AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0935130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0935130 MtrunA17_Chr7g0232921 63.830 94 34 0 9 102 108 201 2.81e-41 137
Msa0935130 MtrunA17_Chr8g0366201 56.818 88 37 1 67 154 2 88 7.12e-31 107
Msa0935130 MtrunA17_Chr4g0023021 50.575 87 43 0 68 154 5 91 1.57e-29 104
Msa0935130 MtrunA17_Chr8g0365871 56.757 74 32 0 68 141 14 87 1.04e-28 102
Msa0935130 MtrunA17_Chr6g0473651 36.752 117 62 3 49 158 73 184 5.32e-17 77.0
Msa0935130 MtrunA17_Chr6g0473921 31.579 114 72 2 48 160 2 110 5.27e-15 68.2
Msa0935130 MtrunA17_Chr6g0473661 33.929 112 68 2 54 164 369 475 1.57e-13 67.4
Msa0935130 MtrunA17_Chr1g0156191 33.333 99 65 1 44 141 31 129 2.34e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAATCTCATTGTGATT+TGG 0.281584 6_3:+5586861 None:intergenic
AGAAATTCATTCATTCGATA+TGG 0.286122 6_3:-5586773 Msa0935130:CDS
AAATCATGATTGACTGAAAA+AGG 0.286482 6_3:-5586157 Msa0935130:three_prime_UTR
ATGTACTAAAGTCTCCATTT+TGG 0.291471 6_3:-5585868 Msa0935130:three_prime_UTR
TAACAACATTCACATCTATA+TGG 0.294880 6_3:+5586090 None:intergenic
CACAAAATTCTCCCATCTTC+TGG 0.298930 6_3:-5586729 Msa0935130:CDS
TTCAAGGGTATTTGGTTTAG+AGG 0.311406 6_3:-5586907 Msa0935130:five_prime_UTR
TCCAGCCACATTAGCACATT+TGG 0.336574 6_3:+5586046 None:intergenic
ATTATAAGATTGGGTGTTAC+CGG 0.337980 6_3:-5586342 Msa0935130:CDS
GAAAACTGACATAACCAAAA+TGG 0.347743 6_3:+5585854 None:intergenic
GAAATCCATGAGGACCTTCC+AGG 0.356558 6_3:+5586446 None:intergenic
TTATTTGACTTTAGAGTGTA+TGG 0.358332 6_3:-5586274 Msa0935130:CDS
TACACCAGTGGAAGTTAATA+TGG 0.384671 6_3:+5586023 None:intergenic
CAATGTACTTACCAAGCTTC+TGG 0.388188 6_3:+5586676 None:intergenic
TAAAGGACAAATTATAAGAT+TGG 0.391020 6_3:-5586352 Msa0935130:CDS
AGGTTGTGTTACAGAGTAGA+AGG 0.405838 6_3:-5586137 Msa0935130:three_prime_UTR
CAAAGAAAATTCCATGATTA+CGG 0.407492 6_3:+5585917 None:intergenic
TCCTCAGCAGAAATTGTTGC+TGG 0.408451 6_3:-5586819 Msa0935130:CDS
TGGCTTCAAGTGAGAGTGCC+AGG 0.424618 6_3:-5585827 Msa0935130:three_prime_UTR
TCATTGTTGTTGATTACCTT+GGG 0.426570 6_3:-5586473 Msa0935130:CDS
CTATGGTCAGTGGCAAGCTC+TGG 0.427646 6_3:-5586394 Msa0935130:CDS
GATCCAAATCACAATGAGAT+TGG 0.430194 6_3:-5586864 Msa0935130:CDS
GCTCTTGACAGCAGCAACTA+AGG 0.430331 6_3:+5587147 None:intergenic
GAATGAATTTCTGTAGTCAT+AGG 0.439784 6_3:+5586784 None:intergenic
ATGAATTCAGTGCGCTCTGC+AGG 0.440156 6_3:+5586492 None:intergenic
CACTCTCACTTGAAGCCAAA+AGG 0.447023 6_3:+5585832 None:intergenic
GCGCTTTGTGACCGTAATCA+TGG 0.464370 6_3:-5585928 Msa0935130:three_prime_UTR
AAAGGACAAATTATAAGATT+GGG 0.468907 6_3:-5586351 Msa0935130:CDS
TTTGGATCATGTATTACAAA+AGG 0.483026 6_3:+5586879 None:intergenic
TCCAAATGTGCTAATGTGGC+TGG 0.483967 6_3:-5586047 Msa0935130:three_prime_UTR
TTCATTGTTGTTGATTACCT+TGG 0.487612 6_3:-5586474 Msa0935130:CDS
ACTCTAAAGTCAAATAATGC+AGG 0.487745 6_3:+5586280 None:intergenic
TTCTGCTGAGGATTGAATTG+AGG 0.488055 6_3:+5586830 None:intergenic
GTGCAGAAGCTGGGATGGCT+TGG 0.489900 6_3:-5586534 Msa0935130:intron
AACATCGTTGTTCGATTCAC+CGG 0.492245 6_3:+5586323 None:intergenic
TTAACTCCTTCACCAGAAGA+TGG 0.498394 6_3:+5586717 None:intergenic
TTGGTGTACGCATGTTTGCA+CGG 0.506355 6_3:+5585890 None:intergenic
AGACATTGGTAAGGAGATCC+TGG 0.508977 6_3:+5585809 None:intergenic
TTGATTACCTTGGGTACACC+TGG 0.531266 6_3:-5586464 Msa0935130:CDS
TCCAGCAACAATTTCTGCTG+AGG 0.539383 6_3:+5586818 None:intergenic
GTACACCTGGAAGGTCCTCA+TGG 0.545748 6_3:-5586451 Msa0935130:CDS
TGGACCATATTAACTTCCAC+TGG 0.549812 6_3:-5586027 Msa0935130:three_prime_UTR
AAGGTTAACGATACATATCA+TGG 0.550752 6_3:+5585767 None:intergenic
TGATGTTACTACCAGAAGCT+TGG 0.565250 6_3:-5586687 Msa0935130:intron
TGGTGTACGCATGTTTGCAC+GGG 0.568837 6_3:+5585891 None:intergenic
ATTCTCCCATCTTCTGGTGA+AGG 0.571056 6_3:-5586723 Msa0935130:CDS
CTCTTGACAGCAGCAACTAA+GGG 0.571127 6_3:+5587148 None:intergenic
CTCTAAAGTCAAATAATGCA+GGG 0.574657 6_3:+5586281 None:intergenic
CTCAGTTGATAATAAGACAT+TGG 0.578860 6_3:+5585795 None:intergenic
TAACTCCTTCACCAGAAGAT+GGG 0.579136 6_3:+5586718 None:intergenic
TGTGTGTGCAGAAGCTGGGA+TGG 0.583314 6_3:-5586539 Msa0935130:intron
TTACCTTGGGTACACCTGGA+AGG 0.591641 6_3:-5586460 Msa0935130:CDS
AGTGCACGCAGGTTGACTAA+AGG 0.592704 6_3:-5586369 Msa0935130:CDS
TGTATGTGTGTGCAGAAGCT+GGG 0.593472 6_3:-5586543 Msa0935130:intron
AAATGGAGACTTTAGTACAT+TGG 0.596447 6_3:+5585871 None:intergenic
TTGATAATAAGACATTGGTA+AGG 0.600708 6_3:+5585800 None:intergenic
ATGGATTTCTGTCATGATGA+AGG 0.601737 6_3:-5586432 Msa0935130:CDS
AATACCCTTGAAAAGCATGC+AGG 0.608287 6_3:+5586918 None:intergenic
AACTGTCGAAATTACACCAG+TGG 0.610725 6_3:+5586011 None:intergenic
TCTCTCCAAATGTGCTAATG+TGG 0.628942 6_3:-5586051 Msa0935130:three_prime_UTR
GTCTTATTATCAACTGAGAG+TGG 0.651419 6_3:-5585790 Msa0935130:three_prime_UTR
GGACCTTCCAGGTGTACCCA+AGG 0.652405 6_3:+5586457 None:intergenic
AAGCTCTGGTGAGTGCACGC+AGG 0.677308 6_3:-5586380 Msa0935130:CDS
TTAACAAAGTACATACCACA+TGG 0.700103 6_3:-5587117 Msa0935130:five_prime_UTR
TGCATGAATCATATTCCATG+TGG 0.712298 6_3:+5587102 None:intergenic
TCTGGTGAAGGAGTTAACAG+AGG 0.715932 6_3:-5586711 Msa0935130:CDS
TGCTGCTGTCAAGAGCGATG+TGG 0.743908 6_3:-5587140 Msa0935130:five_prime_UTR
CATCATGACAGAAATCCATG+AGG 0.770644 6_3:+5586436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATATGATCTTTAAA+TGG + chr6_3:5586310-5586329 None:intergenic 10.0%
!! AATTATATATGATCTTTAAA+TGG + chr6_3:5586310-5586329 None:intergenic 10.0%
!! CATACAGAATGAAAATTAAA+AGG + chr6_3:5586369-5586388 None:intergenic 20.0%
!! TAAAGGACAAATTATAAGAT+TGG - chr6_3:5586574-5586593 Msa0935130:intron 20.0%
!! AAAGGACAAATTATAAGATT+GGG - chr6_3:5586575-5586594 Msa0935130:intron 20.0%
!!! CATAAGATTTTTTTTGACAA+TGG - chr6_3:5586687-5586706 Msa0935130:intron 20.0%
!!! CTTTTTTTAAAGATGCAAAA+AGG - chr6_3:5586734-5586753 Msa0935130:CDS 20.0%
!! CATACAGAATGAAAATTAAA+AGG + chr6_3:5586369-5586388 None:intergenic 20.0%
!! TAAAGGACAAATTATAAGAT+TGG - chr6_3:5586574-5586593 Msa0935130:intron 20.0%
!! AAAGGACAAATTATAAGATT+GGG - chr6_3:5586575-5586594 Msa0935130:intron 20.0%
!!! CATAAGATTTTTTTTGACAA+TGG - chr6_3:5586687-5586706 Msa0935130:intron 20.0%
!!! CTTTTTTTAAAGATGCAAAA+AGG - chr6_3:5586734-5586753 Msa0935130:CDS 20.0%
!!! CTATTGTCATTTTAGAAGAA+GGG - chr6_3:5585978-5585997 Msa0935130:three_prime_UTR 25.0%
! TTTGGATCATGTATTACAAA+AGG + chr6_3:5586050-5586069 None:intergenic 25.0%
! AGAAATTCATTCATTCGATA+TGG - chr6_3:5586153-5586172 Msa0935130:three_prime_UTR 25.0%
!! TTTTGTTCATTTTGCATCTT+AGG - chr6_3:5586329-5586348 Msa0935130:CDS 25.0%
!! TTTGTTCATTTTGCATCTTA+GGG - chr6_3:5586330-5586349 Msa0935130:CDS 25.0%
!!! TTATTTGACTTTAGAGTGTA+TGG - chr6_3:5586652-5586671 Msa0935130:intron 25.0%
! AAATCATGATTGACTGAAAA+AGG - chr6_3:5586769-5586788 Msa0935130:CDS 25.0%
! TAACAACATTCACATCTATA+TGG + chr6_3:5586839-5586858 None:intergenic 25.0%
! CAAAGAAAATTCCATGATTA+CGG + chr6_3:5587012-5587031 None:intergenic 25.0%
!! TTGATAATAAGACATTGGTA+AGG + chr6_3:5587129-5587148 None:intergenic 25.0%
!!! CTATTGTCATTTTAGAAGAA+GGG - chr6_3:5585978-5585997 Msa0935130:three_prime_UTR 25.0%
! TTTGGATCATGTATTACAAA+AGG + chr6_3:5586050-5586069 None:intergenic 25.0%
! AGAAATTCATTCATTCGATA+TGG - chr6_3:5586153-5586172 Msa0935130:three_prime_UTR 25.0%
!! TTTTGTTCATTTTGCATCTT+AGG - chr6_3:5586329-5586348 Msa0935130:CDS 25.0%
!! TTTGTTCATTTTGCATCTTA+GGG - chr6_3:5586330-5586349 Msa0935130:CDS 25.0%
!!! TTATTTGACTTTAGAGTGTA+TGG - chr6_3:5586652-5586671 Msa0935130:intron 25.0%
! AAATCATGATTGACTGAAAA+AGG - chr6_3:5586769-5586788 Msa0935130:CDS 25.0%
! TAACAACATTCACATCTATA+TGG + chr6_3:5586839-5586858 None:intergenic 25.0%
! CAAAGAAAATTCCATGATTA+CGG + chr6_3:5587012-5587031 None:intergenic 25.0%
!! TTGATAATAAGACATTGGTA+AGG + chr6_3:5587129-5587148 None:intergenic 25.0%
TTAACAAAGTACATACCACA+TGG - chr6_3:5585809-5585828 Msa0935130:three_prime_UTR 30.0%
ATTAACATTAGACGAATGTC+AGG + chr6_3:5585931-5585950 None:intergenic 30.0%
!!! GCTATTGTCATTTTAGAAGA+AGG - chr6_3:5585977-5585996 Msa0935130:three_prime_UTR 30.0%
GAATGAATTTCTGTAGTCAT+AGG + chr6_3:5586145-5586164 None:intergenic 30.0%
TACATTGTATTTGATGCTCA+TGG - chr6_3:5586266-5586285 Msa0935130:CDS 30.0%
! TTCATTGTTGTTGATTACCT+TGG - chr6_3:5586452-5586471 Msa0935130:CDS 30.0%
! TCATTGTTGTTGATTACCTT+GGG - chr6_3:5586453-5586472 Msa0935130:CDS 30.0%
! ATTATAAGATTGGGTGTTAC+CGG - chr6_3:5586584-5586603 Msa0935130:intron 30.0%
!! CTCTAAAGTCAAATAATGCA+GGG + chr6_3:5586648-5586667 None:intergenic 30.0%
!! ACTCTAAAGTCAAATAATGC+AGG + chr6_3:5586649-5586668 None:intergenic 30.0%
TTAAAGATGCAAAAAGGTGA+TGG - chr6_3:5586740-5586759 Msa0935130:CDS 30.0%
AAATGGAGACTTTAGTACAT+TGG + chr6_3:5587058-5587077 None:intergenic 30.0%
! ATGTACTAAAGTCTCCATTT+TGG - chr6_3:5587058-5587077 Msa0935130:intron 30.0%
GAAAACTGACATAACCAAAA+TGG + chr6_3:5587075-5587094 None:intergenic 30.0%
!! CTCAGTTGATAATAAGACAT+TGG + chr6_3:5587134-5587153 None:intergenic 30.0%
TTAACAAAGTACATACCACA+TGG - chr6_3:5585809-5585828 Msa0935130:three_prime_UTR 30.0%
ATTAACATTAGACGAATGTC+AGG + chr6_3:5585931-5585950 None:intergenic 30.0%
!!! GCTATTGTCATTTTAGAAGA+AGG - chr6_3:5585977-5585996 Msa0935130:three_prime_UTR 30.0%
GAATGAATTTCTGTAGTCAT+AGG + chr6_3:5586145-5586164 None:intergenic 30.0%
TACATTGTATTTGATGCTCA+TGG - chr6_3:5586266-5586285 Msa0935130:CDS 30.0%
! TTCATTGTTGTTGATTACCT+TGG - chr6_3:5586452-5586471 Msa0935130:CDS 30.0%
! TCATTGTTGTTGATTACCTT+GGG - chr6_3:5586453-5586472 Msa0935130:CDS 30.0%
! ATTATAAGATTGGGTGTTAC+CGG - chr6_3:5586584-5586603 Msa0935130:intron 30.0%
!! CTCTAAAGTCAAATAATGCA+GGG + chr6_3:5586648-5586667 None:intergenic 30.0%
!! ACTCTAAAGTCAAATAATGC+AGG + chr6_3:5586649-5586668 None:intergenic 30.0%
TTAAAGATGCAAAAAGGTGA+TGG - chr6_3:5586740-5586759 Msa0935130:CDS 30.0%
AAATGGAGACTTTAGTACAT+TGG + chr6_3:5587058-5587077 None:intergenic 30.0%
! ATGTACTAAAGTCTCCATTT+TGG - chr6_3:5587058-5587077 Msa0935130:intron 30.0%
GAAAACTGACATAACCAAAA+TGG + chr6_3:5587075-5587094 None:intergenic 30.0%
!! CTCAGTTGATAATAAGACAT+TGG + chr6_3:5587134-5587153 None:intergenic 30.0%
TGCATGAATCATATTCCATG+TGG + chr6_3:5585827-5585846 None:intergenic 35.0%
!! TTCAAGGGTATTTGGTTTAG+AGG - chr6_3:5586019-5586038 Msa0935130:three_prime_UTR 35.0%
GATCCAAATCACAATGAGAT+TGG - chr6_3:5586062-5586081 Msa0935130:three_prime_UTR 35.0%
TCTCCAATCTCATTGTGATT+TGG + chr6_3:5586068-5586087 None:intergenic 35.0%
! AGAAGATGGGAGAATTTTGT+GGG + chr6_3:5586198-5586217 None:intergenic 35.0%
ATGGATTTCTGTCATGATGA+AGG - chr6_3:5586494-5586513 Msa0935130:CDS 35.0%
! GGTGTTTCTTGCATTTTCTA+TGG - chr6_3:5586515-5586534 Msa0935130:CDS 35.0%
TACACCAGTGGAAGTTAATA+TGG + chr6_3:5586906-5586925 None:intergenic 35.0%
! GGTTATGTCAGTTTTCCTTT+TGG - chr6_3:5587079-5587098 Msa0935130:intron 35.0%
! GTCTTATTATCAACTGAGAG+TGG - chr6_3:5587136-5587155 Msa0935130:five_prime_UTR 35.0%
TGCATGAATCATATTCCATG+TGG + chr6_3:5585827-5585846 None:intergenic 35.0%
!! TTCAAGGGTATTTGGTTTAG+AGG - chr6_3:5586019-5586038 Msa0935130:three_prime_UTR 35.0%
GATCCAAATCACAATGAGAT+TGG - chr6_3:5586062-5586081 Msa0935130:three_prime_UTR 35.0%
TCTCCAATCTCATTGTGATT+TGG + chr6_3:5586068-5586087 None:intergenic 35.0%
! AGAAGATGGGAGAATTTTGT+GGG + chr6_3:5586198-5586217 None:intergenic 35.0%
ATGGATTTCTGTCATGATGA+AGG - chr6_3:5586494-5586513 Msa0935130:CDS 35.0%
! GGTGTTTCTTGCATTTTCTA+TGG - chr6_3:5586515-5586534 Msa0935130:CDS 35.0%
TACACCAGTGGAAGTTAATA+TGG + chr6_3:5586906-5586925 None:intergenic 35.0%
! GGTTATGTCAGTTTTCCTTT+TGG - chr6_3:5587079-5587098 Msa0935130:intron 35.0%
! GTCTTATTATCAACTGAGAG+TGG - chr6_3:5587136-5587155 Msa0935130:five_prime_UTR 35.0%
! ACTTCCTGCATGCTTTTCAA+GGG - chr6_3:5586004-5586023 Msa0935130:three_prime_UTR 40.0%
! AATACCCTTGAAAAGCATGC+AGG + chr6_3:5586011-5586030 None:intergenic 40.0%
!!! GCATGCTTTTCAAGGGTATT+TGG - chr6_3:5586011-5586030 Msa0935130:three_prime_UTR 40.0%
TTCTGCTGAGGATTGAATTG+AGG + chr6_3:5586099-5586118 None:intergenic 40.0%
! GAAGATGGGAGAATTTTGTG+GGG + chr6_3:5586197-5586216 None:intergenic 40.0%
! CAGAAGATGGGAGAATTTTG+TGG + chr6_3:5586199-5586218 None:intergenic 40.0%
CACAAAATTCTCCCATCTTC+TGG - chr6_3:5586197-5586216 Msa0935130:three_prime_UTR 40.0%
TAACTCCTTCACCAGAAGAT+GGG + chr6_3:5586211-5586230 None:intergenic 40.0%
TTAACTCCTTCACCAGAAGA+TGG + chr6_3:5586212-5586231 None:intergenic 40.0%
TGATGTTACTACCAGAAGCT+TGG - chr6_3:5586239-5586258 Msa0935130:exon 40.0%
CAATGTACTTACCAAGCTTC+TGG + chr6_3:5586253-5586272 None:intergenic 40.0%
CATCATGACAGAAATCCATG+AGG + chr6_3:5586493-5586512 None:intergenic 40.0%
! CTTGCATTTTCTATGGTCAG+TGG - chr6_3:5586522-5586541 Msa0935130:CDS 40.0%
AACATCGTTGTTCGATTCAC+CGG + chr6_3:5586606-5586625 None:intergenic 40.0%
AGGTTGTGTTACAGAGTAGA+AGG - chr6_3:5586789-5586808 Msa0935130:CDS 40.0%
TCTCTCCAAATGTGCTAATG+TGG - chr6_3:5586875-5586894 Msa0935130:exon 40.0%
TGGACCATATTAACTTCCAC+TGG - chr6_3:5586899-5586918 Msa0935130:five_prime_UTR 40.0%
AACTGTCGAAATTACACCAG+TGG + chr6_3:5586918-5586937 None:intergenic 40.0%
! ACTTCCTGCATGCTTTTCAA+GGG - chr6_3:5586004-5586023 Msa0935130:three_prime_UTR 40.0%
! AATACCCTTGAAAAGCATGC+AGG + chr6_3:5586011-5586030 None:intergenic 40.0%
!!! GCATGCTTTTCAAGGGTATT+TGG - chr6_3:5586011-5586030 Msa0935130:three_prime_UTR 40.0%
TTCTGCTGAGGATTGAATTG+AGG + chr6_3:5586099-5586118 None:intergenic 40.0%
! GAAGATGGGAGAATTTTGTG+GGG + chr6_3:5586197-5586216 None:intergenic 40.0%
! CAGAAGATGGGAGAATTTTG+TGG + chr6_3:5586199-5586218 None:intergenic 40.0%
CACAAAATTCTCCCATCTTC+TGG - chr6_3:5586197-5586216 Msa0935130:three_prime_UTR 40.0%
TAACTCCTTCACCAGAAGAT+GGG + chr6_3:5586211-5586230 None:intergenic 40.0%
TTAACTCCTTCACCAGAAGA+TGG + chr6_3:5586212-5586231 None:intergenic 40.0%
TGATGTTACTACCAGAAGCT+TGG - chr6_3:5586239-5586258 Msa0935130:exon 40.0%
CAATGTACTTACCAAGCTTC+TGG + chr6_3:5586253-5586272 None:intergenic 40.0%
CATCATGACAGAAATCCATG+AGG + chr6_3:5586493-5586512 None:intergenic 40.0%
! CTTGCATTTTCTATGGTCAG+TGG - chr6_3:5586522-5586541 Msa0935130:CDS 40.0%
AACATCGTTGTTCGATTCAC+CGG + chr6_3:5586606-5586625 None:intergenic 40.0%
AGGTTGTGTTACAGAGTAGA+AGG - chr6_3:5586789-5586808 Msa0935130:CDS 40.0%
TCTCTCCAAATGTGCTAATG+TGG - chr6_3:5586875-5586894 Msa0935130:exon 40.0%
TGGACCATATTAACTTCCAC+TGG - chr6_3:5586899-5586918 Msa0935130:five_prime_UTR 40.0%
AACTGTCGAAATTACACCAG+TGG + chr6_3:5586918-5586937 None:intergenic 40.0%
CTCTTGACAGCAGCAACTAA+GGG + chr6_3:5585781-5585800 None:intergenic 45.0%
! GACTTCCTGCATGCTTTTCA+AGG - chr6_3:5586003-5586022 Msa0935130:three_prime_UTR 45.0%
TCCTCAGCAGAAATTGTTGC+TGG - chr6_3:5586107-5586126 Msa0935130:three_prime_UTR 45.0%
TCCAGCAACAATTTCTGCTG+AGG + chr6_3:5586111-5586130 None:intergenic 45.0%
ATTCTCCCATCTTCTGGTGA+AGG - chr6_3:5586203-5586222 Msa0935130:three_prime_UTR 45.0%
TCTGGTGAAGGAGTTAACAG+AGG - chr6_3:5586215-5586234 Msa0935130:three_prime_UTR 45.0%
TGTATGTGTGTGCAGAAGCT+GGG - chr6_3:5586383-5586402 Msa0935130:CDS 45.0%
TTGATTACCTTGGGTACACC+TGG - chr6_3:5586462-5586481 Msa0935130:CDS 45.0%
TCCAAATGTGCTAATGTGGC+TGG - chr6_3:5586879-5586898 Msa0935130:exon 45.0%
TCCAGCCACATTAGCACATT+TGG + chr6_3:5586883-5586902 None:intergenic 45.0%
TTGGTGTACGCATGTTTGCA+CGG + chr6_3:5587039-5587058 None:intergenic 45.0%
CACTCTCACTTGAAGCCAAA+AGG + chr6_3:5587097-5587116 None:intergenic 45.0%
AGACATTGGTAAGGAGATCC+TGG + chr6_3:5587120-5587139 None:intergenic 45.0%
CTCTTGACAGCAGCAACTAA+GGG + chr6_3:5585781-5585800 None:intergenic 45.0%
! GACTTCCTGCATGCTTTTCA+AGG - chr6_3:5586003-5586022 Msa0935130:three_prime_UTR 45.0%
TCCTCAGCAGAAATTGTTGC+TGG - chr6_3:5586107-5586126 Msa0935130:three_prime_UTR 45.0%
TCCAGCAACAATTTCTGCTG+AGG + chr6_3:5586111-5586130 None:intergenic 45.0%
ATTCTCCCATCTTCTGGTGA+AGG - chr6_3:5586203-5586222 Msa0935130:three_prime_UTR 45.0%
TCTGGTGAAGGAGTTAACAG+AGG - chr6_3:5586215-5586234 Msa0935130:three_prime_UTR 45.0%
TGTATGTGTGTGCAGAAGCT+GGG - chr6_3:5586383-5586402 Msa0935130:CDS 45.0%
TTGATTACCTTGGGTACACC+TGG - chr6_3:5586462-5586481 Msa0935130:CDS 45.0%
TCCAAATGTGCTAATGTGGC+TGG - chr6_3:5586879-5586898 Msa0935130:exon 45.0%
TCCAGCCACATTAGCACATT+TGG + chr6_3:5586883-5586902 None:intergenic 45.0%
TTGGTGTACGCATGTTTGCA+CGG + chr6_3:5587039-5587058 None:intergenic 45.0%
CACTCTCACTTGAAGCCAAA+AGG + chr6_3:5587097-5587116 None:intergenic 45.0%
AGACATTGGTAAGGAGATCC+TGG + chr6_3:5587120-5587139 None:intergenic 45.0%
GCTCTTGACAGCAGCAACTA+AGG + chr6_3:5585782-5585801 None:intergenic 50.0%
CTGTATGTGTGTGCAGAAGC+TGG - chr6_3:5586382-5586401 Msa0935130:CDS 50.0%
ATGAATTCAGTGCGCTCTGC+AGG + chr6_3:5586437-5586456 None:intergenic 50.0%
TTACCTTGGGTACACCTGGA+AGG - chr6_3:5586466-5586485 Msa0935130:CDS 50.0%
GAAATCCATGAGGACCTTCC+AGG + chr6_3:5586483-5586502 None:intergenic 50.0%
! AGTGCACGCAGGTTGACTAA+AGG - chr6_3:5586557-5586576 Msa0935130:intron 50.0%
GCGCTTTGTGACCGTAATCA+TGG - chr6_3:5586998-5587017 Msa0935130:intron 50.0%
TGGTGTACGCATGTTTGCAC+GGG + chr6_3:5587038-5587057 None:intergenic 50.0%
GCTCTTGACAGCAGCAACTA+AGG + chr6_3:5585782-5585801 None:intergenic 50.0%
CTGTATGTGTGTGCAGAAGC+TGG - chr6_3:5586382-5586401 Msa0935130:CDS 50.0%
ATGAATTCAGTGCGCTCTGC+AGG + chr6_3:5586437-5586456 None:intergenic 50.0%
TTACCTTGGGTACACCTGGA+AGG - chr6_3:5586466-5586485 Msa0935130:CDS 50.0%
GAAATCCATGAGGACCTTCC+AGG + chr6_3:5586483-5586502 None:intergenic 50.0%
! AGTGCACGCAGGTTGACTAA+AGG - chr6_3:5586557-5586576 Msa0935130:intron 50.0%
GCGCTTTGTGACCGTAATCA+TGG - chr6_3:5586998-5587017 Msa0935130:intron 50.0%
TGGTGTACGCATGTTTGCAC+GGG + chr6_3:5587038-5587057 None:intergenic 50.0%
TGCTGCTGTCAAGAGCGATG+TGG - chr6_3:5585786-5585805 Msa0935130:three_prime_UTR 55.0%
TGTGTGTGCAGAAGCTGGGA+TGG - chr6_3:5586387-5586406 Msa0935130:CDS 55.0%
GTACACCTGGAAGGTCCTCA+TGG - chr6_3:5586475-5586494 Msa0935130:CDS 55.0%
CTATGGTCAGTGGCAAGCTC+TGG - chr6_3:5586532-5586551 Msa0935130:intron 55.0%
!! TGGCTTCAAGTGAGAGTGCC+AGG - chr6_3:5587099-5587118 Msa0935130:intron 55.0%
TGCTGCTGTCAAGAGCGATG+TGG - chr6_3:5585786-5585805 Msa0935130:three_prime_UTR 55.0%
TGTGTGTGCAGAAGCTGGGA+TGG - chr6_3:5586387-5586406 Msa0935130:CDS 55.0%
GTACACCTGGAAGGTCCTCA+TGG - chr6_3:5586475-5586494 Msa0935130:CDS 55.0%
CTATGGTCAGTGGCAAGCTC+TGG - chr6_3:5586532-5586551 Msa0935130:intron 55.0%
!! TGGCTTCAAGTGAGAGTGCC+AGG - chr6_3:5587099-5587118 Msa0935130:intron 55.0%
GTGCAGAAGCTGGGATGGCT+TGG - chr6_3:5586392-5586411 Msa0935130:CDS 60.0%
GGACCTTCCAGGTGTACCCA+AGG + chr6_3:5586472-5586491 None:intergenic 60.0%
! AAGCTCTGGTGAGTGCACGC+AGG - chr6_3:5586546-5586565 Msa0935130:intron 60.0%
GTGCAGAAGCTGGGATGGCT+TGG - chr6_3:5586392-5586411 Msa0935130:CDS 60.0%
GGACCTTCCAGGTGTACCCA+AGG + chr6_3:5586472-5586491 None:intergenic 60.0%
! AAGCTCTGGTGAGTGCACGC+AGG - chr6_3:5586546-5586565 Msa0935130:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 5585773 5587175 5585773 ID=Msa0935130;Name=Msa0935130
chr6_3 mRNA 5585773 5587175 5585773 ID=Msa0935130-mRNA-1;Parent=Msa0935130;Name=Msa0935130-mRNA-1;_AED=0.00;_eAED=0.00;_QI=143|1|1|1|0.5|0.33|3|484|164
chr6_3 exon 5585773 5586550 5585773 ID=Msa0935130-mRNA-1:exon:1684;Parent=Msa0935130-mRNA-1
chr6_3 exon 5586688 5586955 5586688 ID=Msa0935130-mRNA-1:exon:1683;Parent=Msa0935130-mRNA-1
chr6_3 exon 5587100 5587175 5587100 ID=Msa0935130-mRNA-1:exon:1682;Parent=Msa0935130-mRNA-1
chr6_3 five_prime_UTR 5587100 5587175 5587100 ID=Msa0935130-mRNA-1:five_prime_utr;Parent=Msa0935130-mRNA-1
chr6_3 five_prime_UTR 5586889 5586955 5586889 ID=Msa0935130-mRNA-1:five_prime_utr;Parent=Msa0935130-mRNA-1
chr6_3 CDS 5586688 5586888 5586688 ID=Msa0935130-mRNA-1:cds;Parent=Msa0935130-mRNA-1
chr6_3 CDS 5586257 5586550 5586257 ID=Msa0935130-mRNA-1:cds;Parent=Msa0935130-mRNA-1
chr6_3 three_prime_UTR 5585773 5586256 5585773 ID=Msa0935130-mRNA-1:three_prime_utr;Parent=Msa0935130-mRNA-1
Gene Sequence

>Msa0935130

ATGATCCAAATCACAATGAGATTGGAGAAGAGTTATCCTCAATTCAATCCTCAGCAGAAATTGTTGCTGGAAAATCAGAGAGCCTATGACTACAGAAATTCATTCATTCGATATGGATCAAATGCTCATTTTCTCCCCACAAAATTCTCCCATCTTCTGGTGAAGGAGTTAACAGAGGCTGATGTTACTACCAGAAGCTTGAAGCTGGGATGGCTTGGATTTTATCAAGCTGTTTTTCCTGCAGAGCGCACTGAATTCATTGTTGTTGATTACCTTGGGTACACCTGGAAGGTCCTCATGGATTTCTGTCATGATGAAGGTGTTTCTTGCATTTTCTATGGTCAGTGGCAAGCTCTGGTGAGTGCACGCAGGTTGACTAAAGGACAAATTATAAGATTGGGTGTTACCGGTGAATCGAACAACGATGTTAAGTATCTTTGCGCAAGAACCCTGCATTATTTGACTTTAGAGTGTATGGAGAATTTATATTCATAA
Protein sequence

>Msa0935130

MIQITMRLEKSYPQFNPQQKLLLENQRAYDYRNSFIRYGSNAHFLPTKFSHLLVKELTEADVTTRSLKLGWLGFYQAVFPAERTEFIVVDYLGYTWKVLMDFCHDEGVSCIFYGQWQALVSARRLTKGQIIRLGVTGESNNDVKYLCARTLHYLTLECMENLYS*