AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1133800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1133800 MtrunA17_Chr7g0214211 95.954 173 7 0 1 173 1 173 3.96e-113 318
Msa1133800 MtrunA17_Chr7g0224361 46.309 149 71 5 26 173 33 173 1.51e-41 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1133800 AT5G13140.1 50.667 150 66 5 26 173 35 178 5.75e-44 146
Msa1133800 AT5G41050.1 46.939 147 72 4 26 171 29 170 1.18e-37 127
Msa1133800 AT3G26960.1 43.478 138 69 3 26 161 33 163 4.39e-32 113

Find 73 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAATGAAGAAAGGATTT+TGG 0.210001 7_4:-10805 None:intergenic
TGATAGATGTAATTCATAAA+AGG 0.239602 7_4:-10914 None:intergenic
TGGACCCCTTTCATTAAGTT+TGG 0.252347 7_4:+10649 Msa1133800:three_prime_UTR
AGTGGCGTGCATCGGACTTT+TGG 0.261878 7_4:-10427 None:intergenic
TATGCTCAAATTAATTCATT+TGG 0.298493 7_4:+10391 Msa1133800:exon
ATTGGATCCATGTGGAATAT+TGG 0.305436 7_4:-9584 None:intergenic
AGGTGCTATAGAATGAATTT+TGG 0.319346 7_4:-10613 None:intergenic
ATTGTCCGCTTTAGATATTA+TGG 0.324237 7_4:+10460 Msa1133800:three_prime_UTR
TTTATCTGTTCGTTGGTTCT+TGG 0.331613 7_4:-10087 None:intergenic
GGACCCCTTTCATTAAGTTT+GGG 0.332848 7_4:+10650 Msa1133800:three_prime_UTR
CCTTTCATTAAGTTTGGGAT+TGG 0.340938 7_4:+10655 Msa1133800:three_prime_UTR
GTGGGGTCCCCTAAAATAAA+AGG 0.343139 7_4:-10735 None:intergenic
GGACATATCTATAAACAAAA+TGG 0.345515 7_4:-11006 None:intergenic
AATGAATTAATTTGAGCATA+AGG 0.365031 7_4:-10388 None:intergenic
GGATAAGGATATTGAGTAAG+AGG 0.392047 7_4:-10692 None:intergenic
TATTGTGATGCCTGCTCAAC+CGG 0.409183 7_4:+9704 Msa1133800:CDS
TATTGAGTAAGAGGAAGAGA+AGG 0.415307 7_4:-10683 None:intergenic
CTTTCATTAAGTTTGGGATT+GGG 0.420070 7_4:+10656 Msa1133800:three_prime_UTR
CTCTGCGAAGCAAGCTTGAT+AGG 0.425118 7_4:+10222 Msa1133800:CDS
CTCAACACTGATAGTGGTTG+TGG 0.425792 7_4:-9672 None:intergenic
ATTGAGAGTGGTGTAGGATA+AGG 0.427502 7_4:-10707 None:intergenic
GATTGAGCAATGAGAACAAA+TGG 0.438625 7_4:-9647 None:intergenic
AGAGAAAGTGCCGGTTGAGC+AGG 0.454772 7_4:-9714 None:intergenic
AGAAGAAGGAAGAAACCAAT+TGG 0.454824 7_4:-9602 None:intergenic
GCTGCTAGCTAGCTAGCTAT+AGG 0.455157 7_4:-10874 None:intergenic
GAGGTGATTGAGAGTGGTGT+AGG 0.457528 7_4:-10713 None:intergenic
AATTCCCTCAACACTGATAG+TGG 0.459627 7_4:-9678 None:intergenic
GATAGATGTAATTCATAAAA+GGG 0.468871 7_4:-10913 None:intergenic
AACCTATGTGTATACAGCTT+GGG 0.470069 7_4:+10324 Msa1133800:CDS
ATATTCCACATGGATCCAAT+TGG 0.475963 7_4:+9587 Msa1133800:exon
CAATCCCAAACTTAATGAAA+GGG 0.477690 7_4:-10654 None:intergenic
TGAGAAATTTATCTGTTCGT+TGG 0.493906 7_4:-10094 None:intergenic
CTTCCTGTGCTCTTCAAGAA+AGG 0.504211 7_4:-10276 None:intergenic
CACACAACAATATATACATA+TGG 0.507745 7_4:+9531 Msa1133800:five_prime_UTR
GTTCCTTTCTTGAAGAGCAC+AGG 0.511194 7_4:+10273 Msa1133800:CDS
TAACCTATGTGTATACAGCT+TGG 0.520473 7_4:+10323 Msa1133800:CDS
ATTGAGTAAGAGGAAGAGAA+GGG 0.521172 7_4:-10682 None:intergenic
AACTTAATGAAAGGGGTCCA+AGG 0.523943 7_4:-10646 None:intergenic
ACAACCACTATCAGTGTTGA+GGG 0.524635 7_4:+9674 Msa1133800:CDS
CCAATCCCAAACTTAATGAA+AGG 0.528525 7_4:-10655 None:intergenic
AAACACAGTTATCTCTTGCC+AGG 0.531682 7_4:+9737 Msa1133800:CDS
AAAGTTGTTGTTGAATTGAG+TGG 0.538831 7_4:+10510 Msa1133800:three_prime_UTR
ACAAGAGGAATCCAAGTTGT+GGG 0.540830 7_4:-10753 None:intergenic
CTTAGAAAAGTGGCGTGCAT+CGG 0.557367 7_4:-10435 None:intergenic
CAAATTAAATATACCATCAG+TGG 0.559042 7_4:+10149 Msa1133800:CDS
CAGTTAGTTCCATCCACTGA+TGG 0.559252 7_4:-10162 None:intergenic
CGGACAATACCTTAGAAAAG+TGG 0.567729 7_4:-10445 None:intergenic
CACAAGAGGAATCCAAGTTG+TGG 0.568323 7_4:-10754 None:intergenic
CACAACCACTATCAGTGTTG+AGG 0.573227 7_4:+9673 Msa1133800:CDS
CTTGATTCCAATATTCCACA+TGG 0.573944 7_4:+9577 Msa1133800:exon
TCAACACTGATAGTGGTTGT+GGG 0.576625 7_4:-9671 None:intergenic
AATCTGTGAATTACCATCCA+TGG 0.577346 7_4:-10193 None:intergenic
GGGTCCAAGGAAAGAAAAGA+AGG 0.578723 7_4:-10633 None:intergenic
ACAGCAGAGATGTCAGAAGA+AGG 0.579115 7_4:-9616 None:intergenic
AACTGTGAAATTGCCATGGA+TGG 0.583388 7_4:+10180 Msa1133800:CDS
ATATATTGTTGTGTGACAGT+TGG 0.583761 7_4:-9523 None:intergenic
TAAAAGGAGGTGATTGAGAG+TGG 0.589362 7_4:-10719 None:intergenic
TTAAATATACCATCAGTGGA+TGG 0.597816 7_4:+10153 Msa1133800:CDS
AACTAACTGTGAAATTGCCA+TGG 0.599482 7_4:+10176 Msa1133800:CDS
AAGTTGTTGTTGAATTGAGT+GGG 0.604852 7_4:+10511 Msa1133800:three_prime_UTR
CTCACATTACTTGAAAATCG+AGG 0.632356 7_4:-10562 None:intergenic
AGTTTCCATAATATCTAAAG+CGG 0.633242 7_4:-10465 None:intergenic
GCAAGAATAAGAAGATGATG+AGG 0.636237 7_4:-10247 None:intergenic
GAATAGAACAACAGACAGAG+AGG 0.636780 7_4:+10119 Msa1133800:CDS
CTCCCAAGCTGTATACACAT+AGG 0.650090 7_4:-10326 None:intergenic
TGTGACAGTTGGAGTGTGTG+CGG 0.650489 7_4:-9512 None:intergenic
GTCGAGAGAGAACACACAAG+AGG 0.653695 7_4:-10768 None:intergenic
AATCCCAAACTTAATGAAAG+GGG 0.655786 7_4:-10653 None:intergenic
AATAGAACAACAGACAGAGA+GGG 0.660031 7_4:+10120 Msa1133800:CDS
ACTGTGTTTAGAGAAAGTGC+CGG 0.671971 7_4:-9723 None:intergenic
GGGTCCCCTAAAATAAAAGG+AGG 0.676821 7_4:-10732 None:intergenic
CAAGAGGAATCCAAGTTGTG+GGG 0.691066 7_4:-10752 None:intergenic
AGAAACCAATTGGATCCATG+TGG 0.695775 7_4:-9592 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCATTTAATTATACATATAA+TGG - chr7_4:9804-9823 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG - chr7_4:10815-10834 None:intergenic 15.0%
!! GCATTTAATTATACATATAA+TGG - chr7_4:9804-9823 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG - chr7_4:10815-10834 None:intergenic 15.0%
!! TATCTATGATGATATATATC+AGG + chr7_4:10024-10043 Msa1133800:intron 20.0%
!!! AATGAATTAATTTGAGCATA+AGG - chr7_4:10391-10410 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG + chr7_4:10391-10410 Msa1133800:exon 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG - chr7_4:10601-10620 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG - chr7_4:10916-10935 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG - chr7_4:10917-10936 None:intergenic 20.0%
!! TATCTATGATGATATATATC+AGG + chr7_4:10024-10043 Msa1133800:intron 20.0%
!!! AATGAATTAATTTGAGCATA+AGG - chr7_4:10391-10410 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG + chr7_4:10391-10410 Msa1133800:exon 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG - chr7_4:10601-10620 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG - chr7_4:10916-10935 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG - chr7_4:10917-10936 None:intergenic 20.0%
! CACACAACAATATATACATA+TGG + chr7_4:9531-9550 Msa1133800:five_prime_UTR 25.0%
! CAAATTAAATATACCATCAG+TGG + chr7_4:10149-10168 Msa1133800:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG - chr7_4:10369-10388 None:intergenic 25.0%
! AGTTTCCATAATATCTAAAG+CGG - chr7_4:10468-10487 None:intergenic 25.0%
!!! TCAAGTAATGTGAGATTTTT+TGG + chr7_4:10571-10590 Msa1133800:three_prime_UTR 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG + chr7_4:10727-10746 Msa1133800:three_prime_UTR 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG - chr7_4:10808-10827 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG - chr7_4:11009-11028 None:intergenic 25.0%
! CACACAACAATATATACATA+TGG + chr7_4:9531-9550 Msa1133800:five_prime_UTR 25.0%
! CAAATTAAATATACCATCAG+TGG + chr7_4:10149-10168 Msa1133800:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG - chr7_4:10369-10388 None:intergenic 25.0%
! AGTTTCCATAATATCTAAAG+CGG - chr7_4:10468-10487 None:intergenic 25.0%
!!! TCAAGTAATGTGAGATTTTT+TGG + chr7_4:10571-10590 Msa1133800:three_prime_UTR 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG + chr7_4:10727-10746 Msa1133800:three_prime_UTR 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG - chr7_4:10808-10827 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG - chr7_4:11009-11028 None:intergenic 25.0%
ATATATTGTTGTGTGACAGT+TGG - chr7_4:9526-9545 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG - chr7_4:9776-9795 None:intergenic 30.0%
TGAGAAATTTATCTGTTCGT+TGG - chr7_4:10097-10116 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG + chr7_4:10153-10172 Msa1133800:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG - chr7_4:10366-10385 None:intergenic 30.0%
! ATTGTCCGCTTTAGATATTA+TGG + chr7_4:10460-10479 Msa1133800:three_prime_UTR 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG + chr7_4:10510-10529 Msa1133800:three_prime_UTR 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG + chr7_4:10511-10530 Msa1133800:three_prime_UTR 30.0%
!! AGGTGCTATAGAATGAATTT+TGG - chr7_4:10616-10635 None:intergenic 30.0%
AATCCCAAACTTAATGAAAG+GGG - chr7_4:10656-10675 None:intergenic 30.0%
CAATCCCAAACTTAATGAAA+GGG - chr7_4:10657-10676 None:intergenic 30.0%
! CTTTCATTAAGTTTGGGATT+GGG + chr7_4:10656-10675 Msa1133800:three_prime_UTR 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG + chr7_4:10726-10745 Msa1133800:three_prime_UTR 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG + chr7_4:10728-10747 Msa1133800:three_prime_UTR 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG - chr7_4:10802-10821 None:intergenic 30.0%
ATATATTGTTGTGTGACAGT+TGG - chr7_4:9526-9545 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG - chr7_4:9776-9795 None:intergenic 30.0%
TGAGAAATTTATCTGTTCGT+TGG - chr7_4:10097-10116 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG + chr7_4:10153-10172 Msa1133800:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG - chr7_4:10366-10385 None:intergenic 30.0%
! ATTGTCCGCTTTAGATATTA+TGG + chr7_4:10460-10479 Msa1133800:three_prime_UTR 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG + chr7_4:10510-10529 Msa1133800:three_prime_UTR 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG + chr7_4:10511-10530 Msa1133800:three_prime_UTR 30.0%
!! AGGTGCTATAGAATGAATTT+TGG - chr7_4:10616-10635 None:intergenic 30.0%
AATCCCAAACTTAATGAAAG+GGG - chr7_4:10656-10675 None:intergenic 30.0%
CAATCCCAAACTTAATGAAA+GGG - chr7_4:10657-10676 None:intergenic 30.0%
! CTTTCATTAAGTTTGGGATT+GGG + chr7_4:10656-10675 Msa1133800:three_prime_UTR 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG + chr7_4:10726-10745 Msa1133800:three_prime_UTR 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG + chr7_4:10728-10747 Msa1133800:three_prime_UTR 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG - chr7_4:10802-10821 None:intergenic 30.0%
CTTGATTCCAATATTCCACA+TGG + chr7_4:9577-9596 Msa1133800:exon 35.0%
ATTGGATCCATGTGGAATAT+TGG - chr7_4:9587-9606 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG + chr7_4:9587-9606 Msa1133800:exon 35.0%
AGAAGAAGGAAGAAACCAAT+TGG - chr7_4:9605-9624 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG - chr7_4:9650-9669 None:intergenic 35.0%
! ATGAGAAAGAACAAGGTACT+TGG - chr7_4:9769-9788 None:intergenic 35.0%
! GCTATTTAGCGCTGAATTAA+AGG + chr7_4:9822-9841 Msa1133800:intron 35.0%
ATGTATGGTGTATGAGATAC+TGG - chr7_4:9853-9872 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG + chr7_4:9855-9874 Msa1133800:intron 35.0%
AAACACTGACACATAAACAC+TGG - chr7_4:9943-9962 None:intergenic 35.0%
AGTGTTTATGTGTCAGTGTT+TGG + chr7_4:9942-9961 Msa1133800:intron 35.0%
GCGTCTATGTCTTAACTTTA+TGG + chr7_4:9964-9983 Msa1133800:intron 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG - chr7_4:10090-10109 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG + chr7_4:10120-10139 Msa1133800:CDS 35.0%
AACTAACTGTGAAATTGCCA+TGG + chr7_4:10176-10195 Msa1133800:CDS 35.0%
AATCTGTGAATTACCATCCA+TGG - chr7_4:10196-10215 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG - chr7_4:10250-10269 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG + chr7_4:10323-10342 Msa1133800:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG + chr7_4:10324-10343 Msa1133800:CDS 35.0%
CTCACATTACTTGAAAATCG+AGG - chr7_4:10565-10584 None:intergenic 35.0%
CCAATCCCAAACTTAATGAA+AGG - chr7_4:10658-10677 None:intergenic 35.0%
! CCTTTCATTAAGTTTGGGAT+TGG + chr7_4:10655-10674 Msa1133800:three_prime_UTR 35.0%
ATTGAGTAAGAGGAAGAGAA+GGG - chr7_4:10685-10704 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG - chr7_4:10686-10705 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG - chr7_4:10695-10714 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG - chr7_4:10799-10818 None:intergenic 35.0%
CTTGATTCCAATATTCCACA+TGG + chr7_4:9577-9596 Msa1133800:exon 35.0%
ATTGGATCCATGTGGAATAT+TGG - chr7_4:9587-9606 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG + chr7_4:9587-9606 Msa1133800:exon 35.0%
AGAAGAAGGAAGAAACCAAT+TGG - chr7_4:9605-9624 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG - chr7_4:9650-9669 None:intergenic 35.0%
! ATGAGAAAGAACAAGGTACT+TGG - chr7_4:9769-9788 None:intergenic 35.0%
! GCTATTTAGCGCTGAATTAA+AGG + chr7_4:9822-9841 Msa1133800:intron 35.0%
ATGTATGGTGTATGAGATAC+TGG - chr7_4:9853-9872 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG + chr7_4:9855-9874 Msa1133800:intron 35.0%
AAACACTGACACATAAACAC+TGG - chr7_4:9943-9962 None:intergenic 35.0%
AGTGTTTATGTGTCAGTGTT+TGG + chr7_4:9942-9961 Msa1133800:intron 35.0%
GCGTCTATGTCTTAACTTTA+TGG + chr7_4:9964-9983 Msa1133800:intron 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG - chr7_4:10090-10109 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG + chr7_4:10120-10139 Msa1133800:CDS 35.0%
AACTAACTGTGAAATTGCCA+TGG + chr7_4:10176-10195 Msa1133800:CDS 35.0%
AATCTGTGAATTACCATCCA+TGG - chr7_4:10196-10215 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG - chr7_4:10250-10269 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG + chr7_4:10323-10342 Msa1133800:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG + chr7_4:10324-10343 Msa1133800:CDS 35.0%
CTCACATTACTTGAAAATCG+AGG - chr7_4:10565-10584 None:intergenic 35.0%
CCAATCCCAAACTTAATGAA+AGG - chr7_4:10658-10677 None:intergenic 35.0%
! CCTTTCATTAAGTTTGGGAT+TGG + chr7_4:10655-10674 Msa1133800:three_prime_UTR 35.0%
ATTGAGTAAGAGGAAGAGAA+GGG - chr7_4:10685-10704 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG - chr7_4:10686-10705 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG - chr7_4:10695-10714 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG - chr7_4:10799-10818 None:intergenic 35.0%
AGAAACCAATTGGATCCATG+TGG - chr7_4:9595-9614 None:intergenic 40.0%
!! TCAACACTGATAGTGGTTGT+GGG - chr7_4:9674-9693 None:intergenic 40.0%
! ACAACCACTATCAGTGTTGA+GGG + chr7_4:9674-9693 Msa1133800:CDS 40.0%
AATTCCCTCAACACTGATAG+TGG - chr7_4:9681-9700 None:intergenic 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG - chr7_4:9726-9745 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG + chr7_4:9737-9756 Msa1133800:CDS 40.0%
GAATAGAACAACAGACAGAG+AGG + chr7_4:10119-10138 Msa1133800:CDS 40.0%
AACTGTGAAATTGCCATGGA+TGG + chr7_4:10180-10199 Msa1133800:CDS 40.0%
CGGACAATACCTTAGAAAAG+TGG - chr7_4:10448-10467 None:intergenic 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG + chr7_4:10629-10648 Msa1133800:three_prime_UTR 40.0%
AACTTAATGAAAGGGGTCCA+AGG - chr7_4:10649-10668 None:intergenic 40.0%
TGGACCCCTTTCATTAAGTT+TGG + chr7_4:10649-10668 Msa1133800:three_prime_UTR 40.0%
GGACCCCTTTCATTAAGTTT+GGG + chr7_4:10650-10669 Msa1133800:three_prime_UTR 40.0%
ATTGAGAGTGGTGTAGGATA+AGG - chr7_4:10710-10729 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG - chr7_4:10722-10741 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG - chr7_4:10756-10775 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG - chr7_4:10793-10812 None:intergenic 40.0%
AGAAACCAATTGGATCCATG+TGG - chr7_4:9595-9614 None:intergenic 40.0%
!! TCAACACTGATAGTGGTTGT+GGG - chr7_4:9674-9693 None:intergenic 40.0%
! ACAACCACTATCAGTGTTGA+GGG + chr7_4:9674-9693 Msa1133800:CDS 40.0%
AATTCCCTCAACACTGATAG+TGG - chr7_4:9681-9700 None:intergenic 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG - chr7_4:9726-9745 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG + chr7_4:9737-9756 Msa1133800:CDS 40.0%
GAATAGAACAACAGACAGAG+AGG + chr7_4:10119-10138 Msa1133800:CDS 40.0%
AACTGTGAAATTGCCATGGA+TGG + chr7_4:10180-10199 Msa1133800:CDS 40.0%
CGGACAATACCTTAGAAAAG+TGG - chr7_4:10448-10467 None:intergenic 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG + chr7_4:10629-10648 Msa1133800:three_prime_UTR 40.0%
AACTTAATGAAAGGGGTCCA+AGG - chr7_4:10649-10668 None:intergenic 40.0%
TGGACCCCTTTCATTAAGTT+TGG + chr7_4:10649-10668 Msa1133800:three_prime_UTR 40.0%
GGACCCCTTTCATTAAGTTT+GGG + chr7_4:10650-10669 Msa1133800:three_prime_UTR 40.0%
ATTGAGAGTGGTGTAGGATA+AGG - chr7_4:10710-10729 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG - chr7_4:10722-10741 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG - chr7_4:10756-10775 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG - chr7_4:10793-10812 None:intergenic 40.0%
ACAGCAGAGATGTCAGAAGA+AGG - chr7_4:9619-9638 None:intergenic 45.0%
!! CTCAACACTGATAGTGGTTG+TGG - chr7_4:9675-9694 None:intergenic 45.0%
CACAACCACTATCAGTGTTG+AGG + chr7_4:9673-9692 Msa1133800:CDS 45.0%
TATTGTGATGCCTGCTCAAC+CGG + chr7_4:9704-9723 Msa1133800:CDS 45.0%
! CAAGGTACTTGGTACATACC+TGG - chr7_4:9758-9777 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG - chr7_4:9868-9887 None:intergenic 45.0%
TGACACGATAACGAGACACA+TGG + chr7_4:9882-9901 Msa1133800:intron 45.0%
CAGTTAGTTCCATCCACTGA+TGG - chr7_4:10165-10184 None:intergenic 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG + chr7_4:10273-10292 Msa1133800:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG - chr7_4:10279-10298 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG - chr7_4:10329-10348 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG - chr7_4:10438-10457 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG - chr7_4:10636-10655 None:intergenic 45.0%
GGGTCCCCTAAAATAAAAGG+AGG - chr7_4:10735-10754 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG - chr7_4:10738-10757 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG - chr7_4:10755-10774 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG - chr7_4:10757-10776 None:intergenic 45.0%
ACAGCAGAGATGTCAGAAGA+AGG - chr7_4:9619-9638 None:intergenic 45.0%
!! CTCAACACTGATAGTGGTTG+TGG - chr7_4:9675-9694 None:intergenic 45.0%
CACAACCACTATCAGTGTTG+AGG + chr7_4:9673-9692 Msa1133800:CDS 45.0%
TATTGTGATGCCTGCTCAAC+CGG + chr7_4:9704-9723 Msa1133800:CDS 45.0%
! CAAGGTACTTGGTACATACC+TGG - chr7_4:9758-9777 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG - chr7_4:9868-9887 None:intergenic 45.0%
TGACACGATAACGAGACACA+TGG + chr7_4:9882-9901 Msa1133800:intron 45.0%
CAGTTAGTTCCATCCACTGA+TGG - chr7_4:10165-10184 None:intergenic 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG + chr7_4:10273-10292 Msa1133800:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG - chr7_4:10279-10298 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG - chr7_4:10329-10348 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG - chr7_4:10438-10457 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG - chr7_4:10636-10655 None:intergenic 45.0%
GGGTCCCCTAAAATAAAAGG+AGG - chr7_4:10735-10754 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG - chr7_4:10738-10757 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG - chr7_4:10755-10774 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG - chr7_4:10757-10776 None:intergenic 45.0%
TGTGACAGTTGGAGTGTGTG+CGG - chr7_4:9515-9534 None:intergenic 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG + chr7_4:10222-10241 Msa1133800:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG + chr7_4:10436-10455 Msa1133800:three_prime_UTR 50.0%
GAGGTGATTGAGAGTGGTGT+AGG - chr7_4:10716-10735 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG + chr7_4:10742-10761 Msa1133800:three_prime_UTR 50.0%
GTCGAGAGAGAACACACAAG+AGG - chr7_4:10771-10790 None:intergenic 50.0%
GCTGCTAGCTAGCTAGCTAT+AGG - chr7_4:10877-10896 None:intergenic 50.0%
TGTGACAGTTGGAGTGTGTG+CGG - chr7_4:9515-9534 None:intergenic 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG + chr7_4:10222-10241 Msa1133800:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG + chr7_4:10436-10455 Msa1133800:three_prime_UTR 50.0%
GAGGTGATTGAGAGTGGTGT+AGG - chr7_4:10716-10735 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG + chr7_4:10742-10761 Msa1133800:three_prime_UTR 50.0%
GTCGAGAGAGAACACACAAG+AGG - chr7_4:10771-10790 None:intergenic 50.0%
GCTGCTAGCTAGCTAGCTAT+AGG - chr7_4:10877-10896 None:intergenic 50.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG - chr7_4:9717-9736 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG - chr7_4:10430-10449 None:intergenic 55.0%
AGTTGGAGTGTGTGCGGTTC+AGG - chr7_4:9509-9528 None:intergenic 55.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG - chr7_4:9717-9736 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG - chr7_4:10430-10449 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 9508 11028 9508 ID=Msa1133800;Name=Msa1133800
chr7_4 mRNA 9508 11028 9508 ID=Msa1133800-mRNA-1;Parent=Msa1133800;Name=Msa1133800-mRNA-1;_AED=0.01;_eAED=0.01;_QI=88|1|1|1|1|1|2|624|173
chr7_4 exon 9508 9758 9508 ID=Msa1133800-mRNA-1:exon:0;Parent=Msa1133800-mRNA-1
chr7_4 exon 10046 11028 10046 ID=Msa1133800-mRNA-1:exon:1;Parent=Msa1133800-mRNA-1
chr7_4 five_prime_UTR 9508 9595 9508 ID=Msa1133800-mRNA-1:five_prime_utr;Parent=Msa1133800-mRNA-1
chr7_4 CDS 9596 9758 9596 ID=Msa1133800-mRNA-1:cds;Parent=Msa1133800-mRNA-1
chr7_4 CDS 10046 10404 10046 ID=Msa1133800-mRNA-1:cds;Parent=Msa1133800-mRNA-1
chr7_4 three_prime_UTR 10405 11028 10405 ID=Msa1133800-mRNA-1:three_prime_utr;Parent=Msa1133800-mRNA-1
Gene Sequence

>Msa1133800

ATGGATCCAATTGGTTTCTTCCTTCTTCTGACATCTCTGCTGTTCAGTTATCCATTTGTTCTCATTGCTCAATCTCCCACAACCACTATCAGTGTTGAGGGAATTGTTTATTGTGATGCCTGCTCAACCGGCACTTTCTCTAAACACAGTTATCTCTTGCCAGGTGTTGACGTTCATATAGAGTGCAGATTTAGAGCAAGCTCACCAAGAACCAACGAACAGATAAATTTCTCAGTGAATAGAACAACAGACAGAGAGGGAGCATACAAATTAAATATACCATCAGTGGATGGAACTAACTGTGAAATTGCCATGGATGGTAATTCACAGATTGTGACTCTCTGCGAAGCAAGCTTGATAGGAACCTCATCATCTTCTTATTCTTGCAATGTTCCTTTCTTGAAGAGCACAGGAAGTAGTGAGATATCAAAACAAGAGAATAACCTATGTGTATACAGCTTGGGAGCTCTTAGTTACAAGCCACCTGAAAATATGATCAATACTACCTTATGCTCAAATTAA
Protein sequence

>Msa1133800

MDPIGFFLLLTSLLFSYPFVLIAQSPTTTISVEGIVYCDACSTGTFSKHSYLLPGVDVHIECRFRASSPRTNEQINFSVNRTTDREGAYKLNIPSVDGTNCEIAMDGNSQIVTLCEASLIGTSSSSYSCNVPFLKSTGSSEISKQENNLCVYSLGALSYKPPENMINTTLCSN*