AlfalfaGEDB | Msa0995710

M. sativa cultivar zhongmu-4 / Msa0995710

KEGG: -
GO: -
PFAM: PF04937.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0995710	XP_004493998.1	60.000	695	194	7	16	635	47	732	0.0	837

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0995710	A0A1S2XST3	60.000	695	194	7	16	635	47	732	0.0	837

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0063880	Msa0995710	0.801119	1.046312e-48	-8.615850e-47
Msa0859400	Msa0995710	0.911516	5.565327e-83	-8.615850e-47
Msa0931520	Msa0995710	0.801275	9.725068e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0995710	MtrunA17_Chr2g0302451	29.764	635	346	9	15	549	157	791	5.05e-86	288
Msa0995710	MtrunA17_Chr3g0112101	32.573	482	257	5	137	551	204	684	9.31e-77	261
Msa0995710	MtrunA17_Chr6g0454051	31.570	567	301	10	62	550	61	618	2.13e-75	255
Msa0995710	MtrunA17_Chr3g0126261	29.730	555	301	12	137	611	23	568	2.34e-72	244
Msa0995710	MtrunA17_Chr6g0454061	33.400	500	254	7	126	550	68	563	2.35e-72	246
Msa0995710	MtrunA17_Chr6g0455641	33.546	471	243	7	127	528	68	537	1.41e-70	241
Msa0995710	MtrunA17_Chr6g0454041	29.885	609	320	10	14	550	92	665	1.52e-68	238
Msa0995710	MtrunA17_Chr6g0476101	43.200	250	119	3	248	475	15	263	8.56e-64	213
Msa0995710	MtrunA17_Chr4g0010371	52.632	57	27	0	467	523	44	100	8.19e-14	67.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0995710	AT5G33406.1	39.788	377	154	5	337	641	1	376	1.91e-85	277
Msa0995710	AT4G15020.2	30.000	670	363	13	18	583	45	712	1.65e-84	282
Msa0995710	AT4G15020.1	30.000	670	363	13	18	583	45	712	1.65e-84	282
Msa0995710	AT3G17450.2	28.302	636	354	14	15	550	157	790	3.78e-81	275
Msa0995710	AT3G17450.1	28.302	636	354	14	15	550	157	790	3.78e-81	275
Msa0995710	AT3G22220.3	31.429	525	284	9	121	573	177	697	2.89e-76	259
Msa0995710	AT3G22220.4	31.429	525	284	9	121	573	177	697	2.89e-76	259
Msa0995710	AT3G22220.2	31.429	525	284	9	121	573	177	697	2.89e-76	259
Msa0995710	AT3G22220.1	31.429	525	284	9	121	573	177	697	2.89e-76	259
Msa0995710	AT1G79740.1	29.468	526	276	13	137	587	112	617	1.59e-67	233

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Find 135 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTTCAAAGAAATGT+TGG	0.489376	6_4:-72415010	Msa0995710:CDS
AAAGAAATGTTGGAAGCTGT+TGG	0.449680	6_4:-72415000	Msa0995710:CDS
AAAGATCCCAAATTACTGAG+AGG	0.672180	6_4:-72413979	Msa0995710:intron
AACAATGCCTTAACCTCTGC+TGG	0.488665	6_4:+72415348	None:intergenic
AACAGGGGAGATGGGAGAGA+TGG	0.413136	6_4:-72413365	Msa0995710:CDS
AACCAAACTCAGCACTTTGA+TGG	0.386442	6_4:+72413679	None:intergenic
AACTCCGGATTTGACACTAA+TGG	0.490085	6_4:-72413702	Msa0995710:CDS
AAGAAGAAATTGAAGAAATA+AGG	0.315176	6_4:-72415245	Msa0995710:CDS
AAGACACCAATTGGGAATTT+CGG	0.269397	6_4:-72415397	Msa0995710:CDS
AAGATATTGGTAAGATTCCT+AGG	0.417549	6_4:-72414552	Msa0995710:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGCTTGGCAATTTAATCAT+TGG	-	chr6_4:72413214-72413233	Msa0995710:CDS	30.0%
TTGGCAATTTAATCATTGGA+AGG	-	chr6_4:72413218-72413237	Msa0995710:CDS	30.0%
TTAGTGTGATTTCTGTGATA+AGG	-	chr6_4:72413265-72413284	Msa0995710:CDS	30.0%
TCCATAAACATTACATCCAA+GGG	+	chr6_4:72413557-72413576	None:intergenic	30.0%
AATATTTGTTTGCCTAGTAC+TGG	+	chr6_4:72413591-72413610	None:intergenic	30.0%
GAATCGAGCAATAAATTGTA+TGG	+	chr6_4:72413645-72413664	None:intergenic	30.0%
CTAATTTCATAATAGCTTGG+TGG	+	chr6_4:72413761-72413780	None:intergenic	30.0%
CACCAAGCTATTATGAAATT+AGG	-	chr6_4:72413759-72413778	Msa0995710:intron	30.0%
CCAAGCTATTATGAAATTAG+GGG	-	chr6_4:72413761-72413780	Msa0995710:intron	30.0%
AAGATATTGGTAAGATTCCT+AGG	-	chr6_4:72414161-72414180	Msa0995710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	72413123	72415612	72413123	ID=Msa0995710;Name=Msa0995710
chr6_4	mRNA	72413123	72415612	72413123	ID=Msa0995710-mRNA-1;Parent=Msa0995710;Name=Msa0995710-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|0\|0\|0.2\|1\|1\|5\|0\|659
chr6_4	exon	72415571	72415612	72415571	ID=Msa0995710-mRNA-1:exon:13530;Parent=Msa0995710-mRNA-1
chr6_4	exon	72414191	72415448	72414191	ID=Msa0995710-mRNA-1:exon:13529;Parent=Msa0995710-mRNA-1
chr6_4	exon	72413980	72414123	72413980	ID=Msa0995710-mRNA-1:exon:13528;Parent=Msa0995710-mRNA-1
chr6_4	exon	72413634	72413752	72413634	ID=Msa0995710-mRNA-1:exon:13527;Parent=Msa0995710-mRNA-1
chr6_4	exon	72413123	72413539	72413123	ID=Msa0995710-mRNA-1:exon:13526;Parent=Msa0995710-mRNA-1
chr6_4	CDS	72415571	72415612	72415571	ID=Msa0995710-mRNA-1:cds;Parent=Msa0995710-mRNA-1
chr6_4	CDS	72414191	72415448	72414191	ID=Msa0995710-mRNA-1:cds;Parent=Msa0995710-mRNA-1
chr6_4	CDS	72413980	72414123	72413980	ID=Msa0995710-mRNA-1:cds;Parent=Msa0995710-mRNA-1
chr6_4	CDS	72413634	72413752	72413634	ID=Msa0995710-mRNA-1:cds;Parent=Msa0995710-mRNA-1
chr6_4	CDS	72413123	72413539	72413123	ID=Msa0995710-mRNA-1:cds;Parent=Msa0995710-mRNA-1

Gene Sequence

>Msa0995710

ATGGCAGCATCAGCTTCTGGTGTTCAATCAAACTCTGTGTCTGTCAGCAGTGGAGGAATTAGTAGAGCAAGAAGACACCAATTGGGAATTTCGGGTGATGTAAAAGCATGTCGTAAAACACCAGCAGAGGTTAAGGCATTGTTGCAAGCTGATTTGGATGAGAAAAAAAAAAGGAAATCAGAAGGTATTGCAGAGGTCCAAGCTCTACTAGATGATGATGTTCAAGAAGAAATTGAAGAAATAAGGAGAATTCGAACTGGAAAGAGGCCATCTGATTCATCTTTGTCTGCTCCCAAAGCCAAGAAAGGTCCCTTGGATGTAATGTTTATGGAGAAACAAACTACCAGTACTAGGCAAACAAATATTAAAGATGCTGACAAAGAAGCAAGGGCAAGAACCATACAATTTATTGCTCGATTCTTTTATACAAATGGAATTGCTTTTAATGTTTCACGGTCAAAACACTTCAAAGAAATGTTGGAAGCTGTTGGAGCTTATGGTCATCATTTGAAACCACCAAGCTATTATGAAATTAGGGGACCACTACTTCAAATAGAGTTGGAAGAAACTAAGAAGATGTTGGAGCCTCATTTGGCCGCAAGGGTAAACTATGGATGCTCGATTATGTCCGATGGTTGGACAGACCGAAAAGGTAGAACCTTGATTAATTTTTTGGTGAATTCTACTGCTGGAACAATGTTTGTCAAAAGTATTGATGCTTCTGAAGAGTCAAAGACTGGAGAAATGGTGTATAAATTGATGAGTGATTTTGTTGATGAGATGGGTGAGCAAAATGTGGTGCAATTGATTACTGACAATGGAAGCAACTATGTTAATGCTGGCAAAAAGTTAATGCAGAACAAACCTCACATCTTTTGGACTCCATGTGCTGCACACTGCATAGACCTCATGTTGGAAGATATTGGTAAGATTCCTAGGATTAAAAAGGCTATACAAAAGGGGTGTTCTCTTGTAGGCTACATTTCTAACCACACATTTGCTTTGAGCCTAATGAGAAAGAAAACCAACAATGTTGAGTTGGTTAAACATGGAGTCACAAGGTTTGCTACCACATTTCTTACTTTGCAAAGGTTACACAAAATAAAATCCAAGCTTAGAGAGATGTTTTTATGTGAAGAATGGTTGAGTTCCAAGTGCAATAAGGAAGCTAAAGGTAGGCAGGCAGCAACATGTGCACTTCAGCCATCATTTTGGAATGATGTACTCCACACTCTTAAGTCCATGGCACCACTTGTTGACGTGCTAAGAATGGTTGATAATGAGAAAAAACCTGCAATGGGTCAGTATAAAGATGTGTTCAAAATCATTGATAAAAGATGGGAGTGCCAACTTCATCATCCATTGCATGCTGCTGGTTACTACCTCAATCCTAAATACTTTTATAGTAAACCAGATATTGAGAAAGATCCCAAATTACTGAGAGCTGAGTGGTGGATGAATTATGGAGCAGAAACTCCGGATTTGACACTAATGGCCATCAAAGTGCTGAGTTTGGTTTGTAGTGCTTCAGGATGTGAGCGCAACTGGTCTACTTTTGAGCATATTCACTCCAAGAAGAGAAGTAAACTTGAGCATCAAAAGTTGCAAGAGTTGGTTTTCATTAAGTATAACCAAGCTTTGAAGGACCGATATGATTGTCTTGACAAGATTGACCCCGTGATGTTGTATGATTTTAATTATCATACTGAATGGTTAACAGGGGAGATGGGAGAGATGGGAGAGGATGGTGAACCTGTTGAAGAATTTGTTCATGAAAATGATGATCTAACTTGGGATCAAGTTGCCAATGCAAGTGGGGTTAATGAGCCTATTGTGAACACAAGGGCTAGTATGAGGGCAGAACGAGCAAAGGAACTTGCTGCAGCTGCAAGTTCAAATAGAGCATCAACATCAATTAGACAAGAAGTATTTGTTGAAGATGTTGTAGGAATTGAAGAAGAAGATGATGATGATAATTGA

Protein sequence

>Msa0995710

MAASASGVQSNSVSVSSGGISRARRHQLGISGDVKACRKTPAEVKALLQADLDEKKKRKSEGIAEVQALLDDDVQEEIEEIRRIRTGKRPSDSSLSAPKAKKGPLDVMFMEKQTTSTRQTNIKDADKEARARTIQFIARFFYTNGIAFNVSRSKHFKEMLEAVGAYGHHLKPPSYYEIRGPLLQIELEETKKMLEPHLAARVNYGCSIMSDGWTDRKGRTLINFLVNSTAGTMFVKSIDASEESKTGEMVYKLMSDFVDEMGEQNVVQLITDNGSNYVNAGKKLMQNKPHIFWTPCAAHCIDLMLEDIGKIPRIKKAIQKGCSLVGYISNHTFALSLMRKKTNNVELVKHGVTRFATTFLTLQRLHKIKSKLREMFLCEEWLSSKCNKEAKGRQAATCALQPSFWNDVLHTLKSMAPLVDVLRMVDNEKKPAMGQYKDVFKIIDKRWECQLHHPLHAAGYYLNPKYFYSKPDIEKDPKLLRAEWWMNYGAETPDLTLMAIKVLSLVCSASGCERNWSTFEHIHSKKRSKLEHQKLQELVFIKYNQALKDRYDCLDKIDPVMLYDFNYHTEWLTGEMGEMGEDGEPVEEFVHENDDLTWDQVANASGVNEPIVNTRASMRAERAKELAAAASSNRASTSIRQEVFVEDVVGIEEEDDDDN*