AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1456730


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456730 MtrunA17_Chr2g0318801 95.614 114 5 0 40 153 1 114 1.14e-75 221
Msa1456730 MtrunA17_Chr2g0318791 88.596 114 13 0 40 153 1 114 2.95e-67 199
Msa1456730 MtrunA17_Chr2g0318761 85.321 109 16 0 45 153 2 110 1.10e-64 193
Msa1456730 MtrunA17_Chr2g0280881 63.830 94 33 1 48 140 14 107 3.72e-35 118
Msa1456730 MtrunA17_Chr2g0318861 60.417 96 37 1 48 142 10 105 4.56e-34 115
Msa1456730 MtrunA17_Chr8g0361421 61.458 96 36 1 48 142 11 106 4.60e-34 115
Msa1456730 MtrunA17_Chr2g0323211 56.122 98 42 1 41 137 3 100 5.93e-32 110
Msa1456730 MtrunA17_Chr4g0023231 59.341 91 36 1 48 137 6 96 3.11e-30 105
Msa1456730 MtrunA17_Chr8g0346481 53.191 94 43 1 45 137 16 109 3.52e-27 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456730 AT5G54490.1 56.842 95 39 2 48 140 19 113 1.71e-31 109
Msa1456730 AT4G27280.1 55.208 96 41 2 47 140 18 113 4.32e-29 103
Msa1456730 AT2G46600.1 44.828 116 58 2 23 137 2 112 1.39e-25 95.1

Find 33 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACGGGTTTCATTTGCTTA+TGG 0.204248 tig0037710:-6103 Msa1456730:CDS
CCATCCATGGTTGCGAGTTT+AGG 0.278334 tig0037710:-6153 Msa1456730:CDS
TTAGGAGCAGAAGCATTTAT+AGG 0.308592 tig0037710:-6135 Msa1456730:CDS
TGATGTTCCTAAGATGAAAA+TGG 0.314622 tig0037710:-6580 Msa1456730:CDS
CATGCTAATGGAAGGTGATT+TGG 0.335601 tig0037710:-5992 Msa1456730:CDS
AAAATATGCACAAATCTTCA+AGG 0.428589 tig0037710:-5892 Msa1456730:CDS
TAGGTTGAGTCCATGTTTAA+TGG 0.441618 tig0037710:-5923 Msa1456730:CDS
AAATGGTAATAATTGATTGT+AGG 0.474579 tig0037710:-6563 Msa1456730:CDS
CCTAAACTCGCAACCATGGA+TGG 0.475656 tig0037710:+6153 Msa1456730:intergenic
CACAAGTTTCCACATGGAGT+TGG 0.491047 tig0037710:-6205 Msa1456730:CDS
AAGATGAATTGTTTCATGCT+TGG 0.497368 tig0037710:-6045 Msa1456730:CDS
TGAGGACTACTTTCCATCCA+TGG 0.498192 tig0037710:-6166 Msa1456730:CDS
TGAGCTCGTGTACATGCTAA+TGG 0.499730 tig0037710:-6004 Msa1456730:CDS
ATTGATTGTAGGAGTAATGA+AGG 0.507954 tig0037710:-6552 Msa1456730:CDS
ATGAGAATGCAAAACTCCAT+TGG 0.509723 tig0037710:+5949 Msa1456730:intergenic
GAATTGTTTCATGCTTGGTA+TGG 0.522428 tig0037710:-6040 Msa1456730:CDS
TTGAGTCCATGTTTAATGGA+TGG 0.531601 tig0037710:-5919 Msa1456730:CDS
TTTATAGGTGAACTTTGCTA+CGG 0.531654 tig0037710:-6120 Msa1456730:CDS
TTATAGGTGAACTTTGCTAC+GGG 0.538698 tig0037710:-6119 Msa1456730:CDS
TTTGCTTATGGATGTCAACA+AGG 0.557910 tig0037710:-6091 Msa1456730:CDS
TCATGCTTGGTATGGAGGTG+AGG 0.562351 tig0037710:-6032 Msa1456730:CDS
CATGCTTGGTATGGAGGTGA+GGG 0.564464 tig0037710:-6031 Msa1456730:CDS
AGATGGTGCTTTAAATCCAA+TGG 0.565219 tig0037710:-5965 Msa1456730:CDS
TTGTTTCATGCTTGGTATGG+AGG 0.565973 tig0037710:-6037 Msa1456730:CDS
TGCTCCTAAACTCGCAACCA+TGG 0.581169 tig0037710:+6149 Msa1456730:intergenic
GAGTGTCATCACATTAACAT+TGG 0.590328 tig0037710:+5862 Msa1456730:intergenic
TTCACTCACAAGTTTCCACA+TGG 0.601660 tig0037710:-6211 Msa1456730:CDS
TTAGGTCCATCCATTAAACA+TGG 0.608146 tig0037710:+5913 Msa1456730:intergenic
GAAGGTGATTTGGATGGAGA+TGG 0.627275 tig0037710:-5982 Msa1456730:CDS
TATCAAAATCCAACTCCATG+TGG 0.653461 tig0037710:+6196 Msa1456730:intergenic
TTGCTTATGGATGTCAACAA+GGG 0.661501 tig0037710:-6090 Msa1456730:CDS
CTAATGGAAGGTGATTTGGA+TGG 0.674005 tig0037710:-5988 Msa1456730:CDS
CTCGTGTACATGCTAATGGA+AGG 0.692064 tig0037710:-6000 Msa1456730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTACCATTTTCATCTT+AGG + tig0037710:5900-5919 Msa1456730:intergenic 20.0%
!! AAATGGTAATAATTGATTGT+AGG - tig0037710:5907-5926 Msa1456730:CDS 20.0%
!!! AAATCTAATTTTTCTCTGAT+AGG - tig0037710:6067-6086 Msa1456730:CDS 20.0%
!!! AATCTAATTTTTCTCTGATA+GGG - tig0037710:6068-6087 Msa1456730:CDS 20.0%
!!! ATTATTACCATTTTCATCTT+AGG + tig0037710:5900-5919 Msa1456730:intergenic 20.0%
!! AAATGGTAATAATTGATTGT+AGG - tig0037710:5907-5926 Msa1456730:CDS 20.0%
!!! AAATCTAATTTTTCTCTGAT+AGG - tig0037710:6067-6086 Msa1456730:CDS 20.0%
!!! AATCTAATTTTTCTCTGATA+GGG - tig0037710:6068-6087 Msa1456730:CDS 20.0%
!! ATCTAATTTTTCTCTGATAG+GGG - tig0037710:6069-6088 Msa1456730:CDS 25.0%
!! TGAAGATTTGTGCATATTTT+AGG + tig0037710:6578-6597 Msa1456730:intergenic 25.0%
! AAAATATGCACAAATCTTCA+AGG - tig0037710:6578-6597 Msa1456730:CDS 25.0%
!! ATCTAATTTTTCTCTGATAG+GGG - tig0037710:6069-6088 Msa1456730:CDS 25.0%
!! TGAAGATTTGTGCATATTTT+AGG + tig0037710:6578-6597 Msa1456730:intergenic 25.0%
! AAAATATGCACAAATCTTCA+AGG - tig0037710:6578-6597 Msa1456730:CDS 25.0%
TGATGTTCCTAAGATGAAAA+TGG - tig0037710:5890-5909 Msa1456730:CDS 30.0%
ATTGATTGTAGGAGTAATGA+AGG - tig0037710:5918-5937 Msa1456730:CDS 30.0%
TGATGTTCCTAAGATGAAAA+TGG - tig0037710:5890-5909 Msa1456730:CDS 30.0%
TGATGTTCCTAAGATGAAAA+TGG - tig0037710:5890-5909 Msa1456730:CDS 30.0%
! TCAGTTACCATTTTCATCTT+AGG + tig0037710:6026-6045 Msa1456730:intergenic 30.0%
!! ATTATTAGCTTGTGCTAGAA+GGG + tig0037710:6197-6216 Msa1456730:intergenic 30.0%
!! AATTATTAGCTTGTGCTAGA+AGG + tig0037710:6198-6217 Msa1456730:intergenic 30.0%
!! GGATTTTGATATGGATTTTG+AGG - tig0037710:6286-6305 Msa1456730:intron 30.0%
TTTATAGGTGAACTTTGCTA+CGG - tig0037710:6350-6369 Msa1456730:intron 30.0%
AAGATGAATTGTTTCATGCT+TGG - tig0037710:6425-6444 Msa1456730:intron 30.0%
! AGTTTTGCATTCTCATGTTT+AGG - tig0037710:6528-6547 Msa1456730:intron 30.0%
TGATGTTCCTAAGATGAAAA+TGG - tig0037710:5890-5909 Msa1456730:CDS 30.0%
ATTGATTGTAGGAGTAATGA+AGG - tig0037710:5918-5937 Msa1456730:CDS 30.0%
TGATGTTCCTAAGATGAAAA+TGG - tig0037710:5890-5909 Msa1456730:CDS 30.0%
TGATGTTCCTAAGATGAAAA+TGG - tig0037710:5890-5909 Msa1456730:CDS 30.0%
! TCAGTTACCATTTTCATCTT+AGG + tig0037710:6026-6045 Msa1456730:intergenic 30.0%
!! ATTATTAGCTTGTGCTAGAA+GGG + tig0037710:6197-6216 Msa1456730:intergenic 30.0%
!! AATTATTAGCTTGTGCTAGA+AGG + tig0037710:6198-6217 Msa1456730:intergenic 30.0%
!! GGATTTTGATATGGATTTTG+AGG - tig0037710:6286-6305 Msa1456730:intron 30.0%
TTTATAGGTGAACTTTGCTA+CGG - tig0037710:6350-6369 Msa1456730:intron 30.0%
AAGATGAATTGTTTCATGCT+TGG - tig0037710:6425-6444 Msa1456730:intron 30.0%
! AGTTTTGCATTCTCATGTTT+AGG - tig0037710:6528-6547 Msa1456730:intron 30.0%
! TCAGCTACCATTTTCATCTT+AGG + tig0037710:5964-5983 Msa1456730:intergenic 35.0%
TGAAAATGGTAGCTGATTGT+AGG - tig0037710:5968-5987 Msa1456730:CDS 35.0%
CTGATTGTAGGAGTAAATGA+AGG - tig0037710:5980-5999 Msa1456730:CDS 35.0%
ATATTACCCTCAATTCGTCA+AGG - tig0037710:6093-6112 Msa1456730:CDS 35.0%
TATTACCCTCAATTCGTCAA+GGG - tig0037710:6094-6113 Msa1456730:CDS 35.0%
ATTATCCCTTGACGAATTGA+GGG + tig0037710:6102-6121 Msa1456730:intergenic 35.0%
TTGCTAGGAACAATGTGAAA+GGG + tig0037710:6225-6244 Msa1456730:intergenic 35.0%
AACTTCAAGTAGAAGTTGCT+AGG + tig0037710:6240-6259 Msa1456730:intergenic 35.0%
TATCAAAATCCAACTCCATG+TGG + tig0037710:6277-6296 Msa1456730:intergenic 35.0%
!! CATGGAGTTGGATTTTGATA+TGG - tig0037710:6277-6296 Msa1456730:intron 35.0%
!! TTAGGAGCAGAAGCATTTAT+AGG - tig0037710:6335-6354 Msa1456730:intron 35.0%
TTATAGGTGAACTTTGCTAC+GGG - tig0037710:6351-6370 Msa1456730:intron 35.0%
TTTGCTTATGGATGTCAACA+AGG - tig0037710:6379-6398 Msa1456730:intron 35.0%
TTGCTTATGGATGTCAACAA+GGG - tig0037710:6380-6399 Msa1456730:intron 35.0%
GAATTGTTTCATGCTTGGTA+TGG - tig0037710:6430-6449 Msa1456730:intron 35.0%
! AGATGGTGCTTTAAATCCAA+TGG - tig0037710:6505-6524 Msa1456730:intron 35.0%
ATGAGAATGCAAAACTCCAT+TGG + tig0037710:6524-6543 Msa1456730:intergenic 35.0%
! TAGGTTGAGTCCATGTTTAA+TGG - tig0037710:6547-6566 Msa1456730:CDS 35.0%
TTGAGTCCATGTTTAATGGA+TGG - tig0037710:6551-6570 Msa1456730:CDS 35.0%
TTAGGTCCATCCATTAAACA+TGG + tig0037710:6560-6579 Msa1456730:intergenic 35.0%
! TCAGCTACCATTTTCATCTT+AGG + tig0037710:5964-5983 Msa1456730:intergenic 35.0%
TGAAAATGGTAGCTGATTGT+AGG - tig0037710:5968-5987 Msa1456730:CDS 35.0%
CTGATTGTAGGAGTAAATGA+AGG - tig0037710:5980-5999 Msa1456730:CDS 35.0%
ATATTACCCTCAATTCGTCA+AGG - tig0037710:6093-6112 Msa1456730:CDS 35.0%
TATTACCCTCAATTCGTCAA+GGG - tig0037710:6094-6113 Msa1456730:CDS 35.0%
ATTATCCCTTGACGAATTGA+GGG + tig0037710:6102-6121 Msa1456730:intergenic 35.0%
TTGCTAGGAACAATGTGAAA+GGG + tig0037710:6225-6244 Msa1456730:intergenic 35.0%
AACTTCAAGTAGAAGTTGCT+AGG + tig0037710:6240-6259 Msa1456730:intergenic 35.0%
TATCAAAATCCAACTCCATG+TGG + tig0037710:6277-6296 Msa1456730:intergenic 35.0%
!! CATGGAGTTGGATTTTGATA+TGG - tig0037710:6277-6296 Msa1456730:intron 35.0%
!! TTAGGAGCAGAAGCATTTAT+AGG - tig0037710:6335-6354 Msa1456730:intron 35.0%
TTATAGGTGAACTTTGCTAC+GGG - tig0037710:6351-6370 Msa1456730:intron 35.0%
TTTGCTTATGGATGTCAACA+AGG - tig0037710:6379-6398 Msa1456730:intron 35.0%
TTGCTTATGGATGTCAACAA+GGG - tig0037710:6380-6399 Msa1456730:intron 35.0%
GAATTGTTTCATGCTTGGTA+TGG - tig0037710:6430-6449 Msa1456730:intron 35.0%
! AGATGGTGCTTTAAATCCAA+TGG - tig0037710:6505-6524 Msa1456730:intron 35.0%
ATGAGAATGCAAAACTCCAT+TGG + tig0037710:6524-6543 Msa1456730:intergenic 35.0%
! TAGGTTGAGTCCATGTTTAA+TGG - tig0037710:6547-6566 Msa1456730:CDS 35.0%
TTGAGTCCATGTTTAATGGA+TGG - tig0037710:6551-6570 Msa1456730:CDS 35.0%
TTAGGTCCATCCATTAAACA+TGG + tig0037710:6560-6579 Msa1456730:intergenic 35.0%
CATTATCCCTTGACGAATTG+AGG + tig0037710:6103-6122 Msa1456730:intergenic 40.0%
TGCTAGGAACAATGTGAAAG+GGG + tig0037710:6224-6243 Msa1456730:intergenic 40.0%
GTTGCTAGGAACAATGTGAA+AGG + tig0037710:6226-6245 Msa1456730:intergenic 40.0%
TTCACTCACAAGTTTCCACA+TGG - tig0037710:6259-6278 Msa1456730:intron 40.0%
CTACGGGTTTCATTTGCTTA+TGG - tig0037710:6367-6386 Msa1456730:intron 40.0%
TTGTTTCATGCTTGGTATGG+AGG - tig0037710:6433-6452 Msa1456730:intron 40.0%
CATGCTAATGGAAGGTGATT+TGG - tig0037710:6478-6497 Msa1456730:intron 40.0%
! CTAATGGAAGGTGATTTGGA+TGG - tig0037710:6482-6501 Msa1456730:intron 40.0%
CATTATCCCTTGACGAATTG+AGG + tig0037710:6103-6122 Msa1456730:intergenic 40.0%
TGCTAGGAACAATGTGAAAG+GGG + tig0037710:6224-6243 Msa1456730:intergenic 40.0%
GTTGCTAGGAACAATGTGAA+AGG + tig0037710:6226-6245 Msa1456730:intergenic 40.0%
TTCACTCACAAGTTTCCACA+TGG - tig0037710:6259-6278 Msa1456730:intron 40.0%
CTACGGGTTTCATTTGCTTA+TGG - tig0037710:6367-6386 Msa1456730:intron 40.0%
TTGTTTCATGCTTGGTATGG+AGG - tig0037710:6433-6452 Msa1456730:intron 40.0%
CATGCTAATGGAAGGTGATT+TGG - tig0037710:6478-6497 Msa1456730:intron 40.0%
! CTAATGGAAGGTGATTTGGA+TGG - tig0037710:6482-6501 Msa1456730:intron 40.0%
CACAAGTTTCCACATGGAGT+TGG - tig0037710:6265-6284 Msa1456730:intron 45.0%
TGAGGACTACTTTCCATCCA+TGG - tig0037710:6304-6323 Msa1456730:intron 45.0%
TGAGCTCGTGTACATGCTAA+TGG - tig0037710:6466-6485 Msa1456730:intron 45.0%
CTCGTGTACATGCTAATGGA+AGG - tig0037710:6470-6489 Msa1456730:intron 45.0%
! GAAGGTGATTTGGATGGAGA+TGG - tig0037710:6488-6507 Msa1456730:intron 45.0%
CACAAGTTTCCACATGGAGT+TGG - tig0037710:6265-6284 Msa1456730:intron 45.0%
TGAGGACTACTTTCCATCCA+TGG - tig0037710:6304-6323 Msa1456730:intron 45.0%
TGAGCTCGTGTACATGCTAA+TGG - tig0037710:6466-6485 Msa1456730:intron 45.0%
CTCGTGTACATGCTAATGGA+AGG - tig0037710:6470-6489 Msa1456730:intron 45.0%
! GAAGGTGATTTGGATGGAGA+TGG - tig0037710:6488-6507 Msa1456730:intron 45.0%
CCTAAACTCGCAACCATGGA+TGG + tig0037710:6320-6339 Msa1456730:intergenic 50.0%
CCATCCATGGTTGCGAGTTT+AGG - tig0037710:6317-6336 Msa1456730:intron 50.0%
TGCTCCTAAACTCGCAACCA+TGG + tig0037710:6324-6343 Msa1456730:intergenic 50.0%
TCATGCTTGGTATGGAGGTG+AGG - tig0037710:6438-6457 Msa1456730:intron 50.0%
CATGCTTGGTATGGAGGTGA+GGG - tig0037710:6439-6458 Msa1456730:intron 50.0%
CCTAAACTCGCAACCATGGA+TGG + tig0037710:6320-6339 Msa1456730:intergenic 50.0%
CCATCCATGGTTGCGAGTTT+AGG - tig0037710:6317-6336 Msa1456730:intron 50.0%
TGCTCCTAAACTCGCAACCA+TGG + tig0037710:6324-6343 Msa1456730:intergenic 50.0%
TCATGCTTGGTATGGAGGTG+AGG - tig0037710:6438-6457 Msa1456730:intron 50.0%
CATGCTTGGTATGGAGGTGA+GGG - tig0037710:6439-6458 Msa1456730:intron 50.0%
Chromosome Type Strat End Strand Name
tig0037710 gene 5870 6622 5870 ID=Msa1456730;Name=Msa1456730
tig0037710 mRNA 5870 6622 5870 ID=Msa1456730-mRNA-1;Parent=Msa1456730;Name=Msa1456730-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|0.5|1|1|2|0|153
tig0037710 exon 6542 6622 6542 ID=Msa1456730-mRNA-1:exon:20864;Parent=Msa1456730-mRNA-1
tig0037710 exon 5870 6250 5870 ID=Msa1456730-mRNA-1:exon:20863;Parent=Msa1456730-mRNA-1
tig0037710 CDS 6542 6622 6542 ID=Msa1456730-mRNA-1:cds;Parent=Msa1456730-mRNA-1
tig0037710 CDS 5870 6250 5870 ID=Msa1456730-mRNA-1:cds;Parent=Msa1456730-mRNA-1
Gene Sequence

>Msa1456730

ATGAAGGAGATGAAGAAGTTTGATGTTCCTAAGATGAAAATGGTAATAATTGATTGTAGGAGTAATGAAGGAGATGAAGAACAACTTCTACTTGAAGTTTCACTCACAAGTTTCCACATGGAGTTGGATTTTGATATGGATTTTGAGGACTACTTTCCATCCATGGTTGCGAGTTTAGGAGCAGAAGCATTTATAGGTGAACTTTGCTACGGGTTTCATTTGCTTATGGATGTCAACAAGGGTCTTATAACATTTGAGAGTTTGAAGATGAATTGTTTCATGCTTGGTATGGAGGTGAGGGATGATGAGCTCGTGTACATGCTAATGGAAGGTGATTTGGATGGAGATGGTGCTTTAAATCCAATGGAGTTTTGCATTCTCATGTTTAGGTTGAGTCCATGTTTAATGGATGGACCTAAAATATGCACAAATCTTCAAGGTGTGAATCCAATGTTAATGTGA

Protein sequence

>Msa1456730

MKEMKKFDVPKMKMVIIDCRSNEGDEEQLLLEVSLTSFHMELDFDMDFEDYFPSMVASLGAEAFIGELCYGFHLLMDVNKGLITFESLKMNCFMLGMEVRDDELVYMLMEGDLDGDGALNPMEFCILMFRLSPCLMDGPKICTNLQGVNPMLM*