AlfalfaGEDB | Msa1091500

M. sativa cultivar zhongmu-4 / Msa1091500

KEGG: K02942
GO: GO:0001932 GO:0001934 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006412 GO:0006518 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009893 GO:0009987 GO:0010467 GO:0010562 GO:0010604 GO:0015934 GO:0016020 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019538 GO:0019887 GO:0022625 GO:0022626 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032991 GO:0033674 GO:0034641 GO:0034645 GO:0042325 GO:0042327 GO:0042788 GO:0043043 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080090 GO:0098772 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00428.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1091500	PNX74316.1	92.537	67	5	0	1	67	1	67	2.04e-37	132

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1091500	sp\|P52855\|RLA1_MAIZE	68.254	63	20	0	1	63	1	63	5.16e-26	95.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1091500	A0A2K3L706	92.537	67	5	0	1	67	1	67	9.74e-38	132

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1091500	Msa1091980	0.801178	1.017825e-48	-8.615850e-47
Msa1091500	Msa1358100	0.802215	6.237221e-49	-8.615850e-47
Msa0935130	Msa1091500	0.816340	5.815571e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1091500	MtrunA17_Chr3g0127831	95.082	61	3	0	1	61	1	61	1.23e-38	125
Msa1091500	MtrunA17_Chr2g0311301	95.082	61	3	0	1	61	1	61	1.23e-38	125
Msa1091500	MtrunA17_Chr4g0013621	93.443	61	4	0	1	61	1	61	2.47e-37	121
Msa1091500	MtrunA17_Chr8g0345951	67.606	71	22	1	1	71	1	70	2.84e-28	98.6
Msa1091500	MtrunA17_Chr7g0276131	66.102	59	20	0	1	59	1	59	5.77e-21	80.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1091500	AT5G24510.1	61.628	86	32	1	1	86	1	85	6.52e-31	105
Msa1091500	AT5G47700.1	65.517	58	20	0	4	61	5	62	3.84e-24	88.6
Msa1091500	AT5G47700.2	65.517	58	20	0	4	61	5	62	3.84e-24	88.6
Msa1091500	AT1G01100.3	63.793	58	21	0	4	61	5	62	9.03e-24	87.4
Msa1091500	AT1G01100.4	63.793	58	21	0	4	61	5	62	1.49e-23	87.4
Msa1091500	AT1G01100.1	63.793	58	21	0	4	61	5	62	1.49e-23	87.4
Msa1091500	AT1G01100.2	63.793	58	21	0	4	61	5	62	1.49e-23	87.4
Msa1091500	AT4G00810.1	62.069	58	22	0	4	61	5	62	8.30e-23	85.5
Msa1091500	AT4G00810.2	62.069	58	22	0	4	61	5	62	8.30e-23	85.5

Find 21 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATAATAGACTTACGGTGAT+TGG	0.316212	7_3:+6795524	None:intergenic
TCTGAGCAAGCTTAGCGAAT+AGG	0.386873	7_3:+6795382	None:intergenic
AAAATGAGTTCCGGCGAGTT+GGG	0.395726	7_3:-6795587	Msa1091500:CDS
GCAAGCTTAGCGAATAGGTT+TGG	0.436712	7_3:+6795387	None:intergenic
CAAAATGAGTTCCGGCGAGT+TGG	0.444981	7_3:-6795588	Msa1091500:exon
ATAATAGACTTACGGTGATT+GGG	0.445449	7_3:+6795525	None:intergenic
GAAGATCGGCACTTTGTTGA+AGG	0.446681	7_3:-6795439	Msa1091500:CDS
TTGAACTCCGGCGCTGTTGG+TGG	0.503960	7_3:-6795336	Msa1091500:CDS
ACAATTCACAAAATGAGTTC+CGG	0.522809	7_3:-6795596	Msa1091500:exon
ACGTCACCGTTGAATCCTAC+TGG	0.524586	7_3:-6795410	Msa1091500:CDS
ACCTTGATCCTCCACGACGA+TGG	0.533754	7_3:-6795551	Msa1091500:CDS
TCCATCGTCGTGGAGGATCA+AGG	0.539738	7_3:+6795550	None:intergenic
GGAGGCACCACCAACAGCGC+CGG	0.561097	7_3:+6795329	None:intergenic
AGCGAATAGGTTTGGCCAGT+AGG	0.571609	7_3:+6795395	None:intergenic
GTTTGGCCAGTAGGATTCAA+CGG	0.591956	7_3:+6795404	None:intergenic
AGCATGAGCAAGCTTAGCAA+CGG	0.624729	7_3:+6795308	None:intergenic
GTAAATGCAACCCAACTCGC+CGG	0.634662	7_3:+6795577	None:intergenic
ATGAGCAAGCTTAGCAACGG+AGG	0.638225	7_3:+6795311	None:intergenic
TTGGGATTCCATCGTCGTGG+AGG	0.650788	7_3:+6795543	None:intergenic
TGATTGGGATTCCATCGTCG+TGG	0.662981	7_3:+6795540	None:intergenic
GAACTAACTGAAAAGGAACG+AGG	0.735206	7_3:+6795255	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAAAAAGATAATAGACTTA+CGG	+	chr7_3:6795321-6795340	None:intergenic	20.0%
!!!	TCATAGATCTCTTTTTTTTT+AGG	-	chr7_3:6795369-6795388	Msa1091500:CDS	20.0%
!!!	TAGATCTCTTTTTTTTTAGG+CGG	-	chr7_3:6795372-6795391	Msa1091500:CDS	25.0%
!	TGAAAAAGAACTAACTGAAA+AGG	+	chr7_3:6795590-6795609	None:intergenic	25.0%
	ACAATTCACAAAATGAGTTC+CGG	-	chr7_3:6795239-6795258	Msa1091500:CDS	30.0%
	ATAATAGACTTACGGTGATT+GGG	+	chr7_3:6795313-6795332	None:intergenic	30.0%
	AAAGAGATCTATGAGCAATT+TGG	+	chr7_3:6795363-6795382	None:intergenic	30.0%
	GATAATAGACTTACGGTGAT+TGG	+	chr7_3:6795314-6795333	None:intergenic	35.0%
!!!	TTTTTTTAGGCGGAGAAGAT+CGG	-	chr7_3:6795382-6795401	Msa1091500:CDS	35.0%
!	GATGATCTCATTTTGAACTC+CGG	-	chr7_3:6795487-6795506	Msa1091500:intron	35.0%
	GAACTAACTGAAAAGGAACG+AGG	+	chr7_3:6795583-6795602	None:intergenic	40.0%
	AAAATGAGTTCCGGCGAGTT+GGG	-	chr7_3:6795248-6795267	Msa1091500:CDS	45.0%
	GAAGATCGGCACTTTGTTGA+AGG	-	chr7_3:6795396-6795415	Msa1091500:CDS	45.0%
	GTTTGGCCAGTAGGATTCAA+CGG	+	chr7_3:6795434-6795453	None:intergenic	45.0%
	GCAAGCTTAGCGAATAGGTT+TGG	+	chr7_3:6795451-6795470	None:intergenic	45.0%
	TCTGAGCAAGCTTAGCGAAT+AGG	+	chr7_3:6795456-6795475	None:intergenic	45.0%
	AGCATGAGCAAGCTTAGCAA+CGG	+	chr7_3:6795530-6795549	None:intergenic	45.0%
	CAAAATGAGTTCCGGCGAGT+TGG	-	chr7_3:6795247-6795266	Msa1091500:CDS	50.0%
	GTAAATGCAACCCAACTCGC+CGG	+	chr7_3:6795261-6795280	None:intergenic	50.0%
	TGATTGGGATTCCATCGTCG+TGG	+	chr7_3:6795298-6795317	None:intergenic	50.0%
	ACGTCACCGTTGAATCCTAC+TGG	-	chr7_3:6795425-6795444	Msa1091500:CDS	50.0%
	AGCGAATAGGTTTGGCCAGT+AGG	+	chr7_3:6795443-6795462	None:intergenic	50.0%
!	ATTTTGAACTCCGGCGCTGT+TGG	-	chr7_3:6795496-6795515	Msa1091500:intron	50.0%
	ATGAGCAAGCTTAGCAACGG+AGG	+	chr7_3:6795527-6795546	None:intergenic	50.0%
	ACCTTGATCCTCCACGACGA+TGG	-	chr7_3:6795284-6795303	Msa1091500:CDS	55.0%
	TCCATCGTCGTGGAGGATCA+AGG	+	chr7_3:6795288-6795307	None:intergenic	55.0%
	TTGGGATTCCATCGTCGTGG+AGG	+	chr7_3:6795295-6795314	None:intergenic	55.0%
	TTGAACTCCGGCGCTGTTGG+TGG	-	chr7_3:6795499-6795518	Msa1091500:intron	60.0%
!!	GGAGGCACCACCAACAGCGC+CGG	+	chr7_3:6795509-6795528	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_3	gene	6795230	6795627	6795230	ID=Msa1091500;Name=Msa1091500
chr7_3	mRNA	6795230	6795627	6795230	ID=Msa1091500-mRNA-1;Parent=Msa1091500;Name=Msa1091500-mRNA-1;_AED=0.42;_eAED=0.42;_QI=21\|1\|0.5\|1\|1\|1\|2\|0\|101
chr7_3	exon	6795230	6795466	6795230	ID=Msa1091500-mRNA-1:exon:2169;Parent=Msa1091500-mRNA-1
chr7_3	exon	6795538	6795627	6795538	ID=Msa1091500-mRNA-1:exon:2168;Parent=Msa1091500-mRNA-1
chr7_3	five_prime_UTR	6795607	6795627	6795607	ID=Msa1091500-mRNA-1:five_prime_utr;Parent=Msa1091500-mRNA-1
chr7_3	CDS	6795538	6795606	6795538	ID=Msa1091500-mRNA-1:cds;Parent=Msa1091500-mRNA-1
chr7_3	CDS	6795230	6795466	6795230	ID=Msa1091500-mRNA-1:cds;Parent=Msa1091500-mRNA-1

Gene Sequence	>Msa1091500 ATGAGTTCCGGCGAGTTGGGTTGCATTTACGCTACCTTGATCCTCCACGACGATGGAATCCCAATCACCGCGGAGAAGATCGGCACTTTGTTGAAGGCTGCTAACGTCACCGTTGAATCCTACTGGCCAAACCTATTCGCTAAGCTTGCTCAGAGCAAGAACATTGATGATCTCATTTTGAACTCCGGCGCTGTTGGTGGTGCCTCCGTTGCTAAGCTTGCTCATGCTGATTCGTTTTCTTCGTCTTCTTCTTTTTCTCCTCGTTCCTTTTCAGTTAGTTCTTTTTCAAATCTTGTTGATAATTGA
Protein sequence	>Msa1091500 MSSGELGCIYATLILHDDGIPITAEKIGTLLKAANVTVESYWPNLFAKLAQSKNIDDLILNSGAVGGASVAKLAHADSFSSSSSFSPRSFSVSSFSNLVDN*