AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0958570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0958570 MtrunA17_Chr7g0237021 69.186 172 53 0 1 172 1 172 6.75e-90 259
Msa0958570 MtrunA17_Chr7g0236941 69.186 172 53 0 1 172 1 172 6.75e-90 259
Msa0958570 MtrunA17_Chr7g0236971 69.186 172 53 0 1 172 230 401 3.18e-87 261
Msa0958570 MtrunA17_Chr7g0237011 69.186 172 53 0 1 172 327 498 3.76e-86 261
Msa0958570 MtrunA17_Chr7g0236961 69.186 172 53 0 1 172 327 498 3.89e-86 261
Msa0958570 MtrunA17_Chr7g0236991 69.186 172 53 0 1 172 382 553 1.25e-85 261
Msa0958570 MtrunA17_Chr7g0237001 69.186 172 53 0 1 172 382 553 1.27e-85 261
Msa0958570 MtrunA17_Chr7g0237041 69.186 172 53 0 1 172 382 553 1.27e-85 261
Msa0958570 MtrunA17_Chr7g0237081 69.186 172 53 0 1 172 382 553 1.27e-85 261
Msa0958570 MtrunA17_Chr7g0237051 69.186 172 53 0 1 172 382 553 1.27e-85 261
Msa0958570 MtrunA17_Chr4g0024691 69.186 172 53 0 1 172 390 561 6.01e-84 257
Msa0958570 MtrunA17_Chr6g0465941 65.116 172 60 0 1 172 1 172 3.00e-83 243
Msa0958570 MtrunA17_Chr6g0466121 65.116 172 60 0 1 172 1 172 3.00e-83 243
Msa0958570 MtrunA17_Chr6g0465991 65.116 172 60 0 1 172 1 172 3.00e-83 243
Msa0958570 MtrunA17_Chr6g0466091 65.116 172 60 0 1 172 1 172 3.00e-83 243
Msa0958570 MtrunA17_Chr6g0465881 64.535 172 61 0 1 172 1 172 2.13e-82 241
Msa0958570 MtrunA17_Chr6g0466031 65.116 172 60 0 1 172 89 260 5.42e-82 243
Msa0958570 MtrunA17_Chr6g0465441 66.279 172 58 0 1 172 382 553 4.81e-80 247
Msa0958570 MtrunA17_Chr5g0445501 62.209 172 65 0 1 172 1 172 1.15e-79 234
Msa0958570 MtrunA17_Chr6g0465851 65.116 172 60 0 1 172 347 518 1.84e-79 244
Msa0958570 MtrunA17_Chr6g0465911 65.116 172 60 0 1 172 382 553 5.48e-79 244
Msa0958570 MtrunA17_Chr6g0466051 65.116 172 60 0 1 172 382 553 5.66e-79 244
Msa0958570 MtrunA17_Chr3g0082691 62.791 172 64 0 1 172 382 553 4.29e-78 242
Msa0958570 MtrunA17_Chr3g0105071 63.953 172 62 0 1 172 386 557 7.96e-78 241
Msa0958570 MtrunA17_Chr5g0431651 63.529 170 62 0 1 170 381 550 1.88e-76 238
Msa0958570 MtrunA17_Chr5g0425371 65.116 172 60 0 1 172 382 553 5.61e-76 236
Msa0958570 MtrunA17_Chr6g0451711 61.628 172 66 0 1 172 386 557 6.18e-75 234
Msa0958570 MtrunA17_Chr5g0431571 61.176 170 66 0 1 170 378 547 1.79e-74 232
Msa0958570 MtrunA17_Chr7g0236921 63.924 158 53 1 19 172 1 158 3.29e-74 219
Msa0958570 MtrunA17_Chr7g0236931 61.039 154 57 1 19 172 1 151 1.93e-68 204
Msa0958570 MtrunA17_Chr7g0237061 58.140 172 49 2 1 172 89 237 2.08e-64 197
Msa0958570 MtrunA17_Chr4g0042111 55.621 169 75 0 1 169 391 559 1.98e-63 204
Msa0958570 MtrunA17_Chr2g0319041 43.860 171 93 3 3 172 389 557 6.81e-43 149
Msa0958570 MtrunA17_Chr8g0336181 39.306 173 102 3 1 172 386 556 3.68e-39 139
Msa0958570 MtrunA17_Chr2g0319071 39.181 171 101 3 1 170 319 487 5.39e-39 138
Msa0958570 MtrunA17_Chr2g0319051 38.728 173 103 3 1 172 344 514 4.91e-38 136
Msa0958570 MtrunA17_Chr3g0082711 49.138 116 42 1 57 172 1 99 4.90e-35 118
Msa0958570 MtrunA17_Chr2g0319081 38.150 173 93 4 1 172 268 427 1.14e-32 120
Msa0958570 MtrunA17_Chr4g0048921 30.769 169 112 2 1 166 399 565 2.43e-21 90.1
Msa0958570 MtrunA17_Chr7g0217361 29.762 168 118 0 3 170 141 308 3.47e-20 85.1
Msa0958570 MtrunA17_Chr3g0108941 27.647 170 117 4 1 166 346 513 5.40e-17 77.4
Msa0958570 MtrunA17_Chr4g0023911 25.882 170 126 0 1 170 410 579 6.63e-17 77.4
Msa0958570 MtrunA17_Chr6g0477121 28.477 151 100 3 1 145 263 411 1.83e-16 75.9
Msa0958570 MtrunA17_Chr3g0099031 30.000 170 113 4 1 166 345 512 3.29e-16 75.1
Msa0958570 MtrunA17_Chr4g0048911 26.036 169 117 3 1 164 391 556 3.39e-16 75.1
Msa0958570 MtrunA17_Chr2g0323151 26.144 153 111 2 1 152 311 462 3.66e-16 75.1
Msa0958570 MtrunA17_Chr3g0105691 27.922 154 107 3 1 150 378 531 9.12e-16 73.9
Msa0958570 MtrunA17_Chr3g0108991 27.922 154 107 3 1 150 345 498 1.30e-15 73.6
Msa0958570 MtrunA17_Chr3g0108951 27.922 154 107 3 1 150 345 498 1.30e-15 73.6
Msa0958570 MtrunA17_Chr3g0108971 27.922 154 107 3 1 150 320 473 1.38e-15 73.6
Msa0958570 MtrunA17_Chr3g0109031 28.205 156 104 3 1 150 345 498 2.39e-15 72.8
Msa0958570 MtrunA17_Chr3g0109001 28.477 151 100 3 1 145 346 494 2.46e-15 72.8
Msa0958570 MtrunA17_Chr3g0108981 28.477 151 100 3 1 145 321 469 3.20e-15 72.4
Msa0958570 MtrunA17_Chr3g0108961 28.477 151 100 3 1 145 321 469 3.20e-15 72.4
Msa0958570 MtrunA17_Chr6g0477111 27.329 161 109 3 1 155 320 478 4.11e-15 72.0
Msa0958570 MtrunA17_Chr3g0109011 28.205 156 104 3 1 150 320 473 4.31e-15 72.0
Msa0958570 MtrunA17_Chr3g0109021 27.815 151 101 3 1 145 345 493 1.98e-14 70.1
Msa0958570 MtrunA17_Chr2g0280581 27.168 173 113 5 1 163 349 518 1.26e-13 67.8
Msa0958570 MtrunA17_Chr2g0310401 25.595 168 124 1 3 170 264 430 5.08e-13 65.9
Msa0958570 MtrunA17_Chr2g0323131 24.419 172 125 4 1 170 395 563 2.39e-12 63.9
Msa0958570 MtrunA17_Chr4g0008631 36.145 83 51 2 91 172 1 82 7.17e-12 58.5
Msa0958570 MtrunA17_Chr2g0310331 25.000 168 125 1 3 170 418 584 8.15e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0958570 AT5G23960.1 41.279 172 100 1 1 172 377 547 4.68e-43 150
Msa0958570 AT5G23960.2 41.279 172 100 1 1 172 375 545 5.28e-43 150
Msa0958570 AT5G48110.1 31.953 169 112 3 4 172 174 339 1.23e-30 114
Msa0958570 AT1G70080.1 35.503 169 108 1 1 168 439 607 2.46e-30 115
Msa0958570 AT4G13300.1 32.353 170 114 1 4 172 385 554 5.91e-30 114
Msa0958570 AT4G20200.1 32.544 169 112 2 5 172 437 604 2.35e-29 113
Msa0958570 AT4G13280.2 31.765 170 115 1 4 172 385 554 4.84e-29 112
Msa0958570 AT3G29410.2 31.138 167 115 0 4 170 330 496 5.86e-29 111
Msa0958570 AT3G29410.1 30.769 169 117 0 4 172 435 603 1.01e-28 111
Msa0958570 AT3G32030.1 30.058 173 120 1 1 172 432 604 2.03e-28 110
Msa0958570 AT5G44630.1 31.928 166 113 0 5 170 390 555 1.19e-27 108
Msa0958570 AT1G61680.2 34.969 163 105 1 1 162 400 562 2.09e-27 107
Msa0958570 AT1G61680.1 34.969 163 105 1 1 162 407 569 2.14e-27 107
Msa0958570 AT3G14520.1 30.357 168 116 1 6 172 437 604 2.27e-27 107
Msa0958570 AT1G33750.1 29.586 169 119 0 4 172 435 603 6.56e-27 106
Msa0958570 AT3G14490.1 34.118 170 110 2 4 172 433 601 6.83e-26 103
Msa0958570 AT4G20210.1 27.811 169 122 0 4 172 432 600 1.52e-25 102
Msa0958570 AT1G31950.1 29.412 170 119 1 4 172 437 606 2.58e-25 101
Msa0958570 AT1G31950.3 29.412 170 119 1 4 172 438 607 2.69e-25 101
Msa0958570 AT3G14540.1 29.167 168 118 1 6 172 434 601 3.71e-25 101
Msa0958570 AT1G48800.1 27.273 165 120 0 4 168 438 602 1.05e-23 97.1
Msa0958570 AT4G20230.2 28.916 166 117 1 4 168 448 613 9.06e-23 94.7
Msa0958570 AT4G20230.1 28.916 166 117 1 4 168 436 601 9.35e-23 94.4
Msa0958570 AT3G29190.1 28.235 170 114 2 5 170 434 599 9.77e-23 94.4
Msa0958570 AT4G15870.1 28.571 168 119 1 6 172 431 598 1.71e-22 93.6
Msa0958570 AT4G13280.1 34.351 131 85 1 43 172 394 524 6.50e-22 92.0
Msa0958570 AT2G23230.2 28.402 169 120 1 4 171 431 599 6.56e-21 89.4
Msa0958570 AT2G23230.1 28.402 169 120 1 4 171 426 594 6.87e-21 89.0
Msa0958570 AT3G29110.1 29.341 167 118 0 4 170 429 595 2.77e-20 87.4
Msa0958570 AT1G66020.1 29.091 165 117 0 4 168 430 594 1.14e-19 85.5
Msa0958570 AT1G48820.1 25.767 163 120 1 4 165 395 557 5.35e-19 83.6
Msa0958570 AT3G25810.1 25.843 178 126 3 1 172 420 597 1.73e-14 70.9
Msa0958570 AT4G16730.1 24.855 173 127 2 1 170 365 537 2.39e-14 70.1
Msa0958570 AT4G16730.2 24.855 173 127 2 1 170 319 491 2.51e-14 70.1
Msa0958570 AT2G24210.1 23.295 176 131 3 1 172 415 590 2.30e-11 61.6
Msa0958570 AT3G25820.1 25.843 178 125 4 1 172 418 594 5.30e-11 60.5
Msa0958570 AT3G25830.1 25.843 178 125 4 1 172 418 594 5.30e-11 60.5

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTATACATTGCAGGCTT+AGG 0.276774 6_3:+72652441 Msa0958570:CDS
TGAGGGAATATGATATGAAT+AGG 0.340079 6_3:+72652643 Msa0958570:CDS
ATGCTGCTGCTATACATTGC+AGG 0.354080 6_3:+72652435 Msa0958570:CDS
AGGACATGTTTCTTCGTACT+TGG 0.361077 6_3:+72652611 Msa0958570:CDS
CGTACTTGGAATGTTATATG+AGG 0.388936 6_3:+72652625 Msa0958570:CDS
CGACAAAAGACATCTTTGAT+TGG 0.390521 6_3:+72652387 Msa0958570:CDS
TGCATGTACTCCTCTGTTGT+TGG 0.418606 6_3:-72652296 None:intergenic
ATAAATACTCAAATTTCTAA+AGG 0.433133 6_3:-72652876 None:intergenic
GACAAAAGACATCTTTGATT+GGG 0.442878 6_3:+72652388 Msa0958570:CDS
AAAAGAAAATTGACAACGCT+TGG 0.444020 6_3:+72652688 Msa0958570:CDS
TGCTATACATTGCAGGCTTA+GGG 0.448464 6_3:+72652442 Msa0958570:CDS
TACGACTTCTTACATTGGAA+TGG 0.454510 6_3:+72652355 Msa0958570:CDS
ATAGTTAGCATTCAGCCATC+TGG 0.458951 6_3:-72652270 None:intergenic
ACGACTTCTTACATTGGAAT+GGG 0.460983 6_3:+72652356 Msa0958570:CDS
GAGGGAATATGATATGAATA+GGG 0.475500 6_3:+72652644 Msa0958570:CDS
CGCTTGGAAAGATATAAATC+AGG 0.490061 6_3:+72652704 Msa0958570:CDS
TTGAGAACACACGTCAGAAT+AGG 0.502783 6_3:-72652756 None:intergenic
TAAGAAGTCGTAGCCAACAC+TGG 0.522936 6_3:-72652344 None:intergenic
ATAGGTAACGGAACTTCAGT+TGG 0.538099 6_3:-72652738 None:intergenic
AAACAGTTTGAGCAGAAAAG+AGG 0.539017 6_3:+72652591 Msa0958570:intron
CTTATATGAGTGAGGCCAGA+TGG 0.549142 6_3:+72652255 None:intergenic
GTACTTGGAATGTTATATGA+GGG 0.567173 6_3:+72652626 Msa0958570:CDS
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6_3:+72652331 Msa0958570:CDS
TTGGCTACGACTTCTTACAT+TGG 0.586299 6_3:+72652350 Msa0958570:CDS
CACATATCAACAATATCAAG+TGG 0.594145 6_3:+72652317 Msa0958570:CDS
AAGAAGTCGTAGCCAACACT+GGG 0.613680 6_3:-72652343 None:intergenic
GATAACTACACACACTCGGA+CGG 0.620946 6_3:+72652816 Msa0958570:CDS
TACAGATAACTACACACACT+CGG 0.627170 6_3:+72652812 Msa0958570:CDS
AATCCAAGCTTATATGAGTG+AGG 0.644710 6_3:+72652247 None:intergenic
GGATGAAATTGTGTCAAGCG+AGG 0.666273 6_3:+72652463 Msa0958570:CDS
ACACACGTCAGAATAGGTAA+CGG 0.688917 6_3:-72652750 None:intergenic
TAACTATATACCAACAACAG+AGG 0.703519 6_3:+72652286 Msa0958570:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAACTATATACCAACAACAG+AGG + chr6_3:72652286-72652305 Msa0958570:CDS 30.0%
CACATATCAACAATATCAAG+TGG + chr6_3:72652317-72652336 Msa0958570:CDS 30.0%
GACAAAAGACATCTTTGATT+GGG + chr6_3:72652388-72652407 Msa0958570:CDS 30.0%
GTACTTGGAATGTTATATGA+GGG + chr6_3:72652626-72652645 Msa0958570:CDS 30.0%
TGAGGGAATATGATATGAAT+AGG + chr6_3:72652643-72652662 Msa0958570:CDS 30.0%
GAGGGAATATGATATGAATA+GGG + chr6_3:72652644-72652663 Msa0958570:CDS 30.0%
! TTTCTTTTGGCATTCTTGAA+TGG - chr6_3:72652676-72652695 None:intergenic 30.0%
!! AAGCGTTGTCAATTTTCTTT+TGG - chr6_3:72652689-72652708 None:intergenic 30.0%
AAAAGAAAATTGACAACGCT+TGG + chr6_3:72652688-72652707 Msa0958570:CDS 30.0%
TACGACTTCTTACATTGGAA+TGG + chr6_3:72652355-72652374 Msa0958570:CDS 35.0%
ACGACTTCTTACATTGGAAT+GGG + chr6_3:72652356-72652375 Msa0958570:CDS 35.0%
CGACAAAAGACATCTTTGAT+TGG + chr6_3:72652387-72652406 Msa0958570:CDS 35.0%
! GCAGCAGCATTAACAATTTT+TGG - chr6_3:72652425-72652444 None:intergenic 35.0%
AAACAGTTTGAGCAGAAAAG+AGG + chr6_3:72652591-72652610 Msa0958570:intron 35.0%
CGTACTTGGAATGTTATATG+AGG + chr6_3:72652625-72652644 Msa0958570:CDS 35.0%
CGCTTGGAAAGATATAAATC+AGG + chr6_3:72652704-72652723 Msa0958570:CDS 35.0%
TACAGATAACTACACACACT+CGG + chr6_3:72652812-72652831 Msa0958570:CDS 35.0%
ATAGTTAGCATTCAGCCATC+TGG - chr6_3:72652273-72652292 None:intergenic 40.0%
TTGGCTACGACTTCTTACAT+TGG + chr6_3:72652350-72652369 Msa0958570:CDS 40.0%
TGCTATACATTGCAGGCTTA+GGG + chr6_3:72652442-72652461 Msa0958570:CDS 40.0%
AGGACATGTTTCTTCGTACT+TGG + chr6_3:72652611-72652630 Msa0958570:CDS 40.0%
!! ATAGGTAACGGAACTTCAGT+TGG - chr6_3:72652741-72652760 None:intergenic 40.0%
ACACACGTCAGAATAGGTAA+CGG - chr6_3:72652753-72652772 None:intergenic 40.0%
TTGAGAACACACGTCAGAAT+AGG - chr6_3:72652759-72652778 None:intergenic 40.0%
TGCATGTACTCCTCTGTTGT+TGG - chr6_3:72652299-72652318 None:intergenic 45.0%
ATCAAGTGGTTACCCAGTGT+TGG + chr6_3:72652331-72652350 Msa0958570:CDS 45.0%
AAGAAGTCGTAGCCAACACT+GGG - chr6_3:72652346-72652365 None:intergenic 45.0%
TAAGAAGTCGTAGCCAACAC+TGG - chr6_3:72652347-72652366 None:intergenic 45.0%
ATGCTGCTGCTATACATTGC+AGG + chr6_3:72652435-72652454 Msa0958570:CDS 45.0%
CTGCTATACATTGCAGGCTT+AGG + chr6_3:72652441-72652460 Msa0958570:CDS 45.0%
GGATGAAATTGTGTCAAGCG+AGG + chr6_3:72652463-72652482 Msa0958570:CDS 45.0%
! CAATCTCTCGCGTTTTATCG+AGG + chr6_3:72652776-72652795 Msa0958570:CDS 45.0%
GATAACTACACACACTCGGA+CGG + chr6_3:72652816-72652835 Msa0958570:CDS 45.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 72652260 72652890 72652260 ID=Msa0958570;Name=Msa0958570
chr6_3 mRNA 72652260 72652890 72652260 ID=Msa0958570-mRNA-1;Parent=Msa0958570;Name=Msa0958570-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|172
chr6_3 exon 72652260 72652484 72652260 ID=Msa0958570-mRNA-1:exon:13254;Parent=Msa0958570-mRNA-1
chr6_3 exon 72652597 72652890 72652597 ID=Msa0958570-mRNA-1:exon:13255;Parent=Msa0958570-mRNA-1
chr6_3 CDS 72652260 72652484 72652260 ID=Msa0958570-mRNA-1:cds;Parent=Msa0958570-mRNA-1
chr6_3 CDS 72652597 72652890 72652597 ID=Msa0958570-mRNA-1:cds;Parent=Msa0958570-mRNA-1
Gene Sequence

>Msa0958570

ATGAGTGAGGCCAGATGGCTGAATGCTAACTATATACCAACAACAGAGGAGTACATGCACATATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTCTTACATTGGAATGGGAAACATAGCGACAAAAGACATCTTTGATTGGGTAACTAACAATCCAAAAATTGTTAATGCTGCTGCTATACATTGCAGGCTTAGGGATGAAATTGTGTCAAGCGAGTTTGAGCAGAAAAGAGGACATGTTTCTTCGTACTTGGAATGTTATATGAGGGAATATGATATGAATAGGGAAGCTGCCATTCAAGAATGCCAAAAGAAAATTGACAACGCTTGGAAAGATATAAATCAGGAATGTCTTAAGCCAACTGAAGTTCCGTTACCTATTCTGACGTGTGTTCTCAATCTCTCGCGTTTTATCGAGGTTTTTTACAAAGATACAGATAACTACACACACTCGGACGGACTAATGAAGACATATATTAATGATTTGCTTGTCAATCCTTTAGAAATTTGA

Protein sequence

>Msa0958570

MSEARWLNANYIPTTEEYMHISTISSGYPVLATTSYIGMGNIATKDIFDWVTNNPKIVNAAAIHCRLRDEIVSSEFEQKRGHVSSYLECYMREYDMNREAAIQECQKKIDNAWKDINQECLKPTEVPLPILTCVLNLSRFIEVFYKDTDNYTHSDGLMKTYINDLLVNPLEI*