AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0545540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545540 MtrunA17_Chr4g0026281 98.333 120 2 0 1 120 1 120 9.98e-83 237
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545540 AT2G27730.2 58.197 122 39 5 1 119 1 113 5.35e-30 104
Msa0545540 AT2G27730.3 58.197 122 39 5 1 119 1 113 5.35e-30 104
Msa0545540 AT2G27730.1 58.197 122 39 5 1 119 1 113 5.35e-30 104
Msa0545540 AT2G27730.4 58.197 122 39 5 1 119 1 113 5.35e-30 104

Find 66 sgRNAs with CRISPR-Local

Find 374 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCTTTGCAGTGCCTTTA+AGG 0.112066 4_1:+35935803 None:intergenic
CTTCAATCCTCCTGCACTTC+TGG 0.200917 4_1:+35935781 None:intergenic
GCCTTAGGTTGGTACCTTAA+AGG 0.213610 4_1:-35935817 Msa0545540:CDS
CCTGCAGCTGGTGTTGCTTC+TGG 0.232336 4_1:+35935967 None:intergenic
GTAAAACATTGTAAACAAAA+AGG 0.257575 4_1:+35935641 None:intergenic
AGTGACAAATACCGGAATTA+TGG 0.295334 4_1:-35935871 Msa0545540:CDS
GTGAAGGATACTCAATTATA+AGG 0.339273 4_1:-35935473 Msa0545540:three_prime_UTR
TAAGTGACATTATGAATATA+CGG 0.345784 4_1:+35935610 None:intergenic
AGAGTGAGGGTTCGTTAGAA+AGG 0.370853 4_1:+35941553 None:intergenic
TGTTATCATCTGGTATTCAT+TGG 0.371862 4_1:-35935566 Msa0545540:three_prime_UTR
GCAACACCAGCTGCAGGTTC+AGG 0.372347 4_1:-35935961 Msa0545540:CDS
GCTGGTGTTGCTTCTGGTTG+AGG 0.375661 4_1:+35935973 None:intergenic
GAAAGCTGAGCAAGAGAAGT+TGG 0.381787 4_1:-35939564 Msa0545540:CDS
GTGGCATTTCAACTGAAGTT+AGG 0.388378 4_1:-35935437 Msa0545540:three_prime_UTR
GTATTTGTCACTTGAAACTT+TGG 0.390932 4_1:+35935882 None:intergenic
AGGCTATTTAGCAGTGGCTC+TGG 0.405468 4_1:-35940936 Msa0545540:CDS
CAACACAACACAACACAATC+TGG 0.420954 4_1:-35941530 Msa0545540:intron
ATTCTAAACCCTTCACTTCA+AGG 0.430113 4_1:+35941619 None:intergenic
CTCAAAATTATGTTATCATC+TGG 0.430285 4_1:-35935576 Msa0545540:three_prime_UTR
TATTTGTCACTTGAAACTTT+GGG 0.432133 4_1:+35935883 None:intergenic
CGGAATTATGGAGTCGTAGC+TGG 0.436209 4_1:-35935859 Msa0545540:CDS
CAGCTACGACTCCATAATTC+CGG 0.436283 4_1:+35935860 None:intergenic
ATGTCCAGAGCCACTTGGTT+TGG 0.438617 4_1:+35935921 None:intergenic
TGATTGAGAGAATTTGAGAT+TGG 0.439168 4_1:-35935530 Msa0545540:three_prime_UTR
TGCAAAGAAGCCAGAAGTGC+AGG 0.445155 4_1:-35935791 Msa0545540:CDS
TTCTAAACCCTTCACTTCAA+GGG 0.455383 4_1:+35941620 None:intergenic
TGCGGAAAATGCTTACTTCA+AGG 0.458195 4_1:-35940886 Msa0545540:intron
TTGGTATATGTTGCTTGAGC+TGG 0.475610 4_1:-35935511 Msa0545540:three_prime_UTR
CAGCAAGATTTGCATCTAGA+AGG 0.476156 4_1:-35940956 Msa0545540:CDS
AGTGGCTCTGGGAAAGTTCT+CGG 0.478650 4_1:-35940924 Msa0545540:CDS
GGCTATTTAGCAGTGGCTCT+GGG 0.480604 4_1:-35940935 Msa0545540:CDS
GATGCCAAACCAAGTGGCTC+TGG 0.486204 4_1:-35935925 Msa0545540:CDS
TTTCTACTCAATTATTTGAA+AGG 0.487944 4_1:-35935384 Msa0545540:three_prime_UTR
GTTGGAGAAGCTTGCTCGCA+AGG 0.496581 4_1:-35939546 Msa0545540:intron
TTGTGTTGTGTTGAGAGTGA+GGG 0.506147 4_1:+35941540 None:intergenic
AAGTGACATTATGAATATAC+GGG 0.511357 4_1:+35935611 None:intergenic
TAAACAACCCCTTGAAGTGA+AGG 0.512395 4_1:-35941628 Msa0545540:five_prime_UTR
GTGTTGCTTAAACAAGTTGA+AGG 0.512985 4_1:-35935237 Msa0545540:three_prime_UTR
AATCCATGCTAACAGTATGA+TGG 0.517136 4_1:+35935668 None:intergenic
TCTGGACATTCTGATACATC+AGG 0.522144 4_1:-35935907 Msa0545540:CDS
AAGTTTCAAGTGACAAATAC+CGG 0.523388 4_1:-35935879 Msa0545540:CDS
AGAGCCACTTGGTTTGGCAT+CGG 0.530012 4_1:+35935927 None:intergenic
ATTCCATCATACTGTTAGCA+TGG 0.530985 4_1:-35935671 Msa0545540:three_prime_UTR
AGGATTGAAGATTTCAGTTG+TGG 0.533949 4_1:-35935768 Msa0545540:exon
CATCTGGTATTCATTGGCAA+TGG 0.538596 4_1:-35935560 Msa0545540:three_prime_UTR
GCCTTTAAGGTACCAACCTA+AGG 0.543907 4_1:+35935816 None:intergenic
GTTGTGTTGTGTTGAGAGTG+AGG 0.551002 4_1:+35941539 None:intergenic
CCAGAAGCAACACCAGCTGC+AGG 0.553579 4_1:-35935967 Msa0545540:CDS
AAACAACCCCTTGAAGTGAA+GGG 0.560795 4_1:-35941627 Msa0545540:five_prime_UTR
TCTAAACCCTTCACTTCAAG+GGG 0.564697 4_1:+35941621 None:intergenic
AACTGACAGCAATGGCAACA+CGG 0.581700 4_1:-35940983 Msa0545540:exon
ACTGACAGCAATGGCAACAC+GGG 0.584119 4_1:-35940982 Msa0545540:exon
ATAAGGAAAATCACATTGAG+TGG 0.589683 4_1:-35935456 Msa0545540:three_prime_UTR
TGGGAAAGTTCTCGGTGAGG+AGG 0.595471 4_1:-35940916 Msa0545540:CDS
GAACCACCTGAACCTGCAGC+TGG 0.596837 4_1:+35935955 None:intergenic
TCAGAATGTCCAGAGCCACT+TGG 0.611946 4_1:+35935916 None:intergenic
GCATTGAGTAAGAACTGTGA+AGG 0.622869 4_1:-35935489 Msa0545540:three_prime_UTR
ACACCAGCTGCAGGTTCAGG+TGG 0.626643 4_1:-35935958 Msa0545540:CDS
CTCTGGGAAAGTTCTCGGTG+AGG 0.634132 4_1:-35940919 Msa0545540:CDS
AAAGAAGCCAGAAGTGCAGG+AGG 0.635650 4_1:-35935788 Msa0545540:CDS
GACAGCAATGGCAACACGGG+TGG 0.644840 4_1:-35940979 Msa0545540:exon
TCTAGAAGGCTATTTAGCAG+TGG 0.663582 4_1:-35940942 Msa0545540:CDS
GAAAGGAAGCAAAAGAAGTG+TGG 0.680164 4_1:+35941570 None:intergenic
GTAACCGATGCCAAACCAAG+TGG 0.684341 4_1:-35935931 Msa0545540:CDS
TATGATGGAATAAAGCAGAG+TGG 0.735765 4_1:+35935683 None:intergenic
AGTGACATTATGAATATACG+GGG 0.751726 4_1:+35935612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAACATTAAAAAAAAA+TGG + chr4_1:35935458-35935477 None:intergenic 10.0%
!! AAAATAACAATATCTTAAAA+AGG - chr4_1:35936557-35936576 Msa0545540:intron 10.0%
!! GAAAAATAGATAAAATTAAA+GGG - chr4_1:35937668-35937687 Msa0545540:intron 10.0%
!! AGATTGTTATAAATTATAAA+GGG + chr4_1:35938783-35938802 None:intergenic 10.0%
!! AAATAAAATTGCTAATTATT+TGG + chr4_1:35940482-35940501 None:intergenic 10.0%
!!! TAAAACATGTTTAAAATCAT+TGG - chr4_1:35936501-35936520 Msa0545540:intron 15.0%
!! GGAAAAATAGATAAAATTAA+AGG - chr4_1:35937667-35937686 Msa0545540:intron 15.0%
!!! TTTATTTTTGGGATTTAAAT+GGG + chr4_1:35938180-35938199 None:intergenic 15.0%
!!! TTTTATTTTTGGGATTTAAA+TGG + chr4_1:35938181-35938200 None:intergenic 15.0%
!! ATATTCAATTTCCTAATAAA+CGG + chr4_1:35938282-35938301 None:intergenic 15.0%
!! TAAATTATAAAGGGTAAAAA+GGG + chr4_1:35938774-35938793 None:intergenic 15.0%
!! ATAAATTATAAAGGGTAAAA+AGG + chr4_1:35938775-35938794 None:intergenic 15.0%
!! CAGATTGTTATAAATTATAA+AGG + chr4_1:35938784-35938803 None:intergenic 15.0%
!! AAACTATTTATATTGCTAAA+TGG + chr4_1:35938875-35938894 None:intergenic 15.0%
!!! TATTTTTGGTTTTTAACTTA+TGG - chr4_1:35939117-35939136 Msa0545540:intron 15.0%
!! TAAATGTAAAATGACATAAA+AGG + chr4_1:35939318-35939337 None:intergenic 15.0%
!! TAAATTATAATCATCGTTTA+TGG + chr4_1:35939528-35939547 None:intergenic 15.0%
!! AAAATATAAACTTGAGAAAA+TGG - chr4_1:35940246-35940265 Msa0545540:intron 15.0%
!!! TGTTTTTTTTAATTTCGTTT+TGG - chr4_1:35941093-35941112 Msa0545540:intron 15.0%
!!! GTTTTTTTTAATTTCGTTTT+GGG - chr4_1:35941094-35941113 Msa0545540:intron 15.0%
!! TAAAACATTGTAAACAAAAA+GGG + chr4_1:35941195-35941214 None:intergenic 15.0%
!!! ATGAGTTGTATATTTATTTT+TGG - chr4_1:35941563-35941582 Msa0545540:five_prime_UTR 15.0%
!!! AAAATTCATGTTTTTTGATA+TGG - chr4_1:35941620-35941639 Msa0545540:five_prime_UTR 15.0%
!!! AAATTCATGTTTTTTGATAT+GGG - chr4_1:35941621-35941640 Msa0545540:five_prime_UTR 15.0%
!! TTGAAGTACATTAATAAAAG+TGG - chr4_1:35935670-35935689 Msa0545540:three_prime_UTR 20.0%
!! TGAAGTACATTAATAAAAGT+GGG - chr4_1:35935671-35935690 Msa0545540:three_prime_UTR 20.0%
!!! TTTATTTTCGTAATTGCTAT+CGG - chr4_1:35936020-35936039 Msa0545540:intron 20.0%
!!! TTTTAGAAATTAGAAGGATT+TGG + chr4_1:35936486-35936505 None:intergenic 20.0%
!!! AACATGTTTTAGAAATTAGA+AGG + chr4_1:35936492-35936511 None:intergenic 20.0%
!! ATCTTAAAAAGGAAATGTTA+TGG - chr4_1:35936568-35936587 Msa0545540:intron 20.0%
!! AAAATTTAACTGCATCAAAA+AGG + chr4_1:35936661-35936680 None:intergenic 20.0%
!!! TATTTCAAATGATTTCGTTT+TGG - chr4_1:35936686-35936705 Msa0545540:intron 20.0%
!! ACATGCTTGTATAAATATAA+AGG - chr4_1:35937101-35937120 Msa0545540:intron 20.0%
!!! AAGTTTTTATCAGTTTTTGT+GGG - chr4_1:35937448-35937467 Msa0545540:intron 20.0%
!! ACATTTAGTACTTATTTGAT+GGG + chr4_1:35937728-35937747 None:intergenic 20.0%
!! TACATTTAGTACTTATTTGA+TGG + chr4_1:35937729-35937748 None:intergenic 20.0%
!!! TTGTCATTTTGTTTACTTTT+AGG + chr4_1:35937790-35937809 None:intergenic 20.0%
!!! CTTTTAGAACTTGATTTTAA+TGG + chr4_1:35937814-35937833 None:intergenic 20.0%
!! TCCCAAAAATAAAATACTAA+AGG - chr4_1:35938187-35938206 Msa0545540:intron 20.0%
!!! GCCTTTAGTATTTTATTTTT+GGG + chr4_1:35938191-35938210 None:intergenic 20.0%
!!! TGCCTTTAGTATTTTATTTT+TGG + chr4_1:35938192-35938211 None:intergenic 20.0%
!! TATTCAATTTCCTAATAAAC+GGG + chr4_1:35938281-35938300 None:intergenic 20.0%
!!! ATTATCACTCTTAATCTTTT+GGG + chr4_1:35938451-35938470 None:intergenic 20.0%
!!! TATTATCACTCTTAATCTTT+TGG + chr4_1:35938452-35938471 None:intergenic 20.0%
!! CATATTAAGTATAAGTATGT+GGG + chr4_1:35938497-35938516 None:intergenic 20.0%
!! TCATATTAAGTATAAGTATG+TGG + chr4_1:35938498-35938517 None:intergenic 20.0%
!! AATATGATACAAGATAGATA+TGG - chr4_1:35938511-35938530 Msa0545540:intron 20.0%
!! ATATGATACAAGATAGATAT+GGG - chr4_1:35938512-35938531 Msa0545540:intron 20.0%
!!! GATTTTAGATAAAATAGCAA+GGG + chr4_1:35938752-35938771 None:intergenic 20.0%
!! AAATCATGTGACAAAAAAAT+GGG + chr4_1:35938845-35938864 None:intergenic 20.0%
!! AAAATCATGTGACAAAAAAA+TGG + chr4_1:35938846-35938865 None:intergenic 20.0%
!! GAATATAATAAGTATCCAAT+TGG - chr4_1:35938967-35938986 Msa0545540:intron 20.0%
!!! TCCAATTGGTTTTTATTTTT+TGG - chr4_1:35938981-35939000 Msa0545540:intron 20.0%
!!! CCAATTGGTTTTTATTTTTT+GGG - chr4_1:35938982-35939001 Msa0545540:intron 20.0%
!! CAAAAATATTAAAAGTGAAG+AGG + chr4_1:35939105-35939124 None:intergenic 20.0%
!!! CTCTTCACTTTTAATATTTT+TGG - chr4_1:35939103-35939122 Msa0545540:intron 20.0%
!! ATATAGTTTAATATGTACAG+CGG + chr4_1:35939595-35939614 None:intergenic 20.0%
!!! TACTCTTTTGATATAAATCT+TGG - chr4_1:35939812-35939831 Msa0545540:intron 20.0%
!! CAATGAAATGTATTTAAGTA+AGG - chr4_1:35940179-35940198 Msa0545540:intron 20.0%
!!! AAGAGATATTTTTTAGCAAT+TGG - chr4_1:35940216-35940235 Msa0545540:intron 20.0%
!!! CGTTTTATTATAACCTTAAA+AGG + chr4_1:35940413-35940432 None:intergenic 20.0%
!! GTAAAACATTGTAAACAAAA+AGG + chr4_1:35941196-35941215 None:intergenic 20.0%
!! TAAGTGACATTATGAATATA+CGG + chr4_1:35941227-35941246 None:intergenic 20.0%
!! TTTCTACTCAATTATTTGAA+AGG - chr4_1:35941450-35941469 Msa0545540:intron 20.0%
!! TTTCTCTTTTTCTCTAATGT+TGG - chr4_1:35935330-35935349 Msa0545540:three_prime_UTR 25.0%
!! TTCTCTTTTTCTCTAATGTT+GGG - chr4_1:35935331-35935350 Msa0545540:three_prime_UTR 25.0%
! CAACTCTTAAATACTTAACA+AGG + chr4_1:35935574-35935593 None:intergenic 25.0%
! TAAGTATTTAAGAGTTGAAG+TGG - chr4_1:35935577-35935596 Msa0545540:three_prime_UTR 25.0%
! AAGTATTTAAGAGTTGAAGT+GGG - chr4_1:35935578-35935597 Msa0545540:three_prime_UTR 25.0%
!! TCAAAGTTGATTCAATAAAG+TGG + chr4_1:35935654-35935673 None:intergenic 25.0%
! ATAAATTAAAGCATGCTTTG+AGG + chr4_1:35935977-35935996 None:intergenic 25.0%
!! AATGGTTAAAAAAGTGCATA+TGG - chr4_1:35936392-35936411 Msa0545540:intron 25.0%
! ATTTCTTAGAATTTGTGGTT+GGG + chr4_1:35936931-35936950 None:intergenic 25.0%
! TATTTCTTAGAATTTGTGGT+TGG + chr4_1:35936932-35936951 None:intergenic 25.0%
! GAATAAGAGATGAAATTTGA+GGG + chr4_1:35937318-35937337 None:intergenic 25.0%
! AGAATAAGAGATGAAATTTG+AGG + chr4_1:35937319-35937338 None:intergenic 25.0%
!! GAAGTTTTTATCAGTTTTTG+TGG - chr4_1:35937447-35937466 Msa0545540:intron 25.0%
! TGAGTATTTATGTATCTTGT+TGG + chr4_1:35937642-35937661 None:intergenic 25.0%
! GATACATAAATACTCAAAAG+TGG - chr4_1:35937646-35937665 Msa0545540:intron 25.0%
! GGAACTTATAATTGAAGATA+AGG + chr4_1:35937869-35937888 None:intergenic 25.0%
! CAAGATAATATTGATGTATG+CGG + chr4_1:35937893-35937912 None:intergenic 25.0%
! ATATTATCTTGTTCATCACT+TGG - chr4_1:35937902-35937921 Msa0545540:intron 25.0%
! TGTAAAAATGAGACGATTAA+TGG + chr4_1:35938415-35938434 None:intergenic 25.0%
!!! TTAATCGTCTCATTTTTACA+TGG - chr4_1:35938415-35938434 Msa0545540:intron 25.0%
!! TAAGCTATAAGCTAACTTTT+GGG + chr4_1:35938568-35938587 None:intergenic 25.0%
!! ATAAGCTATAAGCTAACTTT+TGG + chr4_1:35938569-35938588 None:intergenic 25.0%
!! GGATTTTAGATAAAATAGCA+AGG + chr4_1:35938753-35938772 None:intergenic 25.0%
! CCCAAAAAATAAAAACCAAT+TGG + chr4_1:35938985-35939004 None:intergenic 25.0%
!! GATAAGCTAATTAAAGTGTT+TGG + chr4_1:35939366-35939385 None:intergenic 25.0%
! ATAATCATCGTTTATGGATA+AGG + chr4_1:35939522-35939541 None:intergenic 25.0%
! ATTTGCATGATATCTTCATA+GGG + chr4_1:35939623-35939642 None:intergenic 25.0%
! AATTTGCATGATATCTTCAT+AGG + chr4_1:35939624-35939643 None:intergenic 25.0%
! GATTTCCTTAAAAAAACTAG+AGG - chr4_1:35939788-35939807 Msa0545540:intron 25.0%
!!! AGTATCCTCTAGTTTTTTTA+AGG + chr4_1:35939796-35939815 None:intergenic 25.0%
! TATGTATATGCAATCAGATA+TGG - chr4_1:35939842-35939861 Msa0545540:intron 25.0%
! ATGTATATGCAATCAGATAT+GGG - chr4_1:35939843-35939862 Msa0545540:intron 25.0%
!! ATCTAAAATGAAAATGCTTC+AGG + chr4_1:35939935-35939954 None:intergenic 25.0%
! AATAACATGGTTATTGAGAA+AGG + chr4_1:35940125-35940144 None:intergenic 25.0%
! ATCATCTAGTTACAATAACA+TGG + chr4_1:35940138-35940157 None:intergenic 25.0%
! TACTTACAACTCTTAACTAA+AGG - chr4_1:35940455-35940474 Msa0545540:intron 25.0%
! GTCTTTCATTTAGTAGAAAA+AGG + chr4_1:35940523-35940542 None:intergenic 25.0%
! CTACTAAATGAAAGACTTAT+AGG - chr4_1:35940527-35940546 Msa0545540:intron 25.0%
! ATTGACATAAGTTAGAGAAA+CGG + chr4_1:35940585-35940604 None:intergenic 25.0%
! AGCATTTATGTTCTAATGTA+GGG - chr4_1:35940837-35940856 Msa0545540:intron 25.0%
!! TATTTGTCACTTGAAACTTT+GGG + chr4_1:35940954-35940973 None:intergenic 25.0%
! AAGTGACATTATGAATATAC+GGG + chr4_1:35941226-35941245 None:intergenic 25.0%
!! CAGATGATAACATAATTTTG+AGG + chr4_1:35941260-35941279 None:intergenic 25.0%
! CTCAAAATTATGTTATCATC+TGG - chr4_1:35941258-35941277 Msa0545540:intron 25.0%
!!! ATATTTATTTTTGGCTATGC+TGG - chr4_1:35941572-35941591 Msa0545540:five_prime_UTR 25.0%
GTAAAATACGTGTGAATTTC+TGG - chr4_1:35935538-35935557 Msa0545540:three_prime_UTR 30.0%
! TTTTGATGTCAGTGTAAAAC+AGG - chr4_1:35935721-35935740 Msa0545540:three_prime_UTR 30.0%
CTTAAAGAATATACACAGTC+AGG + chr4_1:35935756-35935775 None:intergenic 30.0%
AAGTAAAAAACTGACAGCAA+TGG - chr4_1:35935843-35935862 Msa0545540:CDS 30.0%
!! TGAGGTGTTTTCTTTGTAAT+TGG - chr4_1:35936325-35936344 Msa0545540:intron 30.0%
!! GAGGTGTTTTCTTTGTAATT+GGG - chr4_1:35936326-35936345 Msa0545540:intron 30.0%
! AGGTGTTTTCTTTGTAATTG+GGG - chr4_1:35936327-35936346 Msa0545540:intron 30.0%
ATAAATACCATTGCATGTTC+TGG - chr4_1:35936352-35936371 Msa0545540:intron 30.0%
GATTCTCTTTGTCATTCTAA+TGG - chr4_1:35936374-35936393 Msa0545540:intron 30.0%
!!! AAATGATTTCGTTTTGGCTA+TGG - chr4_1:35936692-35936711 Msa0545540:intron 30.0%
TCCCACATTACTTATAGATA+TGG - chr4_1:35936837-35936856 Msa0545540:intron 30.0%
GCCATATCTATAAGTAATGT+GGG + chr4_1:35936841-35936860 None:intergenic 30.0%
ATTCTTTATGAACACTACAC+AGG + chr4_1:35936863-35936882 None:intergenic 30.0%
TGTGTAGTGTTCATAAAGAA+TGG - chr4_1:35936862-35936881 Msa0545540:intron 30.0%
!! AGGCTATTTCTTAGAATTTG+TGG + chr4_1:35936936-35936955 None:intergenic 30.0%
TTAGATATTAGAAGAACCCA+AGG + chr4_1:35936988-35937007 None:intergenic 30.0%
!!! AATCTTTTTCAATTGCACTG+TGG - chr4_1:35937342-35937361 Msa0545540:intron 30.0%
!! ACTTGAAAGTACTTAACCTT+TGG - chr4_1:35937375-35937394 Msa0545540:intron 30.0%
!! CTTGAAAGTACTTAACCTTT+GGG - chr4_1:35937376-35937395 Msa0545540:intron 30.0%
ACAATGTGTAGGATGATATT+GGG + chr4_1:35937416-35937435 None:intergenic 30.0%
AACAATGTGTAGGATGATAT+TGG + chr4_1:35937417-35937436 None:intergenic 30.0%
TCAATCAGAAAACAATGTGT+AGG + chr4_1:35937427-35937446 None:intergenic 30.0%
! TTTTTATCAGTTTTTGTGGG+TGG - chr4_1:35937451-35937470 Msa0545540:intron 30.0%
! CTATCTAGGATTAACAACAT+AGG + chr4_1:35937516-35937535 None:intergenic 30.0%
ATGTTGTTAATCCTAGATAG+TGG - chr4_1:35937516-35937535 Msa0545540:intron 30.0%
TTTGATGGGAAATGACAAAT+TGG + chr4_1:35937714-35937733 None:intergenic 30.0%
AATTGTGATCTCTTAGTGTT+AGG - chr4_1:35938316-35938335 Msa0545540:intron 30.0%
TGAAGTTTGTACACAGTTAA+CGG - chr4_1:35938341-35938360 Msa0545540:intron 30.0%
!! ACTCTTAATCTTTTGGGAAA+TGG + chr4_1:35938445-35938464 None:intergenic 30.0%
TTGACAAAAAAAGCTCTGTT+TGG - chr4_1:35938604-35938623 Msa0545540:intron 30.0%
AAAAAAGCTCTGTTTGGTAA+AGG - chr4_1:35938610-35938629 Msa0545540:intron 30.0%
ATAAATAAGCTATAAGCTCG+TGG + chr4_1:35938643-35938662 None:intergenic 30.0%
! AAAGTGTTTGGTAAAACTAG+CGG + chr4_1:35939354-35939373 None:intergenic 30.0%
! TTTTTGTAACAATGTACCCA+TGG + chr4_1:35939459-35939478 None:intergenic 30.0%
! ATACATGCTCATTTTGTTAG+TGG + chr4_1:35939662-35939681 None:intergenic 30.0%
TCACAATATGATGAAGAACT+TGG - chr4_1:35939693-35939712 Msa0545540:intron 30.0%
GAATCGTATTCCAAACATAT+GGG - chr4_1:35939760-35939779 Msa0545540:intron 30.0%
AAATCATTGACCCATATGTT+TGG + chr4_1:35939773-35939792 None:intergenic 30.0%
CACATGACAAAAGCAAATTT+CGG + chr4_1:35940052-35940071 None:intergenic 30.0%
!! AAATTTGCTTTTGTCATGTG+TGG - chr4_1:35940052-35940071 Msa0545540:intron 30.0%
TATTGTAACTAGATGATGCT+TGG - chr4_1:35940141-35940160 Msa0545540:intron 30.0%
TATTTAAGTAAGGTGACACA+AGG - chr4_1:35940189-35940208 Msa0545540:intron 30.0%
CATTTAGTAGAAAAAGGAAG+TGG + chr4_1:35940517-35940536 None:intergenic 30.0%
! ATGTGACAAAAAGAAAGTGT+CGG + chr4_1:35940634-35940653 None:intergenic 30.0%
GATAGAAAGAGAAGAGAAAT+GGG + chr4_1:35940730-35940749 None:intergenic 30.0%
AGATAGAAAGAGAAGAGAAA+TGG + chr4_1:35940731-35940750 None:intergenic 30.0%
CATACTACAAGAAATAACAG+TGG + chr4_1:35940780-35940799 None:intergenic 30.0%
GAGCATTTATGTTCTAATGT+AGG - chr4_1:35940836-35940855 Msa0545540:intron 30.0%
! GTATTTGTCACTTGAAACTT+TGG + chr4_1:35940955-35940974 None:intergenic 30.0%
! AAGTTTCAAGTGACAAATAC+CGG - chr4_1:35940955-35940974 Msa0545540:CDS 30.0%
! TGGCACTATAACAATTTTTG+CGG - chr4_1:35940995-35941014 Msa0545540:five_prime_UTR 30.0%
AGTGACATTATGAATATACG+GGG + chr4_1:35941225-35941244 None:intergenic 30.0%
TGTTATCATCTGGTATTCAT+TGG - chr4_1:35941268-35941287 Msa0545540:intron 30.0%
! TGATTGAGAGAATTTGAGAT+TGG - chr4_1:35941304-35941323 Msa0545540:intron 30.0%
GTGAAGGATACTCAATTATA+AGG - chr4_1:35941361-35941380 Msa0545540:intron 30.0%
ATAAGGAAAATCACATTGAG+TGG - chr4_1:35941378-35941397 Msa0545540:intron 30.0%
! ATTTTTGTTACAACAGCTTG+TGG - chr4_1:35941517-35941536 Msa0545540:intron 30.0%
!!! TTCATGTTTTTTGATATGGG+TGG - chr4_1:35941624-35941643 Msa0545540:five_prime_UTR 30.0%
TTCTAAACCCTTCACTTCAA+GGG + chr4_1:35935217-35935236 None:intergenic 35.0%
ATTCTAAACCCTTCACTTCA+AGG + chr4_1:35935218-35935237 None:intergenic 35.0%
CTAGATCTCATCAAGTTTCA+GGG + chr4_1:35935515-35935534 None:intergenic 35.0%
ACTAGATCTCATCAAGTTTC+AGG + chr4_1:35935516-35935535 None:intergenic 35.0%
! AAATACGTGTGAATTTCTGG+TGG - chr4_1:35935541-35935560 Msa0545540:three_prime_UTR 35.0%
GGATGATGTTGCAAAATAGT+AGG - chr4_1:35935692-35935711 Msa0545540:three_prime_UTR 35.0%
!! GGTTGTAGGTTTTGGTATAA+TGG - chr4_1:35936041-35936060 Msa0545540:intron 35.0%
TGCGATACGATCACAAAAAA+CGG + chr4_1:35936080-35936099 None:intergenic 35.0%
CACTATTAATAGTACACCAC+AGG + chr4_1:35936134-35936153 None:intergenic 35.0%
TTCAACGTAATTGCATGTGT+CGG + chr4_1:35936240-35936259 None:intergenic 35.0%
TCAAGTATAACACACACCTT+GGG + chr4_1:35936308-35936327 None:intergenic 35.0%
TTTGGCTATGGCTAATATAC+TGG - chr4_1:35936704-35936723 Msa0545540:intron 35.0%
CTATGGCTAATATACTGGAA+TGG - chr4_1:35936709-35936728 Msa0545540:intron 35.0%
TTCATTGTGTTGGGTTTGAA+GGG - chr4_1:35936804-35936823 Msa0545540:intron 35.0%
GGCCATATCTATAAGTAATG+TGG + chr4_1:35936842-35936861 None:intergenic 35.0%
! ATAGCCTTAGCCAAAGTATT+AGG - chr4_1:35936949-35936968 Msa0545540:intron 35.0%
! TAGCCTTAGCCAAAGTATTA+GGG - chr4_1:35936950-35936969 Msa0545540:intron 35.0%
!! GTCTGTTAGTTTGTTAGTTC+AGG - chr4_1:35937039-35937058 Msa0545540:intron 35.0%
GTTAACAAATGACAACCCAA+AGG + chr4_1:35937394-35937413 None:intergenic 35.0%
TGGGTGGAAAAATGATGAAT+CGG - chr4_1:35937467-35937486 Msa0545540:intron 35.0%
! TATACCGCTATAGCGTTTTT+GGG + chr4_1:35937557-35937576 None:intergenic 35.0%
GATAATATTGATGTATGCGG+TGG + chr4_1:35937890-35937909 None:intergenic 35.0%
TTGGTGTGTTCACATGATAT+AGG - chr4_1:35938141-35938160 Msa0545540:intron 35.0%
TACTAAAGGCAGACACTTTA+TGG - chr4_1:35938201-35938220 Msa0545540:intron 35.0%
ATAAGTACCACCCGTTTATT+AGG - chr4_1:35938268-35938287 Msa0545540:intron 35.0%
TCAATTTCCTAATAAACGGG+TGG + chr4_1:35938278-35938297 None:intergenic 35.0%
!! ATCGTCTCATTTTTACATGG+AGG - chr4_1:35938418-35938437 Msa0545540:intron 35.0%
GATATGGGATGAAGATATGA+AGG - chr4_1:35938527-35938546 Msa0545540:intron 35.0%
TAAAATAGCAAGGGCAAAAG+TGG + chr4_1:35938743-35938762 None:intergenic 35.0%
!!! GCGTTGCTTATTTTGTTTGA+AGG - chr4_1:35939022-35939041 Msa0545540:intron 35.0%
GCTCTGTTTGGTAAAAATAG+CGG + chr4_1:35939423-35939442 None:intergenic 35.0%
! TGCTCATTTTGTTAGTGGTT+CGG + chr4_1:35939657-35939676 None:intergenic 35.0%
GTTCTTCATCATATTGTGAG+AGG + chr4_1:35939692-35939711 None:intergenic 35.0%
CGAATCGTATTCCAAACATA+TGG - chr4_1:35939759-35939778 Msa0545540:intron 35.0%
ACAAAAGCAAATTTCGGCAT+GGG + chr4_1:35940046-35940065 None:intergenic 35.0%
! AAAAGGTATATAACCCCCAA+AGG + chr4_1:35940396-35940415 None:intergenic 35.0%
! TGGGGGTTATATACCTTTTA+AGG - chr4_1:35940397-35940416 Msa0545540:intron 35.0%
!! TGTGACAAAAAGAAAGTGTC+GGG + chr4_1:35940633-35940652 None:intergenic 35.0%
ATGAAATGTGATAAGTGGTG+TGG + chr4_1:35940698-35940717 None:intergenic 35.0%
AGAGCATGAAATGTGATAAG+TGG + chr4_1:35940703-35940722 None:intergenic 35.0%
! TAAAAGTGTGTGATAGTTGC+AGG + chr4_1:35940754-35940773 None:intergenic 35.0%
AATGCTCAACATTAAGCTCA+AGG + chr4_1:35940823-35940842 None:intergenic 35.0%
AGTGACAAATACCGGAATTA+TGG - chr4_1:35940963-35940982 Msa0545540:CDS 35.0%
! ATAACAATTTTTGCGGCCTT+AGG - chr4_1:35941002-35941021 Msa0545540:five_prime_UTR 35.0%
AGGATTGAAGATTTCAGTTG+TGG - chr4_1:35941066-35941085 Msa0545540:intron 35.0%
! TATGATGGAATAAAGCAGAG+TGG + chr4_1:35941154-35941173 None:intergenic 35.0%
ATTCCATCATACTGTTAGCA+TGG - chr4_1:35941163-35941182 Msa0545540:intron 35.0%
AATCCATGCTAACAGTATGA+TGG + chr4_1:35941169-35941188 None:intergenic 35.0%
!! GTGTTGCTTAAACAAGTTGA+AGG - chr4_1:35941597-35941616 Msa0545540:five_prime_UTR 35.0%
AAACAACCCCTTGAAGTGAA+GGG - chr4_1:35935207-35935226 Msa0545540:three_prime_UTR 40.0%
TCTAAACCCTTCACTTCAAG+GGG + chr4_1:35935216-35935235 None:intergenic 40.0%
GAAAGGAAGCAAAAGAAGTG+TGG + chr4_1:35935267-35935286 None:intergenic 40.0%
TTGTGTTGTGTTGAGAGTGA+GGG + chr4_1:35935297-35935316 None:intergenic 40.0%
CAACACAACACAACACAATC+TGG - chr4_1:35935304-35935323 Msa0545540:three_prime_UTR 40.0%
CAGCAAGATTTGCATCTAGA+AGG - chr4_1:35935878-35935897 Msa0545540:CDS 40.0%
TCTAGAAGGCTATTTAGCAG+TGG - chr4_1:35935892-35935911 Msa0545540:CDS 40.0%
TGCGGAAAATGCTTACTTCA+AGG - chr4_1:35935948-35935967 Msa0545540:CDS 40.0%
! TCGTAATTGCTATCGGTTGT+AGG - chr4_1:35936027-35936046 Msa0545540:intron 40.0%
!! TTGCTATCGGTTGTAGGTTT+TGG - chr4_1:35936033-35936052 Msa0545540:intron 40.0%
CACAGGCAAGAAAAATACGA+AGG + chr4_1:35936117-35936136 None:intergenic 40.0%
! CTTCGTATTTTTCTTGCCTG+TGG - chr4_1:35936115-35936134 Msa0545540:intron 40.0%
TGTGTGTATCTTTGTAGTCC+TGG - chr4_1:35936152-35936171 Msa0545540:intron 40.0%
GTTCAGTGTTCAACATGTGT+CGG - chr4_1:35936194-35936213 Msa0545540:intron 40.0%
ATTGACATAGACACAGACAC+TGG + chr4_1:35936281-35936300 None:intergenic 40.0%
CTCAAGTATAACACACACCT+TGG + chr4_1:35936309-35936328 None:intergenic 40.0%
CAAGGTGTGTGTTATACTTG+AGG - chr4_1:35936307-35936326 Msa0545540:intron 40.0%
AGAGAATCCAGAACATGCAA+TGG + chr4_1:35936362-35936381 None:intergenic 40.0%
TGAGCTGTATGTATGATCTC+TGG - chr4_1:35936424-35936443 Msa0545540:intron 40.0%
CTTCAAACCCAACACAATGA+AGG + chr4_1:35936805-35936824 None:intergenic 40.0%
CTTCATTGTGTTGGGTTTGA+AGG - chr4_1:35936803-35936822 Msa0545540:intron 40.0%
TCATTGTGTTGGGTTTGAAG+GGG - chr4_1:35936805-35936824 Msa0545540:intron 40.0%
!!! AAAACCGGCTTTTAAGGTGA+GGG + chr4_1:35936892-35936911 None:intergenic 40.0%
!! ACCTTAAAAGCCGGTTTTGT+AGG - chr4_1:35936894-35936913 Msa0545540:intron 40.0%
!! CCTTAAAAGCCGGTTTTGTA+GGG - chr4_1:35936895-35936914 Msa0545540:intron 40.0%
TGACCCTAATACTTTGGCTA+AGG + chr4_1:35936956-35936975 None:intergenic 40.0%
CACTTGTGACCCTAATACTT+TGG + chr4_1:35936962-35936981 None:intergenic 40.0%
AGAGCAGCAATCTACTTTCT+AGG + chr4_1:35937069-35937088 None:intergenic 40.0%
GTAAGCACCACAAATAGAAC+CGG + chr4_1:35937233-35937252 None:intergenic 40.0%
GGGTGGAAAAATGATGAATC+GGG - chr4_1:35937468-35937487 Msa0545540:intron 40.0%
AAACCCCAAAAACGCTATAG+CGG - chr4_1:35937550-35937569 Msa0545540:intron 40.0%
! ATACCGCTATAGCGTTTTTG+GGG + chr4_1:35937556-35937575 None:intergenic 40.0%
! CTATACCGCTATAGCGTTTT+TGG + chr4_1:35937558-35937577 None:intergenic 40.0%
AAAACGCTATAGCGGTATAG+AGG - chr4_1:35937558-35937577 Msa0545540:intron 40.0%
TATAGCGGTATAGAGGATTG+TGG - chr4_1:35937565-35937584 Msa0545540:intron 40.0%
ATAGCGGTATAGAGGATTGT+GGG - chr4_1:35937566-35937585 Msa0545540:intron 40.0%
AAATGGAGATGAGGAGTTGA+AGG + chr4_1:35937949-35937968 None:intergenic 40.0%
TTCAACTCCTCATCTCCATT+TGG - chr4_1:35937948-35937967 Msa0545540:intron 40.0%
AGATTGCTATAGCGAATAGC+AGG - chr4_1:35937989-35938008 Msa0545540:intron 40.0%
GAAGATATGAAGGCTCTGTT+TGG - chr4_1:35938537-35938556 Msa0545540:intron 40.0%
CTATAAGCTCAAACGCTACT+TGG + chr4_1:35938697-35938716 None:intergenic 40.0%
! GCTCATTTTGTTAGTGGTTC+GGG + chr4_1:35939656-35939675 None:intergenic 40.0%
AGTTCAAACACGTTCTGCTA+TGG - chr4_1:35939997-35940016 Msa0545540:intron 40.0%
AACACGTTCTGCTATGGTTA+TGG - chr4_1:35940003-35940022 Msa0545540:intron 40.0%
GACAAAAGCAAATTTCGGCA+TGG + chr4_1:35940047-35940066 None:intergenic 40.0%
AAATGTGATAAGTGGTGTGG+TGG + chr4_1:35940695-35940714 None:intergenic 40.0%
TCTGGACATTCTGATACATC+AGG - chr4_1:35940927-35940946 Msa0545540:CDS 40.0%
CATCTGGTATTCATTGGCAA+TGG - chr4_1:35941274-35941293 Msa0545540:intron 40.0%
! TTGGTATATGTTGCTTGAGC+TGG - chr4_1:35941323-35941342 Msa0545540:intron 40.0%
GCATTGAGTAAGAACTGTGA+AGG - chr4_1:35941345-35941364 Msa0545540:intron 40.0%
GTGGCATTTCAACTGAAGTT+AGG - chr4_1:35941397-35941416 Msa0545540:intron 40.0%
AGAGTGAGGGTTCGTTAGAA+AGG + chr4_1:35935284-35935303 None:intergenic 45.0%
GTTGTGTTGTGTTGAGAGTG+AGG + chr4_1:35935298-35935317 None:intergenic 45.0%
AACTGACAGCAATGGCAACA+CGG - chr4_1:35935851-35935870 Msa0545540:CDS 45.0%
GTCTATGTCAATGCTTCCCA+AGG - chr4_1:35936289-35936308 Msa0545540:intron 45.0%
AGGCCGCAATTAGGAAAGTA+GGG + chr4_1:35936774-35936793 None:intergenic 45.0%
AAGGCCGCAATTAGGAAAGT+AGG + chr4_1:35936775-35936794 None:intergenic 45.0%
ACTTTCCTAATTGCGGCCTT+CGG - chr4_1:35936775-35936794 Msa0545540:intron 45.0%
ACAACCCTCACCTTAAAAGC+CGG - chr4_1:35936885-35936904 Msa0545540:intron 45.0%
!!! CAAAACCGGCTTTTAAGGTG+AGG + chr4_1:35936893-35936912 None:intergenic 45.0%
!! CCCTACAAAACCGGCTTTTA+AGG + chr4_1:35936898-35936917 None:intergenic 45.0%
CTAACACAGCCCTACAAAAC+CGG + chr4_1:35936907-35936926 None:intergenic 45.0%
CAGCAATCTACTTTCTAGGC+AGG + chr4_1:35937065-35937084 None:intergenic 45.0%
ACAAATAGAACCGGCAAGCA+CGG + chr4_1:35937224-35937243 None:intergenic 45.0%
GAAAGCTGAGCAAGAGAAGT+TGG - chr4_1:35937270-35937289 Msa0545540:intron 45.0%
!!! GCTATAGCGTTTTTGGGGTT+TGG + chr4_1:35937551-35937570 None:intergenic 45.0%
AGTTGTGCCAAATGGAGATG+AGG + chr4_1:35937958-35937977 None:intergenic 45.0%
GTCACTACAGTTGTGCCAAA+TGG + chr4_1:35937966-35937985 None:intergenic 45.0%
! ATAGTGGAATAGGATGGCAG+TGG + chr4_1:35938092-35938111 None:intergenic 45.0%
! ATAGCGCGCTATAGTGGAAT+AGG + chr4_1:35938102-35938121 None:intergenic 45.0%
!! AGCGGTGCTATTGACTACAT+TGG - chr4_1:35938122-35938141 Msa0545540:intron 45.0%
ATATCATCAGACTGCAGCTG+AGG + chr4_1:35938378-35938397 None:intergenic 45.0%
! TCTGTTACTTCGGCTCTGTT+TGG + chr4_1:35939435-35939454 None:intergenic 45.0%
! TACCCATGGCTCTGTTACTT+CGG + chr4_1:35939445-35939464 None:intergenic 45.0%
AGCAGAACGTGTTTGAACTG+TGG + chr4_1:35939996-35940015 None:intergenic 45.0%
GTGGAGATTGCATAACTTCG+TGG - chr4_1:35940071-35940090 Msa0545540:intron 45.0%
AACCACTCTCCTTTGACTGT+TGG - chr4_1:35940338-35940357 Msa0545540:intron 45.0%
TTCCAACAGTCAAAGGAGAG+TGG + chr4_1:35940343-35940362 None:intergenic 45.0%
CTGCAAGTTCCAACAGTCAA+AGG + chr4_1:35940350-35940369 None:intergenic 45.0%
! TCCCTTTTCTTTGCCACCTT+TGG - chr4_1:35940377-35940396 Msa0545540:intron 45.0%
CCCAAAGGTGGCAAAGAAAA+GGG + chr4_1:35940381-35940400 None:intergenic 45.0%
! CCCTTTTCTTTGCCACCTTT+GGG - chr4_1:35940378-35940397 Msa0545540:intron 45.0%
! CCTTTTCTTTGCCACCTTTG+GGG - chr4_1:35940379-35940398 Msa0545540:intron 45.0%
! CTTTTCTTTGCCACCTTTGG+GGG - chr4_1:35940380-35940399 Msa0545540:intron 45.0%
AGGTATATAACCCCCAAAGG+TGG + chr4_1:35940393-35940412 None:intergenic 45.0%
CAGCTACGACTCCATAATTC+CGG + chr4_1:35940977-35940996 None:intergenic 45.0%
! CAATTTTTGCGGCCTTAGGT+TGG - chr4_1:35941006-35941025 Msa0545540:intron 45.0%
!! GCCTTAGGTTGGTACCTTAA+AGG - chr4_1:35941017-35941036 Msa0545540:intron 45.0%
!! GCCTTTAAGGTACCAACCTA+AGG + chr4_1:35941021-35941040 None:intergenic 45.0%
GCTTCTTTGCAGTGCCTTTA+AGG + chr4_1:35941034-35941053 None:intergenic 45.0%
!! ACGTGTGAATTTCTGGTGGC+TGG - chr4_1:35935545-35935564 Msa0545540:three_prime_UTR 50.0%
ACTGACAGCAATGGCAACAC+GGG - chr4_1:35935852-35935871 Msa0545540:CDS 50.0%
AGGCTATTTAGCAGTGGCTC+TGG - chr4_1:35935898-35935917 Msa0545540:CDS 50.0%
GGCTATTTAGCAGTGGCTCT+GGG - chr4_1:35935899-35935918 Msa0545540:CDS 50.0%
!! AGTGGCTCTGGGAAAGTTCT+CGG - chr4_1:35935910-35935929 Msa0545540:CDS 50.0%
GTCCTGGCAACTCTGATCAA+AGG - chr4_1:35936168-35936187 Msa0545540:intron 50.0%
TGCCTTTGATCAGAGTTGCC+AGG + chr4_1:35936173-35936192 None:intergenic 50.0%
TGCATCCTCTGTGCATGTTG+AGG - chr4_1:35936616-35936635 Msa0545540:intron 50.0%
CTTCTCCTCAACATGCACAG+AGG + chr4_1:35936624-35936643 None:intergenic 50.0%
CGCAATTAGGAAAGTAGGGG+CGG + chr4_1:35936770-35936789 None:intergenic 50.0%
CGCCCCTACTTTCCTAATTG+CGG - chr4_1:35936768-35936787 Msa0545540:intron 50.0%
GGCCGCAATTAGGAAAGTAG+GGG + chr4_1:35936773-35936792 None:intergenic 50.0%
CTTTCCTAATTGCGGCCTTC+GGG - chr4_1:35936776-35936795 Msa0545540:intron 50.0%
ACACAATGAAGGCAACCCGA+AGG + chr4_1:35936794-35936813 None:intergenic 50.0%
! TCGGGTTGCCTTCATTGTGT+TGG - chr4_1:35936794-35936813 Msa0545540:intron 50.0%
CGGGTTGCCTTCATTGTGTT+GGG - chr4_1:35936795-35936814 Msa0545540:intron 50.0%
TTGTGTTGGGTTTGAAGGGG+TGG - chr4_1:35936808-35936827 Msa0545540:intron 50.0%
!! CGGTTTTGTAGGGCTGTGTT+AGG - chr4_1:35936905-35936924 Msa0545540:intron 50.0%
! TAGGGTCACAAGTGTTGCCT+TGG - chr4_1:35936968-35936987 Msa0545540:intron 50.0%
! AGGGTCACAAGTGTTGCCTT+GGG - chr4_1:35936969-35936988 Msa0545540:intron 50.0%
!! GTGCTTGCCGGTTCTATTTG+TGG - chr4_1:35937223-35937242 Msa0545540:intron 50.0%
AAGTCATGCTTCCGCATCTG+CGG - chr4_1:35937840-35937859 Msa0545540:intron 50.0%
TTCAGCAGTGGCAGTTGTCA+TGG + chr4_1:35938032-35938051 None:intergenic 50.0%
ATGACAACTGCCACTGCTGA+AGG - chr4_1:35938031-35938050 Msa0545540:intron 50.0%
GGAATAGGATGGCAGTGGTA+GGG + chr4_1:35938087-35938106 None:intergenic 50.0%
TGGAATAGGATGGCAGTGGT+AGG + chr4_1:35938088-35938107 None:intergenic 50.0%
! CGCGCTATAGTGGAATAGGA+TGG + chr4_1:35938098-35938117 None:intergenic 50.0%
TCCACTATAGCGCGCTATAG+CGG - chr4_1:35938104-35938123 Msa0545540:intron 50.0%
AGCCGAAGTAACAGAGCCAT+GGG - chr4_1:35939440-35939459 Msa0545540:intron 50.0%
CCCCAAAGGTGGCAAAGAAA+AGG + chr4_1:35940382-35940401 None:intergenic 50.0%
GTAACCGATGCCAAACCAAG+TGG - chr4_1:35940903-35940922 Msa0545540:CDS 50.0%
!! AGAGCCACTTGGTTTGGCAT+CGG + chr4_1:35940910-35940929 None:intergenic 50.0%
ATGTCCAGAGCCACTTGGTT+TGG + chr4_1:35940916-35940935 None:intergenic 50.0%
TCAGAATGTCCAGAGCCACT+TGG + chr4_1:35940921-35940940 None:intergenic 50.0%
CGGAATTATGGAGTCGTAGC+TGG - chr4_1:35940975-35940994 Msa0545540:CDS 50.0%
! TGCAAAGAAGCCAGAAGTGC+AGG - chr4_1:35941043-35941062 Msa0545540:intron 50.0%
! AAAGAAGCCAGAAGTGCAGG+AGG - chr4_1:35941046-35941065 Msa0545540:intron 50.0%
CTTCAATCCTCCTGCACTTC+TGG + chr4_1:35941056-35941075 None:intergenic 50.0%
! CTCTGGGAAAGTTCTCGGTG+AGG - chr4_1:35935915-35935934 Msa0545540:CDS 55.0%
TGGGAAAGTTCTCGGTGAGG+AGG - chr4_1:35935918-35935937 Msa0545540:CDS 55.0%
CGGTGAGGAGGAAAAAGCTG+CGG - chr4_1:35935930-35935949 Msa0545540:CDS 55.0%
CATGCACAGAGGATGCAGAG+TGG + chr4_1:35936613-35936632 None:intergenic 55.0%
TAGTGAAGCTCCGTGCTTGC+CGG - chr4_1:35937211-35937230 Msa0545540:intron 55.0%
! GTTGGAGAAGCTTGCTCGCA+AGG - chr4_1:35937288-35937307 Msa0545540:intron 55.0%
GGCGCTATGCACCACTATCT+AGG + chr4_1:35937530-35937549 None:intergenic 55.0%
AGATAAGGCAGCCGCAGATG+CGG + chr4_1:35937854-35937873 None:intergenic 55.0%
ACAACTGCCACTGCTGAAGG+TGG - chr4_1:35938034-35938053 Msa0545540:intron 55.0%
! ACTGCTGAAGGTGGCTTGCA+GGG - chr4_1:35938043-35938062 Msa0545540:intron 55.0%
AGGATGGCAGTGGTAGGGTT+TGG + chr4_1:35938082-35938101 None:intergenic 55.0%
ACCGCTATAGCGCGCTATAG+TGG + chr4_1:35938108-35938127 None:intergenic 55.0%
! CACAGTTAACGGATGCCCTC+AGG - chr4_1:35938352-35938371 Msa0545540:intron 55.0%
GAGCCGAAGTAACAGAGCCA+TGG - chr4_1:35939439-35939458 Msa0545540:intron 55.0%
!! GCTGGTGTTGCTTCTGGTTG+AGG + chr4_1:35940864-35940883 None:intergenic 55.0%
GATGCCAAACCAAGTGGCTC+TGG - chr4_1:35940909-35940928 Msa0545540:CDS 55.0%
GACAGCAATGGCAACACGGG+TGG - chr4_1:35935855-35935874 Msa0545540:CDS 60.0%
!! GTAGGGGCGGACTGCAATGA+AGG + chr4_1:35936757-35936776 None:intergenic 60.0%
GCAACCCGAAGGCCGCAATT+AGG + chr4_1:35936783-35936802 None:intergenic 60.0%
CTGCAAGCCACCTTCAGCAG+TGG + chr4_1:35938044-35938063 None:intergenic 60.0%
! CACTGCTGAAGGTGGCTTGC+AGG - chr4_1:35938042-35938061 Msa0545540:intron 60.0%
! GAAGGTGGCTTGCAGGGAGA+TGG - chr4_1:35938049-35938068 Msa0545540:intron 60.0%
AGACTGCAGCTGAGGCCTGA+GGG + chr4_1:35938370-35938389 None:intergenic 60.0%
!! CCTGCAGCTGGTGTTGCTTC+TGG + chr4_1:35940870-35940889 None:intergenic 60.0%
CCAGAAGCAACACCAGCTGC+AGG - chr4_1:35940867-35940886 Msa0545540:intron 60.0%
GCAACACCAGCTGCAGGTTC+AGG - chr4_1:35940873-35940892 Msa0545540:intron 60.0%
ACACCAGCTGCAGGTTCAGG+TGG - chr4_1:35940876-35940895 Msa0545540:intron 60.0%
GAACCACCTGAACCTGCAGC+TGG + chr4_1:35940882-35940901 None:intergenic 60.0%
GGCAGTGGTAGGGTTTGGCG+CGG + chr4_1:35938077-35938096 None:intergenic 65.0%
CAGACTGCAGCTGAGGCCTG+AGG + chr4_1:35938371-35938390 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 35935207 35941649 35935207 ID=Msa0545540;Name=Msa0545540
chr4_1 mRNA 35935207 35941649 35935207 ID=Msa0545540-mRNA-1;Parent=Msa0545540;Name=Msa0545540-mRNA-1;_AED=0.04;_eAED=0.04;_QI=147|1|1|1|0.66|0.5|4|576|120
chr4_1 exon 35935207 35935998 35935207 ID=Msa0545540-mRNA-1:exon:9118;Parent=Msa0545540-mRNA-1
chr4_1 exon 35939547 35939585 35939547 ID=Msa0545540-mRNA-1:exon:9117;Parent=Msa0545540-mRNA-1
chr4_1 exon 35940887 35941022 35940887 ID=Msa0545540-mRNA-1:exon:9116;Parent=Msa0545540-mRNA-1
chr4_1 exon 35941531 35941649 35941531 ID=Msa0545540-mRNA-1:exon:9115;Parent=Msa0545540-mRNA-1
chr4_1 five_prime_UTR 35941531 35941649 35941531 ID=Msa0545540-mRNA-1:five_prime_utr;Parent=Msa0545540-mRNA-1
chr4_1 five_prime_UTR 35940995 35941022 35940995 ID=Msa0545540-mRNA-1:five_prime_utr;Parent=Msa0545540-mRNA-1
chr4_1 CDS 35940887 35940994 35940887 ID=Msa0545540-mRNA-1:cds;Parent=Msa0545540-mRNA-1
chr4_1 CDS 35939547 35939585 35939547 ID=Msa0545540-mRNA-1:cds;Parent=Msa0545540-mRNA-1
chr4_1 CDS 35935783 35935998 35935783 ID=Msa0545540-mRNA-1:cds;Parent=Msa0545540-mRNA-1
chr4_1 three_prime_UTR 35935207 35935782 35935207 ID=Msa0545540-mRNA-1:three_prime_utr;Parent=Msa0545540-mRNA-1
Gene Sequence

>Msa0545540

ATGGCAACACGGGTGGCAGCAAGATTTGCATCTAGAAGGCTATTTAGCAGTGGCTCTGGGAAAGTTCTCGGTGAGGAGGAAAAAGCTGCGGAAAATGCTTACTTCAAGAAAGCTGAGCAAGAGAAGTTGGAGAAGCTTGCTCGCAAGGGTCCTCAACCAGAAGCAACACCAGCTGCAGGTTCAGGTGGTTCAGTAACCGATGCCAAACCAAGTGGCTCTGGACATTCTGATACATCAGGTCCCAAAGTTTCAAGTGACAAATACCGGAATTATGGAGTCGTAGCTGGCACTATAACAATTTTTGCGGCCTTAGGTTGGTACCTTAAAGGCACTGCAAAGAAGCCAGAAGTGCAGGAGGATTGA
Protein sequence

>Msa0545540

MATRVAARFASRRLFSSGSGKVLGEEEKAAENAYFKKAEQEKLEKLARKGPQPEATPAAGSGGSVTDAKPSGSGHSDTSGPKVSSDKYRNYGVVAGTITIFAALGWYLKGTAKKPEVQED*