AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1046240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1046240 MtrunA17_Chr7g0214211 95.954 173 7 0 1 173 1 173 3.96e-113 318
Msa1046240 MtrunA17_Chr7g0224361 46.309 149 71 5 26 173 33 173 1.51e-41 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1046240 AT5G13140.1 50.667 150 66 5 26 173 35 178 5.75e-44 146
Msa1046240 AT5G41050.1 46.939 147 72 4 26 171 29 170 1.18e-37 127
Msa1046240 AT3G26960.1 43.478 138 69 3 26 161 33 163 4.39e-32 113

Find 71 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACCCCTTTCATTAAATT+TGG 0.111985 7_2:+6741872 Msa1046240:three_prime_UTR
AGAAAATGAAGAAAGGATTT+TGG 0.210001 7_2:-6742028 None:intergenic
TGATAGATGTAATTCATAAA+AGG 0.239602 7_2:-6742135 None:intergenic
AGTGGCGTGCATCGGACTTT+TGG 0.261878 7_2:-6741650 None:intergenic
TATGCTCAAATTAATTCATT+TGG 0.298493 7_2:+6741614 Msa1046240:exon
ATTGGATCCATGTGGAATAT+TGG 0.305436 7_2:-6740807 None:intergenic
AGGTGCTATAGAATGAATTT+TGG 0.319346 7_2:-6741836 None:intergenic
ATTGTCCGCTTTAGATATTA+TGG 0.324237 7_2:+6741683 Msa1046240:three_prime_UTR
TTTATCTGTTCGTTGGTTCT+TGG 0.331613 7_2:-6741310 None:intergenic
GGACCCCTTTCATTAAATTT+GGG 0.340081 7_2:+6741873 Msa1046240:three_prime_UTR
GTGGGGTCCCCTAAAATAAA+AGG 0.343139 7_2:-6741958 None:intergenic
GGACATATCTATAAACAAAA+TGG 0.355970 7_2:-6742227 None:intergenic
AATGAATTAATTTGAGCATA+AGG 0.365031 7_2:-6741611 None:intergenic
CCTTTCATTAAATTTGGGAT+TGG 0.379687 7_2:+6741878 Msa1046240:three_prime_UTR
CTTTCATTAAATTTGGGATT+GGG 0.386274 7_2:+6741879 Msa1046240:three_prime_UTR
GGATAAGGATATTGAGTAAG+AGG 0.392047 7_2:-6741915 None:intergenic
TATTGTGATGCCTGCTCAAC+CGG 0.409183 7_2:+6740927 Msa1046240:CDS
TATTGAGTAAGAGGAAGAGA+AGG 0.415307 7_2:-6741906 None:intergenic
CTCTGCGAAGCAAGCTTGAT+AGG 0.425118 7_2:+6741445 Msa1046240:CDS
ATTGAGAGTGGTGTAGGATA+AGG 0.427502 7_2:-6741930 None:intergenic
CAATCCCAAATTTAATGAAA+GGG 0.432334 7_2:-6741877 None:intergenic
GATTGAGCAATGAGAACAAA+TGG 0.438625 7_2:-6740870 None:intergenic
AGAGAAAGTGCCGGTTGAGC+AGG 0.454772 7_2:-6740937 None:intergenic
AGAAGAAGGAAGAAACCAAT+TGG 0.454824 7_2:-6740825 None:intergenic
GAGGTGATTGAGAGTGGTGT+AGG 0.457528 7_2:-6741936 None:intergenic
GATAGATGTAATTCATAAAA+GGG 0.468871 7_2:-6742134 None:intergenic
AACCTATGTGTATACAGCTT+GGG 0.470069 7_2:+6741547 Msa1046240:CDS
ATATTCCACATGGATCCAAT+TGG 0.475963 7_2:+6740810 Msa1046240:exon
CAGCTGCTAGCTAGCTAGCT+AGG 0.480010 7_2:-6742097 None:intergenic
TGAGAAATTTATCTGTTCGT+TGG 0.493906 7_2:-6741317 None:intergenic
CTTCCTGTGCTCTTCAAGAA+AGG 0.504211 7_2:-6741499 None:intergenic
GTTCCTTTCTTGAAGAGCAC+AGG 0.511194 7_2:+6741496 Msa1046240:CDS
ACAACTACTATCAGTGTTGA+GGG 0.511809 7_2:+6740897 Msa1046240:CDS
CTCAACACTGATAGTAGTTG+TGG 0.512743 7_2:-6740895 None:intergenic
CCAATCCCAAATTTAATGAA+AGG 0.513892 7_2:-6741878 None:intergenic
TAACCTATGTGTATACAGCT+TGG 0.520473 7_2:+6741546 Msa1046240:CDS
ATTGAGTAAGAGGAAGAGAA+GGG 0.521172 7_2:-6741905 None:intergenic
AAACACAGTTATCTCTTGCC+AGG 0.531682 7_2:+6740960 Msa1046240:CDS
AAAGTTGTTGTTGAATTGAG+TGG 0.538831 7_2:+6741733 Msa1046240:three_prime_UTR
ACAAGAGGAATCCAAGTTGT+GGG 0.540830 7_2:-6741976 None:intergenic
CAGTTAGTTCCATCCACTGA+TGG 0.541304 7_2:-6741385 None:intergenic
CTTAGAAAAGTGGCGTGCAT+CGG 0.557367 7_2:-6741658 None:intergenic
CAAATTAAATATACCATCAG+TGG 0.559042 7_2:+6741372 Msa1046240:CDS
CGGACAATACCTTAGAAAAG+TGG 0.567729 7_2:-6741668 None:intergenic
CACAAGAGGAATCCAAGTTG+TGG 0.568323 7_2:-6741977 None:intergenic
CACAACTACTATCAGTGTTG+AGG 0.571055 7_2:+6740896 Msa1046240:CDS
CTTGATTCCAATATTCCACA+TGG 0.573944 7_2:+6740800 Msa1046240:exon
AACTGCGAAATTGCCATGGA+TGG 0.574094 7_2:+6741403 Msa1046240:CDS
AATCTGTGAATTACCATCCA+TGG 0.577346 7_2:-6741416 None:intergenic
GGGTCCAAGGAAAGAAAAGA+AGG 0.578723 7_2:-6741856 None:intergenic
ACAGCAGAGATGTCAGAAGA+AGG 0.579115 7_2:-6740839 None:intergenic
TCAACACTGATAGTAGTTGT+GGG 0.582910 7_2:-6740894 None:intergenic
AATTTAATGAAAGGGGTCCA+AGG 0.582976 7_2:-6741869 None:intergenic
ATATATTGTTGTGTGACAGT+TGG 0.583761 7_2:-6740746 None:intergenic
TAAAAGGAGGTGATTGAGAG+TGG 0.589362 7_2:-6741942 None:intergenic
AACTAACTGCGAAATTGCCA+TGG 0.592115 7_2:+6741399 Msa1046240:CDS
TTAAATATACCATCAGTGGA+TGG 0.597816 7_2:+6741376 Msa1046240:CDS
AATCCCAAATTTAATGAAAG+GGG 0.600236 7_2:-6741876 None:intergenic
AAGTTGTTGTTGAATTGAGT+GGG 0.604852 7_2:+6741734 Msa1046240:three_prime_UTR
CTCACATTACTTGAAAATCG+AGG 0.632356 7_2:-6741785 None:intergenic
AGTTTCCATAATATCTAAAG+CGG 0.633242 7_2:-6741688 None:intergenic
GCAAGAATAAGAAGATGATG+AGG 0.636237 7_2:-6741470 None:intergenic
GAATAGAACAACAGACAGAG+AGG 0.636780 7_2:+6741342 Msa1046240:CDS
CTCCCAAGCTGTATACACAT+AGG 0.650090 7_2:-6741549 None:intergenic
TGTGACAGTTGGAGTGTGTG+CGG 0.650489 7_2:-6740735 None:intergenic
GTCGAGAGAGAACACACAAG+AGG 0.653695 7_2:-6741991 None:intergenic
AATAGAACAACAGACAGAGA+GGG 0.660031 7_2:+6741343 Msa1046240:CDS
ACTGTGTTTAGAGAAAGTGC+CGG 0.671971 7_2:-6740946 None:intergenic
GGGTCCCCTAAAATAAAAGG+AGG 0.676821 7_2:-6741955 None:intergenic
CAAGAGGAATCCAAGTTGTG+GGG 0.691066 7_2:-6741975 None:intergenic
AGAAACCAATTGGATCCATG+TGG 0.695775 7_2:-6740815 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCATTTAATTATACATATAA+TGG - chr7_2:6741027-6741046 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG - chr7_2:6742038-6742057 None:intergenic 15.0%
!! GCATTTAATTATACATATAA+TGG - chr7_2:6741027-6741046 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG - chr7_2:6742038-6742057 None:intergenic 15.0%
!! TATCTATGATGATATATATC+AGG + chr7_2:6741247-6741266 Msa1046240:intron 20.0%
!!! AATGAATTAATTTGAGCATA+AGG - chr7_2:6741614-6741633 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG + chr7_2:6741614-6741633 Msa1046240:exon 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG - chr7_2:6741824-6741843 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG - chr7_2:6742137-6742156 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG - chr7_2:6742138-6742157 None:intergenic 20.0%
!! TATCTATGATGATATATATC+AGG + chr7_2:6741247-6741266 Msa1046240:intron 20.0%
!!! AATGAATTAATTTGAGCATA+AGG - chr7_2:6741614-6741633 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG + chr7_2:6741614-6741633 Msa1046240:exon 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG - chr7_2:6741824-6741843 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG - chr7_2:6742137-6742156 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG - chr7_2:6742138-6742157 None:intergenic 20.0%
! CAAATTAAATATACCATCAG+TGG + chr7_2:6741372-6741391 Msa1046240:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG - chr7_2:6741592-6741611 None:intergenic 25.0%
! AGTTTCCATAATATCTAAAG+CGG - chr7_2:6741691-6741710 None:intergenic 25.0%
!!! TCAAGTAATGTGAGATTTTT+TGG + chr7_2:6741794-6741813 Msa1046240:three_prime_UTR 25.0%
! AATCCCAAATTTAATGAAAG+GGG - chr7_2:6741879-6741898 None:intergenic 25.0%
! CAATCCCAAATTTAATGAAA+GGG - chr7_2:6741880-6741899 None:intergenic 25.0%
!! CTTTCATTAAATTTGGGATT+GGG + chr7_2:6741879-6741898 Msa1046240:three_prime_UTR 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG + chr7_2:6741950-6741969 Msa1046240:three_prime_UTR 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG - chr7_2:6742031-6742050 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG - chr7_2:6742230-6742249 None:intergenic 25.0%
! CAAATTAAATATACCATCAG+TGG + chr7_2:6741372-6741391 Msa1046240:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG - chr7_2:6741592-6741611 None:intergenic 25.0%
! AGTTTCCATAATATCTAAAG+CGG - chr7_2:6741691-6741710 None:intergenic 25.0%
!!! TCAAGTAATGTGAGATTTTT+TGG + chr7_2:6741794-6741813 Msa1046240:three_prime_UTR 25.0%
! AATCCCAAATTTAATGAAAG+GGG - chr7_2:6741879-6741898 None:intergenic 25.0%
! CAATCCCAAATTTAATGAAA+GGG - chr7_2:6741880-6741899 None:intergenic 25.0%
!! CTTTCATTAAATTTGGGATT+GGG + chr7_2:6741879-6741898 Msa1046240:three_prime_UTR 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG + chr7_2:6741950-6741969 Msa1046240:three_prime_UTR 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG - chr7_2:6742031-6742050 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG - chr7_2:6742230-6742249 None:intergenic 25.0%
ATATATTGTTGTGTGACAGT+TGG - chr7_2:6740749-6740768 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG - chr7_2:6740999-6741018 None:intergenic 30.0%
! GCTATTTAGCGTTGAATTAA+AGG + chr7_2:6741045-6741064 Msa1046240:intron 30.0%
GTGTCTATGTCTTAACTTTA+TGG + chr7_2:6741187-6741206 Msa1046240:intron 30.0%
TGAGAAATTTATCTGTTCGT+TGG - chr7_2:6741320-6741339 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG + chr7_2:6741376-6741395 Msa1046240:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG - chr7_2:6741589-6741608 None:intergenic 30.0%
! ATTGTCCGCTTTAGATATTA+TGG + chr7_2:6741683-6741702 Msa1046240:three_prime_UTR 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG + chr7_2:6741733-6741752 Msa1046240:three_prime_UTR 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG + chr7_2:6741734-6741753 Msa1046240:three_prime_UTR 30.0%
!! AGGTGCTATAGAATGAATTT+TGG - chr7_2:6741839-6741858 None:intergenic 30.0%
CCAATCCCAAATTTAATGAA+AGG - chr7_2:6741881-6741900 None:intergenic 30.0%
! CCTTTCATTAAATTTGGGAT+TGG + chr7_2:6741878-6741897 Msa1046240:three_prime_UTR 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG + chr7_2:6741949-6741968 Msa1046240:three_prime_UTR 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG + chr7_2:6741951-6741970 Msa1046240:three_prime_UTR 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG - chr7_2:6742025-6742044 None:intergenic 30.0%
ATATATTGTTGTGTGACAGT+TGG - chr7_2:6740749-6740768 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG - chr7_2:6740999-6741018 None:intergenic 30.0%
! GCTATTTAGCGTTGAATTAA+AGG + chr7_2:6741045-6741064 Msa1046240:intron 30.0%
GTGTCTATGTCTTAACTTTA+TGG + chr7_2:6741187-6741206 Msa1046240:intron 30.0%
TGAGAAATTTATCTGTTCGT+TGG - chr7_2:6741320-6741339 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG + chr7_2:6741376-6741395 Msa1046240:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG - chr7_2:6741589-6741608 None:intergenic 30.0%
! ATTGTCCGCTTTAGATATTA+TGG + chr7_2:6741683-6741702 Msa1046240:three_prime_UTR 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG + chr7_2:6741733-6741752 Msa1046240:three_prime_UTR 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG + chr7_2:6741734-6741753 Msa1046240:three_prime_UTR 30.0%
!! AGGTGCTATAGAATGAATTT+TGG - chr7_2:6741839-6741858 None:intergenic 30.0%
CCAATCCCAAATTTAATGAA+AGG - chr7_2:6741881-6741900 None:intergenic 30.0%
! CCTTTCATTAAATTTGGGAT+TGG + chr7_2:6741878-6741897 Msa1046240:three_prime_UTR 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG + chr7_2:6741949-6741968 Msa1046240:three_prime_UTR 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG + chr7_2:6741951-6741970 Msa1046240:three_prime_UTR 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG - chr7_2:6742025-6742044 None:intergenic 30.0%
CTTGATTCCAATATTCCACA+TGG + chr7_2:6740800-6740819 Msa1046240:exon 35.0%
ATTGGATCCATGTGGAATAT+TGG - chr7_2:6740810-6740829 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG + chr7_2:6740810-6740829 Msa1046240:exon 35.0%
AGAAGAAGGAAGAAACCAAT+TGG - chr7_2:6740828-6740847 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG - chr7_2:6740873-6740892 None:intergenic 35.0%
TCAACACTGATAGTAGTTGT+GGG - chr7_2:6740897-6740916 None:intergenic 35.0%
! ACAACTACTATCAGTGTTGA+GGG + chr7_2:6740897-6740916 Msa1046240:CDS 35.0%
! ATGAGAAAGAACAAGGTACT+TGG - chr7_2:6740992-6741011 None:intergenic 35.0%
ATGTATGGTGTATGAGATAC+TGG - chr7_2:6741076-6741095 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG + chr7_2:6741078-6741097 Msa1046240:intron 35.0%
AGTGTTTATGTGTCAGTGTT+TGG + chr7_2:6741165-6741184 Msa1046240:intron 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG - chr7_2:6741313-6741332 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG + chr7_2:6741343-6741362 Msa1046240:CDS 35.0%
AATCTGTGAATTACCATCCA+TGG - chr7_2:6741419-6741438 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG - chr7_2:6741473-6741492 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG + chr7_2:6741546-6741565 Msa1046240:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG + chr7_2:6741547-6741566 Msa1046240:CDS 35.0%
CTCACATTACTTGAAAATCG+AGG - chr7_2:6741788-6741807 None:intergenic 35.0%
AATTTAATGAAAGGGGTCCA+AGG - chr7_2:6741872-6741891 None:intergenic 35.0%
TGGACCCCTTTCATTAAATT+TGG + chr7_2:6741872-6741891 Msa1046240:three_prime_UTR 35.0%
GGACCCCTTTCATTAAATTT+GGG + chr7_2:6741873-6741892 Msa1046240:three_prime_UTR 35.0%
ATTGAGTAAGAGGAAGAGAA+GGG - chr7_2:6741908-6741927 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG - chr7_2:6741909-6741928 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG - chr7_2:6741918-6741937 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG - chr7_2:6742022-6742041 None:intergenic 35.0%
CTTGATTCCAATATTCCACA+TGG + chr7_2:6740800-6740819 Msa1046240:exon 35.0%
ATTGGATCCATGTGGAATAT+TGG - chr7_2:6740810-6740829 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG + chr7_2:6740810-6740829 Msa1046240:exon 35.0%
AGAAGAAGGAAGAAACCAAT+TGG - chr7_2:6740828-6740847 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG - chr7_2:6740873-6740892 None:intergenic 35.0%
TCAACACTGATAGTAGTTGT+GGG - chr7_2:6740897-6740916 None:intergenic 35.0%
! ACAACTACTATCAGTGTTGA+GGG + chr7_2:6740897-6740916 Msa1046240:CDS 35.0%
! ATGAGAAAGAACAAGGTACT+TGG - chr7_2:6740992-6741011 None:intergenic 35.0%
ATGTATGGTGTATGAGATAC+TGG - chr7_2:6741076-6741095 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG + chr7_2:6741078-6741097 Msa1046240:intron 35.0%
AGTGTTTATGTGTCAGTGTT+TGG + chr7_2:6741165-6741184 Msa1046240:intron 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG - chr7_2:6741313-6741332 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG + chr7_2:6741343-6741362 Msa1046240:CDS 35.0%
AATCTGTGAATTACCATCCA+TGG - chr7_2:6741419-6741438 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG - chr7_2:6741473-6741492 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG + chr7_2:6741546-6741565 Msa1046240:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG + chr7_2:6741547-6741566 Msa1046240:CDS 35.0%
CTCACATTACTTGAAAATCG+AGG - chr7_2:6741788-6741807 None:intergenic 35.0%
AATTTAATGAAAGGGGTCCA+AGG - chr7_2:6741872-6741891 None:intergenic 35.0%
TGGACCCCTTTCATTAAATT+TGG + chr7_2:6741872-6741891 Msa1046240:three_prime_UTR 35.0%
GGACCCCTTTCATTAAATTT+GGG + chr7_2:6741873-6741892 Msa1046240:three_prime_UTR 35.0%
ATTGAGTAAGAGGAAGAGAA+GGG - chr7_2:6741908-6741927 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG - chr7_2:6741909-6741928 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG - chr7_2:6741918-6741937 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG - chr7_2:6742022-6742041 None:intergenic 35.0%
AGAAACCAATTGGATCCATG+TGG - chr7_2:6740818-6740837 None:intergenic 40.0%
CTCAACACTGATAGTAGTTG+TGG - chr7_2:6740898-6740917 None:intergenic 40.0%
CACAACTACTATCAGTGTTG+AGG + chr7_2:6740896-6740915 Msa1046240:CDS 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG - chr7_2:6740949-6740968 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG + chr7_2:6740960-6740979 Msa1046240:CDS 40.0%
TGACACGATAATGAGACACA+TGG + chr7_2:6741105-6741124 Msa1046240:intron 40.0%
GAATAGAACAACAGACAGAG+AGG + chr7_2:6741342-6741361 Msa1046240:CDS 40.0%
AACTAACTGCGAAATTGCCA+TGG + chr7_2:6741399-6741418 Msa1046240:CDS 40.0%
CGGACAATACCTTAGAAAAG+TGG - chr7_2:6741671-6741690 None:intergenic 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG + chr7_2:6741852-6741871 Msa1046240:three_prime_UTR 40.0%
ATTGAGAGTGGTGTAGGATA+AGG - chr7_2:6741933-6741952 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG - chr7_2:6741945-6741964 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG - chr7_2:6741979-6741998 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG - chr7_2:6742016-6742035 None:intergenic 40.0%
AGAAACCAATTGGATCCATG+TGG - chr7_2:6740818-6740837 None:intergenic 40.0%
CTCAACACTGATAGTAGTTG+TGG - chr7_2:6740898-6740917 None:intergenic 40.0%
CACAACTACTATCAGTGTTG+AGG + chr7_2:6740896-6740915 Msa1046240:CDS 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG - chr7_2:6740949-6740968 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG + chr7_2:6740960-6740979 Msa1046240:CDS 40.0%
TGACACGATAATGAGACACA+TGG + chr7_2:6741105-6741124 Msa1046240:intron 40.0%
GAATAGAACAACAGACAGAG+AGG + chr7_2:6741342-6741361 Msa1046240:CDS 40.0%
AACTAACTGCGAAATTGCCA+TGG + chr7_2:6741399-6741418 Msa1046240:CDS 40.0%
CGGACAATACCTTAGAAAAG+TGG - chr7_2:6741671-6741690 None:intergenic 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG + chr7_2:6741852-6741871 Msa1046240:three_prime_UTR 40.0%
ATTGAGAGTGGTGTAGGATA+AGG - chr7_2:6741933-6741952 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG - chr7_2:6741945-6741964 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG - chr7_2:6741979-6741998 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG - chr7_2:6742016-6742035 None:intergenic 40.0%
ACAGCAGAGATGTCAGAAGA+AGG - chr7_2:6740842-6740861 None:intergenic 45.0%
TATTGTGATGCCTGCTCAAC+CGG + chr7_2:6740927-6740946 Msa1046240:CDS 45.0%
! CAAGGTACTTGGTACATACC+TGG - chr7_2:6740981-6741000 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG - chr7_2:6741091-6741110 None:intergenic 45.0%
CAGTTAGTTCCATCCACTGA+TGG - chr7_2:6741388-6741407 None:intergenic 45.0%
AACTGCGAAATTGCCATGGA+TGG + chr7_2:6741403-6741422 Msa1046240:CDS 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG + chr7_2:6741496-6741515 Msa1046240:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG - chr7_2:6741502-6741521 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG - chr7_2:6741552-6741571 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG - chr7_2:6741661-6741680 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG - chr7_2:6741859-6741878 None:intergenic 45.0%
GGGTCCCCTAAAATAAAAGG+AGG - chr7_2:6741958-6741977 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG - chr7_2:6741961-6741980 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG - chr7_2:6741978-6741997 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG - chr7_2:6741980-6741999 None:intergenic 45.0%
ACAGCAGAGATGTCAGAAGA+AGG - chr7_2:6740842-6740861 None:intergenic 45.0%
TATTGTGATGCCTGCTCAAC+CGG + chr7_2:6740927-6740946 Msa1046240:CDS 45.0%
! CAAGGTACTTGGTACATACC+TGG - chr7_2:6740981-6741000 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG - chr7_2:6741091-6741110 None:intergenic 45.0%
CAGTTAGTTCCATCCACTGA+TGG - chr7_2:6741388-6741407 None:intergenic 45.0%
AACTGCGAAATTGCCATGGA+TGG + chr7_2:6741403-6741422 Msa1046240:CDS 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG + chr7_2:6741496-6741515 Msa1046240:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG - chr7_2:6741502-6741521 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG - chr7_2:6741552-6741571 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG - chr7_2:6741661-6741680 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG - chr7_2:6741859-6741878 None:intergenic 45.0%
GGGTCCCCTAAAATAAAAGG+AGG - chr7_2:6741958-6741977 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG - chr7_2:6741961-6741980 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG - chr7_2:6741978-6741997 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG - chr7_2:6741980-6741999 None:intergenic 45.0%
TGTGACAGTTGGAGTGTGTG+CGG - chr7_2:6740738-6740757 None:intergenic 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG + chr7_2:6741445-6741464 Msa1046240:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG + chr7_2:6741659-6741678 Msa1046240:three_prime_UTR 50.0%
GAGGTGATTGAGAGTGGTGT+AGG - chr7_2:6741939-6741958 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG + chr7_2:6741965-6741984 Msa1046240:three_prime_UTR 50.0%
GTCGAGAGAGAACACACAAG+AGG - chr7_2:6741994-6742013 None:intergenic 50.0%
TGTGACAGTTGGAGTGTGTG+CGG - chr7_2:6740738-6740757 None:intergenic 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG + chr7_2:6741445-6741464 Msa1046240:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG + chr7_2:6741659-6741678 Msa1046240:three_prime_UTR 50.0%
GAGGTGATTGAGAGTGGTGT+AGG - chr7_2:6741939-6741958 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG + chr7_2:6741965-6741984 Msa1046240:three_prime_UTR 50.0%
GTCGAGAGAGAACACACAAG+AGG - chr7_2:6741994-6742013 None:intergenic 50.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG - chr7_2:6740940-6740959 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG - chr7_2:6741653-6741672 None:intergenic 55.0%
CAGCTGCTAGCTAGCTAGCT+AGG - chr7_2:6742100-6742119 None:intergenic 55.0%
GTTGGAGTGTGTGCGGTTCA+CGG - chr7_2:6740731-6740750 None:intergenic 55.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG - chr7_2:6740940-6740959 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG - chr7_2:6741653-6741672 None:intergenic 55.0%
CAGCTGCTAGCTAGCTAGCT+AGG - chr7_2:6742100-6742119 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 6740731 6742250 6740731 ID=Msa1046240;Name=Msa1046240
chr7_2 mRNA 6740731 6742250 6740731 ID=Msa1046240-mRNA-1;Parent=Msa1046240;Name=Msa1046240-mRNA-1;_AED=0.01;_eAED=0.01;_QI=88|1|1|1|1|1|2|623|173
chr7_2 exon 6740731 6740981 6740731 ID=Msa1046240-mRNA-1:exon:1543;Parent=Msa1046240-mRNA-1
chr7_2 exon 6741269 6742250 6741269 ID=Msa1046240-mRNA-1:exon:1544;Parent=Msa1046240-mRNA-1
chr7_2 five_prime_UTR 6740731 6740818 6740731 ID=Msa1046240-mRNA-1:five_prime_utr;Parent=Msa1046240-mRNA-1
chr7_2 CDS 6740819 6740981 6740819 ID=Msa1046240-mRNA-1:cds;Parent=Msa1046240-mRNA-1
chr7_2 CDS 6741269 6741627 6741269 ID=Msa1046240-mRNA-1:cds;Parent=Msa1046240-mRNA-1
chr7_2 three_prime_UTR 6741628 6742250 6741628 ID=Msa1046240-mRNA-1:three_prime_utr;Parent=Msa1046240-mRNA-1
Gene Sequence

>Msa1046240

ATGGATCCAATTGGTTTCTTCCTTCTTCTGACATCTCTGCTGTTCAGTTATCCATTTGTTCTCATTGCTCAATCTCCCACAACTACTATCAGTGTTGAGGGAATTGTTTATTGTGATGCCTGCTCAACCGGCACTTTCTCTAAACACAGTTATCTCTTGCCAGGTGTTGACGTTCATATAGAGTGCAGATTTAGAGCAAGCTCACCAAGAACCAACGAACAGATAAATTTCTCAGTGAATAGAACAACAGACAGAGAGGGAGCATACAAATTAAATATACCATCAGTGGATGGAACTAACTGCGAAATTGCCATGGATGGTAATTCACAGATTGTGACTCTCTGCGAAGCAAGCTTGATAGGAACCTCATCATCTTCTTATTCTTGCAATGTTCCTTTCTTGAAGAGCACAGGAAGTAGTGAGATATCAAAACAAGAGAATAACCTATGTGTATACAGCTTGGGAGCTCTTAGTTACAAGCCACCTGAAAATATGATCAATACTACCTTATGCTCAAATTAA
Protein sequence

>Msa1046240

MDPIGFFLLLTSLLFSYPFVLIAQSPTTTISVEGIVYCDACSTGTFSKHSYLLPGVDVHIECRFRASSPRTNEQINFSVNRTTDREGAYKLNIPSVDGTNCEIAMDGNSQIVTLCEASLIGTSSSSYSCNVPFLKSTGSSEISKQENNLCVYSLGALSYKPPENMINTTLCSN*