AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1003170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003170 MtrunA17_Chr7g0214211 95.954 173 7 0 1 173 1 173 3.96e-113 318
Msa1003170 MtrunA17_Chr7g0224361 46.309 149 71 5 26 173 33 173 1.51e-41 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1003170 AT5G13140.1 50.667 150 66 5 26 173 35 178 5.75e-44 146
Msa1003170 AT5G41050.1 46.939 147 72 4 26 171 29 170 1.18e-37 127
Msa1003170 AT3G26960.1 43.478 138 69 3 26 161 33 163 4.39e-32 113

Find 73 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAATGAAGAAAGGATTT+TGG 0.210001 7_1:+226182 None:intergenic
TGATAGATGTAATTCATAAA+AGG 0.239602 7_1:+226073 None:intergenic
TGGACCCCTTTCATTAAGTT+TGG 0.252347 7_1:-226338 Msa1003170:three_prime_UTR
AGTGGCGTGCATCGGACTTT+TGG 0.261878 7_1:+226560 None:intergenic
TATGCTCAAATTAATTCATT+TGG 0.298493 7_1:-226596 Msa1003170:exon
ATTGGATCCATGTGGAATAT+TGG 0.305436 7_1:+227403 None:intergenic
AGGTGCTATAGAATGAATTT+TGG 0.319346 7_1:+226374 None:intergenic
ATTGTCCGCTTTAGATATTA+TGG 0.324237 7_1:-226527 Msa1003170:three_prime_UTR
TTTATCTGTTCGTTGGTTCT+TGG 0.331613 7_1:+226900 None:intergenic
GGACCCCTTTCATTAAGTTT+GGG 0.332848 7_1:-226337 Msa1003170:three_prime_UTR
CCTTTCATTAAGTTTGGGAT+TGG 0.340938 7_1:-226332 Msa1003170:three_prime_UTR
GTGGGGTCCCCTAAAATAAA+AGG 0.343139 7_1:+226252 None:intergenic
GGACATATCTATAAACAAAA+TGG 0.345515 7_1:+225981 None:intergenic
AATGAATTAATTTGAGCATA+AGG 0.365031 7_1:+226599 None:intergenic
GGATAAGGATATTGAGTAAG+AGG 0.392047 7_1:+226295 None:intergenic
TATTGTGATGCCTGCTCAAC+CGG 0.409183 7_1:-227283 Msa1003170:CDS
TATTGAGTAAGAGGAAGAGA+AGG 0.415307 7_1:+226304 None:intergenic
CTTTCATTAAGTTTGGGATT+GGG 0.420070 7_1:-226331 Msa1003170:three_prime_UTR
CTCTGCGAAGCAAGCTTGAT+AGG 0.425118 7_1:-226765 Msa1003170:CDS
CTCAACACTGATAGTGGTTG+TGG 0.425792 7_1:+227315 None:intergenic
ATTGAGAGTGGTGTAGGATA+AGG 0.427502 7_1:+226280 None:intergenic
GATTGAGCAATGAGAACAAA+TGG 0.438625 7_1:+227340 None:intergenic
AGAGAAAGTGCCGGTTGAGC+AGG 0.454772 7_1:+227273 None:intergenic
AGAAGAAGGAAGAAACCAAT+TGG 0.454824 7_1:+227385 None:intergenic
GCTGCTAGCTAGCTAGCTAT+AGG 0.455157 7_1:+226113 None:intergenic
GAGGTGATTGAGAGTGGTGT+AGG 0.457528 7_1:+226274 None:intergenic
AATTCCCTCAACACTGATAG+TGG 0.459627 7_1:+227309 None:intergenic
GATAGATGTAATTCATAAAA+GGG 0.468871 7_1:+226074 None:intergenic
AACCTATGTGTATACAGCTT+GGG 0.470069 7_1:-226663 Msa1003170:CDS
ATATTCCACATGGATCCAAT+TGG 0.475963 7_1:-227400 Msa1003170:exon
CAATCCCAAACTTAATGAAA+GGG 0.477690 7_1:+226333 None:intergenic
TGAGAAATTTATCTGTTCGT+TGG 0.493906 7_1:+226893 None:intergenic
CTTCCTGTGCTCTTCAAGAA+AGG 0.504211 7_1:+226711 None:intergenic
CACACAACAATATATACATA+TGG 0.507745 7_1:-227456 Msa1003170:five_prime_UTR
GTTCCTTTCTTGAAGAGCAC+AGG 0.511194 7_1:-226714 Msa1003170:CDS
TAACCTATGTGTATACAGCT+TGG 0.520473 7_1:-226664 Msa1003170:CDS
ATTGAGTAAGAGGAAGAGAA+GGG 0.521172 7_1:+226305 None:intergenic
AACTTAATGAAAGGGGTCCA+AGG 0.523943 7_1:+226341 None:intergenic
ACAACCACTATCAGTGTTGA+GGG 0.524635 7_1:-227313 Msa1003170:CDS
CCAATCCCAAACTTAATGAA+AGG 0.528525 7_1:+226332 None:intergenic
AAACACAGTTATCTCTTGCC+AGG 0.531682 7_1:-227250 Msa1003170:intron
AAAGTTGTTGTTGAATTGAG+TGG 0.538831 7_1:-226477 Msa1003170:three_prime_UTR
ACAAGAGGAATCCAAGTTGT+GGG 0.540830 7_1:+226234 None:intergenic
CTTAGAAAAGTGGCGTGCAT+CGG 0.557367 7_1:+226552 None:intergenic
CAAATTAAATATACCATCAG+TGG 0.559042 7_1:-226838 Msa1003170:CDS
CAGTTAGTTCCATCCACTGA+TGG 0.559252 7_1:+226825 None:intergenic
CGGACAATACCTTAGAAAAG+TGG 0.567729 7_1:+226542 None:intergenic
CACAAGAGGAATCCAAGTTG+TGG 0.568323 7_1:+226233 None:intergenic
CACAACCACTATCAGTGTTG+AGG 0.573227 7_1:-227314 Msa1003170:CDS
CTTGATTCCAATATTCCACA+TGG 0.573944 7_1:-227410 Msa1003170:exon
TCAACACTGATAGTGGTTGT+GGG 0.576625 7_1:+227316 None:intergenic
AATCTGTGAATTACCATCCA+TGG 0.577346 7_1:+226794 None:intergenic
GGGTCCAAGGAAAGAAAAGA+AGG 0.578723 7_1:+226354 None:intergenic
ACAGCAGAGATGTCAGAAGA+AGG 0.579115 7_1:+227371 None:intergenic
AACTGTGAAATTGCCATGGA+TGG 0.583388 7_1:-226807 Msa1003170:CDS
ATATATTGTTGTGTGACAGT+TGG 0.583761 7_1:+227464 None:intergenic
TAAAAGGAGGTGATTGAGAG+TGG 0.589362 7_1:+226268 None:intergenic
TTAAATATACCATCAGTGGA+TGG 0.597816 7_1:-226834 Msa1003170:CDS
AACTAACTGTGAAATTGCCA+TGG 0.599482 7_1:-226811 Msa1003170:CDS
AAGTTGTTGTTGAATTGAGT+GGG 0.604852 7_1:-226476 Msa1003170:three_prime_UTR
CTCACATTACTTGAAAATCG+AGG 0.632356 7_1:+226425 None:intergenic
AGTTTCCATAATATCTAAAG+CGG 0.633242 7_1:+226522 None:intergenic
GCAAGAATAAGAAGATGATG+AGG 0.636237 7_1:+226740 None:intergenic
GAATAGAACAACAGACAGAG+AGG 0.636780 7_1:-226868 Msa1003170:CDS
CTCCCAAGCTGTATACACAT+AGG 0.650090 7_1:+226661 None:intergenic
TGTGACAGTTGGAGTGTGTG+CGG 0.650489 7_1:+227475 None:intergenic
GTCGAGAGAGAACACACAAG+AGG 0.653695 7_1:+226219 None:intergenic
AATCCCAAACTTAATGAAAG+GGG 0.655786 7_1:+226334 None:intergenic
AATAGAACAACAGACAGAGA+GGG 0.660031 7_1:-226867 Msa1003170:CDS
ACTGTGTTTAGAGAAAGTGC+CGG 0.671971 7_1:+227264 None:intergenic
GGGTCCCCTAAAATAAAAGG+AGG 0.676821 7_1:+226255 None:intergenic
CAAGAGGAATCCAAGTTGTG+GGG 0.691066 7_1:+226235 None:intergenic
AGAAACCAATTGGATCCATG+TGG 0.695775 7_1:+227395 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCATTTAATTATACATATAA+TGG + chr7_1:226277-226296 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG + chr7_1:227288-227307 None:intergenic 15.0%
!! GCATTTAATTATACATATAA+TGG + chr7_1:226277-226296 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG + chr7_1:227288-227307 None:intergenic 15.0%
!! TATCTATGATGATATATATC+AGG - chr7_1:226497-226516 Msa1003170:three_prime_UTR 20.0%
!!! AATGAATTAATTTGAGCATA+AGG + chr7_1:226864-226883 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG - chr7_1:226864-226883 Msa1003170:CDS 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG + chr7_1:227074-227093 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG + chr7_1:227389-227408 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG + chr7_1:227390-227409 None:intergenic 20.0%
!! TATCTATGATGATATATATC+AGG - chr7_1:226497-226516 Msa1003170:three_prime_UTR 20.0%
!!! AATGAATTAATTTGAGCATA+AGG + chr7_1:226864-226883 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG - chr7_1:226864-226883 Msa1003170:CDS 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG + chr7_1:227074-227093 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG + chr7_1:227389-227408 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG + chr7_1:227390-227409 None:intergenic 20.0%
! CACACAACAATATATACATA+TGG - chr7_1:226004-226023 Msa1003170:three_prime_UTR 25.0%
! CAAATTAAATATACCATCAG+TGG - chr7_1:226622-226641 Msa1003170:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG + chr7_1:226842-226861 None:intergenic 25.0%
! AGTTTCCATAATATCTAAAG+CGG + chr7_1:226941-226960 None:intergenic 25.0%
!!! TCAAGTAATGTGAGATTTTT+TGG - chr7_1:227044-227063 Msa1003170:intron 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG - chr7_1:227200-227219 Msa1003170:intron 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG + chr7_1:227281-227300 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG + chr7_1:227482-227501 None:intergenic 25.0%
! CACACAACAATATATACATA+TGG - chr7_1:226004-226023 Msa1003170:three_prime_UTR 25.0%
! CAAATTAAATATACCATCAG+TGG - chr7_1:226622-226641 Msa1003170:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG + chr7_1:226842-226861 None:intergenic 25.0%
! AGTTTCCATAATATCTAAAG+CGG + chr7_1:226941-226960 None:intergenic 25.0%
!!! TCAAGTAATGTGAGATTTTT+TGG - chr7_1:227044-227063 Msa1003170:intron 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG - chr7_1:227200-227219 Msa1003170:intron 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG + chr7_1:227281-227300 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG + chr7_1:227482-227501 None:intergenic 25.0%
ATATATTGTTGTGTGACAGT+TGG + chr7_1:225999-226018 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG + chr7_1:226249-226268 None:intergenic 30.0%
TGAGAAATTTATCTGTTCGT+TGG + chr7_1:226570-226589 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG - chr7_1:226626-226645 Msa1003170:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG + chr7_1:226839-226858 None:intergenic 30.0%
! ATTGTCCGCTTTAGATATTA+TGG - chr7_1:226933-226952 Msa1003170:CDS 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG - chr7_1:226983-227002 Msa1003170:intron 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG - chr7_1:226984-227003 Msa1003170:intron 30.0%
!! AGGTGCTATAGAATGAATTT+TGG + chr7_1:227089-227108 None:intergenic 30.0%
AATCCCAAACTTAATGAAAG+GGG + chr7_1:227129-227148 None:intergenic 30.0%
CAATCCCAAACTTAATGAAA+GGG + chr7_1:227130-227149 None:intergenic 30.0%
! CTTTCATTAAGTTTGGGATT+GGG - chr7_1:227129-227148 Msa1003170:intron 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG - chr7_1:227199-227218 Msa1003170:intron 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG - chr7_1:227201-227220 Msa1003170:intron 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG + chr7_1:227275-227294 None:intergenic 30.0%
ATATATTGTTGTGTGACAGT+TGG + chr7_1:225999-226018 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG + chr7_1:226249-226268 None:intergenic 30.0%
TGAGAAATTTATCTGTTCGT+TGG + chr7_1:226570-226589 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG - chr7_1:226626-226645 Msa1003170:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG + chr7_1:226839-226858 None:intergenic 30.0%
! ATTGTCCGCTTTAGATATTA+TGG - chr7_1:226933-226952 Msa1003170:CDS 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG - chr7_1:226983-227002 Msa1003170:intron 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG - chr7_1:226984-227003 Msa1003170:intron 30.0%
!! AGGTGCTATAGAATGAATTT+TGG + chr7_1:227089-227108 None:intergenic 30.0%
AATCCCAAACTTAATGAAAG+GGG + chr7_1:227129-227148 None:intergenic 30.0%
CAATCCCAAACTTAATGAAA+GGG + chr7_1:227130-227149 None:intergenic 30.0%
! CTTTCATTAAGTTTGGGATT+GGG - chr7_1:227129-227148 Msa1003170:intron 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG - chr7_1:227199-227218 Msa1003170:intron 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG - chr7_1:227201-227220 Msa1003170:intron 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG + chr7_1:227275-227294 None:intergenic 30.0%
CTTGATTCCAATATTCCACA+TGG - chr7_1:226050-226069 Msa1003170:three_prime_UTR 35.0%
ATTGGATCCATGTGGAATAT+TGG + chr7_1:226060-226079 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG - chr7_1:226060-226079 Msa1003170:three_prime_UTR 35.0%
AGAAGAAGGAAGAAACCAAT+TGG + chr7_1:226078-226097 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG + chr7_1:226123-226142 None:intergenic 35.0%
! ATGAGAAAGAACAAGGTACT+TGG + chr7_1:226242-226261 None:intergenic 35.0%
! GCTATTTAGCGCTGAATTAA+AGG - chr7_1:226295-226314 Msa1003170:three_prime_UTR 35.0%
ATGTATGGTGTATGAGATAC+TGG + chr7_1:226326-226345 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG - chr7_1:226328-226347 Msa1003170:three_prime_UTR 35.0%
AAACACTGACACATAAACAC+TGG + chr7_1:226416-226435 None:intergenic 35.0%
AGTGTTTATGTGTCAGTGTT+TGG - chr7_1:226415-226434 Msa1003170:three_prime_UTR 35.0%
GCGTCTATGTCTTAACTTTA+TGG - chr7_1:226437-226456 Msa1003170:three_prime_UTR 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG + chr7_1:226563-226582 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG - chr7_1:226593-226612 Msa1003170:exon 35.0%
AACTAACTGTGAAATTGCCA+TGG - chr7_1:226649-226668 Msa1003170:CDS 35.0%
AATCTGTGAATTACCATCCA+TGG + chr7_1:226669-226688 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG + chr7_1:226723-226742 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG - chr7_1:226796-226815 Msa1003170:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG - chr7_1:226797-226816 Msa1003170:CDS 35.0%
CTCACATTACTTGAAAATCG+AGG + chr7_1:227038-227057 None:intergenic 35.0%
CCAATCCCAAACTTAATGAA+AGG + chr7_1:227131-227150 None:intergenic 35.0%
! CCTTTCATTAAGTTTGGGAT+TGG - chr7_1:227128-227147 Msa1003170:intron 35.0%
ATTGAGTAAGAGGAAGAGAA+GGG + chr7_1:227158-227177 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG + chr7_1:227159-227178 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG + chr7_1:227168-227187 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG + chr7_1:227272-227291 None:intergenic 35.0%
CTTGATTCCAATATTCCACA+TGG - chr7_1:226050-226069 Msa1003170:three_prime_UTR 35.0%
ATTGGATCCATGTGGAATAT+TGG + chr7_1:226060-226079 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG - chr7_1:226060-226079 Msa1003170:three_prime_UTR 35.0%
AGAAGAAGGAAGAAACCAAT+TGG + chr7_1:226078-226097 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG + chr7_1:226123-226142 None:intergenic 35.0%
! ATGAGAAAGAACAAGGTACT+TGG + chr7_1:226242-226261 None:intergenic 35.0%
! GCTATTTAGCGCTGAATTAA+AGG - chr7_1:226295-226314 Msa1003170:three_prime_UTR 35.0%
ATGTATGGTGTATGAGATAC+TGG + chr7_1:226326-226345 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG - chr7_1:226328-226347 Msa1003170:three_prime_UTR 35.0%
AAACACTGACACATAAACAC+TGG + chr7_1:226416-226435 None:intergenic 35.0%
AGTGTTTATGTGTCAGTGTT+TGG - chr7_1:226415-226434 Msa1003170:three_prime_UTR 35.0%
GCGTCTATGTCTTAACTTTA+TGG - chr7_1:226437-226456 Msa1003170:three_prime_UTR 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG + chr7_1:226563-226582 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG - chr7_1:226593-226612 Msa1003170:exon 35.0%
AACTAACTGTGAAATTGCCA+TGG - chr7_1:226649-226668 Msa1003170:CDS 35.0%
AATCTGTGAATTACCATCCA+TGG + chr7_1:226669-226688 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG + chr7_1:226723-226742 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG - chr7_1:226796-226815 Msa1003170:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG - chr7_1:226797-226816 Msa1003170:CDS 35.0%
CTCACATTACTTGAAAATCG+AGG + chr7_1:227038-227057 None:intergenic 35.0%
CCAATCCCAAACTTAATGAA+AGG + chr7_1:227131-227150 None:intergenic 35.0%
! CCTTTCATTAAGTTTGGGAT+TGG - chr7_1:227128-227147 Msa1003170:intron 35.0%
ATTGAGTAAGAGGAAGAGAA+GGG + chr7_1:227158-227177 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG + chr7_1:227159-227178 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG + chr7_1:227168-227187 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG + chr7_1:227272-227291 None:intergenic 35.0%
AGAAACCAATTGGATCCATG+TGG + chr7_1:226068-226087 None:intergenic 40.0%
!! TCAACACTGATAGTGGTTGT+GGG + chr7_1:226147-226166 None:intergenic 40.0%
! ACAACCACTATCAGTGTTGA+GGG - chr7_1:226147-226166 Msa1003170:three_prime_UTR 40.0%
AATTCCCTCAACACTGATAG+TGG + chr7_1:226154-226173 None:intergenic 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG + chr7_1:226199-226218 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG - chr7_1:226210-226229 Msa1003170:three_prime_UTR 40.0%
GAATAGAACAACAGACAGAG+AGG - chr7_1:226592-226611 Msa1003170:exon 40.0%
AACTGTGAAATTGCCATGGA+TGG - chr7_1:226653-226672 Msa1003170:CDS 40.0%
CGGACAATACCTTAGAAAAG+TGG + chr7_1:226921-226940 None:intergenic 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG - chr7_1:227102-227121 Msa1003170:intron 40.0%
AACTTAATGAAAGGGGTCCA+AGG + chr7_1:227122-227141 None:intergenic 40.0%
TGGACCCCTTTCATTAAGTT+TGG - chr7_1:227122-227141 Msa1003170:intron 40.0%
GGACCCCTTTCATTAAGTTT+GGG - chr7_1:227123-227142 Msa1003170:intron 40.0%
ATTGAGAGTGGTGTAGGATA+AGG + chr7_1:227183-227202 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG + chr7_1:227195-227214 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG + chr7_1:227229-227248 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG + chr7_1:227266-227285 None:intergenic 40.0%
AGAAACCAATTGGATCCATG+TGG + chr7_1:226068-226087 None:intergenic 40.0%
!! TCAACACTGATAGTGGTTGT+GGG + chr7_1:226147-226166 None:intergenic 40.0%
! ACAACCACTATCAGTGTTGA+GGG - chr7_1:226147-226166 Msa1003170:three_prime_UTR 40.0%
AATTCCCTCAACACTGATAG+TGG + chr7_1:226154-226173 None:intergenic 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG + chr7_1:226199-226218 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG - chr7_1:226210-226229 Msa1003170:three_prime_UTR 40.0%
GAATAGAACAACAGACAGAG+AGG - chr7_1:226592-226611 Msa1003170:exon 40.0%
AACTGTGAAATTGCCATGGA+TGG - chr7_1:226653-226672 Msa1003170:CDS 40.0%
CGGACAATACCTTAGAAAAG+TGG + chr7_1:226921-226940 None:intergenic 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG - chr7_1:227102-227121 Msa1003170:intron 40.0%
AACTTAATGAAAGGGGTCCA+AGG + chr7_1:227122-227141 None:intergenic 40.0%
TGGACCCCTTTCATTAAGTT+TGG - chr7_1:227122-227141 Msa1003170:intron 40.0%
GGACCCCTTTCATTAAGTTT+GGG - chr7_1:227123-227142 Msa1003170:intron 40.0%
ATTGAGAGTGGTGTAGGATA+AGG + chr7_1:227183-227202 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG + chr7_1:227195-227214 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG + chr7_1:227229-227248 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG + chr7_1:227266-227285 None:intergenic 40.0%
ACAGCAGAGATGTCAGAAGA+AGG + chr7_1:226092-226111 None:intergenic 45.0%
!! CTCAACACTGATAGTGGTTG+TGG + chr7_1:226148-226167 None:intergenic 45.0%
CACAACCACTATCAGTGTTG+AGG - chr7_1:226146-226165 Msa1003170:three_prime_UTR 45.0%
TATTGTGATGCCTGCTCAAC+CGG - chr7_1:226177-226196 Msa1003170:three_prime_UTR 45.0%
! CAAGGTACTTGGTACATACC+TGG + chr7_1:226231-226250 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG + chr7_1:226341-226360 None:intergenic 45.0%
TGACACGATAACGAGACACA+TGG - chr7_1:226355-226374 Msa1003170:three_prime_UTR 45.0%
CAGTTAGTTCCATCCACTGA+TGG + chr7_1:226638-226657 None:intergenic 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG - chr7_1:226746-226765 Msa1003170:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG + chr7_1:226752-226771 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG + chr7_1:226802-226821 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG + chr7_1:226911-226930 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG + chr7_1:227109-227128 None:intergenic 45.0%
GGGTCCCCTAAAATAAAAGG+AGG + chr7_1:227208-227227 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG + chr7_1:227211-227230 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG + chr7_1:227228-227247 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG + chr7_1:227230-227249 None:intergenic 45.0%
ACAGCAGAGATGTCAGAAGA+AGG + chr7_1:226092-226111 None:intergenic 45.0%
!! CTCAACACTGATAGTGGTTG+TGG + chr7_1:226148-226167 None:intergenic 45.0%
CACAACCACTATCAGTGTTG+AGG - chr7_1:226146-226165 Msa1003170:three_prime_UTR 45.0%
TATTGTGATGCCTGCTCAAC+CGG - chr7_1:226177-226196 Msa1003170:three_prime_UTR 45.0%
! CAAGGTACTTGGTACATACC+TGG + chr7_1:226231-226250 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG + chr7_1:226341-226360 None:intergenic 45.0%
TGACACGATAACGAGACACA+TGG - chr7_1:226355-226374 Msa1003170:three_prime_UTR 45.0%
CAGTTAGTTCCATCCACTGA+TGG + chr7_1:226638-226657 None:intergenic 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG - chr7_1:226746-226765 Msa1003170:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG + chr7_1:226752-226771 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG + chr7_1:226802-226821 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG + chr7_1:226911-226930 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG + chr7_1:227109-227128 None:intergenic 45.0%
GGGTCCCCTAAAATAAAAGG+AGG + chr7_1:227208-227227 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG + chr7_1:227211-227230 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG + chr7_1:227228-227247 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG + chr7_1:227230-227249 None:intergenic 45.0%
TGTGACAGTTGGAGTGTGTG+CGG + chr7_1:225988-226007 None:intergenic 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG - chr7_1:226695-226714 Msa1003170:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG - chr7_1:226909-226928 Msa1003170:CDS 50.0%
GAGGTGATTGAGAGTGGTGT+AGG + chr7_1:227189-227208 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG - chr7_1:227215-227234 Msa1003170:intron 50.0%
GTCGAGAGAGAACACACAAG+AGG + chr7_1:227244-227263 None:intergenic 50.0%
GCTGCTAGCTAGCTAGCTAT+AGG + chr7_1:227350-227369 None:intergenic 50.0%
TGTGACAGTTGGAGTGTGTG+CGG + chr7_1:225988-226007 None:intergenic 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG - chr7_1:226695-226714 Msa1003170:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG - chr7_1:226909-226928 Msa1003170:CDS 50.0%
GAGGTGATTGAGAGTGGTGT+AGG + chr7_1:227189-227208 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG - chr7_1:227215-227234 Msa1003170:intron 50.0%
GTCGAGAGAGAACACACAAG+AGG + chr7_1:227244-227263 None:intergenic 50.0%
GCTGCTAGCTAGCTAGCTAT+AGG + chr7_1:227350-227369 None:intergenic 50.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG + chr7_1:226190-226209 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG + chr7_1:226903-226922 None:intergenic 55.0%
AGTTGGAGTGTGTGCGGTTC+AGG + chr7_1:225982-226001 None:intergenic 55.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG + chr7_1:226190-226209 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG + chr7_1:226903-226922 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 225981 227501 225981 ID=Msa1003170;Name=Msa1003170
chr7_1 mRNA 225981 227501 225981 ID=Msa1003170-mRNA-1;Parent=Msa1003170;Name=Msa1003170-mRNA-1;_AED=0.01;_eAED=0.01;_QI=88|1|1|1|1|1|2|624|173
chr7_1 exon 225981 226963 225981 ID=Msa1003170-mRNA-1:exon:40;Parent=Msa1003170-mRNA-1
chr7_1 exon 227251 227501 227251 ID=Msa1003170-mRNA-1:exon:39;Parent=Msa1003170-mRNA-1
chr7_1 five_prime_UTR 227414 227501 227414 ID=Msa1003170-mRNA-1:five_prime_utr;Parent=Msa1003170-mRNA-1
chr7_1 CDS 227251 227413 227251 ID=Msa1003170-mRNA-1:cds;Parent=Msa1003170-mRNA-1
chr7_1 CDS 226605 226963 226605 ID=Msa1003170-mRNA-1:cds;Parent=Msa1003170-mRNA-1
chr7_1 three_prime_UTR 225981 226604 225981 ID=Msa1003170-mRNA-1:three_prime_utr;Parent=Msa1003170-mRNA-1
Gene Sequence

>Msa1003170

ATGGATCCAATTGGTTTCTTCCTTCTTCTGACATCTCTGCTGTTCAGTTATCCATTTGTTCTCATTGCTCAATCTCCCACAACCACTATCAGTGTTGAGGGAATTGTTTATTGTGATGCCTGCTCAACCGGCACTTTCTCTAAACACAGTTATCTCTTGCCAGGTGTTGACGTTCATATAGAGTGCAGATTTAGAGCAAGCTCACCAAGAACCAACGAACAGATAAATTTCTCAGTGAATAGAACAACAGACAGAGAGGGAGCATACAAATTAAATATACCATCAGTGGATGGAACTAACTGTGAAATTGCCATGGATGGTAATTCACAGATTGTGACTCTCTGCGAAGCAAGCTTGATAGGAACCTCATCATCTTCTTATTCTTGCAATGTTCCTTTCTTGAAGAGCACAGGAAGTAGTGAGATATCAAAACAAGAGAATAACCTATGTGTATACAGCTTGGGAGCTCTTAGTTACAAGCCACCTGAAAATATGATCAATACTACCTTATGCTCAAATTAA
Protein sequence

>Msa1003170

MDPIGFFLLLTSLLFSYPFVLIAQSPTTTISVEGIVYCDACSTGTFSKHSYLLPGVDVHIECRFRASSPRTNEQINFSVNRTTDREGAYKLNIPSVDGTNCEIAMDGNSQIVTLCEASLIGTSSSSYSCNVPFLKSTGSSEISKQENNLCVYSLGALSYKPPENMINTTLCSN*