Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0725860 | Msa1001880 | 0.813059 | 3.099479e-51 | -8.615850e-47 |
| Msa0999310 | Msa1001880 | 0.973271 | 3.803500e-136 | -8.615850e-47 |
| Msa1001880 | Msa1456730 | 0.848209 | 7.090747e-60 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTTAAATCATCTTCATTT+TGG | 0.118652 | 6_4:-85983499 | Msa1001880:three_prime_UTR |
| TTTACACGTTGTTGGAGATT+TGG | 0.310389 | 6_4:-85983955 | Msa1001880:CDS |
| TACCTACCTCAGTTTATTGA+TGG | 0.361683 | 6_4:-85984035 | Msa1001880:intron |
| CTTGCTGCACAAACTTTGTT+GGG | 0.364287 | 6_4:+85983776 | None:intergenic |
| GTGCAGGGAGTGAGGGTTTG+AGG | 0.383807 | 6_4:+85983673 | None:intergenic |
| GCTTGCTGCACAAACTTTGT+TGG | 0.394409 | 6_4:+85983775 | None:intergenic |
| TTGGCAACTTGCACAGCAAA+AGG | 0.399101 | 6_4:+85983420 | None:intergenic |
| GATGATCCTTTACACGTTGT+TGG | 0.399617 | 6_4:-85983963 | Msa1001880:CDS |
| CCTGAGTCCTGACAACTTTG+AGG | 0.403698 | 6_4:+85983608 | None:intergenic |
| ATGACCAGAGCAACATCCTT+TGG | 0.404882 | 6_4:+85983736 | None:intergenic |
| CAAGGTCAACGGCACCATCT+TGG | 0.410802 | 6_4:+85984006 | None:intergenic |
| CCTCACTCCCTGCACCTACT+TGG | 0.449885 | 6_4:-85983665 | Msa1001880:CDS |
| AAGGTCAACGGCACCATCTT+GGG | 0.460028 | 6_4:+85984007 | None:intergenic |
| TGTTGCTCTGGTCATCGAAT+GGG | 0.463712 | 6_4:-85983728 | Msa1001880:CDS |
| TTCTCGTACCAAGTAGGTGC+AGG | 0.468618 | 6_4:+85983657 | None:intergenic |
| AAGGACCTCAATGTACATCT+TGG | 0.486848 | 6_4:-85983557 | Msa1001880:CDS |
| TGGCAACTTGCACAGCAAAA+GGG | 0.491951 | 6_4:+85983421 | None:intergenic |
| AAATCTCCAACAACGTGTAA+AGG | 0.509860 | 6_4:+85983957 | None:intergenic |
| AAAATGGCCTCAAAGTTGTC+AGG | 0.510936 | 6_4:-85983615 | Msa1001880:CDS |
| TAAGCCAAAGGATGTTGCTC+TGG | 0.513083 | 6_4:-85983740 | Msa1001880:CDS |
| GCACCTACTTGGTACGAGAA+TGG | 0.517142 | 6_4:-85983654 | Msa1001880:CDS |
| TGATGCACTCCCTGGTCTGA+AGG | 0.521175 | 6_4:-85983985 | Msa1001880:CDS |
| CCAAGTAGGTGCAGGGAGTG+AGG | 0.532064 | 6_4:+85983665 | None:intergenic |
| GTTGACCTTGATGCACTCCC+TGG | 0.532730 | 6_4:-85983993 | Msa1001880:CDS |
| GGCAACTTGCACAGCAAAAG+GGG | 0.534379 | 6_4:+85983422 | None:intergenic |
| ATGTTGCTCTGGTCATCGAA+TGG | 0.536036 | 6_4:-85983729 | Msa1001880:CDS |
| TATATGAGTGTGGCATCATA+CGG | 0.559711 | 6_4:-85983699 | Msa1001880:CDS |
| CCTCAAAGTTGTCAGGACTC+AGG | 0.563260 | 6_4:-85983608 | Msa1001880:CDS |
| CAAGTAGGTGCAGGGAGTGA+GGG | 0.566748 | 6_4:+85983666 | None:intergenic |
| ATTGATGGCTATGCCCAAGA+TGG | 0.573474 | 6_4:-85984020 | Msa1001880:CDS |
| GTTGCTCTGGTCATCGAATG+GGG | 0.587947 | 6_4:-85983727 | Msa1001880:CDS |
| AAAGGATCATCCTTCAGACC+AGG | 0.597057 | 6_4:+85983975 | None:intergenic |
| TCAGACCAGGGAGTGCATCA+AGG | 0.598884 | 6_4:+85983988 | None:intergenic |
| TCTCCATTCTCGTACCAAGT+AGG | 0.607950 | 6_4:+85983651 | None:intergenic |
| GCAAGTGACAGAGAATAAGA+TGG | 0.612366 | 6_4:-85983904 | Msa1001880:intron |
| ACTCAGGAGGGTAAAGCTGA+CGG | 0.622875 | 6_4:-85983592 | Msa1001880:CDS |
| CAAAGTTGTCAGGACTCAGG+AGG | 0.634507 | 6_4:-85983605 | Msa1001880:CDS |
| TGAAACCAAGATGTACATTG+AGG | 0.640122 | 6_4:+85983552 | None:intergenic |
| TCTCGTACCAAGTAGGTGCA+GGG | 0.642066 | 6_4:+85983658 | None:intergenic |
| AGGGAGTGCATCAAGGTCAA+CGG | 0.646566 | 6_4:+85983995 | None:intergenic |
| CTGACGGCATATCTTGCGAA+AGG | 0.650466 | 6_4:-85983576 | Msa1001880:CDS |
| AAGGATCATCCTTCAGACCA+GGG | 0.655500 | 6_4:+85983976 | None:intergenic |
| AAAGTTGTCAGGACTCAGGA+GGG | 0.656543 | 6_4:-85983604 | Msa1001880:CDS |
| TTGCTCTGGTCATCGAATGG+GGG | 0.660188 | 6_4:-85983726 | Msa1001880:CDS |
| TCTGCTAGAGAATAAGCCAA+AGG | 0.665296 | 6_4:-85983752 | Msa1001880:CDS |
| ATTCTCTGTCACTTGCAATG+CGG | 0.692219 | 6_4:+85983911 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGTTGTAATCAATTAATA+AGG | + | chr6_4:85983673-85983692 | None:intergenic | 20.0% |
| !!! | AAGTTAAATCATCTTCATTT+TGG | - | chr6_4:85984013-85984032 | Msa1001880:CDS | 20.0% |
| !!! | AGTTAAATCATCTTCATTTT+GGG | - | chr6_4:85984014-85984033 | Msa1001880:CDS | 20.0% |
| ! | AGAATGGAGACATTTTTGTA+GGG | - | chr6_4:85983874-85983893 | Msa1001880:intron | 30.0% |
| ! | AGACATTTTTGTAGGGAAAA+TGG | - | chr6_4:85983881-85983900 | Msa1001880:intron | 30.0% |
| !!! | CTTCATTTTGGGTTAATATC+TGG | - | chr6_4:85984025-85984044 | Msa1001880:CDS | 30.0% |
| !! | AGGTAGGTATTGATATATGG+CGG | + | chr6_4:85983466-85983485 | None:intergenic | 35.0% |
| !! | CTGAGGTAGGTATTGATATA+TGG | + | chr6_4:85983469-85983488 | None:intergenic | 35.0% |
| TACCTACCTCAGTTTATTGA+TGG | - | chr6_4:85983477-85983496 | Msa1001880:three_prime_UTR | 35.0% | |
| AAATCTCCAACAACGTGTAA+AGG | + | chr6_4:85983558-85983577 | None:intergenic | 35.0% | |
| TTTACACGTTGTTGGAGATT+TGG | - | chr6_4:85983557-85983576 | Msa1001880:CDS | 35.0% | |
| TATATGAGTGTGGCATCATA+CGG | - | chr6_4:85983813-85983832 | Msa1001880:intron | 35.0% | |
| ! | GAGAATGGAGACATTTTTGT+AGG | - | chr6_4:85983873-85983892 | Msa1001880:intron | 35.0% |
| TGAAACCAAGATGTACATTG+AGG | + | chr6_4:85983963-85983982 | None:intergenic | 35.0% | |
| AGATGTTAATTATGCCCACG+AGG | - | chr6_4:85983429-85983448 | Msa1001880:three_prime_UTR | 40.0% | |
| AGCCATCAATAAACTGAGGT+AGG | + | chr6_4:85983482-85983501 | None:intergenic | 40.0% | |
| GCATAGCCATCAATAAACTG+AGG | + | chr6_4:85983486-85983505 | None:intergenic | 40.0% | |
| GATGATCCTTTACACGTTGT+TGG | - | chr6_4:85983549-85983568 | Msa1001880:CDS | 40.0% | |
| ! | ATTCTCTGTCACTTGCAATG+CGG | + | chr6_4:85983604-85983623 | None:intergenic | 40.0% |
| GCAAGTGACAGAGAATAAGA+TGG | - | chr6_4:85983608-85983627 | Msa1001880:CDS | 40.0% | |
| CTTGCTGCACAAACTTTGTT+GGG | + | chr6_4:85983739-85983758 | None:intergenic | 40.0% | |
| TCTGCTAGAGAATAAGCCAA+AGG | - | chr6_4:85983760-85983779 | Msa1001880:CDS | 40.0% | |
| AAAATGGCCTCAAAGTTGTC+AGG | - | chr6_4:85983897-85983916 | Msa1001880:intron | 40.0% | |
| AAGGACCTCAATGTACATCT+TGG | - | chr6_4:85983955-85983974 | Msa1001880:CDS | 40.0% | |
| CACAGCAAAAGGGGAAAAAA+CGG | + | chr6_4:85984084-85984103 | None:intergenic | 40.0% | |
| ! | ATTGATGGCTATGCCCAAGA+TGG | - | chr6_4:85983492-85983511 | Msa1001880:three_prime_UTR | 45.0% |
| AAGGATCATCCTTCAGACCA+GGG | + | chr6_4:85983539-85983558 | None:intergenic | 45.0% | |
| AAAGGATCATCCTTCAGACC+AGG | + | chr6_4:85983540-85983559 | None:intergenic | 45.0% | |
| ! | GCTTGCTGCACAAACTTTGT+TGG | + | chr6_4:85983740-85983759 | None:intergenic | 45.0% |
| TAAGCCAAAGGATGTTGCTC+TGG | - | chr6_4:85983772-85983791 | Msa1001880:CDS | 45.0% | |
| ATGACCAGAGCAACATCCTT+TGG | + | chr6_4:85983779-85983798 | None:intergenic | 45.0% | |
| ATGTTGCTCTGGTCATCGAA+TGG | - | chr6_4:85983783-85983802 | Msa1001880:CDS | 45.0% | |
| TGTTGCTCTGGTCATCGAAT+GGG | - | chr6_4:85983784-85983803 | Msa1001880:CDS | 45.0% | |
| TGGGGGAATGTATATGAGTG+TGG | - | chr6_4:85983803-85983822 | Msa1001880:intron | 45.0% | |
| TCTCCATTCTCGTACCAAGT+AGG | + | chr6_4:85983864-85983883 | None:intergenic | 45.0% | |
| AAAGTTGTCAGGACTCAGGA+GGG | - | chr6_4:85983908-85983927 | Msa1001880:CDS | 45.0% | |
| AAGGTCAACGGCACCATCTT+GGG | + | chr6_4:85983508-85983527 | None:intergenic | 50.0% | |
| ! | AGGGAGTGCATCAAGGTCAA+CGG | + | chr6_4:85983520-85983539 | None:intergenic | 50.0% |
| GTTGCTCTGGTCATCGAATG+GGG | - | chr6_4:85983785-85983804 | Msa1001880:intron | 50.0% | |
| TTGCTCTGGTCATCGAATGG+GGG | - | chr6_4:85983786-85983805 | Msa1001880:intron | 50.0% | |
| TCTCGTACCAAGTAGGTGCA+GGG | + | chr6_4:85983857-85983876 | None:intergenic | 50.0% | |
| TTCTCGTACCAAGTAGGTGC+AGG | + | chr6_4:85983858-85983877 | None:intergenic | 50.0% | |
| GCACCTACTTGGTACGAGAA+TGG | - | chr6_4:85983858-85983877 | Msa1001880:intron | 50.0% | |
| CCTGAGTCCTGACAACTTTG+AGG | + | chr6_4:85983907-85983926 | None:intergenic | 50.0% | |
| CCTCAAAGTTGTCAGGACTC+AGG | - | chr6_4:85983904-85983923 | Msa1001880:intron | 50.0% | |
| CAAAGTTGTCAGGACTCAGG+AGG | - | chr6_4:85983907-85983926 | Msa1001880:CDS | 50.0% | |
| ACTCAGGAGGGTAAAGCTGA+CGG | - | chr6_4:85983920-85983939 | Msa1001880:CDS | 50.0% | |
| CTGACGGCATATCTTGCGAA+AGG | - | chr6_4:85983936-85983955 | Msa1001880:CDS | 50.0% | |
| CAAGGTCAACGGCACCATCT+TGG | + | chr6_4:85983509-85983528 | None:intergenic | 55.0% | |
| GTTGACCTTGATGCACTCCC+TGG | - | chr6_4:85983519-85983538 | Msa1001880:exon | 55.0% | |
| ! | TCAGACCAGGGAGTGCATCA+AGG | + | chr6_4:85983527-85983546 | None:intergenic | 55.0% |
| TGATGCACTCCCTGGTCTGA+AGG | - | chr6_4:85983527-85983546 | Msa1001880:exon | 55.0% | |
| CAAGTAGGTGCAGGGAGTGA+GGG | + | chr6_4:85983849-85983868 | None:intergenic | 55.0% | |
| !! | ATGCCCACGAGGTGCTGATC+TGG | - | chr6_4:85983440-85983459 | Msa1001880:three_prime_UTR | 60.0% |
| GTGCAGGGAGTGAGGGTTTG+AGG | + | chr6_4:85983842-85983861 | None:intergenic | 60.0% | |
| CCAAGTAGGTGCAGGGAGTG+AGG | + | chr6_4:85983850-85983869 | None:intergenic | 60.0% | |
| CCTCACTCCCTGCACCTACT+TGG | - | chr6_4:85983847-85983866 | Msa1001880:intron | 60.0% | |
| CGGCCAGATCAGCACCTCGT+GGG | + | chr6_4:85983446-85983465 | None:intergenic | 65.0% | |
| GCGGCCAGATCAGCACCTCG+TGG | + | chr6_4:85983447-85983466 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 85983424 | 85984110 | 85983424 | ID=Msa1001880;Name=Msa1001880 |
| chr6_4 | mRNA | 85983424 | 85984110 | 85983424 | ID=Msa1001880-mRNA-1;Parent=Msa1001880;Name=Msa1001880-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0.5|0.33|0.66|0.5|0.66|3|113|138 |
| chr6_4 | exon | 85983424 | 85983803 | 85983424 | ID=Msa1001880-mRNA-1:exon:16958;Parent=Msa1001880-mRNA-1 |
| chr6_4 | exon | 85983905 | 85984045 | 85983905 | ID=Msa1001880-mRNA-1:exon:16957;Parent=Msa1001880-mRNA-1 |
| chr6_4 | exon | 85984102 | 85984110 | 85984102 | ID=Msa1001880-mRNA-1:exon:16956;Parent=Msa1001880-mRNA-1 |
| chr6_4 | CDS | 85984102 | 85984110 | 85984102 | ID=Msa1001880-mRNA-1:cds;Parent=Msa1001880-mRNA-1 |
| chr6_4 | CDS | 85983905 | 85984045 | 85983905 | ID=Msa1001880-mRNA-1:cds;Parent=Msa1001880-mRNA-1 |
| chr6_4 | CDS | 85983537 | 85983803 | 85983537 | ID=Msa1001880-mRNA-1:cds;Parent=Msa1001880-mRNA-1 |
| chr6_4 | three_prime_UTR | 85983424 | 85983536 | 85983424 | ID=Msa1001880-mRNA-1:three_prime_utr;Parent=Msa1001880-mRNA-1 |
| Gene Sequence |
| Protein sequence |
