AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0968880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0968880 MtrunA17_Chr7g0265691 94.030 268 10 1 1 268 1 262 0.0 511
Msa0968880 MtrunA17_Chr7g0265701 83.333 252 41 1 13 264 1 251 2.82e-154 430
Msa0968880 MtrunA17_Chr7g0265581 71.204 191 52 3 72 260 18 207 1.13e-94 278
Msa0968880 MtrunA17_Chr7g0255231 70.068 147 32 5 57 193 76 220 3.26e-61 193
Msa0968880 MtrunA17_Chr7g0219531 40.426 235 127 4 39 266 32 260 5.50e-57 183
Msa0968880 MtrunA17_Chr7g0219541 40.179 224 123 4 48 266 49 266 1.98e-54 177
Msa0968880 MtrunA17_Chr2g0308461 39.381 226 129 3 48 268 281 503 1.91e-50 173
Msa0968880 MtrunA17_Chr7g0219551 38.559 236 131 5 34 264 78 304 7.38e-48 161
Msa0968880 MtrunA17_Chr7g0219521 40.741 189 104 3 33 214 28 215 6.20e-43 145
Msa0968880 MtrunA17_Chr7g0265681 79.592 49 10 0 88 136 25 73 4.41e-23 90.5
Msa0968880 MtrunA17_Chr7g0265671 64.583 48 17 0 213 260 51 98 3.78e-19 80.5
Msa0968880 MtrunA17_Chr7g0265671 72.917 48 10 2 97 141 1 48 3.27e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0968880 AT2G36540.1 48.462 260 111 6 14 265 5 249 1.23e-65 205
Msa0968880 AT3G29760.5 41.500 200 107 3 66 260 321 515 7.29e-46 161
Msa0968880 AT3G29760.3 41.500 200 107 3 66 260 321 515 7.29e-46 161
Msa0968880 AT2G36550.1 51.145 131 60 2 135 265 15 141 8.79e-44 145
Msa0968880 AT4G26190.2 38.710 217 121 5 49 260 843 1052 1.55e-42 155
Msa0968880 AT4G26190.5 38.710 217 121 5 49 260 843 1052 1.55e-42 155
Msa0968880 AT4G26190.3 38.710 217 121 5 49 260 843 1052 1.55e-42 155
Msa0968880 AT4G26190.1 38.710 217 121 5 49 260 843 1052 1.55e-42 155
Msa0968880 AT4G26190.4 38.710 217 121 5 49 260 843 1052 1.55e-42 155
Msa0968880 AT3G29760.2 36.726 226 107 4 66 260 321 541 2.30e-40 147
Msa0968880 AT3G29760.1 42.105 133 68 2 66 193 321 449 9.07e-28 111
Msa0968880 AT3G29760.4 43.836 73 37 1 66 138 321 389 1.60e-12 67.4

Find 72 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTGATTCATTATCTTATT+TGG 0.131323 6_4:+299878 Msa0968880:three_prime_UTR
TTCATAAATTCTTCACTAAA+TGG 0.185254 6_4:-298610 None:intergenic
TGATACACTTTGCAAAATTA+TGG 0.203331 6_4:-298210 None:intergenic
CTCAATTGTTAGCAGTTATA+CGG 0.218202 6_4:-299677 None:intergenic
AAAACAAGCTACTATTTGTT+TGG 0.265573 6_4:+298999 Msa0968880:CDS
ATAATAAGCAATTTCTTCTT+AGG 0.282304 6_4:-298374 None:intergenic
ACAAGTGAATTACCGGATTA+AGG 0.285626 6_4:-299281 None:intergenic
CAAGGTAAATGTAGAGATTC+TGG 0.299926 6_4:+299123 Msa0968880:CDS
TTGCTTATTATGAACCTTAA+CGG 0.337126 6_4:+298386 Msa0968880:CDS
TCAAGAGAAAGACCCAATTC+TGG 0.351025 6_4:-298335 None:intergenic
CCATGGTTCGAATTTGACTT+TGG 0.358354 6_4:+299939 Msa0968880:three_prime_UTR
AAACAAGCTACTATTTGTTT+GGG 0.367088 6_4:+299000 Msa0968880:CDS
TTATGTATTATAAGATCATT+TGG 0.370881 6_4:+299997 Msa0968880:three_prime_UTR
GGTGCTTTAGCCTGTGCTAT+TGG 0.376162 6_4:+298968 Msa0968880:CDS
CATGGTTCGAATTTGACTTT+GGG 0.376887 6_4:+299940 Msa0968880:three_prime_UTR
AGATTCTGGCTTTAAATCCT+TGG 0.379049 6_4:+299137 Msa0968880:CDS
AAGTTCAGCAGAACCAGAAT+TGG 0.383508 6_4:+298322 Msa0968880:CDS
TGTCAGTGACACGAGCTCTA+TGG 0.392097 6_4:-298418 None:intergenic
TAATCAGCGGTCCGAGACTT+TGG 0.398239 6_4:-298452 None:intergenic
TACTTGCATATGTCACCATT+TGG 0.400960 6_4:-299537 None:intergenic
ATACCCATGTAATGATGGTT+TGG 0.409691 6_4:+299838 Msa0968880:three_prime_UTR
TTTGCAAAGTGTATCAAGAA+TGG 0.412015 6_4:+298217 Msa0968880:exon
AAAGTATTTGAAGCAGAATA+TGG 0.421185 6_4:-299228 None:intergenic
GGAACGAACACGAGAATAGA+AGG 0.423928 6_4:-299655 None:intergenic
ATACATTTAATTTAGGATCA+AGG 0.424490 6_4:+299105 Msa0968880:intron
AAAGATTTGAAGTGGGTGTC+TGG 0.432400 6_4:+298644 Msa0968880:CDS
GGATGAGATGTTGTAATTGC+TGG 0.437119 6_4:-299624 None:intergenic
ATACAAGTCTTTCCTTAATC+CGG 0.442145 6_4:+299269 Msa0968880:CDS
TTACAACATCTCATCCTGAT+TGG 0.449737 6_4:+299631 Msa0968880:CDS
GCCTTGTCATCCTTGTCCTC+AGG 0.462508 6_4:-299411 None:intergenic
CGAGAATAGAAGGCCCAATC+AGG 0.463741 6_4:-299645 None:intergenic
TACAACATCTCATCCTGATT+GGG 0.472152 6_4:+299632 Msa0968880:CDS
TGCTTTATAACAAGTGGAAA+AGG 0.475081 6_4:-298157 None:intergenic
TTTACTTGCTTTATAACAAG+TGG 0.489937 6_4:-298163 None:intergenic
GCAATTTATTGTAAAATCCA+TGG 0.492025 6_4:+299922 Msa0968880:three_prime_UTR
GGATTAAGGAAAGACTTGTA+TGG 0.503951 6_4:-299267 None:intergenic
TATGGAGAAGAAATCCGTTA+AGG 0.508168 6_4:-298400 None:intergenic
CTGCTTAGAAAGATTTGAAG+TGG 0.511785 6_4:+298636 Msa0968880:CDS
ATAATTTGAATAGATCCAAA+TGG 0.511910 6_4:+299522 Msa0968880:intron
GGGTGTCTGGTCTTCTGCAA+TGG 0.513381 6_4:+298657 Msa0968880:CDS
AGTTCAGCAGAACCAGAATT+GGG 0.514991 6_4:+298323 Msa0968880:CDS
TTTCGAATCGCCAATAGCAC+AGG 0.520821 6_4:-298978 None:intergenic
AAATGCAGGCATAATGTTGA+TGG 0.521362 6_4:+298947 Msa0968880:intron
TCAATTGTTAGCAGTTATAC+GGG 0.521458 6_4:-299676 None:intergenic
TAATCCAAACCATCATTACA+TGG 0.521997 6_4:-299842 None:intergenic
TCTCTTGAGAAGCTGAATCT+CGG 0.522455 6_4:+298350 Msa0968880:CDS
AAAGGTGAAATAAACCGATT+CGG 0.523055 6_4:-298139 None:intergenic
TGATCATACCCATGTAATGA+TGG 0.530464 6_4:+299833 Msa0968880:three_prime_UTR
GTCCTCAGGATCATATGACT+TGG 0.530501 6_4:-299397 None:intergenic
GTGTGGAATACTGTCAAGAA+AGG 0.534546 6_4:+299201 Msa0968880:CDS
TGCTTAGAAAGATTTGAAGT+GGG 0.535088 6_4:+298637 Msa0968880:CDS
GGACAAGGATGACAAGGCAC+TGG 0.535550 6_4:+299416 Msa0968880:CDS
CACTAAAGGGAACACCGAAT+CGG 0.547540 6_4:+298125 None:intergenic
AGAAAGCAATTCCAAAGTCT+CGG 0.562617 6_4:+298441 Msa0968880:CDS
TCCTCAGGATCATATGACTT+GGG 0.564239 6_4:-299396 None:intergenic
GACATATGCAAGTACTTGAA+AGG 0.564755 6_4:+299546 Msa0968880:CDS
AGGCTTCTTATTGTTCTCCA+AGG 0.569260 6_4:-299154 None:intergenic
TATGTGAAGGATAATGCTAT+TGG 0.576186 6_4:+299597 Msa0968880:CDS
GTCATATGATCCTGAGGACA+AGG 0.576419 6_4:+299401 Msa0968880:CDS
TCCCAAGTCATATGATCCTG+AGG 0.580636 6_4:+299395 Msa0968880:CDS
GACAAGGATGACAAGGCACT+GGG 0.585950 6_4:+299417 Msa0968880:CDS
TTCAATCTATAAAATGTAGA+CGG 0.589902 6_4:-300062 None:intergenic
ATTATGTTACAGTTTACAAG+AGG 0.597781 6_4:+298587 Msa0968880:intron
AATAGCATTATCCTTCACAT+AGG 0.611395 6_4:-299595 None:intergenic
TCCTGAGGACAAGGATGACA+AGG 0.614403 6_4:+299410 Msa0968880:CDS
CCAAAGTCAAATTCGAACCA+TGG 0.623759 6_4:-299939 None:intergenic
AGATGTTCAGTCCTATGTGA+AGG 0.629750 6_4:+299584 Msa0968880:CDS
TGGAATACTGTCAAGAAAGG+AGG 0.665630 6_4:+299204 Msa0968880:CDS
AATCCAAACCATCATTACAT+GGG 0.675720 6_4:-299841 None:intergenic
GTGACGATATTTGTAATCAG+CGG 0.676232 6_4:-298465 None:intergenic
GCTAACAATTGAGTAAAACA+TGG 0.686479 6_4:+299687 Msa0968880:exon
TGTTGCATAATGCAATGCAG+TGG 0.711796 6_4:+299718 Msa0968880:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTATTAT+CGG - chr6_4:298745-298764 None:intergenic 10.0%
!! CAAATTTAAAAAGAAAATAA+GGG - chr6_4:298886-298905 None:intergenic 10.0%
!! TCAAATTTAAAAAGAAAATA+AGG - chr6_4:298887-298906 None:intergenic 10.0%
!!! TCATTTTCTTAAATTATTAT+CGG - chr6_4:298745-298764 None:intergenic 10.0%
!! CAAATTTAAAAAGAAAATAA+GGG - chr6_4:298886-298905 None:intergenic 10.0%
!! TCAAATTTAAAAAGAAAATA+AGG - chr6_4:298887-298906 None:intergenic 10.0%
!!! TTGTTTCATACATTTAATTT+AGG + chr6_4:299098-299117 Msa0968880:intron 15.0%
!!! TCTCTAGAAAAATAAAATAT+AGG - chr6_4:299458-299477 None:intergenic 15.0%
!!! ATTTTATTTTTCTAGAGATT+TGG + chr6_4:299460-299479 Msa0968880:intron 15.0%
!!! TCTAAAATTTTCTTTATTGT+TGG + chr6_4:299805-299824 Msa0968880:three_prime_UTR 15.0%
!! TTATGTATTATAAGATCATT+TGG + chr6_4:299997-300016 Msa0968880:three_prime_UTR 15.0%
!!! TTGTTTCATACATTTAATTT+AGG + chr6_4:299098-299117 Msa0968880:intron 15.0%
!!! TCTCTAGAAAAATAAAATAT+AGG - chr6_4:299458-299477 None:intergenic 15.0%
!!! ATTTTATTTTTCTAGAGATT+TGG + chr6_4:299460-299479 Msa0968880:intron 15.0%
!!! TCTAAAATTTTCTTTATTGT+TGG + chr6_4:299805-299824 Msa0968880:three_prime_UTR 15.0%
!! TTATGTATTATAAGATCATT+TGG + chr6_4:299997-300016 Msa0968880:three_prime_UTR 15.0%
!! ATAATAAGCAATTTCTTCTT+AGG - chr6_4:298377-298396 None:intergenic 20.0%
!! TATATGTGAGAAACTTATTA+CGG - chr6_4:298496-298515 None:intergenic 20.0%
!! TTCATAAATTCTTCACTAAA+TGG - chr6_4:298613-298632 None:intergenic 20.0%
!! ATGAATTCAGTTGTAATTAT+AGG + chr6_4:298761-298780 Msa0968880:intron 20.0%
!!! ACATATCAAGAAATGATTTT+AGG - chr6_4:299053-299072 None:intergenic 20.0%
!!! ATACATTTAATTTAGGATCA+AGG + chr6_4:299105-299124 Msa0968880:intron 20.0%
!! GGAAAAATAATTGATGTATT+GGG - chr6_4:299378-299397 None:intergenic 20.0%
!! ATAATTTGAATAGATCCAAA+TGG + chr6_4:299522-299541 Msa0968880:intron 20.0%
!!! TGGATTAAGTTTCTTTTTAA+AGG + chr6_4:299858-299877 Msa0968880:three_prime_UTR 20.0%
!! TTCAATCTATAAAATGTAGA+CGG - chr6_4:300065-300084 None:intergenic 20.0%
!! ATAATAAGCAATTTCTTCTT+AGG - chr6_4:298377-298396 None:intergenic 20.0%
!! TATATGTGAGAAACTTATTA+CGG - chr6_4:298496-298515 None:intergenic 20.0%
!! TTCATAAATTCTTCACTAAA+TGG - chr6_4:298613-298632 None:intergenic 20.0%
!! ATGAATTCAGTTGTAATTAT+AGG + chr6_4:298761-298780 Msa0968880:intron 20.0%
!!! ACATATCAAGAAATGATTTT+AGG - chr6_4:299053-299072 None:intergenic 20.0%
!!! ATACATTTAATTTAGGATCA+AGG + chr6_4:299105-299124 Msa0968880:intron 20.0%
!! GGAAAAATAATTGATGTATT+GGG - chr6_4:299378-299397 None:intergenic 20.0%
!! ATAATTTGAATAGATCCAAA+TGG + chr6_4:299522-299541 Msa0968880:intron 20.0%
!!! TGGATTAAGTTTCTTTTTAA+AGG + chr6_4:299858-299877 Msa0968880:three_prime_UTR 20.0%
!! TTCAATCTATAAAATGTAGA+CGG - chr6_4:300065-300084 None:intergenic 20.0%
! TTTACTTGCTTTATAACAAG+TGG - chr6_4:298166-298185 None:intergenic 25.0%
! TGATACACTTTGCAAAATTA+TGG - chr6_4:298213-298232 None:intergenic 25.0%
! AAAAAAGAGAGTTGTAGTTA+TGG + chr6_4:298245-298264 Msa0968880:CDS 25.0%
! TTGCTTATTATGAACCTTAA+CGG + chr6_4:298386-298405 Msa0968880:CDS 25.0%
! ATTATGTTACAGTTTACAAG+AGG + chr6_4:298587-298606 Msa0968880:intron 25.0%
! TCTTAAATTATTATCGGTGT+CGG - chr6_4:298739-298758 None:intergenic 25.0%
! AAAACAAGCTACTATTTGTT+TGG + chr6_4:298999-299018 Msa0968880:CDS 25.0%
! AAACAAGCTACTATTTGTTT+GGG + chr6_4:299000-299019 Msa0968880:CDS 25.0%
!! AAAGTATTTGAAGCAGAATA+TGG - chr6_4:299231-299250 None:intergenic 25.0%
! AAGAAAAACAAGTGAATTAC+CGG - chr6_4:299291-299310 None:intergenic 25.0%
! GGGAAAAATAATTGATGTAT+TGG - chr6_4:299379-299398 None:intergenic 25.0%
! AGGTGATTCATTATCTTATT+TGG + chr6_4:299878-299897 Msa0968880:three_prime_UTR 25.0%
! GCAATTTATTGTAAAATCCA+TGG + chr6_4:299922-299941 Msa0968880:three_prime_UTR 25.0%
! TTTACTTGCTTTATAACAAG+TGG - chr6_4:298166-298185 None:intergenic 25.0%
! TGATACACTTTGCAAAATTA+TGG - chr6_4:298213-298232 None:intergenic 25.0%
! AAAAAAGAGAGTTGTAGTTA+TGG + chr6_4:298245-298264 Msa0968880:CDS 25.0%
! TTGCTTATTATGAACCTTAA+CGG + chr6_4:298386-298405 Msa0968880:CDS 25.0%
! ATTATGTTACAGTTTACAAG+AGG + chr6_4:298587-298606 Msa0968880:intron 25.0%
! TCTTAAATTATTATCGGTGT+CGG - chr6_4:298739-298758 None:intergenic 25.0%
! AAAACAAGCTACTATTTGTT+TGG + chr6_4:298999-299018 Msa0968880:CDS 25.0%
! AAACAAGCTACTATTTGTTT+GGG + chr6_4:299000-299019 Msa0968880:CDS 25.0%
!! AAAGTATTTGAAGCAGAATA+TGG - chr6_4:299231-299250 None:intergenic 25.0%
! AAGAAAAACAAGTGAATTAC+CGG - chr6_4:299291-299310 None:intergenic 25.0%
! GGGAAAAATAATTGATGTAT+TGG - chr6_4:299379-299398 None:intergenic 25.0%
! AGGTGATTCATTATCTTATT+TGG + chr6_4:299878-299897 Msa0968880:three_prime_UTR 25.0%
! GCAATTTATTGTAAAATCCA+TGG + chr6_4:299922-299941 Msa0968880:three_prime_UTR 25.0%
AAAGGTGAAATAAACCGATT+CGG - chr6_4:298142-298161 None:intergenic 30.0%
TGCTTTATAACAAGTGGAAA+AGG - chr6_4:298160-298179 None:intergenic 30.0%
TTTGCAAAGTGTATCAAGAA+TGG + chr6_4:298217-298236 Msa0968880:exon 30.0%
! TGCTTAGAAAGATTTGAAGT+GGG + chr6_4:298637-298656 Msa0968880:CDS 30.0%
TCAGTTGTAATTATAGGTGT+CGG + chr6_4:298767-298786 Msa0968880:intron 30.0%
! CTGCATTTAGAGTAACAAAA+GGG - chr6_4:298935-298954 None:intergenic 30.0%
! TTCAGTTCTTTGAAAAAGAG+AGG - chr6_4:299177-299196 None:intergenic 30.0%
TCAAAGAACTGAAAAAAGTG+TGG + chr6_4:299184-299203 Msa0968880:CDS 30.0%
ATACAAGTCTTTCCTTAATC+CGG + chr6_4:299269-299288 Msa0968880:CDS 30.0%
AATAGCATTATCCTTCACAT+AGG - chr6_4:299598-299617 None:intergenic 30.0%
TATGTGAAGGATAATGCTAT+TGG + chr6_4:299597-299616 Msa0968880:CDS 30.0%
TCAATTGTTAGCAGTTATAC+GGG - chr6_4:299679-299698 None:intergenic 30.0%
CTCAATTGTTAGCAGTTATA+CGG - chr6_4:299680-299699 None:intergenic 30.0%
GCTAACAATTGAGTAAAACA+TGG + chr6_4:299687-299706 Msa0968880:exon 30.0%
AATCCAAACCATCATTACAT+GGG - chr6_4:299844-299863 None:intergenic 30.0%
TAATCCAAACCATCATTACA+TGG - chr6_4:299845-299864 None:intergenic 30.0%
AAAGGTGAAATAAACCGATT+CGG - chr6_4:298142-298161 None:intergenic 30.0%
TGCTTTATAACAAGTGGAAA+AGG - chr6_4:298160-298179 None:intergenic 30.0%
TTTGCAAAGTGTATCAAGAA+TGG + chr6_4:298217-298236 Msa0968880:exon 30.0%
! TGCTTAGAAAGATTTGAAGT+GGG + chr6_4:298637-298656 Msa0968880:CDS 30.0%
TCAGTTGTAATTATAGGTGT+CGG + chr6_4:298767-298786 Msa0968880:intron 30.0%
! CTGCATTTAGAGTAACAAAA+GGG - chr6_4:298935-298954 None:intergenic 30.0%
! TTCAGTTCTTTGAAAAAGAG+AGG - chr6_4:299177-299196 None:intergenic 30.0%
TCAAAGAACTGAAAAAAGTG+TGG + chr6_4:299184-299203 Msa0968880:CDS 30.0%
ATACAAGTCTTTCCTTAATC+CGG + chr6_4:299269-299288 Msa0968880:CDS 30.0%
AATAGCATTATCCTTCACAT+AGG - chr6_4:299598-299617 None:intergenic 30.0%
TATGTGAAGGATAATGCTAT+TGG + chr6_4:299597-299616 Msa0968880:CDS 30.0%
TCAATTGTTAGCAGTTATAC+GGG - chr6_4:299679-299698 None:intergenic 30.0%
CTCAATTGTTAGCAGTTATA+CGG - chr6_4:299680-299699 None:intergenic 30.0%
GCTAACAATTGAGTAAAACA+TGG + chr6_4:299687-299706 Msa0968880:exon 30.0%
AATCCAAACCATCATTACAT+GGG - chr6_4:299844-299863 None:intergenic 30.0%
TAATCCAAACCATCATTACA+TGG - chr6_4:299845-299864 None:intergenic 30.0%
! CTGAACTTTTGTGATCATCA+TGG - chr6_4:298310-298329 None:intergenic 35.0%
TATGGAGAAGAAATCCGTTA+AGG - chr6_4:298403-298422 None:intergenic 35.0%
AGAAAGCAATTCCAAAGTCT+CGG + chr6_4:298441-298460 Msa0968880:CDS 35.0%
GTGACGATATTTGTAATCAG+CGG - chr6_4:298468-298487 None:intergenic 35.0%
CTGCTTAGAAAGATTTGAAG+TGG + chr6_4:298636-298655 Msa0968880:CDS 35.0%
! CCTGCATTTAGAGTAACAAA+AGG - chr6_4:298936-298955 None:intergenic 35.0%
! CCTTTTGTTACTCTAAATGC+AGG + chr6_4:298933-298952 Msa0968880:intron 35.0%
AAATGCAGGCATAATGTTGA+TGG + chr6_4:298947-298966 Msa0968880:intron 35.0%
AGCTACTATTTGTTTGGGTA+CGG + chr6_4:299005-299024 Msa0968880:intron 35.0%
CAAGGTAAATGTAGAGATTC+TGG + chr6_4:299123-299142 Msa0968880:CDS 35.0%
!! AGATTCTGGCTTTAAATCCT+TGG + chr6_4:299137-299156 Msa0968880:CDS 35.0%
GGATTAAGGAAAGACTTGTA+TGG - chr6_4:299270-299289 None:intergenic 35.0%
ACAAGTGAATTACCGGATTA+AGG - chr6_4:299284-299303 None:intergenic 35.0%
TACTTGCATATGTCACCATT+TGG - chr6_4:299540-299559 None:intergenic 35.0%
GACATATGCAAGTACTTGAA+AGG + chr6_4:299546-299565 Msa0968880:CDS 35.0%
TTACAACATCTCATCCTGAT+TGG + chr6_4:299631-299650 Msa0968880:CDS 35.0%
TACAACATCTCATCCTGATT+GGG + chr6_4:299632-299651 Msa0968880:CDS 35.0%
TGATCATACCCATGTAATGA+TGG + chr6_4:299833-299852 Msa0968880:three_prime_UTR 35.0%
ATACCCATGTAATGATGGTT+TGG + chr6_4:299838-299857 Msa0968880:three_prime_UTR 35.0%
! CATGGTTCGAATTTGACTTT+GGG + chr6_4:299940-299959 Msa0968880:three_prime_UTR 35.0%
! CTGAACTTTTGTGATCATCA+TGG - chr6_4:298310-298329 None:intergenic 35.0%
TATGGAGAAGAAATCCGTTA+AGG - chr6_4:298403-298422 None:intergenic 35.0%
AGAAAGCAATTCCAAAGTCT+CGG + chr6_4:298441-298460 Msa0968880:CDS 35.0%
GTGACGATATTTGTAATCAG+CGG - chr6_4:298468-298487 None:intergenic 35.0%
CTGCTTAGAAAGATTTGAAG+TGG + chr6_4:298636-298655 Msa0968880:CDS 35.0%
! CCTGCATTTAGAGTAACAAA+AGG - chr6_4:298936-298955 None:intergenic 35.0%
! CCTTTTGTTACTCTAAATGC+AGG + chr6_4:298933-298952 Msa0968880:intron 35.0%
AAATGCAGGCATAATGTTGA+TGG + chr6_4:298947-298966 Msa0968880:intron 35.0%
AGCTACTATTTGTTTGGGTA+CGG + chr6_4:299005-299024 Msa0968880:intron 35.0%
CAAGGTAAATGTAGAGATTC+TGG + chr6_4:299123-299142 Msa0968880:CDS 35.0%
!! AGATTCTGGCTTTAAATCCT+TGG + chr6_4:299137-299156 Msa0968880:CDS 35.0%
GGATTAAGGAAAGACTTGTA+TGG - chr6_4:299270-299289 None:intergenic 35.0%
ACAAGTGAATTACCGGATTA+AGG - chr6_4:299284-299303 None:intergenic 35.0%
TACTTGCATATGTCACCATT+TGG - chr6_4:299540-299559 None:intergenic 35.0%
GACATATGCAAGTACTTGAA+AGG + chr6_4:299546-299565 Msa0968880:CDS 35.0%
TTACAACATCTCATCCTGAT+TGG + chr6_4:299631-299650 Msa0968880:CDS 35.0%
TACAACATCTCATCCTGATT+GGG + chr6_4:299632-299651 Msa0968880:CDS 35.0%
TGATCATACCCATGTAATGA+TGG + chr6_4:299833-299852 Msa0968880:three_prime_UTR 35.0%
ATACCCATGTAATGATGGTT+TGG + chr6_4:299838-299857 Msa0968880:three_prime_UTR 35.0%
! CATGGTTCGAATTTGACTTT+GGG + chr6_4:299940-299959 Msa0968880:three_prime_UTR 35.0%
AAGTTCAGCAGAACCAGAAT+TGG + chr6_4:298322-298341 Msa0968880:CDS 40.0%
AGTTCAGCAGAACCAGAATT+GGG + chr6_4:298323-298342 Msa0968880:CDS 40.0%
TCAAGAGAAAGACCCAATTC+TGG - chr6_4:298338-298357 None:intergenic 40.0%
TCTCTTGAGAAGCTGAATCT+CGG + chr6_4:298350-298369 Msa0968880:CDS 40.0%
! AAAGATTTGAAGTGGGTGTC+TGG + chr6_4:298644-298663 Msa0968880:CDS 40.0%
AACACTCTTCCTATGAAGCA+CGG + chr6_4:298691-298710 Msa0968880:intron 40.0%
!! GTAATTATAGGTGTCGGTGT+CGG + chr6_4:298773-298792 Msa0968880:intron 40.0%
AGGCTTCTTATTGTTCTCCA+AGG - chr6_4:299157-299176 None:intergenic 40.0%
GTGTGGAATACTGTCAAGAA+AGG + chr6_4:299201-299220 Msa0968880:CDS 40.0%
TGGAATACTGTCAAGAAAGG+AGG + chr6_4:299204-299223 Msa0968880:CDS 40.0%
ATGTATTGGGCTGCAATTTG+AGG - chr6_4:299365-299384 None:intergenic 40.0%
TCCTCAGGATCATATGACTT+GGG - chr6_4:299399-299418 None:intergenic 40.0%
AGATGTTCAGTCCTATGTGA+AGG + chr6_4:299584-299603 Msa0968880:CDS 40.0%
GGATGAGATGTTGTAATTGC+TGG - chr6_4:299627-299646 None:intergenic 40.0%
TGTTGCATAATGCAATGCAG+TGG + chr6_4:299718-299737 Msa0968880:three_prime_UTR 40.0%
CCAAAGTCAAATTCGAACCA+TGG - chr6_4:299942-299961 None:intergenic 40.0%
! CCATGGTTCGAATTTGACTT+TGG + chr6_4:299939-299958 Msa0968880:three_prime_UTR 40.0%
AAGTTCAGCAGAACCAGAAT+TGG + chr6_4:298322-298341 Msa0968880:CDS 40.0%
AGTTCAGCAGAACCAGAATT+GGG + chr6_4:298323-298342 Msa0968880:CDS 40.0%
TCAAGAGAAAGACCCAATTC+TGG - chr6_4:298338-298357 None:intergenic 40.0%
TCTCTTGAGAAGCTGAATCT+CGG + chr6_4:298350-298369 Msa0968880:CDS 40.0%
! AAAGATTTGAAGTGGGTGTC+TGG + chr6_4:298644-298663 Msa0968880:CDS 40.0%
AACACTCTTCCTATGAAGCA+CGG + chr6_4:298691-298710 Msa0968880:intron 40.0%
!! GTAATTATAGGTGTCGGTGT+CGG + chr6_4:298773-298792 Msa0968880:intron 40.0%
AGGCTTCTTATTGTTCTCCA+AGG - chr6_4:299157-299176 None:intergenic 40.0%
GTGTGGAATACTGTCAAGAA+AGG + chr6_4:299201-299220 Msa0968880:CDS 40.0%
TGGAATACTGTCAAGAAAGG+AGG + chr6_4:299204-299223 Msa0968880:CDS 40.0%
ATGTATTGGGCTGCAATTTG+AGG - chr6_4:299365-299384 None:intergenic 40.0%
TCCTCAGGATCATATGACTT+GGG - chr6_4:299399-299418 None:intergenic 40.0%
AGATGTTCAGTCCTATGTGA+AGG + chr6_4:299584-299603 Msa0968880:CDS 40.0%
GGATGAGATGTTGTAATTGC+TGG - chr6_4:299627-299646 None:intergenic 40.0%
TGTTGCATAATGCAATGCAG+TGG + chr6_4:299718-299737 Msa0968880:three_prime_UTR 40.0%
CCAAAGTCAAATTCGAACCA+TGG - chr6_4:299942-299961 None:intergenic 40.0%
! CCATGGTTCGAATTTGACTT+TGG + chr6_4:299939-299958 Msa0968880:three_prime_UTR 40.0%
TATGAAGCACGAACACTCCT+CGG - chr6_4:298835-298854 None:intergenic 45.0%
TTTCGAATCGCCAATAGCAC+AGG - chr6_4:298981-299000 None:intergenic 45.0%
TCCCAAGTCATATGATCCTG+AGG + chr6_4:299395-299414 Msa0968880:CDS 45.0%
GTCCTCAGGATCATATGACT+TGG - chr6_4:299400-299419 None:intergenic 45.0%
GTCATATGATCCTGAGGACA+AGG + chr6_4:299401-299420 Msa0968880:CDS 45.0%
GGAACGAACACGAGAATAGA+AGG - chr6_4:299658-299677 None:intergenic 45.0%
TATGAAGCACGAACACTCCT+CGG - chr6_4:298835-298854 None:intergenic 45.0%
TTTCGAATCGCCAATAGCAC+AGG - chr6_4:298981-299000 None:intergenic 45.0%
TCCCAAGTCATATGATCCTG+AGG + chr6_4:299395-299414 Msa0968880:CDS 45.0%
GTCCTCAGGATCATATGACT+TGG - chr6_4:299400-299419 None:intergenic 45.0%
GTCATATGATCCTGAGGACA+AGG + chr6_4:299401-299420 Msa0968880:CDS 45.0%
GGAACGAACACGAGAATAGA+AGG - chr6_4:299658-299677 None:intergenic 45.0%
TGTCAGTGACACGAGCTCTA+TGG - chr6_4:298421-298440 None:intergenic 50.0%
TAATCAGCGGTCCGAGACTT+TGG - chr6_4:298455-298474 None:intergenic 50.0%
TATGAAGCACGGACACGACA+CGG + chr6_4:298702-298721 Msa0968880:intron 50.0%
!! TATTATCGGTGTCGGCGTGT+GGG - chr6_4:298731-298750 None:intergenic 50.0%
!! TTATTATCGGTGTCGGCGTG+TGG - chr6_4:298732-298751 None:intergenic 50.0%
GATTAGGTATGTCCCGGTGT+CGG - chr6_4:298813-298832 None:intergenic 50.0%
! TCCTCGGATTAGGTATGTCC+CGG - chr6_4:298819-298838 None:intergenic 50.0%
GGTGCTTTAGCCTGTGCTAT+TGG + chr6_4:298968-298987 Msa0968880:CDS 50.0%
TCCTGAGGACAAGGATGACA+AGG + chr6_4:299410-299429 Msa0968880:CDS 50.0%
! GACAAGGATGACAAGGCACT+GGG + chr6_4:299417-299436 Msa0968880:CDS 50.0%
CGAGAATAGAAGGCCCAATC+AGG - chr6_4:299648-299667 None:intergenic 50.0%
TGTCAGTGACACGAGCTCTA+TGG - chr6_4:298421-298440 None:intergenic 50.0%
TAATCAGCGGTCCGAGACTT+TGG - chr6_4:298455-298474 None:intergenic 50.0%
TATGAAGCACGGACACGACA+CGG + chr6_4:298702-298721 Msa0968880:intron 50.0%
!! TATTATCGGTGTCGGCGTGT+GGG - chr6_4:298731-298750 None:intergenic 50.0%
!! TTATTATCGGTGTCGGCGTG+TGG - chr6_4:298732-298751 None:intergenic 50.0%
GATTAGGTATGTCCCGGTGT+CGG - chr6_4:298813-298832 None:intergenic 50.0%
! TCCTCGGATTAGGTATGTCC+CGG - chr6_4:298819-298838 None:intergenic 50.0%
GGTGCTTTAGCCTGTGCTAT+TGG + chr6_4:298968-298987 Msa0968880:CDS 50.0%
TCCTGAGGACAAGGATGACA+AGG + chr6_4:299410-299429 Msa0968880:CDS 50.0%
! GACAAGGATGACAAGGCACT+GGG + chr6_4:299417-299436 Msa0968880:CDS 50.0%
CGAGAATAGAAGGCCCAATC+AGG - chr6_4:299648-299667 None:intergenic 50.0%
! GGGTGTCTGGTCTTCTGCAA+TGG + chr6_4:298657-298676 Msa0968880:CDS 55.0%
GTGTCGTGTCCGTGCTTCAT+AGG - chr6_4:298703-298722 None:intergenic 55.0%
ACCGGGACATACCTAATCCG+AGG + chr6_4:298815-298834 Msa0968880:intron 55.0%
GCACGAACACTCCTCGGATT+AGG - chr6_4:298829-298848 None:intergenic 55.0%
GCCTTGTCATCCTTGTCCTC+AGG - chr6_4:299414-299433 None:intergenic 55.0%
GGACAAGGATGACAAGGCAC+TGG + chr6_4:299416-299435 Msa0968880:CDS 55.0%
! GGGTGTCTGGTCTTCTGCAA+TGG + chr6_4:298657-298676 Msa0968880:CDS 55.0%
GTGTCGTGTCCGTGCTTCAT+AGG - chr6_4:298703-298722 None:intergenic 55.0%
ACCGGGACATACCTAATCCG+AGG + chr6_4:298815-298834 Msa0968880:intron 55.0%
GCACGAACACTCCTCGGATT+AGG - chr6_4:298829-298848 None:intergenic 55.0%
GCCTTGTCATCCTTGTCCTC+AGG - chr6_4:299414-299433 None:intergenic 55.0%
GGACAAGGATGACAAGGCAC+TGG + chr6_4:299416-299435 Msa0968880:CDS 55.0%
!! CACCGACTCGTATCCGACAC+CGG + chr6_4:298797-298816 Msa0968880:intron 60.0%
!! ACCGACTCGTATCCGACACC+GGG + chr6_4:298798-298817 Msa0968880:intron 60.0%
! TCCCGGTGTCGGATACGAGT+CGG - chr6_4:298802-298821 None:intergenic 60.0%
!! CACCGACTCGTATCCGACAC+CGG + chr6_4:298797-298816 Msa0968880:intron 60.0%
!! ACCGACTCGTATCCGACACC+GGG + chr6_4:298798-298817 Msa0968880:intron 60.0%
! TCCCGGTGTCGGATACGAGT+CGG - chr6_4:298802-298821 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 298139 300095 298139 ID=Msa0968880;Name=Msa0968880
chr6_4 mRNA 298139 300095 298139 ID=Msa0968880-mRNA-1;Parent=Msa0968880;Name=Msa0968880-mRNA-1;_AED=0.08;_eAED=0.08;_QI=97|1|1|1|1|1|6|397|268
chr6_4 exon 298139 298494 298139 ID=Msa0968880-mRNA-1:exon:197;Parent=Msa0968880-mRNA-1
chr6_4 exon 298599 298680 298599 ID=Msa0968880-mRNA-1:exon:198;Parent=Msa0968880-mRNA-1
chr6_4 exon 298955 299021 298955 ID=Msa0968880-mRNA-1:exon:199;Parent=Msa0968880-mRNA-1
chr6_4 exon 299120 299290 299120 ID=Msa0968880-mRNA-1:exon:200;Parent=Msa0968880-mRNA-1
chr6_4 exon 299375 299438 299375 ID=Msa0968880-mRNA-1:exon:201;Parent=Msa0968880-mRNA-1
chr6_4 exon 299535 300095 299535 ID=Msa0968880-mRNA-1:exon:202;Parent=Msa0968880-mRNA-1
chr6_4 five_prime_UTR 298139 298235 298139 ID=Msa0968880-mRNA-1:five_prime_utr;Parent=Msa0968880-mRNA-1
chr6_4 CDS 298236 298494 298236 ID=Msa0968880-mRNA-1:cds;Parent=Msa0968880-mRNA-1
chr6_4 CDS 298599 298680 298599 ID=Msa0968880-mRNA-1:cds;Parent=Msa0968880-mRNA-1
chr6_4 CDS 298955 299021 298955 ID=Msa0968880-mRNA-1:cds;Parent=Msa0968880-mRNA-1
chr6_4 CDS 299120 299290 299120 ID=Msa0968880-mRNA-1:cds;Parent=Msa0968880-mRNA-1
chr6_4 CDS 299375 299438 299375 ID=Msa0968880-mRNA-1:cds;Parent=Msa0968880-mRNA-1
chr6_4 CDS 299535 299698 299535 ID=Msa0968880-mRNA-1:cds;Parent=Msa0968880-mRNA-1
chr6_4 three_prime_UTR 299699 300095 299699 ID=Msa0968880-mRNA-1:three_prime_utr;Parent=Msa0968880-mRNA-1
Gene Sequence

>Msa0968880

ATGGAAGCAAAAAAAGAGAGTTGTAGTTATGGAAATATCAAAATCAAAGACTCTGAAGCTGATCATGATGACCATGATGATCACAAAAGTTCAGCAGAACCAGAATTGGGTCTTTCTCTTGAGAAGCTGAATCTCGGACCTAAGAAGAAATTGCTTATTATGAACCTTAACGGATTTCTTCTCCATAGAGCTCGTGTCACTGACAAGAAAGCAATTCCAAAGTCTCGGACCGCTGATTACAAATATCGTCACTTTCTCCTTTACAAGAGGCCATTTAGTGAAGAATTTATGAAATTCTGCTTAGAAAGATTTGAAGTGGGTGTCTGGTCTTCTGCAATGGAGCATAATGTTGATGGTGCTTTAGCCTGTGCTATTGGCGATTCGAAAAACAAGCTACTATTTGTTTGGGATCAAGGTAAATGTAGAGATTCTGGCTTTAAATCCTTGGAGAACAATAAGAAGCCTCTCTTTTTCAAAGAACTGAAAAAAGTGTGGAATACTGTCAAGAAAGGAGGACCATATTCTGCTTCAAATACTTTGCTGATCGATGACAAACCATACAAGTCTTTCCTTAATCCGCCCAATACATCAATTATTTTTCCCAAGTCATATGATCCTGAGGACAAGGATGACAAGGCACTGGATCCAAATGGTGACATATGCAAGTACTTGAAAGGAGTAGCTGAAGCTGAAGATGTTCAGTCCTATGTGAAGGATAATGCTATTGGTGAACCAGCAATTACAACATCTCATCCTGATTGGGCCTTCTATTCTCGTGTTCGTTCCCGTATAACTGCTAACAATTGA
Protein sequence

>Msa0968880

MEAKKESCSYGNIKIKDSEADHDDHDDHKSSAEPELGLSLEKLNLGPKKKLLIMNLNGFLLHRARVTDKKAIPKSRTADYKYRHFLLYKRPFSEEFMKFCLERFEVGVWSSAMEHNVDGALACAIGDSKNKLLFVWDQGKCRDSGFKSLENNKKPLFFKELKKVWNTVKKGGPYSASNTLLIDDKPYKSFLNPPNTSIIFPKSYDPEDKDDKALDPNGDICKYLKGVAEAEDVQSYVKDNAIGEPAITTSHPDWAFYSRVRSRITANN*