AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0930550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0930550 MtrunA17_Chr8g0392091 100.000 108 0 0 1 108 1 108 1.74e-76 221
Msa0930550 MtrunA17_Chr5g0447361 93.137 102 7 0 7 108 2 103 6.28e-66 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0930550 AT2G47640.3 93.519 108 7 0 1 108 1 108 3.58e-71 207
Msa0930550 AT2G47640.2 93.519 108 7 0 1 108 1 108 3.58e-71 207
Msa0930550 AT3G62840.2 93.519 108 7 0 1 108 1 108 3.58e-71 207
Msa0930550 AT2G47640.1 92.661 109 7 1 1 108 1 109 1.41e-69 203
Msa0930550 AT2G47640.4 92.661 109 7 1 1 108 1 109 1.41e-69 203
Msa0930550 AT3G62840.1 92.661 109 7 1 1 108 1 109 1.41e-69 203

Find 65 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGGAGATACTAGCTTATT+TGG 0.120204 6_2:-79651937 None:intergenic
CGCATTATTGGTATGAAATA+TGG 0.156755 6_2:+79652003 Msa0930550:three_prime_UTR
CTCCACGTTTCAATAAAATT+TGG 0.187552 6_2:-79652039 None:intergenic
TCAACTTCATTAGAGGAATT+TGG 0.199510 6_2:+79650609 Msa0930550:five_prime_UTR
AGAGAATACATACCTCATTT+TGG 0.226756 6_2:-79650654 None:intergenic
TACTAAACTATTTATACTTT+TGG 0.249365 6_2:-79652258 None:intergenic
TGCAGTTTCAACTTCATTAG+AGG 0.275858 6_2:+79650602 Msa0930550:intron
TTATTGAAACGTGGAGAAAT+TGG 0.278647 6_2:+79652046 Msa0930550:three_prime_UTR
ATTCTGTCATCATTGTTCTT+AGG 0.279548 6_2:+79651910 Msa0930550:CDS
AAGATTGAGCTTAAATAATA+AGG 0.285883 6_2:-79652226 None:intergenic
ATGGAGATACTAGCTTATTT+GGG 0.292207 6_2:-79651936 None:intergenic
GAGTTGGATAAAAGCGGTTT+TGG 0.304648 6_2:+79648357 Msa0930550:five_prime_UTR
AATAGTTTAGTATTGTTTGC+TGG 0.372271 6_2:+79652269 Msa0930550:three_prime_UTR
AAAAGCTTCTAGGCCGTGTC+AGG 0.374760 6_2:+79651646 Msa0930550:CDS
TGGATCAAAACCCTAGTTTA+CGG 0.383983 6_2:-79648404 None:intergenic
CTTCTGCTTCTGCTTCTGCT+TGG 0.384025 6_2:+79648548 Msa0930550:five_prime_UTR
TTGGTGGAGGATCTTGATTT+AGG 0.384590 6_2:+79652065 Msa0930550:three_prime_UTR
TTCTTTCCTTTACCAGTCTT+AGG 0.400785 6_2:-79651831 None:intergenic
CTGCTTCTGCTTCTGCTTGG+AGG 0.412952 6_2:+79648551 Msa0930550:five_prime_UTR
ATACCTCAGTCCACATTTCC+CGG 0.427349 6_2:-79651707 None:intergenic
ATATGGTTCTTGAAAATGTC+CGG 0.429724 6_2:+79651688 Msa0930550:CDS
CTCAATTGTTACAGGCTGTC+GGG 0.432771 6_2:+79651431 Msa0930550:intron
ATCAGCAAAATGTTCCTTCG+TGG 0.434985 6_2:+79651885 Msa0930550:CDS
TCGTTGTTGACCCGTAAACT+AGG 0.456360 6_2:+79648393 Msa0930550:five_prime_UTR
ATGGAATTCGAGAACCGATT+CGG 0.469046 6_2:-79648511 None:intergenic
GGATCAAAACCCTAGTTTAC+GGG 0.469563 6_2:-79648403 None:intergenic
ACTTTGAACATAACTGAACT+AGG 0.470561 6_2:+79648462 Msa0930550:five_prime_UTR
GAACGAAAATGGGGCTGAGT+TGG 0.475275 6_2:+79648341 None:intergenic
AACAAGATCAAACAAATTTG+CGG 0.475374 6_2:+79652102 Msa0930550:three_prime_UTR
CCATCCCTCTGTTGAAGTAG+TGG 0.478064 6_2:+79651955 Msa0930550:three_prime_UTR
TAGCTTACATCTTCTTCCAT+TGG 0.480536 6_2:-79650778 None:intergenic
CATCCCTCTGTTGAAGTAGT+GGG 0.492663 6_2:+79651956 Msa0930550:three_prime_UTR
CAGGTGCCTAAGACTGGTAA+AGG 0.497162 6_2:+79651825 Msa0930550:intron
TTACATCTTCTTCCATTGGC+CGG 0.497216 6_2:-79650774 None:intergenic
TTGAAAATGTCCGGGAAATG+TGG 0.500328 6_2:+79651697 Msa0930550:CDS
CGTTGTTGACCCGTAAACTA+GGG 0.505968 6_2:+79648394 Msa0930550:five_prime_UTR
CTCATCATGAGTACAGATAG+TGG 0.509132 6_2:-79651484 None:intergenic
ATGTATCAGGTGCCTAAGAC+TGG 0.510734 6_2:+79651819 Msa0930550:intron
TTGAAACGTGGAGAAATTGG+TGG 0.515121 6_2:+79652049 Msa0930550:three_prime_UTR
AGAAGCAGATCTGAGATGAA+TGG 0.515312 6_2:-79648530 None:intergenic
TTGAGCTTAAATAATAAGGA+AGG 0.521594 6_2:-79652222 None:intergenic
TATAAAGAGTAGATCCGAAT+CGG 0.534917 6_2:+79648497 Msa0930550:five_prime_UTR
GAAGGCTCAGCCAGTCAACA+AGG 0.537512 6_2:+79651854 Msa0930550:CDS
CTTTGAACATAACTGAACTA+GGG 0.537954 6_2:+79648463 Msa0930550:five_prime_UTR
TGTAGTGATGATGTATGAAA+TGG 0.537975 6_2:+79652147 Msa0930550:three_prime_UTR
ATGAAACGATCCTTGTTGAC+TGG 0.541044 6_2:-79651864 None:intergenic
ACTCAATTGTTACAGGCTGT+CGG 0.551091 6_2:+79651430 Msa0930550:intron
TTCTTCTTGAAGCCGGCCAA+TGG 0.551208 6_2:+79650762 Msa0930550:intron
GACTGGTAAAGGAAAGAAGA+AGG 0.552162 6_2:+79651836 Msa0930550:CDS
TGTCGGGAAGAATGAGGAAG+AGG 0.555164 6_2:+79651447 Msa0930550:CDS
TATGGTTCTTGAAAATGTCC+GGG 0.564509 6_2:+79651689 Msa0930550:CDS
TGTATGAAATGGTTCGACTC+TGG 0.568262 6_2:+79652158 Msa0930550:three_prime_UTR
GTCTATCAAAAGCCCTGACA+CGG 0.582472 6_2:-79651659 None:intergenic
CCACAGACTCTTCCAAAATG+AGG 0.586420 6_2:+79650642 Msa0930550:exon
TGGAGATACTAGCTTATTTG+GGG 0.598626 6_2:-79651935 None:intergenic
AAACGTGGAGAAATTGGTGG+AGG 0.605244 6_2:+79652052 Msa0930550:three_prime_UTR
ACAGGCTGTCGGGAAGAATG+AGG 0.617342 6_2:+79651441 Msa0930550:intron
GAGCCCACTACTTCAACAGA+GGG 0.639565 6_2:-79651959 None:intergenic
GAGGAAGAGGAATTCAACAC+TGG 0.639973 6_2:+79651460 Msa0930550:CDS
GGGGCTGAGTTGGATAAAAG+CGG 0.647258 6_2:+79648351 Msa0930550:five_prime_UTR
AGAGCCCACTACTTCAACAG+AGG 0.653640 6_2:-79651960 None:intergenic
CCACTACTTCAACAGAGGGA+TGG 0.654111 6_2:-79651955 None:intergenic
AAAGCTTCTAGGCCGTGTCA+GGG 0.669612 6_2:+79651647 Msa0930550:CDS
TGTCCGGGAAATGTGGACTG+AGG 0.699661 6_2:+79651704 Msa0930550:CDS
TGATGACAGAATCTCCACGA+AGG 0.746510 6_2:-79651899 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTTAGATTGATTTTA+TGG + chr6_2:79649892-79649911 Msa0930550:intron 10.0%
!!! TTTTATTATGATTTTATAGT+TGG + chr6_2:79650153-79650172 Msa0930550:intron 10.0%
!! AAATCTAAATTAAATTACAA+AGG - chr6_2:79650511-79650530 None:intergenic 10.0%
!!! TTTTTTTTTTTAAAAAACAG+AGG + chr6_2:79651285-79651304 Msa0930550:intron 10.0%
!! AAATAAGTTTCGAAATTAAA+AGG - chr6_2:79648607-79648626 None:intergenic 15.0%
!!! ATTTCATTTTTGTTGAAATT+AGG + chr6_2:79648623-79648642 Msa0930550:intron 15.0%
!! AAAAAAAAAAAAAGTCTTTG+AGG + chr6_2:79648691-79648710 Msa0930550:intron 15.0%
!! CAATACAATTAAAAGAAAAT+TGG - chr6_2:79648852-79648871 None:intergenic 15.0%
!!! CAATTTTCTTTTAATTGTAT+TGG + chr6_2:79648850-79648869 Msa0930550:intron 15.0%
!!! TTTTTTTTTTAGAATTGTTC+AGG + chr6_2:79649223-79649242 Msa0930550:intron 15.0%
!! CTTCAAAAAAAAAATTACAA+AGG - chr6_2:79650478-79650497 None:intergenic 15.0%
!!! TACTAAACTATTTATACTTT+TGG - chr6_2:79652261-79652280 None:intergenic 15.0%
!! ACAATAAAAAACGCAAAAAA+TGG - chr6_2:79648807-79648826 None:intergenic 20.0%
!!! TTTTTATTGTGATTGTTGTT+TGG + chr6_2:79648817-79648836 Msa0930550:intron 20.0%
!! AAAAAAAAAACACAAGAACA+GGG - chr6_2:79649213-79649232 None:intergenic 20.0%
!! TAAAAAAAAAACACAAGAAC+AGG - chr6_2:79649214-79649233 None:intergenic 20.0%
!! TGAATATGTGTTTATATAAG+CGG + chr6_2:79649283-79649302 Msa0930550:intron 20.0%
!! AATATTCTTCTTAGAATTTG+TGG - chr6_2:79649515-79649534 None:intergenic 20.0%
!! AAAAGATCATAGTAAAACAT+GGG - chr6_2:79649776-79649795 None:intergenic 20.0%
!! AAAAAGATCATAGTAAAACA+TGG - chr6_2:79649777-79649796 None:intergenic 20.0%
!!! TTTGTTGTTTTGCAATTTTA+CGG + chr6_2:79649794-79649813 Msa0930550:intron 20.0%
!!! TGTTTTATCATTACATAACT+TGG + chr6_2:79651076-79651095 Msa0930550:intron 20.0%
!!! ACTTTGACTTTAATGTTAAA+TGG + chr6_2:79651363-79651382 Msa0930550:intron 20.0%
!! AAGATTGAGCTTAAATAATA+AGG - chr6_2:79652229-79652248 None:intergenic 20.0%
! AAAATCAAAGATAACACAAG+TGG - chr6_2:79648660-79648679 None:intergenic 25.0%
! AAAATGGAAAACATAAGCAA+AGG - chr6_2:79648791-79648810 None:intergenic 25.0%
!! TGTTAAGAATTTTCCCTTAT+AGG + chr6_2:79648889-79648908 Msa0930550:intron 25.0%
!! AGAATTAGCTTATCCTATAA+GGG - chr6_2:79648905-79648924 None:intergenic 25.0%
!! AAGAATTAGCTTATCCTATA+AGG - chr6_2:79648906-79648925 None:intergenic 25.0%
!!! AATTCTTTTGTTTACTCCAT+AGG + chr6_2:79648919-79648938 Msa0930550:intron 25.0%
!! ATTAGTTTAATCATCATCAG+AGG - chr6_2:79649113-79649132 None:intergenic 25.0%
! GAATATGTGTTTATATAAGC+GGG + chr6_2:79649284-79649303 Msa0930550:intron 25.0%
! AATATGTGTTTATATAAGCG+GGG + chr6_2:79649285-79649304 Msa0930550:intron 25.0%
! TTAAGTGTAATTACAAGTGT+TGG - chr6_2:79649404-79649423 None:intergenic 25.0%
!!! ATTTGTTATTCACGATTTTC+AGG + chr6_2:79649866-79649885 Msa0930550:intron 25.0%
! CATAATAAAAACACAAGAGA+TGG - chr6_2:79650143-79650162 None:intergenic 25.0%
! TGTATCTTCTGTTAAAATCA+AGG - chr6_2:79650930-79650949 None:intergenic 25.0%
!!! ATTTTAACAGAAGATACACT+TGG + chr6_2:79650932-79650951 Msa0930550:intron 25.0%
!! TCAATATGCTCTATTTGTTT+TGG + chr6_2:79651023-79651042 Msa0930550:intron 25.0%
! ATCATTACATAACTTGGTAA+AGG + chr6_2:79651082-79651101 Msa0930550:intron 25.0%
!!! ATTTGAATTTACTTTTCTGC+TGG + chr6_2:79651770-79651789 Msa0930550:intron 25.0%
! AACAAGATCAAACAAATTTG+CGG + chr6_2:79652102-79652121 Msa0930550:three_prime_UTR 25.0%
! TTGAGCTTAAATAATAAGGA+AGG - chr6_2:79652225-79652244 None:intergenic 25.0%
!!! AACTATTTATACTTTTGGAG+CGG - chr6_2:79652256-79652275 None:intergenic 25.0%
! AATAGTTTAGTATTGTTTGC+TGG + chr6_2:79652269-79652288 Msa0930550:three_prime_UTR 25.0%
!!! TTTGTTTTGTATGACAACGA+TGG - chr6_2:79648427-79648446 None:intergenic 30.0%
ACTTTGAACATAACTGAACT+AGG + chr6_2:79648462-79648481 Msa0930550:five_prime_UTR 30.0%
CTTTGAACATAACTGAACTA+GGG + chr6_2:79648463-79648482 Msa0930550:five_prime_UTR 30.0%
TATAAAGAGTAGATCCGAAT+CGG + chr6_2:79648497-79648516 Msa0930550:five_prime_UTR 30.0%
TAATCCATGCGGAAATTATA+TGG + chr6_2:79648748-79648767 Msa0930550:intron 30.0%
!! AATTTAGTGTTTTTGCTCCA+AGG + chr6_2:79648962-79648981 Msa0930550:intron 30.0%
!! AATTTAGTGTTTTTGCTCCA+AGG + chr6_2:79648962-79648981 Msa0930550:intron 30.0%
TATTCCATGCGGAAAATATA+TGG + chr6_2:79649151-79649170 Msa0930550:intron 30.0%
CACATATTCAACACATATAC+AGG - chr6_2:79649273-79649292 None:intergenic 30.0%
ATATGTGTTTATATAAGCGG+GGG + chr6_2:79649286-79649305 Msa0930550:intron 30.0%
TCTTCTTAGAATTTGTGGTT+GGG - chr6_2:79649510-79649529 None:intergenic 30.0%
TTCTTCTTAGAATTTGTGGT+TGG - chr6_2:79649511-79649530 None:intergenic 30.0%
GGAAATAGGAACAAAAATCA+AGG - chr6_2:79649580-79649599 None:intergenic 30.0%
! TTTGTTCCTATTTCCTCTTT+TGG + chr6_2:79649585-79649604 Msa0930550:intron 30.0%
! TTGTCCATGCAGAAATTTTA+TGG + chr6_2:79649684-79649703 Msa0930550:intron 30.0%
! ATCATCAGAGGATTTTTGAA+TGG - chr6_2:79650007-79650026 None:intergenic 30.0%
ATTTGTTTGATCATCATCAG+AGG - chr6_2:79650019-79650038 None:intergenic 30.0%
TTGTCCATGTGGAAATTATA+TGG + chr6_2:79650057-79650076 Msa0930550:intron 30.0%
AATAAAAACACAAGAGATGG+AGG - chr6_2:79650140-79650159 None:intergenic 30.0%
!! AAATTTTAGCAGTTGCTGTT+TGG + chr6_2:79650193-79650212 Msa0930550:intron 30.0%
!!! TTGTTCCAATTGGCTTTTTT+TGG + chr6_2:79650220-79650239 Msa0930550:intron 30.0%
TATAATTTGATCATCCTCAG+AGG - chr6_2:79650325-79650344 None:intergenic 30.0%
TTTAACATGCGGAAATTTCA+TGG + chr6_2:79650362-79650381 Msa0930550:intron 30.0%
ATCATACTGAAACAGAGATT+TGG - chr6_2:79650447-79650466 None:intergenic 30.0%
TCAACTTCATTAGAGGAATT+TGG + chr6_2:79650609-79650628 Msa0930550:five_prime_UTR 30.0%
! AGAGAATACATACCTCATTT+TGG - chr6_2:79650657-79650676 None:intergenic 30.0%
! TTGATTTTTCTTCTTGAAGC+CGG + chr6_2:79650755-79650774 Msa0930550:intron 30.0%
TTTGCATTATGATCCTGATA+TGG + chr6_2:79650833-79650852 Msa0930550:intron 30.0%
TGTTCTATTTAGTGCACATT+TGG + chr6_2:79650871-79650890 Msa0930550:intron 30.0%
TGAAATCACAATTCTGAATC+AGG - chr6_2:79651049-79651068 None:intergenic 30.0%
GAAGAAAGAAAAAAGAAGAC+TGG - chr6_2:79651319-79651338 None:intergenic 30.0%
! GATTTTACAAACACTTATGC+AGG + chr6_2:79651599-79651618 Msa0930550:intron 30.0%
! TTTTGATAGACACTGCAATA+TGG + chr6_2:79651671-79651690 Msa0930550:CDS 30.0%
ATATGGTTCTTGAAAATGTC+CGG + chr6_2:79651688-79651707 Msa0930550:CDS 30.0%
ATGCAAATTACTTCTCTACT+TGG + chr6_2:79651744-79651763 Msa0930550:intron 30.0%
ATTCTGTCATCATTGTTCTT+AGG + chr6_2:79651910-79651929 Msa0930550:CDS 30.0%
! ATGGAGATACTAGCTTATTT+GGG - chr6_2:79651939-79651958 None:intergenic 30.0%
CGCATTATTGGTATGAAATA+TGG + chr6_2:79652003-79652022 Msa0930550:three_prime_UTR 30.0%
! AGCCAAATTTTATTGAAACG+TGG + chr6_2:79652037-79652056 Msa0930550:three_prime_UTR 30.0%
CTCCACGTTTCAATAAAATT+TGG - chr6_2:79652042-79652061 None:intergenic 30.0%
TTATTGAAACGTGGAGAAAT+TGG + chr6_2:79652046-79652065 Msa0930550:three_prime_UTR 30.0%
! TGTAGTGATGATGTATGAAA+TGG + chr6_2:79652147-79652166 Msa0930550:three_prime_UTR 30.0%
!!! ACTATTTATACTTTTGGAGC+GGG - chr6_2:79652255-79652274 None:intergenic 30.0%
! AGTTGGATAAAAGCGGTTTT+GGG + chr6_2:79648358-79648377 Msa0930550:five_prime_UTR 35.0%
TGGATCAAAACCCTAGTTTA+CGG - chr6_2:79648407-79648426 None:intergenic 35.0%
AGGCATGAAGTTGAAGAAAA+TGG - chr6_2:79648587-79648606 None:intergenic 35.0%
CATCAGAATATCATGACCTA+TGG - chr6_2:79648938-79648957 None:intergenic 35.0%
ATCATGACCTATGGAGTAAA+CGG - chr6_2:79648929-79648948 None:intergenic 35.0%
CATCAGAATATCATGACCTA+TGG - chr6_2:79648938-79648957 None:intergenic 35.0%
TCATCAGAGGCTTATTGAAT+GGG - chr6_2:79649100-79649119 None:intergenic 35.0%
ATCATCAGAGGCTTATTGAA+TGG - chr6_2:79649101-79649120 None:intergenic 35.0%
! TATTTTCCGCATGGAATACT+CGG - chr6_2:79649149-79649168 None:intergenic 35.0%
AAAAAACACAAGAACAGGGA+TGG - chr6_2:79649209-79649228 None:intergenic 35.0%
!! GTAATTACAAGTGTTGGTGT+CGG - chr6_2:79649398-79649417 None:intergenic 35.0%
GAATCACCAAAAGAGGAAAT+AGG - chr6_2:79649594-79649613 None:intergenic 35.0%
TCAGAGGCTTTACATTTAAG+TGG - chr6_2:79649630-79649649 None:intergenic 35.0%
ACTTAAATGTAAAGCCTCTG+AGG + chr6_2:79649629-79649648 Msa0930550:intron 35.0%
ATTTGTTTGATCATCCTCAG+AGG - chr6_2:79649646-79649665 None:intergenic 35.0%
GTCTCCATAAAATTTCTGCA+TGG - chr6_2:79649691-79649710 None:intergenic 35.0%
! GATTTTTGAATGGTGGTTTC+AGG - chr6_2:79649997-79650016 None:intergenic 35.0%
! ATCAGAGGATTTTTGAATGG+TGG - chr6_2:79650004-79650023 None:intergenic 35.0%
TAATTTCCACATGGACAACT+CGG - chr6_2:79650055-79650074 None:intergenic 35.0%
GTCTCCATATAATTTCCACA+TGG - chr6_2:79650064-79650083 None:intergenic 35.0%
! GTTTGGTTTCTTGTTCCAAT+TGG + chr6_2:79648834-79648853 Msa0930550:intron 35.0%
AAGTGCCAAAAAAAGCCAAT+TGG - chr6_2:79650228-79650247 None:intergenic 35.0%
GTTCTTCTGAGTTTAACATG+CGG + chr6_2:79650351-79650370 Msa0930550:intron 35.0%
TGCAGTTTCAACTTCATTAG+AGG + chr6_2:79650602-79650621 Msa0930550:intron 35.0%
TAGCTTACATCTTCTTCCAT+TGG - chr6_2:79650781-79650800 None:intergenic 35.0%
ACAGATATGCAATCCATATC+AGG - chr6_2:79650849-79650868 None:intergenic 35.0%
ATTTGGATTGACCTTGAGTT+TGG + chr6_2:79650888-79650907 Msa0930550:intron 35.0%
TGAGTTTGACTAAATCATGC+TGG - chr6_2:79650979-79650998 None:intergenic 35.0%
AAAAAAGAAGACTGGACTTC+AGG - chr6_2:79651311-79651330 None:intergenic 35.0%
CTGACATACTCAATTGTTAC+AGG + chr6_2:79651423-79651442 Msa0930550:intron 35.0%
TACTGTGTGTTCTTCTTGAT+CGG + chr6_2:79651554-79651573 Msa0930550:intron 35.0%
CGTAACAACAAAAAGCTTCT+AGG + chr6_2:79651636-79651655 Msa0930550:CDS 35.0%
TATGGTTCTTGAAAATGTCC+GGG + chr6_2:79651689-79651708 Msa0930550:CDS 35.0%
!! TGAATTTACTTTTCTGCTGG+TGG + chr6_2:79651773-79651792 Msa0930550:intron 35.0%
ATGTTCTGCTTGTATGTATC+AGG + chr6_2:79651806-79651825 Msa0930550:intron 35.0%
TTCTTTCCTTTACCAGTCTT+AGG - chr6_2:79651834-79651853 None:intergenic 35.0%
! TGGAGATACTAGCTTATTTG+GGG - chr6_2:79651938-79651957 None:intergenic 35.0%
! GATGGAGATACTAGCTTATT+TGG - chr6_2:79651940-79651959 None:intergenic 35.0%
!!! AAGTAGTGGGCTCTTATTTT+TGG + chr6_2:79651969-79651988 Msa0930550:three_prime_UTR 35.0%
!!! GCTTTGTTTTCTCGCATTAT+TGG + chr6_2:79651991-79652010 Msa0930550:three_prime_UTR 35.0%
!!! CTATTTATACTTTTGGAGCG+GGG - chr6_2:79652254-79652273 None:intergenic 35.0%
! GAGTTGGATAAAAGCGGTTT+TGG + chr6_2:79648357-79648376 Msa0930550:five_prime_UTR 40.0%
GGATCAAAACCCTAGTTTAC+GGG - chr6_2:79648406-79648425 None:intergenic 40.0%
ATGGAATTCGAGAACCGATT+CGG - chr6_2:79648514-79648533 None:intergenic 40.0%
! AGAAGCAGATCTGAGATGAA+TGG - chr6_2:79648533-79648552 None:intergenic 40.0%
GTTCTTCTGAGTAATCCATG+CGG + chr6_2:79648737-79648756 Msa0930550:intron 40.0%
GTCTCCATATAATTTCCGCA+TGG - chr6_2:79648755-79648774 None:intergenic 40.0%
AAGCAAAGGCTATTCAGTTC+AGG - chr6_2:79648777-79648796 None:intergenic 40.0%
!!! TTTGCTCCAAGGTTTTTAGC+TGG + chr6_2:79648973-79648992 Msa0930550:intron 40.0%
TCATGACCTATGGAGTAAAC+GGG - chr6_2:79648928-79648947 None:intergenic 40.0%
!!! TTTGCTCCAAGGTTTTTAGC+TGG + chr6_2:79648973-79648992 Msa0930550:intron 40.0%
GCTTATTGAATGGGGTTTGA+AGG - chr6_2:79649091-79649110 None:intergenic 40.0%
CATCAGAGGCTTATTGAATG+GGG - chr6_2:79649099-79649118 None:intergenic 40.0%
! GTCTCCATATATTTTCCGCA+TGG - chr6_2:79649158-79649177 None:intergenic 40.0%
TAAGAAAGCCTCTTCAGTTC+AGG - chr6_2:79649180-79649199 None:intergenic 40.0%
TCTTTGTATTAGGTGTGTCC+CGG + chr6_2:79649347-79649366 Msa0930550:intron 40.0%
AAGGCAAGAATCACCAAAAG+AGG - chr6_2:79649601-79649620 None:intergenic 40.0%
!! TCTTTTGGTGATTCTTGCCT+TGG + chr6_2:79649600-79649619 Msa0930550:intron 40.0%
TACATTTAAGTGGTGCTCCA+AGG - chr6_2:79649620-79649639 None:intergenic 40.0%
TTAACAAGCCTCTTCAGTTC+AGG - chr6_2:79649713-79649732 None:intergenic 40.0%
TTAGCAAGCCTATTCAGTTC+AGG - chr6_2:79649823-79649842 None:intergenic 40.0%
! CTCAAAGCTTTAATGCGAGT+AGG + chr6_2:79649934-79649953 Msa0930550:intron 40.0%
!! ATGCGAGTAGGTCTTGTTTA+AGG + chr6_2:79649946-79649965 Msa0930550:intron 40.0%
ATAGAAAGCCTCTTCAGTTC+AGG - chr6_2:79650086-79650105 None:intergenic 40.0%
GGATATGACACTAGGGTAAT+GGG - chr6_2:79650119-79650138 None:intergenic 40.0%
AGGATATGACACTAGGGTAA+TGG - chr6_2:79650120-79650139 None:intergenic 40.0%
AGATGGAGGATATGACACTA+GGG - chr6_2:79650126-79650145 None:intergenic 40.0%
AAACAGAGATTTGGAAGCCA+TGG - chr6_2:79650438-79650457 None:intergenic 40.0%
ACAAGAAAGCAATGAGCTGA+AGG - chr6_2:79650537-79650556 None:intergenic 40.0%
TGAGGTATGTATTCTCTTGC+TGG + chr6_2:79650660-79650679 Msa0930550:intron 40.0%
TTACATCTTCTTCCATTGGC+CGG - chr6_2:79650777-79650796 None:intergenic 40.0%
! TGAAAGTCAGCTTTGTGTTG+TGG + chr6_2:79651169-79651188 Msa0930550:intron 40.0%
TCTTTCTTCATGCGTCACAA+TGG + chr6_2:79651330-79651349 Msa0930550:intron 40.0%
ACTCAATTGTTACAGGCTGT+CGG + chr6_2:79651430-79651449 Msa0930550:intron 40.0%
CTCATCATGAGTACAGATAG+TGG - chr6_2:79651487-79651506 None:intergenic 40.0%
GAGTGTGAAAAACAACACAC+AGG + chr6_2:79651504-79651523 Msa0930550:CDS 40.0%
TTGAAAATGTCCGGGAAATG+TGG + chr6_2:79651697-79651716 Msa0930550:CDS 40.0%
GACTGGTAAAGGAAAGAAGA+AGG + chr6_2:79651836-79651855 Msa0930550:CDS 40.0%
ATGAAACGATCCTTGTTGAC+TGG - chr6_2:79651867-79651886 None:intergenic 40.0%
ATCAGCAAAATGTTCCTTCG+TGG + chr6_2:79651885-79651904 Msa0930550:CDS 40.0%
TTGAAACGTGGAGAAATTGG+TGG + chr6_2:79652049-79652068 Msa0930550:three_prime_UTR 40.0%
TTGGTGGAGGATCTTGATTT+AGG + chr6_2:79652065-79652084 Msa0930550:three_prime_UTR 40.0%
TGTATGAAATGGTTCGACTC+TGG + chr6_2:79652158-79652177 Msa0930550:three_prime_UTR 40.0%
TCGTTGTTGACCCGTAAACT+AGG + chr6_2:79648393-79648412 Msa0930550:five_prime_UTR 45.0%
CGTTGTTGACCCGTAAACTA+GGG + chr6_2:79648394-79648413 Msa0930550:five_prime_UTR 45.0%
CCTAGACCAGCTAAAAACCT+TGG - chr6_2:79648982-79649001 None:intergenic 45.0%
!!! CCAAGGTTTTTAGCTGGTCT+AGG + chr6_2:79648979-79648998 Msa0930550:intron 45.0%
ATGGAGTAAACGGGGTTTGA+AGG - chr6_2:79649005-79649024 None:intergenic 45.0%
TCAAACCCCGTTTACTCCAT+AGG + chr6_2:79649005-79649024 Msa0930550:intron 45.0%
CATGACCTATGGAGTAAACG+GGG - chr6_2:79649013-79649032 None:intergenic 45.0%
CCTAGACCAGCTAAAAACCT+TGG - chr6_2:79648982-79649001 None:intergenic 45.0%
!!! CCAAGGTTTTTAGCTGGTCT+AGG + chr6_2:79648979-79648998 Msa0930550:intron 45.0%
GTTCTTCCGAGTATTCCATG+CGG + chr6_2:79649140-79649159 Msa0930550:intron 45.0%
TATGGAGACCTGAACTGAAG+AGG + chr6_2:79649169-79649188 Msa0930550:intron 45.0%
GTCCCACATTAGTTGAGAGA+TGG + chr6_2:79649247-79649266 Msa0930550:intron 45.0%
GGCCATCTCTCAACTAATGT+GGG - chr6_2:79649252-79649271 None:intergenic 45.0%
AGGCCATCTCTCAACTAATG+TGG - chr6_2:79649253-79649272 None:intergenic 45.0%
GCACGGACACTCTTTGTATT+AGG + chr6_2:79649337-79649356 Msa0930550:intron 45.0%
TGAGCTATGAAGCACGGATA+CGG - chr6_2:79649459-79649478 None:intergenic 45.0%
! TAGCTCACAAGCCAGTTTTG+TGG + chr6_2:79649472-79649491 Msa0930550:intron 45.0%
!! AGCTCACAAGCCAGTTTTGT+GGG + chr6_2:79649473-79649492 Msa0930550:intron 45.0%
CTAACACAACCCCACAAAAC+TGG - chr6_2:79649486-79649505 None:intergenic 45.0%
!! CAGTTTTGTGGGGTTGTGTT+AGG + chr6_2:79649484-79649503 Msa0930550:intron 45.0%
TATGGAGACCTGAACTGAAG+AGG + chr6_2:79649169-79649188 Msa0930550:intron 45.0%
TTCTCCTTTCCTTTCTGCCA+TGG + chr6_2:79649741-79649760 Msa0930550:intron 45.0%
AAAGCCATGGCAGAAAGGAA+AGG - chr6_2:79649748-79649767 None:intergenic 45.0%
ATGTGAAAGCCATGGCAGAA+AGG - chr6_2:79649753-79649772 None:intergenic 45.0%
AACATGGGATGTGAAAGCCA+TGG - chr6_2:79649761-79649780 None:intergenic 45.0%
TACGGAGACCTGAACTGAAT+AGG + chr6_2:79649812-79649831 Msa0930550:intron 45.0%
TATGGAGACCTGAACTGAAG+AGG + chr6_2:79649169-79649188 Msa0930550:intron 45.0%
!! ATGACACTAGGGTAATGGGA+AGG - chr6_2:79650115-79650134 None:intergenic 45.0%
GAGATGGAGGATATGACACT+AGG - chr6_2:79650127-79650146 None:intergenic 45.0%
! ATACTCAAGCGGGGTTTTCA+AGG - chr6_2:79650300-79650319 None:intergenic 45.0%
CAAGTATGCCTCTTCAGTTC+AGG - chr6_2:79650391-79650410 None:intergenic 45.0%
ATTTGGAAGCCATGGCACAA+AGG - chr6_2:79650430-79650449 None:intergenic 45.0%
!! CCTCATTTTGGAAGAGTCTG+TGG - chr6_2:79650645-79650664 None:intergenic 45.0%
CCACAGACTCTTCCAAAATG+AGG + chr6_2:79650642-79650661 Msa0930550:exon 45.0%
TGGATTGACCTTGAGTTTGG+CGG + chr6_2:79650891-79650910 Msa0930550:intron 45.0%
CTCAATTGTTACAGGCTGTC+GGG + chr6_2:79651431-79651450 Msa0930550:intron 45.0%
GAGGAAGAGGAATTCAACAC+TGG + chr6_2:79651460-79651479 Msa0930550:CDS 45.0%
GTCTATCAAAAGCCCTGACA+CGG - chr6_2:79651662-79651681 None:intergenic 45.0%
ATACCTCAGTCCACATTTCC+CGG - chr6_2:79651710-79651729 None:intergenic 45.0%
ATGTATCAGGTGCCTAAGAC+TGG + chr6_2:79651819-79651838 Msa0930550:intron 45.0%
TGATGACAGAATCTCCACGA+AGG - chr6_2:79651902-79651921 None:intergenic 45.0%
CATCCCTCTGTTGAAGTAGT+GGG + chr6_2:79651956-79651975 Msa0930550:three_prime_UTR 45.0%
AAACGTGGAGAAATTGGTGG+AGG + chr6_2:79652052-79652071 Msa0930550:three_prime_UTR 45.0%
!! AAAAAAAAAAAAAAAAAAGA+TGG - chr6_2:79651273-79651292 None:intergenic 5.0%
! GGGGCTGAGTTGGATAAAAG+CGG + chr6_2:79648351-79648370 Msa0930550:five_prime_UTR 50.0%
CTTCTGCTTCTGCTTCTGCT+TGG + chr6_2:79648548-79648567 Msa0930550:five_prime_UTR 50.0%
! TCATAGCTTGTGAGGCATGG+AGG - chr6_2:79649316-79649335 None:intergenic 50.0%
! GCTTCATAGCTTGTGAGGCA+TGG - chr6_2:79649319-79649338 None:intergenic 50.0%
GCCTCACAAGCTATGAAGCA+CGG + chr6_2:79649320-79649339 Msa0930550:intron 50.0%
! TCCGTGCTTCATAGCTTGTG+AGG - chr6_2:79649324-79649343 None:intergenic 50.0%
TATTAGGTGTGTCCCGGTGT+CGG + chr6_2:79649353-79649372 Msa0930550:intron 50.0%
GGCTTGTGAGCTATGAAGCA+CGG - chr6_2:79649465-79649484 None:intergenic 50.0%
!! GCTCACAAGCCAGTTTTGTG+GGG + chr6_2:79649474-79649493 Msa0930550:intron 50.0%
GTTCTTCCGAGTTGTCCATG+TGG + chr6_2:79650046-79650065 Msa0930550:intron 50.0%
TCAGAGGCTATACTCAAGCG+GGG - chr6_2:79650309-79650328 None:intergenic 50.0%
CTCAGAGGCTATACTCAAGC+GGG - chr6_2:79650310-79650329 None:intergenic 50.0%
CCTCAGAGGCTATACTCAAG+CGG - chr6_2:79650311-79650330 None:intergenic 50.0%
CATGGAGACCTGAACTGAAG+AGG + chr6_2:79650380-79650399 Msa0930550:intron 50.0%
TTTCCCCTTCCTTTGTGCCA+TGG + chr6_2:79650418-79650437 Msa0930550:intron 50.0%
AAGCCATGGCACAAAGGAAG+GGG - chr6_2:79650424-79650443 None:intergenic 50.0%
GAAGCCATGGCACAAAGGAA+GGG - chr6_2:79650425-79650444 None:intergenic 50.0%
TTCTTCTTGAAGCCGGCCAA+TGG + chr6_2:79650762-79650781 Msa0930550:intron 50.0%
CCAAGATTCCGCCAAACTCA+AGG - chr6_2:79650902-79650921 None:intergenic 50.0%
CCTTGAGTTTGGCGGAATCT+TGG + chr6_2:79650899-79650918 Msa0930550:intron 50.0%
TGTCGGGAAGAATGAGGAAG+AGG + chr6_2:79651447-79651466 Msa0930550:CDS 50.0%
AAAAGCTTCTAGGCCGTGTC+AGG + chr6_2:79651646-79651665 Msa0930550:CDS 50.0%
AAAGCTTCTAGGCCGTGTCA+GGG + chr6_2:79651647-79651666 Msa0930550:CDS 50.0%
CAGGTGCCTAAGACTGGTAA+AGG + chr6_2:79651825-79651844 Msa0930550:intron 50.0%
CCACTACTTCAACAGAGGGA+TGG - chr6_2:79651958-79651977 None:intergenic 50.0%
CCATCCCTCTGTTGAAGTAG+TGG + chr6_2:79651955-79651974 Msa0930550:three_prime_UTR 50.0%
GAGCCCACTACTTCAACAGA+GGG - chr6_2:79651962-79651981 None:intergenic 50.0%
AGAGCCCACTACTTCAACAG+AGG - chr6_2:79651963-79651982 None:intergenic 50.0%
!!! AAGCGGTTTTGGGCTGCGAA+TGG + chr6_2:79648368-79648387 Msa0930550:five_prime_UTR 55.0%
!!! AGCGGTTTTGGGCTGCGAAT+GGG + chr6_2:79648369-79648388 Msa0930550:five_prime_UTR 55.0%
CTGCTTCTGCTTCTGCTTGG+AGG + chr6_2:79648551-79648570 Msa0930550:five_prime_UTR 55.0%
TGAAGCACGGATACGGACAC+TGG - chr6_2:79649452-79649471 None:intergenic 55.0%
CCGCTTGAGTATAGCCTCTG+AGG + chr6_2:79650308-79650327 Msa0930550:intron 55.0%
GGAAGCCATGGCACAAAGGA+AGG - chr6_2:79650426-79650445 None:intergenic 55.0%
ACAGGCTGTCGGGAAGAATG+AGG + chr6_2:79651441-79651460 Msa0930550:intron 55.0%
! TGTCCGGGAAATGTGGACTG+AGG + chr6_2:79651704-79651723 Msa0930550:CDS 55.0%
GAAGGCTCAGCCAGTCAACA+AGG + chr6_2:79651854-79651873 Msa0930550:CDS 55.0%
ACACGACACGTGTCCGACAC+CGG - chr6_2:79649369-79649388 None:intergenic 60.0%
CACGACACGTGTCCGACACC+GGG - chr6_2:79649368-79649387 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 79648344 79652297 79648344 ID=Msa0930550;Name=Msa0930550
chr6_2 mRNA 79648344 79652297 79648344 ID=Msa0930550-mRNA-1;Parent=Msa0930550;Name=Msa0930550-mRNA-1;_AED=0.08;_eAED=0.08;_QI=281|1|1|1|0.6|0.66|6|353|108
chr6_2 exon 79648344 79648572 79648344 ID=Msa0930550-mRNA-1:exon:13742;Parent=Msa0930550-mRNA-1
chr6_2 exon 79650607 79650663 79650607 ID=Msa0930550-mRNA-1:exon:13743;Parent=Msa0930550-mRNA-1
chr6_2 exon 79650774 79650792 79650774 ID=Msa0930550-mRNA-1:exon:13744;Parent=Msa0930550-mRNA-1
chr6_2 exon 79651445 79651525 79651445 ID=Msa0930550-mRNA-1:exon:13745;Parent=Msa0930550-mRNA-1
chr6_2 exon 79651621 79651725 79651621 ID=Msa0930550-mRNA-1:exon:13746;Parent=Msa0930550-mRNA-1
chr6_2 exon 79651828 79652297 79651828 ID=Msa0930550-mRNA-1:exon:13747;Parent=Msa0930550-mRNA-1
chr6_2 five_prime_UTR 79648344 79648572 79648344 ID=Msa0930550-mRNA-1:five_prime_utr;Parent=Msa0930550-mRNA-1
chr6_2 five_prime_UTR 79650607 79650658 79650607 ID=Msa0930550-mRNA-1:five_prime_utr;Parent=Msa0930550-mRNA-1
chr6_2 CDS 79650659 79650663 79650659 ID=Msa0930550-mRNA-1:cds;Parent=Msa0930550-mRNA-1
chr6_2 CDS 79650774 79650792 79650774 ID=Msa0930550-mRNA-1:cds;Parent=Msa0930550-mRNA-1
chr6_2 CDS 79651445 79651525 79651445 ID=Msa0930550-mRNA-1:cds;Parent=Msa0930550-mRNA-1
chr6_2 CDS 79651621 79651725 79651621 ID=Msa0930550-mRNA-1:cds;Parent=Msa0930550-mRNA-1
chr6_2 CDS 79651828 79651944 79651828 ID=Msa0930550-mRNA-1:cds;Parent=Msa0930550-mRNA-1
chr6_2 three_prime_UTR 79651945 79652297 79651945 ID=Msa0930550-mRNA-1:three_prime_utr;Parent=Msa0930550-mRNA-1
Gene Sequence

>Msa0930550

ATGAGCCGGCCAATGGAAGAAGATGCTGTCGGGAAGAATGAGGAAGAGGAATTCAACACTGGTCCACTATCTGTACTCATGATGAGTGTGAAAAACAACACACAGGTTCTGATCAATTGTCGTAACAACAAAAAGCTTCTAGGCCGTGTCAGGGCTTTTGATAGACACTGCAATATGGTTCTTGAAAATGTCCGGGAAATGTGGACTGAGGTGCCTAAGACTGGTAAAGGAAAGAAGAAGGCTCAGCCAGTCAACAAGGATCGTTTCATCAGCAAAATGTTCCTTCGTGGAGATTCTGTCATCATTGTTCTTAGGAACCCCAAATAA
Protein sequence

>Msa0930550

MSRPMEEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKAQPVNKDRFISKMFLRGDSVIIVLRNPK*