Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0993480 | XP_003619574.1 | 94.064 | 219 | 13 | 0 | 1 | 219 | 1 | 219 | 1.12e-129 | 374 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0993480 | sp|O82711|PIP22_PEA | 70.455 | 220 | 62 | 1 | 1 | 220 | 1 | 217 | 3.81e-109 | 315 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0993480 | G7KMU3 | 94.064 | 219 | 13 | 0 | 1 | 219 | 1 | 219 | 5.37e-130 | 374 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0188040 | Msa0993480 | 0.803323 | 3.681794e-49 | -8.615850e-47 |
| Msa0277780 | Msa0993480 | 0.811733 | 6.038626e-51 | -8.615850e-47 |
| Msa0920520 | Msa0993480 | 0.818039 | 2.412862e-52 | -8.615850e-47 |
| Msa0920530 | Msa0993480 | 0.823686 | 1.212115e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0993480 | MtrunA17_Chr6g0476871 | 94.064 | 219 | 13 | 0 | 1 | 219 | 1 | 219 | 1.03e-133 | 374 |
| Msa0993480 | MtrunA17_Chr6g0476831 | 69.406 | 219 | 63 | 2 | 1 | 219 | 1 | 215 | 1.54e-97 | 283 |
| Msa0993480 | MtrunA17_Chr6g0477741 | 52.804 | 214 | 95 | 3 | 1 | 214 | 1 | 208 | 1.87e-65 | 201 |
| Msa0993480 | MtrunA17_Chr6g0452491 | 51.442 | 208 | 95 | 3 | 1 | 208 | 1 | 202 | 3.36e-63 | 195 |
| Msa0993480 | MtrunA17_Chr6g0476791 | 52.404 | 208 | 91 | 6 | 7 | 210 | 10 | 213 | 7.75e-61 | 189 |
| Msa0993480 | MtrunA17_Chr6g0452521 | 51.196 | 209 | 95 | 4 | 1 | 208 | 33 | 235 | 1.34e-60 | 190 |
| Msa0993480 | MtrunA17_Chr6g0477751 | 50.000 | 216 | 100 | 4 | 1 | 214 | 1 | 210 | 4.99e-58 | 182 |
| Msa0993480 | MtrunA17_Chr1g0163381 | 48.458 | 227 | 105 | 8 | 1 | 219 | 1 | 223 | 2.56e-57 | 181 |
| Msa0993480 | MtrunA17_Chr6g0476921 | 46.635 | 208 | 84 | 9 | 22 | 220 | 22 | 211 | 2.37e-43 | 145 |
| Msa0993480 | MtrunA17_Chr6g0476951 | 47.273 | 220 | 95 | 11 | 1 | 216 | 1 | 203 | 1.83e-42 | 142 |
| Msa0993480 | MtrunA17_Chr6g0476961 | 47.727 | 220 | 93 | 12 | 1 | 216 | 1 | 202 | 5.62e-40 | 135 |
| Msa0993480 | MtrunA17_Chr6g0477281 | 50.588 | 170 | 78 | 5 | 7 | 173 | 6 | 172 | 6.04e-38 | 130 |
| Msa0993480 | MtrunA17_Chr3g0104591 | 47.568 | 185 | 81 | 7 | 32 | 209 | 29 | 204 | 3.29e-37 | 129 |
| Msa0993480 | MtrunA17_Chr6g0457121 | 64.356 | 101 | 32 | 2 | 1 | 101 | 26 | 122 | 1.20e-35 | 122 |
| Msa0993480 | MtrunA17_Chr6g0467621 | 39.631 | 217 | 109 | 11 | 1 | 207 | 1 | 205 | 1.98e-28 | 106 |
| Msa0993480 | MtrunA17_Chr6g0467611 | 40.000 | 215 | 111 | 9 | 1 | 207 | 1 | 205 | 1.31e-27 | 104 |
| Msa0993480 | MtrunA17_Chr6g0467761 | 41.000 | 200 | 101 | 8 | 1 | 193 | 1 | 190 | 2.23e-27 | 104 |
| Msa0993480 | MtrunA17_Chr6g0467771 | 36.967 | 211 | 121 | 8 | 1 | 207 | 1 | 203 | 2.16e-23 | 93.6 |
| Msa0993480 | MtrunA17_Chr6g0481911 | 34.783 | 207 | 120 | 9 | 10 | 208 | 4 | 203 | 2.48e-21 | 87.8 |
| Msa0993480 | MtrunA17_Chr6g0481941 | 34.884 | 215 | 114 | 10 | 5 | 208 | 6 | 205 | 1.27e-19 | 83.2 |
| Msa0993480 | MtrunA17_Chr6g0467781 | 33.526 | 173 | 97 | 8 | 55 | 220 | 13 | 174 | 4.73e-16 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0993480 | AT1G17860.1 | 32.663 | 199 | 121 | 7 | 11 | 208 | 9 | 195 | 3.79e-17 | 77.0 |
Find 48 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAGATGGATTTGGTTAT+AGG | 0.218156 | 6_4:-66035078 | Msa0993480:CDS |
| GAACAAGTATTGGACATAAA+TGG | 0.312320 | 6_4:-66035460 | Msa0993480:CDS |
| GAAAGATTCTCCTACTTGTT+TGG | 0.325215 | 6_4:-66035041 | Msa0993480:CDS |
| GAGAAACATGAAGATGGATT+TGG | 0.333013 | 6_4:-66035085 | Msa0993480:CDS |
| CTCCTACTTGTTTGGATAGT+GGG | 0.339355 | 6_4:-66035033 | Msa0993480:CDS |
| CCAAGGGGTCCTCGAATTGC+AGG | 0.355865 | 6_4:+66035400 | None:intergenic |
| TTTGATGATTCAGCGCAATT+AGG | 0.356654 | 6_4:+66035211 | None:intergenic |
| ATTGCGCTGAATCATCAAAA+TGG | 0.370243 | 6_4:-66035207 | Msa0993480:CDS |
| GATGCTGCTTCTTACGAAAC+TGG | 0.385642 | 6_4:-66034929 | Msa0993480:CDS |
| GATGGATTTGGTTATAGGCT+TGG | 0.395112 | 6_4:-66035073 | Msa0993480:CDS |
| ACTAGGAAAATCAAGTAATT+CGG | 0.402253 | 6_4:-66035365 | Msa0993480:CDS |
| TCTCCTACTTGTTTGGATAG+TGG | 0.405756 | 6_4:-66035034 | Msa0993480:CDS |
| TGAGAAAGCTAGTGAAAGAT+TGG | 0.414943 | 6_4:+66035495 | None:intergenic |
| TTTGTGAACTCGATCTCAAT+TGG | 0.421811 | 6_4:+66035238 | None:intergenic |
| ATTATACCAGGACTTATTCC+TGG | 0.426390 | 6_4:+66035274 | None:intergenic |
| GAATGCTAAAACTTGCTTCA+TGG | 0.431729 | 6_4:+66035549 | None:intergenic |
| TGATGTTGTTGAACAAGTAT+TGG | 0.456591 | 6_4:-66035470 | Msa0993480:CDS |
| AATATTGAGAAACATGAAGA+TGG | 0.464831 | 6_4:-66035091 | Msa0993480:CDS |
| CCTGCAATTCGAGGACCCCT+TGG | 0.473846 | 6_4:-66035400 | Msa0993480:CDS |
| GGTGGTCCTGAAAATTATCC+TGG | 0.475229 | 6_4:-66035139 | Msa0993480:CDS |
| GCAATTCGAGGACCCCTTGG+TGG | 0.502881 | 6_4:-66035397 | Msa0993480:CDS |
| CCAGTGAAATTCAGTATACC+AGG | 0.505220 | 6_4:-66035292 | Msa0993480:CDS |
| AAAGCTTGTGTTGGTATTGG+TGG | 0.506283 | 6_4:-66035157 | Msa0993480:CDS |
| TACTTGTTTGGATAGTGGGA+GGG | 0.509435 | 6_4:-66035029 | Msa0993480:CDS |
| AACAAAGAGGAGGAAGGAAA+GGG | 0.514918 | 6_4:+66035525 | None:intergenic |
| CCTGGTATACTGAATTTCAC+TGG | 0.516748 | 6_4:+66035292 | None:intergenic |
| CATGAAGAGGTGGAGGATGA+AGG | 0.524538 | 6_4:-66035004 | Msa0993480:CDS |
| AGTCCTGGTATAATCTTCAC+TGG | 0.527399 | 6_4:-66035265 | Msa0993480:CDS |
| ATTCGAGGACCCCTTGGTGG+AGG | 0.529072 | 6_4:-66035394 | Msa0993480:CDS |
| CTACTTGTTTGGATAGTGGG+AGG | 0.534160 | 6_4:-66035030 | Msa0993480:CDS |
| TTTACATTTGACTCATCAAG+TGG | 0.535143 | 6_4:-66034972 | Msa0993480:CDS |
| AAACAAAGAGGAGGAAGGAA+AGG | 0.540329 | 6_4:+66035524 | None:intergenic |
| GATTACAATGAAGTTATCAA+TGG | 0.556101 | 6_4:-66035319 | Msa0993480:CDS |
| GAGGGCTCATGAAGAGGTGG+AGG | 0.562944 | 6_4:-66035011 | Msa0993480:CDS |
| AGTGAAAACAAAGAGGAGGA+AGG | 0.568419 | 6_4:+66035519 | None:intergenic |
| TAGTCTTAATCCTCCACCAA+GGG | 0.575545 | 6_4:+66035384 | None:intergenic |
| CTTGGTGGAGGATTAAGACT+AGG | 0.578734 | 6_4:-66035382 | Msa0993480:CDS |
| CCACTCAATATTCTAAAACC+AGG | 0.581410 | 6_4:+66035121 | None:intergenic |
| TAGTGGGAGGGCTCATGAAG+AGG | 0.585537 | 6_4:-66035017 | Msa0993480:CDS |
| AGTATACCAGGAATAAGTCC+TGG | 0.585812 | 6_4:-66035280 | Msa0993480:CDS |
| TGGTAGTGAAAACAAAGAGG+AGG | 0.608022 | 6_4:+66035515 | None:intergenic |
| GAAGAGGTGGAGGATGAAGG+TGG | 0.620322 | 6_4:-66035001 | Msa0993480:CDS |
| TGGGAGGGCTCATGAAGAGG+TGG | 0.636422 | 6_4:-66035014 | Msa0993480:CDS |
| GATTGGTAGTGAAAACAAAG+AGG | 0.642246 | 6_4:+66035512 | None:intergenic |
| CTCCCACTATCCAAACAAGT+AGG | 0.657077 | 6_4:+66035031 | None:intergenic |
| CTAGTCTTAATCCTCCACCA+AGG | 0.657509 | 6_4:+66035383 | None:intergenic |
| GTACCAGTGAAGATTATACC+AGG | 0.672259 | 6_4:+66035262 | None:intergenic |
| AGTCTTAATCCTCCACCAAG+GGG | 0.703379 | 6_4:+66035385 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTAGGAAAATCAAGTAATT+CGG | - | chr6_4:66035089-66035108 | Msa0993480:CDS | 25.0% |
| ! | GATTACAATGAAGTTATCAA+TGG | - | chr6_4:66035135-66035154 | Msa0993480:CDS | 25.0% |
| ! | AATATTGAGAAACATGAAGA+TGG | - | chr6_4:66035363-66035382 | Msa0993480:CDS | 25.0% |
| TGATGTTGTTGAACAAGTAT+TGG | - | chr6_4:66034984-66035003 | Msa0993480:CDS | 30.0% | |
| !! | GAACAAGTATTGGACATAAA+TGG | - | chr6_4:66034994-66035013 | Msa0993480:CDS | 30.0% |
| ! | TATTTTCCACCTGGAAAAAT+GGG | + | chr6_4:66035024-66035043 | None:intergenic | 30.0% |
| ! | AAAATGTAGTATTTTCCACC+TGG | + | chr6_4:66035033-66035052 | None:intergenic | 30.0% |
| GTTATTCAAAAAGCTTGTGT+TGG | - | chr6_4:66035288-66035307 | Msa0993480:CDS | 30.0% | |
| ! | CTAAAACCAGGATAATTTTC+AGG | + | chr6_4:66035324-66035343 | None:intergenic | 30.0% |
| ! | ATGAAGATGGATTTGGTTAT+AGG | - | chr6_4:66035376-66035395 | Msa0993480:CDS | 30.0% |
| TTTACATTTGACTCATCAAG+TGG | - | chr6_4:66035482-66035501 | Msa0993480:CDS | 30.0% | |
| !! | GATTGGTAGTGAAAACAAAG+AGG | + | chr6_4:66034945-66034964 | None:intergenic | 35.0% |
| TGAGAAAGCTAGTGAAAGAT+TGG | + | chr6_4:66034962-66034981 | None:intergenic | 35.0% | |
| ! | ATTTTCCACCTGGAAAAATG+GGG | + | chr6_4:66035023-66035042 | None:intergenic | 35.0% |
| ! | GTATTTTCCACCTGGAAAAA+TGG | + | chr6_4:66035025-66035044 | None:intergenic | 35.0% |
| ATTATACCAGGACTTATTCC+TGG | + | chr6_4:66035183-66035202 | None:intergenic | 35.0% | |
| TTTGTGAACTCGATCTCAAT+TGG | + | chr6_4:66035219-66035238 | None:intergenic | 35.0% | |
| ! | TTTGATGATTCAGCGCAATT+AGG | + | chr6_4:66035246-66035265 | None:intergenic | 35.0% |
| ATTGCGCTGAATCATCAAAA+TGG | - | chr6_4:66035247-66035266 | Msa0993480:CDS | 35.0% | |
| ! | CAAAAAGCTTGTGTTGGTAT+TGG | - | chr6_4:66035294-66035313 | Msa0993480:CDS | 35.0% |
| !! | CCACTCAATATTCTAAAACC+AGG | + | chr6_4:66035336-66035355 | None:intergenic | 35.0% |
| !!! | CCTGGTTTTAGAATATTGAG+TGG | - | chr6_4:66035333-66035352 | Msa0993480:CDS | 35.0% |
| ! | GAGAAACATGAAGATGGATT+TGG | - | chr6_4:66035369-66035388 | Msa0993480:CDS | 35.0% |
| GAAAGATTCTCCTACTTGTT+TGG | - | chr6_4:66035413-66035432 | Msa0993480:CDS | 35.0% | |
| AACAAAGAGGAGGAAGGAAA+GGG | + | chr6_4:66034932-66034951 | None:intergenic | 40.0% | |
| AAACAAAGAGGAGGAAGGAA+AGG | + | chr6_4:66034933-66034952 | None:intergenic | 40.0% | |
| AGTGAAAACAAAGAGGAGGA+AGG | + | chr6_4:66034938-66034957 | None:intergenic | 40.0% | |
| ! | TGGTAGTGAAAACAAAGAGG+AGG | + | chr6_4:66034942-66034961 | None:intergenic | 40.0% |
| !! | AATGGTAACCCCATTTTTCC+AGG | - | chr6_4:66035012-66035031 | Msa0993480:CDS | 40.0% |
| ! | TACATTTTGCCTGCAATTCG+AGG | - | chr6_4:66035045-66035064 | Msa0993480:CDS | 40.0% |
| ! | TAGTCTTAATCCTCCACCAA+GGG | + | chr6_4:66035073-66035092 | None:intergenic | 40.0% |
| CCTGGTATACTGAATTTCAC+TGG | + | chr6_4:66035165-66035184 | None:intergenic | 40.0% | |
| CCAGTGAAATTCAGTATACC+AGG | - | chr6_4:66035162-66035181 | Msa0993480:CDS | 40.0% | |
| AGTATACCAGGAATAAGTCC+TGG | - | chr6_4:66035174-66035193 | Msa0993480:CDS | 40.0% | |
| AGTCCTGGTATAATCTTCAC+TGG | - | chr6_4:66035189-66035208 | Msa0993480:CDS | 40.0% | |
| GTACCAGTGAAGATTATACC+AGG | + | chr6_4:66035195-66035214 | None:intergenic | 40.0% | |
| ! | AAAGCTTGTGTTGGTATTGG+TGG | - | chr6_4:66035297-66035316 | Msa0993480:CDS | 40.0% |
| ! | GATGGATTTGGTTATAGGCT+TGG | - | chr6_4:66035381-66035400 | Msa0993480:CDS | 40.0% |
| TCTCCTACTTGTTTGGATAG+TGG | - | chr6_4:66035420-66035439 | Msa0993480:CDS | 40.0% | |
| CTCCTACTTGTTTGGATAGT+GGG | - | chr6_4:66035421-66035440 | Msa0993480:CDS | 40.0% | |
| !! | TACTTGTTTGGATAGTGGGA+GGG | - | chr6_4:66035425-66035444 | Msa0993480:CDS | 40.0% |
| AGTCTTAATCCTCCACCAAG+GGG | + | chr6_4:66035072-66035091 | None:intergenic | 45.0% | |
| !! | CTAGTCTTAATCCTCCACCA+AGG | + | chr6_4:66035074-66035093 | None:intergenic | 45.0% |
| CTTGGTGGAGGATTAAGACT+AGG | - | chr6_4:66035072-66035091 | Msa0993480:CDS | 45.0% | |
| GGTGGTCCTGAAAATTATCC+TGG | - | chr6_4:66035315-66035334 | Msa0993480:CDS | 45.0% | |
| CTCCCACTATCCAAACAAGT+AGG | + | chr6_4:66035426-66035445 | None:intergenic | 45.0% | |
| !! | CTACTTGTTTGGATAGTGGG+AGG | - | chr6_4:66035424-66035443 | Msa0993480:CDS | 45.0% |
| ! | GACTCATCAAGTGGCTTTTG+CGG | - | chr6_4:66035491-66035510 | Msa0993480:CDS | 45.0% |
| ! | GATGCTGCTTCTTACGAAAC+TGG | - | chr6_4:66035525-66035544 | Msa0993480:CDS | 45.0% |
| !! | GGTAACCCCATTTTTCCAGG+TGG | - | chr6_4:66035015-66035034 | Msa0993480:CDS | 50.0% |
| CATGAAGAGGTGGAGGATGA+AGG | - | chr6_4:66035450-66035469 | Msa0993480:CDS | 50.0% | |
| TAGTGGGAGGGCTCATGAAG+AGG | - | chr6_4:66035437-66035456 | Msa0993480:CDS | 55.0% | |
| GAAGAGGTGGAGGATGAAGG+TGG | - | chr6_4:66035453-66035472 | Msa0993480:CDS | 55.0% | |
| CCAAGGGGTCCTCGAATTGC+AGG | + | chr6_4:66035057-66035076 | None:intergenic | 60.0% | |
| CCTGCAATTCGAGGACCCCT+TGG | - | chr6_4:66035054-66035073 | Msa0993480:CDS | 60.0% | |
| GCAATTCGAGGACCCCTTGG+TGG | - | chr6_4:66035057-66035076 | Msa0993480:CDS | 60.0% | |
| ATTCGAGGACCCCTTGGTGG+AGG | - | chr6_4:66035060-66035079 | Msa0993480:CDS | 60.0% | |
| TGGGAGGGCTCATGAAGAGG+TGG | - | chr6_4:66035440-66035459 | Msa0993480:CDS | 60.0% | |
| GAGGGCTCATGAAGAGGTGG+AGG | - | chr6_4:66035443-66035462 | Msa0993480:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 66034907 | 66035569 | 66034907 | ID=Msa0993480;Name=Msa0993480 |
| chr6_4 | mRNA | 66034907 | 66035569 | 66034907 | ID=Msa0993480-mRNA-1;Parent=Msa0993480;Name=Msa0993480-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|220 |
| chr6_4 | exon | 66034907 | 66035569 | 66034907 | ID=Msa0993480-mRNA-1:exon:12341;Parent=Msa0993480-mRNA-1 |
| chr6_4 | CDS | 66034907 | 66035569 | 66034907 | ID=Msa0993480-mRNA-1:cds;Parent=Msa0993480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
