AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0959540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0959540 MtrunA17_Chr8g0353391 67.347 49 5 1 130 178 36 73 3.00e-15 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 105 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTAGTGTAGTTGATTT+TGG 0.172373 6_3:-76439022 Msa0959540:three_prime_UTR
AATCTATCAATTATAGTCTT+TGG 0.224672 6_3:-76438384 Msa0959540:three_prime_UTR
ACCTGCATTTCAATCTAAAT+TGG 0.230713 6_3:+76438098 None:intergenic
TCCTTGGTCTTCAAGTGTTT+TGG 0.233477 6_3:-76439124 Msa0959540:three_prime_UTR
TGTTGACATAAGTAAATATT+GGG 0.252074 6_3:-76438261 Msa0959540:three_prime_UTR
ACCTTAGATTTCAAGAATTT+AGG 0.274679 6_3:-76437544 Msa0959540:three_prime_UTR
TCTCTTGGTTCTTATTTCCT+TGG 0.283416 6_3:-76438970 Msa0959540:three_prime_UTR
ATGATTTAGGTTTATTTCAT+TGG 0.286261 6_3:-76440001 Msa0959540:intron
AGAATTTAGGATTCTAACTA+TGG 0.294002 6_3:-76437531 Msa0959540:three_prime_UTR
CAGAGTAGGTTGTTGTAGTT+TGG 0.295852 6_3:-76439045 Msa0959540:three_prime_UTR
TTGAATGTTCTTTCTTTGCT+TGG 0.297109 6_3:-76438181 Msa0959540:three_prime_UTR
CTCTTGGTTCTTATTTCCTT+GGG 0.302979 6_3:-76438969 Msa0959540:three_prime_UTR
GGAAACTTATTAACAACATT+TGG 0.312904 6_3:-76438070 Msa0959540:three_prime_UTR
AATAACAAACAAAAGCAATT+AGG 0.313480 6_3:-76437768 Msa0959540:three_prime_UTR
TTGTTGACATAAGTAAATAT+TGG 0.313550 6_3:-76438262 Msa0959540:three_prime_UTR
TGTATTTGAGAGTATAAGTT+TGG 0.316023 6_3:-76438739 Msa0959540:three_prime_UTR
ATTGTTAAAAGTACGATAAA+TGG 0.327098 6_3:+76437922 None:intergenic
TCGATGGAGCTTGAGCTTAC+TGG 0.328079 6_3:-76439967 Msa0959540:CDS
TGCATAATATAAGTAAGGTT+TGG 0.329020 6_3:-76437708 Msa0959540:three_prime_UTR
CGGCGAGGGTGGAGGATTGC+TGG 0.330889 6_3:-76441289 Msa0959540:CDS
GTGTCAAGGAAGCAAATTTA+AGG 0.333717 6_3:-76438803 Msa0959540:three_prime_UTR
TGTGTTGTTGTCAGCTTCAT+AGG 0.339794 6_3:-76438614 Msa0959540:three_prime_UTR
TTATGTAGTCTCAAAACTAT+AGG 0.341547 6_3:-76438880 Msa0959540:three_prime_UTR
GATGGTTTCTTATGTTCTCT+TGG 0.351285 6_3:-76438985 Msa0959540:three_prime_UTR
AAGGGCGATGAGAGCGATTC+CGG 0.356983 6_3:-76441387 Msa0959540:CDS
TCCTAAATTCTTGAAATCTA+AGG 0.361062 6_3:+76437543 None:intergenic
ATAGTACATGATCATTGAAT+TGG 0.361664 6_3:-76438779 Msa0959540:three_prime_UTR
TCCGTTGCGGCTGATGAATC+TGG 0.361892 6_3:+76441112 None:intergenic
GATATGGAATGAGCCATTCT+TGG 0.369248 6_3:+76438542 None:intergenic
GTAATGCAGCAGCGGTGTTT+TGG 0.370709 6_3:-76439241 Msa0959540:CDS
GCATAATATAAGTAAGGTTT+GGG 0.379089 6_3:-76437707 Msa0959540:three_prime_UTR
GGCTATAGTATTATATTCTC+TGG 0.385111 6_3:-76438586 Msa0959540:three_prime_UTR
CGTCAACATTGAACATATTC+CGG 0.385663 6_3:+76441081 None:intergenic
AAATAAGTTATGAACCTTAG+TGG 0.390097 6_3:-76438845 Msa0959540:three_prime_UTR
TAGTACATGATCATTGAATT+GGG 0.393326 6_3:-76438778 Msa0959540:three_prime_UTR
CATGTCGTCTTCAATTGATA+TGG 0.406032 6_3:+76438526 None:intergenic
AACTATTCAGAGTTGGTTCA+AGG 0.418221 6_3:-76439343 Msa0959540:CDS
AGATTGAAATGCAGGTCATA+TGG 0.419303 6_3:-76438091 Msa0959540:three_prime_UTR
GCCAATTTAGATTGAAATGC+AGG 0.450947 6_3:-76438099 Msa0959540:three_prime_UTR
CAGAGACAAATTAGAAGGTA+TGG 0.450960 6_3:+76439185 None:intergenic
AGAGTATAAGTTTGGAATTG+AGG 0.457699 6_3:-76438731 Msa0959540:three_prime_UTR
CGAGGTCGCAACAGTAGATT+CGG 0.464707 6_3:+76441042 None:intergenic
CAGTAGATTCGGTTGTTCTC+CGG 0.469266 6_3:+76441053 None:intergenic
AACAATGCATAATATAAGTA+AGG 0.469552 6_3:-76437713 Msa0959540:three_prime_UTR
TGCGCTAGCAGCGGCGAGGG+TGG 0.470828 6_3:-76441300 Msa0959540:CDS
ATCGATTTAAGTGTGAACTT+TGG 0.478948 6_3:-76437969 Msa0959540:three_prime_UTR
CATCGTGAACTTAGCTCAGT+TGG 0.483129 6_3:-76437740 Msa0959540:three_prime_UTR
CAACTCAGTTGGACCAAGAA+TGG 0.487133 6_3:-76438555 Msa0959540:three_prime_UTR
TGGAACAGAGACAAATTAGA+AGG 0.494862 6_3:+76439180 None:intergenic
GCATGCGCTAGCAGCGGCGA+GGG 0.496549 6_3:-76441303 Msa0959540:CDS
ATGCATTATTTATCACTATG+AGG 0.498995 6_3:-76437946 Msa0959540:three_prime_UTR
AACTTATTAACAACATTTGG+AGG 0.499331 6_3:-76438067 Msa0959540:three_prime_UTR
ATATGACCCGTGCGGTAGCA+CGG 0.503386 6_3:-76437828 Msa0959540:three_prime_UTR
GCAAAGAAAGAACATTCAAA+AGG 0.511770 6_3:+76438185 None:intergenic
TGGAGGCATTTGTTGTTACA+TGG 0.512320 6_3:-76438050 Msa0959540:three_prime_UTR
CTCCCTCCCGTGCTACCGCA+CGG 0.516126 6_3:+76437821 None:intergenic
CATAATTTCGAGCGAAGTCG+TGG 0.516262 6_3:-76439310 Msa0959540:CDS
GCTAGCAGCGGCGAGGGTGG+AGG 0.519133 6_3:-76441297 Msa0959540:CDS
TATGACCCGTGCGGTAGCAC+GGG 0.522349 6_3:-76437827 Msa0959540:three_prime_UTR
AATTTAAAATAACTACCAAC+AGG 0.522429 6_3:+76438463 None:intergenic
GTTCAATGTTGACGAGAGAC+CGG 0.524464 6_3:-76441072 Msa0959540:CDS
GAGATTAACGGACGCGGTGA+AGG 0.528559 6_3:-76441406 Msa0959540:CDS
TAAAACCGACATGTTGCAGC+TGG 0.532167 6_3:+76439160 None:intergenic
TCCCTCCCGTGCTACCGCAC+GGG 0.539928 6_3:+76437822 None:intergenic
CCAATTTAGATTACTTTGTG+TGG 0.541221 6_3:-76438121 Msa0959540:intron
GATGAGAGCGATTCCGGTGA+AGG 0.544387 6_3:-76441381 Msa0959540:CDS
GCCGACGCAGACACCTTCAC+CGG 0.547300 6_3:+76441368 None:intergenic
TGTGCTTAACTATTCAGAGT+TGG 0.553654 6_3:-76439350 Msa0959540:CDS
ACTGGATATCAATATGTTGG+TGG 0.553704 6_3:-76439949 Msa0959540:CDS
ATTGGAGAAGATGCCATCGA+TGG 0.555013 6_3:-76439983 Msa0959540:CDS
GACCCGTGCGGTAGCACGGG+AGG 0.560454 6_3:-76437824 Msa0959540:three_prime_UTR
TTGACTCACATAAGAACTCA+AGG 0.560539 6_3:+76437621 None:intergenic
TCCGGTGGTTTCATCCGTTG+CGG 0.563463 6_3:+76441099 None:intergenic
CGACCTCGCTAACGTACCCT+AGG 0.564837 6_3:-76441027 Msa0959540:intron
TCCAGATTCATCAGCCGCAA+CGG 0.566551 6_3:-76441113 Msa0959540:CDS
TGCATGCGCTAGCAGCGGCG+AGG 0.570502 6_3:-76441304 Msa0959540:CDS
ATATCAATATGTTGGTGGGC+CGG 0.570699 6_3:-76439944 Msa0959540:CDS
GTTGACATAAGTAAATATTG+GGG 0.576108 6_3:-76438260 Msa0959540:three_prime_UTR
TCCGGTGAAGGTGTCTGCGT+CGG 0.576477 6_3:-76441369 Msa0959540:CDS
TGGAGGATTGCTGGAGACAA+TGG 0.576512 6_3:-76441280 Msa0959540:intron
AGATTAACGGACGCGGTGAA+GGG 0.586989 6_3:-76441405 Msa0959540:CDS
CTCTTTGCATGCGCTAGCAG+CGG 0.592887 6_3:-76441309 Msa0959540:CDS
TCACAACAACTAATCCACTA+AGG 0.593194 6_3:+76438831 None:intergenic
TTGTTGTGAGCAGCGTGTCA+AGG 0.594775 6_3:-76438817 Msa0959540:three_prime_UTR
CTTACTGGATATCAATATGT+TGG 0.596237 6_3:-76439952 Msa0959540:CDS
CTGTTCCAGCTGCAACATGT+CGG 0.597509 6_3:-76439165 Msa0959540:three_prime_UTR
CTGGATATCAATATGTTGGT+GGG 0.599065 6_3:-76439948 Msa0959540:CDS
ACCCGTGCGGTAGCACGGGA+GGG 0.607528 6_3:-76437823 Msa0959540:three_prime_UTR
AGGTGTAAGTCAACTGACAA+AGG 0.614363 6_3:-76438711 Msa0959540:three_prime_UTR
TGAAATAAATGTGCTCCTGT+TGG 0.614759 6_3:-76438478 Msa0959540:three_prime_UTR
GGTGGTGACAGTGAGATTAA+CGG 0.616433 6_3:-76441418 Msa0959540:CDS
GTAAGCTCAAGCTCCATCGA+TGG 0.619771 6_3:+76439970 None:intergenic
GCCGCAACGGATGAAACCAC+CGG 0.626069 6_3:-76441100 Msa0959540:CDS
AAACCTAGGGTACGTTAGCG+AGG 0.629611 6_3:+76441024 None:intergenic
GTGAACTTAGCTCAGTTGGT+AGG 0.633143 6_3:-76437736 Msa0959540:three_prime_UTR
AGTACATGATCATTGAATTG+GGG 0.638741 6_3:-76438777 Msa0959540:three_prime_UTR
AGACAAATTAGAAGGTATGG+AGG 0.643311 6_3:+76439188 None:intergenic
CAACATTGAACATATTCCGG+TGG 0.660528 6_3:+76441084 None:intergenic
GCCAAAACACTTGAAGACCA+AGG 0.674145 6_3:+76439123 None:intergenic
GTAGCACTAAAAGAAACCCA+AGG 0.688804 6_3:+76438953 None:intergenic
GACAGTGAGATTAACGGACG+CGG 0.704201 6_3:-76441412 Msa0959540:CDS
AGGACACCAATACAACCAGT+CGG 0.704600 6_3:+76439078 None:intergenic
GGACTAATGTAATGCAGCAG+CGG 0.726893 6_3:-76439249 Msa0959540:CDS
GGGTCATATATATTTAACAG+CGG 0.731209 6_3:+76437842 None:intergenic
TTAAATATATATGACCCGTG+CGG 0.792223 6_3:-76437836 Msa0959540:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTTTTACATGATTT+AGG - chr6_3:76437992-76438011 Msa0959540:three_prime_UTR 10.0%
!! GATTAAAAAAAATGATAAAA+TGG + chr6_3:76439087-76439106 None:intergenic 10.0%
!!! ATATCATTATCATAAATTTT+AGG - chr6_3:76439254-76439273 Msa0959540:CDS 10.0%
!!! AATTTTTTTCTTTGTTATAA+AGG - chr6_3:76440281-76440300 Msa0959540:intron 10.0%
!!! TTTTGATCAATTTAATTTTA+AGG - chr6_3:76440704-76440723 Msa0959540:intron 10.0%
!!! AGTATAATCTAATTTTTTTT+TGG + chr6_3:76441256-76441275 None:intergenic 10.0%
!! AATCATGTAAAAAAAATAAG+AGG + chr6_3:76437991-76438010 None:intergenic 15.0%
!!! TCAAATGCATTTTATTTTAA+AGG - chr6_3:76438192-76438211 Msa0959540:three_prime_UTR 15.0%
!!! TAATTTTCTATTTAGTTGAA+GGG + chr6_3:76438611-76438630 None:intergenic 15.0%
!!! ATAATTTTCTATTTAGTTGA+AGG + chr6_3:76438612-76438631 None:intergenic 15.0%
!! TCAACTAAATAGAAAATTAT+AGG - chr6_3:76438612-76438631 Msa0959540:three_prime_UTR 15.0%
!! CAACTAAATAGAAAATTATA+GGG - chr6_3:76438613-76438632 Msa0959540:three_prime_UTR 15.0%
!! TATCAGAAATAAAATGATAA+TGG + chr6_3:76438816-76438835 None:intergenic 15.0%
!! AATATATGTTATCATGATTT+AGG - chr6_3:76438907-76438926 Msa0959540:three_prime_UTR 15.0%
!!! ATCATTTTTTTTAATCAGTT+TGG - chr6_3:76439091-76439110 Msa0959540:three_prime_UTR 15.0%
!!! ATCATTTTATCATTTATGTT+AGG - chr6_3:76439144-76439163 Msa0959540:three_prime_UTR 15.0%
!!! TATTCTATAAGCATATATTT+TGG + chr6_3:76439522-76439541 None:intergenic 15.0%
!!! ATAATCTAATTTTTTTTTGG+TGG + chr6_3:76441253-76441272 None:intergenic 15.0%
!!! TCTTCTTCTATCTTTAATTT+TGG - chr6_3:76438058-76438077 Msa0959540:three_prime_UTR 20.0%
!!! ACATTTGAAACAATATATCA+AGG + chr6_3:76438091-76438110 None:intergenic 20.0%
!! TTGATATATTGTTTCAAATG+TGG - chr6_3:76438090-76438109 Msa0959540:three_prime_UTR 20.0%
!! TTCAAAAAGGTTTGAAAAAT+CGG - chr6_3:76438275-76438294 Msa0959540:three_prime_UTR 20.0%
!!! TTTATCTTTTGGAAAATCAA+TGG + chr6_3:76438457-76438476 None:intergenic 20.0%
!!! TGATTTTCCAAAAGATAAAA+AGG - chr6_3:76438458-76438477 Msa0959540:three_prime_UTR 20.0%
!!! TATGTTACCTTTTTATCTTT+TGG + chr6_3:76438468-76438487 None:intergenic 20.0%
!!! ACATATATCATCTTTTTATC+TGG - chr6_3:76438483-76438502 Msa0959540:three_prime_UTR 20.0%
!!! AATTTTCTATTTAGTTGAAG+GGG + chr6_3:76438610-76438629 None:intergenic 20.0%
!!! CTTAAAATCATTTAATTTCC+TGG + chr6_3:76438668-76438687 None:intergenic 20.0%
!!! ATGATTTAGGTTTATTTCAT+TGG - chr6_3:76438920-76438939 Msa0959540:three_prime_UTR 20.0%
!! TTAAATACTTAAAGAAGAAC+AGG + chr6_3:76439038-76439057 None:intergenic 20.0%
!! TTGAAAGAAAAAGATACAAT+TGG - chr6_3:76439214-76439233 Msa0959540:exon 20.0%
!! TGAAAGAAAAAGATACAATT+GGG - chr6_3:76439215-76439234 Msa0959540:exon 20.0%
!!! GTTCATAACTTATTTTTGTA+AGG + chr6_3:76440071-76440090 None:intergenic 20.0%
!!! TGAAGACTTTTCAAAAAATA+TGG + chr6_3:76440262-76440281 None:intergenic 20.0%
!!! AATTTAAAATAACTACCAAC+AGG + chr6_3:76440461-76440480 None:intergenic 20.0%
!!! TGTTGGTAGTTATTTTAAAT+TGG - chr6_3:76440460-76440479 Msa0959540:intron 20.0%
!! AATCTATCAATTATAGTCTT+TGG - chr6_3:76440537-76440556 Msa0959540:intron 20.0%
!! TTGTTGACATAAGTAAATAT+TGG - chr6_3:76440659-76440678 Msa0959540:intron 20.0%
!! TGTTGACATAAGTAAATATT+GGG - chr6_3:76440660-76440679 Msa0959540:intron 20.0%
!!! ATTGTTAAAAGTACGATAAA+TGG + chr6_3:76441002-76441021 None:intergenic 20.0%
!!! CTTTTGTTTGTTATTATAAG+TGG + chr6_3:76441148-76441167 None:intergenic 20.0%
!! AATAACAAACAAAAGCAATT+AGG - chr6_3:76441153-76441172 Msa0959540:CDS 20.0%
!! AACAATGCATAATATAAGTA+AGG - chr6_3:76441208-76441227 Msa0959540:intron 20.0%
!! ATTTAGGTGATTTTTGCATT+TGG - chr6_3:76438008-76438027 Msa0959540:three_prime_UTR 25.0%
!!! CTTCTATCTTTAATTTTGGT+TGG - chr6_3:76438062-76438081 Msa0959540:three_prime_UTR 25.0%
!!! TAGCAGAAGATTTTTCAAAA+AGG - chr6_3:76438262-76438281 Msa0959540:three_prime_UTR 25.0%
! TTATGTTAAGCATATACTAC+TGG - chr6_3:76438776-76438795 Msa0959540:three_prime_UTR 25.0%
! AGTATTTAATTCTGATCACT+TGG - chr6_3:76439049-76439068 Msa0959540:three_prime_UTR 25.0%
!! TTATTTTCTTCTCTTTTCCT+TGG - chr6_3:76439781-76439800 Msa0959540:intron 25.0%
!!! AAAAAGTTTTTTTAACCGAC+TGG - chr6_3:76439828-76439847 Msa0959540:intron 25.0%
!!! TGATTTTGGTTTGTTTTCTT+TGG - chr6_3:76439913-76439932 Msa0959540:intron 25.0%
!!! GATTTTGGTTTGTTTTCTTT+GGG - chr6_3:76439914-76439933 Msa0959540:intron 25.0%
! TTATGTAGTCTCAAAACTAT+AGG - chr6_3:76440041-76440060 Msa0959540:intron 25.0%
! AAATAAGTTATGAACCTTAG+TGG - chr6_3:76440076-76440095 Msa0959540:intron 25.0%
! ATAGTACATGATCATTGAAT+TGG - chr6_3:76440142-76440161 Msa0959540:intron 25.0%
! TAGTACATGATCATTGAATT+GGG - chr6_3:76440143-76440162 Msa0959540:intron 25.0%
!! TGTATTTGAGAGTATAAGTT+TGG - chr6_3:76440182-76440201 Msa0959540:intron 25.0%
!! TTATAGTCTTTGGAAAAACA+TGG - chr6_3:76440547-76440566 Msa0959540:intron 25.0%
! GTTGACATAAGTAAATATTG+GGG - chr6_3:76440661-76440680 Msa0959540:intron 25.0%
! GGAAACTTATTAACAACATT+TGG - chr6_3:76440851-76440870 Msa0959540:intron 25.0%
! AACTTATTAACAACATTTGG+AGG - chr6_3:76440854-76440873 Msa0959540:intron 25.0%
! ATGCATTATTTATCACTATG+AGG - chr6_3:76440975-76440994 Msa0959540:intron 25.0%
! TGCATAATATAAGTAAGGTT+TGG - chr6_3:76441213-76441232 Msa0959540:intron 25.0%
! GCATAATATAAGTAAGGTTT+GGG - chr6_3:76441214-76441233 Msa0959540:intron 25.0%
! ACCTTAGATTTCAAGAATTT+AGG - chr6_3:76441377-76441396 Msa0959540:CDS 25.0%
! TCCTAAATTCTTGAAATCTA+AGG + chr6_3:76441381-76441400 None:intergenic 25.0%
!! AGAATTTAGGATTCTAACTA+TGG - chr6_3:76441390-76441409 Msa0959540:CDS 25.0%
!! AACAATAACCTTCACTTTTG+CGG + chr6_3:76437672-76437691 None:intergenic 30.0%
!!! AATGTATTTTTGAGTGAGAG+AGG + chr6_3:76437704-76437723 None:intergenic 30.0%
! TCAGATCGTCATATTTTGTA+CGG - chr6_3:76437782-76437801 Msa0959540:three_prime_UTR 30.0%
TTCTCTTGGGATTTAAATCA+CGG + chr6_3:76438152-76438171 None:intergenic 30.0%
! TTGAAATGTGTTGTTCTCTT+GGG + chr6_3:76438165-76438184 None:intergenic 30.0%
! TTTGAAATGTGTTGTTCTCT+TGG + chr6_3:76438166-76438185 None:intergenic 30.0%
TTCGATTCAATACTCAGTTA+TGG - chr6_3:76438239-76438258 Msa0959540:three_prime_UTR 30.0%
! AACATGTGACAATTCAAGTT+GGG + chr6_3:76438639-76438658 None:intergenic 30.0%
AAACATGTGACAATTCAAGT+TGG + chr6_3:76438640-76438659 None:intergenic 30.0%
TTGTCACATGTTTATAATCC+AGG - chr6_3:76438647-76438666 Msa0959540:three_prime_UTR 30.0%
TGTTAACTTCAATTATCCCT+TGG + chr6_3:76438697-76438716 None:intergenic 30.0%
! GGAATAGTTTGTGTAAGATA+TGG + chr6_3:76438854-76438873 None:intergenic 30.0%
!! TCAACTTCATATACTGGTTT+GGG + chr6_3:76438876-76438895 None:intergenic 30.0%
! TTCAACTTCATATACTGGTT+TGG + chr6_3:76438877-76438896 None:intergenic 30.0%
TGTACTTCAACTTCATATAC+TGG + chr6_3:76438882-76438901 None:intergenic 30.0%
CTTACTGGATATCAATATGT+TGG - chr6_3:76438969-76438988 Msa0959540:three_prime_UTR 30.0%
TATACAAACTCAATACATGC+CGG + chr6_3:76438999-76439018 None:intergenic 30.0%
! TCATTTTGTCTCATTCTGTT+AGG - chr6_3:76439171-76439190 Msa0959540:three_prime_UTR 30.0%
! CAATAAGAAGCATGGAATAA+AGG + chr6_3:76439448-76439467 None:intergenic 30.0%
TTGATACTCAATAAGAAGCA+TGG + chr6_3:76439456-76439475 None:intergenic 30.0%
!!! TTTTGGAGTTATGCTAACTA+AGG + chr6_3:76439505-76439524 None:intergenic 30.0%
!! TTTTCTGCATAATTTAGAGC+TGG - chr6_3:76439637-76439656 Msa0959540:intron 30.0%
!!! CATAATTTAGAGCTGGTTTT+CGG - chr6_3:76439644-76439663 Msa0959540:intron 30.0%
!! CTCTTTAGTGTAGTTGATTT+TGG - chr6_3:76439899-76439918 Msa0959540:intron 30.0%
AGTACATGATCATTGAATTG+GGG - chr6_3:76440144-76440163 Msa0959540:intron 30.0%
! AGAGTATAAGTTTGGAATTG+AGG - chr6_3:76440190-76440209 Msa0959540:intron 30.0%
GGCTATAGTATTATATTCTC+TGG - chr6_3:76440335-76440354 Msa0959540:intron 30.0%
GCAAAGAAAGAACATTCAAA+AGG + chr6_3:76440739-76440758 None:intergenic 30.0%
TTGAATGTTCTTTCTTTGCT+TGG - chr6_3:76440740-76440759 Msa0959540:intron 30.0%
CCACACAAAGTAATCTAAAT+TGG + chr6_3:76440803-76440822 None:intergenic 30.0%
CCAATTTAGATTACTTTGTG+TGG - chr6_3:76440800-76440819 Msa0959540:intron 30.0%
ACCTGCATTTCAATCTAAAT+TGG + chr6_3:76440826-76440845 None:intergenic 30.0%
!!! TAGCTTTCTATTTTAGTGTG+TGG - chr6_3:76440916-76440935 Msa0959540:intron 30.0%
!!! AGCTTTCTATTTTAGTGTGT+GGG - chr6_3:76440917-76440936 Msa0959540:intron 30.0%
! ATCGATTTAAGTGTGAACTT+TGG - chr6_3:76440952-76440971 Msa0959540:intron 30.0%
GGGTCATATATATTTAACAG+CGG + chr6_3:76441082-76441101 None:intergenic 30.0%
TTAAATATATATGACCCGTG+CGG - chr6_3:76441085-76441104 Msa0959540:CDS 30.0%
!!! CTAATTTTTTTTTGGTGGTC+AGG + chr6_3:76441248-76441267 None:intergenic 30.0%
CGTCAACATTGAACATATTC+CGG + chr6_3:76437843-76437862 None:intergenic 35.0%
! AGGTGATTTTTGCATTTGGT+TGG - chr6_3:76438012-76438031 Msa0959540:three_prime_UTR 35.0%
TACAATTACTGAGCAAACAG+AGG + chr6_3:76438345-76438364 None:intergenic 35.0%
! TTAAGTGTTTTCTCACCAAG+TGG - chr6_3:76438525-76438544 Msa0959540:three_prime_UTR 35.0%
! TAAGTGTTTTCTCACCAAGT+GGG - chr6_3:76438526-76438545 Msa0959540:three_prime_UTR 35.0%
ACGATTCATCTTCGGTTAAA+AGG + chr6_3:76438555-76438574 None:intergenic 35.0%
AATGATGCACGATTCATCTT+CGG + chr6_3:76438563-76438582 None:intergenic 35.0%
!! ATGATTTTAAGTGCCAACCA+AGG - chr6_3:76438677-76438696 Msa0959540:three_prime_UTR 35.0%
!! TGATTTTAAGTGCCAACCAA+GGG - chr6_3:76438678-76438697 Msa0959540:three_prime_UTR 35.0%
AACTTCAATTATCCCTTGGT+TGG + chr6_3:76438693-76438712 None:intergenic 35.0%
!! CAACTTCATATACTGGTTTG+GGG + chr6_3:76438875-76438894 None:intergenic 35.0%
ACTGGATATCAATATGTTGG+TGG - chr6_3:76438972-76438991 Msa0959540:three_prime_UTR 35.0%
CTGGATATCAATATGTTGGT+GGG - chr6_3:76438973-76438992 Msa0959540:three_prime_UTR 35.0%
TGTGCTTAACTATTCAGAGT+TGG - chr6_3:76439571-76439590 Msa0959540:intron 35.0%
! AACTATTCAGAGTTGGTTCA+AGG - chr6_3:76439578-76439597 Msa0959540:intron 35.0%
AAAAAACTCTGCTATGTAGC+AGG + chr6_3:76439713-76439732 None:intergenic 35.0%
! AGACAAATTAGAAGGTATGG+AGG + chr6_3:76439736-76439755 None:intergenic 35.0%
CAGAGACAAATTAGAAGGTA+TGG + chr6_3:76439739-76439758 None:intergenic 35.0%
TGGAACAGAGACAAATTAGA+AGG + chr6_3:76439744-76439763 None:intergenic 35.0%
!! TTTTAACCGACTGGTTGTAT+TGG - chr6_3:76439837-76439856 Msa0959540:intron 35.0%
!!! TTGGTTTGTTTTCTTTGGGA+TGG - chr6_3:76439918-76439937 Msa0959540:intron 35.0%
GATGGTTTCTTATGTTCTCT+TGG - chr6_3:76439936-76439955 Msa0959540:intron 35.0%
! TCTCTTGGTTCTTATTTCCT+TGG - chr6_3:76439951-76439970 Msa0959540:CDS 35.0%
! CTCTTGGTTCTTATTTCCTT+GGG - chr6_3:76439952-76439971 Msa0959540:CDS 35.0%
TCACAACAACTAATCCACTA+AGG + chr6_3:76440093-76440112 None:intergenic 35.0%
GTGTCAAGGAAGCAAATTTA+AGG - chr6_3:76440118-76440137 Msa0959540:intron 35.0%
! TTGTCAGCTTCATAGGTTTT+AGG - chr6_3:76440314-76440333 Msa0959540:intron 35.0%
! TGGATTTTGAACAACTCAGT+TGG - chr6_3:76440355-76440374 Msa0959540:intron 35.0%
CATGTCGTCTTCAATTGATA+TGG + chr6_3:76440398-76440417 None:intergenic 35.0%
TGAAATAAATGTGCTCCTGT+TGG - chr6_3:76440443-76440462 Msa0959540:intron 35.0%
!!! TTGGGGTTTTGAGTCTATTT+TGG - chr6_3:76440678-76440697 Msa0959540:intron 35.0%
GCCAATTTAGATTGAAATGC+AGG - chr6_3:76440822-76440841 Msa0959540:intron 35.0%
AGATTGAAATGCAGGTCATA+TGG - chr6_3:76440830-76440849 Msa0959540:intron 35.0%
TTGACTCACATAAGAACTCA+AGG + chr6_3:76441303-76441322 None:intergenic 35.0%
!!! TCCGTTACTTTTTCTGTGCA+GGG - chr6_3:76437749-76437768 Msa0959540:three_prime_UTR 40.0%
CAACATTGAACATATTCCGG+TGG + chr6_3:76437840-76437859 None:intergenic 40.0%
CCTAGTATTCCTCAAACCTA+GGG + chr6_3:76437913-76437932 None:intergenic 40.0%
TCCTAGTATTCCTCAAACCT+AGG + chr6_3:76437914-76437933 None:intergenic 40.0%
ATTACTGAGCAAACAGAGGA+AGG + chr6_3:76438341-76438360 None:intergenic 40.0%
! TCACCAACACGAGTTATTTC+AGG - chr6_3:76438386-76438405 Msa0959540:three_prime_UTR 40.0%
! AAGTGTTTTCTCACCAAGTG+GGG - chr6_3:76438527-76438546 Msa0959540:three_prime_UTR 40.0%
ATGAATCGTGCATCATTGCT+TGG - chr6_3:76438566-76438585 Msa0959540:three_prime_UTR 40.0%
!! CATTGCTTGGTCTTGAAGAA+TGG - chr6_3:76438579-76438598 Msa0959540:three_prime_UTR 40.0%
TGTGATGTTGAACTTGATGC+TGG - chr6_3:76438747-76438766 Msa0959540:three_prime_UTR 40.0%
! ATATCAATATGTTGGTGGGC+CGG - chr6_3:76438977-76438996 Msa0959540:three_prime_UTR 40.0%
AGTTCGTGAGATTGATATCG+AGG - chr6_3:76439287-76439306 Msa0959540:CDS 40.0%
!! TCCTTGGTCTTCAAGTGTTT+TGG - chr6_3:76439797-76439816 Msa0959540:intron 40.0%
CAGAGTAGGTTGTTGTAGTT+TGG - chr6_3:76439876-76439895 Msa0959540:intron 40.0%
GTAGCACTAAAAGAAACCCA+AGG + chr6_3:76439971-76439990 None:intergenic 40.0%
AGGTGTAAGTCAACTGACAA+AGG - chr6_3:76440210-76440229 Msa0959540:intron 40.0%
! TGTGTTGTTGTCAGCTTCAT+AGG - chr6_3:76440307-76440326 Msa0959540:intron 40.0%
GATATGGAATGAGCCATTCT+TGG + chr6_3:76440382-76440401 None:intergenic 40.0%
!! TCAAAGAGCGTTACTTTGTG+TGG - chr6_3:76440777-76440796 Msa0959540:intron 40.0%
!! TGGAGGCATTTGTTGTTACA+TGG - chr6_3:76440871-76440890 Msa0959540:intron 40.0%
GGTGGTGACAGTGAGATTAA+CGG - chr6_3:76437503-76437522 Msa0959540:three_prime_UTR 45.0%
TTGAGTGAGAGAGGAGAGAA+AGG + chr6_3:76437695-76437714 None:intergenic 45.0%
!!! CTCCGTTACTTTTTCTGTGC+AGG - chr6_3:76437748-76437767 Msa0959540:three_prime_UTR 45.0%
GCCCTGCACAGAAAAAGTAA+CGG + chr6_3:76437753-76437772 None:intergenic 45.0%
GTTCAATGTTGACGAGAGAC+CGG - chr6_3:76437849-76437868 Msa0959540:three_prime_UTR 45.0%
! CAGTAGATTCGGTTGTTCTC+CGG + chr6_3:76437871-76437890 None:intergenic 45.0%
CCCTAGGTTTGAGGAATACT+AGG - chr6_3:76437910-76437929 Msa0959540:three_prime_UTR 45.0%
CCTCCTGAAATAACTCGTGT+TGG + chr6_3:76438392-76438411 None:intergenic 45.0%
! CCAACACGAGTTATTTCAGG+AGG - chr6_3:76438389-76438408 Msa0959540:three_prime_UTR 45.0%
!! CTTGGTCTTGAAGAATGGAG+AGG - chr6_3:76438584-76438603 Msa0959540:three_prime_UTR 45.0%
TGTTGAACTTGATGCTGGCA+TGG - chr6_3:76438752-76438771 Msa0959540:three_prime_UTR 45.0%
ATTGGAGAAGATGCCATCGA+TGG - chr6_3:76438938-76438957 Msa0959540:three_prime_UTR 45.0%
CATAATTTCGAGCGAAGTCG+TGG - chr6_3:76439611-76439630 Msa0959540:intron 45.0%
GGACTAATGTAATGCAGCAG+CGG - chr6_3:76439672-76439691 Msa0959540:intron 45.0%
TAAAACCGACATGTTGCAGC+TGG + chr6_3:76439764-76439783 None:intergenic 45.0%
GCCAAAACACTTGAAGACCA+AGG + chr6_3:76439801-76439820 None:intergenic 45.0%
AGGACACCAATACAACCAGT+CGG + chr6_3:76439846-76439865 None:intergenic 45.0%
!!! TCCTGCTGCTTTTTCAGAGT+AGG - chr6_3:76439862-76439881 Msa0959540:intron 45.0%
! ACCTACTCTGAAAAAGCAGC+AGG + chr6_3:76439866-76439885 None:intergenic 45.0%
CAACTCAGTTGGACCAAGAA+TGG - chr6_3:76440366-76440385 Msa0959540:intron 45.0%
CATCGTGAACTTAGCTCAGT+TGG - chr6_3:76441181-76441200 Msa0959540:intron 45.0%
GTGAACTTAGCTCAGTTGGT+AGG - chr6_3:76441185-76441204 Msa0959540:intron 45.0%
! GACAGTGAGATTAACGGACG+CGG - chr6_3:76437509-76437528 Msa0959540:three_prime_UTR 50.0%
! AGATTAACGGACGCGGTGAA+GGG - chr6_3:76437516-76437535 Msa0959540:three_prime_UTR 50.0%
TGGAGGATTGCTGGAGACAA+TGG - chr6_3:76437641-76437660 Msa0959540:three_prime_UTR 50.0%
TGGCACAACCGCAAAAGTGA+AGG - chr6_3:76437661-76437680 Msa0959540:three_prime_UTR 50.0%
! TTTTTCTGTGCAGGGCAGTG+AGG - chr6_3:76437757-76437776 Msa0959540:three_prime_UTR 50.0%
! TTTTCTGTGCAGGGCAGTGA+GGG - chr6_3:76437758-76437777 Msa0959540:three_prime_UTR 50.0%
TCCAGATTCATCAGCCGCAA+CGG - chr6_3:76437808-76437827 Msa0959540:three_prime_UTR 50.0%
CGAGGTCGCAACAGTAGATT+CGG + chr6_3:76437882-76437901 None:intergenic 50.0%
AAACCTAGGGTACGTTAGCG+AGG + chr6_3:76437900-76437919 None:intergenic 50.0%
GCTAACGTACCCTAGGTTTG+AGG - chr6_3:76437901-76437920 Msa0959540:three_prime_UTR 50.0%
AATCGGCCTGAATGTCAACG+CGG - chr6_3:76438292-76438311 Msa0959540:three_prime_UTR 50.0%
! GACTAACCGCGTTGACATTC+AGG + chr6_3:76438301-76438320 None:intergenic 50.0%
GTAAGCTCAAGCTCCATCGA+TGG + chr6_3:76438954-76438973 None:intergenic 50.0%
TCGATGGAGCTTGAGCTTAC+TGG - chr6_3:76438954-76438973 Msa0959540:three_prime_UTR 50.0%
!!! TAGAGCTGGTTTTCGGCATG+TGG - chr6_3:76439651-76439670 Msa0959540:intron 50.0%
! GTAATGCAGCAGCGGTGTTT+TGG - chr6_3:76439680-76439699 Msa0959540:intron 50.0%
CTGTTCCAGCTGCAACATGT+CGG - chr6_3:76439756-76439775 Msa0959540:intron 50.0%
TTGTTGTGAGCAGCGTGTCA+AGG - chr6_3:76440104-76440123 Msa0959540:intron 50.0%
! GAGATTAACGGACGCGGTGA+AGG - chr6_3:76437515-76437534 Msa0959540:three_prime_UTR 55.0%
AAGGGCGATGAGAGCGATTC+CGG - chr6_3:76437534-76437553 Msa0959540:three_prime_UTR 55.0%
GATGAGAGCGATTCCGGTGA+AGG - chr6_3:76437540-76437559 Msa0959540:three_prime_UTR 55.0%
CTCTTTGCATGCGCTAGCAG+CGG - chr6_3:76437612-76437631 Msa0959540:three_prime_UTR 55.0%
TCCGTTGCGGCTGATGAATC+TGG + chr6_3:76437812-76437831 None:intergenic 55.0%
TCCGGTGGTTTCATCCGTTG+CGG + chr6_3:76437825-76437844 None:intergenic 55.0%
CGGTTAAAAGGCTCCCCACT+TGG + chr6_3:76438543-76438562 None:intergenic 55.0%
ATATGACCCGTGCGGTAGCA+CGG - chr6_3:76441093-76441112 Msa0959540:CDS 55.0%
!! TCCGGTGAAGGTGTCTGCGT+CGG - chr6_3:76437552-76437571 Msa0959540:three_prime_UTR 60.0%
GCCGCAACGGATGAAACCAC+CGG - chr6_3:76437821-76437840 Msa0959540:three_prime_UTR 60.0%
CGACCTCGCTAACGTACCCT+AGG - chr6_3:76437894-76437913 Msa0959540:three_prime_UTR 60.0%
TATGACCCGTGCGGTAGCAC+GGG - chr6_3:76441094-76441113 Msa0959540:CDS 60.0%
GCCGACGCAGACACCTTCAC+CGG + chr6_3:76437556-76437575 None:intergenic 65.0%
TGCATGCGCTAGCAGCGGCG+AGG - chr6_3:76437617-76437636 Msa0959540:three_prime_UTR 70.0%
GCATGCGCTAGCAGCGGCGA+GGG - chr6_3:76437618-76437637 Msa0959540:three_prime_UTR 70.0%
CGGCGAGGGTGGAGGATTGC+TGG - chr6_3:76437632-76437651 Msa0959540:three_prime_UTR 70.0%
ACCCGTGCGGTAGCACGGGA+GGG - chr6_3:76441098-76441117 Msa0959540:CDS 70.0%
TCCCTCCCGTGCTACCGCAC+GGG + chr6_3:76441102-76441121 None:intergenic 70.0%
CTCCCTCCCGTGCTACCGCA+CGG + chr6_3:76441103-76441122 None:intergenic 70.0%
TGCGCTAGCAGCGGCGAGGG+TGG - chr6_3:76437621-76437640 Msa0959540:three_prime_UTR 75.0%
GCTAGCAGCGGCGAGGGTGG+AGG - chr6_3:76437624-76437643 Msa0959540:three_prime_UTR 75.0%
GACCCGTGCGGTAGCACGGG+AGG - chr6_3:76441097-76441116 Msa0959540:CDS 75.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 76437501 76441442 76437501 ID=Msa0959540;Name=Msa0959540
chr6_3 mRNA 76437501 76441442 76437501 ID=Msa0959540-mRNA-1;Parent=Msa0959540;Name=Msa0959540-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0.5|0.6|1|0.75|0.6|5|1698|178
chr6_3 exon 76437501 76438134 76437501 ID=Msa0959540-mRNA-1:exon:13753;Parent=Msa0959540-mRNA-1
chr6_3 exon 76438158 76439376 76438158 ID=Msa0959540-mRNA-1:exon:13752;Parent=Msa0959540-mRNA-1
chr6_3 exon 76439941 76440014 76439941 ID=Msa0959540-mRNA-1:exon:13751;Parent=Msa0959540-mRNA-1
chr6_3 exon 76441028 76441173 76441028 ID=Msa0959540-mRNA-1:exon:13750;Parent=Msa0959540-mRNA-1
chr6_3 exon 76441281 76441442 76441281 ID=Msa0959540-mRNA-1:exon:13749;Parent=Msa0959540-mRNA-1
chr6_3 CDS 76441281 76441442 76441281 ID=Msa0959540-mRNA-1:cds;Parent=Msa0959540-mRNA-1
chr6_3 CDS 76441028 76441173 76441028 ID=Msa0959540-mRNA-1:cds;Parent=Msa0959540-mRNA-1
chr6_3 CDS 76439941 76440014 76439941 ID=Msa0959540-mRNA-1:cds;Parent=Msa0959540-mRNA-1
chr6_3 CDS 76439222 76439376 76439222 ID=Msa0959540-mRNA-1:cds;Parent=Msa0959540-mRNA-1
chr6_3 three_prime_UTR 76438158 76439221 76438158 ID=Msa0959540-mRNA-1:three_prime_utr;Parent=Msa0959540-mRNA-1
chr6_3 three_prime_UTR 76437501 76438134 76437501 ID=Msa0959540-mRNA-1:three_prime_utr;Parent=Msa0959540-mRNA-1
Gene Sequence

>Msa0959540

ATGGTGGTGACAGTGAGATTAACGGACGCGGTGAAGGGCGATGAGAGCGATTCCGGTGAAGGTGTCTGCGTCGGCAATATAAGCTTCATTCTTCGCTCAATTTCAACATGTCTCTTTGCATGCGCTAGCAGCGGCGAGGGTGGAGGATTGCTGGAGACAATGGGCAGTGAGGGATCAGATCGTCATATTTTGTACGGCTGTCCAGATTCATCAGCCGCAACGGATGAAACCACCGGAATATGTTCAATGTTGACGAGAGACCGGAGAACAACCGAATCTACTGTTGCGACCTCGCTAACGTACCCTAGGTTTATTTCATTGGAGAAGATGCCATCGATGGAGCTTGAGCTTACTGGATATCAATATGTTGGTGGGCCGGCATATGTTGTGCTTAACTATTCAGAGTTGGTTCAAGGTAATTTTCAGCATAATTTCGAGCGAAGTCGTGGCAATTTTCTGCATAATTTAGAGCTGGTTTTCGGCATGTGGACTAATGTAATGCAGCAGCGGTGTTTTGGTACTGCACCTGCTACATAG
Protein sequence

>Msa0959540

MVVTVRLTDAVKGDESDSGEGVCVGNISFILRSISTCLFACASSGEGGGLLETMGSEGSDRHILYGCPDSSAATDETTGICSMLTRDRRTTESTVATSLTYPRFISLEKMPSMELELTGYQYVGGPAYVVLNYSELVQGNFQHNFERSRGNFLHNLELVFGMWTNVMQQRCFGTAPAT*