AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0920830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0920830 MtrunA17_Chr7g0237021 68.605 172 54 0 1 172 1 172 2.30e-88 256
Msa0920830 MtrunA17_Chr7g0236941 68.605 172 54 0 1 172 1 172 2.30e-88 256
Msa0920830 MtrunA17_Chr7g0236971 68.605 172 54 0 1 172 230 401 1.10e-85 257
Msa0920830 MtrunA17_Chr7g0237011 68.605 172 54 0 1 172 327 498 1.37e-84 257
Msa0920830 MtrunA17_Chr7g0236961 68.605 172 54 0 1 172 327 498 1.66e-84 257
Msa0920830 MtrunA17_Chr7g0237001 68.605 172 54 0 1 172 382 553 5.34e-84 257
Msa0920830 MtrunA17_Chr7g0237041 68.605 172 54 0 1 172 382 553 5.34e-84 257
Msa0920830 MtrunA17_Chr7g0237081 68.605 172 54 0 1 172 382 553 5.34e-84 257
Msa0920830 MtrunA17_Chr7g0237051 68.605 172 54 0 1 172 382 553 5.34e-84 257
Msa0920830 MtrunA17_Chr7g0236991 68.605 172 54 0 1 172 382 553 5.82e-84 257
Msa0920830 MtrunA17_Chr4g0024691 68.605 172 54 0 1 172 390 561 2.96e-82 253
Msa0920830 MtrunA17_Chr6g0465941 63.953 172 62 0 1 172 1 172 3.12e-81 238
Msa0920830 MtrunA17_Chr6g0466121 63.953 172 62 0 1 172 1 172 3.12e-81 238
Msa0920830 MtrunA17_Chr6g0465991 63.953 172 62 0 1 172 1 172 3.12e-81 238
Msa0920830 MtrunA17_Chr6g0466091 63.953 172 62 0 1 172 1 172 3.12e-81 238
Msa0920830 MtrunA17_Chr6g0465881 63.372 172 63 0 1 172 1 172 1.86e-80 236
Msa0920830 MtrunA17_Chr6g0466031 63.953 172 62 0 1 172 89 260 6.07e-80 238
Msa0920830 MtrunA17_Chr5g0445501 62.209 172 65 0 1 172 1 172 4.18e-79 232
Msa0920830 MtrunA17_Chr6g0465441 65.116 172 60 0 1 172 382 553 5.73e-78 241
Msa0920830 MtrunA17_Chr6g0465851 63.953 172 62 0 1 172 347 518 1.81e-77 239
Msa0920830 MtrunA17_Chr6g0465911 63.953 172 62 0 1 172 382 553 5.44e-77 239
Msa0920830 MtrunA17_Chr6g0466051 63.953 172 62 0 1 172 382 553 5.61e-77 239
Msa0920830 MtrunA17_Chr3g0082691 62.209 172 65 0 1 172 382 553 1.96e-76 238
Msa0920830 MtrunA17_Chr3g0105071 63.372 172 63 0 1 172 386 557 3.48e-76 237
Msa0920830 MtrunA17_Chr5g0431651 62.209 172 65 0 1 172 381 552 7.79e-75 233
Msa0920830 MtrunA17_Chr5g0425371 64.535 172 61 0 1 172 382 553 3.10e-74 232
Msa0920830 MtrunA17_Chr6g0451711 61.047 172 67 0 1 172 386 557 3.33e-73 229
Msa0920830 MtrunA17_Chr5g0431571 60.588 170 67 0 1 170 378 547 8.21e-73 228
Msa0920830 MtrunA17_Chr7g0236921 63.291 158 54 1 19 172 1 158 1.28e-72 215
Msa0920830 MtrunA17_Chr7g0236931 60.390 154 58 1 19 172 1 151 6.55e-67 201
Msa0920830 MtrunA17_Chr7g0237061 57.558 172 50 2 1 172 89 237 8.01e-63 193
Msa0920830 MtrunA17_Chr4g0042111 54.438 169 77 0 1 169 391 559 2.77e-61 198
Msa0920830 MtrunA17_Chr2g0319041 43.860 171 93 3 3 172 389 557 3.18e-42 148
Msa0920830 MtrunA17_Chr8g0336181 39.306 173 102 3 1 172 386 556 2.52e-38 137
Msa0920830 MtrunA17_Chr2g0319071 39.181 171 101 3 1 170 319 487 2.90e-38 136
Msa0920830 MtrunA17_Chr2g0319051 38.728 173 103 3 1 172 344 514 2.61e-37 134
Msa0920830 MtrunA17_Chr3g0082711 48.276 116 43 1 57 172 1 99 1.90e-33 114
Msa0920830 MtrunA17_Chr2g0319081 38.150 173 93 4 1 172 268 427 7.51e-32 118
Msa0920830 MtrunA17_Chr4g0048921 30.058 173 116 2 1 170 399 569 5.26e-21 89.0
Msa0920830 MtrunA17_Chr7g0217361 29.940 167 117 0 3 169 141 307 9.81e-21 86.7
Msa0920830 MtrunA17_Chr3g0108941 28.235 170 116 4 1 166 346 513 2.72e-17 78.2
Msa0920830 MtrunA17_Chr4g0023911 25.882 170 126 0 1 170 410 579 7.58e-17 77.0
Msa0920830 MtrunA17_Chr6g0477121 29.139 151 99 3 1 145 263 411 1.18e-16 76.3
Msa0920830 MtrunA17_Chr3g0105691 28.571 154 106 3 1 150 378 531 5.92e-16 74.3
Msa0920830 MtrunA17_Chr3g0108971 28.571 154 106 3 1 150 320 473 7.70e-16 74.3
Msa0920830 MtrunA17_Chr3g0108991 28.571 154 106 3 1 150 345 498 9.14e-16 73.9
Msa0920830 MtrunA17_Chr3g0108951 28.571 154 106 3 1 150 345 498 9.14e-16 73.9
Msa0920830 MtrunA17_Chr3g0109031 29.139 151 99 3 1 145 345 493 1.51e-15 73.2
Msa0920830 MtrunA17_Chr3g0109001 29.139 151 99 3 1 145 346 494 1.60e-15 73.2
Msa0920830 MtrunA17_Chr3g0108981 29.139 151 99 3 1 145 321 469 1.85e-15 73.2
Msa0920830 MtrunA17_Chr3g0108961 29.139 151 99 3 1 145 321 469 1.85e-15 73.2
Msa0920830 MtrunA17_Chr4g0048911 26.036 169 117 3 1 164 391 556 2.34e-15 72.8
Msa0920830 MtrunA17_Chr3g0099031 30.000 170 113 4 1 166 345 512 2.67e-15 72.8
Msa0920830 MtrunA17_Chr3g0109011 29.139 151 99 3 1 145 320 468 2.77e-15 72.4
Msa0920830 MtrunA17_Chr6g0477111 27.950 161 108 3 1 155 320 478 2.93e-15 72.4
Msa0920830 MtrunA17_Chr2g0280581 27.746 173 112 5 1 163 349 518 1.05e-14 70.9
Msa0920830 MtrunA17_Chr3g0109021 28.477 151 100 3 1 145 345 493 1.28e-14 70.5
Msa0920830 MtrunA17_Chr2g0323151 25.490 153 112 2 1 152 311 462 1.43e-14 70.5
Msa0920830 MtrunA17_Chr2g0310401 26.190 168 123 1 3 170 264 430 7.27e-14 68.6
Msa0920830 MtrunA17_Chr2g0310331 25.595 168 124 1 3 170 418 584 2.33e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0920830 AT5G23960.1 40.116 172 102 1 1 172 377 547 4.70e-41 145
Msa0920830 AT5G23960.2 40.116 172 102 1 1 172 375 545 5.94e-41 144
Msa0920830 AT1G70080.1 34.682 173 112 1 1 172 439 611 1.00e-29 114
Msa0920830 AT5G48110.1 31.953 169 112 3 4 172 174 339 1.25e-29 111
Msa0920830 AT4G13300.1 31.176 170 116 1 4 172 385 554 7.69e-28 108
Msa0920830 AT1G61680.1 35.583 163 104 1 1 162 407 569 9.27e-28 108
Msa0920830 AT1G61680.2 35.583 163 104 1 1 162 400 562 9.91e-28 108
Msa0920830 AT4G20200.1 31.361 169 114 2 5 172 437 604 2.60e-27 107
Msa0920830 AT4G13280.2 31.361 169 115 1 5 172 386 554 3.21e-27 107
Msa0920830 AT3G29410.2 29.586 169 119 0 4 172 330 498 4.53e-27 106
Msa0920830 AT3G29410.1 29.586 169 119 0 4 172 435 603 6.37e-27 106
Msa0920830 AT3G32030.1 28.902 173 122 1 1 172 432 604 1.34e-26 105
Msa0920830 AT3G14520.1 30.233 172 111 3 6 172 437 604 8.60e-26 103
Msa0920830 AT5G44630.1 30.723 166 115 0 5 170 390 555 1.10e-25 102
Msa0920830 AT1G33750.1 28.402 169 121 0 4 172 435 603 1.42e-25 102
Msa0920830 AT3G14490.1 32.941 170 112 2 4 172 433 601 3.66e-24 98.6
Msa0920830 AT1G31950.3 29.310 174 114 3 4 172 438 607 1.20e-23 97.1
Msa0920830 AT1G31950.1 29.310 174 114 3 4 172 437 606 1.21e-23 97.1
Msa0920830 AT4G20210.1 26.627 169 124 0 4 172 432 600 1.63e-23 96.7
Msa0920830 AT3G14540.1 29.070 172 113 3 6 172 434 601 1.71e-23 96.7
Msa0920830 AT3G29190.1 28.488 172 115 2 5 172 434 601 4.25e-23 95.5
Msa0920830 AT1G48800.1 27.273 165 120 0 4 168 438 602 1.48e-22 94.0
Msa0920830 AT4G20230.2 28.313 166 118 1 4 168 448 613 8.77e-22 91.7
Msa0920830 AT4G20230.1 28.313 166 118 1 4 168 436 601 9.23e-22 91.7
Msa0920830 AT4G15870.1 27.381 168 121 1 6 172 431 598 1.68e-20 88.2
Msa0920830 AT4G13280.1 33.588 131 86 1 43 172 394 524 4.15e-20 86.7
Msa0920830 AT2G23230.2 29.091 165 116 1 4 167 431 595 6.97e-20 86.3
Msa0920830 AT2G23230.1 29.091 165 116 1 4 167 426 590 7.30e-20 86.3
Msa0920830 AT1G66020.1 27.811 169 122 0 4 172 430 598 4.86e-19 84.0
Msa0920830 AT3G29110.1 28.144 167 120 0 4 170 429 595 3.30e-18 81.3
Msa0920830 AT1G48820.1 24.540 163 122 1 4 165 395 557 7.34e-17 77.4
Msa0920830 AT3G25810.1 26.966 178 124 4 1 172 420 597 1.29e-14 71.2
Msa0920830 AT4G16730.1 26.012 173 125 2 1 170 365 537 1.39e-14 70.9
Msa0920830 AT4G16730.2 25.714 175 127 2 1 172 319 493 1.55e-14 70.9
Msa0920830 AT2G24210.1 23.295 176 131 3 1 172 415 590 3.77e-12 63.9
Msa0920830 AT3G25820.1 25.843 178 125 4 1 172 418 594 6.18e-11 60.5
Msa0920830 AT3G25830.1 25.843 178 125 4 1 172 418 594 6.18e-11 60.5

Find 29 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTATACATTGCAGGCTT+AGG 0.276774 6_2:+56428171 Msa0920830:CDS
TGAGGGAATATGATATGAAT+AGG 0.340079 6_2:+56428373 Msa0920830:CDS
ATGCTGCTGCTATACATTGC+AGG 0.354080 6_2:+56428165 Msa0920830:CDS
CGTACTTAGAATGTTATATG+AGG 0.388941 6_2:+56428355 Msa0920830:CDS
CGACAAAAGACATCTTTGAT+TGG 0.390521 6_2:+56428117 Msa0920830:CDS
TGCATGTACTCCTCTGTTGT+TGG 0.418606 6_2:-56428026 None:intergenic
ATAAATACTCAAATTTCTAC+AGG 0.441751 6_2:-56428606 None:intergenic
GACAAAAGACATCTTTGATT+GGG 0.442878 6_2:+56428118 Msa0920830:CDS
AAAAGAAAATTGACAACGCT+TGG 0.444020 6_2:+56428418 Msa0920830:CDS
TGCTATACATTGCAGGCTTA+GGG 0.448464 6_2:+56428172 Msa0920830:CDS
TACGACTTCTTACATTGGAA+TGG 0.454510 6_2:+56428085 Msa0920830:CDS
ATAGTTAGCATTCAGCCATC+TGG 0.458951 6_2:-56428000 None:intergenic
ACGACTTCTTACATTGGAAT+GGG 0.460983 6_2:+56428086 Msa0920830:CDS
GAGGGAATATGATATGAATA+GGG 0.475500 6_2:+56428374 Msa0920830:CDS
CGCTTGGAAAGATATAAATC+AGG 0.490061 6_2:+56428434 Msa0920830:CDS
TTGAGAACACACGTCAGAAT+AGG 0.502783 6_2:-56428486 None:intergenic
TAAGAAGTCGTAGCCAACAC+TGG 0.522936 6_2:-56428074 None:intergenic
AAACAGTTTGAGCAGAAAAG+AGG 0.539017 6_2:+56428321 Msa0920830:intron
CTTATATGAGTGAGGCCAGA+TGG 0.549142 6_2:+56427985 None:intergenic
ATAGGTAACAGAACTTCAGT+TGG 0.561871 6_2:-56428468 None:intergenic
ATCAAGTGGTTACCCAGTGT+TGG 0.570889 6_2:+56428061 Msa0920830:CDS
GTACTTAGAATGTTATATGA+GGG 0.573263 6_2:+56428356 Msa0920830:CDS
TTGGCTACGACTTCTTACAT+TGG 0.586299 6_2:+56428080 Msa0920830:CDS
CACATATCAACAATATCAAG+TGG 0.594145 6_2:+56428047 Msa0920830:CDS
GATAACTACACACACTCTGA+CGG 0.610210 6_2:+56428546 Msa0920830:CDS
GGATGAAATTGTGTCAAGCA+AGG 0.613036 6_2:+56428193 Msa0920830:CDS
AAGAAGTCGTAGCCAACACT+GGG 0.613680 6_2:-56428073 None:intergenic
AATCCAAGCTTATATGAGTG+AGG 0.644710 6_2:+56427977 None:intergenic
TAACTATATACCAACAACAG+AGG 0.703519 6_2:+56428016 Msa0920830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTACTTAGAATGTTATATGA+GGG + chr6_2:56428356-56428375 Msa0920830:CDS 25.0%
TAACTATATACCAACAACAG+AGG + chr6_2:56428016-56428035 Msa0920830:CDS 30.0%
CACATATCAACAATATCAAG+TGG + chr6_2:56428047-56428066 Msa0920830:CDS 30.0%
GACAAAAGACATCTTTGATT+GGG + chr6_2:56428118-56428137 Msa0920830:CDS 30.0%
CGTACTTAGAATGTTATATG+AGG + chr6_2:56428355-56428374 Msa0920830:CDS 30.0%
TGAGGGAATATGATATGAAT+AGG + chr6_2:56428373-56428392 Msa0920830:CDS 30.0%
GAGGGAATATGATATGAATA+GGG + chr6_2:56428374-56428393 Msa0920830:CDS 30.0%
! TTTCTTTTGGCATTCTTGAA+TGG - chr6_2:56428406-56428425 None:intergenic 30.0%
!! AAGCGTTGTCAATTTTCTTT+TGG - chr6_2:56428419-56428438 None:intergenic 30.0%
AAAAGAAAATTGACAACGCT+TGG + chr6_2:56428418-56428437 Msa0920830:CDS 30.0%
TACGACTTCTTACATTGGAA+TGG + chr6_2:56428085-56428104 Msa0920830:CDS 35.0%
ACGACTTCTTACATTGGAAT+GGG + chr6_2:56428086-56428105 Msa0920830:CDS 35.0%
CGACAAAAGACATCTTTGAT+TGG + chr6_2:56428117-56428136 Msa0920830:CDS 35.0%
! GCAGCAGCATTAACAATTTT+TGG - chr6_2:56428155-56428174 None:intergenic 35.0%
AAACAGTTTGAGCAGAAAAG+AGG + chr6_2:56428321-56428340 Msa0920830:intron 35.0%
CGCTTGGAAAGATATAAATC+AGG + chr6_2:56428434-56428453 Msa0920830:CDS 35.0%
ATAGGTAACAGAACTTCAGT+TGG - chr6_2:56428471-56428490 None:intergenic 35.0%
ATAGTTAGCATTCAGCCATC+TGG - chr6_2:56428003-56428022 None:intergenic 40.0%
TTGGCTACGACTTCTTACAT+TGG + chr6_2:56428080-56428099 Msa0920830:CDS 40.0%
TGCTATACATTGCAGGCTTA+GGG + chr6_2:56428172-56428191 Msa0920830:CDS 40.0%
GGATGAAATTGTGTCAAGCA+AGG + chr6_2:56428193-56428212 Msa0920830:CDS 40.0%
TTGAGAACACACGTCAGAAT+AGG - chr6_2:56428489-56428508 None:intergenic 40.0%
!! CAATCTCTCGCGTTTTATTG+AGG + chr6_2:56428506-56428525 Msa0920830:CDS 40.0%
GATAACTACACACACTCTGA+CGG + chr6_2:56428546-56428565 Msa0920830:CDS 40.0%
TGCATGTACTCCTCTGTTGT+TGG - chr6_2:56428029-56428048 None:intergenic 45.0%
ATCAAGTGGTTACCCAGTGT+TGG + chr6_2:56428061-56428080 Msa0920830:CDS 45.0%
AAGAAGTCGTAGCCAACACT+GGG - chr6_2:56428076-56428095 None:intergenic 45.0%
TAAGAAGTCGTAGCCAACAC+TGG - chr6_2:56428077-56428096 None:intergenic 45.0%
ATGCTGCTGCTATACATTGC+AGG + chr6_2:56428165-56428184 Msa0920830:CDS 45.0%
CTGCTATACATTGCAGGCTT+AGG + chr6_2:56428171-56428190 Msa0920830:CDS 45.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 56427990 56428620 56427990 ID=Msa0920830;Name=Msa0920830
chr6_2 mRNA 56427990 56428620 56427990 ID=Msa0920830-mRNA-1;Parent=Msa0920830;Name=Msa0920830-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|172
chr6_2 exon 56427990 56428214 56427990 ID=Msa0920830-mRNA-1:exon:8461;Parent=Msa0920830-mRNA-1
chr6_2 exon 56428327 56428620 56428327 ID=Msa0920830-mRNA-1:exon:8462;Parent=Msa0920830-mRNA-1
chr6_2 CDS 56427990 56428214 56427990 ID=Msa0920830-mRNA-1:cds;Parent=Msa0920830-mRNA-1
chr6_2 CDS 56428327 56428620 56428327 ID=Msa0920830-mRNA-1:cds;Parent=Msa0920830-mRNA-1
Gene Sequence

>Msa0920830

ATGAGTGAGGCCAGATGGCTGAATGCTAACTATATACCAACAACAGAGGAGTACATGCACATATCAACAATATCAAGTGGTTACCCAGTGTTGGCTACGACTTCTTACATTGGAATGGGAAACATAGCGACAAAAGACATCTTTGATTGGGTAACTAACAATCCAAAAATTGTTAATGCTGCTGCTATACATTGCAGGCTTAGGGATGAAATTGTGTCAAGCAAGTTTGAGCAGAAAAGAGGACATGTTTCTTCGTACTTAGAATGTTATATGAGGGAATATGATATGAATAGGGAAGCTGCCATTCAAGAATGCCAAAAGAAAATTGACAACGCTTGGAAAGATATAAATCAGGAATGTCTTAAGCCAACTGAAGTTCTGTTACCTATTCTGACGTGTGTTCTCAATCTCTCGCGTTTTATTGAGGTTTTTTACAAAGATACAGATAACTACACACACTCTGACGGACTAATGAAGACATATATTAATGATTTGCTTGTCAATCCTGTAGAAATTTGA

Protein sequence

>Msa0920830

MSEARWLNANYIPTTEEYMHISTISSGYPVLATTSYIGMGNIATKDIFDWVTNNPKIVNAAAIHCRLRDEIVSSKFEQKRGHVSSYLECYMREYDMNREAAIQECQKKIDNAWKDINQECLKPTEVLLPILTCVLNLSRFIEVFYKDTDNYTHSDGLMKTYINDLLVNPVEI*