AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0534080


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0534080 MtrunA17_Chr4g0001601 96.154 52 2 0 1 52 1 52 6.23e-32 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0534080 AT1G16000.1 42.857 56 28 1 1 52 1 56 3.30e-11 53.9

Find 11 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAGCGGCGAAGGTGGATT+TGG 0.337625 4_1:-9443292 Msa0534080:CDS
CTTACTTATAAACTATGTTC+CGG 0.434677 4_1:+9442922 None:intergenic
GCGAAGGTGGATTTGGATCA+AGG 0.444650 4_1:-9443285 Msa0534080:CDS
GGCATCATCATATCCAATAT+AGG 0.449136 4_1:+9442943 None:intergenic
GCAAAAGTGAAGAGCGGCGA+AGG 0.559375 4_1:-9443301 Msa0534080:CDS
TATATTGGATATGATGATGC+CGG 0.563965 4_1:-9442941 Msa0534080:CDS
AAAGTGAAGAGCGGCGAAGG+TGG 0.576268 4_1:-9443298 Msa0534080:CDS
CCATGGTTTCTTATGAACAG+AGG 0.615850 4_1:-9443184 Msa0534080:intron
TTGAATCAAATTCTATATAG+CGG 0.633543 4_1:-9443262 Msa0534080:CDS
CCTCTGTTCATAAGAAACCA+TGG 0.639570 4_1:+9443184 None:intergenic
GCAAAAGCAAAAGTGAAGAG+CGG 0.660636 4_1:-9443307 Msa0534080:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGGTTTATATCAATAAT+GGG - chr4_1:9443160-9443179 Msa0534080:intron 15.0%
!!! AAAAGTTATTTCTATAATTG+AGG + chr4_1:9443274-9443293 None:intergenic 15.0%
!! TTGAATCAAATTCTATATAG+CGG - chr4_1:9442984-9443003 Msa0534080:intron 20.0%
!! GTTTCAAAGTTATTAACTTT+AGG - chr4_1:9443143-9443162 Msa0534080:intron 20.0%
!!! CTTTAGGTTTATATCAATAA+TGG - chr4_1:9443159-9443178 Msa0534080:intron 20.0%
! CTATAATTGAGGTTGAAATA+AGG + chr4_1:9443263-9443282 None:intergenic 25.0%
!! TTTTGTTAAGAGTCCTATAT+TGG - chr4_1:9443290-9443309 Msa0534080:CDS 25.0%
AAACCATGGTACAGAAAAAA+CGG + chr4_1:9443051-9443070 None:intergenic 30.0%
! TATATTGGATATGATGATGC+CGG - chr4_1:9443305-9443324 Msa0534080:CDS 30.0%
GGCATCATCATATCCAATAT+AGG + chr4_1:9443306-9443325 None:intergenic 35.0%
GCAAAAGCAAAAGTGAAGAG+CGG - chr4_1:9442939-9442958 Msa0534080:CDS 40.0%
CCTCTGTTCATAAGAAACCA+TGG + chr4_1:9443065-9443084 None:intergenic 40.0%
CCATGGTTTCTTATGAACAG+AGG - chr4_1:9443062-9443081 Msa0534080:intron 40.0%
!! GAGAAGAAGCATGTTTTCGC+TGG - chr4_1:9443008-9443027 Msa0534080:intron 45.0%
!!! CTGCCGTTTTTTCTGTACCA+TGG - chr4_1:9443045-9443064 Msa0534080:intron 45.0%
! GCGAAGGTGGATTTGGATCA+AGG - chr4_1:9442961-9442980 Msa0534080:CDS 50.0%
GCAAAAGTGAAGAGCGGCGA+AGG - chr4_1:9442945-9442964 Msa0534080:CDS 55.0%
AAAGTGAAGAGCGGCGAAGG+TGG - chr4_1:9442948-9442967 Msa0534080:CDS 55.0%
! AAGAGCGGCGAAGGTGGATT+TGG - chr4_1:9442954-9442973 Msa0534080:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 9442927 9443341 9442927 ID=Msa0534080;Name=Msa0534080
chr4_1 mRNA 9442927 9443341 9442927 ID=Msa0534080-mRNA-1;Parent=Msa0534080;Name=Msa0534080-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|0.5|1|1|2|0|71
chr4_1 exon 9443185 9443341 9443185 ID=Msa0534080-mRNA-1:exon:3095;Parent=Msa0534080-mRNA-1
chr4_1 exon 9442927 9442985 9442927 ID=Msa0534080-mRNA-1:exon:3094;Parent=Msa0534080-mRNA-1
chr4_1 CDS 9443185 9443341 9443185 ID=Msa0534080-mRNA-1:cds;Parent=Msa0534080-mRNA-1
chr4_1 CDS 9442927 9442985 9442927 ID=Msa0534080-mRNA-1:cds;Parent=Msa0534080-mRNA-1
Gene Sequence

>Msa0534080

ATGAGCAACGAAGCAAAAGCAAAAGTGAAGAGCGGCGAAGGTGGATTTGGATCAAGGTTGAATCAAATTCTATATAGCGGTGAGAAGAAGCATGTTTTCGCTGGCTTAGTTCTCATCACTGCCGTTTTTTCTGTACCATGGTTTCTTATGAACAGAGAAATAACTTTTGTTAAGAGTCCTATATTGGATATGATGATGCCGGAACATAGTTTATAA

Protein sequence

>Msa0534080

MSNEAKAKVKSGEGGFGSRLNQILYSGEKKHVFAGLVLITAVFSVPWFLMNREITFVKSPILDMMMPEHSL*