AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0989280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0989280 MtrunA17_Chr8g0387591 92.771 83 6 0 9 91 862 944 1.18e-48 164
Msa0989280 MtrunA17_Chr2g0301511 63.855 83 30 0 9 91 849 931 1.32e-32 118
Msa0989280 MtrunA17_Chr7g0272591 60.241 83 33 0 9 91 813 895 3.59e-28 105
Msa0989280 MtrunA17_Chr3g0131401 54.217 83 38 0 9 91 841 923 1.35e-27 104
Msa0989280 MtrunA17_Chr7g0238371 56.627 83 36 0 9 91 769 851 2.08e-27 103
Msa0989280 MtrunA17_Chr1g0192121 57.831 83 35 0 9 91 803 885 3.49e-27 103
Msa0989280 MtrunA17_Chr8g0385411 50.602 83 41 0 9 91 816 898 7.99e-26 99.4
Msa0989280 MtrunA17_Chr4g0061441 50.562 89 44 0 3 91 742 830 1.57e-25 98.6
Msa0989280 MtrunA17_Chr3g0131381 50.562 89 44 0 3 91 829 917 1.49e-24 95.9
Msa0989280 MtrunA17_Chr3g0131361 48.193 83 43 0 9 91 828 910 3.28e-24 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0989280 AT1G64060.1 75.904 83 20 0 9 91 862 944 1.22e-37 133
Msa0989280 AT1G19230.2 61.446 83 32 0 9 91 870 952 3.03e-31 115
Msa0989280 AT4G11230.1 61.446 83 32 0 9 91 859 941 1.56e-30 113
Msa0989280 AT1G19230.1 61.446 83 32 0 9 91 844 926 3.53e-30 112
Msa0989280 AT5G60010.1 59.036 83 34 0 9 91 804 886 7.39e-28 105
Msa0989280 AT5G07390.1 57.831 83 35 0 9 91 820 902 7.61e-28 105
Msa0989280 AT1G09090.2 53.933 89 41 0 3 91 755 843 9.58e-28 105
Msa0989280 AT4G25090.1 52.809 89 42 0 3 91 761 849 1.08e-27 105
Msa0989280 AT4G25090.2 52.809 89 42 0 3 91 749 837 1.20e-27 104
Msa0989280 AT3G45810.1 57.831 83 35 0 9 91 830 912 1.36e-27 104
Msa0989280 AT5G47910.1 51.685 89 43 0 3 91 833 921 1.61e-27 104
Msa0989280 AT5G51060.1 54.217 83 38 0 9 91 823 905 6.33e-27 102
Msa0989280 AT5G07390.2 60.000 50 20 0 9 58 788 837 2.97e-16 72.4

Find 61 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGAGTTGATATTGTTTC+TGG 0.098642 6_4:+45619042 Msa0989280:CDS
AGGAGCATTTCTAACTAATT+TGG 0.161404 6_4:+45619471 Msa0989280:exon
ATTGAAGACATTGACTTAAT+TGG 0.204361 6_4:+45619690 Msa0989280:three_prime_UTR
TTTAGTTGTGTGTGATTTAT+AGG 0.216820 6_4:+45618757 None:intergenic
TGCTAGGCCTAATTGGAAAA+AGG 0.254620 6_4:+45619206 Msa0989280:CDS
ATAAATTAAAGAAAGATAAA+AGG 0.300162 6_4:+45619771 Msa0989280:three_prime_UTR
TAGAAACATAGGGCTGAAAA+TGG 0.323810 6_4:+45619873 Msa0989280:three_prime_UTR
TTAGTTGTGTGTGATTTATA+GGG 0.324918 6_4:+45618758 None:intergenic
AGGTAAGGGGACAAGGGATT+TGG 0.329052 6_4:+45619794 Msa0989280:three_prime_UTR
GCCTAGTTTATTAAGTTCTT+CGG 0.389157 6_4:-45619405 None:intergenic
CCACATCAATTTGCTATAAC+GGG 0.396873 6_4:-45619506 None:intergenic
GCCACATCAATTTGCTATAA+CGG 0.402560 6_4:-45619507 None:intergenic
AAATTAAACAAATGAGTTGA+TGG 0.402688 6_4:+45620175 Msa0989280:three_prime_UTR
AAGAAAGATAAAAGGAGGTA+AGG 0.405131 6_4:+45619779 Msa0989280:three_prime_UTR
TAAAAGGAGGTAAGGGGACA+AGG 0.423961 6_4:+45619787 Msa0989280:three_prime_UTR
TTGAACAATATCATATAATC+AGG 0.425792 6_4:-45619529 None:intergenic
AGAAAGATAAAAGGAGGTAA+GGG 0.425927 6_4:+45619780 Msa0989280:three_prime_UTR
AACTATGGTGATTGGTGATA+AGG 0.435945 6_4:-45618998 None:intergenic
AATGTTTGTAACGTATTGTA+TGG 0.446077 6_4:-45619670 None:intergenic
AGGCTTCGAGTTCAATGAAA+AGG 0.447822 6_4:+45619424 Msa0989280:CDS
CACGCTGGAATCGTCATGTA+AGG 0.450062 6_4:+45619901 Msa0989280:three_prime_UTR
TTGATATTGTTTCTGGAACC+AGG 0.454779 6_4:+45619049 Msa0989280:CDS
ATGTGTTCCAAGCACTATAG+TGG 0.458446 6_4:+45619240 Msa0989280:CDS
AAGCACTATAGTGGACGAAT+AGG 0.465955 6_4:+45619249 Msa0989280:CDS
AGCTCTAGTCGTAACCTTGA+TGG 0.474185 6_4:-45620065 None:intergenic
GTTATGATACAAAAGGTAAA+GGG 0.483001 6_4:-45620090 None:intergenic
TGTTATGATACAAAAGGTAA+AGG 0.484107 6_4:-45620091 None:intergenic
CATATAGACCTTTCCTCTGA+GGG 0.489228 6_4:-45620008 None:intergenic
CTGAAAATGGTGACTCACGC+TGG 0.489758 6_4:+45619886 Msa0989280:three_prime_UTR
ATGGTTAAGTGCTTGAACTA+TGG 0.496161 6_4:-45619013 None:intergenic
AGTGCTTGAACTATGGTGAT+TGG 0.498314 6_4:-45619006 None:intergenic
AAAAGGAGGTAAGGGGACAA+GGG 0.508171 6_4:+45619788 Msa0989280:three_prime_UTR
GCCGAAGAACTTAATAAACT+AGG 0.510408 6_4:+45619404 Msa0989280:CDS
TATTCGTCCACTATAGTGCT+TGG 0.520509 6_4:-45619247 None:intergenic
AGTAGCTGAGCTTGATCAAA+GGG 0.520659 6_4:+45618830 Msa0989280:five_prime_UTR
ACTATGGTGATTGGTGATAA+GGG 0.528002 6_4:-45618997 None:intergenic
GGCTTCGAGTTCAATGAAAA+GGG 0.539977 6_4:+45619425 Msa0989280:CDS
AGGAATTTCCCTCAGAGGAA+AGG 0.541746 6_4:+45620000 Msa0989280:three_prime_UTR
AGATCTGCAGTGAATTGAAA+GGG 0.546340 6_4:+45619736 Msa0989280:three_prime_UTR
GAAAGGTCTATATGTAAATG+AGG 0.550021 6_4:+45620017 Msa0989280:three_prime_UTR
TAGATCTGCAGTGAATTGAA+AGG 0.554148 6_4:+45619735 Msa0989280:three_prime_UTR
TGATATTGTTTCTGGAACCA+GGG 0.555585 6_4:+45619050 Msa0989280:CDS
TATCAGACAGCAAACATGCT+TGG 0.560324 6_4:+45619597 Msa0989280:three_prime_UTR
GAAAGATAAAAGGAGGTAAG+GGG 0.564736 6_4:+45619781 Msa0989280:three_prime_UTR
AAGTAGCTGAGCTTGATCAA+AGG 0.565757 6_4:+45618829 Msa0989280:five_prime_UTR
TTCGGTTGAGAGACGGAGAG+AGG 0.567485 6_4:-45619949 None:intergenic
TATAGGGTTACAAGAGAGCA+AGG 0.574650 6_4:+45618774 Msa0989280:five_prime_UTR
AACTACTTAACTAGCGAGGA+AGG 0.576255 6_4:+45618960 Msa0989280:five_prime_UTR
ATAATGTGTTATGATACAAA+AGG 0.580464 6_4:-45620097 None:intergenic
ATCAGGGTTCGGTTGAGAGA+CGG 0.582724 6_4:-45619956 None:intergenic
TACTGAGGAATTTCCCTCAG+AGG 0.583254 6_4:+45619995 Msa0989280:three_prime_UTR
ACATATAGACCTTTCCTCTG+AGG 0.605452 6_4:-45620009 None:intergenic
AATTGAAAGGGACAGTTCTG+AGG 0.612563 6_4:+45619748 Msa0989280:three_prime_UTR
AATTAAAGAAAGATAAAAGG+AGG 0.614874 6_4:+45619774 Msa0989280:three_prime_UTR
TCGGTTGAGAGACGGAGAGA+GGG 0.618844 6_4:-45619948 None:intergenic
ACGCTGGAATCGTCATGTAA+GGG 0.629001 6_4:+45619902 Msa0989280:three_prime_UTR
TAGCTACAAAGAAGATGTAA+CGG 0.635905 6_4:+45619636 Msa0989280:three_prime_UTR
TTAGTTAGAAATGCTCCTTG+TGG 0.636876 6_4:-45619466 None:intergenic
CCCGTTATAGCAAATTGATG+TGG 0.642807 6_4:+45619506 Msa0989280:three_prime_UTR
AACAAAATTTGAGTTCCACA+AGG 0.668640 6_4:+45619451 Msa0989280:CDS
GCACAACTACTTAACTAGCG+AGG 0.693637 6_4:+45618956 Msa0989280:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTAAAGAAAGATAAA+AGG + chr6_4:45619771-45619790 Msa0989280:three_prime_UTR 10.0%
!! ATAAATTAAAGAAAGATAAA+AGG + chr6_4:45619771-45619790 Msa0989280:three_prime_UTR 10.0%
!! TAATATTGATCGTCAATTTA+TGG + chr6_4:45618912-45618931 Msa0989280:intron 20.0%
!!! CTATTTTAATTTACTCAATC+CGG - chr6_4:45619147-45619166 None:intergenic 20.0%
!!! CTTTTGAATTTCTAATTATC+AGG + chr6_4:45619170-45619189 Msa0989280:intron 20.0%
!! TTGAACAATATCATATAATC+AGG - chr6_4:45619532-45619551 None:intergenic 20.0%
!! TTTCACATTCTATACAAAAA+AGG - chr6_4:45619565-45619584 None:intergenic 20.0%
!! AATTAAAGAAAGATAAAAGG+AGG + chr6_4:45619774-45619793 Msa0989280:three_prime_UTR 20.0%
!! AATTTACTCAAAGAAAAATG+AGG - chr6_4:45619844-45619863 None:intergenic 20.0%
!!! TTCGATAATTTTAGAAACAT+AGG + chr6_4:45619862-45619881 Msa0989280:three_prime_UTR 20.0%
!!! TCGATAATTTTAGAAACATA+GGG + chr6_4:45619863-45619882 Msa0989280:three_prime_UTR 20.0%
!! ATAATGTGTTATGATACAAA+AGG - chr6_4:45620100-45620119 None:intergenic 20.0%
!!! TTTTGTATCATAACACATTA+TGG + chr6_4:45620099-45620118 Msa0989280:three_prime_UTR 20.0%
!!! AAATTAAACAAATGAGTTGA+TGG + chr6_4:45620175-45620194 Msa0989280:three_prime_UTR 20.0%
!! TAATATTGATCGTCAATTTA+TGG + chr6_4:45618912-45618931 Msa0989280:intron 20.0%
!!! CTATTTTAATTTACTCAATC+CGG - chr6_4:45619147-45619166 None:intergenic 20.0%
!!! CTTTTGAATTTCTAATTATC+AGG + chr6_4:45619170-45619189 Msa0989280:intron 20.0%
!! TTGAACAATATCATATAATC+AGG - chr6_4:45619532-45619551 None:intergenic 20.0%
!! TTTCACATTCTATACAAAAA+AGG - chr6_4:45619565-45619584 None:intergenic 20.0%
!! AATTAAAGAAAGATAAAAGG+AGG + chr6_4:45619774-45619793 Msa0989280:three_prime_UTR 20.0%
!! AATTTACTCAAAGAAAAATG+AGG - chr6_4:45619844-45619863 None:intergenic 20.0%
!!! TTCGATAATTTTAGAAACAT+AGG + chr6_4:45619862-45619881 Msa0989280:three_prime_UTR 20.0%
!!! TCGATAATTTTAGAAACATA+GGG + chr6_4:45619863-45619882 Msa0989280:three_prime_UTR 20.0%
!! ATAATGTGTTATGATACAAA+AGG - chr6_4:45620100-45620119 None:intergenic 20.0%
!!! TTTTGTATCATAACACATTA+TGG + chr6_4:45620099-45620118 Msa0989280:three_prime_UTR 20.0%
!!! AAATTAAACAAATGAGTTGA+TGG + chr6_4:45620175-45620194 Msa0989280:three_prime_UTR 20.0%
!! AACAATATCAACTCCATTTT+TGG - chr6_4:45619040-45619059 None:intergenic 25.0%
!!! TAGAAATTAAAAGAGCATCA+AGG - chr6_4:45619121-45619140 None:intergenic 25.0%
!!! GTATCAGGTGTATTTTATTT+TGG + chr6_4:45619368-45619387 Msa0989280:intron 25.0%
! AATGTTTGTAACGTATTGTA+TGG - chr6_4:45619673-45619692 None:intergenic 25.0%
!!! ATTGAAGACATTGACTTAAT+TGG + chr6_4:45619690-45619709 Msa0989280:three_prime_UTR 25.0%
!! TTTTCTTTTTTCTTCCATCA+AGG + chr6_4:45620051-45620070 Msa0989280:three_prime_UTR 25.0%
! GTTATGATACAAAAGGTAAA+GGG - chr6_4:45620093-45620112 None:intergenic 25.0%
! TGTTATGATACAAAAGGTAA+AGG - chr6_4:45620094-45620113 None:intergenic 25.0%
!! AACAATATCAACTCCATTTT+TGG - chr6_4:45619040-45619059 None:intergenic 25.0%
!!! TAGAAATTAAAAGAGCATCA+AGG - chr6_4:45619121-45619140 None:intergenic 25.0%
!!! GTATCAGGTGTATTTTATTT+TGG + chr6_4:45619368-45619387 Msa0989280:intron 25.0%
! AATGTTTGTAACGTATTGTA+TGG - chr6_4:45619673-45619692 None:intergenic 25.0%
!!! ATTGAAGACATTGACTTAAT+TGG + chr6_4:45619690-45619709 Msa0989280:three_prime_UTR 25.0%
!! TTTTCTTTTTTCTTCCATCA+AGG + chr6_4:45620051-45620070 Msa0989280:three_prime_UTR 25.0%
! GTTATGATACAAAAGGTAAA+GGG - chr6_4:45620093-45620112 None:intergenic 25.0%
! TGTTATGATACAAAAGGTAA+AGG - chr6_4:45620094-45620113 None:intergenic 25.0%
!! AGATATTTGCTACTTGCTAT+TGG + chr6_4:45618888-45618907 Msa0989280:intron 30.0%
!! AATGGAGTTGATATTGTTTC+TGG + chr6_4:45619042-45619061 Msa0989280:CDS 30.0%
!!! GCTCTTTTAATTTCTAAGTC+CGG + chr6_4:45619125-45619144 Msa0989280:intron 30.0%
! GAGAAAACCTTTTTCCAATT+AGG - chr6_4:45619216-45619235 None:intergenic 30.0%
AAGTACCAACATATACTTGA+TGG - chr6_4:45619282-45619301 None:intergenic 30.0%
ATATGTTGGTACTTCATACA+TGG + chr6_4:45619288-45619307 Msa0989280:intron 30.0%
GCCTAGTTTATTAAGTTCTT+CGG - chr6_4:45619408-45619427 None:intergenic 30.0%
! TTGTGGAACTCAAATTTTGT+TGG - chr6_4:45619452-45619471 None:intergenic 30.0%
! AACAAAATTTGAGTTCCACA+AGG + chr6_4:45619451-45619470 Msa0989280:CDS 30.0%
!! AGGAGCATTTCTAACTAATT+TGG + chr6_4:45619471-45619490 Msa0989280:exon 30.0%
TAGCTACAAAGAAGATGTAA+CGG + chr6_4:45619636-45619655 Msa0989280:three_prime_UTR 30.0%
AAGAAAGATAAAAGGAGGTA+AGG + chr6_4:45619779-45619798 Msa0989280:three_prime_UTR 30.0%
AGAAAGATAAAAGGAGGTAA+GGG + chr6_4:45619780-45619799 Msa0989280:three_prime_UTR 30.0%
GAAAAGCCAAACAAAAATCA+GGG - chr6_4:45619975-45619994 None:intergenic 30.0%
AGAAAAGCCAAACAAAAATC+AGG - chr6_4:45619976-45619995 None:intergenic 30.0%
GAAAGGTCTATATGTAAATG+AGG + chr6_4:45620017-45620036 Msa0989280:three_prime_UTR 30.0%
!! AGATATTTGCTACTTGCTAT+TGG + chr6_4:45618888-45618907 Msa0989280:intron 30.0%
!! AATGGAGTTGATATTGTTTC+TGG + chr6_4:45619042-45619061 Msa0989280:CDS 30.0%
!!! GCTCTTTTAATTTCTAAGTC+CGG + chr6_4:45619125-45619144 Msa0989280:intron 30.0%
! GAGAAAACCTTTTTCCAATT+AGG - chr6_4:45619216-45619235 None:intergenic 30.0%
AAGTACCAACATATACTTGA+TGG - chr6_4:45619282-45619301 None:intergenic 30.0%
ATATGTTGGTACTTCATACA+TGG + chr6_4:45619288-45619307 Msa0989280:intron 30.0%
GCCTAGTTTATTAAGTTCTT+CGG - chr6_4:45619408-45619427 None:intergenic 30.0%
! TTGTGGAACTCAAATTTTGT+TGG - chr6_4:45619452-45619471 None:intergenic 30.0%
! AACAAAATTTGAGTTCCACA+AGG + chr6_4:45619451-45619470 Msa0989280:CDS 30.0%
!! AGGAGCATTTCTAACTAATT+TGG + chr6_4:45619471-45619490 Msa0989280:exon 30.0%
TAGCTACAAAGAAGATGTAA+CGG + chr6_4:45619636-45619655 Msa0989280:three_prime_UTR 30.0%
AAGAAAGATAAAAGGAGGTA+AGG + chr6_4:45619779-45619798 Msa0989280:three_prime_UTR 30.0%
AGAAAGATAAAAGGAGGTAA+GGG + chr6_4:45619780-45619799 Msa0989280:three_prime_UTR 30.0%
GAAAAGCCAAACAAAAATCA+GGG - chr6_4:45619975-45619994 None:intergenic 30.0%
AGAAAAGCCAAACAAAAATC+AGG - chr6_4:45619976-45619995 None:intergenic 30.0%
GAAAGGTCTATATGTAAATG+AGG + chr6_4:45620017-45620036 Msa0989280:three_prime_UTR 30.0%
ATCGTCAATTTATGGACTTC+AGG + chr6_4:45618920-45618939 Msa0989280:intron 35.0%
TCGTCAATTTATGGACTTCA+GGG + chr6_4:45618921-45618940 Msa0989280:intron 35.0%
! ACTATGGTGATTGGTGATAA+GGG - chr6_4:45619000-45619019 None:intergenic 35.0%
! AACTATGGTGATTGGTGATA+AGG - chr6_4:45619001-45619020 None:intergenic 35.0%
ATGGTTAAGTGCTTGAACTA+TGG - chr6_4:45619016-45619035 None:intergenic 35.0%
!!! TATCAACTCCATTTTTGGCA+TGG - chr6_4:45619035-45619054 None:intergenic 35.0%
!! TTGATATTGTTTCTGGAACC+AGG + chr6_4:45619049-45619068 Msa0989280:CDS 35.0%
!! TGATATTGTTTCTGGAACCA+GGG + chr6_4:45619050-45619069 Msa0989280:CDS 35.0%
CAAAAATAGAATGCTTACCC+TGG - chr6_4:45619070-45619089 None:intergenic 35.0%
AGTCTCCATCAAGTATATGT+TGG + chr6_4:45619274-45619293 Msa0989280:intron 35.0%
GTACTTCATACATGGTAGTA+TGG + chr6_4:45619296-45619315 Msa0989280:intron 35.0%
TTGCTGTCTTAATTCGTATC+AGG + chr6_4:45619353-45619372 Msa0989280:intron 35.0%
!!! TGTATTTTATTTTGGTGCGC+TGG + chr6_4:45619376-45619395 Msa0989280:CDS 35.0%
GCCGAAGAACTTAATAAACT+AGG + chr6_4:45619404-45619423 Msa0989280:CDS 35.0%
TTAGTTAGAAATGCTCCTTG+TGG - chr6_4:45619469-45619488 None:intergenic 35.0%
!! CCACATCAATTTGCTATAAC+GGG - chr6_4:45619509-45619528 None:intergenic 35.0%
!! GCCACATCAATTTGCTATAA+CGG - chr6_4:45619510-45619529 None:intergenic 35.0%
TAGATCTGCAGTGAATTGAA+AGG + chr6_4:45619735-45619754 Msa0989280:three_prime_UTR 35.0%
AGATCTGCAGTGAATTGAAA+GGG + chr6_4:45619736-45619755 Msa0989280:three_prime_UTR 35.0%
GAAAGATAAAAGGAGGTAAG+GGG + chr6_4:45619781-45619800 Msa0989280:three_prime_UTR 35.0%
TAGAAACATAGGGCTGAAAA+TGG + chr6_4:45619873-45619892 Msa0989280:three_prime_UTR 35.0%
!!! TTTGTTTGGCTTTTCTACTG+AGG + chr6_4:45619980-45619999 Msa0989280:three_prime_UTR 35.0%
ATCGTCAATTTATGGACTTC+AGG + chr6_4:45618920-45618939 Msa0989280:intron 35.0%
TCGTCAATTTATGGACTTCA+GGG + chr6_4:45618921-45618940 Msa0989280:intron 35.0%
! ACTATGGTGATTGGTGATAA+GGG - chr6_4:45619000-45619019 None:intergenic 35.0%
! AACTATGGTGATTGGTGATA+AGG - chr6_4:45619001-45619020 None:intergenic 35.0%
ATGGTTAAGTGCTTGAACTA+TGG - chr6_4:45619016-45619035 None:intergenic 35.0%
!!! TATCAACTCCATTTTTGGCA+TGG - chr6_4:45619035-45619054 None:intergenic 35.0%
!! TTGATATTGTTTCTGGAACC+AGG + chr6_4:45619049-45619068 Msa0989280:CDS 35.0%
!! TGATATTGTTTCTGGAACCA+GGG + chr6_4:45619050-45619069 Msa0989280:CDS 35.0%
CAAAAATAGAATGCTTACCC+TGG - chr6_4:45619070-45619089 None:intergenic 35.0%
AGTCTCCATCAAGTATATGT+TGG + chr6_4:45619274-45619293 Msa0989280:intron 35.0%
GTACTTCATACATGGTAGTA+TGG + chr6_4:45619296-45619315 Msa0989280:intron 35.0%
TTGCTGTCTTAATTCGTATC+AGG + chr6_4:45619353-45619372 Msa0989280:intron 35.0%
!!! TGTATTTTATTTTGGTGCGC+TGG + chr6_4:45619376-45619395 Msa0989280:CDS 35.0%
GCCGAAGAACTTAATAAACT+AGG + chr6_4:45619404-45619423 Msa0989280:CDS 35.0%
TTAGTTAGAAATGCTCCTTG+TGG - chr6_4:45619469-45619488 None:intergenic 35.0%
!! CCACATCAATTTGCTATAAC+GGG - chr6_4:45619509-45619528 None:intergenic 35.0%
!! GCCACATCAATTTGCTATAA+CGG - chr6_4:45619510-45619529 None:intergenic 35.0%
TAGATCTGCAGTGAATTGAA+AGG + chr6_4:45619735-45619754 Msa0989280:three_prime_UTR 35.0%
AGATCTGCAGTGAATTGAAA+GGG + chr6_4:45619736-45619755 Msa0989280:three_prime_UTR 35.0%
GAAAGATAAAAGGAGGTAAG+GGG + chr6_4:45619781-45619800 Msa0989280:three_prime_UTR 35.0%
TAGAAACATAGGGCTGAAAA+TGG + chr6_4:45619873-45619892 Msa0989280:three_prime_UTR 35.0%
!!! TTTGTTTGGCTTTTCTACTG+AGG + chr6_4:45619980-45619999 Msa0989280:three_prime_UTR 35.0%
TATAGGGTTACAAGAGAGCA+AGG + chr6_4:45618774-45618793 Msa0989280:five_prime_UTR 40.0%
!!! GGCTCTTTTCATTGGTTCAA+AGG + chr6_4:45618795-45618814 Msa0989280:five_prime_UTR 40.0%
AAGTAGCTGAGCTTGATCAA+AGG + chr6_4:45618829-45618848 Msa0989280:five_prime_UTR 40.0%
AGTAGCTGAGCTTGATCAAA+GGG + chr6_4:45618830-45618849 Msa0989280:five_prime_UTR 40.0%
! AGCTTGATCAAAGGGTACAT+TGG + chr6_4:45618838-45618857 Msa0989280:intron 40.0%
AACTACTTAACTAGCGAGGA+AGG + chr6_4:45618960-45618979 Msa0989280:five_prime_UTR 40.0%
AGTGCTTGAACTATGGTGAT+TGG - chr6_4:45619009-45619028 None:intergenic 40.0%
GCACTTAACCATGCCAAAAA+TGG + chr6_4:45619024-45619043 Msa0989280:CDS 40.0%
! AGGTACAGACACATTTTGCT+AGG + chr6_4:45619190-45619209 Msa0989280:intron 40.0%
! CACATTTTGCTAGGCCTAAT+TGG + chr6_4:45619199-45619218 Msa0989280:CDS 40.0%
TGCTAGGCCTAATTGGAAAA+AGG + chr6_4:45619206-45619225 Msa0989280:CDS 40.0%
ATGTGTTCCAAGCACTATAG+TGG + chr6_4:45619240-45619259 Msa0989280:CDS 40.0%
TATTCGTCCACTATAGTGCT+TGG - chr6_4:45619250-45619269 None:intergenic 40.0%
!! AAGCACTATAGTGGACGAAT+AGG + chr6_4:45619249-45619268 Msa0989280:CDS 40.0%
ACAGCAATAATGTCAGAGCA+AGG - chr6_4:45619340-45619359 None:intergenic 40.0%
!!! TTATTTTGGTGCGCTGGTTT+TGG + chr6_4:45619382-45619401 Msa0989280:CDS 40.0%
! AGGCTTCGAGTTCAATGAAA+AGG + chr6_4:45619424-45619443 Msa0989280:CDS 40.0%
! GGCTTCGAGTTCAATGAAAA+GGG + chr6_4:45619425-45619444 Msa0989280:CDS 40.0%
CCCGTTATAGCAAATTGATG+TGG + chr6_4:45619506-45619525 Msa0989280:three_prime_UTR 40.0%
TATCAGACAGCAAACATGCT+TGG + chr6_4:45619597-45619616 Msa0989280:three_prime_UTR 40.0%
AATTGAAAGGGACAGTTCTG+AGG + chr6_4:45619748-45619767 Msa0989280:three_prime_UTR 40.0%
! ACCGAACCCTGATTTTTGTT+TGG + chr6_4:45619966-45619985 Msa0989280:three_prime_UTR 40.0%
GCCAAACAAAAATCAGGGTT+CGG - chr6_4:45619970-45619989 None:intergenic 40.0%
CATATAGACCTTTCCTCTGA+GGG - chr6_4:45620011-45620030 None:intergenic 40.0%
ACATATAGACCTTTCCTCTG+AGG - chr6_4:45620012-45620031 None:intergenic 40.0%
TATAGGGTTACAAGAGAGCA+AGG + chr6_4:45618774-45618793 Msa0989280:five_prime_UTR 40.0%
!!! GGCTCTTTTCATTGGTTCAA+AGG + chr6_4:45618795-45618814 Msa0989280:five_prime_UTR 40.0%
AAGTAGCTGAGCTTGATCAA+AGG + chr6_4:45618829-45618848 Msa0989280:five_prime_UTR 40.0%
AGTAGCTGAGCTTGATCAAA+GGG + chr6_4:45618830-45618849 Msa0989280:five_prime_UTR 40.0%
! AGCTTGATCAAAGGGTACAT+TGG + chr6_4:45618838-45618857 Msa0989280:intron 40.0%
AACTACTTAACTAGCGAGGA+AGG + chr6_4:45618960-45618979 Msa0989280:five_prime_UTR 40.0%
AGTGCTTGAACTATGGTGAT+TGG - chr6_4:45619009-45619028 None:intergenic 40.0%
GCACTTAACCATGCCAAAAA+TGG + chr6_4:45619024-45619043 Msa0989280:CDS 40.0%
! AGGTACAGACACATTTTGCT+AGG + chr6_4:45619190-45619209 Msa0989280:intron 40.0%
! CACATTTTGCTAGGCCTAAT+TGG + chr6_4:45619199-45619218 Msa0989280:CDS 40.0%
TGCTAGGCCTAATTGGAAAA+AGG + chr6_4:45619206-45619225 Msa0989280:CDS 40.0%
ATGTGTTCCAAGCACTATAG+TGG + chr6_4:45619240-45619259 Msa0989280:CDS 40.0%
TATTCGTCCACTATAGTGCT+TGG - chr6_4:45619250-45619269 None:intergenic 40.0%
!! AAGCACTATAGTGGACGAAT+AGG + chr6_4:45619249-45619268 Msa0989280:CDS 40.0%
ACAGCAATAATGTCAGAGCA+AGG - chr6_4:45619340-45619359 None:intergenic 40.0%
!!! TTATTTTGGTGCGCTGGTTT+TGG + chr6_4:45619382-45619401 Msa0989280:CDS 40.0%
! AGGCTTCGAGTTCAATGAAA+AGG + chr6_4:45619424-45619443 Msa0989280:CDS 40.0%
! GGCTTCGAGTTCAATGAAAA+GGG + chr6_4:45619425-45619444 Msa0989280:CDS 40.0%
CCCGTTATAGCAAATTGATG+TGG + chr6_4:45619506-45619525 Msa0989280:three_prime_UTR 40.0%
TATCAGACAGCAAACATGCT+TGG + chr6_4:45619597-45619616 Msa0989280:three_prime_UTR 40.0%
AATTGAAAGGGACAGTTCTG+AGG + chr6_4:45619748-45619767 Msa0989280:three_prime_UTR 40.0%
! ACCGAACCCTGATTTTTGTT+TGG + chr6_4:45619966-45619985 Msa0989280:three_prime_UTR 40.0%
GCCAAACAAAAATCAGGGTT+CGG - chr6_4:45619970-45619989 None:intergenic 40.0%
CATATAGACCTTTCCTCTGA+GGG - chr6_4:45620011-45620030 None:intergenic 40.0%
ACATATAGACCTTTCCTCTG+AGG - chr6_4:45620012-45620031 None:intergenic 40.0%
! GAGAGCAAGGCTCTTTTCAT+TGG + chr6_4:45618787-45618806 Msa0989280:five_prime_UTR 45.0%
GCACAACTACTTAACTAGCG+AGG + chr6_4:45618956-45618975 Msa0989280:five_prime_UTR 45.0%
TAAAAGGAGGTAAGGGGACA+AGG + chr6_4:45619787-45619806 Msa0989280:three_prime_UTR 45.0%
AAAAGGAGGTAAGGGGACAA+GGG + chr6_4:45619788-45619807 Msa0989280:three_prime_UTR 45.0%
ACGCTGGAATCGTCATGTAA+GGG + chr6_4:45619902-45619921 Msa0989280:three_prime_UTR 45.0%
TACTGAGGAATTTCCCTCAG+AGG + chr6_4:45619995-45620014 Msa0989280:three_prime_UTR 45.0%
AGGAATTTCCCTCAGAGGAA+AGG + chr6_4:45620000-45620019 Msa0989280:three_prime_UTR 45.0%
! AGCTCTAGTCGTAACCTTGA+TGG - chr6_4:45620068-45620087 None:intergenic 45.0%
! GAGAGCAAGGCTCTTTTCAT+TGG + chr6_4:45618787-45618806 Msa0989280:five_prime_UTR 45.0%
GCACAACTACTTAACTAGCG+AGG + chr6_4:45618956-45618975 Msa0989280:five_prime_UTR 45.0%
TAAAAGGAGGTAAGGGGACA+AGG + chr6_4:45619787-45619806 Msa0989280:three_prime_UTR 45.0%
AAAAGGAGGTAAGGGGACAA+GGG + chr6_4:45619788-45619807 Msa0989280:three_prime_UTR 45.0%
ACGCTGGAATCGTCATGTAA+GGG + chr6_4:45619902-45619921 Msa0989280:three_prime_UTR 45.0%
TACTGAGGAATTTCCCTCAG+AGG + chr6_4:45619995-45620014 Msa0989280:three_prime_UTR 45.0%
AGGAATTTCCCTCAGAGGAA+AGG + chr6_4:45620000-45620019 Msa0989280:three_prime_UTR 45.0%
! AGCTCTAGTCGTAACCTTGA+TGG - chr6_4:45620068-45620087 None:intergenic 45.0%
AGGTAAGGGGACAAGGGATT+TGG + chr6_4:45619794-45619813 Msa0989280:three_prime_UTR 50.0%
CTGAAAATGGTGACTCACGC+TGG + chr6_4:45619886-45619905 Msa0989280:three_prime_UTR 50.0%
CACGCTGGAATCGTCATGTA+AGG + chr6_4:45619901-45619920 Msa0989280:three_prime_UTR 50.0%
! ATCAGGGTTCGGTTGAGAGA+CGG - chr6_4:45619959-45619978 None:intergenic 50.0%
AGGTAAGGGGACAAGGGATT+TGG + chr6_4:45619794-45619813 Msa0989280:three_prime_UTR 50.0%
CTGAAAATGGTGACTCACGC+TGG + chr6_4:45619886-45619905 Msa0989280:three_prime_UTR 50.0%
CACGCTGGAATCGTCATGTA+AGG + chr6_4:45619901-45619920 Msa0989280:three_prime_UTR 50.0%
! ATCAGGGTTCGGTTGAGAGA+CGG - chr6_4:45619959-45619978 None:intergenic 50.0%
TCGGTTGAGAGACGGAGAGA+GGG - chr6_4:45619951-45619970 None:intergenic 55.0%
TTCGGTTGAGAGACGGAGAG+AGG - chr6_4:45619952-45619971 None:intergenic 55.0%
TCGGTTGAGAGACGGAGAGA+GGG - chr6_4:45619951-45619970 None:intergenic 55.0%
TTCGGTTGAGAGACGGAGAG+AGG - chr6_4:45619952-45619971 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 45618769 45620213 45618769 ID=Msa0989280;Name=Msa0989280
chr6_4 mRNA 45618769 45620213 45618769 ID=Msa0989280-mRNA-1;Parent=Msa0989280;Name=Msa0989280-mRNA-1;_AED=0.10;_eAED=0.10;_QI=126|1|1|1|0.66|0.5|4|729|91
chr6_4 exon 45618769 45618851 45618769 ID=Msa0989280-mRNA-1:exon:10278;Parent=Msa0989280-mRNA-1
chr6_4 exon 45618942 45619071 45618942 ID=Msa0989280-mRNA-1:exon:10279;Parent=Msa0989280-mRNA-1
chr6_4 exon 45619192 45619270 45619192 ID=Msa0989280-mRNA-1:exon:10280;Parent=Msa0989280-mRNA-1
chr6_4 exon 45619375 45620213 45619375 ID=Msa0989280-mRNA-1:exon:10281;Parent=Msa0989280-mRNA-1
chr6_4 five_prime_UTR 45618769 45618851 45618769 ID=Msa0989280-mRNA-1:five_prime_utr;Parent=Msa0989280-mRNA-1
chr6_4 five_prime_UTR 45618942 45618984 45618942 ID=Msa0989280-mRNA-1:five_prime_utr;Parent=Msa0989280-mRNA-1
chr6_4 CDS 45618985 45619071 45618985 ID=Msa0989280-mRNA-1:cds;Parent=Msa0989280-mRNA-1
chr6_4 CDS 45619192 45619270 45619192 ID=Msa0989280-mRNA-1:cds;Parent=Msa0989280-mRNA-1
chr6_4 CDS 45619375 45619484 45619375 ID=Msa0989280-mRNA-1:cds;Parent=Msa0989280-mRNA-1
chr6_4 three_prime_UTR 45619485 45620213 45619485 ID=Msa0989280-mRNA-1:three_prime_utr;Parent=Msa0989280-mRNA-1
Gene Sequence

>Msa0989280

ATGCTAGATCTGCCCTTATCACCAATCACCATAGTTCAAGCACTTAACCATGCCAAAAATGGAGTTGATATTGTTTCTGGAACCAGGGTACAGACACATTTTGCTAGGCCTAATTGGAAAAAGGTTTTCTCTAAAATGTGTTCCAAGCACTATAGTGGACGAATAGGTGTATTTTATTTTGGTGCGCTGGTTTTGGCCGAAGAACTTAATAAACTAGGCTTCGAGTTCAATGAAAAGGGTCCAACAAAATTTGAGTTCCACAAGGAGCATTTCTAA
Protein sequence

>Msa0989280

MLDLPLSPITIVQALNHAKNGVDIVSGTRVQTHFARPNWKKVFSKMCSKHYSGRIGVFYFGALVLAEELNKLGFEFNEKGPTKFEFHKEHF*