Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0725860 | Msa0999310 | 0.814576 | 1.436350e-51 | -8.615850e-47 |
| Msa0999310 | Msa1001880 | 0.973271 | 3.803500e-136 | -8.615850e-47 |
| Msa0999310 | Msa1456730 | 0.849582 | 2.947742e-60 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAAATCATCTTCATTT+TGG | 0.118652 | 6_4:+81248526 | Msa0999310:three_prime_UTR |
| TTTACACGTTGTTGGAGATT+TGG | 0.310389 | 6_4:+81248070 | Msa0999310:CDS |
| TACCTACCTCAGTTTATTGA+TGG | 0.361683 | 6_4:+81247990 | Msa0999310:intron |
| CTTGCTGCACAAACTTTGTT+GGG | 0.364287 | 6_4:-81248249 | None:intergenic |
| GTGCAGGGAGTGAGGGTTTG+AGG | 0.383807 | 6_4:-81248352 | None:intergenic |
| GCTTGCTGCACAAACTTTGT+TGG | 0.394409 | 6_4:-81248250 | None:intergenic |
| TTGGCAACTTGCACAGCAAA+AGG | 0.399101 | 6_4:-81248605 | None:intergenic |
| GATGATCCTTTACACGTTGT+TGG | 0.399617 | 6_4:+81248062 | Msa0999310:CDS |
| CCTGAGTCCTGACAACTTTG+AGG | 0.403698 | 6_4:-81248417 | None:intergenic |
| ATGACCAGAGCAACATCCTT+TGG | 0.404882 | 6_4:-81248289 | None:intergenic |
| CAAGGTCAACGGCACCATCT+TGG | 0.410802 | 6_4:-81248019 | None:intergenic |
| CCTCACTCCCTGCACCTACT+TGG | 0.449885 | 6_4:+81248360 | Msa0999310:CDS |
| AAGGTCAACGGCACCATCTT+GGG | 0.460028 | 6_4:-81248018 | None:intergenic |
| TGTTGCTCTGGTCATCGAAT+GGG | 0.463712 | 6_4:+81248297 | Msa0999310:CDS |
| TTCTCGTACCAAGTAGGTGC+AGG | 0.468618 | 6_4:-81248368 | None:intergenic |
| AAGGACCTCAATGTACATCT+TGG | 0.486848 | 6_4:+81248468 | Msa0999310:CDS |
| TGGCAACTTGCACAGCAAAA+GGG | 0.491951 | 6_4:-81248604 | None:intergenic |
| AAATCTCCAACAACGTGTAA+AGG | 0.509860 | 6_4:-81248068 | None:intergenic |
| AAAATGGCCTCAAAGTTGTC+AGG | 0.510936 | 6_4:+81248410 | Msa0999310:CDS |
| TAAGCCAAAGGATGTTGCTC+TGG | 0.513083 | 6_4:+81248285 | Msa0999310:CDS |
| GCACCTACTTGGTACGAGAA+TGG | 0.517142 | 6_4:+81248371 | Msa0999310:CDS |
| TGATGCACTCCCTGGTCTGA+AGG | 0.521175 | 6_4:+81248040 | Msa0999310:CDS |
| CCAAGTAGGTGCAGGGAGTG+AGG | 0.532064 | 6_4:-81248360 | None:intergenic |
| GTTGACCTTGATGCACTCCC+TGG | 0.532730 | 6_4:+81248032 | Msa0999310:CDS |
| GGCAACTTGCACAGCAAAAG+GGG | 0.534379 | 6_4:-81248603 | None:intergenic |
| ATGTTGCTCTGGTCATCGAA+TGG | 0.536036 | 6_4:+81248296 | Msa0999310:CDS |
| TATATGAGTGTGGCATCATA+CGG | 0.559711 | 6_4:+81248326 | Msa0999310:CDS |
| CCTCAAAGTTGTCAGGACTC+AGG | 0.563260 | 6_4:+81248417 | Msa0999310:CDS |
| CAAGTAGGTGCAGGGAGTGA+GGG | 0.566748 | 6_4:-81248359 | None:intergenic |
| ATTGATGGCTATGCCCAAGA+TGG | 0.573474 | 6_4:+81248005 | Msa0999310:CDS |
| GTTGCTCTGGTCATCGAATG+GGG | 0.587947 | 6_4:+81248298 | Msa0999310:CDS |
| AAAGGATCATCCTTCAGACC+AGG | 0.597057 | 6_4:-81248050 | None:intergenic |
| TCAGACCAGGGAGTGCATCA+AGG | 0.598884 | 6_4:-81248037 | None:intergenic |
| TCTCCATTCTCGTACCAAGT+AGG | 0.607950 | 6_4:-81248374 | None:intergenic |
| GCAAGTGACAGAGAATAAGA+TGG | 0.612366 | 6_4:+81248121 | Msa0999310:CDS |
| ACTCAGGAGGGTAAAGCTGA+CGG | 0.622875 | 6_4:+81248433 | Msa0999310:CDS |
| CAAAGTTGTCAGGACTCAGG+AGG | 0.634507 | 6_4:+81248420 | Msa0999310:CDS |
| TGAAACCAAGATGTACATTG+AGG | 0.640122 | 6_4:-81248473 | None:intergenic |
| TCTCGTACCAAGTAGGTGCA+GGG | 0.642066 | 6_4:-81248367 | None:intergenic |
| AGGGAGTGCATCAAGGTCAA+CGG | 0.646566 | 6_4:-81248030 | None:intergenic |
| CTGACGGCATATCTTGCGAA+AGG | 0.650466 | 6_4:+81248449 | Msa0999310:CDS |
| AAGGATCATCCTTCAGACCA+GGG | 0.655500 | 6_4:-81248049 | None:intergenic |
| AAAGTTGTCAGGACTCAGGA+GGG | 0.656543 | 6_4:+81248421 | Msa0999310:CDS |
| TTGCTCTGGTCATCGAATGG+GGG | 0.660188 | 6_4:+81248299 | Msa0999310:CDS |
| TCTGCTAGAGAATAAGCCAA+AGG | 0.665296 | 6_4:+81248273 | Msa0999310:CDS |
| ATTCTCTGTCACTTGCAATG+CGG | 0.692219 | 6_4:-81248114 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGTTGTAATCAATTAATA+AGG | - | chr6_4:81248186-81248205 | None:intergenic | 20.0% |
| !!! | AAGTTAAATCATCTTCATTT+TGG | + | chr6_4:81248526-81248545 | Msa0999310:three_prime_UTR | 20.0% |
| !!! | AGTTAAATCATCTTCATTTT+GGG | + | chr6_4:81248527-81248546 | Msa0999310:three_prime_UTR | 20.0% |
| ! | AGAATGGAGACATTTTTGTA+GGG | + | chr6_4:81248387-81248406 | Msa0999310:CDS | 30.0% |
| ! | AGACATTTTTGTAGGGAAAA+TGG | + | chr6_4:81248394-81248413 | Msa0999310:CDS | 30.0% |
| !!! | CTTCATTTTGGGTTAATATC+TGG | + | chr6_4:81248538-81248557 | Msa0999310:three_prime_UTR | 30.0% |
| !! | AGGTAGGTATTGATATATGG+CGG | - | chr6_4:81247979-81247998 | None:intergenic | 35.0% |
| !! | CTGAGGTAGGTATTGATATA+TGG | - | chr6_4:81247982-81248001 | None:intergenic | 35.0% |
| TACCTACCTCAGTTTATTGA+TGG | + | chr6_4:81247990-81248009 | Msa0999310:intron | 35.0% | |
| AAATCTCCAACAACGTGTAA+AGG | - | chr6_4:81248071-81248090 | None:intergenic | 35.0% | |
| TTTACACGTTGTTGGAGATT+TGG | + | chr6_4:81248070-81248089 | Msa0999310:CDS | 35.0% | |
| TATATGAGTGTGGCATCATA+CGG | + | chr6_4:81248326-81248345 | Msa0999310:CDS | 35.0% | |
| ! | GAGAATGGAGACATTTTTGT+AGG | + | chr6_4:81248386-81248405 | Msa0999310:CDS | 35.0% |
| TGAAACCAAGATGTACATTG+AGG | - | chr6_4:81248476-81248495 | None:intergenic | 35.0% | |
| AGATGTTAATTATGCCCACG+AGG | + | chr6_4:81247942-81247961 | Msa0999310:intron | 40.0% | |
| AGCCATCAATAAACTGAGGT+AGG | - | chr6_4:81247995-81248014 | None:intergenic | 40.0% | |
| GCATAGCCATCAATAAACTG+AGG | - | chr6_4:81247999-81248018 | None:intergenic | 40.0% | |
| GATGATCCTTTACACGTTGT+TGG | + | chr6_4:81248062-81248081 | Msa0999310:CDS | 40.0% | |
| ! | ATTCTCTGTCACTTGCAATG+CGG | - | chr6_4:81248117-81248136 | None:intergenic | 40.0% |
| GCAAGTGACAGAGAATAAGA+TGG | + | chr6_4:81248121-81248140 | Msa0999310:CDS | 40.0% | |
| CTTGCTGCACAAACTTTGTT+GGG | - | chr6_4:81248252-81248271 | None:intergenic | 40.0% | |
| TCTGCTAGAGAATAAGCCAA+AGG | + | chr6_4:81248273-81248292 | Msa0999310:CDS | 40.0% | |
| AAAATGGCCTCAAAGTTGTC+AGG | + | chr6_4:81248410-81248429 | Msa0999310:CDS | 40.0% | |
| AAGGACCTCAATGTACATCT+TGG | + | chr6_4:81248468-81248487 | Msa0999310:CDS | 40.0% | |
| CACAGCAAAAGGGGAAAAAA+CGG | - | chr6_4:81248597-81248616 | None:intergenic | 40.0% | |
| ! | ATTGATGGCTATGCCCAAGA+TGG | + | chr6_4:81248005-81248024 | Msa0999310:CDS | 45.0% |
| AAGGATCATCCTTCAGACCA+GGG | - | chr6_4:81248052-81248071 | None:intergenic | 45.0% | |
| AAAGGATCATCCTTCAGACC+AGG | - | chr6_4:81248053-81248072 | None:intergenic | 45.0% | |
| ! | GCTTGCTGCACAAACTTTGT+TGG | - | chr6_4:81248253-81248272 | None:intergenic | 45.0% |
| TAAGCCAAAGGATGTTGCTC+TGG | + | chr6_4:81248285-81248304 | Msa0999310:CDS | 45.0% | |
| ATGACCAGAGCAACATCCTT+TGG | - | chr6_4:81248292-81248311 | None:intergenic | 45.0% | |
| ATGTTGCTCTGGTCATCGAA+TGG | + | chr6_4:81248296-81248315 | Msa0999310:CDS | 45.0% | |
| TGTTGCTCTGGTCATCGAAT+GGG | + | chr6_4:81248297-81248316 | Msa0999310:CDS | 45.0% | |
| TGGGGGAATGTATATGAGTG+TGG | + | chr6_4:81248316-81248335 | Msa0999310:CDS | 45.0% | |
| TCTCCATTCTCGTACCAAGT+AGG | - | chr6_4:81248377-81248396 | None:intergenic | 45.0% | |
| AAAGTTGTCAGGACTCAGGA+GGG | + | chr6_4:81248421-81248440 | Msa0999310:CDS | 45.0% | |
| AAGGTCAACGGCACCATCTT+GGG | - | chr6_4:81248021-81248040 | None:intergenic | 50.0% | |
| ! | AGGGAGTGCATCAAGGTCAA+CGG | - | chr6_4:81248033-81248052 | None:intergenic | 50.0% |
| GTTGCTCTGGTCATCGAATG+GGG | + | chr6_4:81248298-81248317 | Msa0999310:CDS | 50.0% | |
| TTGCTCTGGTCATCGAATGG+GGG | + | chr6_4:81248299-81248318 | Msa0999310:CDS | 50.0% | |
| TCTCGTACCAAGTAGGTGCA+GGG | - | chr6_4:81248370-81248389 | None:intergenic | 50.0% | |
| TTCTCGTACCAAGTAGGTGC+AGG | - | chr6_4:81248371-81248390 | None:intergenic | 50.0% | |
| GCACCTACTTGGTACGAGAA+TGG | + | chr6_4:81248371-81248390 | Msa0999310:CDS | 50.0% | |
| CCTGAGTCCTGACAACTTTG+AGG | - | chr6_4:81248420-81248439 | None:intergenic | 50.0% | |
| CCTCAAAGTTGTCAGGACTC+AGG | + | chr6_4:81248417-81248436 | Msa0999310:CDS | 50.0% | |
| CAAAGTTGTCAGGACTCAGG+AGG | + | chr6_4:81248420-81248439 | Msa0999310:CDS | 50.0% | |
| ACTCAGGAGGGTAAAGCTGA+CGG | + | chr6_4:81248433-81248452 | Msa0999310:CDS | 50.0% | |
| CTGACGGCATATCTTGCGAA+AGG | + | chr6_4:81248449-81248468 | Msa0999310:CDS | 50.0% | |
| CAAGGTCAACGGCACCATCT+TGG | - | chr6_4:81248022-81248041 | None:intergenic | 55.0% | |
| GTTGACCTTGATGCACTCCC+TGG | + | chr6_4:81248032-81248051 | Msa0999310:CDS | 55.0% | |
| ! | TCAGACCAGGGAGTGCATCA+AGG | - | chr6_4:81248040-81248059 | None:intergenic | 55.0% |
| TGATGCACTCCCTGGTCTGA+AGG | + | chr6_4:81248040-81248059 | Msa0999310:CDS | 55.0% | |
| CAAGTAGGTGCAGGGAGTGA+GGG | - | chr6_4:81248362-81248381 | None:intergenic | 55.0% | |
| !! | ATGCCCACGAGGTGCTGATC+TGG | + | chr6_4:81247953-81247972 | Msa0999310:intron | 60.0% |
| GTGCAGGGAGTGAGGGTTTG+AGG | - | chr6_4:81248355-81248374 | None:intergenic | 60.0% | |
| CCAAGTAGGTGCAGGGAGTG+AGG | - | chr6_4:81248363-81248382 | None:intergenic | 60.0% | |
| CCTCACTCCCTGCACCTACT+TGG | + | chr6_4:81248360-81248379 | Msa0999310:CDS | 60.0% | |
| CGGCCAGATCAGCACCTCGT+GGG | - | chr6_4:81247959-81247978 | None:intergenic | 65.0% | |
| GCGGCCAGATCAGCACCTCG+TGG | - | chr6_4:81247960-81247979 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 81247937 | 81248623 | 81247937 | ID=Msa0999310;Name=Msa0999310 |
| chr6_4 | mRNA | 81247937 | 81248623 | 81247937 | ID=Msa0999310-mRNA-1;Parent=Msa0999310;Name=Msa0999310-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.5|0.33|0.66|1|1|3|113|138 |
| chr6_4 | exon | 81247937 | 81247945 | 81247937 | ID=Msa0999310-mRNA-1:exon:15524;Parent=Msa0999310-mRNA-1 |
| chr6_4 | exon | 81248002 | 81248142 | 81248002 | ID=Msa0999310-mRNA-1:exon:15525;Parent=Msa0999310-mRNA-1 |
| chr6_4 | exon | 81248244 | 81248623 | 81248244 | ID=Msa0999310-mRNA-1:exon:15526;Parent=Msa0999310-mRNA-1 |
| chr6_4 | CDS | 81247937 | 81247945 | 81247937 | ID=Msa0999310-mRNA-1:cds;Parent=Msa0999310-mRNA-1 |
| chr6_4 | CDS | 81248002 | 81248142 | 81248002 | ID=Msa0999310-mRNA-1:cds;Parent=Msa0999310-mRNA-1 |
| chr6_4 | CDS | 81248244 | 81248510 | 81248244 | ID=Msa0999310-mRNA-1:cds;Parent=Msa0999310-mRNA-1 |
| chr6_4 | three_prime_UTR | 81248511 | 81248623 | 81248511 | ID=Msa0999310-mRNA-1:three_prime_utr;Parent=Msa0999310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
