AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0974640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0974640 MtrunA17_Chr6g0453321 94.726 493 26 0 2 494 3 495 0.0 951
Msa0974640 MtrunA17_Chr7g0217581 55.992 509 185 9 1 494 99 583 1.69e-176 508
Msa0974640 MtrunA17_Chr4g0042481 44.048 504 226 17 2 492 3 463 5.74e-117 352
Msa0974640 MtrunA17_Chr3g0092111 42.197 519 220 19 1 494 2 465 1.75e-97 302
Msa0974640 MtrunA17_Chr7g0254161 36.789 492 214 20 4 474 8 423 1.04e-66 221
Msa0974640 MtrunA17_Chr8g0355261 37.696 382 180 14 122 493 105 438 1.82e-65 218
Msa0974640 MtrunA17_Chr5g0417601 36.580 421 145 12 73 492 80 379 5.01e-64 212
Msa0974640 MtrunA17_Chr5g0417551 35.990 414 136 16 85 492 79 369 1.16e-56 192
Msa0974640 MtrunA17_Chr5g0417561 36.124 418 130 17 85 492 71 361 3.68e-56 191
Msa0974640 MtrunA17_Chr6g0460731 39.683 252 116 8 92 327 51 282 1.32e-44 160
Msa0974640 MtrunA17_Chr6g0460721 41.393 244 110 9 95 329 47 266 2.28e-42 153
Msa0974640 MtrunA17_Chr5g0417571 43.137 204 102 5 8 199 7 208 5.83e-41 149
Msa0974640 MtrunA17_Chr2g0283811 81.538 65 11 1 125 188 36 100 8.48e-34 125
Msa0974640 MtrunA17_Chr3g0127201 67.742 62 20 0 130 191 22 83 5.32e-26 107
Msa0974640 MtrunA17_Chr8g0374251 75.926 54 13 0 135 188 68 121 2.97e-25 106
Msa0974640 MtrunA17_Chr8g0374111 44.915 118 53 3 135 243 29 143 3.36e-25 103
Msa0974640 MtrunA17_Chr2g0328021 50.549 91 44 1 99 188 25 115 3.79e-25 105
Msa0974640 MtrunA17_Chr8g0346551 50.538 93 36 1 130 212 19 111 5.01e-25 104
Msa0974640 MtrunA17_Chr4g0010151 68.966 58 18 0 131 188 78 135 5.72e-25 106
Msa0974640 MtrunA17_Chr4g0031411 51.087 92 43 1 128 217 68 159 1.66e-24 104
Msa0974640 MtrunA17_Chr2g0326001 69.492 59 18 0 130 188 35 93 1.70e-24 103
Msa0974640 MtrunA17_Chr2g0308011 77.551 49 11 0 135 183 37 85 2.29e-24 100
Msa0974640 MtrunA17_Chr8g0341671 75.000 52 13 0 135 186 20 71 2.61e-24 100
Msa0974640 MtrunA17_Chr4g0061761 69.811 53 16 0 133 185 38 90 7.41e-24 102
Msa0974640 MtrunA17_Chr1g0188851 62.712 59 22 0 129 187 37 95 1.81e-23 100
Msa0974640 MtrunA17_Chr7g0223311 76.471 51 12 0 137 187 79 129 2.41e-23 101
Msa0974640 MtrunA17_Chr4g0047001 75.000 52 13 0 137 188 57 108 3.55e-23 101
Msa0974640 MtrunA17_Chr3g0126201 58.108 74 20 1 137 210 53 115 3.99e-23 100
Msa0974640 MtrunA17_Chr8g0367161 47.368 95 45 2 123 217 20 109 4.79e-23 100
Msa0974640 MtrunA17_Chr5g0411951 67.273 55 18 0 131 185 39 93 6.02e-23 99.8
Msa0974640 MtrunA17_Chr7g0237921 71.154 52 15 0 137 188 78 129 1.30e-22 99.0
Msa0974640 MtrunA17_Chr2g0282511 55.844 77 31 1 107 183 20 93 1.53e-22 98.2
Msa0974640 MtrunA17_Chr3g0117601 45.455 110 53 1 97 206 30 132 1.58e-22 98.6
Msa0974640 MtrunA17_Chr1g0177251 54.321 81 34 2 130 207 6 86 1.61e-22 97.4
Msa0974640 MtrunA17_Chr4g0030051 65.625 64 21 1 125 187 30 93 2.89e-22 96.7
Msa0974640 MtrunA17_Chr2g0282051 73.077 52 13 1 137 188 48 98 9.19e-22 95.1
Msa0974640 MtrunA17_Chr4g0047761 66.667 54 18 0 135 188 72 125 9.21e-22 95.9
Msa0974640 MtrunA17_Chr2g0282441 70.000 50 15 0 137 186 27 76 1.97e-21 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0974640 AT5G39660.3 42.075 530 192 24 5 494 3 457 2.88e-104 319
Msa0974640 AT5G39660.1 42.075 530 192 24 5 494 3 457 2.88e-104 319
Msa0974640 AT5G39660.2 42.075 530 192 24 5 494 3 457 2.88e-104 319
Msa0974640 AT3G47500.1 39.173 508 229 19 1 492 3 446 5.44e-82 261
Msa0974640 AT5G62430.1 37.405 393 119 17 105 492 26 296 1.89e-54 185
Msa0974640 AT1G69570.1 34.677 372 137 14 128 493 125 396 5.50e-46 165
Msa0974640 AT1G26790.1 57.463 134 48 3 80 209 59 187 9.01e-38 142
Msa0974640 AT1G29160.1 86.667 60 8 0 129 188 56 115 3.82e-35 129
Msa0974640 AT2G34140.1 83.333 60 10 0 129 188 52 111 1.94e-33 125
Msa0974640 AT5G60850.1 61.429 70 27 0 123 192 39 108 4.72e-26 108
Msa0974640 AT1G51700.1 72.881 59 15 1 135 192 33 91 1.94e-25 103
Msa0974640 AT3G55370.1 60.870 69 27 0 131 199 72 140 3.14e-25 106
Msa0974640 AT3G55370.2 60.870 69 27 0 131 199 72 140 3.39e-25 107
Msa0974640 AT5G60200.1 55.172 87 37 2 131 215 51 137 4.85e-25 104
Msa0974640 AT3G55370.3 60.870 69 27 0 131 199 117 185 5.70e-25 106
Msa0974640 AT3G21270.1 77.358 53 12 0 135 187 29 81 1.35e-24 101
Msa0974640 AT1G07640.1 61.111 72 25 1 116 187 5 73 1.39e-24 103
Msa0974640 AT3G45610.1 56.944 72 30 1 131 201 36 107 1.56e-24 102
Msa0974640 AT2G28810.2 65.574 61 21 0 126 186 68 128 1.58e-24 104
Msa0974640 AT2G28810.1 65.574 61 21 0 126 186 85 145 2.21e-24 104
Msa0974640 AT3G61850.2 63.492 63 23 0 130 192 57 119 2.32e-24 103
Msa0974640 AT3G61850.3 63.492 63 23 0 130 192 57 119 2.32e-24 103
Msa0974640 AT3G61850.1 63.492 63 23 0 130 192 69 131 2.34e-24 103
Msa0974640 AT3G50410.1 73.585 53 14 0 135 187 30 82 3.01e-24 102
Msa0974640 AT3G61850.5 63.492 63 23 0 130 192 57 119 3.32e-24 103
Msa0974640 AT1G07640.2 75.472 53 13 0 135 187 77 129 3.36e-24 103
Msa0974640 AT3G61850.4 63.492 63 23 0 130 192 69 131 3.71e-24 103
Msa0974640 AT5G65590.1 73.214 56 15 0 135 190 41 96 4.10e-24 103
Msa0974640 AT1G07640.3 75.472 53 13 0 135 187 85 137 4.23e-24 103
Msa0974640 AT2G37590.1 67.241 58 19 0 131 188 85 142 4.29e-24 103
Msa0974640 AT4G38000.1 52.874 87 39 2 134 219 40 125 4.67e-24 101
Msa0974640 AT4G24060.1 68.421 57 18 0 132 188 50 106 6.81e-24 103
Msa0974640 AT1G64620.1 71.154 52 15 0 132 183 46 97 3.91e-23 100
Msa0974640 AT1G28310.1 72.222 54 15 0 135 188 27 80 4.28e-23 100
Msa0974640 AT5G02460.1 67.241 58 19 0 131 188 91 148 5.69e-23 101
Msa0974640 AT2G28510.1 69.643 56 17 0 137 192 50 105 6.47e-23 99.0
Msa0974640 AT1G28310.2 72.222 54 15 0 135 188 51 104 7.31e-23 99.8
Msa0974640 AT5G66940.1 67.925 53 17 0 135 187 32 84 2.01e-22 96.3
Msa0974640 AT4G00940.1 66.102 59 20 0 130 188 63 121 2.82e-22 97.4
Msa0974640 AT4G00940.3 66.102 59 20 0 130 188 63 121 2.82e-22 97.4
Msa0974640 AT4G00940.2 66.102 59 20 0 130 188 63 121 2.82e-22 97.4
Msa0974640 AT1G47655.1 73.469 49 13 0 135 183 29 77 3.48e-22 95.1
Msa0974640 AT2G46590.1 67.925 53 17 0 130 182 63 115 6.84e-22 97.4
Msa0974640 AT2G46590.2 67.925 53 17 0 130 182 75 127 7.08e-22 97.4
Msa0974640 AT3G52440.1 69.231 52 16 0 137 188 27 78 7.29e-22 95.1
Msa0974640 AT3G52440.2 69.231 52 16 0 137 188 46 97 1.03e-21 95.1
Msa0974640 AT1G21340.1 66.667 54 18 0 137 190 40 93 1.23e-21 94.7
Msa0974640 AT4G21050.1 57.353 68 29 0 121 188 10 77 4.57e-21 92.0
Msa0974640 AT5G62940.1 69.388 49 15 0 137 185 75 123 7.59e-20 91.7
Msa0974640 AT4G21040.1 49.412 85 36 2 137 221 27 104 8.70e-19 85.9
Msa0974640 AT4G21080.1 53.333 75 31 2 137 208 27 100 5.51e-18 84.0
Msa0974640 AT4G21030.1 56.818 44 19 0 137 180 23 66 7.02e-11 62.0

Find 158 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTCTGCATTCGCTTTA+AGG 0.100087 6_4:+7860275 Msa0974640:three_prime_UTR
TCTATCCTCCACCGGCTTAT+TGG 0.171714 6_4:+7859567 Msa0974640:CDS
CTATCCTCCACCGGCTTATT+GGG 0.204943 6_4:+7859568 Msa0974640:CDS
ATTGAAAACTTCAAAGATTA+TGG 0.206877 6_4:+7858845 Msa0974640:CDS
GGTGGGAAGCTTTGACAATT+TGG 0.253300 6_4:-7859431 None:intergenic
CTGTTTCGGGTACAGTTATT+TGG 0.254197 6_4:-7859103 None:intergenic
CATGGTAGCTTTCATGTTAC+AGG 0.256387 6_4:+7860657 Msa0974640:three_prime_UTR
GTTATTGTAGTAGCAGAATT+TGG 0.264718 6_4:-7858952 None:intergenic
GCAAGAAATGCCAGAGATAC+TGG 0.298374 6_4:+7859003 Msa0974640:CDS
CAAACAAACAAACGATTGTT+TGG 0.302076 6_4:-7860742 None:intergenic
GAGTTAACGGCTGCACTATT+TGG 0.304692 6_4:-7859644 None:intergenic
AGAATTCTGAACAGCTGTTT+CGG 0.322405 6_4:-7859117 None:intergenic
GCCGCAACCGCCGCCACCTA+TGG 0.323027 6_4:-7860333 None:intergenic
GAATTCTGAACAGCTGTTTC+GGG 0.323421 6_4:-7859116 None:intergenic
GCTGGATTGGCCTGCATGGC+AGG 0.325373 6_4:-7859941 None:intergenic
TTACCTAAGGTTGGCGAGTT+AGG 0.325633 6_4:-7859671 None:intergenic
CCGCAACCGCCGCCACCTAT+GGG 0.331027 6_4:-7860332 None:intergenic
CCCATAGGTGGCGGCGGTTG+CGG 0.332704 6_4:+7860332 Msa0974640:three_prime_UTR
GGTGTCCATGCTATTACAAC+GGG 0.366283 6_4:-7858931 None:intergenic
TTCATAGCTGCTGTGTCAAT+TGG 0.384604 6_4:-7860052 None:intergenic
TGCTGCTGGATTGGCCTGCA+TGG 0.384791 6_4:-7859945 None:intergenic
GCATTGAACCTTGCTGATAA+AGG 0.387819 6_4:+7859242 Msa0974640:CDS
GGAGCACCAGCTTCATCTTC+TGG 0.388137 6_4:+7857244 Msa0974640:CDS
CCTAGACATTGCTGCTGGAT+TGG 0.394113 6_4:-7859954 None:intergenic
AGCTCAATATTGACAACTCT+CGG 0.400154 6_4:+7859824 Msa0974640:CDS
ACATGAATCCGTAGACCTTC+CGG 0.402858 6_4:-7859299 None:intergenic
TGATGTGTCCGAAGATTTGA+TGG 0.404612 6_4:-7859357 None:intergenic
ATGGCACAGTCTTTACATTC+AGG 0.405324 6_4:+7859186 Msa0974640:CDS
CAACCTTAGGTAAGCATTCA+AGG 0.416860 6_4:+7859681 Msa0974640:CDS
CCCCGGCAGTCCAGTATCTC+TGG 0.422379 6_4:-7859013 None:intergenic
GGCAAAAGCCAAGGGGCACC+AGG 0.424018 6_4:-7859470 None:intergenic
TTCCCTTGAATGCTTACCTA+AGG 0.425560 6_4:-7859684 None:intergenic
ATATACATCGACGATCTTGC+AGG 0.429015 6_4:-7860457 None:intergenic
TTCTTTCTCTTCTTCATCCA+TGG 0.431823 6_4:-7857308 None:intergenic
AAGAAAACATTCTAAAAGAA+AGG 0.432175 6_4:-7860376 None:intergenic
CGAGTTAGGACCGGAGTTAA+CGG 0.433388 6_4:-7859657 None:intergenic
TTCATTGCTCTTTGAACCAA+AGG 0.434809 6_4:-7859897 None:intergenic
AGACACAAACTCAAGATTAG+TGG 0.448603 6_4:+7858782 Msa0974640:CDS
ATAAAGAAGAAAAGTCCCTT+TGG 0.450455 6_4:+7859756 Msa0974640:CDS
TGGGAAGACAATTCCGGTAC+CGG 0.459321 6_4:+7857192 Msa0974640:CDS
TCCGGTAGAGTCACCGGATC+CGG 0.459421 6_4:-7857224 None:intergenic
GCCAGAGATACTGGACTGCC+GGG 0.460238 6_4:+7859012 Msa0974640:CDS
CCGGAGATCTCTGCCGGATC+CGG 0.461337 6_4:+7857211 Msa0974640:CDS
TGCCGTGGAATCCATCTCAA+TGG 0.462888 6_4:+7859489 Msa0974640:CDS
CTCAAACCAACCGACTCAAC+AGG 0.464405 6_4:+7859716 Msa0974640:CDS
TAAAGAAGAAAAGTCCCTTT+GGG 0.467980 6_4:+7859757 Msa0974640:CDS
AAACAAACGATTGTTTGGTT+CGG 0.469157 6_4:-7860737 None:intergenic
CCATGGATTGTTCCACGCAC+CGG 0.474496 6_4:-7859603 None:intergenic
GGTCCTAACTCGCCAACCTT+AGG 0.475475 6_4:+7859668 Msa0974640:CDS
GCAAAACTCACCTGCCATGC+AGG 0.477299 6_4:+7859931 Msa0974640:CDS
TCAGAACCTGTTGAGTCGGT+TGG 0.477638 6_4:-7859722 None:intergenic
TGCCAGAGATACTGGACTGC+CGG 0.478106 6_4:+7859011 Msa0974640:CDS
TAATCATGTAAACGTGACTT+TGG 0.478426 6_4:+7860623 Msa0974640:three_prime_UTR
AATGGAAGATATTGAGAAGA+TGG 0.482463 6_4:+7860252 Msa0974640:three_prime_UTR
TGATCATTCCATCAAATCTT+CGG 0.483222 6_4:+7859349 Msa0974640:CDS
CTTGAATGCTTACCTAAGGT+TGG 0.484913 6_4:-7859680 None:intergenic
CACATTTCTCATGGCTCCCC+CGG 0.486523 6_4:-7859030 None:intergenic
GAAGGATGATGGACTCCGTT+TGG 0.487346 6_4:-7859152 None:intergenic
AACTTGTTCAAGAGAACTGC+TGG 0.493603 6_4:-7859405 None:intergenic
TTCCTACGACCGGCACCTAC+AGG 0.494832 6_4:-7859053 None:intergenic
TGGATAACTATGCATGTGTT+TGG 0.495117 6_4:+7860581 Msa0974640:three_prime_UTR
TACAGTTCAGTGAAGGATGA+TGG 0.503313 6_4:-7859163 None:intergenic
TTCGTCAGAACCTGTTGAGT+CGG 0.506659 6_4:-7859726 None:intergenic
GCCGGATCCGGTGACTCTAC+CGG 0.509037 6_4:+7857223 Msa0974640:CDS
TCTAAACCATGGTTCTTCCA+TGG 0.512969 6_4:+7857291 Msa0974640:CDS
TCTCAATCCGACTCGCCAAA+CGG 0.513729 6_4:+7859137 Msa0974640:CDS
AGAAAACATTCTAAAAGAAA+GGG 0.514268 6_4:-7860375 None:intergenic
TCTGGTGATGCTGTTGATGA+TGG 0.515932 6_4:+7857262 Msa0974640:CDS
CATGGATTGTTCCACGCACC+GGG 0.516259 6_4:-7859602 None:intergenic
TTATAAGCTACATAATTATG+AGG 0.516746 6_4:-7859996 None:intergenic
ATGGACTCCGTTTGGCGAGT+CGG 0.520233 6_4:-7859144 None:intergenic
TCATTAGACCGGAAGGTCTA+CGG 0.522416 6_4:+7859291 Msa0974640:CDS
CCAATCCAGCAGCAATGTCT+AGG 0.524624 6_4:+7859954 Msa0974640:CDS
TCTCACCCAATCATCAGAAA+AGG 0.525594 6_4:+7858749 Msa0974640:CDS
CATCCTTCACTGAACTGTAA+TGG 0.526917 6_4:+7859167 Msa0974640:CDS
AATAAGGCGGACGCGATTCG+TGG 0.530810 6_4:+7859857 Msa0974640:CDS
CCGGCAGAGATCTCCGGTAC+CGG 0.531399 6_4:-7857205 None:intergenic
ATTGACCTAGACATTGCTGC+TGG 0.532105 6_4:-7859959 None:intergenic
AGAAGATGAAGCTGGTGCTC+CGG 0.532773 6_4:-7857242 None:intergenic
GCTGCACTATTTGGTGAAGA+AGG 0.534001 6_4:-7859635 None:intergenic
ACTTGAGAGCTCCATTGAGA+TGG 0.535364 6_4:-7859500 None:intergenic
GTGGCATGGCAAATCCTTGT+GGG 0.536245 6_4:-7859544 None:intergenic
GCTCAATATTGACAACTCTC+GGG 0.536746 6_4:+7859825 Msa0974640:CDS
TGGTGCTCCGGTAGAGTCAC+CGG 0.536837 6_4:-7857230 None:intergenic
TTAGGACGCCGTCTTGAACA+TGG 0.539519 6_4:+7860711 Msa0974640:three_prime_UTR
TGCGTGGAACAATCCATGGC+TGG 0.539604 6_4:+7859607 Msa0974640:CDS
ATAAGGCGGACGCGATTCGT+GGG 0.543719 6_4:+7859858 Msa0974640:CDS
CAAGGGGCACCAGGGTAGTA+CGG 0.543849 6_4:-7859461 None:intergenic
CTCCATTGAGATGGATTCCA+CGG 0.551011 6_4:-7859491 None:intergenic
AGGTGGCGGCGGTTGCGGCA+AGG 0.551235 6_4:+7860337 Msa0974640:three_prime_UTR
TTTCACCCCATAGGTGGCGG+CGG 0.553348 6_4:+7860326 Msa0974640:three_prime_UTR
TGAATCGACCAAATGTAAGA+AGG 0.555366 6_4:+7858713 Msa0974640:CDS
TATTAACTCCTTTATCAGCA+AGG 0.557069 6_4:-7859250 None:intergenic
TATCCTCCACCGGCTTATTG+GGG 0.557577 6_4:+7859569 Msa0974640:CDS
CGCAACCGCCGCCACCTATG+GGG 0.558906 6_4:-7860331 None:intergenic
ATTCTTGTTCTTCCTACGAC+CGG 0.559859 6_4:-7859063 None:intergenic
CTTCATCCATGGAAGAACCA+TGG 0.559893 6_4:-7857297 None:intergenic
GCAGAGTAGTTTCACCCCAT+AGG 0.560861 6_4:+7860317 Msa0974640:three_prime_UTR
ATGCTAAAACCCCAATAAGC+CGG 0.561380 6_4:-7859578 None:intergenic
TGGTGTCCATGCTATTACAA+CGG 0.562401 6_4:-7858932 None:intergenic
ATCATGGAAGCAAAAGACCC+TGG 0.564274 6_4:+7857157 Msa0974640:exon
GGATTCCACGGCAAAAGCCA+AGG 0.568149 6_4:-7859479 None:intergenic
TGCCTGTAGGTGCCGGTCGT+AGG 0.569339 6_4:+7859051 Msa0974640:CDS
TTTGCTCGTCCTCAGTGGAG+GGG 0.569766 6_4:-7858818 None:intergenic
CGGATTCATGTACCCTATGT+TGG 0.570976 6_4:+7859311 Msa0974640:CDS
TAGTTTCACCCCATAGGTGG+CGG 0.573607 6_4:+7860323 Msa0974640:three_prime_UTR
GTGTCCCCGTTGTAATAGCA+TGG 0.578322 6_4:+7858926 Msa0974640:CDS
AAACCCCAATAAGCCGGTGG+AGG 0.580141 6_4:-7859572 None:intergenic
ACCTACAGGCACATTTCTCA+TGG 0.581950 6_4:-7859039 None:intergenic
GGGTAGTACGGAACTTGCGG+TGG 0.584630 6_4:-7859449 None:intergenic
AACGGAATCATTAGACCGGA+AGG 0.586342 6_4:+7859284 Msa0974640:CDS
ACTTTGGAGAAGCAGCTACA+TGG 0.588467 6_4:+7860639 Msa0974640:three_prime_UTR
TAAGGTTGGCGAGTTAGGAC+CGG 0.591433 6_4:-7859666 None:intergenic
CCGCAAGTTCCGTACTACCC+TGG 0.592010 6_4:+7859452 Msa0974640:CDS
TCATTTCGGTGCAATAAACA+TGG 0.593061 6_4:+7860542 Msa0974640:three_prime_UTR
CCGGTGCGTGGAACAATCCA+TGG 0.594068 6_4:+7859603 Msa0974640:CDS
AGAAATGTGCCTGTAGGTGC+CGG 0.595484 6_4:+7859044 Msa0974640:CDS
ACGTTGAGGATTGATGACTT+AGG 0.598062 6_4:+7859788 Msa0974640:CDS
GCATCACCAGAAGATGAAGC+TGG 0.603079 6_4:-7857250 None:intergenic
TCATGGAAGCAAAAGACCCT+GGG 0.603420 6_4:+7857158 Msa0974640:exon
CCAGGGTAGTACGGAACTTG+CGG 0.603954 6_4:-7859452 None:intergenic
AGAAGAGAAAGAAATTGATG+AGG 0.604766 6_4:+7857318 Msa0974640:CDS
TACAAACTGTAAGAATACAT+TGG 0.604770 6_4:+7860190 Msa0974640:three_prime_UTR
GATTCCACGGCAAAAGCCAA+GGG 0.605584 6_4:-7859478 None:intergenic
AATAGTGCAGCCGTTAACTC+CGG 0.609641 6_4:+7859647 Msa0974640:CDS
AACCTTAGGTAAGCATTCAA+GGG 0.609767 6_4:+7859682 Msa0974640:CDS
TGTTTGGTAGTGCATTACAT+TGG 0.610643 6_4:+7860597 Msa0974640:three_prime_UTR
GAAGAAGGTTGTGCCAGCCA+TGG 0.610893 6_4:-7859620 None:intergenic
ATTTGAGAAAATGAAAGTGT+TGG 0.612177 6_4:+7860116 Msa0974640:three_prime_UTR
TTTGGGTTCCGAAAACGTTG+AGG 0.613827 6_4:+7859774 Msa0974640:CDS
CCAGAGATACTGGACTGCCG+GGG 0.615553 6_4:+7859013 Msa0974640:CDS
ATGCTATTACAACGGGGACA+CGG 0.617301 6_4:-7858924 None:intergenic
TTCGGCGTCCATGTTCAAGA+CGG 0.617829 6_4:-7860719 None:intergenic
TAAGGCGGACGCGATTCGTG+GGG 0.619512 6_4:+7859859 Msa0974640:CDS
TAAGCTTATGTGACATAGTG+AGG 0.620731 6_4:+7860417 Msa0974640:three_prime_UTR
CATGCCACTCTATCCTCCAC+CGG 0.623143 6_4:+7859559 Msa0974640:CDS
TAAGCCGGTGGAGGATAGAG+TGG 0.624963 6_4:-7859563 None:intergenic
CAGAGATACTGGACTGCCGG+GGG 0.627532 6_4:+7859014 Msa0974640:CDS
GCAAAAGCCAAGGGGCACCA+GGG 0.629020 6_4:-7859469 None:intergenic
TTCTTGAATTCAGGACACAA+TGG 0.631423 6_4:+7858686 Msa0974640:intron
GAGTAGTTTCACCCCATAGG+TGG 0.632609 6_4:+7860320 Msa0974640:three_prime_UTR
AGTGGCATGGCAAATCCTTG+TGG 0.633181 6_4:-7859545 None:intergenic
AGATTGCATTGATTCACACA+AGG 0.639343 6_4:-7859219 None:intergenic
GACTCAACAGGTTCTGACGA+AGG 0.640024 6_4:+7859728 Msa0974640:CDS
AGTGACACGTTATGTTTGTG+TGG 0.649491 6_4:-7857020 None:intergenic
GATGGTATCATTCTAAACCA+TGG 0.655530 6_4:+7857280 Msa0974640:CDS
AAGGCGGACGCGATTCGTGG+GGG 0.657854 6_4:+7859860 Msa0974640:CDS
CGGTGGAGGATAGAGTGGCA+TGG 0.658087 6_4:-7859558 None:intergenic
GTGCCATTACAGTTCAGTGA+AGG 0.658325 6_4:-7859170 None:intergenic
GATTGCATTGATTCACACAA+GGG 0.666221 6_4:-7859218 None:intergenic
CTATGAAACAGACACAACAT+TGG 0.667427 6_4:+7860068 Msa0974640:three_prime_UTR
ATCAATTAACCCCTCCACTG+AGG 0.675714 6_4:+7858809 Msa0974640:CDS
GGTAGTACGGAACTTGCGGT+GGG 0.677836 6_4:-7859448 None:intergenic
CTAAAACCCCAATAAGCCGG+TGG 0.682119 6_4:-7859575 None:intergenic
GCCATGAGAAATGTGCCTGT+AGG 0.725661 6_4:+7859038 Msa0974640:CDS
ATTCCACGGCAAAAGCCAAG+GGG 0.744240 6_4:-7859477 None:intergenic
GTGTCCATGCTATTACAACG+GGG 0.773351 6_4:-7858930 None:intergenic
GACATGCGTTACAAGCACAG+TGG 0.786778 6_4:+7860144 Msa0974640:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATATGATGAAAAAAG+TGG - chr6_4:7857077-7857096 None:intergenic 15.0%
!! AAAAAAAAAACACACATAAA+AGG - chr6_4:7857462-7857481 None:intergenic 15.0%
!!! ATCATAGTTTTTTTTTTGTT+TGG + chr6_4:7857718-7857737 Msa0974640:intron 15.0%
!! ATAAAGAAAAAACAGATTTA+AGG + chr6_4:7858166-7858185 Msa0974640:intron 15.0%
!!! TTTTCAGAATTTTATCATTT+CGG + chr6_4:7860528-7860547 Msa0974640:three_prime_UTR 15.0%
!! AAAAAGAAACAGAAAAATCA+TGG + chr6_4:7857141-7857160 Msa0974640:exon 20.0%
!!! TATTTTCACATTTGAAATCA+CGG - chr6_4:7857374-7857393 None:intergenic 20.0%
!! TCAAATGTGAAAATATTTAG+TGG + chr6_4:7857379-7857398 Msa0974640:intron 20.0%
!! CAAATGTGAAAATATTTAGT+GGG + chr6_4:7857380-7857399 Msa0974640:intron 20.0%
!!! TGTGTTTTTTTTTTGTTGAA+GGG + chr6_4:7857468-7857487 Msa0974640:intron 20.0%
!!! TTCAATTTTGGAATTGATTT+TGG + chr6_4:7857546-7857565 Msa0974640:intron 20.0%
!!! ATTTGATTCAAAGTTTTAAC+CGG + chr6_4:7857894-7857913 Msa0974640:intron 20.0%
!! ATTGAAAACTTCAAAGATTA+TGG + chr6_4:7858845-7858864 Msa0974640:CDS 20.0%
!! TTATAAGCTACATAATTATG+AGG - chr6_4:7859999-7860018 None:intergenic 20.0%
!!! TCATTTTCTCAAATCATTAA+TGG - chr6_4:7860110-7860129 None:intergenic 20.0%
!!! AGAAAACATTCTAAAAGAAA+GGG - chr6_4:7860378-7860397 None:intergenic 20.0%
!!! AAGAAAACATTCTAAAAGAA+AGG - chr6_4:7860379-7860398 None:intergenic 20.0%
!!! ATGGAAGTATTTTATCATTT+TGG + chr6_4:7860561-7860580 Msa0974640:three_prime_UTR 20.0%
!!! ATTTTCACATTTGAAATCAC+GGG - chr6_4:7857373-7857392 None:intergenic 25.0%
!!! GTGTGTTTTTTTTTTGTTGA+AGG + chr6_4:7857467-7857486 Msa0974640:intron 25.0%
!!! GTTTGGATTTTGTGTTTTTT+TGG + chr6_4:7857502-7857521 Msa0974640:intron 25.0%
!!! TTTTTTGGATCTATGTTGTT+TGG + chr6_4:7857517-7857536 Msa0974640:intron 25.0%
!!! GTTTGGTTTTGTTTCAATTT+TGG + chr6_4:7857534-7857553 Msa0974640:intron 25.0%
!!! GGAATTGATTTTGGTTTTTA+AGG + chr6_4:7857555-7857574 Msa0974640:intron 25.0%
! GCTTAAGCTTAACATTAAAT+TGG + chr6_4:7857628-7857647 Msa0974640:intron 25.0%
! CTAGAACTAAATCAAAACTA+GGG - chr6_4:7857749-7857768 None:intergenic 25.0%
! TCATAATAACGAATCTAAGA+TGG - chr6_4:7857807-7857826 None:intergenic 25.0%
! TTCAGAATCAGATATAGAAA+AGG - chr6_4:7858074-7858093 None:intergenic 25.0%
! AAAAACAGATTTAAGGAGTA+AGG + chr6_4:7858173-7858192 Msa0974640:intron 25.0%
!! ATCTTTTTCCTTCTTACATT+TGG - chr6_4:7858724-7858743 None:intergenic 25.0%
! TCTCGGGATTAAAAATAATA+AGG + chr6_4:7859841-7859860 Msa0974640:CDS 25.0%
!! ATTTGAGAAAATGAAAGTGT+TGG + chr6_4:7860116-7860135 Msa0974640:three_prime_UTR 25.0%
! TACAAACTGTAAGAATACAT+TGG + chr6_4:7860190-7860209 Msa0974640:three_prime_UTR 25.0%
!!! CATTGGTTCATTTTTGTTTT+TGG + chr6_4:7860207-7860226 Msa0974640:three_prime_UTR 25.0%
!!! AGTTAGTTTTTGTTGATCAA+TGG + chr6_4:7860234-7860253 Msa0974640:three_prime_UTR 25.0%
!! AAACTGTTTGATGTGTTTTT+AGG + chr6_4:7860693-7860712 Msa0974640:three_prime_UTR 25.0%
AGAAGAGAAAGAAATTGATG+AGG + chr6_4:7857318-7857337 Msa0974640:CDS 30.0%
! TGTGAAAATATTTAGTGGGT+TGG + chr6_4:7857384-7857403 Msa0974640:intron 30.0%
GAAGGGTTTAGTATTTAGTT+TGG + chr6_4:7857485-7857504 Msa0974640:intron 30.0%
GCTAGAACTAAATCAAAACT+AGG - chr6_4:7857750-7857769 None:intergenic 30.0%
ACGATATCTATACTGAAACT+TGG - chr6_4:7857842-7857861 None:intergenic 30.0%
!! GTCAAACAAAGTTGATGTAT+TGG - chr6_4:7857949-7857968 None:intergenic 30.0%
ATAAACTCTATTTACTCCTC+TGG - chr6_4:7857971-7857990 None:intergenic 30.0%
AACACAATCAATCAATACTC+TGG - chr6_4:7858024-7858043 None:intergenic 30.0%
! ATCTTTCTCTATTTAGCTTC+TGG + chr6_4:7858120-7858139 Msa0974640:intron 30.0%
! TCTTTCTCTATTTAGCTTCT+GGG + chr6_4:7858121-7858140 Msa0974640:intron 30.0%
!!! TTTTGTCATACTTTCTGGTT+TGG + chr6_4:7858222-7858241 Msa0974640:intron 30.0%
! TGACTATAGACAAAAGTACA+AGG - chr6_4:7858258-7858277 None:intergenic 30.0%
CATAAACACTTGAGACTATT+CGG + chr6_4:7858433-7858452 Msa0974640:intron 30.0%
! TCAAGAGTTTTCTTGAATTC+AGG + chr6_4:7858677-7858696 Msa0974640:intron 30.0%
!!! TATTTTGTCTGGCTTTTTCA+GGG - chr6_4:7858901-7858920 None:intergenic 30.0%
GTTATTGTAGTAGCAGAATT+TGG - chr6_4:7858955-7858974 None:intergenic 30.0%
TATTAACTCCTTTATCAGCA+AGG - chr6_4:7859253-7859272 None:intergenic 30.0%
GTTAATATTTCCGCGAAAAA+CGG + chr6_4:7859266-7859285 Msa0974640:CDS 30.0%
TGATCATTCCATCAAATCTT+CGG + chr6_4:7859349-7859368 Msa0974640:CDS 30.0%
ATAAAGAAGAAAAGTCCCTT+TGG + chr6_4:7859756-7859775 Msa0974640:CDS 30.0%
TAAAGAAGAAAAGTCCCTTT+GGG + chr6_4:7859757-7859776 Msa0974640:CDS 30.0%
CGGGATTAAAAATAATAAGG+CGG + chr6_4:7859844-7859863 Msa0974640:CDS 30.0%
AATGGAAGATATTGAGAAGA+TGG + chr6_4:7860252-7860271 Msa0974640:three_prime_UTR 30.0%
TAATCATGTAAACGTGACTT+TGG + chr6_4:7860623-7860642 Msa0974640:three_prime_UTR 30.0%
AAACAAACGATTGTTTGGTT+CGG - chr6_4:7860740-7860759 None:intergenic 30.0%
CAAACAAACAAACGATTGTT+TGG - chr6_4:7860745-7860764 None:intergenic 30.0%
GATGGTATCATTCTAAACCA+TGG + chr6_4:7857280-7857299 Msa0974640:CDS 35.0%
TTCTTTCTCTTCTTCATCCA+TGG - chr6_4:7857311-7857330 None:intergenic 35.0%
GATGCTGTTGTAGTTTCTAA+TGG + chr6_4:7857606-7857625 Msa0974640:intron 35.0%
! GCTCATTTTGTCATACTTTC+TGG + chr6_4:7858217-7858236 Msa0974640:intron 35.0%
TTGTCTATAGTCAGCTAACA+TGG + chr6_4:7858265-7858284 Msa0974640:intron 35.0%
!! GCTATAGTACTGATTATCTG+TGG + chr6_4:7858294-7858313 Msa0974640:intron 35.0%
AATTAGCGCTTAATGCGTAA+AGG - chr6_4:7858615-7858634 None:intergenic 35.0%
TTCTTGAATTCAGGACACAA+TGG + chr6_4:7858686-7858705 Msa0974640:intron 35.0%
TGAATCGACCAAATGTAAGA+AGG + chr6_4:7858713-7858732 Msa0974640:CDS 35.0%
! AGTGACCTTTTCTGATGATT+GGG - chr6_4:7858757-7858776 None:intergenic 35.0%
AGACACAAACTCAAGATTAG+TGG + chr6_4:7858782-7858801 Msa0974640:CDS 35.0%
!!! GTATTTTGTCTGGCTTTTTC+AGG - chr6_4:7858902-7858921 None:intergenic 35.0%
TCTGGCATTTCTTGCAAAAA+TGG - chr6_4:7858998-7859017 None:intergenic 35.0%
AGAATTCTGAACAGCTGTTT+CGG - chr6_4:7859120-7859139 None:intergenic 35.0%
!! GATTGCATTGATTCACACAA+GGG - chr6_4:7859221-7859240 None:intergenic 35.0%
!! AGATTGCATTGATTCACACA+AGG - chr6_4:7859222-7859241 None:intergenic 35.0%
!! TCTAATGATTCCGTTTTTCG+CGG - chr6_4:7859279-7859298 None:intergenic 35.0%
GAAAAACGGAATCATTAGAC+CGG + chr6_4:7859280-7859299 Msa0974640:CDS 35.0%
! CTCTTTTCTTCACCAACATA+GGG - chr6_4:7859326-7859345 None:intergenic 35.0%
! ACTCTTTTCTTCACCAACAT+AGG - chr6_4:7859327-7859346 None:intergenic 35.0%
AACCTTAGGTAAGCATTCAA+GGG + chr6_4:7859682-7859701 Msa0974640:CDS 35.0%
! TCATCAATCCTCAACGTTTT+CGG - chr6_4:7859785-7859804 None:intergenic 35.0%
AGCTCAATATTGACAACTCT+CGG + chr6_4:7859824-7859843 Msa0974640:CDS 35.0%
TTCATTGCTCTTTGAACCAA+AGG - chr6_4:7859900-7859919 None:intergenic 35.0%
CTATGAAACAGACACAACAT+TGG + chr6_4:7860068-7860087 Msa0974640:three_prime_UTR 35.0%
TTCTTTCTGCATTCGCTTTA+AGG + chr6_4:7860275-7860294 Msa0974640:three_prime_UTR 35.0%
TAAGCTTATGTGACATAGTG+AGG + chr6_4:7860417-7860436 Msa0974640:three_prime_UTR 35.0%
! TCATTTCGGTGCAATAAACA+TGG + chr6_4:7860542-7860561 Msa0974640:three_prime_UTR 35.0%
TGGATAACTATGCATGTGTT+TGG + chr6_4:7860581-7860600 Msa0974640:three_prime_UTR 35.0%
!! TGTTTGGTAGTGCATTACAT+TGG + chr6_4:7860597-7860616 Msa0974640:three_prime_UTR 35.0%
!! GCTTTTTGGGAAGACAATTC+CGG + chr6_4:7857186-7857205 Msa0974640:CDS 40.0%
TCTAAACCATGGTTCTTCCA+TGG + chr6_4:7857291-7857310 Msa0974640:CDS 40.0%
!!! AGTGGGTTGGTGTTAGATTT+TGG + chr6_4:7857397-7857416 Msa0974640:intron 40.0%
! CATCAACTTTGTTTGACCAG+AGG + chr6_4:7857952-7857971 Msa0974640:intron 40.0%
!! AGCTTCTGGGTTTTGTATGT+TGG + chr6_4:7858134-7858153 Msa0974640:intron 40.0%
TCTCACCCAATCATCAGAAA+AGG + chr6_4:7858749-7858768 Msa0974640:CDS 40.0%
! CAGTGACCTTTTCTGATGAT+TGG - chr6_4:7858758-7858777 None:intergenic 40.0%
TGGTGTCCATGCTATTACAA+CGG - chr6_4:7858935-7858954 None:intergenic 40.0%
CATTTCTTGCAAAAATGGCG+AGG - chr6_4:7858993-7859012 None:intergenic 40.0%
ATTCTTGTTCTTCCTACGAC+CGG - chr6_4:7859066-7859085 None:intergenic 40.0%
! CTGTTTCGGGTACAGTTATT+TGG - chr6_4:7859106-7859125 None:intergenic 40.0%
! GAATTCTGAACAGCTGTTTC+GGG - chr6_4:7859119-7859138 None:intergenic 40.0%
TACAGTTCAGTGAAGGATGA+TGG - chr6_4:7859166-7859185 None:intergenic 40.0%
CATCCTTCACTGAACTGTAA+TGG + chr6_4:7859167-7859186 Msa0974640:CDS 40.0%
ATGGCACAGTCTTTACATTC+AGG + chr6_4:7859186-7859205 Msa0974640:CDS 40.0%
GCATTGAACCTTGCTGATAA+AGG + chr6_4:7859242-7859261 Msa0974640:CDS 40.0%
TGATGTGTCCGAAGATTTGA+TGG - chr6_4:7859360-7859379 None:intergenic 40.0%
! AACTTGTTCAAGAGAACTGC+TGG - chr6_4:7859408-7859427 None:intergenic 40.0%
AATCCTTGTGGGAAAAAAGC+AGG - chr6_4:7859536-7859555 None:intergenic 40.0%
ATGCTAAAACCCCAATAAGC+CGG - chr6_4:7859581-7859600 None:intergenic 40.0%
CTTGAATGCTTACCTAAGGT+TGG - chr6_4:7859683-7859702 None:intergenic 40.0%
CAACCTTAGGTAAGCATTCA+AGG + chr6_4:7859681-7859700 Msa0974640:CDS 40.0%
TTCCCTTGAATGCTTACCTA+AGG - chr6_4:7859687-7859706 None:intergenic 40.0%
! ACGTTGAGGATTGATGACTT+AGG + chr6_4:7859788-7859807 Msa0974640:CDS 40.0%
GCTCAATATTGACAACTCTC+GGG + chr6_4:7859825-7859844 Msa0974640:CDS 40.0%
TTCATAGCTGCTGTGTCAAT+TGG - chr6_4:7860055-7860074 None:intergenic 40.0%
ATATACATCGACGATCTTGC+AGG - chr6_4:7860460-7860479 None:intergenic 40.0%
CATGGTAGCTTTCATGTTAC+AGG + chr6_4:7860657-7860676 Msa0974640:three_prime_UTR 40.0%
ATCATGGAAGCAAAAGACCC+TGG + chr6_4:7857157-7857176 Msa0974640:exon 45.0%
TCATGGAAGCAAAAGACCCT+GGG + chr6_4:7857158-7857177 Msa0974640:exon 45.0%
! ACCCTGGGATCAAGCTTTTT+GGG + chr6_4:7857173-7857192 Msa0974640:CDS 45.0%
TCCCAAAAAGCTTGATCCCA+GGG - chr6_4:7857177-7857196 None:intergenic 45.0%
TTCCCAAAAAGCTTGATCCC+AGG - chr6_4:7857178-7857197 None:intergenic 45.0%
! TCTGGTGATGCTGTTGATGA+TGG + chr6_4:7857262-7857281 Msa0974640:CDS 45.0%
CTTCATCCATGGAAGAACCA+TGG - chr6_4:7857300-7857319 None:intergenic 45.0%
!! TAACCGGAAGTTGATGTGTC+TGG + chr6_4:7857910-7857929 Msa0974640:intron 45.0%
GAACCAGACACATCAACTTC+CGG - chr6_4:7857916-7857935 None:intergenic 45.0%
ATCAATTAACCCCTCCACTG+AGG + chr6_4:7858809-7858828 Msa0974640:CDS 45.0%
! TGTATTTTGCTCGTCCTCAG+TGG - chr6_4:7858826-7858845 None:intergenic 45.0%
ATGCTATTACAACGGGGACA+CGG - chr6_4:7858927-7858946 None:intergenic 45.0%
GTGTCCATGCTATTACAACG+GGG - chr6_4:7858933-7858952 None:intergenic 45.0%
GGTGTCCATGCTATTACAAC+GGG - chr6_4:7858934-7858953 None:intergenic 45.0%
GCAAGAAATGCCAGAGATAC+TGG + chr6_4:7859003-7859022 Msa0974640:CDS 45.0%
ACCTACAGGCACATTTCTCA+TGG - chr6_4:7859042-7859061 None:intergenic 45.0%
! GTGCCATTACAGTTCAGTGA+AGG - chr6_4:7859173-7859192 None:intergenic 45.0%
AACGGAATCATTAGACCGGA+AGG + chr6_4:7859284-7859303 Msa0974640:CDS 45.0%
TCATTAGACCGGAAGGTCTA+CGG + chr6_4:7859291-7859310 Msa0974640:CDS 45.0%
ACATGAATCCGTAGACCTTC+CGG - chr6_4:7859302-7859321 None:intergenic 45.0%
CGGATTCATGTACCCTATGT+TGG + chr6_4:7859311-7859330 Msa0974640:CDS 45.0%
! GGTGGGAAGCTTTGACAATT+TGG - chr6_4:7859434-7859453 None:intergenic 45.0%
! CTCCATTGAGATGGATTCCA+CGG - chr6_4:7859494-7859513 None:intergenic 45.0%
ACTTGAGAGCTCCATTGAGA+TGG - chr6_4:7859503-7859522 None:intergenic 45.0%
!! TATTGGGGTTTTAGCATGCC+CGG + chr6_4:7859584-7859603 Msa0974640:CDS 45.0%
! GCTGCACTATTTGGTGAAGA+AGG - chr6_4:7859638-7859657 None:intergenic 45.0%
GAGTTAACGGCTGCACTATT+TGG - chr6_4:7859647-7859666 None:intergenic 45.0%
AATAGTGCAGCCGTTAACTC+CGG + chr6_4:7859647-7859666 Msa0974640:CDS 45.0%
TTACCTAAGGTTGGCGAGTT+AGG - chr6_4:7859674-7859693 None:intergenic 45.0%
TTCGTCAGAACCTGTTGAGT+CGG - chr6_4:7859729-7859748 None:intergenic 45.0%
! CAACGTTTTCGGAACCCAAA+GGG - chr6_4:7859774-7859793 None:intergenic 45.0%
! TCAACGTTTTCGGAACCCAA+AGG - chr6_4:7859775-7859794 None:intergenic 45.0%
! TTTGGGTTCCGAAAACGTTG+AGG + chr6_4:7859774-7859793 Msa0974640:CDS 45.0%
! GGAGGACTTTTCAAAGCCTT+TGG + chr6_4:7859881-7859900 Msa0974640:CDS 45.0%
ATTGACCTAGACATTGCTGC+TGG - chr6_4:7859962-7859981 None:intergenic 45.0%
! ACTTTGGAGAAGCAGCTACA+TGG + chr6_4:7860639-7860658 Msa0974640:three_prime_UTR 45.0%
!!! TTTTATTTTTATGTATTTTT+TGG - chr6_4:7857119-7857138 None:intergenic 5.0%
! GACCCTGGGATCAAGCTTTT+TGG + chr6_4:7857172-7857191 Msa0974640:CDS 50.0%
TGGGAAGACAATTCCGGTAC+CGG + chr6_4:7857192-7857211 Msa0974640:CDS 50.0%
AGAAGATGAAGCTGGTGCTC+CGG - chr6_4:7857245-7857264 None:intergenic 50.0%
GCATCACCAGAAGATGAAGC+TGG - chr6_4:7857253-7857272 None:intergenic 50.0%
! TTTTGCTCGTCCTCAGTGGA+GGG - chr6_4:7858822-7858841 None:intergenic 50.0%
! ATTTTGCTCGTCCTCAGTGG+AGG - chr6_4:7858823-7858842 None:intergenic 50.0%
! GGACACGGCAGTATTTTGTC+TGG - chr6_4:7858912-7858931 None:intergenic 50.0%
GTGTCCCCGTTGTAATAGCA+TGG + chr6_4:7858926-7858945 Msa0974640:CDS 50.0%
TCTTGCAAAAATGGCGAGGC+TGG - chr6_4:7858989-7859008 None:intergenic 50.0%
GCCATGAGAAATGTGCCTGT+AGG + chr6_4:7859038-7859057 Msa0974640:CDS 50.0%
AGAAATGTGCCTGTAGGTGC+CGG + chr6_4:7859044-7859063 Msa0974640:CDS 50.0%
! TCTCAATCCGACTCGCCAAA+CGG + chr6_4:7859137-7859156 Msa0974640:CDS 50.0%
!! GAAGGATGATGGACTCCGTT+TGG - chr6_4:7859155-7859174 None:intergenic 50.0%
ATTCCACGGCAAAAGCCAAG+GGG - chr6_4:7859480-7859499 None:intergenic 50.0%
GATTCCACGGCAAAAGCCAA+GGG - chr6_4:7859481-7859500 None:intergenic 50.0%
TGCCGTGGAATCCATCTCAA+TGG + chr6_4:7859489-7859508 Msa0974640:CDS 50.0%
TGTGGGAAAAAAGCAGGTGG+TGG - chr6_4:7859530-7859549 None:intergenic 50.0%
CCTTGTGGGAAAAAAGCAGG+TGG - chr6_4:7859533-7859552 None:intergenic 50.0%
!!! CCACCTGCTTTTTTCCCACA+AGG + chr6_4:7859530-7859549 Msa0974640:CDS 50.0%
GTGGCATGGCAAATCCTTGT+GGG - chr6_4:7859547-7859566 None:intergenic 50.0%
AGTGGCATGGCAAATCCTTG+TGG - chr6_4:7859548-7859567 None:intergenic 50.0%
!! TCTATCCTCCACCGGCTTAT+TGG + chr6_4:7859567-7859586 Msa0974640:CDS 50.0%
!! CTATCCTCCACCGGCTTATT+GGG + chr6_4:7859568-7859587 Msa0974640:CDS 50.0%
!! TATCCTCCACCGGCTTATTG+GGG + chr6_4:7859569-7859588 Msa0974640:CDS 50.0%
CTAAAACCCCAATAAGCCGG+TGG - chr6_4:7859578-7859597 None:intergenic 50.0%
CGAGTTAGGACCGGAGTTAA+CGG - chr6_4:7859660-7859679 None:intergenic 50.0%
TAAGGTTGGCGAGTTAGGAC+CGG - chr6_4:7859669-7859688 None:intergenic 50.0%
! CTCAAACCAACCGACTCAAC+AGG + chr6_4:7859716-7859735 Msa0974640:CDS 50.0%
TCAGAACCTGTTGAGTCGGT+TGG - chr6_4:7859725-7859744 None:intergenic 50.0%
! GACTCAACAGGTTCTGACGA+AGG + chr6_4:7859728-7859747 Msa0974640:CDS 50.0%
CCTAGACATTGCTGCTGGAT+TGG - chr6_4:7859957-7859976 None:intergenic 50.0%
CCAATCCAGCAGCAATGTCT+AGG + chr6_4:7859954-7859973 Msa0974640:CDS 50.0%
GACATGCGTTACAAGCACAG+TGG + chr6_4:7860144-7860163 Msa0974640:three_prime_UTR 50.0%
GCAGAGTAGTTTCACCCCAT+AGG + chr6_4:7860317-7860336 Msa0974640:three_prime_UTR 50.0%
GAGTAGTTTCACCCCATAGG+TGG + chr6_4:7860320-7860339 Msa0974640:three_prime_UTR 50.0%
TAGTTTCACCCCATAGGTGG+CGG + chr6_4:7860323-7860342 Msa0974640:three_prime_UTR 50.0%
TTAGGACGCCGTCTTGAACA+TGG + chr6_4:7860711-7860730 Msa0974640:three_prime_UTR 50.0%
TTCGGCGTCCATGTTCAAGA+CGG - chr6_4:7860722-7860741 None:intergenic 50.0%
! GGAGCACCAGCTTCATCTTC+TGG + chr6_4:7857244-7857263 Msa0974640:CDS 55.0%
TTTGCTCGTCCTCAGTGGAG+GGG - chr6_4:7858821-7858840 None:intergenic 55.0%
TGCCAGAGATACTGGACTGC+CGG + chr6_4:7859011-7859030 Msa0974640:CDS 55.0%
CACATTTCTCATGGCTCCCC+CGG - chr6_4:7859033-7859052 None:intergenic 55.0%
ATGGACTCCGTTTGGCGAGT+CGG - chr6_4:7859147-7859166 None:intergenic 55.0%
GGTAGTACGGAACTTGCGGT+GGG - chr6_4:7859451-7859470 None:intergenic 55.0%
CCAGGGTAGTACGGAACTTG+CGG - chr6_4:7859455-7859474 None:intergenic 55.0%
GGATTCCACGGCAAAAGCCA+AGG - chr6_4:7859482-7859501 None:intergenic 55.0%
CATGCCACTCTATCCTCCAC+CGG + chr6_4:7859559-7859578 Msa0974640:CDS 55.0%
TAAGCCGGTGGAGGATAGAG+TGG - chr6_4:7859566-7859585 None:intergenic 55.0%
AAACCCCAATAAGCCGGTGG+AGG - chr6_4:7859575-7859594 None:intergenic 55.0%
! CATGGATTGTTCCACGCACC+GGG - chr6_4:7859605-7859624 None:intergenic 55.0%
! CCATGGATTGTTCCACGCAC+CGG - chr6_4:7859606-7859625 None:intergenic 55.0%
TGCGTGGAACAATCCATGGC+TGG + chr6_4:7859607-7859626 Msa0974640:CDS 55.0%
GAAGAAGGTTGTGCCAGCCA+TGG - chr6_4:7859623-7859642 None:intergenic 55.0%
GGTCCTAACTCGCCAACCTT+AGG + chr6_4:7859668-7859687 Msa0974640:CDS 55.0%
AATAAGGCGGACGCGATTCG+TGG + chr6_4:7859857-7859876 Msa0974640:CDS 55.0%
ATAAGGCGGACGCGATTCGT+GGG + chr6_4:7859858-7859877 Msa0974640:CDS 55.0%
GCAAAACTCACCTGCCATGC+AGG + chr6_4:7859931-7859950 Msa0974640:CDS 55.0%
TCCGGTAGAGTCACCGGATC+CGG - chr6_4:7857227-7857246 None:intergenic 60.0%
TGGTGCTCCGGTAGAGTCAC+CGG - chr6_4:7857233-7857252 None:intergenic 60.0%
GCCAGAGATACTGGACTGCC+GGG + chr6_4:7859012-7859031 Msa0974640:CDS 60.0%
CCAGAGATACTGGACTGCCG+GGG + chr6_4:7859013-7859032 Msa0974640:CDS 60.0%
CAGAGATACTGGACTGCCGG+GGG + chr6_4:7859014-7859033 Msa0974640:CDS 60.0%
TTCCTACGACCGGCACCTAC+AGG - chr6_4:7859056-7859075 None:intergenic 60.0%
GGGTAGTACGGAACTTGCGG+TGG - chr6_4:7859452-7859471 None:intergenic 60.0%
CCGCAAGTTCCGTACTACCC+TGG + chr6_4:7859452-7859471 Msa0974640:CDS 60.0%
!! CAAGGGGCACCAGGGTAGTA+CGG - chr6_4:7859464-7859483 None:intergenic 60.0%
!! GCAAAAGCCAAGGGGCACCA+GGG - chr6_4:7859472-7859491 None:intergenic 60.0%
CGGTGGAGGATAGAGTGGCA+TGG - chr6_4:7859561-7859580 None:intergenic 60.0%
!! GTTTTAGCATGCCCGGTGCG+TGG + chr6_4:7859591-7859610 Msa0974640:CDS 60.0%
CCGGTGCGTGGAACAATCCA+TGG + chr6_4:7859603-7859622 Msa0974640:CDS 60.0%
TAAGGCGGACGCGATTCGTG+GGG + chr6_4:7859859-7859878 Msa0974640:CDS 60.0%
!! TGCTGCTGGATTGGCCTGCA+TGG - chr6_4:7859948-7859967 None:intergenic 60.0%
! TTTCACCCCATAGGTGGCGG+CGG + chr6_4:7860326-7860345 Msa0974640:three_prime_UTR 60.0%
CCGGCAGAGATCTCCGGTAC+CGG - chr6_4:7857208-7857227 None:intergenic 65.0%
! CCGGTACCGGAGATCTCTGC+CGG + chr6_4:7857205-7857224 Msa0974640:CDS 65.0%
CCGGATCCGGCAGAGATCTC+CGG - chr6_4:7857214-7857233 None:intergenic 65.0%
CCGGAGATCTCTGCCGGATC+CGG + chr6_4:7857211-7857230 Msa0974640:CDS 65.0%
GCCGGATCCGGTGACTCTAC+CGG + chr6_4:7857223-7857242 Msa0974640:CDS 65.0%
CCCCGGCAGTCCAGTATCTC+TGG - chr6_4:7859016-7859035 None:intergenic 65.0%
!! TGCCTGTAGGTGCCGGTCGT+AGG + chr6_4:7859051-7859070 Msa0974640:CDS 65.0%
CGTACTACCCTGGTGCCCCT+TGG + chr6_4:7859462-7859481 Msa0974640:CDS 65.0%
!! GGCAAAAGCCAAGGGGCACC+AGG - chr6_4:7859473-7859492 None:intergenic 65.0%
!! GTGCCCCTTGGCTTTTGCCG+TGG + chr6_4:7859474-7859493 Msa0974640:CDS 65.0%
AAGGCGGACGCGATTCGTGG+GGG + chr6_4:7859860-7859879 Msa0974640:CDS 65.0%
!! GCTGGATTGGCCTGCATGGC+AGG - chr6_4:7859944-7859963 None:intergenic 65.0%
CGCAACCGCCGCCACCTATG+GGG - chr6_4:7860334-7860353 None:intergenic 70.0%
CCGCAACCGCCGCCACCTAT+GGG - chr6_4:7860335-7860354 None:intergenic 70.0%
! CCCATAGGTGGCGGCGGTTG+CGG + chr6_4:7860332-7860351 Msa0974640:three_prime_UTR 70.0%
GCGGACGCGATTCGTGGGGG+AGG + chr6_4:7859863-7859882 Msa0974640:CDS 75.0%
GCCGCAACCGCCGCCACCTA+TGG - chr6_4:7860336-7860355 None:intergenic 75.0%
! AGGTGGCGGCGGTTGCGGCA+AGG + chr6_4:7860337-7860356 Msa0974640:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 7857023 7860792 7857023 ID=Msa0974640;Name=Msa0974640
chr6_4 mRNA 7857023 7860792 7857023 ID=Msa0974640-mRNA-1;Parent=Msa0974640;Name=Msa0974640-mRNA-1;_AED=0.00;_eAED=0.00;_QI=137|1|1|1|1|1|2|789|494
chr6_4 exon 7857023 7857339 7857023 ID=Msa0974640-mRNA-1:exon:2953;Parent=Msa0974640-mRNA-1
chr6_4 exon 7858699 7860792 7858699 ID=Msa0974640-mRNA-1:exon:2954;Parent=Msa0974640-mRNA-1
chr6_4 five_prime_UTR 7857023 7857159 7857023 ID=Msa0974640-mRNA-1:five_prime_utr;Parent=Msa0974640-mRNA-1
chr6_4 CDS 7857160 7857339 7857160 ID=Msa0974640-mRNA-1:cds;Parent=Msa0974640-mRNA-1
chr6_4 CDS 7858699 7860003 7858699 ID=Msa0974640-mRNA-1:cds;Parent=Msa0974640-mRNA-1
chr6_4 three_prime_UTR 7860004 7860792 7860004 ID=Msa0974640-mRNA-1:three_prime_utr;Parent=Msa0974640-mRNA-1
Gene Sequence

>Msa0974640

ATGGAAGCAAAAGACCCTGGGATCAAGCTTTTTGGGAAGACAATTCCGGTACCGGAGATCTCTGCCGGATCCGGTGACTCTACCGGAGCACCAGCTTCATCTTCTGGTGATGCTGTTGATGATGGTATCATTCTAAACCATGGTTCTTCCATGGATGAAGAAGAGAAAGAAATTGATGAGGACACAATGGAAGATGAATCGACCAAATGTAAGAAGGAAAAAGATGAAGCTCTCACCCAATCATCAGAAAAGGTCACTGATCTAGACACAAACTCAAGATTAGTGGAAGAATCAATTAACCCCTCCACTGAGGACGAGCAAAATACATTGAAAACTTCAAAGATTATGGAAGAACATACTGAGAATTCTCATGATAAGACCCTGAAAAAGCCAGACAAAATACTGCCGTGTCCCCGTTGTAATAGCATGGACACCAAATTCTGCTACTACAATAACTACAATGTTAACCAGCCTCGCCATTTTTGCAAGAAATGCCAGAGATACTGGACTGCCGGGGGAGCCATGAGAAATGTGCCTGTAGGTGCCGGTCGTAGGAAGAACAAGAATTCTGCTTCTCACTTTCGCCAAATAACTGTACCCGAAACAGCTGTTCAGAATTCTCAATCCGACTCGCCAAACGGAGTCCATCATCCTTCACTGAACTGTAATGGCACAGTCTTTACATTCAGGACTGATACACCCTTGTGTGAATCAATGCAATCTGCATTGAACCTTGCTGATAAAGGAGTTAATATTTCCGCGAAAAACGGAATCATTAGACCGGAAGGTCTACGGATTCATGTACCCTATGTTGGTGAAGAAAAGAGTGATGATCATTCCATCAAATCTTCGGACACATCAACAGCTCTGACAGAAGATGCAGCTGCCAGCAGTTCTCTTGAACAAGTTATGCCAAATTGTCAAAGCTTCCCACCGCAAGTTCCGTACTACCCTGGTGCCCCTTGGCTTTTGCCGTGGAATCCATCTCAATGGAGCTCTCAAGTACAACCACCACCTGCTTTTTTCCCACAAGGATTTGCCATGCCACTCTATCCTCCACCGGCTTATTGGGGTTTTAGCATGCCCGGTGCGTGGAACAATCCATGGCTGGCACAACCTTCTTCACCAAATAGTGCAGCCGTTAACTCCGGTCCTAACTCGCCAACCTTAGGTAAGCATTCAAGGGAAGAAAGCATGCTCAAACCAACCGACTCAACAGGTTCTGACGAAGGAAACAATAAAGAAGAAAAGTCCCTTTGGGTTCCGAAAACGTTGAGGATTGATGACTTAGGAGAAGCAGAAAAAAGCTCAATATTGACAACTCTCGGGATTAAAAATAATAAGGCGGACGCGATTCGTGGGGGAGGACTTTTCAAAGCCTTTGGTTCAAAGAGCAATGAAAAAGATAGCGTGCAAAACTCACCTGCCATGCAGGCCAATCCAGCAGCAATGTCTAGGTCAATTAGCTTTCATGAGACCTCATAA
Protein sequence

>Msa0974640

MEAKDPGIKLFGKTIPVPEISAGSGDSTGAPASSSGDAVDDGIILNHGSSMDEEEKEIDEDTMEDESTKCKKEKDEALTQSSEKVTDLDTNSRLVEESINPSTEDEQNTLKTSKIMEEHTENSHDKTLKKPDKILPCPRCNSMDTKFCYYNNYNVNQPRHFCKKCQRYWTAGGAMRNVPVGAGRRKNKNSASHFRQITVPETAVQNSQSDSPNGVHHPSLNCNGTVFTFRTDTPLCESMQSALNLADKGVNISAKNGIIRPEGLRIHVPYVGEEKSDDHSIKSSDTSTALTEDAAASSSLEQVMPNCQSFPPQVPYYPGAPWLLPWNPSQWSSQVQPPPAFFPQGFAMPLYPPPAYWGFSMPGAWNNPWLAQPSSPNSAAVNSGPNSPTLGKHSREESMLKPTDSTGSDEGNNKEEKSLWVPKTLRIDDLGEAEKSSILTTLGIKNNKADAIRGGGLFKAFGSKSNEKDSVQNSPAMQANPAAMSRSISFHETS*