AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0997340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGTCTGTGTGTTGGTTTC+TGG 0.016885 6_4:-76926234 Msa0997340:five_prime_UTR
TACAAGATGGATACTGATTT+TGG 0.200181 6_4:-76922802 Msa0997340:three_prime_UTR
ATTGCGCGCGTTGGAGTGTT+TGG 0.249256 6_4:-76922959 Msa0997340:CDS
CTGCTGGCAGCAGCAACAAC+TGG 0.256395 6_4:+76922997 None:intergenic
CTACATGATGAAGGCTAGTT+TGG 0.264669 6_4:+76926388 None:intergenic
TTTGCGGTTTCGTCGGATCT+TGG 0.277905 6_4:-76926262 Msa0997340:five_prime_UTR
GCCAGCAGCTGCTGTGGTGC+AGG 0.303418 6_4:-76922982 Msa0997340:CDS
TTGCGCGCGTTGGAGTGTTT+GGG 0.320307 6_4:-76922958 Msa0997340:CDS
TCCTGCACCACAGCAGCTGC+TGG 0.329371 6_4:+76922981 None:intergenic
CCTTCATCATGTAGATCCTT+TGG 0.355248 6_4:-76926379 Msa0997340:five_prime_UTR
TTGCGGTTTCGTCGGATCTT+GGG 0.360644 6_4:-76926261 Msa0997340:five_prime_UTR
TGAAGGCTAGTTTGGATTTG+AGG 0.382941 6_4:+76926396 None:intergenic
TCTGTTTGAGTTGCTGCTGC+TGG 0.383598 6_4:-76923036 Msa0997340:CDS
TCTCTTGCTCCTGCTTGATT+CGG 0.386260 6_4:+76926209 None:intergenic
CTATCTTAGCCTCGGGGACT+AGG 0.401284 6_4:+76925573 None:intergenic
GTTTCGTCGGATCTTGGGTT+TGG 0.402535 6_4:-76926256 Msa0997340:five_prime_UTR
CCAATCATTCATTCACGAAA+AGG 0.404191 6_4:-76926174 Msa0997340:five_prime_UTR
GCTGCTGCCAGCAGCTGCTG+TGG 0.406003 6_4:-76922988 Msa0997340:CDS
CGTTCTTCTTCCTCACGAAT+CGG 0.410511 6_4:+76926116 None:intergenic
CTGACGGAGAGAAGTTGATC+TGG 0.430791 6_4:+76926294 None:intergenic
TTCTTCCTCACGAATCGGTC+GGG 0.436012 6_4:+76926121 None:intergenic
TTCTTCAACTATCTTAGCCT+CGG 0.436680 6_4:+76925565 None:intergenic
TTACCTCTTTCGATTCTGTT+CGG 0.438439 6_4:+76926064 None:intergenic
TACATGGGCCTTCTTAGAAC+GGG 0.442047 6_4:+76925523 None:intergenic
TCCTTTGGCTGGTGGTGCGA+TGG 0.445597 6_4:-76926364 Msa0997340:five_prime_UTR
TGGGGTTGTTTGTAGAGAGA+AGG 0.452266 6_4:+76926442 None:intergenic
AGCAGGAGCAAGAGAGGAAG+TGG 0.456569 6_4:-76926201 Msa0997340:five_prime_UTR
GGCTAAGATAGTTGAAGAAA+AGG 0.464616 6_4:-76925561 Msa0997340:five_prime_UTR
TGCGCGCGTTGGAGTGTTTG+GGG 0.465976 6_4:-76922957 Msa0997340:CDS
AAGAAGGACGACGAAGAACT+AGG 0.471495 6_4:+76926317 None:intergenic
TGCTGTGGTGTGGTATGCGT+AGG 0.480584 6_4:-76922923 Msa0997340:three_prime_UTR
GGTTGTTTGTAGAGAGAAGG+AGG 0.486645 6_4:+76926445 None:intergenic
CATCATGTAGATCCTTTGGC+TGG 0.487300 6_4:-76926375 Msa0997340:five_prime_UTR
TCTTCAACTATCTTAGCCTC+GGG 0.489677 6_4:+76925566 None:intergenic
AGAAGGACGACGAAGAACTA+GGG 0.489999 6_4:+76926318 None:intergenic
AACAACACAAACGTTCAAAT+CGG 0.490303 6_4:-76926151 Msa0997340:five_prime_UTR
GGGTTTGGCTGTCTGTGTGT+TGG 0.497111 6_4:-76926241 Msa0997340:five_prime_UTR
AACTAGGGGCGCCGGTGGTG+CGG 0.500016 6_4:+76926333 None:intergenic
CTTCTTCCTCACGAATCGGT+CGG 0.503463 6_4:+76926120 None:intergenic
CCTTTGGCTGGTGGTGCGAT+GGG 0.513598 6_4:-76926363 Msa0997340:five_prime_UTR
GCTGTGGTGTGGTATGCGTA+GGG 0.524060 6_4:-76922922 Msa0997340:three_prime_UTR
AGGCTAGTTTGGATTTGAGG+AGG 0.528786 6_4:+76926399 None:intergenic
AGAAAAGGAAAACGATAAGA+AGG 0.537441 6_4:-76925546 Msa0997340:five_prime_UTR
TTACATGGGCCTTCTTAGAA+CGG 0.537464 6_4:+76925522 None:intergenic
TAAGACCCAAATTGGACTTG+TGG 0.537862 6_4:-76926510 None:intergenic
GTTGTAGATGCAATACAAGA+TGG 0.538406 6_4:-76922815 Msa0997340:three_prime_UTR
TGGGTTCTTCTCCGCACCAC+CGG 0.538577 6_4:-76926344 Msa0997340:five_prime_UTR
GAATCAAGCAGGAGCAAGAG+AGG 0.542256 6_4:-76926207 Msa0997340:five_prime_UTR
TGGTGCAGGATTGCGCGCGT+TGG 0.542643 6_4:-76922968 Msa0997340:CDS
CTGGGTTTGATGCTGTGGTG+TGG 0.544069 6_4:-76922933 Msa0997340:exon
TAAGAAGGCCCGTTCTAAGA+AGG 0.544367 6_4:-76925531 Msa0997340:five_prime_UTR
CCAAAGGATCTACATGATGA+AGG 0.552314 6_4:+76926379 None:intergenic
AGAGAAGTTGATCTGGAAGA+AGG 0.561043 6_4:+76926301 None:intergenic
TTTCTGGAACCGAATCAAGC+AGG 0.568528 6_4:-76926218 Msa0997340:five_prime_UTR
AAACCGAACAGAATCGAAAG+AGG 0.586011 6_4:-76926067 Msa0997340:intron
CTCAACGAACCAAACACACC+CGG 0.588644 6_4:+76922871 None:intergenic
CGAAGAACTAGGGGCGCCGG+TGG 0.588784 6_4:+76926328 None:intergenic
CGACGAAGAACTAGGGGCGC+CGG 0.593269 6_4:+76926325 None:intergenic
CATGTAGATCCTTTGGCTGG+TGG 0.594338 6_4:-76926372 Msa0997340:five_prime_UTR
CTGTGGTGTGGTATGCGTAG+GGG 0.597558 6_4:-76922921 Msa0997340:three_prime_UTR
TGTGGTGTGGTATGCGTAGG+GGG 0.602425 6_4:-76922920 Msa0997340:three_prime_UTR
GCGCGCGTTGGAGTGTTTGG+GGG 0.603953 6_4:-76922956 Msa0997340:CDS
CCCATCGCACCACCAGCCAA+AGG 0.639211 6_4:+76926363 None:intergenic
GAAGGACGACGAAGAACTAG+GGG 0.647554 6_4:+76926319 None:intergenic
CGATTCGTGAGGAAGAAGAA+CGG 0.665989 6_4:-76926115 Msa0997340:five_prime_UTR
TAGATCCCGACCGATTCGTG+AGG 0.670906 6_4:-76926126 Msa0997340:five_prime_UTR
CTTCAACTATCTTAGCCTCG+GGG 0.692932 6_4:+76925567 None:intergenic
TGAACGTTGCCTAGTCCCCG+AGG 0.707762 6_4:-76925582 Msa0997340:five_prime_UTR
GCAACTCAAACAGAAGACTG+CGG 0.738050 6_4:+76923045 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAACATGTATAAAAATT+TGG - chr6_4:76924656-76924675 Msa0997340:intron 10.0%
!!! ATTTATTTTTTCACTTTTTA+TGG - chr6_4:76924806-76924825 Msa0997340:intron 10.0%
!!! TTTATTTTTTCACTTTTTAT+GGG - chr6_4:76924807-76924826 Msa0997340:intron 10.0%
!! TAATAGTTAATGATAAAAAA+AGG - chr6_4:76924945-76924964 Msa0997340:intron 10.0%
!! AATAGTATACTATTAAATTA+TGG - chr6_4:76926069-76926088 Msa0997340:five_prime_UTR 10.0%
!!! CATGTGTTATTATTTTTAAA+TGG - chr6_4:76923475-76923494 Msa0997340:intron 15.0%
!! TTAGAAAATAGATAATTCAT+GGG - chr6_4:76924316-76924335 Msa0997340:intron 15.0%
!!! AATGATAAAAAAAGGTTTTA+TGG - chr6_4:76924953-76924972 Msa0997340:intron 15.0%
!!! TTACTAGAAAAATTTCTTTT+GGG + chr6_4:76925436-76925455 None:intergenic 15.0%
!!! AGTTATATTAAAGATTTTGA+GGG + chr6_4:76925527-76925546 None:intergenic 15.0%
!!! AAGTTATATTAAAGATTTTG+AGG + chr6_4:76925528-76925547 None:intergenic 15.0%
!! ACACAAGTAAAAAATTACAT+GGG + chr6_4:76923765-76923784 None:intergenic 20.0%
!!! TTTCTTTTAACTTTTGAGAA+AGG - chr6_4:76924072-76924091 Msa0997340:intron 20.0%
!! GTTAGAAAATAGATAATTCA+TGG - chr6_4:76924315-76924334 Msa0997340:intron 20.0%
!! ACTTCAAATAGGAAATTTAT+AGG + chr6_4:76924760-76924779 None:intergenic 20.0%
!! ATCAAAGAAAAACTAAAACT+AGG + chr6_4:76924788-76924807 None:intergenic 20.0%
!! ATTATCATATAGAATTGAAC+AGG - chr6_4:76925211-76925230 Msa0997340:intron 20.0%
!!! AGGTACTAAATTATCTATTT+TGG - chr6_4:76925374-76925393 Msa0997340:intron 20.0%
!!! GTTACTAGAAAAATTTCTTT+TGG + chr6_4:76925437-76925456 None:intergenic 20.0%
!! TATAATAACTAGAGATATAG+TGG + chr6_4:76925691-76925710 None:intergenic 20.0%
!!! AGAACGATTATTTTAGAAAG+AGG + chr6_4:76922805-76922824 None:intergenic 25.0%
! TGTTTCATTGTTGTATCTAA+TGG - chr6_4:76923334-76923353 Msa0997340:intron 25.0%
! AGCTAAACAAGTTTGAATTT+CGG + chr6_4:76923532-76923551 None:intergenic 25.0%
! GACACAAGTAAAAAATTACA+TGG + chr6_4:76923766-76923785 None:intergenic 25.0%
!! TTTTCTATAAGTGATTGAGT+TGG - chr6_4:76923787-76923806 Msa0997340:intron 25.0%
!! CAATGATTTGGAGATAAATT+AGG - chr6_4:76923817-76923836 Msa0997340:intron 25.0%
! TTGGAGATAAATTAGGTATT+TGG - chr6_4:76923824-76923843 Msa0997340:intron 25.0%
!!! TGTTTTTTGTTCATCTGTTT+TGG - chr6_4:76923963-76923982 Msa0997340:intron 25.0%
!!! GTTTTTTGTTCATCTGTTTT+GGG - chr6_4:76923964-76923983 Msa0997340:intron 25.0%
! CAGAAAAATTACTCAAACTT+GGG + chr6_4:76924129-76924148 None:intergenic 25.0%
! AGCAATGAATTCAATTTACT+AGG - chr6_4:76924188-76924207 Msa0997340:intron 25.0%
! TCATTAATTGTTTCCTGTTT+AGG - chr6_4:76924231-76924250 Msa0997340:intron 25.0%
!!! TTCCTGTTTAGGATTTTTAT+AGG - chr6_4:76924242-76924261 Msa0997340:intron 25.0%
! TACCTATAAAAATCCTAAAC+AGG + chr6_4:76924247-76924266 None:intergenic 25.0%
!! GATTTTTATAGGTAGACTTA+TGG - chr6_4:76924253-76924272 Msa0997340:intron 25.0%
!! TGTGGTATTTTCAATCAAAA+TGG + chr6_4:76924362-76924381 None:intergenic 25.0%
! AGCAAAATAATAAGTCTACT+CGG + chr6_4:76924628-76924647 None:intergenic 25.0%
! AAGAAAAACTAAAACTAGGT+GGG + chr6_4:76924784-76924803 None:intergenic 25.0%
! AAAGAAAAACTAAAACTAGG+TGG + chr6_4:76924785-76924804 None:intergenic 25.0%
! AAAAAAAACTTACAGTAGGT+AGG - chr6_4:76924830-76924849 Msa0997340:intron 25.0%
!! TATTTTACCTTCTATAGAGT+TGG + chr6_4:76924900-76924919 None:intergenic 25.0%
! GTATCAATCTATATAACTGA+TGG - chr6_4:76924983-76925002 Msa0997340:intron 25.0%
!! TATTCTTTTCGTCAACAAAA+GGG + chr6_4:76925163-76925182 None:intergenic 25.0%
!! ATATTCTTTTCGTCAACAAA+AGG + chr6_4:76925164-76925183 None:intergenic 25.0%
! CGAAAAGAATATCATGAAAT+TGG - chr6_4:76925172-76925191 Msa0997340:intron 25.0%
!!! TTTTTCTAGTAACTAGAGAA+TGG - chr6_4:76925444-76925463 Msa0997340:intron 25.0%
!!! TTTTCTAGTAACTAGAGAAT+GGG - chr6_4:76925445-76925464 Msa0997340:intron 25.0%
! GTTATGTTATTGATATCACA+TGG - chr6_4:76925803-76925822 Msa0997340:intron 25.0%
!! TTTGCAATGATCACTATTTT+AGG + chr6_4:76925839-76925858 None:intergenic 25.0%
! TATTAATAAGTAGACTGATC+AGG - chr6_4:76925863-76925882 Msa0997340:intron 25.0%
! AAAGTATATGTGATCCAAAT+GGG + chr6_4:76925921-76925940 None:intergenic 25.0%
! TCAAGTAATGATTGTCTAAT+AGG + chr6_4:76925975-76925994 None:intergenic 25.0%
! GCATTTGTTTAACAACAAAT+TGG + chr6_4:76926036-76926055 None:intergenic 25.0%
AACAACACAAACGTTCAAAT+CGG - chr6_4:76923119-76923138 Msa0997340:intron 30.0%
CGAAAAAAAGTTTGAACAGA+AGG + chr6_4:76923262-76923281 None:intergenic 30.0%
TAATGGAACCATTGTACAAA+GGG - chr6_4:76923351-76923370 Msa0997340:intron 30.0%
TGAGATAAGACATATTGCAA+AGG + chr6_4:76923581-76923600 None:intergenic 30.0%
TTCACTTTCAGAAAATGCAA+TGG - chr6_4:76923613-76923632 Msa0997340:intron 30.0%
AGAAAAGGAAAACGATAAGA+AGG - chr6_4:76923724-76923743 Msa0997340:intron 30.0%
TATCTCCAAATCATTGATCA+AGG + chr6_4:76923813-76923832 None:intergenic 30.0%
CCAGAAAAATTACTCAAACT+TGG + chr6_4:76924130-76924149 None:intergenic 30.0%
! CCAAGTTTGAGTAATTTTTC+TGG - chr6_4:76924127-76924146 Msa0997340:intron 30.0%
CATCAATTAGACATGAAGTT+AGG - chr6_4:76924152-76924171 Msa0997340:intron 30.0%
! TTTTCACTTACTGCATATAC+AGG - chr6_4:76924518-76924537 Msa0997340:intron 30.0%
! ATAAGTGTTGCAGAAAATGT+TGG - chr6_4:76924575-76924594 Msa0997340:intron 30.0%
TGGGAAAAAAAACTTACAGT+AGG - chr6_4:76924826-76924845 Msa0997340:intron 30.0%
!! TTGATATGGTGGATTTATGA+AGG + chr6_4:76924862-76924881 None:intergenic 30.0%
!!! TGTTTTAGACTCTAAAGCTA+AGG - chr6_4:76925111-76925130 Msa0997340:intron 30.0%
CTCTATTAAGATCTGCTAAT+CGG + chr6_4:76925621-76925640 None:intergenic 30.0%
TGCTTATGTAGCTTTCTATA+AGG - chr6_4:76925741-76925760 Msa0997340:intron 30.0%
CAATAACATAACAGATTGAC+AGG + chr6_4:76925795-76925814 None:intergenic 30.0%
! GAAAGTATATGTGATCCAAA+TGG + chr6_4:76925922-76925941 None:intergenic 30.0%
CATTACTTGAGAAATAGTAG+TGG - chr6_4:76925985-76926004 Msa0997340:intron 30.0%
! ATTGTTGTTGTCTTCTGTTT+CGG - chr6_4:76926168-76926187 Msa0997340:five_prime_UTR 30.0%
!!! TACAAGATGGATACTGATTT+TGG - chr6_4:76926468-76926487 Msa0997340:five_prime_UTR 30.0%
! CCTTTTCGTGAATGAATGAT+TGG + chr6_4:76923099-76923118 None:intergenic 35.0%
CCAATCATTCATTCACGAAA+AGG - chr6_4:76923096-76923115 Msa0997340:intron 35.0%
TTACCTCTTTCGATTCTGTT+CGG + chr6_4:76923209-76923228 None:intergenic 35.0%
GAATCGAAAGAGGTAAATAG+AGG - chr6_4:76923213-76923232 Msa0997340:intron 35.0%
AAAGAGACTAGATCTAGGTT+TGG + chr6_4:76923290-76923309 None:intergenic 35.0%
CTTTCAAAGAGACTAGATCT+AGG + chr6_4:76923295-76923314 None:intergenic 35.0%
! GATCTAGTCTCTTTGAAAGT+TGG - chr6_4:76923296-76923315 Msa0997340:intron 35.0%
CTAATGGAACCATTGTACAA+AGG - chr6_4:76923350-76923369 Msa0997340:intron 35.0%
TTTCCTATCCCTTTGTACAA+TGG + chr6_4:76923362-76923381 None:intergenic 35.0%
TACACTCACCTTGAAACAAT+TGG - chr6_4:76923427-76923446 Msa0997340:intron 35.0%
CATGTCAACCAATTGTTTCA+AGG + chr6_4:76923438-76923457 None:intergenic 35.0%
CTTGAAACAATTGGTTGACA+TGG - chr6_4:76923436-76923455 Msa0997340:intron 35.0%
TTCTTCAACTATCTTAGCCT+CGG + chr6_4:76923708-76923727 None:intergenic 35.0%
GGCTAAGATAGTTGAAGAAA+AGG - chr6_4:76923709-76923728 Msa0997340:intron 35.0%
AGTCATTGACAGTTATGTTG+AGG - chr6_4:76923892-76923911 Msa0997340:intron 35.0%
TTTCATCGTTTGTGACCTAT+AGG - chr6_4:76924434-76924453 Msa0997340:intron 35.0%
TATACAGGATGTAGTAGTGA+TGG - chr6_4:76924533-76924552 Msa0997340:intron 35.0%
!! AAAATTTGGTGCAGCAAAAG+AGG - chr6_4:76924670-76924689 Msa0997340:intron 35.0%
CAAAAGAGGAGTCATTTCTT+TGG - chr6_4:76924684-76924703 Msa0997340:intron 35.0%
AGGAGTTCATACGTTATCTT+AGG - chr6_4:76924720-76924739 Msa0997340:intron 35.0%
GGAGTTCATACGTTATCTTA+GGG - chr6_4:76924721-76924740 Msa0997340:intron 35.0%
! GAAGCTTTCAAGCTTTGATA+TGG + chr6_4:76924876-76924895 None:intergenic 35.0%
TAATGATGCAGTTACTGTTG+TGG - chr6_4:76925012-76925031 Msa0997340:intron 35.0%
AAATGAAGATGATTCATCCG+AGG - chr6_4:76925063-76925082 Msa0997340:intron 35.0%
GCTTTGTAGCACTTGTAAAT+AGG + chr6_4:76925091-76925110 None:intergenic 35.0%
! CTTTTGCATATTGTTGCCAT+GGG + chr6_4:76925286-76925305 None:intergenic 35.0%
! ACTTTTGCATATTGTTGCCA+TGG + chr6_4:76925287-76925306 None:intergenic 35.0%
TGGCAACAATATGCAAAAGT+CGG - chr6_4:76925287-76925306 Msa0997340:intron 35.0%
CTACGACAGTGTAACTAAAA+TGG + chr6_4:76925325-76925344 None:intergenic 35.0%
! TGAGGGAATGATGATTTTTG+AGG + chr6_4:76925510-76925529 None:intergenic 35.0%
TAGTTACAAGTTAGCCCATT+TGG - chr6_4:76925904-76925923 Msa0997340:intron 35.0%
!!! TATTGTCCGGTTGTTTTTTC+CGG - chr6_4:76926380-76926399 Msa0997340:five_prime_UTR 35.0%
GTTGTAGATGCAATACAAGA+TGG - chr6_4:76926455-76926474 Msa0997340:five_prime_UTR 35.0%
! AGAAAAAGTGAGGTCGGTTT+GGG + chr6_4:76922850-76922869 None:intergenic 40.0%
AAGAAAAAGTGAGGTCGGTT+TGG + chr6_4:76922851-76922870 None:intergenic 40.0%
TTGAGGAGGAAGAAAAAGTG+AGG + chr6_4:76922860-76922879 None:intergenic 40.0%
!! TGAAGGCTAGTTTGGATTTG+AGG + chr6_4:76922877-76922896 None:intergenic 40.0%
! CTACATGATGAAGGCTAGTT+TGG + chr6_4:76922885-76922904 None:intergenic 40.0%
CCAAAGGATCTACATGATGA+AGG + chr6_4:76922894-76922913 None:intergenic 40.0%
CCTTCATCATGTAGATCCTT+TGG - chr6_4:76922891-76922910 Msa0997340:three_prime_UTR 40.0%
! AGAGAAGTTGATCTGGAAGA+AGG + chr6_4:76922972-76922991 None:intergenic 40.0%
CCGCAAAAACTCAAATCTGA+CGG + chr6_4:76922995-76923014 None:intergenic 40.0%
!! CCGTCAGATTTGAGTTTTTG+CGG - chr6_4:76922992-76923011 Msa0997340:CDS 40.0%
! TTGAGTTTTTGCGGTTTCGT+CGG - chr6_4:76923001-76923020 Msa0997340:CDS 40.0%
AAACCGAACAGAATCGAAAG+AGG - chr6_4:76923203-76923222 Msa0997340:intron 40.0%
GAACCATTGTACAAAGGGAT+AGG - chr6_4:76923356-76923375 Msa0997340:intron 40.0%
TGTTGTAGTTGTCATGACTG+TGG - chr6_4:76923388-76923407 Msa0997340:intron 40.0%
GACATGGCAAGTGAAGATAA+AGG - chr6_4:76923452-76923471 Msa0997340:intron 40.0%
!! GAGCTCTAAGAAGTCTACAA+AGG + chr6_4:76923661-76923680 None:intergenic 40.0%
TCTTCAACTATCTTAGCCTC+GGG + chr6_4:76923707-76923726 None:intergenic 40.0%
TTACATGGGCCTTCTTAGAA+CGG + chr6_4:76923751-76923770 None:intergenic 40.0%
!! GTTGGCCTTGATCAATGATT+TGG - chr6_4:76923805-76923824 Msa0997340:intron 40.0%
AAAGCACATGCCCTTTCTAA+TGG - chr6_4:76923863-76923882 Msa0997340:intron 40.0%
AAGCACATGCCCTTTCTAAT+GGG - chr6_4:76923864-76923883 Msa0997340:intron 40.0%
GACAGTTATGTTGAGGAATC+AGG - chr6_4:76923899-76923918 Msa0997340:intron 40.0%
!!! TTGTTCATCTGTTTTGGGAG+AGG - chr6_4:76923969-76923988 Msa0997340:intron 40.0%
CAAAAGTGAACACTATGCTG+TGG + chr6_4:76924380-76924399 None:intergenic 40.0%
! CTGGCACTTTGAAAACCTAT+AGG + chr6_4:76924452-76924471 None:intergenic 40.0%
!! ATAGGTTTTCAAAGTGCCAG+AGG - chr6_4:76924452-76924471 Msa0997340:intron 40.0%
! TCTTTGGCGTGGAAATTTTG+AGG - chr6_4:76924700-76924719 Msa0997340:intron 40.0%
CATACGTTATCTTAGGGACA+AGG - chr6_4:76924727-76924746 Msa0997340:intron 40.0%
ACTAGGTGGGTACTTCAAAT+AGG + chr6_4:76924771-76924790 None:intergenic 40.0%
! GCTTTCAAGCTTTGATATGG+TGG + chr6_4:76924873-76924892 None:intergenic 40.0%
GCACTTGTAAATAGGATCCT+CGG + chr6_4:76925083-76925102 None:intergenic 40.0%
AACAGGCAATGCAAGCTAAT+TGG - chr6_4:76925228-76925247 Msa0997340:intron 40.0%
ACAGGCAATGCAAGCTAATT+GGG - chr6_4:76925229-76925248 Msa0997340:intron 40.0%
GGTGTCTTCAGTCTAAATTC+AGG - chr6_4:76925354-76925373 Msa0997340:intron 40.0%
!! GGGAATGATGATTTTTGAGG+CGG + chr6_4:76925507-76925526 None:intergenic 40.0%
AACAGATTGACAGGTAACCT+TGG + chr6_4:76925786-76925805 None:intergenic 40.0%
!!! GGTTTTTTGGTGTTGATAGC+AGG - chr6_4:76926201-76926220 Msa0997340:five_prime_UTR 40.0%
GGAAAAAACAACCGGACAAT+AGG + chr6_4:76926381-76926400 None:intergenic 40.0%
!!! ATTGTCCGGTTGTTTTTTCC+GGG - chr6_4:76926381-76926400 Msa0997340:five_prime_UTR 40.0%
!!! TTGTTTTTTCCGGGTGTGTT+TGG - chr6_4:76926390-76926409 Msa0997340:five_prime_UTR 40.0%
GGTTGTTTGTAGAGAGAAGG+AGG + chr6_4:76922828-76922847 None:intergenic 45.0%
TGGGGTTGTTTGTAGAGAGA+AGG + chr6_4:76922831-76922850 None:intergenic 45.0%
!! GAAAAAGTGAGGTCGGTTTG+GGG + chr6_4:76922849-76922868 None:intergenic 45.0%
GGAGGAAGAAAAAGTGAGGT+CGG + chr6_4:76922856-76922875 None:intergenic 45.0%
!! AGGCTAGTTTGGATTTGAGG+AGG + chr6_4:76922874-76922893 None:intergenic 45.0%
CATCATGTAGATCCTTTGGC+TGG - chr6_4:76922895-76922914 Msa0997340:three_prime_UTR 45.0%
AGAAGGACGACGAAGAACTA+GGG + chr6_4:76922955-76922974 None:intergenic 45.0%
AAGAAGGACGACGAAGAACT+AGG + chr6_4:76922956-76922975 None:intergenic 45.0%
!! TTTCTGGAACCGAATCAAGC+AGG - chr6_4:76923052-76923071 Msa0997340:intron 45.0%
TCTCTTGCTCCTGCTTGATT+CGG + chr6_4:76923064-76923083 None:intergenic 45.0%
CGTTCTTCTTCCTCACGAAT+CGG + chr6_4:76923157-76923176 None:intergenic 45.0%
CGATTCGTGAGGAAGAAGAA+CGG - chr6_4:76923155-76923174 Msa0997340:intron 45.0%
CTTCAACTATCTTAGCCTCG+GGG + chr6_4:76923706-76923725 None:intergenic 45.0%
!! TAAGAAGGCCCGTTCTAAGA+AGG - chr6_4:76923739-76923758 Msa0997340:intron 45.0%
TACATGGGCCTTCTTAGAAC+GGG + chr6_4:76923750-76923769 None:intergenic 45.0%
AGCACATGCCCTTTCTAATG+GGG - chr6_4:76923865-76923884 Msa0997340:intron 45.0%
GACTGAACCCCCATTAGAAA+GGG + chr6_4:76923876-76923895 None:intergenic 45.0%
TGACTGAACCCCCATTAGAA+AGG + chr6_4:76923877-76923896 None:intergenic 45.0%
!! GAGGAATCAGGAAGCATTGT+TGG - chr6_4:76923911-76923930 Msa0997340:intron 45.0%
AGCTTCGCCAACTCTATAGA+AGG - chr6_4:76924890-76924909 Msa0997340:intron 45.0%
TGGCAGCTTCTTGAGTATCT+TGG + chr6_4:76925257-76925276 None:intergenic 45.0%
GATACTCAAGAAGCTGCCAT+AGG - chr6_4:76925258-76925277 Msa0997340:intron 45.0%
ATTGTTGCCATGGGATCCTA+TGG + chr6_4:76925277-76925296 None:intergenic 45.0%
TACACTGTCGTAGCACTACA+TGG - chr6_4:76925332-76925351 Msa0997340:intron 45.0%
ACACTGTCGTAGCACTACAT+GGG - chr6_4:76925333-76925352 Msa0997340:intron 45.0%
CATCTCATCATTGAGCTCCA+AGG - chr6_4:76925766-76925785 Msa0997340:intron 45.0%
AAGTAGACTGATCAGGCGTA+TGG - chr6_4:76925870-76925889 Msa0997340:intron 45.0%
GCAACTCAAACAGAAGACTG+CGG + chr6_4:76926228-76926247 None:intergenic 45.0%
ACACACCCGGAAAAAACAAC+CGG + chr6_4:76926389-76926408 None:intergenic 45.0%
CATGTAGATCCTTTGGCTGG+TGG - chr6_4:76922898-76922917 Msa0997340:three_prime_UTR 50.0%
GAAGGACGACGAAGAACTAG+GGG + chr6_4:76922954-76922973 None:intergenic 50.0%
! CTGACGGAGAGAAGTTGATC+TGG + chr6_4:76922979-76922998 None:intergenic 50.0%
TTTGCGGTTTCGTCGGATCT+TGG - chr6_4:76923008-76923027 Msa0997340:CDS 50.0%
TTGCGGTTTCGTCGGATCTT+GGG - chr6_4:76923009-76923028 Msa0997340:CDS 50.0%
GTTTCGTCGGATCTTGGGTT+TGG - chr6_4:76923014-76923033 Msa0997340:CDS 50.0%
!! GCTGTCTGTGTGTTGGTTTC+TGG - chr6_4:76923036-76923055 Msa0997340:CDS 50.0%
GAATCAAGCAGGAGCAAGAG+AGG - chr6_4:76923063-76923082 Msa0997340:intron 50.0%
TTCTTCCTCACGAATCGGTC+GGG + chr6_4:76923152-76923171 None:intergenic 50.0%
CTTCTTCCTCACGAATCGGT+CGG + chr6_4:76923153-76923172 None:intergenic 50.0%
CATGACTGTGGTTGTCGTCA+TGG - chr6_4:76923400-76923419 Msa0997340:intron 50.0%
AGGCTCTCTCATACAGCATC+TGG + chr6_4:76923641-76923660 None:intergenic 50.0%
GCACATGCCCTTTCTAATGG+GGG - chr6_4:76923866-76923885 Msa0997340:intron 50.0%
TCCACAACACGATGTTCCTC+TGG + chr6_4:76924471-76924490 None:intergenic 50.0%
TTGTGGAAGCTGCAAAGCGT+GGG - chr6_4:76924484-76924503 Msa0997340:intron 50.0%
GAGGAGTCATTTCTTTGGCG+TGG - chr6_4:76924689-76924708 Msa0997340:intron 50.0%
TTCTGTTTCGGTCTGCTGTG+TGG - chr6_4:76926180-76926199 Msa0997340:five_prime_UTR 50.0%
!!! CGGTCTGCTGTGTGGTTTTT+TGG - chr6_4:76926188-76926207 Msa0997340:five_prime_UTR 50.0%
!! TCTGTTTGAGTTGCTGCTGC+TGG - chr6_4:76926234-76926253 Msa0997340:five_prime_UTR 50.0%
AGGGGGTCATTCCTATTGTC+CGG - chr6_4:76926367-76926386 Msa0997340:five_prime_UTR 50.0%
CTCAACGAACCAAACACACC+CGG + chr6_4:76926402-76926421 None:intergenic 50.0%
GGGTTTGGCTGTCTGTGTGT+TGG - chr6_4:76923029-76923048 Msa0997340:CDS 55.0%
AGCAGGAGCAAGAGAGGAAG+TGG - chr6_4:76923069-76923088 Msa0997340:intron 55.0%
!! TAGATCCCGACCGATTCGTG+AGG - chr6_4:76923144-76923163 Msa0997340:intron 55.0%
CTATCTTAGCCTCGGGGACT+AGG + chr6_4:76923700-76923719 None:intergenic 55.0%
GCCAGAGGAACATCGTGTTG+TGG - chr6_4:76924467-76924486 Msa0997340:intron 55.0%
GTTGTGGAAGCTGCAAAGCG+TGG - chr6_4:76924483-76924502 Msa0997340:intron 55.0%
GAAGCTGCCATAGGATCCCA+TGG - chr6_4:76925267-76925286 Msa0997340:intron 55.0%
! ATTGCGCGCGTTGGAGTGTT+TGG - chr6_4:76926311-76926330 Msa0997340:five_prime_UTR 55.0%
! TTGCGCGCGTTGGAGTGTTT+GGG - chr6_4:76926312-76926331 Msa0997340:five_prime_UTR 55.0%
CTGGGTTTGATGCTGTGGTG+TGG - chr6_4:76926337-76926356 Msa0997340:five_prime_UTR 55.0%
TGCTGTGGTGTGGTATGCGT+AGG - chr6_4:76926347-76926366 Msa0997340:five_prime_UTR 55.0%
GCTGTGGTGTGGTATGCGTA+GGG - chr6_4:76926348-76926367 Msa0997340:five_prime_UTR 55.0%
CTGTGGTGTGGTATGCGTAG+GGG - chr6_4:76926349-76926368 Msa0997340:five_prime_UTR 55.0%
TGTGGTGTGGTATGCGTAGG+GGG - chr6_4:76926350-76926369 Msa0997340:five_prime_UTR 55.0%
! TCCTTTGGCTGGTGGTGCGA+TGG - chr6_4:76922906-76922925 Msa0997340:three_prime_UTR 60.0%
! CCTTTGGCTGGTGGTGCGAT+GGG - chr6_4:76922907-76922926 Msa0997340:three_prime_UTR 60.0%
TGGGTTCTTCTCCGCACCAC+CGG - chr6_4:76922926-76922945 Msa0997340:three_prime_UTR 60.0%
TGAACGTTGCCTAGTCCCCG+AGG - chr6_4:76923688-76923707 Msa0997340:intron 60.0%
CTGCTGGCAGCAGCAACAAC+TGG + chr6_4:76926276-76926295 None:intergenic 60.0%
! TGCGCGCGTTGGAGTGTTTG+GGG - chr6_4:76926313-76926332 Msa0997340:five_prime_UTR 60.0%
! CGTTGGAGTGTTTGGGGGCT+GGG - chr6_4:76926319-76926338 Msa0997340:five_prime_UTR 60.0%
CCCATCGCACCACCAGCCAA+AGG + chr6_4:76922910-76922929 None:intergenic 65.0%
! CGACGAAGAACTAGGGGCGC+CGG + chr6_4:76922948-76922967 None:intergenic 65.0%
TCCTGCACCACAGCAGCTGC+TGG + chr6_4:76926292-76926311 None:intergenic 65.0%
TGGTGCAGGATTGCGCGCGT+TGG - chr6_4:76926302-76926321 Msa0997340:five_prime_UTR 65.0%
! GCGCGCGTTGGAGTGTTTGG+GGG - chr6_4:76926314-76926333 Msa0997340:five_prime_UTR 65.0%
! GCGTTGGAGTGTTTGGGGGC+TGG - chr6_4:76926318-76926337 Msa0997340:five_prime_UTR 65.0%
GGGGGCTGGGTTTGATGCTG+TGG - chr6_4:76926332-76926351 Msa0997340:five_prime_UTR 65.0%
!! AACTAGGGGCGCCGGTGGTG+CGG + chr6_4:76922940-76922959 None:intergenic 70.0%
!! CGAAGAACTAGGGGCGCCGG+TGG + chr6_4:76922945-76922964 None:intergenic 70.0%
GCTGCTGCCAGCAGCTGCTG+TGG - chr6_4:76926282-76926301 Msa0997340:five_prime_UTR 70.0%
GCCAGCAGCTGCTGTGGTGC+AGG - chr6_4:76926288-76926307 Msa0997340:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 76922773 76926519 76922773 ID=Msa0997340;Name=Msa0997340
chr6_4 mRNA 76922773 76926519 76922773 ID=Msa0997340-mRNA-1;Parent=Msa0997340;Name=Msa0997340-mRNA-1;_AED=0.22;_eAED=0.22;_QI=550|0.5|0.66|1|0|0.33|3|173|41
chr6_4 exon 76922773 76923069 76922773 ID=Msa0997340-mRNA-1:exon:14454;Parent=Msa0997340-mRNA-1
chr6_4 exon 76925526 76925625 76925526 ID=Msa0997340-mRNA-1:exon:14453;Parent=Msa0997340-mRNA-1
chr6_4 exon 76926068 76926519 76926068 ID=Msa0997340-mRNA-1:exon:14452;Parent=Msa0997340-mRNA-1
chr6_4 five_prime_UTR 76926068 76926519 76926068 ID=Msa0997340-mRNA-1:five_prime_utr;Parent=Msa0997340-mRNA-1
chr6_4 five_prime_UTR 76925528 76925625 76925528 ID=Msa0997340-mRNA-1:five_prime_utr;Parent=Msa0997340-mRNA-1
chr6_4 CDS 76925526 76925527 76925526 ID=Msa0997340-mRNA-1:cds;Parent=Msa0997340-mRNA-1
chr6_4 CDS 76922946 76923069 76922946 ID=Msa0997340-mRNA-1:cds;Parent=Msa0997340-mRNA-1
chr6_4 three_prime_UTR 76922773 76922945 76922773 ID=Msa0997340-mRNA-1:three_prime_utr;Parent=Msa0997340-mRNA-1
Gene Sequence

>Msa0997340

ATGTCCGCAGTCTTCTGTTTGAGTTGCTGCTGCTGGTTGTTTTCTACTGTCTCCAGTTGTTGCTGCTGCCAGCAGCTGCTGTGGTGCAGGATTGCGCGCGTTGGAGTGTTTGGGGGCTGGGTTTGA
Protein sequence

>Msa0997340

MSAVFCLSCCCWLFSTVSSCCCCQQLLWCRIARVGVFGGWV*