AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1046270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1046270 MtrunA17_Chr7g0214211 95.954 173 7 0 1 173 1 173 3.96e-113 318
Msa1046270 MtrunA17_Chr7g0224361 46.309 149 71 5 26 173 33 173 1.51e-41 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1046270 AT5G13140.1 50.667 150 66 5 26 173 35 178 5.75e-44 146
Msa1046270 AT5G41050.1 46.939 147 72 4 26 171 29 170 1.18e-37 127
Msa1046270 AT3G26960.1 43.478 138 69 3 26 161 33 163 4.39e-32 113

Find 73 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACCCCTTCCCTTAAATT+TGG 0.165885 7_2:-6764396 Msa1046270:three_prime_UTR
AGAAAATGAAGAAAGGATTT+TGG 0.210001 7_2:+6764236 None:intergenic
ATCACCCAATCCCAAATTTA+AGG 0.239476 7_2:+6764385 None:intergenic
TGATAGATGTAATTCATAAA+AGG 0.239602 7_2:+6764129 None:intergenic
AGTGGCGTGCATCGGACTTT+TGG 0.261878 7_2:+6764618 None:intergenic
ATTGTCCACTTTAGATATTA+TGG 0.289337 7_2:-6764585 Msa1046270:three_prime_UTR
TATGCTCAAATTAATTCATT+TGG 0.298493 7_2:-6764654 Msa1046270:exon
GGACCCCTTCCCTTAAATTT+GGG 0.298950 7_2:-6764395 Msa1046270:three_prime_UTR
ATTGGATCCATGTGGAATAT+TGG 0.305436 7_2:+6765461 None:intergenic
AGGTGCTATAGAATGAATTT+TGG 0.319346 7_2:+6764432 None:intergenic
TTTATCTGTTCGTTGGTTCT+TGG 0.331613 7_2:+6764958 None:intergenic
GTGGGGTCCCCTAAAATAAA+AGG 0.343139 7_2:+6764310 None:intergenic
GGACATATCTATAAACAAAA+TGG 0.345515 7_2:+6764037 None:intergenic
CCTTCCCTTAAATTTGGGAT+TGG 0.346089 7_2:-6764390 Msa1046270:three_prime_UTR
AATGAATTAATTTGAGCATA+AGG 0.365031 7_2:+6764657 None:intergenic
CTTCCCTTAAATTTGGGATT+GGG 0.381813 7_2:-6764389 Msa1046270:three_prime_UTR
GGATAAGGATATTGAGTAAG+AGG 0.392047 7_2:+6764353 None:intergenic
TATTGTGATGCCTGCTCAAC+CGG 0.409183 7_2:-6765341 Msa1046270:CDS
CTCTGCGAAGCAAGCTTGAT+AGG 0.425118 7_2:-6764823 Msa1046270:CDS
CTCAACACTGATAGTGGTTG+TGG 0.425792 7_2:+6765373 None:intergenic
ATTGAGAGTGGTGTAGGATA+AGG 0.427502 7_2:+6764338 None:intergenic
GATTGAGCAATGAGAACAAA+TGG 0.438625 7_2:+6765398 None:intergenic
AGAGAAAGTGCCGGTTGAGC+AGG 0.454772 7_2:+6765331 None:intergenic
AGAAGAAGGAAGAAACCAAT+TGG 0.454824 7_2:+6765443 None:intergenic
GAGGTGATTGAGAGTGGTGT+AGG 0.457528 7_2:+6764332 None:intergenic
AATTCCCTCAACACTGATAG+TGG 0.459627 7_2:+6765367 None:intergenic
GATAGATGTAATTCATAAAA+GGG 0.468871 7_2:+6764130 None:intergenic
AACCTATGTGTATACAGCTT+GGG 0.470069 7_2:-6764721 Msa1046270:CDS
TCACCCAATCCCAAATTTAA+GGG 0.471045 7_2:+6764386 None:intergenic
ATATTCCACATGGATCCAAT+TGG 0.475963 7_2:-6765458 Msa1046270:exon
CAGCTGCTAGCTAGCTAGCT+AGG 0.480010 7_2:+6764167 None:intergenic
TGAGAAATTTATCTGTTCGT+TGG 0.493906 7_2:+6764951 None:intergenic
TATTGAGTAAGAGGAAGAGA+AGG 0.503270 7_2:+6764362 None:intergenic
CTTCCTGTGCTCTTCAAGAA+AGG 0.504211 7_2:+6764769 None:intergenic
CACACAACAATATATACATA+TGG 0.507745 7_2:-6765514 Msa1046270:five_prime_UTR
GTTCCTTTCTTGAAGAGCAC+AGG 0.511194 7_2:-6764772 Msa1046270:CDS
TAACCTATGTGTATACAGCT+TGG 0.520473 7_2:-6764722 Msa1046270:CDS
AGTTTCCATAATATCTAAAG+TGG 0.521030 7_2:+6764580 None:intergenic
ACAACCACTATCAGTGTTGA+GGG 0.524635 7_2:-6765371 Msa1046270:CDS
AAACACAGTTATCTCTTGCC+AGG 0.531682 7_2:-6765308 Msa1046270:intron
CAATCCCAAATTTAAGGGAA+GGG 0.535979 7_2:+6764391 None:intergenic
AAAGTTGTTGTTGAATTGAG+TGG 0.538831 7_2:-6764535 Msa1046270:three_prime_UTR
ACAAGAGGAATCCAAGTTGT+GGG 0.540830 7_2:+6764292 None:intergenic
CAGTTAGTTCCATCCACTGA+TGG 0.541304 7_2:+6764883 None:intergenic
AATCCCAAATTTAAGGGAAG+GGG 0.555629 7_2:+6764392 None:intergenic
CCAATCCCAAATTTAAGGGA+AGG 0.556433 7_2:+6764390 None:intergenic
CTTAGAAAAGTGGCGTGCAT+CGG 0.557367 7_2:+6764610 None:intergenic
CAAATTAAATATACCATCAG+TGG 0.559042 7_2:-6764896 Msa1046270:CDS
CACAAGAGGAATCCAAGTTG+TGG 0.568323 7_2:+6764291 None:intergenic
CACAACCACTATCAGTGTTG+AGG 0.573227 7_2:-6765372 Msa1046270:CDS
CTTGATTCCAATATTCCACA+TGG 0.573944 7_2:-6765468 Msa1046270:exon
AACTGCGAAATTGCCATGGA+TGG 0.574094 7_2:-6764865 Msa1046270:CDS
TCAACACTGATAGTGGTTGT+GGG 0.576625 7_2:+6765374 None:intergenic
AATCTGTGAATTACCATCCA+TGG 0.577346 7_2:+6764852 None:intergenic
GGGTCCAAGGAAAGAAAAGA+AGG 0.578723 7_2:+6764412 None:intergenic
ACAGCAGAGATGTCAGAAGA+AGG 0.579115 7_2:+6765429 None:intergenic
AATTTAAGGGAAGGGGTCCA+AGG 0.579853 7_2:+6764399 None:intergenic
ATATATTGTTGTGTGACAGT+TGG 0.583761 7_2:+6765522 None:intergenic
TGGACAATACCTTAGAAAAG+TGG 0.588227 7_2:+6764600 None:intergenic
TAAAAGGAGGTGATTGAGAG+TGG 0.589362 7_2:+6764326 None:intergenic
AACTAACTGCGAAATTGCCA+TGG 0.592115 7_2:-6764869 Msa1046270:CDS
TTCACATTACTTGAAAATCG+AGG 0.594081 7_2:+6764483 None:intergenic
TTAAATATACCATCAGTGGA+TGG 0.597816 7_2:-6764892 Msa1046270:CDS
AAGTTGTTGTTGAATTGAGT+GGG 0.604852 7_2:-6764534 Msa1046270:three_prime_UTR
AGAGAGAGAGAACACACAAG+AGG 0.619901 7_2:+6764277 None:intergenic
GCAAGAATAAGAAGATGATG+AGG 0.636237 7_2:+6764798 None:intergenic
GAATAGAACAACAGACAGAG+AGG 0.636780 7_2:-6764926 Msa1046270:CDS
CTCCCAAGCTGTATACACAT+AGG 0.650090 7_2:+6764719 None:intergenic
AATAGAACAACAGACAGAGA+GGG 0.660031 7_2:-6764925 Msa1046270:CDS
ACTGTGTTTAGAGAAAGTGC+CGG 0.671971 7_2:+6765322 None:intergenic
GGGTCCCCTAAAATAAAAGG+AGG 0.676821 7_2:+6764313 None:intergenic
CAAGAGGAATCCAAGTTGTG+GGG 0.691066 7_2:+6764293 None:intergenic
AGAAACCAATTGGATCCATG+TGG 0.695775 7_2:+6765453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCATTTAATTATACATATAA+TGG + chr7_2:6764332-6764351 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG + chr7_2:6765347-6765366 None:intergenic 15.0%
!! GCATTTAATTATACATATAA+TGG + chr7_2:6764332-6764351 None:intergenic 15.0%
!! AATAAAAAGAAAATGAAGAA+AGG + chr7_2:6765347-6765366 None:intergenic 15.0%
!! TATCTATGATGATATATATC+AGG - chr7_2:6764552-6764571 Msa1046270:three_prime_UTR 20.0%
!!! AATGAATTAATTTGAGCATA+AGG + chr7_2:6764919-6764938 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG - chr7_2:6764919-6764938 Msa1046270:CDS 20.0%
!!! TCAAGTAATGTGAAATTTTT+TGG - chr7_2:6765099-6765118 Msa1046270:intron 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG + chr7_2:6765129-6765148 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG + chr7_2:6765446-6765465 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG + chr7_2:6765447-6765466 None:intergenic 20.0%
!! TATCTATGATGATATATATC+AGG - chr7_2:6764552-6764571 Msa1046270:three_prime_UTR 20.0%
!!! AATGAATTAATTTGAGCATA+AGG + chr7_2:6764919-6764938 None:intergenic 20.0%
!! TATGCTCAAATTAATTCATT+TGG - chr7_2:6764919-6764938 Msa1046270:CDS 20.0%
!!! TCAAGTAATGTGAAATTTTT+TGG - chr7_2:6765099-6765118 Msa1046270:intron 20.0%
!!! AATTTTGGCTAATTTTGTTT+TGG + chr7_2:6765129-6765148 None:intergenic 20.0%
!! GATAGATGTAATTCATAAAA+GGG + chr7_2:6765446-6765465 None:intergenic 20.0%
!! TGATAGATGTAATTCATAAA+AGG + chr7_2:6765447-6765466 None:intergenic 20.0%
! CACACAACAATATATACATA+TGG - chr7_2:6764059-6764078 Msa1046270:three_prime_UTR 25.0%
! CAAATTAAATATACCATCAG+TGG - chr7_2:6764677-6764696 Msa1046270:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG + chr7_2:6764897-6764916 None:intergenic 25.0%
! ATTGTCCACTTTAGATATTA+TGG - chr7_2:6764988-6765007 Msa1046270:CDS 25.0%
! AGTTTCCATAATATCTAAAG+TGG + chr7_2:6764996-6765015 None:intergenic 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG - chr7_2:6765255-6765274 Msa1046270:intron 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG + chr7_2:6765340-6765359 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG + chr7_2:6765539-6765558 None:intergenic 25.0%
! CACACAACAATATATACATA+TGG - chr7_2:6764059-6764078 Msa1046270:three_prime_UTR 25.0%
! CAAATTAAATATACCATCAG+TGG - chr7_2:6764677-6764696 Msa1046270:CDS 25.0%
!!! GTAGTATTGATCATATTTTC+AGG + chr7_2:6764897-6764916 None:intergenic 25.0%
! ATTGTCCACTTTAGATATTA+TGG - chr7_2:6764988-6765007 Msa1046270:CDS 25.0%
! AGTTTCCATAATATCTAAAG+TGG + chr7_2:6764996-6765015 None:intergenic 25.0%
!!! AATCACCTCCTTTTATTTTA+GGG - chr7_2:6765255-6765274 Msa1046270:intron 25.0%
!! AGAAAATGAAGAAAGGATTT+TGG + chr7_2:6765340-6765359 None:intergenic 25.0%
! GGACATATCTATAAACAAAA+TGG + chr7_2:6765539-6765558 None:intergenic 25.0%
ATATATTGTTGTGTGACAGT+TGG + chr7_2:6764054-6764073 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG + chr7_2:6764304-6764323 None:intergenic 30.0%
TGAGAAATTTATCTGTTCGT+TGG + chr7_2:6764625-6764644 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG - chr7_2:6764681-6764700 Msa1046270:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG + chr7_2:6764894-6764913 None:intergenic 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG - chr7_2:6765038-6765057 Msa1046270:intron 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG - chr7_2:6765039-6765058 Msa1046270:intron 30.0%
TTCACATTACTTGAAAATCG+AGG + chr7_2:6765093-6765112 None:intergenic 30.0%
!! AGGTGCTATAGAATGAATTT+TGG + chr7_2:6765144-6765163 None:intergenic 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG - chr7_2:6765254-6765273 Msa1046270:intron 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG - chr7_2:6765256-6765275 Msa1046270:intron 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG + chr7_2:6765334-6765353 None:intergenic 30.0%
ATATATTGTTGTGTGACAGT+TGG + chr7_2:6764054-6764073 None:intergenic 30.0%
ATATTGCATGAGAAAGAACA+AGG + chr7_2:6764304-6764323 None:intergenic 30.0%
TGAGAAATTTATCTGTTCGT+TGG + chr7_2:6764625-6764644 None:intergenic 30.0%
TTAAATATACCATCAGTGGA+TGG - chr7_2:6764681-6764700 Msa1046270:CDS 30.0%
!! GTATTGATCATATTTTCAGG+TGG + chr7_2:6764894-6764913 None:intergenic 30.0%
! AAAGTTGTTGTTGAATTGAG+TGG - chr7_2:6765038-6765057 Msa1046270:intron 30.0%
! AAGTTGTTGTTGAATTGAGT+GGG - chr7_2:6765039-6765058 Msa1046270:intron 30.0%
TTCACATTACTTGAAAATCG+AGG + chr7_2:6765093-6765112 None:intergenic 30.0%
!! AGGTGCTATAGAATGAATTT+TGG + chr7_2:6765144-6765163 None:intergenic 30.0%
!!! CAATCACCTCCTTTTATTTT+AGG - chr7_2:6765254-6765273 Msa1046270:intron 30.0%
!!! ATCACCTCCTTTTATTTTAG+GGG - chr7_2:6765256-6765275 Msa1046270:intron 30.0%
!!! TGAAGAAAGGATTTTGGTTT+AGG + chr7_2:6765334-6765353 None:intergenic 30.0%
CTTGATTCCAATATTCCACA+TGG - chr7_2:6764105-6764124 Msa1046270:three_prime_UTR 35.0%
ATTGGATCCATGTGGAATAT+TGG + chr7_2:6764115-6764134 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG - chr7_2:6764115-6764134 Msa1046270:three_prime_UTR 35.0%
AGAAGAAGGAAGAAACCAAT+TGG + chr7_2:6764133-6764152 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG + chr7_2:6764178-6764197 None:intergenic 35.0%
! ATGAGAAAGAACAAGGTACT+TGG + chr7_2:6764297-6764316 None:intergenic 35.0%
! GCTATTTAGCGCTGAATTAA+AGG - chr7_2:6764350-6764369 Msa1046270:three_prime_UTR 35.0%
ATGTATGGTGTATGAGATAC+TGG + chr7_2:6764381-6764400 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG - chr7_2:6764383-6764402 Msa1046270:three_prime_UTR 35.0%
AGTGTTTATGTGTCAGTGTT+TGG - chr7_2:6764470-6764489 Msa1046270:three_prime_UTR 35.0%
GCGTCTATGTCTTAACTTTA+TGG - chr7_2:6764492-6764511 Msa1046270:three_prime_UTR 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG + chr7_2:6764618-6764637 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG - chr7_2:6764648-6764667 Msa1046270:exon 35.0%
AATCTGTGAATTACCATCCA+TGG + chr7_2:6764724-6764743 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG + chr7_2:6764778-6764797 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG - chr7_2:6764851-6764870 Msa1046270:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG - chr7_2:6764852-6764871 Msa1046270:CDS 35.0%
TGGACAATACCTTAGAAAAG+TGG + chr7_2:6764976-6764995 None:intergenic 35.0%
AATCCCAAATTTAAGGGAAG+GGG + chr7_2:6765184-6765203 None:intergenic 35.0%
CAATCCCAAATTTAAGGGAA+GGG + chr7_2:6765185-6765204 None:intergenic 35.0%
! CTTCCCTTAAATTTGGGATT+GGG - chr7_2:6765184-6765203 Msa1046270:intron 35.0%
TCACCCAATCCCAAATTTAA+GGG + chr7_2:6765190-6765209 None:intergenic 35.0%
ATCACCCAATCCCAAATTTA+AGG + chr7_2:6765191-6765210 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG + chr7_2:6765214-6765233 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG + chr7_2:6765223-6765242 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG + chr7_2:6765331-6765350 None:intergenic 35.0%
CTTGATTCCAATATTCCACA+TGG - chr7_2:6764105-6764124 Msa1046270:three_prime_UTR 35.0%
ATTGGATCCATGTGGAATAT+TGG + chr7_2:6764115-6764134 None:intergenic 35.0%
ATATTCCACATGGATCCAAT+TGG - chr7_2:6764115-6764134 Msa1046270:three_prime_UTR 35.0%
AGAAGAAGGAAGAAACCAAT+TGG + chr7_2:6764133-6764152 None:intergenic 35.0%
GATTGAGCAATGAGAACAAA+TGG + chr7_2:6764178-6764197 None:intergenic 35.0%
! ATGAGAAAGAACAAGGTACT+TGG + chr7_2:6764297-6764316 None:intergenic 35.0%
! GCTATTTAGCGCTGAATTAA+AGG - chr7_2:6764350-6764369 Msa1046270:three_prime_UTR 35.0%
ATGTATGGTGTATGAGATAC+TGG + chr7_2:6764381-6764400 None:intergenic 35.0%
ATCTCATACACCATACATGT+TGG - chr7_2:6764383-6764402 Msa1046270:three_prime_UTR 35.0%
AGTGTTTATGTGTCAGTGTT+TGG - chr7_2:6764470-6764489 Msa1046270:three_prime_UTR 35.0%
GCGTCTATGTCTTAACTTTA+TGG - chr7_2:6764492-6764511 Msa1046270:three_prime_UTR 35.0%
!! TTTATCTGTTCGTTGGTTCT+TGG + chr7_2:6764618-6764637 None:intergenic 35.0%
AATAGAACAACAGACAGAGA+GGG - chr7_2:6764648-6764667 Msa1046270:exon 35.0%
AATCTGTGAATTACCATCCA+TGG + chr7_2:6764724-6764743 None:intergenic 35.0%
GCAAGAATAAGAAGATGATG+AGG + chr7_2:6764778-6764797 None:intergenic 35.0%
TAACCTATGTGTATACAGCT+TGG - chr7_2:6764851-6764870 Msa1046270:CDS 35.0%
AACCTATGTGTATACAGCTT+GGG - chr7_2:6764852-6764871 Msa1046270:CDS 35.0%
TGGACAATACCTTAGAAAAG+TGG + chr7_2:6764976-6764995 None:intergenic 35.0%
AATCCCAAATTTAAGGGAAG+GGG + chr7_2:6765184-6765203 None:intergenic 35.0%
CAATCCCAAATTTAAGGGAA+GGG + chr7_2:6765185-6765204 None:intergenic 35.0%
! CTTCCCTTAAATTTGGGATT+GGG - chr7_2:6765184-6765203 Msa1046270:intron 35.0%
TCACCCAATCCCAAATTTAA+GGG + chr7_2:6765190-6765209 None:intergenic 35.0%
ATCACCCAATCCCAAATTTA+AGG + chr7_2:6765191-6765210 None:intergenic 35.0%
TATTGAGTAAGAGGAAGAGA+AGG + chr7_2:6765214-6765233 None:intergenic 35.0%
GGATAAGGATATTGAGTAAG+AGG + chr7_2:6765223-6765242 None:intergenic 35.0%
!!! AGAAAGGATTTTGGTTTAGG+AGG + chr7_2:6765331-6765350 None:intergenic 35.0%
AGAAACCAATTGGATCCATG+TGG + chr7_2:6764123-6764142 None:intergenic 40.0%
!! TCAACACTGATAGTGGTTGT+GGG + chr7_2:6764202-6764221 None:intergenic 40.0%
! ACAACCACTATCAGTGTTGA+GGG - chr7_2:6764202-6764221 Msa1046270:three_prime_UTR 40.0%
AATTCCCTCAACACTGATAG+TGG + chr7_2:6764209-6764228 None:intergenic 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG + chr7_2:6764254-6764273 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG - chr7_2:6764265-6764284 Msa1046270:three_prime_UTR 40.0%
GAATAGAACAACAGACAGAG+AGG - chr7_2:6764647-6764666 Msa1046270:exon 40.0%
AACTAACTGCGAAATTGCCA+TGG - chr7_2:6764704-6764723 Msa1046270:CDS 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG - chr7_2:6765157-6765176 Msa1046270:intron 40.0%
CCAATCCCAAATTTAAGGGA+AGG + chr7_2:6765186-6765205 None:intergenic 40.0%
! CCTTCCCTTAAATTTGGGAT+TGG - chr7_2:6765183-6765202 Msa1046270:intron 40.0%
ATTGAGAGTGGTGTAGGATA+AGG + chr7_2:6765238-6765257 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG + chr7_2:6765250-6765269 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG + chr7_2:6765284-6765303 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG + chr7_2:6765325-6765344 None:intergenic 40.0%
AGAAACCAATTGGATCCATG+TGG + chr7_2:6764123-6764142 None:intergenic 40.0%
!! TCAACACTGATAGTGGTTGT+GGG + chr7_2:6764202-6764221 None:intergenic 40.0%
! ACAACCACTATCAGTGTTGA+GGG - chr7_2:6764202-6764221 Msa1046270:three_prime_UTR 40.0%
AATTCCCTCAACACTGATAG+TGG + chr7_2:6764209-6764228 None:intergenic 40.0%
! ACTGTGTTTAGAGAAAGTGC+CGG + chr7_2:6764254-6764273 None:intergenic 40.0%
AAACACAGTTATCTCTTGCC+AGG - chr7_2:6764265-6764284 Msa1046270:three_prime_UTR 40.0%
GAATAGAACAACAGACAGAG+AGG - chr7_2:6764647-6764666 Msa1046270:exon 40.0%
AACTAACTGCGAAATTGCCA+TGG - chr7_2:6764704-6764723 Msa1046270:CDS 40.0%
! AGCACCTTCTTTTCTTTCCT+TGG - chr7_2:6765157-6765176 Msa1046270:intron 40.0%
CCAATCCCAAATTTAAGGGA+AGG + chr7_2:6765186-6765205 None:intergenic 40.0%
! CCTTCCCTTAAATTTGGGAT+TGG - chr7_2:6765183-6765202 Msa1046270:intron 40.0%
ATTGAGAGTGGTGTAGGATA+AGG + chr7_2:6765238-6765257 None:intergenic 40.0%
TAAAAGGAGGTGATTGAGAG+TGG + chr7_2:6765250-6765269 None:intergenic 40.0%
ACAAGAGGAATCCAAGTTGT+GGG + chr7_2:6765284-6765303 None:intergenic 40.0%
!!! GATTTTGGTTTAGGAGGTGA+TGG + chr7_2:6765325-6765344 None:intergenic 40.0%
ACAGCAGAGATGTCAGAAGA+AGG + chr7_2:6764147-6764166 None:intergenic 45.0%
!! CTCAACACTGATAGTGGTTG+TGG + chr7_2:6764203-6764222 None:intergenic 45.0%
CACAACCACTATCAGTGTTG+AGG - chr7_2:6764201-6764220 Msa1046270:three_prime_UTR 45.0%
TATTGTGATGCCTGCTCAAC+CGG - chr7_2:6764232-6764251 Msa1046270:three_prime_UTR 45.0%
! CAAGGTACTTGGTACATACC+TGG + chr7_2:6764286-6764305 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG + chr7_2:6764396-6764415 None:intergenic 45.0%
TGACACGATAACGAGACACA+TGG - chr7_2:6764410-6764429 Msa1046270:three_prime_UTR 45.0%
CAGTTAGTTCCATCCACTGA+TGG + chr7_2:6764693-6764712 None:intergenic 45.0%
AACTGCGAAATTGCCATGGA+TGG - chr7_2:6764708-6764727 Msa1046270:CDS 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG - chr7_2:6764801-6764820 Msa1046270:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG + chr7_2:6764807-6764826 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG + chr7_2:6764857-6764876 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG + chr7_2:6764966-6764985 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG + chr7_2:6765164-6765183 None:intergenic 45.0%
AATTTAAGGGAAGGGGTCCA+AGG + chr7_2:6765177-6765196 None:intergenic 45.0%
TGGACCCCTTCCCTTAAATT+TGG - chr7_2:6765177-6765196 Msa1046270:intron 45.0%
GGACCCCTTCCCTTAAATTT+GGG - chr7_2:6765178-6765197 Msa1046270:intron 45.0%
GGGTCCCCTAAAATAAAAGG+AGG + chr7_2:6765263-6765282 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG + chr7_2:6765266-6765285 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG + chr7_2:6765283-6765302 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG + chr7_2:6765285-6765304 None:intergenic 45.0%
AGAGAGAGAGAACACACAAG+AGG + chr7_2:6765299-6765318 None:intergenic 45.0%
ACAGCAGAGATGTCAGAAGA+AGG + chr7_2:6764147-6764166 None:intergenic 45.0%
!! CTCAACACTGATAGTGGTTG+TGG + chr7_2:6764203-6764222 None:intergenic 45.0%
CACAACCACTATCAGTGTTG+AGG - chr7_2:6764201-6764220 Msa1046270:three_prime_UTR 45.0%
TATTGTGATGCCTGCTCAAC+CGG - chr7_2:6764232-6764251 Msa1046270:three_prime_UTR 45.0%
! CAAGGTACTTGGTACATACC+TGG + chr7_2:6764286-6764305 None:intergenic 45.0%
GTGTCAGACACCAACATGTA+TGG + chr7_2:6764396-6764415 None:intergenic 45.0%
TGACACGATAACGAGACACA+TGG - chr7_2:6764410-6764429 Msa1046270:three_prime_UTR 45.0%
CAGTTAGTTCCATCCACTGA+TGG + chr7_2:6764693-6764712 None:intergenic 45.0%
AACTGCGAAATTGCCATGGA+TGG - chr7_2:6764708-6764727 Msa1046270:CDS 45.0%
! GTTCCTTTCTTGAAGAGCAC+AGG - chr7_2:6764801-6764820 Msa1046270:CDS 45.0%
CTTCCTGTGCTCTTCAAGAA+AGG + chr7_2:6764807-6764826 None:intergenic 45.0%
CTCCCAAGCTGTATACACAT+AGG + chr7_2:6764857-6764876 None:intergenic 45.0%
CTTAGAAAAGTGGCGTGCAT+CGG + chr7_2:6764966-6764985 None:intergenic 45.0%
GGGTCCAAGGAAAGAAAAGA+AGG + chr7_2:6765164-6765183 None:intergenic 45.0%
AATTTAAGGGAAGGGGTCCA+AGG + chr7_2:6765177-6765196 None:intergenic 45.0%
TGGACCCCTTCCCTTAAATT+TGG - chr7_2:6765177-6765196 Msa1046270:intron 45.0%
GGACCCCTTCCCTTAAATTT+GGG - chr7_2:6765178-6765197 Msa1046270:intron 45.0%
GGGTCCCCTAAAATAAAAGG+AGG + chr7_2:6765263-6765282 None:intergenic 45.0%
GTGGGGTCCCCTAAAATAAA+AGG + chr7_2:6765266-6765285 None:intergenic 45.0%
CAAGAGGAATCCAAGTTGTG+GGG + chr7_2:6765283-6765302 None:intergenic 45.0%
CACAAGAGGAATCCAAGTTG+TGG + chr7_2:6765285-6765304 None:intergenic 45.0%
AGAGAGAGAGAACACACAAG+AGG + chr7_2:6765299-6765318 None:intergenic 45.0%
CTCTGCGAAGCAAGCTTGAT+AGG - chr7_2:6764750-6764769 Msa1046270:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG - chr7_2:6764964-6764983 Msa1046270:CDS 50.0%
GAGGTGATTGAGAGTGGTGT+AGG + chr7_2:6765244-6765263 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG - chr7_2:6765270-6765289 Msa1046270:intron 50.0%
CTCTGCGAAGCAAGCTTGAT+AGG - chr7_2:6764750-6764769 Msa1046270:CDS 50.0%
!!! CGATGCACGCCACTTTTCTA+AGG - chr7_2:6764964-6764983 Msa1046270:CDS 50.0%
GAGGTGATTGAGAGTGGTGT+AGG + chr7_2:6765244-6765263 None:intergenic 50.0%
!! TTTTAGGGGACCCCACAACT+TGG - chr7_2:6765270-6765289 Msa1046270:intron 50.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG + chr7_2:6764245-6764264 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG + chr7_2:6764958-6764977 None:intergenic 55.0%
CAGCTGCTAGCTAGCTAGCT+AGG + chr7_2:6765409-6765428 None:intergenic 55.0%
CAGTTGGAGTGTGTGCGTTC+AGG + chr7_2:6764038-6764057 None:intergenic 55.0%
!! AGAGAAAGTGCCGGTTGAGC+AGG + chr7_2:6764245-6764264 None:intergenic 55.0%
! AGTGGCGTGCATCGGACTTT+TGG + chr7_2:6764958-6764977 None:intergenic 55.0%
CAGCTGCTAGCTAGCTAGCT+AGG + chr7_2:6765409-6765428 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 6764037 6765558 6764037 ID=Msa1046270;Name=Msa1046270
chr7_2 mRNA 6764037 6765558 6764037 ID=Msa1046270-mRNA-1;Parent=Msa1046270;Name=Msa1046270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=87|1|1|1|1|1|2|626|173
chr7_2 exon 6764037 6765021 6764037 ID=Msa1046270-mRNA-1:exon:1567;Parent=Msa1046270-mRNA-1
chr7_2 exon 6765309 6765558 6765309 ID=Msa1046270-mRNA-1:exon:1566;Parent=Msa1046270-mRNA-1
chr7_2 five_prime_UTR 6765472 6765558 6765472 ID=Msa1046270-mRNA-1:five_prime_utr;Parent=Msa1046270-mRNA-1
chr7_2 CDS 6765309 6765471 6765309 ID=Msa1046270-mRNA-1:cds;Parent=Msa1046270-mRNA-1
chr7_2 CDS 6764663 6765021 6764663 ID=Msa1046270-mRNA-1:cds;Parent=Msa1046270-mRNA-1
chr7_2 three_prime_UTR 6764037 6764662 6764037 ID=Msa1046270-mRNA-1:three_prime_utr;Parent=Msa1046270-mRNA-1
Gene Sequence

>Msa1046270

ATGGATCCAATTGGTTTCTTCCTTCTTCTGACATCTCTGCTGTTCAGTTATCCATTTGTTCTCATTGCTCAATCTCCCACAACCACTATCAGTGTTGAGGGAATTGTTTATTGTGATGCCTGCTCAACCGGCACTTTCTCTAAACACAGTTATCTCTTGCCAGGTGTTGACGTTCATATAGAGTGCAGATTTAGAGCAAGCTCACCAAGAACCAACGAACAGATAAATTTCTCAGTGAATAGAACAACAGACAGAGAGGGAGCATACAAATTAAATATACCATCAGTGGATGGAACTAACTGCGAAATTGCCATGGATGGTAATTCACAGATTGTGACTCTCTGCGAAGCAAGCTTGATAGGAACCTCATCATCTTCTTATTCTTGCAATGTTCCTTTCTTGAAGAGCACAGGAAGTAGTGAGATATCAAAACAAGAGAATAACCTATGTGTATACAGCTTGGGAGCTCTTAGTTACAAGCCACCTGAAAATATGATCAATACTACCTTATGCTCAAATTAA
Protein sequence

>Msa1046270

MDPIGFFLLLTSLLFSYPFVLIAQSPTTTISVEGIVYCDACSTGTFSKHSYLLPGVDVHIECRFRASSPRTNEQINFSVNRTTDREGAYKLNIPSVDGTNCEIAMDGNSQIVTLCEASLIGTSSSSYSCNVPFLKSTGSSEISKQENNLCVYSLGALSYKPPENMINTTLCSN*