AlfalfaGEDB | Msa0939570

M. sativa cultivar zhongmu-4 / Msa0939570

KEGG: -
GO: -
PFAM: PF01582.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0939570	sp\|V9M398\|RUN1_VITRO	41.235	405	219	11	28	423	27	421	8.54e-80	278

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0939570	A0A2K3L608	73.574	333	80	2	28	360	29	353	4.09e-169	502

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0171850	Msa0939570	0.806271	8.921585e-50	-8.615850e-47
Msa0358510	Msa0939570	0.804531	2.066227e-49	-8.615850e-47
Msa0534080	Msa0939570	0.803626	3.186817e-49	-8.615850e-47
Msa0939570	Msa1253810	0.802097	6.592974e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0939570	MtrunA17_Chr6g0455211	61.985	413	134	5	27	416	38	450	1.86e-162	492
Msa0939570	MtrunA17_Chr6g0454851	68.857	350	97	2	25	363	86	434	1.95e-161	491
Msa0939570	MtrunA17_Chr6g0454931	64.835	364	104	2	28	367	50	413	6.44e-160	478
Msa0939570	MtrunA17_Chr6g0455131	66.571	347	112	1	25	367	84	430	8.76e-159	485
Msa0939570	MtrunA17_Chr6g0454951	66.102	354	109	1	25	367	79	432	3.29e-158	483
Msa0939570	MtrunA17_Chr6g0454821	66.667	348	108	3	27	367	40	386	1.79e-153	471
Msa0939570	MtrunA17_Chr4g0005851	67.059	340	111	1	28	367	37	375	1.04e-152	468
Msa0939570	MtrunA17_Chr6g0455221	65.598	343	117	1	27	368	10	352	2.25e-152	468
Msa0939570	MtrunA17_Chr6g0454971	65.875	337	113	1	27	363	48	382	3.10e-151	463
Msa0939570	MtrunA17_Chr6g0455191	62.366	372	134	2	27	394	316	685	6.22e-150	467

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0939570	AT5G41750.1	42.730	337	178	6	28	360	13	338	9.96e-72	250
Msa0939570	AT5G41750.2	42.730	337	178	6	28	360	13	338	9.96e-72	250
Msa0939570	AT5G18360.1	41.349	341	182	6	28	363	16	343	1.26e-71	248
Msa0939570	AT5G41540.1	42.121	330	176	5	28	352	11	330	5.80e-70	245
Msa0939570	AT5G17680.2	37.562	402	233	7	29	424	19	408	4.18e-69	244
Msa0939570	AT3G44480.2	37.883	359	200	7	28	373	98	446	4.42e-69	243
Msa0939570	AT1G27170.1	38.872	337	202	3	23	359	9	341	4.45e-69	244
Msa0939570	AT3G44480.4	37.883	359	200	7	28	373	98	446	4.52e-69	243
Msa0939570	AT3G44480.1	37.883	359	200	7	28	373	98	446	4.52e-69	243
Msa0939570	AT5G58120.1	39.881	336	189	4	28	359	15	341	4.78e-69	242

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Find 121 sgRNAs with CRISPR-Local

Find 376 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTATGCGGATTCACGA+TGG	0.664718	6_3:-11681668	Msa0939570:CDS
AAACATATACTTACAGCAAA+TGG	0.417141	6_3:-11679344	Msa0939570:CDS
AAATGGAGAGAGGCTCTTCG+TGG	0.559891	6_3:-11681468	Msa0939570:CDS
AACTATCCACATCATCAAGC+AGG	0.550675	6_3:+11679444	None:intergenic
AAGATATCAAACATCGACTC+TGG	0.471831	6_3:-11679512	Msa0939570:CDS
AAGGCTCTACACTCTTCGAA+TGG	0.561615	6_3:-11676600	Msa0939570:CDS
AATATCAAATGCTACAGTTG+TGG	0.580099	6_3:-11676232	Msa0939570:CDS
AATTGCCTAAACGCAATACA+CGG	0.649694	6_3:-11676812	Msa0939570:CDS
ACAGAATATTCTTAAAGAGA+AGG	0.567304	6_3:-11676619	Msa0939570:CDS
ACCAGTTTGGTGACGAACCT+CGG	0.563340	6_3:+11681560	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGCATAAAGATGTGAAATGA+AGG	+	chr6_3:11676139-11676158	None:intergenic	30.0%
TATGCTTCATTTCAAAACGT+CGG	-	chr6_3:11676153-11676172	Msa0939570:CDS	30.0%
AGAAAAGTAAGTCACTCAAA+TGG	-	chr6_3:11676466-11676485	Msa0939570:CDS	30.0%
TTGTCAAATTCAACCACAAA+TGG	-	chr6_3:11676689-11676708	Msa0939570:CDS	30.0%
TGTAGAAAAAGTCATACTAG+GGG	-	chr6_3:11676717-11676736	Msa0939570:CDS	30.0%
TTTCAAATCAGAGTTCTATG+TGG	-	chr6_3:11676839-11676858	Msa0939570:CDS	30.0%
TGAGATATTGTGTTTAGTTG+AGG	-	chr6_3:11676988-11677007	Msa0939570:intron	30.0%
GAGATATTGTGTTTAGTTGA+GGG	-	chr6_3:11676989-11677008	Msa0939570:intron	30.0%
AGATATTGTGTTTAGTTGAG+GGG	-	chr6_3:11676990-11677009	Msa0939570:intron	30.0%
AACTGCAATATAAAATGCAC+TGG	-	chr6_3:11677110-11677129	Msa0939570:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	11676012	11681961	11676012	ID=Msa0939570;Name=Msa0939570
chr6_3	mRNA	11676012	11681961	11676012	ID=Msa0939570-mRNA-1;Parent=Msa0939570;Name=Msa0939570-mRNA-1;_AED=0.95;_eAED=0.95;_QI=0\|0\|0\|0.25\|1\|1\|4\|0\|611
chr6_3	exon	11681429	11681961	11681429	ID=Msa0939570-mRNA-1:exon:3826;Parent=Msa0939570-mRNA-1
chr6_3	exon	11679334	11679877	11679334	ID=Msa0939570-mRNA-1:exon:3825;Parent=Msa0939570-mRNA-1
chr6_3	exon	11676586	11676858	11676586	ID=Msa0939570-mRNA-1:exon:3824;Parent=Msa0939570-mRNA-1
chr6_3	exon	11676012	11676497	11676012	ID=Msa0939570-mRNA-1:exon:3823;Parent=Msa0939570-mRNA-1
chr6_3	CDS	11681429	11681961	11681429	ID=Msa0939570-mRNA-1:cds;Parent=Msa0939570-mRNA-1
chr6_3	CDS	11679334	11679877	11679334	ID=Msa0939570-mRNA-1:cds;Parent=Msa0939570-mRNA-1
chr6_3	CDS	11676586	11676858	11676586	ID=Msa0939570-mRNA-1:cds;Parent=Msa0939570-mRNA-1
chr6_3	CDS	11676012	11676497	11676012	ID=Msa0939570-mRNA-1:cds;Parent=Msa0939570-mRNA-1

Gene Sequence

>Msa0939570

ATGGCAAATGGCGGACTCTCGATTGAAAGTTTATGCCAGCGTTTGGGCTTTCCAAACGGATACATACCATTCAACAGTCTGCATGAAGTGTTTTTAAGTTTTAGAGGCAAAGATACTCGTTCTTCCTTCATTTCACATCTTTATGCTTCATTTCAAAACGTCGGGGTTAAAGCTTTCATGGATGAAGATTCGATTCAAAGAGGAGGTCATATTTCAACATTGTTACCGAAAGAAATTGAACAATCTAAAATTTCTATTGTTGTTTTTTCAAAAAATTATGCGGATTCACGATGGTGTCTCAATGAGTTGGTGAAGATAATGGAGTGTCACAGAACCAAAGCACAGATAGTGCTACCAGTGTTCTATGATGTTGATCCCTCCGAGGTTCGTCACCAAACTGGTGAGTTTGGACAAGCTTTTGAAAGTCTCTTGAGCAAACTTTCGAAGAAGAAGAAGAAAAGTAAGTCACTCAAATGGAGAGAGGCTCTTCGTGGTGCTGCTGCCTTTGCTGGCTTTGTGGTTTTAAACTCCAGGAATGAAAGGGAGGCAATCGATCATATTGTTGAAAAAGTTACTATTTTGCTTGACAAGACAGACTTATTCGTCGTTGATAATCCAGTGGGAGTCAAGTCTCGAGTGCACGATACGATTCAATTACTAAACATTCAACAATCAAATGATGTTCTGCTACTAGGGATTTGGGGTATGGGGGGAATTGGTAAAACAACCATTGCAAAAGCCGTTTACAATGAAATCTCTCGCAATTTCAGTGGTAGGAGTTTCCTTGCAAATATTAGGGAAACCTGGGAGCATACTGATGGAAAAGTGTCTTTACAAGAACAACTTCTGTTTGATATCTTAAAAAAAACAACTACCAAGATATCAAACATCGACTCTGGGAAAAATACATTAAAGGATATGCTCTGCGATAAGAGAGTACTTCTCCTGCTTGATGATGTGGATAGTTTGGACCAACTAGATGCTTTGTGTGGAAGTCGTAAATGGTTTGGTTCAGGGACTAGAATAATCATTACAACCAGAGATAAACATATACTTACAGCAAATGGAGTTGATCAGATAAGGGCAATGTTAAGAAAAGATAATTGCCTAAACGCAATACACGGAAGACATGCAGATATCACTAACGAAAAGTGGAAAGAGATGGATGATAATGCCGTTGATAATTTGCACCTAGCAATGGCAGACTCGATATTGTCAAGTATTGCTGAAAAGAAGACAGCAAAGGAGATTTGGGATACTCTAATAAAATTGTACGAGGTCAAATCACTCCACAACAGAATATTCTTAAAGAGAAGGCTCTACACTCTTCGAATGGGTGAATCCACATCCAGTTTACCAGATTCGTATGACCAACTCATCATCAATATTACAAATAATAACATAGTTGACACTCTTCACTTTGATGATGTCGCCGGTGCAATCCTTGAAGAAGAATCCAGGTGCAAGAATAAGGAAGAAAGGTCAGAGAGTTCAAAGCAAGCAGAGGCTTTAACGATGACGAGAGGTAGATCAACGGAATGTGGTCCCAGTGTGAGTCAAAATCATGGTAGGTCAAAATCTCGAAGAAAGAAGAATATCAAATGCTACAGTTGTGGCATGAGAGGGCACCTAAAGAAGGAGTGTTGGAATGTCAAGAAGAATGCAGAGAAGAATTTTGAAGCTTCAACATCTCAAGGATGTGTTGCAAGTACCTCAGATGACGGGGAAATCCTGTATAGTGAAGCAGCAACTAGTTCTAAAGGTGGAAGACGACTCAACGATGTCTGGATAAAGGATTCAGGTGCAACATGGCACATGACTCCACACCGAGATTAG

Protein sequence

>Msa0939570

MANGGLSIESLCQRLGFPNGYIPFNSLHEVFLSFRGKDTRSSFISHLYASFQNVGVKAFMDEDSIQRGGHISTLLPKEIEQSKISIVVFSKNYADSRWCLNELVKIMECHRTKAQIVLPVFYDVDPSEVRHQTGEFGQAFESLLSKLSKKKKKSKSLKWREALRGAAAFAGFVVLNSRNEREAIDHIVEKVTILLDKTDLFVVDNPVGVKSRVHDTIQLLNIQQSNDVLLLGIWGMGGIGKTTIAKAVYNEISRNFSGRSFLANIRETWEHTDGKVSLQEQLLFDILKKTTTKISNIDSGKNTLKDMLCDKRVLLLLDDVDSLDQLDALCGSRKWFGSGTRIIITTRDKHILTANGVDQIRAMLRKDNCLNAIHGRHADITNEKWKEMDDNAVDNLHLAMADSILSSIAEKKTAKEIWDTLIKLYEVKSLHNRIFLKRRLYTLRMGESTSSLPDSYDQLIINITNNNIVDTLHFDDVAGAILEEESRCKNKEERSESSKQAEALTMTRGRSTECGPSVSQNHGRSKSRRKKNIKCYSCGMRGHLKKECWNVKKNAEKNFEASTSQGCVASTSDDGEILYSEAATSSKGGRRLNDVWIKDSGATWHMTPHRD*