AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0341860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0341860 MtrunA17_Chr2g0331721 98.918 462 4 1 1 462 1 461 0.0 928
Msa0341860 MtrunA17_Chr7g0230011 80.087 462 73 2 1 462 1 443 0.0 723
Msa0341860 MtrunA17_Chr6g0458931 74.348 460 100 3 3 462 14 455 0.0 722
Msa0341860 MtrunA17_Chr6g0459021 74.943 435 108 1 28 462 14 447 0.0 657
Msa0341860 MtrunA17_Chr6g0459001 70.042 474 100 2 30 462 4 476 0.0 648
Msa0341860 MtrunA17_Chr8g0376991 67.586 435 140 1 28 462 3 436 0.0 639
Msa0341860 MtrunA17_Chr6g0458921 74.359 429 106 3 35 462 3 428 0.0 628
Msa0341860 MtrunA17_Chr3g0122921 66.895 438 126 2 25 462 10 428 0.0 619
Msa0341860 MtrunA17_Chr6g0459031 72.430 428 116 2 36 462 2 428 0.0 619
Msa0341860 MtrunA17_Chr4g0057851 67.554 413 133 1 48 460 10 421 0.0 606
Msa0341860 MtrunA17_Chr2g0328081 73.598 428 111 2 36 462 2 428 0.0 599
Msa0341860 MtrunA17_Chr6g0459011 76.142 394 93 1 64 457 1 393 0.0 585
Msa0341860 MtrunA17_Chr5g0422741 60.948 443 168 2 20 462 2 439 0.0 547
Msa0341860 MtrunA17_Chr1g0198531 59.862 436 169 3 27 462 8 437 0.0 530
Msa0341860 MtrunA17_Chr1g0198541 58.891 433 173 2 30 462 10 437 0.0 520
Msa0341860 MtrunA17_Chr3g0077651 52.874 435 202 2 31 462 35 469 4.89e-163 468
Msa0341860 MtrunA17_Chr3g0143701 36.797 462 270 9 4 448 39 495 7.53e-97 300
Msa0341860 MtrunA17_Chr5g0406331 37.061 456 263 8 3 439 37 487 2.16e-96 299
Msa0341860 MtrunA17_Chr2g0281341 37.269 432 260 5 40 461 87 517 8.98e-96 298
Msa0341860 MtrunA17_Chr4g0038711 35.332 467 272 7 5 449 29 487 5.60e-92 288
Msa0341860 MtrunA17_Chr5g0406351 35.794 447 274 6 28 462 87 532 6.72e-92 288
Msa0341860 MtrunA17_Chr3g0140761 30.595 487 286 13 12 458 1 475 9.46e-60 203
Msa0341860 MtrunA17_Chr1g0146491 30.687 466 269 12 35 458 12 465 2.86e-55 191
Msa0341860 MtrunA17_Chr1g0198081 28.782 476 310 12 1 462 19 479 1.66e-54 189
Msa0341860 MtrunA17_Chr4g0027241 28.211 475 311 11 1 461 1 459 5.93e-54 187
Msa0341860 MtrunA17_Chr8g0387941 28.194 454 302 10 24 461 2 447 9.50e-54 186
Msa0341860 MtrunA17_Chr3g0125091 28.542 480 285 12 28 458 3 473 1.29e-51 181
Msa0341860 MtrunA17_Chr3g0125101 29.424 469 281 12 26 458 19 473 4.83e-51 180
Msa0341860 MtrunA17_Chr3g0125081 29.095 464 283 12 31 458 19 472 6.45e-50 177
Msa0341860 MtrunA17_Chr1g0206871 28.446 457 272 10 49 458 16 464 8.08e-50 176
Msa0341860 MtrunA17_Chr3g0103271 28.721 477 285 13 23 457 2 465 2.20e-48 172
Msa0341860 MtrunA17_Chr4g0037201 26.994 489 302 11 10 458 32 505 4.43e-48 172
Msa0341860 MtrunA17_Chr3g0130641 30.435 460 262 12 40 458 28 470 6.29e-48 171
Msa0341860 MtrunA17_Chr1g0159761 30.134 448 261 13 25 433 5 439 4.77e-46 166
Msa0341860 MtrunA17_Chr5g0447221 26.884 491 290 12 1 452 1 461 7.84e-45 162
Msa0341860 MtrunA17_Chr3g0130651 28.571 441 267 11 21 428 6 431 8.08e-45 162
Msa0341860 MtrunA17_Chr1g0146411 28.702 439 266 14 26 427 7 435 1.52e-43 159
Msa0341860 MtrunA17_Chr1g0146391 27.623 467 285 14 35 462 2 454 2.96e-43 158
Msa0341860 MtrunA17_Chr8g0384661 27.640 445 279 10 49 458 51 487 4.54e-43 158
Msa0341860 MtrunA17_Chr1g0184721 26.049 453 294 13 30 461 5 437 3.26e-42 155
Msa0341860 MtrunA17_Chr3g0103231 27.212 452 291 10 33 452 10 455 6.52e-42 154
Msa0341860 MtrunA17_Chr3g0140541 26.697 442 274 11 47 449 24 454 1.27e-41 154
Msa0341860 MtrunA17_Chr5g0447231 27.765 443 271 14 49 452 26 458 3.02e-39 147
Msa0341860 MtrunA17_Chr3g0103211 27.434 452 290 10 33 452 10 455 5.32e-39 146
Msa0341860 MtrunA17_Chr3g0140531 25.996 477 301 16 26 461 2 467 7.91e-37 140
Msa0341860 MtrunA17_Chr1g0146331 25.330 454 279 13 35 443 10 448 2.79e-36 139
Msa0341860 MtrunA17_Chr2g0293331 25.566 442 267 11 49 443 13 439 3.21e-36 139
Msa0341860 MtrunA17_Chr3g0103221 24.766 428 288 9 56 452 38 462 3.52e-35 136
Msa0341860 MtrunA17_Chr4g0058161 24.449 454 324 12 14 461 7 447 4.64e-35 135
Msa0341860 MtrunA17_Chr1g0206851 27.920 351 207 10 143 458 35 374 1.10e-34 133
Msa0341860 MtrunA17_Chr1g0206831 29.355 310 180 8 177 457 37 336 2.38e-33 129
Msa0341860 MtrunA17_Chr5g0436281 29.049 389 258 12 76 458 1 377 6.06e-33 128
Msa0341860 MtrunA17_Chr3g0103251 25.610 328 190 7 177 452 43 368 3.76e-25 106
Msa0341860 MtrunA17_Chr1g0206821 28.899 218 126 6 264 458 313 524 2.09e-23 103
Msa0341860 MtrunA17_Chr1g0206821 32.365 241 134 7 49 267 23 256 2.89e-23 102
Msa0341860 MtrunA17_Chr3g0112971 25.789 380 252 13 94 461 72 433 5.61e-21 95.1
Msa0341860 MtrunA17_Chr5g0402151 29.496 278 162 7 59 329 11 261 9.07e-20 89.0
Msa0341860 MtrunA17_Chr3g0105861 23.684 456 309 15 12 454 1 430 7.97e-17 82.4
Msa0341860 MtrunA17_Chr3g0130661 28.736 174 108 2 301 458 1 174 1.62e-16 77.4
Msa0341860 MtrunA17_Chr7g0217401 24.876 402 252 16 32 417 25 392 4.66e-15 77.0
Msa0341860 MtrunA17_Chr7g0239011 21.127 426 293 15 19 431 32 427 7.79e-15 76.3
Msa0341860 MtrunA17_Chr8g0361631 21.814 408 279 14 26 425 2 377 2.22e-14 74.7
Msa0341860 MtrunA17_Chr7g0217381 24.874 398 249 15 38 416 24 390 7.54e-14 73.2
Msa0341860 MtrunA17_Chr4g0027261 26.702 191 124 4 280 461 1 184 3.10e-13 68.2
Msa0341860 MtrunA17_Chr3g0090101 24.934 377 241 15 58 421 2 349 7.45e-12 67.0
Msa0341860 MtrunA17_Chr3g0130671 28.846 208 125 7 49 241 40 239 1.07e-11 65.5
Msa0341860 MtrunA17_Chr6g0455951 22.199 482 294 19 1 443 3 442 4.67e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0341860 AT5G40780.1 77.753 445 92 2 24 462 3 446 0.0 733
Msa0341860 AT1G48640.1 72.789 441 116 2 25 462 14 453 0.0 672
Msa0341860 AT5G40780.3 79.198 399 82 1 64 462 1 398 0.0 670
Msa0341860 AT5G40780.2 79.198 399 82 1 64 462 1 398 0.0 670
Msa0341860 AT1G24400.1 74.318 440 112 1 23 462 3 441 0.0 664
Msa0341860 AT1G67640.1 74.318 440 111 2 23 462 4 441 0.0 649
Msa0341860 AT1G61270.1 65.734 429 146 1 21 448 6 434 0.0 593
Msa0341860 AT3G01760.1 64.747 434 146 4 29 456 9 441 0.0 586
Msa0341860 AT1G25530.1 60.369 434 166 2 28 461 12 439 0.0 527
Msa0341860 AT1G71680.1 55.902 449 191 3 20 462 1 448 2.62e-168 481
Msa0341860 AT1G71680.2 56.593 364 151 3 20 377 1 363 1.85e-142 412
Msa0341860 AT1G47670.1 37.269 432 260 5 40 461 85 515 2.76e-94 295
Msa0341860 AT4G35180.1 31.778 450 284 8 5 442 19 457 3.09e-73 239
Msa0341860 AT1G10010.1 33.118 465 268 11 30 458 11 468 6.19e-61 206
Msa0341860 AT1G10010.2 33.118 465 268 11 30 458 12 469 7.33e-61 206
Msa0341860 AT4G35180.2 34.304 309 191 3 5 310 19 318 1.27e-60 201
Msa0341860 AT1G08230.2 29.801 453 296 11 24 461 3 448 6.94e-54 187
Msa0341860 AT1G58360.1 30.533 488 282 12 8 458 10 477 3.19e-53 186
Msa0341860 AT5G41800.1 27.570 428 287 8 50 462 28 447 2.22e-50 177
Msa0341860 AT1G77380.1 30.682 440 253 13 32 433 15 440 9.16e-49 174
Msa0341860 AT5G49630.1 29.957 464 278 13 16 438 2 459 5.00e-48 172
Msa0341860 AT5G09220.1 28.571 448 275 11 49 458 46 486 9.21e-48 171
Msa0341860 AT1G44100.1 27.651 481 291 13 23 458 5 473 1.02e-44 163
Msa0341860 AT5G63850.1 27.477 444 285 8 49 458 19 459 4.47e-44 161
Msa0341860 AT5G23810.1 28.774 424 247 11 47 426 24 436 8.37e-40 149
Msa0341860 AT1G08230.3 30.423 355 227 10 120 461 1 348 3.25e-36 137
Msa0341860 AT1G08230.4 31.118 331 208 10 144 461 6 329 1.42e-34 132
Msa0341860 AT1G08230.6 31.118 331 208 10 144 461 6 329 1.42e-34 132
Msa0341860 AT1G08230.5 31.118 331 208 10 144 461 6 329 1.42e-34 132
Msa0341860 AT3G55740.1 26.667 465 302 16 1 458 1 433 2.55e-32 128
Msa0341860 AT2G39890.2 25.000 448 311 14 23 458 2 436 2.93e-30 122
Msa0341860 AT2G39890.1 25.000 448 311 14 23 458 2 436 2.93e-30 122
Msa0341860 AT2G36590.1 26.316 418 283 15 28 434 3 406 4.74e-30 121
Msa0341860 AT3G55740.2 26.992 389 266 12 76 458 1 377 3.94e-29 118
Msa0341860 AT3G55740.3 26.992 389 266 12 76 458 1 377 3.94e-29 118
Msa0341860 AT2G39890.3 25.385 390 271 11 76 458 1 377 9.08e-29 117
Msa0341860 AT5G23810.2 28.528 326 197 9 47 347 24 338 2.44e-26 110
Msa0341860 AT2G36590.2 27.554 323 213 13 28 342 3 312 1.11e-20 93.2
Msa0341860 AT5G15240.1 25.183 409 242 16 31 416 16 383 2.55e-17 84.3
Msa0341860 AT5G23810.4 27.686 242 143 7 47 265 24 256 1.97e-15 76.6
Msa0341860 AT2G39130.2 23.679 473 304 20 6 452 104 545 2.58e-15 78.6
Msa0341860 AT2G39130.1 23.679 473 304 20 6 452 104 545 2.58e-15 78.6
Msa0341860 AT5G02180.1 22.739 387 266 11 43 420 151 513 6.30e-14 74.3
Msa0341860 AT5G02180.2 22.739 387 266 11 43 420 151 513 6.30e-14 74.3
Msa0341860 AT5G23810.5 25.333 225 137 7 25 230 5 217 4.37e-12 66.2
Msa0341860 AT5G23810.3 25.333 225 137 7 25 230 5 217 4.37e-12 66.2

Find 142 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTCCCTGTTGCTCTTAT+TGG 0.168322 2_4:-63802612 Msa0341860:CDS
TTCAATTTCTTCAGTGCTTT+AGG 0.228996 2_4:-63802771 Msa0341860:CDS
GCATTCCTTGAAGAAGTTAT+TGG 0.252163 2_4:+63805842 None:intergenic
GGAATAACCTTCCCATTCTT+TGG 0.269371 2_4:-63802015 Msa0341860:CDS
ATTTCCCTGTTGCTCTTATT+GGG 0.271137 2_4:-63802611 Msa0341860:CDS
CCATTCTTTGGTGCCCTCCT+TGG 0.281653 2_4:-63802003 Msa0341860:CDS
TTTGTTGTCATTCATGTTAT+AGG 0.284943 2_4:-63802504 Msa0341860:CDS
TTTCTATACTTGACGATTCA+TGG 0.294299 2_4:-63801581 Msa0341860:exon
GCTTGTCTTGGTTCACAAAC+TGG 0.295561 2_4:-63801791 Msa0341860:CDS
CTGTTGCTCTTATTGGGTAT+TGG 0.300006 2_4:-63802605 Msa0341860:CDS
CAAGACAAGCTAAACCTCTT+AGG 0.301943 2_4:+63801804 None:intergenic
ACTGCAGAAAATTCATGATT+TGG 0.311368 2_4:-63804056 Msa0341860:CDS
CCCAACTTCAACTCCATTGC+TGG 0.312413 2_4:-63803950 Msa0341860:CDS
CTTATTGGGTATTGGATGTT+TGG 0.317962 2_4:-63802597 Msa0341860:CDS
CTTCTTCAAGGAATGCAAAA+TGG 0.331015 2_4:-63805835 Msa0341860:CDS
AAGAAATTGAAAACAGTTCC+TGG 0.370996 2_4:+63802783 None:intergenic
TAATCACCCTCTACACTCTA+TGG 0.375402 2_4:-63804246 Msa0341860:CDS
CATTGCGAACAACAAATCTT+AGG 0.383519 2_4:+63802301 None:intergenic
CAATACCCAATAAGAGCAAC+AGG 0.389191 2_4:+63802606 None:intergenic
TGCTTCTTCAGAGGCTGAAA+AGG 0.398966 2_4:-63806024 Msa0341860:CDS
TTGTAGCCATAGGCCACATC+TGG 0.403065 2_4:+63802819 None:intergenic
GAGATGCATGAAATGGTTCC+AGG 0.408422 2_4:-63804214 Msa0341860:CDS
TGTAGCTTCCTTGCATTATG+TGG 0.411632 2_4:-63801842 Msa0341860:intron
TTGGAGAAAAGCTTGGACTC+TGG 0.412306 2_4:-63804150 Msa0341860:CDS
AACAAAAGGCAATTGATGAT+TGG 0.412955 2_4:-63805868 Msa0341860:CDS
TCAGCCTCTGAAGAAGCAAT+TGG 0.414783 2_4:+63806029 None:intergenic
TCATGTTATAGGAAGCTATC+AGG 0.416323 2_4:-63802493 Msa0341860:intron
TCCTCTCCACATTGGACCCT+TGG 0.420922 2_4:+63802668 None:intergenic
ATTAGCCTCAATCCACCTAT+TGG 0.422145 2_4:+63801628 None:intergenic
CTCCACATTGGACCCTTGGA+TGG 0.422755 2_4:+63802672 None:intergenic
GGCTTCCAATAACTTCTTCA+AGG 0.425061 2_4:-63805847 Msa0341860:CDS
TCATGGATGCATGTGTATAA+TGG 0.429106 2_4:-63801564 Msa0341860:three_prime_UTR
TTCAAATTGTTTGCAATGGA+GGG 0.431140 2_4:-63801437 Msa0341860:three_prime_UTR
CCATCTGCCATGGCAAGTCT+TGG 0.434758 2_4:-63805755 Msa0341860:CDS
CCAAGGAGGGCACCAAAGAA+TGG 0.436204 2_4:+63802003 None:intergenic
CTAAGAGGTTTAGCTTGTCT+TGG 0.437451 2_4:-63801803 Msa0341860:CDS
GGACCCTTGGATGGTTTCTC+CGG 0.438031 2_4:+63802681 None:intergenic
CTTATGATTCTGTCACCAAT+AGG 0.442214 2_4:-63801643 Msa0341860:CDS
CTTCTCTTAATAAACCTACT+TGG 0.442717 2_4:-63802548 Msa0341860:CDS
GACATTGTTTACATGGTCAC+CGG 0.442865 2_4:-63804088 Msa0341860:CDS
CTCCATTGCTGGTATCTCCT+TGG 0.443891 2_4:-63803939 Msa0341860:CDS
TTTCAGATTTGCATTATACT+TGG 0.449641 2_4:-63801673 Msa0341860:intron
GATGGTTTCTCCGGTGTGGA+CGG 0.451778 2_4:+63802690 None:intergenic
TTCAGCATTCACAATGTTTG+TGG 0.454604 2_4:-63802037 Msa0341860:intron
ATGGTTTCTCCGGTGTGGAC+GGG 0.455777 2_4:+63802691 None:intergenic
GGTGCTTTGATTGGAACAAA+TGG 0.456812 2_4:-63801478 Msa0341860:three_prime_UTR
TAAAAGAACGGCTGAACAAA+AGG 0.459944 2_4:-63805882 Msa0341860:CDS
ACCAGCAATGGAGTTGAAGT+TGG 0.460631 2_4:+63803949 None:intergenic
CTCCAACACCACATTGTGTC+CGG 0.463600 2_4:+63802728 None:intergenic
GATTGGAACAAATGGAGATA+AGG 0.464708 2_4:-63801470 Msa0341860:three_prime_UTR
CTGCAGCAATCATGTCTTTG+AGG 0.471568 2_4:-63803916 Msa0341860:intron
ATGAAATGGTTCCAGGAAAA+CGG 0.476078 2_4:-63804207 Msa0341860:CDS
TGGAGTTGAAGTTGGGAAGA+TGG 0.477812 2_4:+63803957 None:intergenic
ATGTCGAATTGCAGGGGTCC+TGG 0.479412 2_4:-63804295 Msa0341860:intron
AGTATAGAAACCGTAGGACT+TGG 0.487333 2_4:+63801594 None:intergenic
CTTGTCTTGGTTCACAAACT+GGG 0.488155 2_4:-63801790 Msa0341860:intron
GGAGTTGAAGTTGGGAAGAT+GGG 0.488377 2_4:+63803958 None:intergenic
TCAGCATTCACAATGTTTGT+GGG 0.488938 2_4:-63802036 Msa0341860:intron
GGACTTACAAAATATGTTGT+GGG 0.491845 2_4:+63801955 None:intergenic
ATTCAAATTGTTTGCAATGG+AGG 0.493032 2_4:-63801438 Msa0341860:three_prime_UTR
CCAAGACTTGCCATGGCAGA+TGG 0.495959 2_4:+63805755 None:intergenic
CAAGGAGGGCACCAAAGAAT+GGG 0.496713 2_4:+63802004 None:intergenic
CCTCTACACTCTATGGCAAA+TGG 0.497407 2_4:-63804239 Msa0341860:CDS
TTGCTATTTACAAACCTAAG+AGG 0.497836 2_4:-63801818 Msa0341860:CDS
AACAACAAATCTTAGGAGTC+GGG 0.497913 2_4:+63802308 None:intergenic
TATATTCAAATTGTTTGCAA+TGG 0.499086 2_4:-63801441 Msa0341860:three_prime_UTR
TTTGACAGATACCATGAGTT+GGG 0.500029 2_4:-63804184 Msa0341860:CDS
TGACATGATTGAGACCGTAA+TGG 0.504825 2_4:-63802355 Msa0341860:CDS
GAGTTGGGACAAGAAGCATT+TGG 0.508176 2_4:-63804169 Msa0341860:CDS
GAAGCATTTGGAGAAAAGCT+TGG 0.510904 2_4:-63804157 Msa0341860:CDS
TGTTGGAGACTCAGTTCCCA+TGG 0.511962 2_4:+63806061 None:intergenic
TGGACTTACAAAATATGTTG+TGG 0.515751 2_4:+63801954 None:intergenic
CACAACGTTACTGCCATGGT+TGG 0.517108 2_4:-63805797 Msa0341860:CDS
CCAGCAATGGAGTTGAAGTT+GGG 0.520549 2_4:+63803950 None:intergenic
ATGCAAGATATGACAAAGGC+AGG 0.523499 2_4:+63801527 None:intergenic
GTTGTTCGCAATGTTTATGT+TGG 0.530022 2_4:-63802291 Msa0341860:intron
GCTGCAACTATAAGCCAAGT+AGG 0.535757 2_4:+63802534 None:intergenic
TCAAATTGTTTGCAATGGAG+GGG 0.540207 2_4:-63801436 Msa0341860:three_prime_UTR
CTTGAGTCTTCCATCTGCCA+TGG 0.541643 2_4:-63805765 Msa0341860:CDS
ATTGTTTGCAATGGAGGGGA+GGG 0.545992 2_4:-63801432 Msa0341860:three_prime_UTR
ATGCTTCTTGTCCCAACTCA+TGG 0.547647 2_4:+63804173 None:intergenic
GTTCAACCAGATGTGGCCTA+TGG 0.548195 2_4:-63802825 Msa0341860:CDS
GAACAACAAATCTTAGGAGT+CGG 0.552204 2_4:+63802307 None:intergenic
AGTTGTGGCTTTGTAGCCAT+AGG 0.553663 2_4:+63802809 None:intergenic
TGGTGTGGACATTGTTTACA+TGG 0.554021 2_4:-63804095 Msa0341860:CDS
AATGGTTGAGATGCATGAAA+TGG 0.555074 2_4:-63804221 Msa0341860:CDS
AGACATGATTGCTGCAGCCA+AGG 0.555144 2_4:+63803922 None:intergenic
CTGCCATGGCAAGTCTTGGA+TGG 0.557557 2_4:-63805751 Msa0341860:intron
ACATTGTTTACATGGTCACC+GGG 0.558322 2_4:-63804087 Msa0341860:CDS
GTAGTTACTCAACAATTGCA+TGG 0.558837 2_4:-63802869 Msa0341860:intron
GTTTGACAGATACCATGAGT+TGG 0.571800 2_4:-63804185 Msa0341860:CDS
AACAGTTCCTGGTGTAGTTG+TGG 0.571899 2_4:+63802794 None:intergenic
AATTGTTTGCAATGGAGGGG+AGG 0.572605 2_4:-63801433 Msa0341860:three_prime_UTR
AATAAAACTCCTCTCCACAT+TGG 0.575187 2_4:+63802660 None:intergenic
CAACAGTTGATATGTGAAGT+TGG 0.577281 2_4:-63804115 Msa0341860:CDS
ATACTCAATGCCAAGTCCTA+CGG 0.577308 2_4:-63801604 Msa0341860:CDS
TTACTCAACAATTGCATGGG+TGG 0.581664 2_4:-63802865 Msa0341860:CDS
ACAAATCTTAGGAGTCGGGT+TGG 0.583916 2_4:+63802312 None:intergenic
AAAACCAATTGCTTCTTCAG+AGG 0.589072 2_4:-63806033 Msa0341860:CDS
GTTACTGCCATGGTTGGAGC+TGG 0.590710 2_4:-63805791 Msa0341860:CDS
CTTGGATGGTTTCTCCGGTG+TGG 0.592081 2_4:+63802686 None:intergenic
CATTTGCCATAGAGTGTAGA+GGG 0.594361 2_4:+63804240 None:intergenic
CTTGACTTAATAACCACCAT+GGG 0.594664 2_4:-63806077 Msa0341860:exon
CAAGACACCAGCTCCAACCA+TGG 0.596093 2_4:+63805784 None:intergenic
TAGTTACTCAACAATTGCAT+GGG 0.600051 2_4:-63802868 Msa0341860:CDS
TGCCGGACACAATGTGGTGT+TGG 0.600214 2_4:-63802730 Msa0341860:CDS
ATGATTCTGTCACCAATAGG+TGG 0.600362 2_4:-63801640 Msa0341860:CDS
TACAAAGCCACAACTACACC+AGG 0.601821 2_4:-63802801 Msa0341860:CDS
TGTCACCAATAGGTGGATTG+AGG 0.602435 2_4:-63801633 Msa0341860:CDS
GATGTTGCATTTGCCTATGC+CGG 0.602926 2_4:-63802747 Msa0341860:CDS
GTGACAGAATCATAAGGAGG+AGG 0.610650 2_4:+63801650 None:intergenic
AAAGGGAGTTCAACCAGATG+TGG 0.615250 2_4:-63802832 Msa0341860:CDS
CCATTTGCCATAGAGTGTAG+AGG 0.615329 2_4:+63804239 None:intergenic
ATAGCAAGCCACATAATGCA+AGG 0.617411 2_4:+63801834 None:intergenic
AAAATTAGAATCACCACACC+AGG 0.621042 2_4:+63804277 None:intergenic
TTAACAGAGAGATAAAAGAA+CGG 0.624935 2_4:-63805894 Msa0341860:intron
ACTTGACTTAATAACCACCA+TGG 0.625403 2_4:-63806078 Msa0341860:exon
ACTTCACATATCAACTGTTG+TGG 0.626823 2_4:+63804118 None:intergenic
TGGAGACTCAGTTCCCATGG+TGG 0.630497 2_4:+63806064 None:intergenic
ATACCATCCAAGACTTGCCA+TGG 0.631568 2_4:+63805748 None:intergenic
GAATTGCAGGGGTCCTGGTG+TGG 0.635436 2_4:-63804290 Msa0341860:intron
ATTGTTTACATGGTCACCGG+GGG 0.638235 2_4:-63804085 Msa0341860:CDS
CTATTGGTGACAGAATCATA+AGG 0.645120 2_4:+63801644 None:intergenic
TGTTTGTGTCAACACTAGGA+CGG 0.646719 2_4:-63806215 None:intergenic
TCCAAGGGTCCAATGTGGAG+AGG 0.647648 2_4:-63802669 Msa0341860:CDS
CACCACATTGTGTCCGGCAT+AGG 0.647925 2_4:+63802734 None:intergenic
TAAAATGCAAGATATGACAA+AGG 0.648706 2_4:+63801523 None:intergenic
GGCAAGAATAAAATGAACAG+AGG 0.650976 2_4:+63803979 None:intergenic
AGCAACAATCCCGTCCACAC+CGG 0.654369 2_4:-63802700 Msa0341860:CDS
CTTCAAGGAATGCAAAATGG+TGG 0.654515 2_4:-63805832 Msa0341860:CDS
GTTGATATGTGAAGTTGGTG+TGG 0.658649 2_4:-63804110 Msa0341860:CDS
CACACCGGAGAAACCATCCA+AGG 0.663681 2_4:-63802685 Msa0341860:CDS
AGCCAAGGAGATACCAGCAA+TGG 0.663690 2_4:+63803937 None:intergenic
TTTCTCAGCACAAGAACCAG+AGG 0.673739 2_4:-63805997 Msa0341860:intron
CATTGTTTACATGGTCACCG+GGG 0.675950 2_4:-63804086 Msa0341860:CDS
GAGGGCACCAAAGAATGGGA+AGG 0.676285 2_4:+63802008 None:intergenic
TCGTCAAGTATAGAAACCGT+AGG 0.681478 2_4:+63801588 None:intergenic
AACCATCCAAGGGTCCAATG+TGG 0.683222 2_4:-63802674 Msa0341860:CDS
ACACCGGAGAAACCATCCAA+GGG 0.697058 2_4:-63802684 Msa0341860:CDS
TTGGTGACAGAATCATAAGG+AGG 0.701093 2_4:+63801647 None:intergenic
TGCCTATGCCGGACACAATG+TGG 0.720388 2_4:-63802736 Msa0341860:CDS
AATACCCAATAAGAGCAACA+GGG 0.735246 2_4:+63802607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTATATTTTTTAAA+TGG + chr2_4:63802227-63802246 None:intergenic 0.0%
!!! ATTTATATTTTTTAAATGGT+TGG + chr2_4:63802223-63802242 None:intergenic 10.0%
!! ATTTGAATATTAATGAAATA+TGG + chr2_4:63804203-63804222 None:intergenic 10.0%
!!! TATATAAAATAAAAGTACAA+CGG - chr2_4:63804410-63804429 Msa0341860:intron 10.0%
!!! TATGTGTAGTTTAAATTTTT+TGG + chr2_4:63801375-63801394 None:intergenic 15.0%
!! TGTCAAAAAAAAAAAAACAA+GGG + chr2_4:63801982-63802001 None:intergenic 15.0%
!! TTGTCAAAAAAAAAAAAACA+AGG + chr2_4:63801983-63802002 None:intergenic 15.0%
!!! AATTTAACTTCTAGAAAAAA+AGG - chr2_4:63802066-63802085 Msa0341860:intron 15.0%
!! AGAAAATATTTAAGAAAAGA+AGG + chr2_4:63803626-63803645 None:intergenic 15.0%
!! ATAATATTATGTTATACCAT+TGG + chr2_4:63803887-63803906 None:intergenic 15.0%
!!! GAAAATAGATTATTTTTTTG+TGG + chr2_4:63804528-63804547 None:intergenic 15.0%
!!! TATTTTTTAACTAAACTTGA+TGG - chr2_4:63804556-63804575 Msa0341860:intron 15.0%
!!! TAAAATATCCAATTTTAGAA+AGG + chr2_4:63805215-63805234 None:intergenic 15.0%
!! TAAAACATAACTTATCTTAT+TGG - chr2_4:63805768-63805787 Msa0341860:CDS 15.0%
!!! TTTTCTTTACTTTTTTTGTA+AGG - chr2_4:63805974-63805993 Msa0341860:intron 15.0%
!!! TTTCTTTACTTTTTTTGTAA+GGG - chr2_4:63805975-63805994 Msa0341860:intron 15.0%
!!! TGTCAATAAATGTTTTGATA+TGG + chr2_4:63801325-63801344 None:intergenic 20.0%
!!! TTAAATTTTTTGGAACAAAG+AGG + chr2_4:63801365-63801384 None:intergenic 20.0%
!!! AACTAGAATTTTTCAAACAA+GGG + chr2_4:63801874-63801893 None:intergenic 20.0%
!!! AAACTAGAATTTTTCAAACA+AGG + chr2_4:63801875-63801894 None:intergenic 20.0%
!! AGTTTAAACAATAACCTAAA+CGG + chr2_4:63802013-63802032 None:intergenic 20.0%
!! ACTATTCACTTATTAGAAAA+TGG - chr2_4:63802030-63802049 Msa0341860:CDS 20.0%
!! AATAATAATAATACACCCTT+TGG + chr2_4:63802257-63802276 None:intergenic 20.0%
!!! AGTTTTGAAATTTGAGAATT+GGG + chr2_4:63802336-63802355 None:intergenic 20.0%
!!! TAGTTTTGAAATTTGAGAAT+TGG + chr2_4:63802337-63802356 None:intergenic 20.0%
!! TTCTCAAATTTCAAAACTAT+TGG - chr2_4:63802338-63802357 Msa0341860:CDS 20.0%
!! CAATAAACACTTACTTAATT+AGG + chr2_4:63802401-63802420 None:intergenic 20.0%
!! CTAATTAAGTAAGTGTTTAT+TGG - chr2_4:63802399-63802418 Msa0341860:intron 20.0%
!! GTTCATTATGCATTATTTAT+AGG - chr2_4:63802436-63802455 Msa0341860:intron 20.0%
!!! ATCAAGTTTGATTTTAATCA+TGG + chr2_4:63802469-63802488 None:intergenic 20.0%
!! TTTAGTAGTATTTATCAACA+TGG + chr2_4:63803690-63803709 None:intergenic 20.0%
!!! TGTTGATAAATACTACTAAA+AGG - chr2_4:63803690-63803709 Msa0341860:intron 20.0%
!! TATTCAACTTTATTGAGAAT+GGG + chr2_4:63804119-63804138 None:intergenic 20.0%
!! ATATTCAACTTTATTGAGAA+TGG + chr2_4:63804120-63804139 None:intergenic 20.0%
!! TACATAATATATGTAAGATC+AGG - chr2_4:63804486-63804505 Msa0341860:intron 20.0%
!! ACATAATATATGTAAGATCA+GGG - chr2_4:63804487-63804506 Msa0341860:intron 20.0%
!! ATTTACTTTGATATCTTTAG+AGG - chr2_4:63805737-63805756 Msa0341860:intron 20.0%
!! TATATTCAAATTGTTTGCAA+TGG - chr2_4:63806033-63806052 Msa0341860:CDS 20.0%
!!! AAATGGAATGATTTTGTAAA+TGG - chr2_4:63806089-63806108 Msa0341860:five_prime_UTR 20.0%
! TTAACAGAGAGATAAAAGAA+CGG - chr2_4:63801580-63801599 Msa0341860:exon 25.0%
! TATTAGAAAATGGATGTTCA+TGG - chr2_4:63802040-63802059 Msa0341860:intron 25.0%
! ATTAGAAAATGGATGTTCAT+GGG - chr2_4:63802041-63802060 Msa0341860:intron 25.0%
!!! TTTTAAATGGTTGGTATAGA+AGG + chr2_4:63802214-63802233 None:intergenic 25.0%
! TAAATAAAAAAAGTGGCCAA+AGG - chr2_4:63802238-63802257 Msa0341860:intron 25.0%
! AAATAAAAAAAGTGGCCAAA+GGG - chr2_4:63802239-63802258 Msa0341860:intron 25.0%
! TAAAGAAGAATAGAAACTTG+CGG + chr2_4:63802289-63802308 None:intergenic 25.0%
!! ATCAAACCATTTTCATAAGT+TGG - chr2_4:63802487-63802506 Msa0341860:intron 25.0%
!!! TATCGCGATACAATTTTTTT+AGG - chr2_4:63802620-63802639 Msa0341860:CDS 25.0%
!!! ATTCCTAGATGAACATTTTT+TGG - chr2_4:63802995-63803014 Msa0341860:intron 25.0%
!!! ATTTTTTTGCGTTAACACTT+CGG + chr2_4:63803081-63803100 None:intergenic 25.0%
!! TGGTGATTCTAATTTTATCA+TGG - chr2_4:63803204-63803223 Msa0341860:intron 25.0%
! ACTAAAAGGTAAATAGAGAT+TGG - chr2_4:63803704-63803723 Msa0341860:intron 25.0%
! TTATATAAGCTCATCAATAG+AGG - chr2_4:63804044-63804063 Msa0341860:CDS 25.0%
! TTTAGGTGTCTAAATTGATT+CGG - chr2_4:63804292-63804311 Msa0341860:intron 25.0%
! TCAATGCAATCATATTACTT+TGG - chr2_4:63804454-63804473 Msa0341860:intron 25.0%
!! TTTGTTGTCATTCATGTTAT+AGG - chr2_4:63804970-63804989 Msa0341860:intron 25.0%
!! AGCTAAACAAATAGCAATTT+TGG + chr2_4:63805394-63805413 None:intergenic 25.0%
! TTTCAGATTTGCATTATACT+TGG - chr2_4:63805801-63805820 Msa0341860:CDS 25.0%
! TAAAATGCAAGATATGACAA+AGG + chr2_4:63805954-63805973 None:intergenic 25.0%
!! TTTTGTAAATGGAGTGATTT+AGG - chr2_4:63806100-63806119 Msa0341860:five_prime_UTR 25.0%
!!! GCAATTGGTTTTTCATTTGT+TGG + chr2_4:63801433-63801452 None:intergenic 30.0%
TATGTTGTTAAGTTTACCTC+TGG + chr2_4:63801496-63801515 None:intergenic 30.0%
! AACAAAAGGCAATTGATGAT+TGG - chr2_4:63801606-63801625 Msa0341860:CDS 30.0%
!! GAACTTCTAGTTCTAGAATA+CGG + chr2_4:63801792-63801811 None:intergenic 30.0%
!! ATTCTAGAACTAGAAGTTCA+TGG - chr2_4:63801793-63801812 Msa0341860:CDS 30.0%
CTCATATTCTCATACTTGAT+AGG + chr2_4:63801829-63801848 None:intergenic 30.0%
!! ATTTTTCAAACAAGGGTTGA+CGG + chr2_4:63801867-63801886 None:intergenic 30.0%
AATATGAGTGACCATATGAT+TGG - chr2_4:63801896-63801915 Msa0341860:intron 30.0%
TCAAACTTGTACCAATCATA+TGG + chr2_4:63801910-63801929 None:intergenic 30.0%
GGGATTTGACATGTAATTTA+CGG + chr2_4:63802316-63802335 None:intergenic 30.0%
TAGCTACCAACTTATGAAAA+TGG + chr2_4:63802496-63802515 None:intergenic 30.0%
TATTACCTCTCGAACAAATT+GGG - chr2_4:63802916-63802935 Msa0341860:intron 30.0%
TGACCAAAAAATGTTCATCT+AGG + chr2_4:63803001-63803020 None:intergenic 30.0%
! TTTTACAATGTCGAATTGCA+GGG - chr2_4:63803172-63803191 Msa0341860:intron 30.0%
ACTGCAGAAAATTCATGATT+TGG - chr2_4:63803418-63803437 Msa0341860:intron 30.0%
AAACGACCAAATTGAAGAAT+TGG + chr2_4:63803750-63803769 None:intergenic 30.0%
TCTTCAATTTGGTCGTTTAA+TGG - chr2_4:63803752-63803771 Msa0341860:intron 30.0%
!! CAATTTTGAAGATGATGTGT+TGG - chr2_4:63803832-63803851 Msa0341860:intron 30.0%
!! AAGTCTTTCTAAAGTCTCTA+TGG + chr2_4:63803963-63803982 None:intergenic 30.0%
! ATAGAGACTTTAGAAAGACT+TGG - chr2_4:63803962-63803981 Msa0341860:CDS 30.0%
! GATTGGATTGTTCTTTTAGT+CGG + chr2_4:63804088-63804107 None:intergenic 30.0%
TCAAATTGGTCACAATATGT+GGG + chr2_4:63804269-63804288 None:intergenic 30.0%
! ATTTCTGGACTAAAGTCAAA+TGG + chr2_4:63804387-63804406 None:intergenic 30.0%
TGCAATCATATTACTTTGGT+AGG - chr2_4:63804458-63804477 Msa0341860:intron 30.0%
GCAATCATATTACTTTGGTA+GGG - chr2_4:63804459-63804478 Msa0341860:intron 30.0%
TAGTTACTCAACAATTGCAT+GGG - chr2_4:63804606-63804625 Msa0341860:intron 30.0%
AAGAAATTGAAAACAGTTCC+TGG + chr2_4:63804694-63804713 None:intergenic 30.0%
TTCAATTTCTTCAGTGCTTT+AGG - chr2_4:63804703-63804722 Msa0341860:intron 30.0%
CTTCTCTTAATAAACCTACT+TGG - chr2_4:63804926-63804945 Msa0341860:intron 30.0%
TGTGAAGAATCATATGCAAA+TGG + chr2_4:63805030-63805049 None:intergenic 30.0%
!!! ACGCAATTTTTTGACCATTA+CGG + chr2_4:63805136-63805155 None:intergenic 30.0%
! ATTTTATCGTTATCCGATGA+TGG - chr2_4:63805323-63805342 Msa0341860:intron 30.0%
GGACTTACAAAATATGTTGT+GGG + chr2_4:63805522-63805541 None:intergenic 30.0%
TGGACTTACAAAATATGTTG+TGG + chr2_4:63805523-63805542 None:intergenic 30.0%
!! TTGCTATTTACAAACCTAAG+AGG - chr2_4:63805656-63805675 Msa0341860:intron 30.0%
TTTCTATACTTGACGATTCA+TGG - chr2_4:63805893-63805912 Msa0341860:intron 30.0%
ATTCAAATTGTTTGCAATGG+AGG - chr2_4:63806036-63806055 Msa0341860:CDS 30.0%
TTCAAATTGTTTGCAATGGA+GGG - chr2_4:63806037-63806056 Msa0341860:CDS 30.0%
!!! ATTTTAGCTCTCTCAGAAAA+TGG - chr2_4:63806072-63806091 Msa0341860:exon 30.0%
ACTTGACTTAATAACCACCA+TGG - chr2_4:63801396-63801415 Msa0341860:three_prime_UTR 35.0%
CTTGACTTAATAACCACCAT+GGG - chr2_4:63801397-63801416 Msa0341860:three_prime_UTR 35.0%
AAAACCAATTGCTTCTTCAG+AGG - chr2_4:63801441-63801460 Msa0341860:three_prime_UTR 35.0%
TAAAAGAACGGCTGAACAAA+AGG - chr2_4:63801592-63801611 Msa0341860:CDS 35.0%
GCATTCCTTGAAGAAGTTAT+TGG + chr2_4:63801635-63801654 None:intergenic 35.0%
CTTCTTCAAGGAATGCAAAA+TGG - chr2_4:63801639-63801658 Msa0341860:CDS 35.0%
TCATACTTGATAGGACGAAA+AGG + chr2_4:63801820-63801839 None:intergenic 35.0%
AACAATAACCTAAACGGTGT+TGG + chr2_4:63802007-63802026 None:intergenic 35.0%
TGAAAATCATATAACCTCCC+CGG - chr2_4:63802854-63802873 Msa0341860:CDS 35.0%
CTATTACCTCTCGAACAAAT+TGG - chr2_4:63802915-63802934 Msa0341860:intron 35.0%
TCATCAATGACGAATTGTTC+AGG - chr2_4:63802958-63802977 Msa0341860:intron 35.0%
! GTTAACACTTCGGTTCTAAT+GGG + chr2_4:63803071-63803090 None:intergenic 35.0%
ACCACTAATTTGACATCTGA+AGG - chr2_4:63803113-63803132 Msa0341860:intron 35.0%
TCCTTCAGATGTCAAATTAG+TGG + chr2_4:63803117-63803136 None:intergenic 35.0%
! GTTTTACAATGTCGAATTGC+AGG - chr2_4:63803171-63803190 Msa0341860:intron 35.0%
TTTACAATGTCGAATTGCAG+GGG - chr2_4:63803173-63803192 Msa0341860:intron 35.0%
AAAATTAGAATCACCACACC+AGG + chr2_4:63803200-63803219 None:intergenic 35.0%
AATGGTTGAGATGCATGAAA+TGG - chr2_4:63803253-63803272 Msa0341860:intron 35.0%
ATGAAATGGTTCCAGGAAAA+CGG - chr2_4:63803267-63803286 Msa0341860:intron 35.0%
TTTGACAGATACCATGAGTT+GGG - chr2_4:63803290-63803309 Msa0341860:intron 35.0%
ACTTCACATATCAACTGTTG+TGG + chr2_4:63803359-63803378 None:intergenic 35.0%
! CAACAGTTGATATGTGAAGT+TGG - chr2_4:63803359-63803378 Msa0341860:intron 35.0%
GGCAAGAATAAAATGAACAG+AGG + chr2_4:63803498-63803517 None:intergenic 35.0%
!! GGTTGATGATTGATACTGAA+TGG + chr2_4:63803605-63803624 None:intergenic 35.0%
TAGCGACCAATTCTTCAATT+TGG - chr2_4:63803741-63803760 Msa0341860:intron 35.0%
AGTTATCAAGTACCTTACCT+TGG - chr2_4:63803924-63803943 Msa0341860:CDS 35.0%
GTCAAATTGGTCACAATATG+TGG + chr2_4:63804270-63804289 None:intergenic 35.0%
! TGTGACCAATTTGACGTTTT+AGG - chr2_4:63804275-63804294 Msa0341860:CDS 35.0%
AGACACCTAAAACGTCAAAT+TGG + chr2_4:63804283-63804302 None:intergenic 35.0%
TAAGATCAGGGTTCAAACAT+CGG - chr2_4:63804499-63804518 Msa0341860:intron 35.0%
GTAGTTACTCAACAATTGCA+TGG - chr2_4:63804605-63804624 Msa0341860:intron 35.0%
ATGGGTGGCTTCATTAAAAA+AGG - chr2_4:63804624-63804643 Msa0341860:intron 35.0%
TGGGTGGCTTCATTAAAAAA+GGG - chr2_4:63804625-63804644 Msa0341860:intron 35.0%
AATAAAACTCCTCTCCACAT+TGG + chr2_4:63804817-63804836 None:intergenic 35.0%
TATTTCCCTGTTGCTCTTAT+TGG - chr2_4:63804862-63804881 Msa0341860:intron 35.0%
ATTTCCCTGTTGCTCTTATT+GGG - chr2_4:63804863-63804882 Msa0341860:intron 35.0%
AATACCCAATAAGAGCAACA+GGG + chr2_4:63804870-63804889 None:intergenic 35.0%
!! CTTATTGGGTATTGGATGTT+TGG - chr2_4:63804877-63804896 Msa0341860:intron 35.0%
TCATGTTATAGGAAGCTATC+AGG - chr2_4:63804981-63805000 Msa0341860:intron 35.0%
AAGCTATCAGGTATGTATGA+TGG - chr2_4:63804993-63805012 Msa0341860:intron 35.0%
GTCTCAATCATGTCAAAAAC+AGG + chr2_4:63805114-63805133 None:intergenic 35.0%
AACAACAAATCTTAGGAGTC+GGG + chr2_4:63805169-63805188 None:intergenic 35.0%
GAACAACAAATCTTAGGAGT+CGG + chr2_4:63805170-63805189 None:intergenic 35.0%
CATTGCGAACAACAAATCTT+AGG + chr2_4:63805176-63805195 None:intergenic 35.0%
GTTGTTCGCAATGTTTATGT+TGG - chr2_4:63805183-63805202 Msa0341860:intron 35.0%
TTCAGCATTCACAATGTTTG+TGG - chr2_4:63805437-63805456 Msa0341860:intron 35.0%
TCAGCATTCACAATGTTTGT+GGG - chr2_4:63805438-63805457 Msa0341860:intron 35.0%
! CTATTGGTGACAGAATCATA+AGG + chr2_4:63805833-63805852 None:intergenic 35.0%
CTTATGATTCTGTCACCAAT+AGG - chr2_4:63805831-63805850 Msa0341860:CDS 35.0%
TCATGGATGCATGTGTATAA+TGG - chr2_4:63805910-63805929 Msa0341860:intron 35.0%
!!! TTTTGTAAGGGTGCTTTGAT+TGG - chr2_4:63805987-63806006 Msa0341860:intron 35.0%
GATTGGAACAAATGGAGATA+AGG - chr2_4:63806004-63806023 Msa0341860:CDS 35.0%
TCAAATTGTTTGCAATGGAG+GGG - chr2_4:63806038-63806057 Msa0341860:CDS 35.0%
GGCTTCCAATAACTTCTTCA+AGG - chr2_4:63801627-63801646 Msa0341860:CDS 40.0%
CTTCAAGGAATGCAAAATGG+TGG - chr2_4:63801642-63801661 Msa0341860:CDS 40.0%
! TTTTCACAACGTTACTGCCA+TGG - chr2_4:63801673-63801692 Msa0341860:intron 40.0%
TTGACAAACCAACACCGTTT+AGG - chr2_4:63801996-63802015 Msa0341860:CDS 40.0%
AAGAAGACAACGATCTTCGT+TGG - chr2_4:63802111-63802130 Msa0341860:intron 40.0%
GAAAATCATATAACCTCCCC+GGG - chr2_4:63802855-63802874 Msa0341860:CDS 40.0%
AAAATCATATAACCTCCCCG+GGG - chr2_4:63802856-63802875 Msa0341860:CDS 40.0%
AGACGAAATTTAAATCCCCC+GGG + chr2_4:63802875-63802894 None:intergenic 40.0%
GAGACGAAATTTAAATCCCC+CGG + chr2_4:63802876-63802895 None:intergenic 40.0%
GTTTGGTCATAAGGGAAGTA+AGG + chr2_4:63803026-63803045 None:intergenic 40.0%
AATCCGGAGTTTGGTCATAA+GGG + chr2_4:63803034-63803053 None:intergenic 40.0%
TAATCCGGAGTTTGGTCATA+AGG + chr2_4:63803035-63803054 None:intergenic 40.0%
GGAAAGAAGTCCTGATAATC+CGG + chr2_4:63803050-63803069 None:intergenic 40.0%
! CGTTAACACTTCGGTTCTAA+TGG + chr2_4:63803072-63803091 None:intergenic 40.0%
TAATCACCCTCTACACTCTA+TGG - chr2_4:63803228-63803247 Msa0341860:intron 40.0%
CATTTGCCATAGAGTGTAGA+GGG + chr2_4:63803237-63803256 None:intergenic 40.0%
! TATCTGTCAAACCGTTTTCC+TGG + chr2_4:63803281-63803300 None:intergenic 40.0%
GTTTGACAGATACCATGAGT+TGG - chr2_4:63803289-63803308 Msa0341860:intron 40.0%
! GAAGCATTTGGAGAAAAGCT+TGG - chr2_4:63803317-63803336 Msa0341860:intron 40.0%
!! GTTGATATGTGAAGTTGGTG+TGG - chr2_4:63803364-63803383 Msa0341860:intron 40.0%
TGGTGTGGACATTGTTTACA+TGG - chr2_4:63803379-63803398 Msa0341860:intron 40.0%
GACATTGTTTACATGGTCAC+CGG - chr2_4:63803386-63803405 Msa0341860:intron 40.0%
ACATTGTTTACATGGTCACC+GGG - chr2_4:63803387-63803406 Msa0341860:intron 40.0%
TTATGAGAGAGCCAAACTCA+TGG - chr2_4:63803858-63803877 Msa0341860:intron 40.0%
ACCATTGGTATCCATGAGTT+TGG + chr2_4:63803872-63803891 None:intergenic 40.0%
TTATTGAGAATGGGTCGTGA+TGG + chr2_4:63804110-63804129 None:intergenic 40.0%
TTACTCAACAATTGCATGGG+TGG - chr2_4:63804609-63804628 Msa0341860:intron 40.0%
CAATACCCAATAAGAGCAAC+AGG + chr2_4:63804871-63804890 None:intergenic 40.0%
CTGTTGCTCTTATTGGGTAT+TGG - chr2_4:63804869-63804888 Msa0341860:intron 40.0%
TGACATGATTGAGACCGTAA+TGG - chr2_4:63805119-63805138 Msa0341860:intron 40.0%
!! GGTGAGCACCTTTCTAAAAT+TGG - chr2_4:63805204-63805223 Msa0341860:intron 40.0%
!! ATCCGATGATGGAGATTTCA+TGG - chr2_4:63805334-63805353 Msa0341860:intron 40.0%
!! TCCGATGATGGAGATTTCAT+GGG - chr2_4:63805335-63805354 Msa0341860:intron 40.0%
ACCCATGAAATCTCCATCAT+CGG + chr2_4:63805339-63805358 None:intergenic 40.0%
GGAATAACCTTCCCATTCTT+TGG - chr2_4:63805459-63805478 Msa0341860:intron 40.0%
TCCTCCAAAAAATCCAAGGA+GGG + chr2_4:63805487-63805506 None:intergenic 40.0%
ATCCTCCAAAAAATCCAAGG+AGG + chr2_4:63805488-63805507 None:intergenic 40.0%
CCAATCCTCCAAAAAATCCA+AGG + chr2_4:63805491-63805510 None:intergenic 40.0%
!!! CCTTGGATTTTTTGGAGGAT+TGG - chr2_4:63805488-63805507 Msa0341860:intron 40.0%
TGTAGCTTCCTTGCATTATG+TGG - chr2_4:63805632-63805651 Msa0341860:intron 40.0%
ATAGCAAGCCACATAATGCA+AGG + chr2_4:63805643-63805662 None:intergenic 40.0%
CAAGACAAGCTAAACCTCTT+AGG + chr2_4:63805673-63805692 None:intergenic 40.0%
!! CTAAGAGGTTTAGCTTGTCT+TGG - chr2_4:63805671-63805690 Msa0341860:intron 40.0%
CTTGTCTTGGTTCACAAACT+GGG - chr2_4:63805684-63805703 Msa0341860:intron 40.0%
! TTGGTGACAGAATCATAAGG+AGG + chr2_4:63805830-63805849 None:intergenic 40.0%
ATGATTCTGTCACCAATAGG+TGG - chr2_4:63805834-63805853 Msa0341860:CDS 40.0%
! ATTAGCCTCAATCCACCTAT+TGG + chr2_4:63805849-63805868 None:intergenic 40.0%
ATACTCAATGCCAAGTCCTA+CGG - chr2_4:63805870-63805889 Msa0341860:CDS 40.0%
AGTATAGAAACCGTAGGACT+TGG + chr2_4:63805883-63805902 None:intergenic 40.0%
TCGTCAAGTATAGAAACCGT+AGG + chr2_4:63805889-63805908 None:intergenic 40.0%
ATGCAAGATATGACAAAGGC+AGG + chr2_4:63805950-63805969 None:intergenic 40.0%
GGTGCTTTGATTGGAACAAA+TGG - chr2_4:63805996-63806015 Msa0341860:intron 40.0%
! TCAGCCTCTGAAGAAGCAAT+TGG + chr2_4:63801448-63801467 None:intergenic 45.0%
TGCTTCTTCAGAGGCTGAAA+AGG - chr2_4:63801450-63801469 Msa0341860:three_prime_UTR 45.0%
TTTCTCAGCACAAGAACCAG+AGG - chr2_4:63801477-63801496 Msa0341860:three_prime_UTR 45.0%
ATACCATCCAAGACTTGCCA+TGG + chr2_4:63801729-63801748 None:intergenic 45.0%
AAATCATATAACCTCCCCGG+GGG - chr2_4:63802857-63802876 Msa0341860:CDS 45.0%
GACGAAATTTAAATCCCCCG+GGG + chr2_4:63802874-63802893 None:intergenic 45.0%
CTTCCCTTATGACCAAACTC+CGG - chr2_4:63803028-63803047 Msa0341860:intron 45.0%
TGACCAAACTCCGGATTATC+AGG - chr2_4:63803037-63803056 Msa0341860:intron 45.0%
AGTCCTGATAATCCGGAGTT+TGG + chr2_4:63803043-63803062 None:intergenic 45.0%
CCATTTGCCATAGAGTGTAG+AGG + chr2_4:63803238-63803257 None:intergenic 45.0%
CCTCTACACTCTATGGCAAA+TGG - chr2_4:63803235-63803254 Msa0341860:intron 45.0%
GAGATGCATGAAATGGTTCC+AGG - chr2_4:63803260-63803279 Msa0341860:intron 45.0%
ATGCTTCTTGTCCCAACTCA+TGG + chr2_4:63803304-63803323 None:intergenic 45.0%
!! GAGTTGGGACAAGAAGCATT+TGG - chr2_4:63803305-63803324 Msa0341860:intron 45.0%
TTGGAGAAAAGCTTGGACTC+TGG - chr2_4:63803324-63803343 Msa0341860:intron 45.0%
CATTGTTTACATGGTCACCG+GGG - chr2_4:63803388-63803407 Msa0341860:intron 45.0%
ATTGTTTACATGGTCACCGG+GGG - chr2_4:63803389-63803408 Msa0341860:intron 45.0%
! TTTCTGCAGTGATTTTCCCC+CGG + chr2_4:63803408-63803427 None:intergenic 45.0%
GGAGTTGAAGTTGGGAAGAT+GGG + chr2_4:63803519-63803538 None:intergenic 45.0%
TGGAGTTGAAGTTGGGAAGA+TGG + chr2_4:63803520-63803539 None:intergenic 45.0%
CCAGCAATGGAGTTGAAGTT+GGG + chr2_4:63803527-63803546 None:intergenic 45.0%
ACCAGCAATGGAGTTGAAGT+TGG + chr2_4:63803528-63803547 None:intergenic 45.0%
CTGCAGCAATCATGTCTTTG+AGG - chr2_4:63803558-63803577 Msa0341860:intron 45.0%
GCCAAACTCATGGATACCAA+TGG - chr2_4:63803868-63803887 Msa0341860:intron 45.0%
ACCTTACCTTGGATGATCGA+AGG - chr2_4:63803935-63803954 Msa0341860:CDS 45.0%
TCCTTCGATCATCCAAGGTA+AGG + chr2_4:63803939-63803958 None:intergenic 45.0%
ATGGATCCTTCGATCATCCA+AGG + chr2_4:63803944-63803963 None:intergenic 45.0%
ACACGTTGCTCATCAGTAAG+AGG + chr2_4:63804007-63804026 None:intergenic 45.0%
AAAGGGAGTTCAACCAGATG+TGG - chr2_4:63804642-63804661 Msa0341860:intron 45.0%
!! AGTTGTGGCTTTGTAGCCAT+AGG + chr2_4:63804668-63804687 None:intergenic 45.0%
TACAAAGCCACAACTACACC+AGG - chr2_4:63804673-63804692 Msa0341860:intron 45.0%
AACAGTTCCTGGTGTAGTTG+TGG + chr2_4:63804683-63804702 None:intergenic 45.0%
GATGTTGCATTTGCCTATGC+CGG - chr2_4:63804727-63804746 Msa0341860:intron 45.0%
GCTGCAACTATAAGCCAAGT+AGG + chr2_4:63804943-63804962 None:intergenic 45.0%
ACAAATCTTAGGAGTCGGGT+TGG + chr2_4:63805165-63805184 None:intergenic 45.0%
GCTTGTCTTGGTTCACAAAC+TGG - chr2_4:63805683-63805702 Msa0341860:intron 45.0%
GTGACAGAATCATAAGGAGG+AGG + chr2_4:63805827-63805846 None:intergenic 45.0%
!! TGTCACCAATAGGTGGATTG+AGG - chr2_4:63805841-63805860 Msa0341860:CDS 45.0%
AATTGTTTGCAATGGAGGGG+AGG - chr2_4:63806041-63806060 Msa0341860:CDS 45.0%
ATTGTTTGCAATGGAGGGGA+GGG - chr2_4:63806042-63806061 Msa0341860:CDS 45.0%
!! AAAAATATAAATAAAAAAAG+TGG - chr2_4:63802231-63802250 Msa0341860:intron 5.0%
!!! ATTTTTTTAAAAAAAAACTA+AGG - chr2_4:63802792-63802811 Msa0341860:CDS 5.0%
!!! TTATTTTATATATTAATTTC+TGG + chr2_4:63804402-63804421 None:intergenic 5.0%
!!! TTAAAGAAAAATAAATTTTA+TGG + chr2_4:63805543-63805562 None:intergenic 5.0%
!!! TATTTTTCTTTAAATATATT+TGG - chr2_4:63805550-63805569 Msa0341860:intron 5.0%
TGTTGGAGACTCAGTTCCCA+TGG + chr2_4:63801416-63801435 None:intergenic 50.0%
CACAACGTTACTGCCATGGT+TGG - chr2_4:63801677-63801696 Msa0341860:intron 50.0%
CTTGAGTCTTCCATCTGCCA+TGG - chr2_4:63801709-63801728 Msa0341860:intron 50.0%
GAAATTTAAATCCCCCGGGG+AGG + chr2_4:63802871-63802890 None:intergenic 50.0%
! CTGAGCCCAATTTGTTCGAG+AGG + chr2_4:63802924-63802943 None:intergenic 50.0%
CTCTCGAACAAATTGGGCTC+AGG - chr2_4:63802922-63802941 Msa0341860:intron 50.0%
TCGAACAAATTGGGCTCAGG+TGG - chr2_4:63802925-63802944 Msa0341860:intron 50.0%
CCCAACTTCAACTCCATTGC+TGG - chr2_4:63803524-63803543 Msa0341860:intron 50.0%
CTCCATTGCTGGTATCTCCT+TGG - chr2_4:63803535-63803554 Msa0341860:intron 50.0%
AGCCAAGGAGATACCAGCAA+TGG + chr2_4:63803540-63803559 None:intergenic 50.0%
AGACATGATTGCTGCAGCCA+AGG + chr2_4:63803555-63803574 None:intergenic 50.0%
!! GAGAATGGGTCGTGATGGAT+TGG + chr2_4:63804105-63804124 None:intergenic 50.0%
GTTCAACCAGATGTGGCCTA+TGG - chr2_4:63804649-63804668 Msa0341860:intron 50.0%
TTGTAGCCATAGGCCACATC+TGG + chr2_4:63804658-63804677 None:intergenic 50.0%
CTCCAACACCACATTGTGTC+CGG + chr2_4:63804749-63804768 None:intergenic 50.0%
ACACCGGAGAAACCATCCAA+GGG - chr2_4:63804790-63804809 Msa0341860:intron 50.0%
AACCATCCAAGGGTCCAATG+TGG - chr2_4:63804800-63804819 Msa0341860:intron 50.0%
!! CAAGGAGGGCACCAAAGAAT+GGG + chr2_4:63805473-63805492 None:intergenic 50.0%
!!! GGTGCCCTCCTTGGATTTTT+TGG - chr2_4:63805480-63805499 Msa0341860:intron 50.0%
!!! GCCCTCCTTGGATTTTTTGG+AGG - chr2_4:63805483-63805502 Msa0341860:intron 50.0%
TGGAGACTCAGTTCCCATGG+TGG + chr2_4:63801413-63801432 None:intergenic 55.0%
GTTACTGCCATGGTTGGAGC+TGG - chr2_4:63801683-63801702 Msa0341860:intron 55.0%
CAAGACACCAGCTCCAACCA+TGG + chr2_4:63801693-63801712 None:intergenic 55.0%
CCAAGACTTGCCATGGCAGA+TGG + chr2_4:63801722-63801741 None:intergenic 55.0%
CCATCTGCCATGGCAAGTCT+TGG - chr2_4:63801719-63801738 Msa0341860:intron 55.0%
CTGCCATGGCAAGTCTTGGA+TGG - chr2_4:63801723-63801742 Msa0341860:intron 55.0%
ATGTCGAATTGCAGGGGTCC+TGG - chr2_4:63803179-63803198 Msa0341860:intron 55.0%
TGCCTATGCCGGACACAATG+TGG - chr2_4:63804738-63804757 Msa0341860:intron 55.0%
CACCACATTGTGTCCGGCAT+AGG + chr2_4:63804743-63804762 None:intergenic 55.0%
TGCCGGACACAATGTGGTGT+TGG - chr2_4:63804744-63804763 Msa0341860:intron 55.0%
AGCAACAATCCCGTCCACAC+CGG - chr2_4:63804774-63804793 Msa0341860:intron 55.0%
ATGGTTTCTCCGGTGTGGAC+GGG + chr2_4:63804786-63804805 None:intergenic 55.0%
GATGGTTTCTCCGGTGTGGA+CGG + chr2_4:63804787-63804806 None:intergenic 55.0%
CTTGGATGGTTTCTCCGGTG+TGG + chr2_4:63804791-63804810 None:intergenic 55.0%
CACACCGGAGAAACCATCCA+AGG - chr2_4:63804789-63804808 Msa0341860:intron 55.0%
GGACCCTTGGATGGTTTCTC+CGG + chr2_4:63804796-63804815 None:intergenic 55.0%
CTCCACATTGGACCCTTGGA+TGG + chr2_4:63804805-63804824 None:intergenic 55.0%
TCCAAGGGTCCAATGTGGAG+AGG - chr2_4:63804805-63804824 Msa0341860:intron 55.0%
TCCTCTCCACATTGGACCCT+TGG + chr2_4:63804809-63804828 None:intergenic 55.0%
!! GAGGGCACCAAAGAATGGGA+AGG + chr2_4:63805469-63805488 None:intergenic 55.0%
!! CCAAGGAGGGCACCAAAGAA+TGG + chr2_4:63805474-63805493 None:intergenic 55.0%
!! CCATTCTTTGGTGCCCTCCT+TGG - chr2_4:63805471-63805490 Msa0341860:intron 55.0%
GAATTGCAGGGGTCCTGGTG+TGG - chr2_4:63803184-63803203 Msa0341860:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 63801273 63806223 63801273 ID=Msa0341860;Name=Msa0341860
chr2_4 mRNA 63801273 63806223 63801273 ID=Msa0341860-mRNA-1;Parent=Msa0341860;Name=Msa0341860-mRNA-1;_AED=0.09;_eAED=0.09;_QI=142|1|1|1|1|1|8|318|462
chr2_4 exon 63801273 63801689 63801273 ID=Msa0341860-mRNA-1:exon:20465;Parent=Msa0341860-mRNA-1
chr2_4 exon 63801791 63801859 63801791 ID=Msa0341860-mRNA-1:exon:20464;Parent=Msa0341860-mRNA-1
chr2_4 exon 63801963 63802054 63801963 ID=Msa0341860-mRNA-1:exon:20463;Parent=Msa0341860-mRNA-1
chr2_4 exon 63802292 63802397 63802292 ID=Msa0341860-mRNA-1:exon:20462;Parent=Msa0341860-mRNA-1
chr2_4 exon 63802494 63802887 63802494 ID=Msa0341860-mRNA-1:exon:20461;Parent=Msa0341860-mRNA-1
chr2_4 exon 63803917 63804303 63803917 ID=Msa0341860-mRNA-1:exon:20460;Parent=Msa0341860-mRNA-1
chr2_4 exon 63805752 63805909 63805752 ID=Msa0341860-mRNA-1:exon:20459;Parent=Msa0341860-mRNA-1
chr2_4 exon 63805998 63806223 63805998 ID=Msa0341860-mRNA-1:exon:20458;Parent=Msa0341860-mRNA-1
chr2_4 five_prime_UTR 63806082 63806223 63806082 ID=Msa0341860-mRNA-1:five_prime_utr;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63805998 63806081 63805998 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63805752 63805909 63805752 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63803917 63804303 63803917 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63802494 63802887 63802494 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63802292 63802397 63802292 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63801963 63802054 63801963 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63801791 63801859 63801791 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 CDS 63801591 63801689 63801591 ID=Msa0341860-mRNA-1:cds;Parent=Msa0341860-mRNA-1
chr2_4 three_prime_UTR 63801273 63801590 63801273 ID=Msa0341860-mRNA-1:three_prime_utr;Parent=Msa0341860-mRNA-1
Gene Sequence

>Msa0341860

ATGGGAACTGAGTCTCCAACAAATGAAAAACCAATTGCTTCTTCAGAGGCTGAAAAGGTCTCTTTCTCAGCACAAGAACCAGAGAGAGATAAAAGAACGGCTGAACAAAAGGCAATTGATGATTGGCTTCCAATAACTTCTTCAAGGAATGCAAAATGGTGGTACGCAGCTTTTCACAACGTTACTGCCATGGTTGGAGCTGGTGTCTTGAGTCTTCCATCTGCCATGGCAAGTCTTGGATGGGGTCCTGGTGTGGTGATTCTAATTTTATCATGGATAATCACCCTCTACACTCTATGGCAAATGGTTGAGATGCATGAAATGGTTCCAGGAAAACGGTTTGACAGATACCATGAGTTGGGACAAGAAGCATTTGGAGAAAAGCTTGGACTCTGGATTGTCGTGCCACAACAGTTGATATGTGAAGTTGGTGTGGACATTGTTTACATGGTCACCGGGGGAAAATCACTGCAGAAAATTCATGATTTGGTGTGTAAAAAAAATTGCAAATCAATGAAAACTACTTATTTCATCATGATATTTGCCTCTGTTCATTTTATTCTTGCCCATCTTCCCAACTTCAACTCCATTGCTGGTATCTCCTTGGCTGCAGCAATCATGTCTTTGAGTTACTCAACAATTGCATGGGTGGCTTCATTAAAAAAGGGAGTTCAACCAGATGTGGCCTATGGCTACAAAGCCACAACTACACCAGGAACTGTTTTCAATTTCTTCAGTGCTTTAGGTGATGTTGCATTTGCCTATGCCGGACACAATGTGGTGTTGGAGATTCAAGCAACAATCCCGTCCACACCGGAGAAACCATCCAAGGGTCCAATGTGGAGAGGAGTTTTATTAGCATATATTGTTGTAGCTTTGTGTTATTTCCCTGTTGCTCTTATTGGGTATTGGATGTTTGGCAATTCAGTTGCTGATAATATTCTTACTTCTCTTAATAAACCTACTTGGCTTATAGTTGCAGCTAACATGTTTGTTGTCATTCATGTTATAGGAAGCTATCAGTTGTATGCAATGCCTGTTTTTGACATGATTGAGACCGTAATGGTCAAAAAATTGCGTTTCAAGCCAACCCGACTCCTAAGATTTGTTGTTCGCAATGTTTATGTTGCATTCACAATGTTTGTGGGAATAACCTTCCCATTCTTTGGTGCCCTCCTTGGATTTTTTGGAGGATTGGCATTTGCTCCCACAACATATTTTCTTCCTTGCATTATGTGGCTTGCTATTTACAAACCTAAGAGGTTTAGCTTGTCTTGGTTCACAAACTGGATTTGCATTATACTTGGCCTCCTCCTTATGATTCTGTCACCAATAGGTGGATTGAGGCTAATCATACTCAATGCCAAGTCCTACGGTTTCTATACTTGA
Protein sequence

>Msa0341860

MGTESPTNEKPIASSEAEKVSFSAQEPERDKRTAEQKAIDDWLPITSSRNAKWWYAAFHNVTAMVGAGVLSLPSAMASLGWGPGVVILILSWIITLYTLWQMVEMHEMVPGKRFDRYHELGQEAFGEKLGLWIVVPQQLICEVGVDIVYMVTGGKSLQKIHDLVCKKNCKSMKTTYFIMIFASVHFILAHLPNFNSIAGISLAAAIMSLSYSTIAWVASLKKGVQPDVAYGYKATTTPGTVFNFFSALGDVAFAYAGHNVVLEIQATIPSTPEKPSKGPMWRGVLLAYIVVALCYFPVALIGYWMFGNSVADNILTSLNKPTWLIVAANMFVVIHVIGSYQLYAMPVFDMIETVMVKKLRFKPTRLLRFVVRNVYVAFTMFVGITFPFFGALLGFFGGLAFAPTTYFLPCIMWLAIYKPKRFSLSWFTNWICIILGLLLMILSPIGGLRLIILNAKSYGFYT*