AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1423100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1423100 MtrunA17_Chr7g0265691 94.776 268 8 1 1 268 1 262 0.0 513
Msa1423100 MtrunA17_Chr7g0265701 82.937 252 42 1 13 264 1 251 1.20e-153 428
Msa1423100 MtrunA17_Chr7g0265581 70.681 191 53 3 72 260 18 207 9.49e-95 278
Msa1423100 MtrunA17_Chr7g0255231 68.707 147 34 5 57 193 76 220 3.14e-60 190
Msa1423100 MtrunA17_Chr7g0219531 40.426 235 127 4 39 266 32 260 1.93e-57 184
Msa1423100 MtrunA17_Chr7g0219541 39.732 224 124 4 48 266 49 266 8.38e-54 175
Msa1423100 MtrunA17_Chr2g0308461 39.381 226 129 3 48 268 281 503 7.00e-51 174
Msa1423100 MtrunA17_Chr7g0219551 38.559 236 131 5 34 264 78 304 2.61e-48 162
Msa1423100 MtrunA17_Chr7g0219521 40.741 189 104 3 33 214 28 215 1.92e-43 147
Msa1423100 MtrunA17_Chr7g0265681 79.592 49 10 0 88 136 25 73 4.51e-23 90.1
Msa1423100 MtrunA17_Chr7g0265671 64.583 48 17 0 213 260 51 98 3.91e-19 80.5
Msa1423100 MtrunA17_Chr7g0265671 72.917 48 10 2 97 141 1 48 3.48e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1423100 AT2G36540.1 48.462 260 111 6 14 265 5 249 1.45e-65 205
Msa1423100 AT3G29760.5 41.500 200 107 3 66 260 321 515 3.57e-46 162
Msa1423100 AT3G29760.3 41.500 200 107 3 66 260 321 515 3.57e-46 162
Msa1423100 AT2G36550.1 51.145 131 60 2 135 265 15 141 9.38e-44 145
Msa1423100 AT4G26190.2 38.710 217 121 4 49 260 843 1052 2.62e-43 157
Msa1423100 AT4G26190.5 38.710 217 121 4 49 260 843 1052 2.62e-43 157
Msa1423100 AT4G26190.3 38.710 217 121 4 49 260 843 1052 2.62e-43 157
Msa1423100 AT4G26190.1 38.710 217 121 4 49 260 843 1052 2.62e-43 157
Msa1423100 AT4G26190.4 38.710 217 121 4 49 260 843 1052 2.62e-43 157
Msa1423100 AT3G29760.2 36.726 226 107 4 66 260 321 541 1.13e-40 147
Msa1423100 AT3G29760.1 42.105 133 68 2 66 193 321 449 4.21e-28 112
Msa1423100 AT3G29760.4 43.836 73 37 1 66 138 321 389 1.71e-12 67.4

Find 71 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTGATTCATTATCTTATT+TGG 0.131323 tig0025511:+69480 Msa1423100:three_prime_UTR
TTCATAAATTCTTCACTAAA+TGG 0.185254 tig0025511:-68208 None:intergenic
TGATACACTTTGCAAAATTA+TGG 0.203331 tig0025511:-67808 None:intergenic
CTCAATTGTTAGCAGTTATA+CGG 0.218202 tig0025511:-69279 None:intergenic
AAAACAAGCTACTATTTGTT+TGG 0.265573 tig0025511:+68601 Msa1423100:CDS
ATAATAAGCAATTTCTTCTT+AGG 0.282304 tig0025511:-67972 None:intergenic
ACAAGTGAATTACCGGATTA+AGG 0.285626 tig0025511:-68883 None:intergenic
CAAGGTAAATGTAGAGATTC+TGG 0.299926 tig0025511:+68725 Msa1423100:CDS
TTGCTTATTATGAACCTTAA+CGG 0.337126 tig0025511:+67984 Msa1423100:CDS
TCAAGAGAAAGACCCAATTC+TGG 0.351025 tig0025511:-67933 None:intergenic
CCATGGTTCGAATTTGACTT+TGG 0.358354 tig0025511:+69541 Msa1423100:three_prime_UTR
AAACAAGCTACTATTTGTTT+GGG 0.367088 tig0025511:+68602 Msa1423100:CDS
TTATGTATTATAAGATCATT+TGG 0.370881 tig0025511:+69599 Msa1423100:three_prime_UTR
GGTGCTTTAGCCTGTGCTAT+TGG 0.376162 tig0025511:+68570 Msa1423100:CDS
CATGGTTCGAATTTGACTTT+GGG 0.376887 tig0025511:+69542 Msa1423100:three_prime_UTR
AGATTCTGGCTTTAAATCCT+TGG 0.379049 tig0025511:+68739 Msa1423100:CDS
AAGTTCAGCAGAACCAGAAT+TGG 0.383508 tig0025511:+67920 Msa1423100:CDS
TGTCAGTGACACGAGCTCTA+TGG 0.392097 tig0025511:-68016 None:intergenic
TAATCAGCGGTCCGAGACTT+TGG 0.398239 tig0025511:-68050 None:intergenic
TACTTGCATATGTCACCATT+TGG 0.400960 tig0025511:-69139 None:intergenic
ATACCCATGTAATGATGGTT+TGG 0.409691 tig0025511:+69440 Msa1423100:three_prime_UTR
TTTGCAAAGTGTATCAAGAA+TGG 0.412015 tig0025511:+67815 Msa1423100:exon
AAAGTATTTGAAGCAGAATA+TGG 0.421185 tig0025511:-68830 None:intergenic
GGAACGAACACGAGAATAGA+AGG 0.423928 tig0025511:-69257 None:intergenic
ATACATTTAATTTAGGATCA+AGG 0.424490 tig0025511:+68707 Msa1423100:intron
AAAGATTTGAAGTGGGTGTC+TGG 0.432400 tig0025511:+68242 Msa1423100:CDS
GGATGAGATGTTGTAATTGC+TGG 0.437119 tig0025511:-69226 None:intergenic
ATACAAGTCTTTCCTTAATC+CGG 0.442145 tig0025511:+68871 Msa1423100:CDS
TTACAACATCTCATCCTGAT+TGG 0.449737 tig0025511:+69233 Msa1423100:CDS
GCCTTGTCATCCTTGTCCTC+AGG 0.462508 tig0025511:-69013 None:intergenic
CGAGAATAGAAGGCCCAATC+AGG 0.463741 tig0025511:-69247 None:intergenic
TACAACATCTCATCCTGATT+GGG 0.472152 tig0025511:+69234 Msa1423100:CDS
TGCTTTATAACAAGTGGAAA+AGG 0.475081 tig0025511:-67755 None:intergenic
TTTACTTGCTTTATAACAAG+TGG 0.489937 tig0025511:-67761 None:intergenic
GCAATTTATTGTAAAATCCA+TGG 0.492025 tig0025511:+69524 Msa1423100:three_prime_UTR
GGATTAAGGAAAGACTTGTA+TGG 0.503951 tig0025511:-68869 None:intergenic
TATGGAGAAGAAATCCGTTA+AGG 0.508168 tig0025511:-67998 None:intergenic
CTGCTTAGAAAGATTTGAAG+TGG 0.511785 tig0025511:+68234 Msa1423100:CDS
ATAATTTGAATAGATCCAAA+TGG 0.511910 tig0025511:+69124 Msa1423100:intron
GGGTGTCTGGTCTTCTGCAA+TGG 0.513381 tig0025511:+68255 Msa1423100:CDS
AGTTCAGCAGAACCAGAATT+GGG 0.514991 tig0025511:+67921 Msa1423100:CDS
TTTCGAATCGCCAATAGCAC+AGG 0.520821 tig0025511:-68580 None:intergenic
AAATGCAGGCATAATGTTGA+TGG 0.521362 tig0025511:+68549 Msa1423100:intron
TCAATTGTTAGCAGTTATAC+GGG 0.521458 tig0025511:-69278 None:intergenic
TAATCCAAACCATCATTACA+TGG 0.521997 tig0025511:-69444 None:intergenic
TCTCTTGAGAAGCTGAATCT+CGG 0.522455 tig0025511:+67948 Msa1423100:CDS
AAAGGTGAAATAAACCGATT+CGG 0.523055 tig0025511:-67737 None:intergenic
TGATCATACCCATGTAATGA+TGG 0.530464 tig0025511:+69435 Msa1423100:three_prime_UTR
TGCTTAGAAAGATTTGAAGT+GGG 0.535088 tig0025511:+68235 Msa1423100:CDS
GGACAAGGATGACAAGGCAC+TGG 0.535550 tig0025511:+69018 Msa1423100:CDS
CACTAAAGGGAACACCGAAT+CGG 0.547540 tig0025511:+67723 None:intergenic
TCCTCAGGATCATATGACTT+AGG 0.553713 tig0025511:-68998 None:intergenic
AGAAAGCAATTCCAAAGTCT+CGG 0.562617 tig0025511:+68039 Msa1423100:CDS
GTGTGGGACACTATCAAGAA+AGG 0.563403 tig0025511:+68803 Msa1423100:CDS
TCCTAAGTCATATGATCCTG+AGG 0.564715 tig0025511:+68997 Msa1423100:CDS
GACATATGCAAGTACTTGAA+AGG 0.564755 tig0025511:+69148 Msa1423100:CDS
AGGCTTCTTATTGTTCTCCA+AGG 0.569260 tig0025511:-68756 None:intergenic
GTCATATGATCCTGAGGACA+AGG 0.573937 tig0025511:+69003 Msa1423100:CDS
TATGTGAAGGATAATGCTAT+TGG 0.576186 tig0025511:+69199 Msa1423100:CDS
GACAAGGATGACAAGGCACT+GGG 0.585950 tig0025511:+69019 Msa1423100:CDS
TTCAATCTATAAAATGTAGA+CGG 0.589902 tig0025511:-69664 None:intergenic
ATTATGTTACAGTTTACAAG+AGG 0.597781 tig0025511:+68185 Msa1423100:intron
AATAGCATTATCCTTCACAT+AGG 0.611395 tig0025511:-69197 None:intergenic
TCCTGAGGACAAGGATGACA+AGG 0.614403 tig0025511:+69012 Msa1423100:CDS
CCAAAGTCAAATTCGAACCA+TGG 0.623759 tig0025511:-69541 None:intergenic
AGATGTTCAGTCCTATGTGA+AGG 0.629750 tig0025511:+69186 Msa1423100:CDS
AATCCAAACCATCATTACAT+GGG 0.675720 tig0025511:-69443 None:intergenic
GTGACGATATTTGTAATCAG+CGG 0.676232 tig0025511:-68063 None:intergenic
GCTAACAATTGAGTAAAACA+TGG 0.686479 tig0025511:+69289 Msa1423100:exon
TGGGACACTATCAAGAAAGG+AGG 0.686741 tig0025511:+68806 Msa1423100:CDS
TGTTGCATAATGCAATGCAG+TGG 0.711796 tig0025511:+69320 Msa1423100:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTCTTAAATTATTAT+CGG - tig0025511:68343-68362 None:intergenic 10.0%
!! CAAATTTAAAAAGAAAATAA+GGG - tig0025511:68488-68507 None:intergenic 10.0%
!! TCAAATTTAAAAAGAAAATA+AGG - tig0025511:68489-68508 None:intergenic 10.0%
!!! TCATTTTCTTAAATTATTAT+CGG - tig0025511:68343-68362 None:intergenic 10.0%
!! CAAATTTAAAAAGAAAATAA+GGG - tig0025511:68488-68507 None:intergenic 10.0%
!! TCAAATTTAAAAAGAAAATA+AGG - tig0025511:68489-68508 None:intergenic 10.0%
!!! TTGTTTCATACATTTAATTT+AGG + tig0025511:68700-68719 Msa1423100:intron 15.0%
!!! TCTCTAGAAAAATAAAATAT+AGG - tig0025511:69060-69079 None:intergenic 15.0%
!!! ATTTTATTTTTCTAGAGATT+TGG + tig0025511:69062-69081 Msa1423100:intron 15.0%
!!! TCTAAAATTTTCTTTATTGT+TGG + tig0025511:69407-69426 Msa1423100:three_prime_UTR 15.0%
!! TTATGTATTATAAGATCATT+TGG + tig0025511:69599-69618 Msa1423100:three_prime_UTR 15.0%
!!! TTGTTTCATACATTTAATTT+AGG + tig0025511:68700-68719 Msa1423100:intron 15.0%
!!! TCTCTAGAAAAATAAAATAT+AGG - tig0025511:69060-69079 None:intergenic 15.0%
!!! ATTTTATTTTTCTAGAGATT+TGG + tig0025511:69062-69081 Msa1423100:intron 15.0%
!!! TCTAAAATTTTCTTTATTGT+TGG + tig0025511:69407-69426 Msa1423100:three_prime_UTR 15.0%
!! TTATGTATTATAAGATCATT+TGG + tig0025511:69599-69618 Msa1423100:three_prime_UTR 15.0%
!! ATAATAAGCAATTTCTTCTT+AGG - tig0025511:67975-67994 None:intergenic 20.0%
!! TATATGTGAGAAACTTATTA+CGG - tig0025511:68094-68113 None:intergenic 20.0%
!! TTCATAAATTCTTCACTAAA+TGG - tig0025511:68211-68230 None:intergenic 20.0%
!! ATGAATTCAGTTGTAATTAT+AGG + tig0025511:68359-68378 Msa1423100:intron 20.0%
!!! ACATATCAAGAAATGATTTT+AGG - tig0025511:68655-68674 None:intergenic 20.0%
!!! ATACATTTAATTTAGGATCA+AGG + tig0025511:68707-68726 Msa1423100:intron 20.0%
!! GGAAAAATAATTGATGTATT+GGG - tig0025511:68980-68999 None:intergenic 20.0%
!! AGGAAAAATAATTGATGTAT+TGG - tig0025511:68981-69000 None:intergenic 20.0%
!! ATAATTTGAATAGATCCAAA+TGG + tig0025511:69124-69143 Msa1423100:intron 20.0%
!!! TGGATTAAGTTTCTTTTTAA+AGG + tig0025511:69460-69479 Msa1423100:three_prime_UTR 20.0%
!! TTCAATCTATAAAATGTAGA+CGG - tig0025511:69667-69686 None:intergenic 20.0%
!! ATAATAAGCAATTTCTTCTT+AGG - tig0025511:67975-67994 None:intergenic 20.0%
!! TATATGTGAGAAACTTATTA+CGG - tig0025511:68094-68113 None:intergenic 20.0%
!! TTCATAAATTCTTCACTAAA+TGG - tig0025511:68211-68230 None:intergenic 20.0%
!! ATGAATTCAGTTGTAATTAT+AGG + tig0025511:68359-68378 Msa1423100:intron 20.0%
!!! ACATATCAAGAAATGATTTT+AGG - tig0025511:68655-68674 None:intergenic 20.0%
!!! ATACATTTAATTTAGGATCA+AGG + tig0025511:68707-68726 Msa1423100:intron 20.0%
!! GGAAAAATAATTGATGTATT+GGG - tig0025511:68980-68999 None:intergenic 20.0%
!! AGGAAAAATAATTGATGTAT+TGG - tig0025511:68981-69000 None:intergenic 20.0%
!! ATAATTTGAATAGATCCAAA+TGG + tig0025511:69124-69143 Msa1423100:intron 20.0%
!!! TGGATTAAGTTTCTTTTTAA+AGG + tig0025511:69460-69479 Msa1423100:three_prime_UTR 20.0%
!! TTCAATCTATAAAATGTAGA+CGG - tig0025511:69667-69686 None:intergenic 20.0%
! TTTACTTGCTTTATAACAAG+TGG - tig0025511:67764-67783 None:intergenic 25.0%
! TGATACACTTTGCAAAATTA+TGG - tig0025511:67811-67830 None:intergenic 25.0%
! AAAAAAGAGAGTTGTAGTTA+TGG + tig0025511:67843-67862 Msa1423100:CDS 25.0%
! TTGCTTATTATGAACCTTAA+CGG + tig0025511:67984-68003 Msa1423100:CDS 25.0%
! ATTATGTTACAGTTTACAAG+AGG + tig0025511:68185-68204 Msa1423100:intron 25.0%
! TCTTAAATTATTATCGGTGT+CGG - tig0025511:68337-68356 None:intergenic 25.0%
! AAAACAAGCTACTATTTGTT+TGG + tig0025511:68601-68620 Msa1423100:CDS 25.0%
! AAACAAGCTACTATTTGTTT+GGG + tig0025511:68602-68621 Msa1423100:CDS 25.0%
!! AAAGTATTTGAAGCAGAATA+TGG - tig0025511:68833-68852 None:intergenic 25.0%
! AAGAAAAACAAGTGAATTAC+CGG - tig0025511:68893-68912 None:intergenic 25.0%
! AGGTGATTCATTATCTTATT+TGG + tig0025511:69480-69499 Msa1423100:three_prime_UTR 25.0%
! GCAATTTATTGTAAAATCCA+TGG + tig0025511:69524-69543 Msa1423100:three_prime_UTR 25.0%
! TTTACTTGCTTTATAACAAG+TGG - tig0025511:67764-67783 None:intergenic 25.0%
! TGATACACTTTGCAAAATTA+TGG - tig0025511:67811-67830 None:intergenic 25.0%
! AAAAAAGAGAGTTGTAGTTA+TGG + tig0025511:67843-67862 Msa1423100:CDS 25.0%
! TTGCTTATTATGAACCTTAA+CGG + tig0025511:67984-68003 Msa1423100:CDS 25.0%
! ATTATGTTACAGTTTACAAG+AGG + tig0025511:68185-68204 Msa1423100:intron 25.0%
! TCTTAAATTATTATCGGTGT+CGG - tig0025511:68337-68356 None:intergenic 25.0%
! AAAACAAGCTACTATTTGTT+TGG + tig0025511:68601-68620 Msa1423100:CDS 25.0%
! AAACAAGCTACTATTTGTTT+GGG + tig0025511:68602-68621 Msa1423100:CDS 25.0%
!! AAAGTATTTGAAGCAGAATA+TGG - tig0025511:68833-68852 None:intergenic 25.0%
! AAGAAAAACAAGTGAATTAC+CGG - tig0025511:68893-68912 None:intergenic 25.0%
! AGGTGATTCATTATCTTATT+TGG + tig0025511:69480-69499 Msa1423100:three_prime_UTR 25.0%
! GCAATTTATTGTAAAATCCA+TGG + tig0025511:69524-69543 Msa1423100:three_prime_UTR 25.0%
AAAGGTGAAATAAACCGATT+CGG - tig0025511:67740-67759 None:intergenic 30.0%
TGCTTTATAACAAGTGGAAA+AGG - tig0025511:67758-67777 None:intergenic 30.0%
TTTGCAAAGTGTATCAAGAA+TGG + tig0025511:67815-67834 Msa1423100:exon 30.0%
! TGCTTAGAAAGATTTGAAGT+GGG + tig0025511:68235-68254 Msa1423100:CDS 30.0%
! CTGCATTTAGAGTAACAAAA+GGG - tig0025511:68537-68556 None:intergenic 30.0%
! TTCAGTTCTTTGAAAAAGAG+AGG - tig0025511:68779-68798 None:intergenic 30.0%
TCAAAGAACTGAAAAAAGTG+TGG + tig0025511:68786-68805 Msa1423100:CDS 30.0%
! CAAAGAACTGAAAAAAGTGT+GGG + tig0025511:68787-68806 Msa1423100:CDS 30.0%
ATACAAGTCTTTCCTTAATC+CGG + tig0025511:68871-68890 Msa1423100:CDS 30.0%
AATAGCATTATCCTTCACAT+AGG - tig0025511:69200-69219 None:intergenic 30.0%
TATGTGAAGGATAATGCTAT+TGG + tig0025511:69199-69218 Msa1423100:CDS 30.0%
TCAATTGTTAGCAGTTATAC+GGG - tig0025511:69281-69300 None:intergenic 30.0%
CTCAATTGTTAGCAGTTATA+CGG - tig0025511:69282-69301 None:intergenic 30.0%
GCTAACAATTGAGTAAAACA+TGG + tig0025511:69289-69308 Msa1423100:exon 30.0%
AATCCAAACCATCATTACAT+GGG - tig0025511:69446-69465 None:intergenic 30.0%
TAATCCAAACCATCATTACA+TGG - tig0025511:69447-69466 None:intergenic 30.0%
AAAGGTGAAATAAACCGATT+CGG - tig0025511:67740-67759 None:intergenic 30.0%
TGCTTTATAACAAGTGGAAA+AGG - tig0025511:67758-67777 None:intergenic 30.0%
TTTGCAAAGTGTATCAAGAA+TGG + tig0025511:67815-67834 Msa1423100:exon 30.0%
! TGCTTAGAAAGATTTGAAGT+GGG + tig0025511:68235-68254 Msa1423100:CDS 30.0%
! CTGCATTTAGAGTAACAAAA+GGG - tig0025511:68537-68556 None:intergenic 30.0%
! TTCAGTTCTTTGAAAAAGAG+AGG - tig0025511:68779-68798 None:intergenic 30.0%
TCAAAGAACTGAAAAAAGTG+TGG + tig0025511:68786-68805 Msa1423100:CDS 30.0%
! CAAAGAACTGAAAAAAGTGT+GGG + tig0025511:68787-68806 Msa1423100:CDS 30.0%
ATACAAGTCTTTCCTTAATC+CGG + tig0025511:68871-68890 Msa1423100:CDS 30.0%
AATAGCATTATCCTTCACAT+AGG - tig0025511:69200-69219 None:intergenic 30.0%
TATGTGAAGGATAATGCTAT+TGG + tig0025511:69199-69218 Msa1423100:CDS 30.0%
TCAATTGTTAGCAGTTATAC+GGG - tig0025511:69281-69300 None:intergenic 30.0%
CTCAATTGTTAGCAGTTATA+CGG - tig0025511:69282-69301 None:intergenic 30.0%
GCTAACAATTGAGTAAAACA+TGG + tig0025511:69289-69308 Msa1423100:exon 30.0%
AATCCAAACCATCATTACAT+GGG - tig0025511:69446-69465 None:intergenic 30.0%
TAATCCAAACCATCATTACA+TGG - tig0025511:69447-69466 None:intergenic 30.0%
! CTGAACTTTTGTGATCATCA+TGG - tig0025511:67908-67927 None:intergenic 35.0%
TATGGAGAAGAAATCCGTTA+AGG - tig0025511:68001-68020 None:intergenic 35.0%
AGAAAGCAATTCCAAAGTCT+CGG + tig0025511:68039-68058 Msa1423100:CDS 35.0%
GTGACGATATTTGTAATCAG+CGG - tig0025511:68066-68085 None:intergenic 35.0%
CTGCTTAGAAAGATTTGAAG+TGG + tig0025511:68234-68253 Msa1423100:CDS 35.0%
! TGTAATTATAGGTGTCGTGT+CGG + tig0025511:68370-68389 Msa1423100:intron 35.0%
! CCTGCATTTAGAGTAACAAA+AGG - tig0025511:68538-68557 None:intergenic 35.0%
! CCTTTTGTTACTCTAAATGC+AGG + tig0025511:68535-68554 Msa1423100:intron 35.0%
AAATGCAGGCATAATGTTGA+TGG + tig0025511:68549-68568 Msa1423100:intron 35.0%
AGCTACTATTTGTTTGGGTA+CGG + tig0025511:68607-68626 Msa1423100:intron 35.0%
CAAGGTAAATGTAGAGATTC+TGG + tig0025511:68725-68744 Msa1423100:CDS 35.0%
!! AGATTCTGGCTTTAAATCCT+TGG + tig0025511:68739-68758 Msa1423100:CDS 35.0%
GGATTAAGGAAAGACTTGTA+TGG - tig0025511:68872-68891 None:intergenic 35.0%
ACAAGTGAATTACCGGATTA+AGG - tig0025511:68886-68905 None:intergenic 35.0%
TACTTGCATATGTCACCATT+TGG - tig0025511:69142-69161 None:intergenic 35.0%
GACATATGCAAGTACTTGAA+AGG + tig0025511:69148-69167 Msa1423100:CDS 35.0%
TTACAACATCTCATCCTGAT+TGG + tig0025511:69233-69252 Msa1423100:CDS 35.0%
TACAACATCTCATCCTGATT+GGG + tig0025511:69234-69253 Msa1423100:CDS 35.0%
TGATCATACCCATGTAATGA+TGG + tig0025511:69435-69454 Msa1423100:three_prime_UTR 35.0%
ATACCCATGTAATGATGGTT+TGG + tig0025511:69440-69459 Msa1423100:three_prime_UTR 35.0%
! CATGGTTCGAATTTGACTTT+GGG + tig0025511:69542-69561 Msa1423100:three_prime_UTR 35.0%
! CTGAACTTTTGTGATCATCA+TGG - tig0025511:67908-67927 None:intergenic 35.0%
TATGGAGAAGAAATCCGTTA+AGG - tig0025511:68001-68020 None:intergenic 35.0%
AGAAAGCAATTCCAAAGTCT+CGG + tig0025511:68039-68058 Msa1423100:CDS 35.0%
GTGACGATATTTGTAATCAG+CGG - tig0025511:68066-68085 None:intergenic 35.0%
CTGCTTAGAAAGATTTGAAG+TGG + tig0025511:68234-68253 Msa1423100:CDS 35.0%
! TGTAATTATAGGTGTCGTGT+CGG + tig0025511:68370-68389 Msa1423100:intron 35.0%
! CCTGCATTTAGAGTAACAAA+AGG - tig0025511:68538-68557 None:intergenic 35.0%
! CCTTTTGTTACTCTAAATGC+AGG + tig0025511:68535-68554 Msa1423100:intron 35.0%
AAATGCAGGCATAATGTTGA+TGG + tig0025511:68549-68568 Msa1423100:intron 35.0%
AGCTACTATTTGTTTGGGTA+CGG + tig0025511:68607-68626 Msa1423100:intron 35.0%
CAAGGTAAATGTAGAGATTC+TGG + tig0025511:68725-68744 Msa1423100:CDS 35.0%
!! AGATTCTGGCTTTAAATCCT+TGG + tig0025511:68739-68758 Msa1423100:CDS 35.0%
GGATTAAGGAAAGACTTGTA+TGG - tig0025511:68872-68891 None:intergenic 35.0%
ACAAGTGAATTACCGGATTA+AGG - tig0025511:68886-68905 None:intergenic 35.0%
TACTTGCATATGTCACCATT+TGG - tig0025511:69142-69161 None:intergenic 35.0%
GACATATGCAAGTACTTGAA+AGG + tig0025511:69148-69167 Msa1423100:CDS 35.0%
TTACAACATCTCATCCTGAT+TGG + tig0025511:69233-69252 Msa1423100:CDS 35.0%
TACAACATCTCATCCTGATT+GGG + tig0025511:69234-69253 Msa1423100:CDS 35.0%
TGATCATACCCATGTAATGA+TGG + tig0025511:69435-69454 Msa1423100:three_prime_UTR 35.0%
ATACCCATGTAATGATGGTT+TGG + tig0025511:69440-69459 Msa1423100:three_prime_UTR 35.0%
! CATGGTTCGAATTTGACTTT+GGG + tig0025511:69542-69561 Msa1423100:three_prime_UTR 35.0%
AAGTTCAGCAGAACCAGAAT+TGG + tig0025511:67920-67939 Msa1423100:CDS 40.0%
AGTTCAGCAGAACCAGAATT+GGG + tig0025511:67921-67940 Msa1423100:CDS 40.0%
TCAAGAGAAAGACCCAATTC+TGG - tig0025511:67936-67955 None:intergenic 40.0%
TCTCTTGAGAAGCTGAATCT+CGG + tig0025511:67948-67967 Msa1423100:CDS 40.0%
! AAAGATTTGAAGTGGGTGTC+TGG + tig0025511:68242-68261 Msa1423100:CDS 40.0%
AACACTCTTCCTATGAAGCA+CGG + tig0025511:68289-68308 Msa1423100:intron 40.0%
AGGCTTCTTATTGTTCTCCA+AGG - tig0025511:68759-68778 None:intergenic 40.0%
ATGTATTGGGCTGCAATTTG+AGG - tig0025511:68967-68986 None:intergenic 40.0%
TCCTAAGTCATATGATCCTG+AGG + tig0025511:68997-69016 Msa1423100:CDS 40.0%
TCCTCAGGATCATATGACTT+AGG - tig0025511:69001-69020 None:intergenic 40.0%
AGATGTTCAGTCCTATGTGA+AGG + tig0025511:69186-69205 Msa1423100:CDS 40.0%
GGATGAGATGTTGTAATTGC+TGG - tig0025511:69229-69248 None:intergenic 40.0%
TGTTGCATAATGCAATGCAG+TGG + tig0025511:69320-69339 Msa1423100:three_prime_UTR 40.0%
CCAAAGTCAAATTCGAACCA+TGG - tig0025511:69544-69563 None:intergenic 40.0%
! CCATGGTTCGAATTTGACTT+TGG + tig0025511:69541-69560 Msa1423100:three_prime_UTR 40.0%
AAGTTCAGCAGAACCAGAAT+TGG + tig0025511:67920-67939 Msa1423100:CDS 40.0%
AGTTCAGCAGAACCAGAATT+GGG + tig0025511:67921-67940 Msa1423100:CDS 40.0%
TCAAGAGAAAGACCCAATTC+TGG - tig0025511:67936-67955 None:intergenic 40.0%
TCTCTTGAGAAGCTGAATCT+CGG + tig0025511:67948-67967 Msa1423100:CDS 40.0%
! AAAGATTTGAAGTGGGTGTC+TGG + tig0025511:68242-68261 Msa1423100:CDS 40.0%
AACACTCTTCCTATGAAGCA+CGG + tig0025511:68289-68308 Msa1423100:intron 40.0%
AGGCTTCTTATTGTTCTCCA+AGG - tig0025511:68759-68778 None:intergenic 40.0%
ATGTATTGGGCTGCAATTTG+AGG - tig0025511:68967-68986 None:intergenic 40.0%
TCCTAAGTCATATGATCCTG+AGG + tig0025511:68997-69016 Msa1423100:CDS 40.0%
TCCTCAGGATCATATGACTT+AGG - tig0025511:69001-69020 None:intergenic 40.0%
AGATGTTCAGTCCTATGTGA+AGG + tig0025511:69186-69205 Msa1423100:CDS 40.0%
GGATGAGATGTTGTAATTGC+TGG - tig0025511:69229-69248 None:intergenic 40.0%
TGTTGCATAATGCAATGCAG+TGG + tig0025511:69320-69339 Msa1423100:three_prime_UTR 40.0%
CCAAAGTCAAATTCGAACCA+TGG - tig0025511:69544-69563 None:intergenic 40.0%
! CCATGGTTCGAATTTGACTT+TGG + tig0025511:69541-69560 Msa1423100:three_prime_UTR 40.0%
TATGAAGCACGAACACTCCT+CGG - tig0025511:68438-68457 None:intergenic 45.0%
TTTCGAATCGCCAATAGCAC+AGG - tig0025511:68583-68602 None:intergenic 45.0%
GTGTGGGACACTATCAAGAA+AGG + tig0025511:68803-68822 Msa1423100:CDS 45.0%
TGGGACACTATCAAGAAAGG+AGG + tig0025511:68806-68825 Msa1423100:CDS 45.0%
GTCATATGATCCTGAGGACA+AGG + tig0025511:69003-69022 Msa1423100:CDS 45.0%
GGAACGAACACGAGAATAGA+AGG - tig0025511:69260-69279 None:intergenic 45.0%
TATGAAGCACGAACACTCCT+CGG - tig0025511:68438-68457 None:intergenic 45.0%
TTTCGAATCGCCAATAGCAC+AGG - tig0025511:68583-68602 None:intergenic 45.0%
GTGTGGGACACTATCAAGAA+AGG + tig0025511:68803-68822 Msa1423100:CDS 45.0%
TGGGACACTATCAAGAAAGG+AGG + tig0025511:68806-68825 Msa1423100:CDS 45.0%
GTCATATGATCCTGAGGACA+AGG + tig0025511:69003-69022 Msa1423100:CDS 45.0%
GGAACGAACACGAGAATAGA+AGG - tig0025511:69260-69279 None:intergenic 45.0%
TGTCAGTGACACGAGCTCTA+TGG - tig0025511:68019-68038 None:intergenic 50.0%
TAATCAGCGGTCCGAGACTT+TGG - tig0025511:68053-68072 None:intergenic 50.0%
TATGAAGCACGGACACGACA+CGG + tig0025511:68300-68319 Msa1423100:intron 50.0%
!! TATTATCGGTGTCGGCGTGT+GGG - tig0025511:68329-68348 None:intergenic 50.0%
!! TTATTATCGGTGTCGGCGTG+TGG - tig0025511:68330-68349 None:intergenic 50.0%
! TATAGGTGTCGTGTCGGTGT+CGG + tig0025511:68376-68395 Msa1423100:intron 50.0%
ATTAGGTATGTCCCGGTGTC+GGG - tig0025511:68415-68434 None:intergenic 50.0%
GATTAGGTATGTCCCGGTGT+CGG - tig0025511:68416-68435 None:intergenic 50.0%
! TCCTCGGATTAGGTATGTCC+CGG - tig0025511:68422-68441 None:intergenic 50.0%
GGTGCTTTAGCCTGTGCTAT+TGG + tig0025511:68570-68589 Msa1423100:CDS 50.0%
TCCTGAGGACAAGGATGACA+AGG + tig0025511:69012-69031 Msa1423100:CDS 50.0%
! GACAAGGATGACAAGGCACT+GGG + tig0025511:69019-69038 Msa1423100:CDS 50.0%
CGAGAATAGAAGGCCCAATC+AGG - tig0025511:69250-69269 None:intergenic 50.0%
TGTCAGTGACACGAGCTCTA+TGG - tig0025511:68019-68038 None:intergenic 50.0%
TAATCAGCGGTCCGAGACTT+TGG - tig0025511:68053-68072 None:intergenic 50.0%
TATGAAGCACGGACACGACA+CGG + tig0025511:68300-68319 Msa1423100:intron 50.0%
!! TATTATCGGTGTCGGCGTGT+GGG - tig0025511:68329-68348 None:intergenic 50.0%
!! TTATTATCGGTGTCGGCGTG+TGG - tig0025511:68330-68349 None:intergenic 50.0%
! TATAGGTGTCGTGTCGGTGT+CGG + tig0025511:68376-68395 Msa1423100:intron 50.0%
ATTAGGTATGTCCCGGTGTC+GGG - tig0025511:68415-68434 None:intergenic 50.0%
GATTAGGTATGTCCCGGTGT+CGG - tig0025511:68416-68435 None:intergenic 50.0%
! TCCTCGGATTAGGTATGTCC+CGG - tig0025511:68422-68441 None:intergenic 50.0%
GGTGCTTTAGCCTGTGCTAT+TGG + tig0025511:68570-68589 Msa1423100:CDS 50.0%
TCCTGAGGACAAGGATGACA+AGG + tig0025511:69012-69031 Msa1423100:CDS 50.0%
! GACAAGGATGACAAGGCACT+GGG + tig0025511:69019-69038 Msa1423100:CDS 50.0%
CGAGAATAGAAGGCCCAATC+AGG - tig0025511:69250-69269 None:intergenic 50.0%
! GGGTGTCTGGTCTTCTGCAA+TGG + tig0025511:68255-68274 Msa1423100:CDS 55.0%
GTGTCGTGTCCGTGCTTCAT+AGG - tig0025511:68301-68320 None:intergenic 55.0%
ACCGGGACATACCTAATCCG+AGG + tig0025511:68418-68437 Msa1423100:intron 55.0%
GCACGAACACTCCTCGGATT+AGG - tig0025511:68432-68451 None:intergenic 55.0%
GCCTTGTCATCCTTGTCCTC+AGG - tig0025511:69016-69035 None:intergenic 55.0%
GGACAAGGATGACAAGGCAC+TGG + tig0025511:69018-69037 Msa1423100:CDS 55.0%
! GGGTGTCTGGTCTTCTGCAA+TGG + tig0025511:68255-68274 Msa1423100:CDS 55.0%
GTGTCGTGTCCGTGCTTCAT+AGG - tig0025511:68301-68320 None:intergenic 55.0%
ACCGGGACATACCTAATCCG+AGG + tig0025511:68418-68437 Msa1423100:intron 55.0%
GCACGAACACTCCTCGGATT+AGG - tig0025511:68432-68451 None:intergenic 55.0%
GCCTTGTCATCCTTGTCCTC+AGG - tig0025511:69016-69035 None:intergenic 55.0%
GGACAAGGATGACAAGGCAC+TGG + tig0025511:69018-69037 Msa1423100:CDS 55.0%
!! CACCGACTCGTACCCGACAC+CGG + tig0025511:68400-68419 Msa1423100:intron 65.0%
!! ACCGACTCGTACCCGACACC+GGG + tig0025511:68401-68420 Msa1423100:intron 65.0%
! TCCCGGTGTCGGGTACGAGT+CGG - tig0025511:68405-68424 None:intergenic 65.0%
!! CACCGACTCGTACCCGACAC+CGG + tig0025511:68400-68419 Msa1423100:intron 65.0%
!! ACCGACTCGTACCCGACACC+GGG + tig0025511:68401-68420 Msa1423100:intron 65.0%
! TCCCGGTGTCGGGTACGAGT+CGG - tig0025511:68405-68424 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0025511 gene 67737 69697 67737 ID=Msa1423100;Name=Msa1423100
tig0025511 mRNA 67737 69697 67737 ID=Msa1423100-mRNA-1;Parent=Msa1423100;Name=Msa1423100-mRNA-1;_AED=0.25;_eAED=0.25;_QI=97|1|1|1|1|1|6|397|268
tig0025511 exon 67737 68092 67737 ID=Msa1423100-mRNA-1:exon:3006;Parent=Msa1423100-mRNA-1
tig0025511 exon 68197 68278 68197 ID=Msa1423100-mRNA-1:exon:3007;Parent=Msa1423100-mRNA-1
tig0025511 exon 68557 68623 68557 ID=Msa1423100-mRNA-1:exon:3008;Parent=Msa1423100-mRNA-1
tig0025511 exon 68722 68892 68722 ID=Msa1423100-mRNA-1:exon:3009;Parent=Msa1423100-mRNA-1
tig0025511 exon 68977 69040 68977 ID=Msa1423100-mRNA-1:exon:3010;Parent=Msa1423100-mRNA-1
tig0025511 exon 69137 69697 69137 ID=Msa1423100-mRNA-1:exon:3011;Parent=Msa1423100-mRNA-1
tig0025511 five_prime_UTR 67737 67833 67737 ID=Msa1423100-mRNA-1:five_prime_utr;Parent=Msa1423100-mRNA-1
tig0025511 CDS 67834 68092 67834 ID=Msa1423100-mRNA-1:cds;Parent=Msa1423100-mRNA-1
tig0025511 CDS 68197 68278 68197 ID=Msa1423100-mRNA-1:cds;Parent=Msa1423100-mRNA-1
tig0025511 CDS 68557 68623 68557 ID=Msa1423100-mRNA-1:cds;Parent=Msa1423100-mRNA-1
tig0025511 CDS 68722 68892 68722 ID=Msa1423100-mRNA-1:cds;Parent=Msa1423100-mRNA-1
tig0025511 CDS 68977 69040 68977 ID=Msa1423100-mRNA-1:cds;Parent=Msa1423100-mRNA-1
tig0025511 CDS 69137 69300 69137 ID=Msa1423100-mRNA-1:cds;Parent=Msa1423100-mRNA-1
tig0025511 three_prime_UTR 69301 69697 69301 ID=Msa1423100-mRNA-1:three_prime_utr;Parent=Msa1423100-mRNA-1
Gene Sequence

>Msa1423100

ATGGAAGCAAAAAAAGAGAGTTGTAGTTATGGAAATATCAAAATCAAAGACTCTGAAGCTGATCATGATGACCATGATGATCACAAAAGTTCAGCAGAACCAGAATTGGGTCTTTCTCTTGAGAAGCTGAATCTCGGACCTAAGAAGAAATTGCTTATTATGAACCTTAACGGATTTCTTCTCCATAGAGCTCGTGTCACTGACAAGAAAGCAATTCCAAAGTCTCGGACCGCTGATTACAAATATCGTCACTTTCTCCTTTACAAGAGGCCATTTAGTGAAGAATTTATGAAATTCTGCTTAGAAAGATTTGAAGTGGGTGTCTGGTCTTCTGCAATGGAGCATAATGTTGATGGTGCTTTAGCCTGTGCTATTGGCGATTCGAAAAACAAGCTACTATTTGTTTGGGATCAAGGTAAATGTAGAGATTCTGGCTTTAAATCCTTGGAGAACAATAAGAAGCCTCTCTTTTTCAAAGAACTGAAAAAAGTGTGGGACACTATCAAGAAAGGAGGACCATATTCTGCTTCAAATACTTTGCTGATCGATGACAAACCATACAAGTCTTTCCTTAATCCGCCCAATACATCAATTATTTTTCCTAAGTCATATGATCCTGAGGACAAGGATGACAAGGCACTGGATCCAAATGGTGACATATGCAAGTACTTGAAAGGAGTAGCTGAAGCTGAAGATGTTCAGTCCTATGTGAAGGATAATGCTATTGGTGAACCAGCAATTACAACATCTCATCCTGATTGGGCCTTCTATTCTCGTGTTCGTTCCCGTATAACTGCTAACAATTGA
Protein sequence

>Msa1423100

MEAKKESCSYGNIKIKDSEADHDDHDDHKSSAEPELGLSLEKLNLGPKKKLLIMNLNGFLLHRARVTDKKAIPKSRTADYKYRHFLLYKRPFSEEFMKFCLERFEVGVWSSAMEHNVDGALACAIGDSKNKLLFVWDQGKCRDSGFKSLENNKKPLFFKELKKVWDTIKKGGPYSASNTLLIDDKPYKSFLNPPNTSIIFPKSYDPEDKDDKALDPNGDICKYLKGVAEAEDVQSYVKDNAIGEPAITTSHPDWAFYSRVRSRITANN*