Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021460 | Msa0629940 | 0.836848 | 7.355897e-57 | -8.615850e-47 |
| Msa0109970 | Msa0629940 | 0.818094 | 2.345365e-52 | -8.615850e-47 |
| Msa0109990 | Msa0629940 | 0.823859 | 1.104062e-53 | -8.615850e-47 |
| Msa0629850 | Msa0629940 | 0.803476 | 3.422535e-49 | -8.615850e-47 |
| Msa0629930 | Msa0629940 | 0.856659 | 2.769544e-62 | -8.615850e-47 |
| Msa0629940 | Msa0641900 | 0.817695 | 2.886184e-52 | -8.615850e-47 |
| Msa0629940 | Msa0668150 | 0.803903 | 2.790861e-49 | -8.615850e-47 |
| Msa0629940 | Msa0696800 | 0.805413 | 1.351328e-49 | -8.615850e-47 |
| Msa0629940 | Msa0818010 | 0.849547 | 3.015784e-60 | -8.615850e-47 |
| Msa0629940 | Msa0818030 | 0.841473 | 4.651647e-58 | -8.615850e-47 |
| Msa0629940 | Msa0858360 | 0.831477 | 1.632698e-55 | -8.615850e-47 |
| Msa0629940 | Msa0884920 | 0.807251 | 5.537575e-50 | -8.615850e-47 |
| Msa0629940 | Msa0902020 | 0.804255 | 2.358498e-49 | -8.615850e-47 |
| Msa0629940 | Msa0912500 | 0.819437 | 1.161760e-52 | -8.615850e-47 |
| Msa0629940 | Msa1006250 | 0.873790 | 1.119164e-67 | -8.615850e-47 |
| Msa0629940 | Msa1006400 | 0.823119 | 1.644008e-53 | -8.615850e-47 |
| Msa0629940 | Msa1006440 | 0.802386 | 5.749030e-49 | -8.615850e-47 |
| Msa0629940 | Msa1006620 | 0.804187 | 2.436860e-49 | -8.615850e-47 |
| Msa0629940 | Msa1009270 | 0.843328 | 1.499616e-58 | -8.615850e-47 |
| Msa0629940 | Msa1027240 | 0.817229 | 3.674758e-52 | -8.615850e-47 |
| Msa0629940 | Msa1048490 | 0.829799 | 4.205846e-55 | -8.615850e-47 |
| Msa0629940 | Msa1048610 | 0.817125 | 3.877626e-52 | -8.615850e-47 |
| Msa0629940 | Msa1050480 | 0.828265 | 9.897790e-55 | -8.615850e-47 |
| Msa0629940 | Msa1050490 | 0.802182 | 6.333211e-49 | -8.615850e-47 |
| Msa0629940 | Msa1050500 | 0.814487 | 1.502550e-51 | -8.615850e-47 |
| Msa0629940 | Msa1100180 | 0.842668 | 2.247303e-58 | -8.615850e-47 |
| Msa0629940 | Msa1139240 | 0.802440 | 5.605173e-49 | -8.615850e-47 |
| Msa0629940 | Msa1198090 | 0.825513 | 4.499394e-54 | -8.615850e-47 |
| Msa0629940 | Msa1279860 | 0.808712 | 2.707327e-50 | -8.615850e-47 |
| Msa0629940 | Msa1287700 | 0.803464 | 3.443133e-49 | -8.615850e-47 |
| Msa0629940 | Msa1288000 | 0.821485 | 3.938048e-53 | -8.615850e-47 |
| Msa0629940 | Msa1289540 | 0.826655 | 2.408241e-54 | -8.615850e-47 |
| Msa0629940 | Msa1351080 | 0.810724 | 9.996187e-51 | -8.615850e-47 |
| Msa0629940 | Msa1359820 | 0.812401 | 4.318079e-51 | -8.615850e-47 |
| Msa0629940 | Msa1406600 | 0.829001 | 6.571111e-55 | -8.615850e-47 |
| Msa0629940 | Msa1444370 | 0.809709 | 1.654482e-50 | -8.615850e-47 |
| Msa0142230 | Msa0629940 | 0.840457 | 8.595475e-58 | -8.615850e-47 |
| Msa0156170 | Msa0629940 | 0.808273 | 3.358807e-50 | -8.615850e-47 |
| Msa0203070 | Msa0629940 | 0.824672 | 7.110099e-54 | -8.615850e-47 |
| Msa0211670 | Msa0629940 | 0.821085 | 4.870149e-53 | -8.615850e-47 |
| Msa0211680 | Msa0629940 | 0.806834 | 6.785893e-50 | -8.615850e-47 |
| Msa0235190 | Msa0629940 | 0.800768 | 1.234623e-48 | -8.615850e-47 |
| Msa0389030 | Msa0629940 | 0.810084 | 1.373866e-50 | -8.615850e-47 |
| Msa0452410 | Msa0629940 | 0.817579 | 3.065179e-52 | -8.615850e-47 |
| Msa0458700 | Msa0629940 | 0.801071 | 1.070505e-48 | -8.615850e-47 |
| Msa0273570 | Msa0629940 | 0.825404 | 4.775879e-54 | -8.615850e-47 |
| Msa0284810 | Msa0629940 | 0.835144 | 1.991402e-56 | -8.615850e-47 |
| Msa0323550 | Msa0629940 | 0.853368 | 2.502943e-61 | -8.615850e-47 |
| Msa0352010 | Msa0629940 | 0.802819 | 4.681933e-49 | -8.615850e-47 |
| Msa0533430 | Msa0629940 | 0.834951 | 2.227470e-56 | -8.615850e-47 |
| Msa0580610 | Msa0629940 | 0.803405 | 3.541376e-49 | -8.615850e-47 |
| Msa0584130 | Msa0629940 | 0.806287 | 8.852165e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGAGGTCTTAGACCTTTC+AGG | 0.195336 | 4_3:+11333699 | Msa0629940:five_prime_UTR |
| AATTGTTCTCTCTCCACTTT+AGG | 0.232152 | 4_3:+11333804 | Msa0629940:three_prime_UTR |
| TAGTTCTAGCCGACTAAATT+TGG | 0.258819 | 4_3:-11333914 | None:intergenic |
| GTCTTAGACCTTTCAGGAAA+TGG | 0.299834 | 4_3:+11333705 | Msa0629940:five_prime_UTR |
| TGCATGGATTCACATCATTA+AGG | 0.305907 | 4_3:+11333754 | Msa0629940:exon |
| ATTTAGTCGGCTAGAACTAT+TGG | 0.317235 | 4_3:+11333918 | Msa0629940:three_prime_UTR |
| GAGAGAACAATTGAACTTGT+TGG | 0.397525 | 4_3:-11333793 | None:intergenic |
| TCTTAGACCTTTCAGGAAAT+GGG | 0.437040 | 4_3:+11333706 | Msa0629940:five_prime_UTR |
| TTGAAGATGTGTACCTAAAG+TGG | 0.465229 | 4_3:-11333817 | None:intergenic |
| TCGGCTAGAACTATTGGACT+TGG | 0.481181 | 4_3:+11333924 | Msa0629940:three_prime_UTR |
| TTCGAGATCAAAGGAGTTGG+AGG | 0.491908 | 4_3:+11333683 | Msa0629940:five_prime_UTR |
| AGTTTAGCCCATTTCCTGAA+AGG | 0.528093 | 4_3:-11333713 | None:intergenic |
| AACATCATATCAAGTTTGCA+TGG | 0.579307 | 4_3:+11333738 | Msa0629940:exon |
| CGGCTAGAACTATTGGACTT+GGG | 0.601856 | 4_3:+11333925 | Msa0629940:three_prime_UTR |
| ACTAGATCTCTACATGCTGA+AGG | 0.603184 | 4_3:+11333961 | Msa0629940:three_prime_UTR |
| CTAGAACTATTGGACTTGGG+TGG | 0.661185 | 4_3:+11333928 | Msa0629940:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACATCATATCAAGTTTGCA+TGG | + | chr4_3:11333738-11333757 | Msa0629940:exon | 30.0% | |
| ! | CAGATTTTGCCAAATTTAGT+CGG | + | chr4_3:11333905-11333924 | Msa0629940:intron | 30.0% |
| !! | TCAAGGATTTTCGAGATCAA+AGG | + | chr4_3:11333674-11333693 | Msa0629940:five_prime_UTR | 35.0% |
| !! | ATTTTCGAGATCAAAGGAGT+TGG | + | chr4_3:11333680-11333699 | Msa0629940:five_prime_UTR | 35.0% |
| TCTTAGACCTTTCAGGAAAT+GGG | + | chr4_3:11333706-11333725 | Msa0629940:five_prime_UTR | 35.0% | |
| TGCATGGATTCACATCATTA+AGG | + | chr4_3:11333754-11333773 | Msa0629940:exon | 35.0% | |
| GAGAGAACAATTGAACTTGT+TGG | - | chr4_3:11333796-11333815 | None:intergenic | 35.0% | |
| AATTGTTCTCTCTCCACTTT+AGG | + | chr4_3:11333804-11333823 | Msa0629940:three_prime_UTR | 35.0% | |
| TTGAAGATGTGTACCTAAAG+TGG | - | chr4_3:11333820-11333839 | None:intergenic | 35.0% | |
| ATTTGGCAAAATCTGTTGCA+AGG | - | chr4_3:11333900-11333919 | None:intergenic | 35.0% | |
| !! | TAGTTCTAGCCGACTAAATT+TGG | - | chr4_3:11333917-11333936 | None:intergenic | 35.0% |
| ATTTAGTCGGCTAGAACTAT+TGG | + | chr4_3:11333918-11333937 | Msa0629940:three_prime_UTR | 35.0% | |
| GTCTTAGACCTTTCAGGAAA+TGG | + | chr4_3:11333705-11333724 | Msa0629940:five_prime_UTR | 40.0% | |
| AGTTTAGCCCATTTCCTGAA+AGG | - | chr4_3:11333716-11333735 | None:intergenic | 40.0% | |
| ACTAGATCTCTACATGCTGA+AGG | + | chr4_3:11333961-11333980 | Msa0629940:three_prime_UTR | 40.0% | |
| TTCGAGATCAAAGGAGTTGG+AGG | + | chr4_3:11333683-11333702 | Msa0629940:five_prime_UTR | 45.0% | |
| TTGGAGGTCTTAGACCTTTC+AGG | + | chr4_3:11333699-11333718 | Msa0629940:five_prime_UTR | 45.0% | |
| TCGGCTAGAACTATTGGACT+TGG | + | chr4_3:11333924-11333943 | Msa0629940:three_prime_UTR | 45.0% | |
| CGGCTAGAACTATTGGACTT+GGG | + | chr4_3:11333925-11333944 | Msa0629940:three_prime_UTR | 45.0% | |
| CTAGAACTATTGGACTTGGG+TGG | + | chr4_3:11333928-11333947 | Msa0629940:three_prime_UTR | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 11333674 | 11334010 | 11333674 | ID=Msa0629940;Name=Msa0629940 |
| chr4_3 | mRNA | 11333674 | 11334010 | 11333674 | ID=Msa0629940-mRNA-1;Parent=Msa0629940;Name=Msa0629940-mRNA-1;_AED=0.00;_eAED=0.00;_QI=83|1|1|1|1|1|2|154|5 |
| chr4_3 | exon | 11333674 | 11333825 | 11333674 | ID=Msa0629940-mRNA-1:exon:3400;Parent=Msa0629940-mRNA-1 |
| chr4_3 | exon | 11333908 | 11334010 | 11333908 | ID=Msa0629940-mRNA-1:exon:3401;Parent=Msa0629940-mRNA-1 |
| chr4_3 | five_prime_UTR | 11333674 | 11333756 | 11333674 | ID=Msa0629940-mRNA-1:five_prime_utr;Parent=Msa0629940-mRNA-1 |
| chr4_3 | CDS | 11333757 | 11333774 | 11333757 | ID=Msa0629940-mRNA-1:cds;Parent=Msa0629940-mRNA-1 |
| chr4_3 | three_prime_UTR | 11333775 | 11333825 | 11333775 | ID=Msa0629940-mRNA-1:three_prime_utr;Parent=Msa0629940-mRNA-1 |
| chr4_3 | three_prime_UTR | 11333908 | 11334010 | 11333908 | ID=Msa0629940-mRNA-1:three_prime_utr;Parent=Msa0629940-mRNA-1 |
| Gene Sequence |
| Protein sequence |
