AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1358100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1358100 MtrunA17_Chr7g0232921 63.830 94 34 0 9 102 108 201 2.81e-41 137
Msa1358100 MtrunA17_Chr8g0366201 56.818 88 37 1 67 154 2 88 7.12e-31 107
Msa1358100 MtrunA17_Chr4g0023021 50.575 87 43 0 68 154 5 91 1.57e-29 104
Msa1358100 MtrunA17_Chr8g0365871 56.757 74 32 0 68 141 14 87 1.04e-28 102
Msa1358100 MtrunA17_Chr6g0473651 36.752 117 62 3 49 158 73 184 5.32e-17 77.0
Msa1358100 MtrunA17_Chr6g0473921 31.579 114 72 2 48 160 2 110 5.27e-15 68.2
Msa1358100 MtrunA17_Chr6g0473661 33.929 112 68 2 54 164 369 475 1.57e-13 67.4
Msa1358100 MtrunA17_Chr1g0156191 33.333 99 65 1 44 141 31 129 2.34e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCAATCTCATTGTGATT+TGG 0.281584 tig0016220:+287370 None:intergenic
AGAAATTCATTCATTCGATA+TGG 0.286122 tig0016220:-287282 Msa1358100:CDS
AAATCATGATTGACTGAAAA+AGG 0.286482 tig0016220:-286660 Msa1358100:three_prime_UTR
ATGTACTAAAGTCTCCATTT+TGG 0.291471 tig0016220:-286371 Msa1358100:three_prime_UTR
TAACAACATTCACATCTATA+TGG 0.294880 tig0016220:+286593 None:intergenic
CACAAAATTCTCCCATCTTC+TGG 0.298930 tig0016220:-287238 Msa1358100:CDS
TTCAAGGGTATTTGGTTTAG+AGG 0.311406 tig0016220:-287416 Msa1358100:five_prime_UTR
TCCAGCCACATTAGCACATT+TGG 0.336574 tig0016220:+286549 None:intergenic
ATTATAAGATTGGGTGTTAC+CGG 0.337980 tig0016220:-286846 Msa1358100:CDS
GAAAACTGACATAACCAAAA+TGG 0.347743 tig0016220:+286357 None:intergenic
GAAATCCATGAGGACCTTCC+AGG 0.356558 tig0016220:+286950 None:intergenic
TTATTTGACTTTAGAGTGTA+TGG 0.358332 tig0016220:-286778 Msa1358100:CDS
TACACCAGTGGAAGTTAATA+TGG 0.384671 tig0016220:+286526 None:intergenic
CAATGTACTTACCAAGCTTC+TGG 0.388188 tig0016220:+287185 None:intergenic
TAAAGGACAAATTATAAGAT+TGG 0.391020 tig0016220:-286856 Msa1358100:CDS
AGGTTGTGTTGCAGAGTAGA+AGG 0.395233 tig0016220:-286640 Msa1358100:three_prime_UTR
CAAAGAAAATTCCATGATTA+CGG 0.407492 tig0016220:+286420 None:intergenic
TCCTCAGCAGAAATTGTTGC+TGG 0.408451 tig0016220:-287328 Msa1358100:CDS
TGGCTTCAAGTGAGAGTGCC+AGG 0.424618 tig0016220:-286330 Msa1358100:three_prime_UTR
TCATTGTTGTTGATTACCTT+GGG 0.426570 tig0016220:-286977 Msa1358100:CDS
CTATGGTCAGTGGCAAGCTC+TGG 0.427646 tig0016220:-286898 Msa1358100:CDS
GATCCAAATCACAATGAGAT+TGG 0.430194 tig0016220:-287373 Msa1358100:CDS
GCTCTTGACAGCAGCAACTA+AGG 0.430331 tig0016220:+287656 None:intergenic
GAATGAATTTCTGTAGTCAT+AGG 0.439784 tig0016220:+287293 None:intergenic
ATGAATTCAGTGCGCTCTGC+AGG 0.440156 tig0016220:+286996 None:intergenic
CACTCTCACTTGAAGCCAAA+AGG 0.447023 tig0016220:+286335 None:intergenic
GCGCTTTGTGACCGTAATCA+TGG 0.464370 tig0016220:-286431 Msa1358100:three_prime_UTR
AAAGGACAAATTATAAGATT+GGG 0.468907 tig0016220:-286855 Msa1358100:CDS
GTGCAGAAGCTGGGATGGCT+TGG 0.476564 tig0016220:-287038 Msa1358100:intron
TTTGGATCATGTATTACAAA+AGG 0.483026 tig0016220:+287388 None:intergenic
TCCAAATGTGCTAATGTGGC+TGG 0.483967 tig0016220:-286550 Msa1358100:three_prime_UTR
TTCATTGTTGTTGATTACCT+TGG 0.487612 tig0016220:-286978 Msa1358100:CDS
ACTCTAAAGTCAAATAATGC+AGG 0.487745 tig0016220:+286784 None:intergenic
TTCTGCTGAGGATTGAATTG+AGG 0.488055 tig0016220:+287339 None:intergenic
AACATCGTTGTTCGATTCAC+CGG 0.492245 tig0016220:+286827 None:intergenic
TTAACTCCTTCACCAGAAGA+TGG 0.498394 tig0016220:+287226 None:intergenic
TTGGTGTACGCATGTTTGCA+CGG 0.506355 tig0016220:+286393 None:intergenic
AGACATTGGTAAGGAGATCC+TGG 0.508977 tig0016220:+286312 None:intergenic
TTGATTACCTTGGGTACACC+TGG 0.531266 tig0016220:-286968 Msa1358100:CDS
TCCAGCAACAATTTCTGCTG+AGG 0.539383 tig0016220:+287327 None:intergenic
GTACACCTGGAAGGTCCTCA+TGG 0.545748 tig0016220:-286955 Msa1358100:CDS
TGGACCATATTAACTTCCAC+TGG 0.549812 tig0016220:-286530 Msa1358100:three_prime_UTR
AAGGTTAACGATACATATCA+TGG 0.550752 tig0016220:+286270 None:intergenic
ATTGTGTGCAGAAGCTGGGA+TGG 0.553972 tig0016220:-287043 Msa1358100:intron
TGATGTTACTACCAGAAGCT+TGG 0.565250 tig0016220:-287196 Msa1358100:intron
TGGTGTACGCATGTTTGCAC+GGG 0.568837 tig0016220:+286394 None:intergenic
ATTCTCCCATCTTCTGGTGA+AGG 0.571056 tig0016220:-287232 Msa1358100:CDS
CTCTTGACAGCAGCAACTAA+GGG 0.571127 tig0016220:+287657 None:intergenic
CTCTAAAGTCAAATAATGCA+GGG 0.574657 tig0016220:+286785 None:intergenic
CTCAGTTGATAATAAGACAT+TGG 0.578860 tig0016220:+286298 None:intergenic
TAACTCCTTCACCAGAAGAT+GGG 0.579136 tig0016220:+287227 None:intergenic
GGTTATTGTGTGCAGAAGCT+GGG 0.582200 tig0016220:-287047 Msa1358100:intron
TTACCTTGGGTACACCTGGA+AGG 0.591641 tig0016220:-286964 Msa1358100:CDS
AGTGCACGCAGGTTGACTAA+AGG 0.592704 tig0016220:-286873 Msa1358100:CDS
AAATGGAGACTTTAGTACAT+TGG 0.596447 tig0016220:+286374 None:intergenic
TTGATAATAAGACATTGGTA+AGG 0.600708 tig0016220:+286303 None:intergenic
ATGGATTTCTGTCATGATGA+AGG 0.601737 tig0016220:-286936 Msa1358100:CDS
AATACCCTTGAAAAGCATGC+AGG 0.608287 tig0016220:+287427 None:intergenic
AACTGTCGAAATTACACCAG+TGG 0.610725 tig0016220:+286514 None:intergenic
TCTCTCCAAATGTGCTAATG+TGG 0.628942 tig0016220:-286554 Msa1358100:three_prime_UTR
GTCTTATTATCAACTGAGAG+TGG 0.651419 tig0016220:-286293 Msa1358100:three_prime_UTR
GGACCTTCCAGGTGTACCCA+AGG 0.652405 tig0016220:+286961 None:intergenic
AAGCTCTGGTGAGTGCACGC+AGG 0.677308 tig0016220:-286884 Msa1358100:CDS
TTAACAAAGTACATACCACA+TGG 0.700103 tig0016220:-287626 Msa1358100:five_prime_UTR
TGCATGAATCATATTCCATG+TGG 0.712298 tig0016220:+287611 None:intergenic
TCTGGTGAAGGAGTTAACAG+AGG 0.715932 tig0016220:-287220 Msa1358100:CDS
TGCTGCTGTCAAGAGCGATG+TGG 0.743908 tig0016220:-287649 Msa1358100:five_prime_UTR
CATCATGACAGAAATCCATG+AGG 0.770644 tig0016220:+286940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATATGATCTTTAAA+TGG + tig0016220:286813-286832 None:intergenic 10.0%
!! AATTATATATGATCTTTAAA+TGG + tig0016220:286813-286832 None:intergenic 10.0%
!!! ATAAGATTTTTTTTTGACAA+TGG - tig0016220:287196-287215 Msa1358100:intron 15.0%
!!! ATAAGATTTTTTTTTGACAA+TGG - tig0016220:287196-287215 Msa1358100:intron 15.0%
!! CATACAGAATGAAAATTAAA+AGG + tig0016220:286872-286891 None:intergenic 20.0%
!!! CTTTTAATTTTCATTCTGTA+TGG - tig0016220:286870-286889 Msa1358100:CDS 20.0%
!! TAAAGGACAAATTATAAGAT+TGG - tig0016220:287082-287101 Msa1358100:intron 20.0%
!! AAAGGACAAATTATAAGATT+GGG - tig0016220:287083-287102 Msa1358100:intron 20.0%
!!! CTTTTTTTAAAGATGCAAAA+AGG - tig0016220:287243-287262 Msa1358100:CDS 20.0%
!! CATACAGAATGAAAATTAAA+AGG + tig0016220:286872-286891 None:intergenic 20.0%
!!! CTTTTAATTTTCATTCTGTA+TGG - tig0016220:286870-286889 Msa1358100:CDS 20.0%
!! TAAAGGACAAATTATAAGAT+TGG - tig0016220:287082-287101 Msa1358100:intron 20.0%
!! AAAGGACAAATTATAAGATT+GGG - tig0016220:287083-287102 Msa1358100:intron 20.0%
!!! CTTTTTTTAAAGATGCAAAA+AGG - tig0016220:287243-287262 Msa1358100:CDS 20.0%
!!! CTATTGTCATTTTAGAAGAA+GGG - tig0016220:286481-286500 Msa1358100:three_prime_UTR 25.0%
! TTTGGATCATGTATTACAAA+AGG + tig0016220:286553-286572 None:intergenic 25.0%
! AGAAATTCATTCATTCGATA+TGG - tig0016220:286656-286675 Msa1358100:three_prime_UTR 25.0%
!! TTTTGTTCATTTTGCATCTT+AGG - tig0016220:286832-286851 Msa1358100:CDS 25.0%
!! TTTGTTCATTTTGCATCTTA+GGG - tig0016220:286833-286852 Msa1358100:CDS 25.0%
!!! TTATTTGACTTTAGAGTGTA+TGG - tig0016220:287160-287179 Msa1358100:intron 25.0%
! AAATCATGATTGACTGAAAA+AGG - tig0016220:287278-287297 Msa1358100:CDS 25.0%
! TAACAACATTCACATCTATA+TGG + tig0016220:287348-287367 None:intergenic 25.0%
! CAAAGAAAATTCCATGATTA+CGG + tig0016220:287521-287540 None:intergenic 25.0%
!! TTGATAATAAGACATTGGTA+AGG + tig0016220:287638-287657 None:intergenic 25.0%
!!! CTATTGTCATTTTAGAAGAA+GGG - tig0016220:286481-286500 Msa1358100:three_prime_UTR 25.0%
! TTTGGATCATGTATTACAAA+AGG + tig0016220:286553-286572 None:intergenic 25.0%
! AGAAATTCATTCATTCGATA+TGG - tig0016220:286656-286675 Msa1358100:three_prime_UTR 25.0%
!! TTTTGTTCATTTTGCATCTT+AGG - tig0016220:286832-286851 Msa1358100:CDS 25.0%
!! TTTGTTCATTTTGCATCTTA+GGG - tig0016220:286833-286852 Msa1358100:CDS 25.0%
!!! TTATTTGACTTTAGAGTGTA+TGG - tig0016220:287160-287179 Msa1358100:intron 25.0%
! AAATCATGATTGACTGAAAA+AGG - tig0016220:287278-287297 Msa1358100:CDS 25.0%
! TAACAACATTCACATCTATA+TGG + tig0016220:287348-287367 None:intergenic 25.0%
! CAAAGAAAATTCCATGATTA+CGG + tig0016220:287521-287540 None:intergenic 25.0%
!! TTGATAATAAGACATTGGTA+AGG + tig0016220:287638-287657 None:intergenic 25.0%
TTAACAAAGTACATACCACA+TGG - tig0016220:286312-286331 Msa1358100:three_prime_UTR 30.0%
ATTAACATTAGACGAATGTC+AGG + tig0016220:286434-286453 None:intergenic 30.0%
!!! GCTATTGTCATTTTAGAAGA+AGG - tig0016220:286480-286499 Msa1358100:three_prime_UTR 30.0%
GAATGAATTTCTGTAGTCAT+AGG + tig0016220:286648-286667 None:intergenic 30.0%
TACATTGTATTTGATGCTCA+TGG - tig0016220:286769-286788 Msa1358100:CDS 30.0%
! TTCATTGTTGTTGATTACCT+TGG - tig0016220:286960-286979 Msa1358100:CDS 30.0%
! TCATTGTTGTTGATTACCTT+GGG - tig0016220:286961-286980 Msa1358100:CDS 30.0%
! ATTATAAGATTGGGTGTTAC+CGG - tig0016220:287092-287111 Msa1358100:intron 30.0%
!! CTCTAAAGTCAAATAATGCA+GGG + tig0016220:287156-287175 None:intergenic 30.0%
!! ACTCTAAAGTCAAATAATGC+AGG + tig0016220:287157-287176 None:intergenic 30.0%
TTAAAGATGCAAAAAGGTGA+TGG - tig0016220:287249-287268 Msa1358100:CDS 30.0%
AAATGGAGACTTTAGTACAT+TGG + tig0016220:287567-287586 None:intergenic 30.0%
! ATGTACTAAAGTCTCCATTT+TGG - tig0016220:287567-287586 Msa1358100:intron 30.0%
GAAAACTGACATAACCAAAA+TGG + tig0016220:287584-287603 None:intergenic 30.0%
!! CTCAGTTGATAATAAGACAT+TGG + tig0016220:287643-287662 None:intergenic 30.0%
TTAACAAAGTACATACCACA+TGG - tig0016220:286312-286331 Msa1358100:three_prime_UTR 30.0%
ATTAACATTAGACGAATGTC+AGG + tig0016220:286434-286453 None:intergenic 30.0%
!!! GCTATTGTCATTTTAGAAGA+AGG - tig0016220:286480-286499 Msa1358100:three_prime_UTR 30.0%
GAATGAATTTCTGTAGTCAT+AGG + tig0016220:286648-286667 None:intergenic 30.0%
TACATTGTATTTGATGCTCA+TGG - tig0016220:286769-286788 Msa1358100:CDS 30.0%
! TTCATTGTTGTTGATTACCT+TGG - tig0016220:286960-286979 Msa1358100:CDS 30.0%
! TCATTGTTGTTGATTACCTT+GGG - tig0016220:286961-286980 Msa1358100:CDS 30.0%
! ATTATAAGATTGGGTGTTAC+CGG - tig0016220:287092-287111 Msa1358100:intron 30.0%
!! CTCTAAAGTCAAATAATGCA+GGG + tig0016220:287156-287175 None:intergenic 30.0%
!! ACTCTAAAGTCAAATAATGC+AGG + tig0016220:287157-287176 None:intergenic 30.0%
TTAAAGATGCAAAAAGGTGA+TGG - tig0016220:287249-287268 Msa1358100:CDS 30.0%
AAATGGAGACTTTAGTACAT+TGG + tig0016220:287567-287586 None:intergenic 30.0%
! ATGTACTAAAGTCTCCATTT+TGG - tig0016220:287567-287586 Msa1358100:intron 30.0%
GAAAACTGACATAACCAAAA+TGG + tig0016220:287584-287603 None:intergenic 30.0%
!! CTCAGTTGATAATAAGACAT+TGG + tig0016220:287643-287662 None:intergenic 30.0%
TGCATGAATCATATTCCATG+TGG + tig0016220:286330-286349 None:intergenic 35.0%
!! TTCAAGGGTATTTGGTTTAG+AGG - tig0016220:286522-286541 Msa1358100:three_prime_UTR 35.0%
GATCCAAATCACAATGAGAT+TGG - tig0016220:286565-286584 Msa1358100:three_prime_UTR 35.0%
TCTCCAATCTCATTGTGATT+TGG + tig0016220:286571-286590 None:intergenic 35.0%
! AGAAGATGGGAGAATTTTGT+GGG + tig0016220:286701-286720 None:intergenic 35.0%
ATGGATTTCTGTCATGATGA+AGG - tig0016220:287002-287021 Msa1358100:CDS 35.0%
! GGTGTTTCTTGCATTTTCTA+TGG - tig0016220:287023-287042 Msa1358100:CDS 35.0%
TACACCAGTGGAAGTTAATA+TGG + tig0016220:287415-287434 None:intergenic 35.0%
! GGTTATGTCAGTTTTCCTTT+TGG - tig0016220:287588-287607 Msa1358100:intron 35.0%
! GTCTTATTATCAACTGAGAG+TGG - tig0016220:287645-287664 Msa1358100:five_prime_UTR 35.0%
TGCATGAATCATATTCCATG+TGG + tig0016220:286330-286349 None:intergenic 35.0%
!! TTCAAGGGTATTTGGTTTAG+AGG - tig0016220:286522-286541 Msa1358100:three_prime_UTR 35.0%
GATCCAAATCACAATGAGAT+TGG - tig0016220:286565-286584 Msa1358100:three_prime_UTR 35.0%
TCTCCAATCTCATTGTGATT+TGG + tig0016220:286571-286590 None:intergenic 35.0%
! AGAAGATGGGAGAATTTTGT+GGG + tig0016220:286701-286720 None:intergenic 35.0%
ATGGATTTCTGTCATGATGA+AGG - tig0016220:287002-287021 Msa1358100:CDS 35.0%
! GGTGTTTCTTGCATTTTCTA+TGG - tig0016220:287023-287042 Msa1358100:CDS 35.0%
TACACCAGTGGAAGTTAATA+TGG + tig0016220:287415-287434 None:intergenic 35.0%
! GGTTATGTCAGTTTTCCTTT+TGG - tig0016220:287588-287607 Msa1358100:intron 35.0%
! GTCTTATTATCAACTGAGAG+TGG - tig0016220:287645-287664 Msa1358100:five_prime_UTR 35.0%
! ACTTCCTGCATGCTTTTCAA+GGG - tig0016220:286507-286526 Msa1358100:three_prime_UTR 40.0%
! AATACCCTTGAAAAGCATGC+AGG + tig0016220:286514-286533 None:intergenic 40.0%
!!! GCATGCTTTTCAAGGGTATT+TGG - tig0016220:286514-286533 Msa1358100:three_prime_UTR 40.0%
TTCTGCTGAGGATTGAATTG+AGG + tig0016220:286602-286621 None:intergenic 40.0%
! GAAGATGGGAGAATTTTGTG+GGG + tig0016220:286700-286719 None:intergenic 40.0%
! CAGAAGATGGGAGAATTTTG+TGG + tig0016220:286702-286721 None:intergenic 40.0%
CACAAAATTCTCCCATCTTC+TGG - tig0016220:286700-286719 Msa1358100:three_prime_UTR 40.0%
TAACTCCTTCACCAGAAGAT+GGG + tig0016220:286714-286733 None:intergenic 40.0%
TTAACTCCTTCACCAGAAGA+TGG + tig0016220:286715-286734 None:intergenic 40.0%
TGATGTTACTACCAGAAGCT+TGG - tig0016220:286742-286761 Msa1358100:exon 40.0%
CAATGTACTTACCAAGCTTC+TGG + tig0016220:286756-286775 None:intergenic 40.0%
CATCATGACAGAAATCCATG+AGG + tig0016220:287001-287020 None:intergenic 40.0%
! CTTGCATTTTCTATGGTCAG+TGG - tig0016220:287030-287049 Msa1358100:CDS 40.0%
AACATCGTTGTTCGATTCAC+CGG + tig0016220:287114-287133 None:intergenic 40.0%
TCTCTCCAAATGTGCTAATG+TGG - tig0016220:287384-287403 Msa1358100:exon 40.0%
TGGACCATATTAACTTCCAC+TGG - tig0016220:287408-287427 Msa1358100:five_prime_UTR 40.0%
AACTGTCGAAATTACACCAG+TGG + tig0016220:287427-287446 None:intergenic 40.0%
! ACTTCCTGCATGCTTTTCAA+GGG - tig0016220:286507-286526 Msa1358100:three_prime_UTR 40.0%
! AATACCCTTGAAAAGCATGC+AGG + tig0016220:286514-286533 None:intergenic 40.0%
!!! GCATGCTTTTCAAGGGTATT+TGG - tig0016220:286514-286533 Msa1358100:three_prime_UTR 40.0%
TTCTGCTGAGGATTGAATTG+AGG + tig0016220:286602-286621 None:intergenic 40.0%
! GAAGATGGGAGAATTTTGTG+GGG + tig0016220:286700-286719 None:intergenic 40.0%
! CAGAAGATGGGAGAATTTTG+TGG + tig0016220:286702-286721 None:intergenic 40.0%
CACAAAATTCTCCCATCTTC+TGG - tig0016220:286700-286719 Msa1358100:three_prime_UTR 40.0%
TAACTCCTTCACCAGAAGAT+GGG + tig0016220:286714-286733 None:intergenic 40.0%
TTAACTCCTTCACCAGAAGA+TGG + tig0016220:286715-286734 None:intergenic 40.0%
TGATGTTACTACCAGAAGCT+TGG - tig0016220:286742-286761 Msa1358100:exon 40.0%
CAATGTACTTACCAAGCTTC+TGG + tig0016220:286756-286775 None:intergenic 40.0%
CATCATGACAGAAATCCATG+AGG + tig0016220:287001-287020 None:intergenic 40.0%
! CTTGCATTTTCTATGGTCAG+TGG - tig0016220:287030-287049 Msa1358100:CDS 40.0%
AACATCGTTGTTCGATTCAC+CGG + tig0016220:287114-287133 None:intergenic 40.0%
TCTCTCCAAATGTGCTAATG+TGG - tig0016220:287384-287403 Msa1358100:exon 40.0%
TGGACCATATTAACTTCCAC+TGG - tig0016220:287408-287427 Msa1358100:five_prime_UTR 40.0%
AACTGTCGAAATTACACCAG+TGG + tig0016220:287427-287446 None:intergenic 40.0%
CTCTTGACAGCAGCAACTAA+GGG + tig0016220:286284-286303 None:intergenic 45.0%
! GACTTCCTGCATGCTTTTCA+AGG - tig0016220:286506-286525 Msa1358100:three_prime_UTR 45.0%
TCCTCAGCAGAAATTGTTGC+TGG - tig0016220:286610-286629 Msa1358100:three_prime_UTR 45.0%
TCCAGCAACAATTTCTGCTG+AGG + tig0016220:286614-286633 None:intergenic 45.0%
ATTCTCCCATCTTCTGGTGA+AGG - tig0016220:286706-286725 Msa1358100:three_prime_UTR 45.0%
TCTGGTGAAGGAGTTAACAG+AGG - tig0016220:286718-286737 Msa1358100:three_prime_UTR 45.0%
TGGTTATTGTGTGCAGAAGC+TGG - tig0016220:286890-286909 Msa1358100:CDS 45.0%
GGTTATTGTGTGCAGAAGCT+GGG - tig0016220:286891-286910 Msa1358100:CDS 45.0%
TTGATTACCTTGGGTACACC+TGG - tig0016220:286970-286989 Msa1358100:CDS 45.0%
AGGTTGTGTTGCAGAGTAGA+AGG - tig0016220:287298-287317 Msa1358100:CDS 45.0%
TCCAAATGTGCTAATGTGGC+TGG - tig0016220:287388-287407 Msa1358100:exon 45.0%
TCCAGCCACATTAGCACATT+TGG + tig0016220:287392-287411 None:intergenic 45.0%
TTGGTGTACGCATGTTTGCA+CGG + tig0016220:287548-287567 None:intergenic 45.0%
CACTCTCACTTGAAGCCAAA+AGG + tig0016220:287606-287625 None:intergenic 45.0%
AGACATTGGTAAGGAGATCC+TGG + tig0016220:287629-287648 None:intergenic 45.0%
CTCTTGACAGCAGCAACTAA+GGG + tig0016220:286284-286303 None:intergenic 45.0%
! GACTTCCTGCATGCTTTTCA+AGG - tig0016220:286506-286525 Msa1358100:three_prime_UTR 45.0%
TCCTCAGCAGAAATTGTTGC+TGG - tig0016220:286610-286629 Msa1358100:three_prime_UTR 45.0%
TCCAGCAACAATTTCTGCTG+AGG + tig0016220:286614-286633 None:intergenic 45.0%
ATTCTCCCATCTTCTGGTGA+AGG - tig0016220:286706-286725 Msa1358100:three_prime_UTR 45.0%
TCTGGTGAAGGAGTTAACAG+AGG - tig0016220:286718-286737 Msa1358100:three_prime_UTR 45.0%
TGGTTATTGTGTGCAGAAGC+TGG - tig0016220:286890-286909 Msa1358100:CDS 45.0%
GGTTATTGTGTGCAGAAGCT+GGG - tig0016220:286891-286910 Msa1358100:CDS 45.0%
TTGATTACCTTGGGTACACC+TGG - tig0016220:286970-286989 Msa1358100:CDS 45.0%
AGGTTGTGTTGCAGAGTAGA+AGG - tig0016220:287298-287317 Msa1358100:CDS 45.0%
TCCAAATGTGCTAATGTGGC+TGG - tig0016220:287388-287407 Msa1358100:exon 45.0%
TCCAGCCACATTAGCACATT+TGG + tig0016220:287392-287411 None:intergenic 45.0%
TTGGTGTACGCATGTTTGCA+CGG + tig0016220:287548-287567 None:intergenic 45.0%
CACTCTCACTTGAAGCCAAA+AGG + tig0016220:287606-287625 None:intergenic 45.0%
AGACATTGGTAAGGAGATCC+TGG + tig0016220:287629-287648 None:intergenic 45.0%
GCTCTTGACAGCAGCAACTA+AGG + tig0016220:286285-286304 None:intergenic 50.0%
ATTGTGTGCAGAAGCTGGGA+TGG - tig0016220:286895-286914 Msa1358100:CDS 50.0%
ATGAATTCAGTGCGCTCTGC+AGG + tig0016220:286945-286964 None:intergenic 50.0%
TTACCTTGGGTACACCTGGA+AGG - tig0016220:286974-286993 Msa1358100:CDS 50.0%
GAAATCCATGAGGACCTTCC+AGG + tig0016220:286991-287010 None:intergenic 50.0%
! AGTGCACGCAGGTTGACTAA+AGG - tig0016220:287065-287084 Msa1358100:intron 50.0%
GCGCTTTGTGACCGTAATCA+TGG - tig0016220:287507-287526 Msa1358100:intron 50.0%
TGGTGTACGCATGTTTGCAC+GGG + tig0016220:287547-287566 None:intergenic 50.0%
GCTCTTGACAGCAGCAACTA+AGG + tig0016220:286285-286304 None:intergenic 50.0%
ATTGTGTGCAGAAGCTGGGA+TGG - tig0016220:286895-286914 Msa1358100:CDS 50.0%
ATGAATTCAGTGCGCTCTGC+AGG + tig0016220:286945-286964 None:intergenic 50.0%
TTACCTTGGGTACACCTGGA+AGG - tig0016220:286974-286993 Msa1358100:CDS 50.0%
GAAATCCATGAGGACCTTCC+AGG + tig0016220:286991-287010 None:intergenic 50.0%
! AGTGCACGCAGGTTGACTAA+AGG - tig0016220:287065-287084 Msa1358100:intron 50.0%
GCGCTTTGTGACCGTAATCA+TGG - tig0016220:287507-287526 Msa1358100:intron 50.0%
TGGTGTACGCATGTTTGCAC+GGG + tig0016220:287547-287566 None:intergenic 50.0%
TGCTGCTGTCAAGAGCGATG+TGG - tig0016220:286289-286308 Msa1358100:three_prime_UTR 55.0%
GTACACCTGGAAGGTCCTCA+TGG - tig0016220:286983-287002 Msa1358100:CDS 55.0%
CTATGGTCAGTGGCAAGCTC+TGG - tig0016220:287040-287059 Msa1358100:intron 55.0%
!! TGGCTTCAAGTGAGAGTGCC+AGG - tig0016220:287608-287627 Msa1358100:intron 55.0%
TGCTGCTGTCAAGAGCGATG+TGG - tig0016220:286289-286308 Msa1358100:three_prime_UTR 55.0%
GTACACCTGGAAGGTCCTCA+TGG - tig0016220:286983-287002 Msa1358100:CDS 55.0%
CTATGGTCAGTGGCAAGCTC+TGG - tig0016220:287040-287059 Msa1358100:intron 55.0%
!! TGGCTTCAAGTGAGAGTGCC+AGG - tig0016220:287608-287627 Msa1358100:intron 55.0%
GTGCAGAAGCTGGGATGGCT+TGG - tig0016220:286900-286919 Msa1358100:CDS 60.0%
GGACCTTCCAGGTGTACCCA+AGG + tig0016220:286980-286999 None:intergenic 60.0%
! AAGCTCTGGTGAGTGCACGC+AGG - tig0016220:287054-287073 Msa1358100:intron 60.0%
GTGCAGAAGCTGGGATGGCT+TGG - tig0016220:286900-286919 Msa1358100:CDS 60.0%
GGACCTTCCAGGTGTACCCA+AGG + tig0016220:286980-286999 None:intergenic 60.0%
! AAGCTCTGGTGAGTGCACGC+AGG - tig0016220:287054-287073 Msa1358100:intron 60.0%
Chromosome Type Strat End Strand Name
tig0016220 gene 286276 287684 286276 ID=Msa1358100;Name=Msa1358100
tig0016220 mRNA 286276 287684 286276 ID=Msa1358100-mRNA-1;Parent=Msa1358100;Name=Msa1358100-mRNA-1;_AED=0.03;_eAED=0.03;_QI=143|1|1|1|0.5|0.33|3|485|164
tig0016220 exon 286276 287054 286276 ID=Msa1358100-mRNA-1:exon:10642;Parent=Msa1358100-mRNA-1
tig0016220 exon 287197 287464 287197 ID=Msa1358100-mRNA-1:exon:10641;Parent=Msa1358100-mRNA-1
tig0016220 exon 287609 287684 287609 ID=Msa1358100-mRNA-1:exon:10640;Parent=Msa1358100-mRNA-1
tig0016220 five_prime_UTR 287609 287684 287609 ID=Msa1358100-mRNA-1:five_prime_utr;Parent=Msa1358100-mRNA-1
tig0016220 five_prime_UTR 287398 287464 287398 ID=Msa1358100-mRNA-1:five_prime_utr;Parent=Msa1358100-mRNA-1
tig0016220 CDS 287197 287397 287197 ID=Msa1358100-mRNA-1:cds;Parent=Msa1358100-mRNA-1
tig0016220 CDS 286761 287054 286761 ID=Msa1358100-mRNA-1:cds;Parent=Msa1358100-mRNA-1
tig0016220 three_prime_UTR 286276 286760 286276 ID=Msa1358100-mRNA-1:three_prime_utr;Parent=Msa1358100-mRNA-1
Gene Sequence

>Msa1358100

ATGATCCAAATCACAATGAGATTGGAGAAGAGTTATCCTCAATTCAATCCTCAGCAGAAATTGTTGCTGGAAAATCAGAGAGCCTATGACTACAGAAATTCATTCATTCGATATGGATCAAATGCTCATTTTCTCCCCACAAAATTCTCCCATCTTCTGGTGAAGGAGTTAACAGAGGCTGATGTTACTACCAGAAGCTTGAAGCTGGGATGGCTTGGATTTTATCAAGCTGTTTTTCCTGCAGAGCGCACTGAATTCATTGTTGTTGATTACCTTGGGTACACCTGGAAGGTCCTCATGGATTTCTGTCATGATGAAGGTGTTTCTTGCATTTTCTATGGTCAGTGGCAAGCTCTGGTGAGTGCACGCAGGTTGACTAAAGGACAAATTATAAGATTGGGTGTTACCGGTGAATCGAACAACGATGTTAAGTATCTTTGCGCAAGAACCCTGCATTATTTGACTTTAGAGTGTATGGAGAATTTATATTCATAA
Protein sequence

>Msa1358100

MIQITMRLEKSYPQFNPQQKLLLENQRAYDYRNSFIRYGSNAHFLPTKFSHLLVKELTEADVTTRSLKLGWLGFYQAVFPAERTEFIVVDYLGYTWKVLMDFCHDEGVSCIFYGQWQALVSARRLTKGQIIRLGVTGESNNDVKYLCARTLHYLTLECMENLYS*