon:21252;Parent=Msa0266380-mRNA-1
chr2_2 exon 66867019 66867176 66867019 ID=Msa0266380-mRNA-1:exon:21253;Parent=Msa0266380-mRNA-1
chr2_2 exon 66868625 66869011 66868625 ID=Msa0266380-mRNA-1:exon:21254;Parent=Msa0266380-mRNA-1
chr2_2 exon 66870047 66870440 66870047 ID=Msa0266380-mRNA-1:exon:21255;Parent=Msa0266380-mRNA-1
chr2_2 exon 66870537 66870642 66870537 ID=Msa0266380-mRNA-1:exon:21256;Parent=Msa0266380-mRNA-1
chr2_2 exon 66870880 66870971 66870880 ID=Msa0266380-mRNA-1:exon:21257;Parent=Msa0266380-mRNA-1
chr2_2 exon 66871075 66871143 66871075 ID=Msa0266380-mRNA-1:exon:21258;Parent=Msa0266380-mRNA-1
chr2_2 exon 66871245 66871672 66871245 ID=Msa0266380-mRNA-1:exon:21259;Parent=Msa0266380-mRNA-1
chr2_2 five_prime_UTR 66866705 66866846 66866705 ID=Msa0266380-mRNA-1:five_prime_utr;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66866847 66866930 66866847 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66867019 66867176 66867019 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66868625 66869011 66868625 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66870047 66870440 66870047 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66870537 66870642 66870537 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66870880 66870971 66870880 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66871075 66871143 66871075 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 CDS 66871245 66871343 66871245 ID=Msa0266380-mRNA-1:cds;Parent=Msa0266380-mRNA-1
chr2_2 three_prime_UTR 66871344 66871672 66871344 ID=Msa0266380-mRNA-1:three_prime_utr;Parent=Msa0266380-mRNA-1
Gene Sequence

>Msa0266380

ATGGGAACTGAGTCTCCAACAAATGAAAAACCAATTGCTTCTTCAGAGGCTGAAAAGGTCTCTTTCTCAGCACAAGAACCAGAGAGAGATAAAAGAACGGCTGAACAAAAGGCAATTGATGATTGGCTTCCAATAACTTCTTCAAGGAATGCAAAATGGTGGTACGCAGCTTTTCACAACGTTACTGCCATGGTTGGAGCTGGTGTCTTGAGTCTTCCATCTGCCATGGCAAGTCTTGGATGGGGTCCTGGTGTGGTGATTCTAATTTTATCATGGATAATCACCCTCTACACTCTATGGCAAATGGTTGAGATGCATGAAATGGTTCCAGGAAAACGGTTTGACAGATACCATGAGTTGGGACAAGAAGCATTTGGAGAAAAGCTTGGACTCTGGATTGTCGTGCCACAACAGTTGATATGTGAAGTTGGTGTGGACATTGTTTACATGGTCACCGGGGGAAAATCACTGCAGAAAATTCATGATTTGGTGTGTAAAAAAAATTGCAAATCAATGAAAACTACTTATTTCATCATGATATTTGCCTCTGTTCATTTTATTCTTGCCCATCTTCCCAACTTCAACTCCATTGCTGGTATCTCCTTGGCTGCAGCAATCATGTCTTTGAGTTACTCAACAATTGCATGGGTGGCTTCATTAAAAAAGGGAGTTCAACCAGATGTGGCCTATGGCTACAAAGCCACAACTACACCAGGAACTGTTTTCAATTTCTTCAGTGCTTTAGGTGATGTTGCATTTGCCTATGCCGGACACAATGTGGTGTTGGAGATTCAAGCAACAATCCCGTCCACACCGGAGAAACCATCCAAGGGTCCAATGTGGAGAGGAGTTTTATTAGCATATATTGTTGTAGCTTTGTGTTATTTCCCTGTTGCTCTTATTGGGTATTGGATGTTTGGCAATTCAGTTGCTGATAATATTCTTACTTCTCTTAATAAACCTACTTGGCTTATAGTTGCAGCTAACATGTTTGTTGTCATTCATGTTATAGGAAGCTATCAGTTGTATGCAATGCCTGTTTTTGACATGATTGAGACCGTAATGGTCAAAAAATTGCGTTTCAAGCCAACCCGACTCCTAAGATTTGTTGTTCGCAATGTTTATGTTGCATTCACAATGTTTGTGGGAATAACCTTCCCATTCTTTGGTGCCCTCCTTGGATTTTTTGGAGGATTGGCATTTGCTCCCACAACATATTTTCTTCCTTGCATTATGTGGCTTGCTATTTACAAACCTAAGAGGTTTAGCTTGTCTTGGTTCACAAACTGGATTTGCATTATACTTGGCCTCCTCCTTATGATTCTGTCACCAATAGGTGGATTGAGGCTAATCATACTCAATGCCAAGTCCTACGGTTTCTATACTTGA
Protein sequence

>Msa0266380

MGTESPTNEKPIASSEAEKVSFSAQEPERDKRTAEQKAIDDWLPITSSRNAKWWYAAFHNVTAMVGAGVLSLPSAMASLGWGPGVVILILSWIITLYTLWQMVEMHEMVPGKRFDRYHELGQEAFGEKLGLWIVVPQQLICEVGVDIVYMVTGGKSLQKIHDLVCKKNCKSMKTTYFIMIFASVHFILAHLPNFNSIAGISLAAAIMSLSYSTIAWVASLKKGVQPDVAYGYKATTTPGTVFNFFSALGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWRGVLLAYIVVALCYFPVALIGYWMFGNSVADNILTSLNKPTWLIVAANMFVVIHVIGSYQLYAMPVFDMIETVMVKKLRFKPTRLLRFVVRNVYVAFTMFVGITFPFFGALLGFFGGLAFAPTTYFLPCIMWLAIYKPKRFSLSWFTNWICIILGLLLMILSPIGGLRLIILNAKSYGFYT*