AlfalfaGEDB | Msa1006620

M. sativa cultivar zhongmu-4 / Msa1006620

KEGG: K04733
GO: -
PFAM: PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006620	AES77384.1	64.925	804	196	6	1	732	1	790	0.0	976

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006620	sp\|Q8VZG8\|MIK2_ARATH	41.897	580	314	11	130	700	479	1044	1.02e-134	426
Msa1006620	sp\|Q8VZG8\|MIK2_ARATH	33.688	282	174	6	57	331	74	349	9.83e-23	107
Msa1006620	sp\|Q8VZG8\|MIK2_ARATH	33.190	232	146	4	106	330	239	468	2.02e-22	107
Msa1006620	sp\|Q8VZG8\|MIK2_ARATH	34.389	221	138	3	117	331	202	421	1.14e-20	101
Msa1006620	sp\|Q8VZG8\|MIK2_ARATH	30.435	253	147	4	107	330	360	612	3.63e-17	90.1
Msa1006620	sp\|Q8VZG8\|MIK2_ARATH	27.597	308	141	8	102	328	256	562	3.66e-16	86.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006620	G7KZX2	64.925	804	196	6	1	732	1	790	0.0	976

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0533430	Msa1006620	0.805202	1.496094e-49	-8.615850e-47
Msa0629940	Msa1006620	0.804187	2.436860e-49	-8.615850e-47
Msa0818010	Msa1006620	0.807764	4.309458e-50	-8.615850e-47
Msa0912370	Msa1006620	0.800736	1.252991e-48	-8.615850e-47
Msa1006250	Msa1006620	0.843855	1.084151e-58	-8.615850e-47
Msa1006590	Msa1006620	0.941305	5.237135e-101	-8.615850e-47
Msa1006620	Msa1136010	0.831345	1.759443e-55	-8.615850e-47
Msa1006620	Msa1289490	0.807303	5.398146e-50	-8.615850e-47
Msa1006620	Msa1359820	0.802072	6.673710e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1006620	MtrunA17_Chr7g0217061	64.925	804	196	6	1	732	1	790	976
Msa1006620	MtrunA17_Chr7g0218991	64.644	758	239	4	1	732	9	763	963
Msa1006620	MtrunA17_Chr7g0215641	63.694	785	208	7	16	733	1	775	932
Msa1006620	MtrunA17_Chr7g0215061	60.000	825	226	11	10	732	6	828	916
Msa1006620	MtrunA17_Chr7g0215671	63.613	764	238	8	1	732	1	756	912
Msa1006620	MtrunA17_Chr7g0215631	65.513	751	223	7	16	732	1	749	910
Msa1006620	MtrunA17_Chr7g0217081	57.548	881	196	10	16	732	1	867	888
Msa1006620	MtrunA17_Chr7g0215651	62.550	745	246	8	16	732	1	740	886
Msa1006620	MtrunA17_Chr7g0215681	61.237	792	242	8	1	732	1	787	886
Msa1006620	MtrunA17_Chr7g0215011	61.298	801	223	10	1	730	1	785	882

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1006620	AT4G08850.1	41.897	580	314	11	130	700	479	1044	1.04e-135	426
Msa1006620	AT4G08850.1	33.688	282	174	6	57	331	74	349	9.99e-24	107
Msa1006620	AT4G08850.1	33.190	232	146	4	106	330	239	468	2.05e-23	107
Msa1006620	AT4G08850.1	34.389	221	138	3	117	331	202	421	1.16e-21	101
Msa1006620	AT4G08850.1	30.435	253	147	4	107	330	360	612	3.69e-18	90.1
Msa1006620	AT4G08850.1	27.597	308	141	8	102	328	256	562	3.72e-17	86.7
Msa1006620	AT1G35710.1	38.182	660	341	15	88	700	480	1119	5.82e-124	397
Msa1006620	AT1G35710.1	35.986	289	171	7	49	330	51	332	2.32e-31	132
Msa1006620	AT1G35710.1	35.294	238	144	4	102	330	240	476	1.43e-24	110
Msa1006620	AT1G35710.1	33.858	254	136	3	101	325	194	444	1.63e-24	110

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Find 142 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTTCACAATGAAGAT+GGG	0.579340	7_1:+5398721	Msa1006620:three_prime_UTR
AAAAGGCTAAATATTGTTAA+AGG	0.260643	7_1:+5397578	Msa1006620:CDS
AAAATGCTATATTGAATAGT+GGG	0.524301	7_1:+5396106	Msa1006620:CDS
AAAATTGTGGGAAATGTCAA+GGG	0.591939	7_1:-5396736	None:intergenic
AAAGGCTAAATATTGTTAAA+GGG	0.341181	7_1:+5397579	Msa1006620:CDS
AAATGTGATGTATATAGCTT+TGG	0.479124	7_1:+5398361	Msa1006620:CDS
AACTAAAACAATCATGTTAT+TGG	0.192505	7_1:-5398503	None:intergenic
AAGGCAACCACTTGGCCACA+TGG	0.585951	7_1:-5397335	None:intergenic
AAGGCTAAATATTGTTAAAG+GGG	0.513874	7_1:+5397580	Msa1006620:CDS
AAGTTAAATAACAACTCCAT+CGG	0.605502	7_1:+5396669	Msa1006620:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTCTATGGTCCAAAAATG+TGG	+	chr7_1:5396007-5396026	Msa1006620:CDS	30.0%
ATATCTCTAATCGTTGCAAT+TGG	+	chr7_1:5396157-5396176	Msa1006620:CDS	30.0%
AGCTTCATTGCAAGTTATAT+AGG	-	chr7_1:5396184-5396203	None:intergenic	30.0%
TTATCCTATAATTCCCTTGA+AGG	+	chr7_1:5396384-5396403	Msa1006620:CDS	30.0%
GATGTCAAGGTAAACTAATT+GGG	-	chr7_1:5396583-5396602	None:intergenic	30.0%
AGATGTCAAGGTAAACTAAT+TGG	-	chr7_1:5396584-5396603	None:intergenic	30.0%
TTGATCGAAGTTAGAAAACA+GGG	-	chr7_1:5396628-5396647	None:intergenic	30.0%
TAACTTCAATACTTCTAACC+TGG	-	chr7_1:5396655-5396674	None:intergenic	30.0%
AAAATTGTGGGAAATGTCAA+GGG	-	chr7_1:5396739-5396758	None:intergenic	30.0%
TTAAGCAGTAATAATCTCAC+TGG	+	chr7_1:5396882-5396901	Msa1006620:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	5395979	5398893	5395979	ID=Msa1006620;Name=Msa1006620
chr7_1	mRNA	5395979	5398893	5395979	ID=Msa1006620-mRNA-1;Parent=Msa1006620;Name=Msa1006620-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0\|1\|0.5\|1\|1\|1\|2\|176\|733
chr7_1	exon	5395979	5397794	5395979	ID=Msa1006620-mRNA-1:exon:1756;Parent=Msa1006620-mRNA-1
chr7_1	exon	5398332	5398893	5398332	ID=Msa1006620-mRNA-1:exon:1757;Parent=Msa1006620-mRNA-1
chr7_1	CDS	5395979	5397794	5395979	ID=Msa1006620-mRNA-1:cds;Parent=Msa1006620-mRNA-1
chr7_1	CDS	5398332	5398717	5398332	ID=Msa1006620-mRNA-1:cds;Parent=Msa1006620-mRNA-1
chr7_1	three_prime_UTR	5398718	5398893	5398718	ID=Msa1006620-mRNA-1:three_prime_utr;Parent=Msa1006620-mRNA-1

Gene Sequence

>Msa1006620

ATGAACTTTACAAAATATTTAGTTGCCAATTTCTATGGTCCAAAAATGTGGATGGTCTTTCTTCTTATTTGGCGTTTCATTGTGGGGACTCAATCTTCCATGGTGACATCTCAACTTCAAATGGAAGAAAATGCTATATTGAATAGTGGGTGGTGGAACGTGTCTGATGTAAACTTCAATATCTCTAATCGTTGCAATTGGACCTATATAACTTGCAATGAAGCTGGAAGCATAAAGGAAATCCTCATATACCATCAGTATGAAGAAGTGGCGTGGGGGAATGAATTGGCAACACTCAACTTGTCTACTTTTCACAATTTAGAAAGCCTTGTTATCAGGTCAGTTGAACTAGAAGGGACTATCCCAAGAGAAATTGGTCTTCTCTCCAAACTCACTCATCTCGATTTATCCTATAATTCCCTTGAAGGTGAGATACAGTATTCAATAGGAAATCTAAAACAATTAGATTACCTAGACATCTCTTATAATAAAATTCAAGGATCCATTTCTCGTGAGTTGGGGTTCCTGAAAAAACTCACAAGATTAGATCTATCTCACAATAGACTTAACAGAAGCATGCCTATTCTCCTTACAAATCTCACCCAATTAGTTTACCTTGACATCTCAAACAATTTTCTTTCTGGTTCCCTGTTTTCTAACTTCGATCAACTAACCAGGTTAGAAGTATTGAAGTTAAATAACAACTCCATCGGTGGGACCTTTCCAATTTCATTAACAAATCTCTCTCAATTACAAACCCTTGACATTTCCCACAATTTTCTCCTTGGCACCCTTCCTTCCAAAATGTTTCCGTTGAGGTCTTATGAAACTTCCATTGACCTCAGTCACAACTTCATTAGTGGTGAAATCCCTTCTGATCTTGGATATTTTTATAAACTGAATTTAAGCAGTAATAATCTCACTGGAATGGTACCCCAATCTCCCTGTAATTTCAATTTTTTGGACATTTCCTACAATTGTTTTATGGGTCCCAAATTCATTAGTTGTCTTTCGTATAGGATAACACCGTCCAAGGAGGGATATTGTCTTGGGTCCCAAATTTCATACAAGAAAAGTAATAATAATAATAAAGCAAAGAAAGATGTCGTCATTTTTCTTGCTATCATTATGATTTTCATCTTACTCTTCTCACTTATCATGTGCTTTAAACTTTATCGTGATTCTATCAAGAACAATCAAGCAAACAAAATAACAAAAAAGAATGGGGATTTTTTTTGTATATGGAATTATGATGGGAAAATAGCATATGATGACATCATTAGAGCAACTGAAGATTTTGATAATAGATATTGCATTGGGACAAGATCATATGGAAGTGTTTACAAAGCACGATTACCATGTGGCCAAGTGGTTGCCTTAAAGAAACTTCACGGTTATGAAGCAGAGGTGCAATCTTTTGATAAGAGTTTCAGAAATGAAGTGAGAATATTATCAGAAATAAAGCACCGAAATATTGTGAAGCTTTATGGATTCTGCTTGCACAAAAGAAACATGTTTTTGATATACCAGTACATGGAGAAAGGTAGCTTGTTCTCTGTCTTGTATGATGAAGTTGAAGCCGTTGAATTCAATTGGAGAAAAAGGCTAAATATTGTTAAAGGGGTTGCATTTGGTCTATCATATCTTCATCATGATTGCGCTGCTCCAATAGTGCACAGAGATGTATCTACTAGCAATATCTTGCTAAACACTGAGTGGGAGCCTACAGTTTCTGACTTTGGAACAGCTCGACTTCTTCAATATGATTCATCCAATCGAACTATAGTTGCCGGAACAATTGGTTATATAGCCCCAGAGCTTGCTTATACAATGGTTGTGAGTGAAAAATGTGATGTATATAGCTTTGGAGTAGTGGCACTTGAAAATTTAATGGGAAGACACCCTGGTGATATATTGTCATCACTTCAATCGGCATCAACTCAGGGCATGACATTATGTCAAGTATTAGACCAACGTCTCCCATTTCCCAATAACATGATTGTTTTAGTTGACATAATTCGTGTTGCCGTAGTCGCATTTGCTTGCTTAAATCTCAACCCTCGCTCTCGACCAGAAATGAAGTGTGTGTCGAAGAGTTTTGTCACAGATCTTACACCATTAAGCATTCCTTTGAGTGGAATTTCAGTGCAGCAACTCATGAGTCAAGAACTGAAAACATCGTTTCATATCGGGAACCGTTGA

Protein sequence

>Msa1006620

MNFTKYLVANFYGPKMWMVFLLIWRFIVGTQSSMVTSQLQMEENAILNSGWWNVSDVNFNISNRCNWTYITCNEAGSIKEILIYHQYEEVAWGNELATLNLSTFHNLESLVIRSVELEGTIPREIGLLSKLTHLDLSYNSLEGEIQYSIGNLKQLDYLDISYNKIQGSISRELGFLKKLTRLDLSHNRLNRSMPILLTNLTQLVYLDISNNFLSGSLFSNFDQLTRLEVLKLNNNSIGGTFPISLTNLSQLQTLDISHNFLLGTLPSKMFPLRSYETSIDLSHNFISGEIPSDLGYFYKLNLSSNNLTGMVPQSPCNFNFLDISYNCFMGPKFISCLSYRITPSKEGYCLGSQISYKKSNNNNKAKKDVVIFLAIIMIFILLFSLIMCFKLYRDSIKNNQANKITKKNGDFFCIWNYDGKIAYDDIIRATEDFDNRYCIGTRSYGSVYKARLPCGQVVALKKLHGYEAEVQSFDKSFRNEVRILSEIKHRNIVKLYGFCLHKRNMFLIYQYMEKGSLFSVLYDEVEAVEFNWRKRLNIVKGVAFGLSYLHHDCAAPIVHRDVSTSNILLNTEWEPTVSDFGTARLLQYDSSNRTIVAGTIGYIAPELAYTMVVSEKCDVYSFGVVALENLMGRHPGDILSSLQSASTQGMTLCQVLDQRLPFPNNMIVLVDIIRVAVVAFACLNLNPRSRPEMKCVSKSFVTDLTPLSIPLSGISVQQLMSQELKTSFHIGNR*