AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1013950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1013950 MtrunA17_Chr2g0277111 81.183 186 27 1 22 199 157 342 1.87e-104 310
Msa1013950 MtrunA17_Chr2g0309651 35.533 197 106 7 11 199 251 434 4.12e-27 108
Msa1013950 MtrunA17_Chr4g0069011 50.000 78 39 0 122 199 315 392 2.13e-20 89.0
Msa1013950 MtrunA17_Chr2g0293641 38.462 117 65 2 90 199 310 426 8.68e-20 87.4
Msa1013950 MtrunA17_Chr8g0380431 49.412 85 39 1 115 199 397 477 2.57e-18 82.8
Msa1013950 MtrunA17_Chr7g0253191 33.908 174 75 7 41 197 331 481 4.55e-18 82.4
Msa1013950 MtrunA17_Chr6g0461531 50.000 74 33 1 124 197 400 469 6.72e-17 79.0
Msa1013950 MtrunA17_Chr4g0000071 51.429 70 30 1 128 197 384 449 8.61e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1013950 AT1G09810.2 51.282 117 54 2 86 199 133 249 6.54e-29 112
Msa1013950 AT1G09810.3 51.282 117 54 2 86 199 156 272 7.31e-29 112
Msa1013950 AT1G09810.1 51.282 117 54 2 86 199 175 291 7.67e-29 112
Msa1013950 AT1G79270.2 53.165 79 37 0 121 199 288 366 9.96e-24 99.0
Msa1013950 AT1G79270.4 53.165 79 37 0 121 199 288 366 9.96e-24 99.0
Msa1013950 AT1G79270.3 53.165 79 37 0 121 199 288 366 9.96e-24 99.0
Msa1013950 AT1G79270.1 53.165 79 37 0 121 199 303 381 1.17e-23 98.6
Msa1013950 AT5G58190.1 43.636 110 50 3 98 199 275 380 9.26e-21 90.5
Msa1013950 AT5G58190.2 43.636 110 50 3 98 199 276 381 9.73e-21 90.1
Msa1013950 AT1G55500.2 43.220 118 50 4 95 199 357 470 7.08e-20 87.8
Msa1013950 AT1G55500.3 43.220 118 50 4 95 199 344 457 7.32e-20 87.8
Msa1013950 AT3G17330.2 50.633 79 38 1 125 202 154 232 7.42e-20 87.8
Msa1013950 AT1G55500.5 43.220 118 50 4 95 199 357 470 7.75e-20 87.8
Msa1013950 AT1G55500.4 43.220 118 50 4 95 199 357 470 8.22e-20 87.8
Msa1013950 AT1G55500.1 43.220 118 50 4 95 199 301 414 9.03e-20 87.4
Msa1013950 AT1G55500.6 43.220 118 50 4 95 199 314 427 9.24e-20 87.4
Msa1013950 AT3G17330.1 50.633 79 38 1 125 202 256 334 1.23e-19 87.0
Msa1013950 AT3G17330.3 50.633 79 38 1 125 202 256 334 1.23e-19 87.0
Msa1013950 AT3G13460.3 40.984 122 50 3 95 198 380 497 2.25e-19 86.3
Msa1013950 AT1G48110.2 47.561 82 42 1 126 206 308 389 2.51e-19 86.3
Msa1013950 AT1G48110.1 47.561 82 42 1 126 206 308 389 2.51e-19 86.3
Msa1013950 AT3G13460.1 39.837 123 52 3 95 199 380 498 6.23e-19 85.1
Msa1013950 AT3G13460.2 39.837 123 52 3 95 199 377 495 6.28e-19 85.1
Msa1013950 AT3G13460.4 39.837 123 52 3 95 199 379 497 6.35e-19 85.1
Msa1013950 AT5G61020.1 39.231 130 59 3 85 199 193 317 1.96e-18 83.6
Msa1013950 AT5G61020.2 39.231 130 59 3 85 199 191 315 2.11e-18 83.6
Msa1013950 AT3G03950.3 37.500 120 65 4 85 199 187 301 2.56e-15 74.3
Msa1013950 AT3G03950.2 37.500 120 65 4 85 199 183 297 2.58e-15 74.3
Msa1013950 AT3G03950.1 37.500 120 65 4 85 199 184 298 2.90e-15 74.3
Msa1013950 AT3G13060.5 35.897 117 57 3 91 197 344 452 7.45e-15 73.2
Msa1013950 AT3G13060.2 35.294 119 59 3 91 199 344 454 8.68e-15 73.2
Msa1013950 AT3G13060.4 35.294 119 59 3 91 199 334 444 8.80e-15 73.2
Msa1013950 AT3G13060.3 35.294 119 59 3 91 199 322 432 9.53e-15 72.8
Msa1013950 AT3G13060.6 35.294 119 59 3 91 199 322 432 9.53e-15 72.8
Msa1013950 AT3G13060.1 35.294 119 59 3 91 199 344 454 9.73e-15 72.8
Msa1013950 AT1G27960.2 36.607 112 60 3 91 202 289 389 1.44e-14 72.4
Msa1013950 AT1G27960.3 38.095 105 54 3 95 199 219 312 1.90e-14 72.0
Msa1013950 AT1G27960.1 38.095 105 54 3 95 199 293 386 2.20e-14 72.0

Find 87 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTATATGCCACTTAAT+TGG 0.122163 7_1:+21461042 None:intergenic
AGATCAAACCAAGAGATATT+TGG 0.251737 7_1:-21460129 Msa1013950:three_prime_UTR
TTGATTTAGCTTCTGCGTTT+TGG 0.256390 7_1:+21461461 None:intergenic
GATGAACATTCTTTATAATA+TGG 0.271698 7_1:-21461259 Msa1013950:three_prime_UTR
TACTTGAGTCAATCAGATTA+AGG 0.280024 7_1:-21460237 Msa1013950:three_prime_UTR
TAGCAGTTTCATATTTAGTT+TGG 0.294839 7_1:+21461590 None:intergenic
CCCAATCTTCCTCTTCTTCT+CGG 0.317116 7_1:-21461419 Msa1013950:CDS
TCATATTTAGTTTGGAAATC+TGG 0.320309 7_1:+21461598 None:intergenic
TCTTTCACAATACTAATTAC+AGG 0.330280 7_1:-21461825 Msa1013950:CDS
TCCTTAACAACTGATGATTC+CGG 0.338646 7_1:+21461661 None:intergenic
TATAATATGGTGTCAATGAT+TGG 0.348568 7_1:-21461246 Msa1013950:three_prime_UTR
ACCTGTTCTCATTGTTTGTA+AGG 0.359165 7_1:+21460986 None:intergenic
TATTTGTTGTCAAATTAATC+AGG 0.371737 7_1:-21461169 Msa1013950:three_prime_UTR
TTATTATATGCCACTTAATT+GGG 0.372548 7_1:+21461043 None:intergenic
AGGGAATAAATAATGATGTT+TGG 0.381941 7_1:-21461525 Msa1013950:CDS
TTGTGAAAGACATGGCTTCT+TGG 0.385400 7_1:+21461838 None:intergenic
TCTCTGCTACTCCAACAAAT+TGG 0.385829 7_1:+21461133 None:intergenic
TATGCTGCTCGGTAATCCTC+TGG 0.390833 7_1:+21460716 None:intergenic
TCATTAGATACTATACCATT+TGG 0.393991 7_1:+21461778 None:intergenic
CCTTAACAACTGATGATTCC+GGG 0.395222 7_1:+21461662 None:intergenic
ATGTCGAAGCATTTCGTGTT+TGG 0.395427 7_1:+21461869 None:intergenic
TGTGAAAGACATGGCTTCTT+GGG 0.396327 7_1:+21461839 None:intergenic
CCCGGAATCATCAGTTGTTA+AGG 0.398116 7_1:-21461662 Msa1013950:CDS
ACCGAGAAGAAGAGGAAGAT+TGG 0.402582 7_1:+21461418 None:intergenic
ACTTAAGATATTGATATTGT+TGG 0.413309 7_1:-21461331 Msa1013950:exon
AATGCAAGTGGCCAATTTGT+TGG 0.419888 7_1:-21461144 Msa1013950:three_prime_UTR
CAATGAAGATCACATTCAAA+AGG 0.420815 7_1:-21461545 Msa1013950:CDS
TCAGGCAAAGACATCTCTAT+TGG 0.426726 7_1:-21460782 Msa1013950:three_prime_UTR
ATTGTAAACCAAATATCTCT+TGG 0.430496 7_1:+21460121 None:intergenic
GATTGGACTAAAAGATGGTC+TGG 0.434011 7_1:-21460830 Msa1013950:three_prime_UTR
CTAGTTCAGTCATGGTTGAA+TGG 0.439966 7_1:+21460084 None:intergenic
GTTGTAAGTTCTTCGAGGGT+CGG 0.440776 7_1:+21461891 None:intergenic
TCAGTACTATCAAAACTTGT+CGG 0.441080 7_1:-21460152 Msa1013950:three_prime_UTR
TTTGATCAGCGTCTCATGAT+TGG 0.450002 7_1:+21460431 None:intergenic
CCGAGAAGAAGAGGAAGATT+GGG 0.454266 7_1:+21461419 None:intergenic
TAAGATATTGATATTGTTGG+AGG 0.457098 7_1:-21461328 Msa1013950:three_prime_UTR
CTGTAGTTAGTAGAGGCACT+TGG 0.483395 7_1:+21461802 None:intergenic
AATCTCTCTCTCACTCCCTC+TGG 0.485346 7_1:-21460399 Msa1013950:three_prime_UTR
TCCTTACAAACAATGAGAAC+AGG 0.488032 7_1:-21460987 Msa1013950:three_prime_UTR
AAGAAATGACTCGGGGTCCT+CGG 0.496517 7_1:-21461705 Msa1013950:CDS
TTTGTCATCTAGTTCAGTCA+TGG 0.498391 7_1:+21460076 None:intergenic
CTCACTCCCTCTGGAAGACA+TGG 0.499578 7_1:-21460390 Msa1013950:three_prime_UTR
CGGCTGCTGTAAAATTAGAA+TGG 0.501961 7_1:+21461911 None:intergenic
AATGAAGATCACATTCAAAA+GGG 0.506544 7_1:-21461544 Msa1013950:CDS
TCTACTAACTACAGACCAAA+TGG 0.507075 7_1:-21461793 Msa1013950:CDS
ACGGATTCTTCCCAATTAAG+TGG 0.508762 7_1:-21461053 Msa1013950:three_prime_UTR
TTGCAGATTGGACTAAAAGA+TGG 0.509143 7_1:-21460835 Msa1013950:intron
GTTGGAGTAGCAGAGATGCT+AGG 0.509883 7_1:-21461126 Msa1013950:three_prime_UTR
TTTGGTTGTAAGTTCTTCGA+GGG 0.514819 7_1:+21461887 None:intergenic
GTTTGGTTGTAAGTTCTTCG+AGG 0.516731 7_1:+21461886 None:intergenic
TTGAACTCACCGAGAAGAAG+AGG 0.527322 7_1:+21461410 None:intergenic
TTGGAGTAGCAGAGATGCTA+GGG 0.531951 7_1:-21461125 Msa1013950:three_prime_UTR
GAAGCTAAATCAAGTGAGAC+AGG 0.536998 7_1:-21461451 Msa1013950:CDS
GATCAGCGTCTCATGATTGG+TGG 0.541226 7_1:+21460434 None:intergenic
ATGTGTCGAAACAAACAGTT+GGG 0.547018 7_1:+21461012 None:intergenic
AGGATTACCGAGCAGCATAC+AGG 0.552460 7_1:-21460712 Msa1013950:three_prime_UTR
AGCAGAGATGCTAGGGCCGG+TGG 0.553830 7_1:-21461118 Msa1013950:three_prime_UTR
AAAGACATGGCTTCTTGGGT+TGG 0.559310 7_1:+21461843 None:intergenic
AAGTGTCAAAAGAAATGACT+CGG 0.565132 7_1:-21461714 Msa1013950:CDS
ACAGGTGTGTAACTTTCAGT+AGG 0.575402 7_1:-21460969 Msa1013950:three_prime_UTR
ATGCTGCTCGGTAATCCTCT+GGG 0.580878 7_1:+21460717 None:intergenic
GATGTGTCGAAACAAACAGT+TGG 0.582692 7_1:+21461011 None:intergenic
AGTACTGAGAAATATATACA+TGG 0.583571 7_1:+21460167 None:intergenic
ATTTAGTTTGGAAATCTGGA+AGG 0.584834 7_1:+21461602 None:intergenic
TCTAAAGCCATGTCTTCCAG+AGG 0.588899 7_1:+21460383 None:intergenic
ATTTGGTCTGTAGTTAGTAG+AGG 0.589027 7_1:+21461795 None:intergenic
TGGAAACTTGACAAATACAA+CGG 0.589722 7_1:-21461072 Msa1013950:three_prime_UTR
AAAACAGATATGCAATCAAG+TGG 0.597901 7_1:-21460459 Msa1013950:three_prime_UTR
GCAGAAGAACAAGGCAGATG+TGG 0.598529 7_1:-21462182 Msa1013950:intron
AACAACTGATGATTCCGGGT+TGG 0.603971 7_1:+21461666 None:intergenic
TGTCGTGCCTGTATGCTGCT+CGG 0.604290 7_1:+21460705 None:intergenic
AGTGTCAAAAGAAATGACTC+GGG 0.607102 7_1:-21461713 Msa1013950:CDS
ATTAATCAGGTAAATGCAAG+TGG 0.607713 7_1:-21461156 Msa1013950:three_prime_UTR
TCATAGTCAGCAGAAGAACA+AGG 0.611480 7_1:-21462191 Msa1013950:CDS
AATTAGTATTGTGAAAGACA+TGG 0.624561 7_1:+21461830 None:intergenic
TTCAACTTCTTATTTCCATG+TGG 0.626711 7_1:+21461493 None:intergenic
AGTAGCAGAGATGCTAGGGC+CGG 0.626729 7_1:-21461121 Msa1013950:three_prime_UTR
CAGGTGTGTAACTTTCAGTA+GGG 0.627821 7_1:-21460968 Msa1013950:three_prime_UTR
GTTTGGACAAGCACTCCACA+TGG 0.628206 7_1:-21461508 Msa1013950:CDS
CTAAAGCCATGTCTTCCAGA+GGG 0.641953 7_1:+21460384 None:intergenic
TTTCAGTAGGGACACACAAG+AGG 0.654133 7_1:-21460956 Msa1013950:intron
AGAAGTTATTTCGTTCCCAG+AGG 0.656111 7_1:-21460732 Msa1013950:three_prime_UTR
AGACGCTGATCAAACTAACA+AGG 0.658325 7_1:-21460422 Msa1013950:three_prime_UTR
TCATCAAAACAATTCCAACC+CGG 0.676336 7_1:-21461680 Msa1013950:CDS
GTACTGAGAAATATATACAT+GGG 0.677984 7_1:+21460168 None:intergenic
GTGTCAAAAGAAATGACTCG+GGG 0.701528 7_1:-21461712 Msa1013950:CDS
GTAAAATTAGAATGGAAACG+AGG 0.766321 7_1:+21461919 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTTATGTTTTTCATA+TGG - chr7_1:21460369-21460388 Msa1013950:three_prime_UTR 10.0%
!! AAAAATATTCAAAAGTTATC+AGG - chr7_1:21461841-21461860 Msa1013950:CDS 15.0%
!!! TTAATTGGTTTCTCTTTTAT+TGG - chr7_1:21460344-21460363 Msa1013950:three_prime_UTR 20.0%
!! ACTTAAGATATTGATATTGT+TGG - chr7_1:21461310-21461329 Msa1013950:three_prime_UTR 20.0%
!! GATGAACATTCTTTATAATA+TGG - chr7_1:21461382-21461401 Msa1013950:CDS 20.0%
!!! TATTTGTTGTCAAATTAATC+AGG - chr7_1:21461472-21461491 Msa1013950:CDS 20.0%
!!! TCAAAAAAGACATGAAATTT+TGG - chr7_1:21461549-21461568 Msa1013950:CDS 20.0%
!!! TTATTATATGCCACTTAATT+GGG + chr7_1:21461601-21461620 None:intergenic 20.0%
!!! TTTATTATATGCCACTTAAT+TGG + chr7_1:21461602-21461621 None:intergenic 20.0%
!! TTATCAAACCACAAAAAATT+TGG + chr7_1:21462329-21462348 None:intergenic 20.0%
! AATCTTTCATTTGTCTGAAA+TGG - chr7_1:21460284-21460303 Msa1013950:three_prime_UTR 25.0%
! TATTTCCTAAAGAGATGAAA+GGG + chr7_1:21460575-21460594 None:intergenic 25.0%
! TTATTTCCTAAAGAGATGAA+AGG + chr7_1:21460576-21460595 None:intergenic 25.0%
! AACAACAATGACATTTCTAT+CGG + chr7_1:21460614-21460633 None:intergenic 25.0%
! GATTACAAAATGCATTGTTT+AGG - chr7_1:21460637-21460656 Msa1013950:intron 25.0%
! AATTAGTATTGTGAAAGACA+TGG + chr7_1:21460814-21460833 None:intergenic 25.0%
! TCTTTCACAATACTAATTAC+AGG - chr7_1:21460816-21460835 Msa1013950:three_prime_UTR 25.0%
! TCATTAGATACTATACCATT+TGG + chr7_1:21460866-21460885 None:intergenic 25.0%
!!! TCATATTTAGTTTGGAAATC+TGG + chr7_1:21461046-21461065 None:intergenic 25.0%
! TAGCAGTTTCATATTTAGTT+TGG + chr7_1:21461054-21461073 None:intergenic 25.0%
! AATGAAGATCACATTCAAAA+GGG - chr7_1:21461097-21461116 Msa1013950:three_prime_UTR 25.0%
! AGGGAATAAATAATGATGTT+TGG - chr7_1:21461116-21461135 Msa1013950:three_prime_UTR 25.0%
!!! GCCATTTTTTTAATACAAGA+TGG - chr7_1:21461277-21461296 Msa1013950:three_prime_UTR 25.0%
! GCCATCTTGTATTAAAAAAA+TGG + chr7_1:21461281-21461300 None:intergenic 25.0%
! TAAGATATTGATATTGTTGG+AGG - chr7_1:21461313-21461332 Msa1013950:three_prime_UTR 25.0%
!! TATAATATGGTGTCAATGAT+TGG - chr7_1:21461395-21461414 Msa1013950:CDS 25.0%
!!! ACTAGTTTTTAAATGTAGGA+AGG + chr7_1:21461434-21461453 None:intergenic 25.0%
! TTCCTACATTTAAAAACTAG+TGG - chr7_1:21461433-21461452 Msa1013950:CDS 25.0%
!!! AACCACTAGTTTTTAAATGT+AGG + chr7_1:21461438-21461457 None:intergenic 25.0%
! ACCAAGAAAATACCAAAAAA+TGG - chr7_1:21462299-21462318 Msa1013950:intron 25.0%
!!! TCCATTTTTTGGTATTTTCT+TGG + chr7_1:21462303-21462322 None:intergenic 25.0%
!!! AAATTTGGTCATCCATTTTT+TGG + chr7_1:21462314-21462333 None:intergenic 25.0%
! GTACTGAGAAATATATACAT+GGG + chr7_1:21462476-21462495 None:intergenic 25.0%
!! AGTACTGAGAAATATATACA+TGG + chr7_1:21462477-21462496 None:intergenic 25.0%
! ATTGTAAACCAAATATCTCT+TGG + chr7_1:21462523-21462542 None:intergenic 25.0%
ATTAAGAGTCCAAATAAGAC+TGG + chr7_1:21460181-21460200 None:intergenic 30.0%
TATTTGAACGCTCATTTAAG+TGG - chr7_1:21460248-21460267 Msa1013950:three_prime_UTR 30.0%
ATAGATTGAGCTATATCACA+TGG + chr7_1:21460412-21460431 None:intergenic 30.0%
! CAAAATGCATTGTTTAGGTT+TGG - chr7_1:21460642-21460661 Msa1013950:intron 30.0%
!!! AAGTGAATGTGTTATTTTGC+AGG - chr7_1:21460697-21460716 Msa1013950:three_prime_UTR 30.0%
GTAAAATTAGAATGGAAACG+AGG + chr7_1:21460725-21460744 None:intergenic 30.0%
AAGTGTCAAAAGAAATGACT+CGG - chr7_1:21460927-21460946 Msa1013950:intron 30.0%
!! ATTTAGTTTGGAAATCTGGA+AGG + chr7_1:21461042-21461061 None:intergenic 30.0%
CAATGAAGATCACATTCAAA+AGG - chr7_1:21461096-21461115 Msa1013950:three_prime_UTR 30.0%
! TTCAACTTCTTATTTCCATG+TGG + chr7_1:21461151-21461170 None:intergenic 30.0%
ATTAATCAGGTAAATGCAAG+TGG - chr7_1:21461485-21461504 Msa1013950:CDS 30.0%
TGGAAACTTGACAAATACAA+CGG - chr7_1:21461569-21461588 Msa1013950:CDS 30.0%
CAAAGGAAATAGAACTTACA+TGG + chr7_1:21461988-21462007 None:intergenic 30.0%
! TATTCAAGTGTTCTAGACAA+AGG + chr7_1:21462005-21462024 None:intergenic 30.0%
AAAACAGATATGCAATCAAG+TGG - chr7_1:21462182-21462201 Msa1013950:intron 30.0%
! TTTGGTATTTTCTTGGTCTA+TGG + chr7_1:21462296-21462315 None:intergenic 30.0%
!!! ATGGATGACCAAATTTTTTG+TGG - chr7_1:21462318-21462337 Msa1013950:intron 30.0%
TACTTGAGTCAATCAGATTA+AGG - chr7_1:21462404-21462423 Msa1013950:intron 30.0%
TCAGTACTATCAAAACTTGT+CGG - chr7_1:21462489-21462508 Msa1013950:intron 30.0%
AGATCAAACCAAGAGATATT+TGG - chr7_1:21462512-21462531 Msa1013950:intron 30.0%
CAAAAGAATTCCATCCTTTC+AGG + chr7_1:21460139-21460158 None:intergenic 35.0%
CTCATTTAAGTGGAAGAAGA+CGG - chr7_1:21460258-21460277 Msa1013950:three_prime_UTR 35.0%
TCATTTAAGTGGAAGAAGAC+GGG - chr7_1:21460259-21460278 Msa1013950:three_prime_UTR 35.0%
CCAATTAAAACCACGTTGAA+GGG + chr7_1:21460332-21460351 None:intergenic 35.0%
ACCAATTAAAACCACGTTGA+AGG + chr7_1:21460333-21460352 None:intergenic 35.0%
CTTGACTCTCTCTATGAATT+TGG - chr7_1:21460510-21460529 Msa1013950:intron 35.0%
TTAGACCCTTTCATCTCTTT+AGG - chr7_1:21460567-21460586 Msa1013950:intron 35.0%
! AATGCATTGTTTAGGTTTGG+TGG - chr7_1:21460645-21460664 Msa1013950:intron 35.0%
TTTGGTTGTAAGTTCTTCGA+GGG + chr7_1:21460757-21460776 None:intergenic 35.0%
ATTTGGTCTGTAGTTAGTAG+AGG + chr7_1:21460849-21460868 None:intergenic 35.0%
TCTACTAACTACAGACCAAA+TGG - chr7_1:21460848-21460867 Msa1013950:intron 35.0%
AGTGTCAAAAGAAATGACTC+GGG - chr7_1:21460928-21460947 Msa1013950:intron 35.0%
!!! GAATTGTTTTGATGAAACCG+AGG + chr7_1:21460956-21460975 None:intergenic 35.0%
TCATCAAAACAATTCCAACC+CGG - chr7_1:21460961-21460980 Msa1013950:three_prime_UTR 35.0%
TCCTTAACAACTGATGATTC+CGG + chr7_1:21460983-21461002 None:intergenic 35.0%
!! TTGATTTAGCTTCTGCGTTT+TGG + chr7_1:21461183-21461202 None:intergenic 35.0%
AAAAATGGCAAGCGAAAACT+TGG + chr7_1:21461266-21461285 None:intergenic 35.0%
!!! ATGTCTTTTTTGAAGTCCAC+CGG + chr7_1:21461542-21461561 None:intergenic 35.0%
ATGTGTCGAAACAAACAGTT+GGG + chr7_1:21461632-21461651 None:intergenic 35.0%
TCCTTACAAACAATGAGAAC+AGG - chr7_1:21461654-21461673 Msa1013950:CDS 35.0%
ACCTGTTCTCATTGTTTGTA+AGG + chr7_1:21461658-21461677 None:intergenic 35.0%
! TTCATCTTTTATAGAGTCCG+AGG - chr7_1:21461740-21461759 Msa1013950:CDS 35.0%
! TCATCTTTTATAGAGTCCGA+GGG - chr7_1:21461741-21461760 Msa1013950:CDS 35.0%
TTGCAGATTGGACTAAAAGA+TGG - chr7_1:21461806-21461825 Msa1013950:CDS 35.0%
! TTTTCTGCAGAACACTATGA+AGG - chr7_1:21462148-21462167 Msa1013950:intron 35.0%
! TTTGTCATCTAGTTCAGTCA+TGG + chr7_1:21462568-21462587 None:intergenic 35.0%
TCATAATCGCCTAAGCCTAA+TGG + chr7_1:21460076-21460095 None:intergenic 40.0%
ATTAGGCTTAGGCGATTATG+AGG - chr7_1:21460075-21460094 Msa1013950:three_prime_UTR 40.0%
GAGTCCAAATAAGACTGGAA+GGG + chr7_1:21460176-21460195 None:intergenic 40.0%
AGAGTCCAAATAAGACTGGA+AGG + chr7_1:21460177-21460196 None:intergenic 40.0%
!!! CCCTTCAACGTGGTTTTAAT+TGG - chr7_1:21460329-21460348 Msa1013950:three_prime_UTR 40.0%
TCATAGTCAGCAGAAGAACA+AGG - chr7_1:21460450-21460469 Msa1013950:three_prime_UTR 40.0%
GAGTCAAGAAAGTTGCCAAT+AGG + chr7_1:21460498-21460517 None:intergenic 40.0%
CGGCTGCTGTAAAATTAGAA+TGG + chr7_1:21460733-21460752 None:intergenic 40.0%
GTTTGGTTGTAAGTTCTTCG+AGG + chr7_1:21460758-21460777 None:intergenic 40.0%
! ATGTCGAAGCATTTCGTGTT+TGG + chr7_1:21460775-21460794 None:intergenic 40.0%
TGTGAAAGACATGGCTTCTT+GGG + chr7_1:21460805-21460824 None:intergenic 40.0%
TTGTGAAAGACATGGCTTCT+TGG + chr7_1:21460806-21460825 None:intergenic 40.0%
GTGTCAAAAGAAATGACTCG+GGG - chr7_1:21460929-21460948 Msa1013950:intron 40.0%
CCTTAACAACTGATGATTCC+GGG + chr7_1:21460982-21461001 None:intergenic 40.0%
GAAGCTAAATCAAGTGAGAC+AGG - chr7_1:21461190-21461209 Msa1013950:three_prime_UTR 40.0%
AATGCAAGTGGCCAATTTGT+TGG - chr7_1:21461497-21461516 Msa1013950:CDS 40.0%
TCTCTGCTACTCCAACAAAT+TGG + chr7_1:21461511-21461530 None:intergenic 40.0%
ACGGATTCTTCCCAATTAAG+TGG - chr7_1:21461588-21461607 Msa1013950:CDS 40.0%
GATGTGTCGAAACAAACAGT+TGG + chr7_1:21461633-21461652 None:intergenic 40.0%
ACAGGTGTGTAACTTTCAGT+AGG - chr7_1:21461672-21461691 Msa1013950:CDS 40.0%
CAGGTGTGTAACTTTCAGTA+GGG - chr7_1:21461673-21461692 Msa1013950:CDS 40.0%
GATTGGACTAAAAGATGGTC+TGG - chr7_1:21461811-21461830 Msa1013950:CDS 40.0%
TCAGGCAAAGACATCTCTAT+TGG - chr7_1:21461859-21461878 Msa1013950:CDS 40.0%
! AGAAGTTATTTCGTTCCCAG+AGG - chr7_1:21461909-21461928 Msa1013950:CDS 40.0%
GAGAAGCTGATAAGTAGCTA+CGG + chr7_1:21462042-21462061 None:intergenic 40.0%
TTTGATCAGCGTCTCATGAT+TGG + chr7_1:21462213-21462232 None:intergenic 40.0%
AGACGCTGATCAAACTAACA+AGG - chr7_1:21462219-21462238 Msa1013950:CDS 40.0%
CTAGTTCAGTCATGGTTGAA+TGG + chr7_1:21462560-21462579 None:intergenic 40.0%
GGTTCTTAAGTTCGGCCATT+AGG - chr7_1:21460058-21460077 Msa1013950:three_prime_UTR 45.0%
TAAGTTCGGCCATTAGGCTT+AGG - chr7_1:21460064-21460083 Msa1013950:three_prime_UTR 45.0%
AGGCGATTATGAGGAGGATA+CGG - chr7_1:21460084-21460103 Msa1013950:three_prime_UTR 45.0%
TCCTTTCAGGCTTCATGCTT+GGG + chr7_1:21460126-21460145 None:intergenic 45.0%
ATCCTTTCAGGCTTCATGCT+TGG + chr7_1:21460127-21460146 None:intergenic 45.0%
AAGCATGAAGCCTGAAAGGA+TGG - chr7_1:21460126-21460145 Msa1013950:three_prime_UTR 45.0%
!! CATGCCCTTCCAGTCTTATT+TGG - chr7_1:21460169-21460188 Msa1013950:three_prime_UTR 45.0%
AATCAGTAGACCCTTCAACG+TGG - chr7_1:21460319-21460338 Msa1013950:three_prime_UTR 45.0%
AACAAGGCAGATGTGGTATG+TGG - chr7_1:21460466-21460485 Msa1013950:three_prime_UTR 45.0%
GTTGTAAGTTCTTCGAGGGT+CGG + chr7_1:21460753-21460772 None:intergenic 45.0%
AAAGACATGGCTTCTTGGGT+TGG + chr7_1:21460801-21460820 None:intergenic 45.0%
!! CTGTAGTTAGTAGAGGCACT+TGG + chr7_1:21460842-21460861 None:intergenic 45.0%
AACAACTGATGATTCCGGGT+TGG + chr7_1:21460978-21460997 None:intergenic 45.0%
CCCGGAATCATCAGTTGTTA+AGG - chr7_1:21460979-21460998 Msa1013950:three_prime_UTR 45.0%
CCGAGAAGAAGAGGAAGATT+GGG + chr7_1:21461225-21461244 None:intergenic 45.0%
CCCAATCTTCCTCTTCTTCT+CGG - chr7_1:21461222-21461241 Msa1013950:three_prime_UTR 45.0%
ACCGAGAAGAAGAGGAAGAT+TGG + chr7_1:21461226-21461245 None:intergenic 45.0%
TTGAACTCACCGAGAAGAAG+AGG + chr7_1:21461234-21461253 None:intergenic 45.0%
TTGGAGTAGCAGAGATGCTA+GGG - chr7_1:21461516-21461535 Msa1013950:CDS 45.0%
TTTCAGTAGGGACACACAAG+AGG - chr7_1:21461685-21461704 Msa1013950:CDS 45.0%
TACGCGACAACTATATCCCT+CGG + chr7_1:21461760-21461779 None:intergenic 45.0%
! CGACACTCTTGTTTGCAGAT+TGG - chr7_1:21461794-21461813 Msa1013950:CDS 45.0%
CTAAAGCCATGTCTTCCAGA+GGG + chr7_1:21462260-21462279 None:intergenic 45.0%
! TCTAAAGCCATGTCTTCCAG+AGG + chr7_1:21462261-21462280 None:intergenic 45.0%
AGGCTTAGGCGATTATGAGG+AGG - chr7_1:21460078-21460097 Msa1013950:three_prime_UTR 50.0%
GGCGATTATGAGGAGGATAC+GGG - chr7_1:21460085-21460104 Msa1013950:three_prime_UTR 50.0%
CTTTCAGGCTTCATGCTTGG+GGG + chr7_1:21460124-21460143 None:intergenic 50.0%
CCTTTCAGGCTTCATGCTTG+GGG + chr7_1:21460125-21460144 None:intergenic 50.0%
GCAGAAGAACAAGGCAGATG+TGG - chr7_1:21460459-21460478 Msa1013950:three_prime_UTR 50.0%
GGTATGTGGTGACTGCCTAT+TGG - chr7_1:21460480-21460499 Msa1013950:three_prime_UTR 50.0%
! AAGAAATGACTCGGGGTCCT+CGG - chr7_1:21460936-21460955 Msa1013950:intron 50.0%
GTTTGGACAAGCACTCCACA+TGG - chr7_1:21461133-21461152 Msa1013950:three_prime_UTR 50.0%
GTTGGAGTAGCAGAGATGCT+AGG - chr7_1:21461515-21461534 Msa1013950:CDS 50.0%
ATGCTGCTCGGTAATCCTCT+GGG + chr7_1:21461927-21461946 None:intergenic 50.0%
TATGCTGCTCGGTAATCCTC+TGG + chr7_1:21461928-21461947 None:intergenic 50.0%
AGGATTACCGAGCAGCATAC+AGG - chr7_1:21461929-21461948 Msa1013950:intron 50.0%
GATCAGCGTCTCATGATTGG+TGG + chr7_1:21462210-21462229 None:intergenic 50.0%
AATCTCTCTCTCACTCCCTC+TGG - chr7_1:21462242-21462261 Msa1013950:intron 50.0%
TGCTTGGGGGTCTATGGAGT+CGG + chr7_1:21460111-21460130 None:intergenic 55.0%
GCTTCATGCTTGGGGGTCTA+TGG + chr7_1:21460117-21460136 None:intergenic 55.0%
CCCCAAGCATGAAGCCTGAA+AGG - chr7_1:21460122-21460141 Msa1013950:three_prime_UTR 55.0%
AGTAGCAGAGATGCTAGGGC+CGG - chr7_1:21461520-21461539 Msa1013950:CDS 55.0%
TGTCGTGCCTGTATGCTGCT+CGG + chr7_1:21461939-21461958 None:intergenic 55.0%
! CTCACTCCCTCTGGAAGACA+TGG - chr7_1:21462251-21462270 Msa1013950:intron 55.0%
AGCAGAGATGCTAGGGCCGG+TGG - chr7_1:21461523-21461542 Msa1013950:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 21460055 21462608 21460055 ID=Msa1013950;Name=Msa1013950
chr7_1 mRNA 21460055 21462608 21460055 ID=Msa1013950-mRNA-1;Parent=Msa1013950;Name=Msa1013950-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|0.75|0.8|1|1|0.8|5|1019|219
chr7_1 exon 21460055 21460505 21460055 ID=Msa1013950-mRNA-1:exon:5134;Parent=Msa1013950-mRNA-1
chr7_1 exon 21460675 21460851 21460675 ID=Msa1013950-mRNA-1:exon:5133;Parent=Msa1013950-mRNA-1
chr7_1 exon 21460957 21461944 21460957 ID=Msa1013950-mRNA-1:exon:5132;Parent=Msa1013950-mRNA-1
chr7_1 exon 21462183 21462241 21462183 ID=Msa1013950-mRNA-1:exon:5131;Parent=Msa1013950-mRNA-1
chr7_1 exon 21462605 21462608 21462605 ID=Msa1013950-mRNA-1:exon:5130;Parent=Msa1013950-mRNA-1
chr7_1 CDS 21462605 21462608 21462605 ID=Msa1013950-mRNA-1:cds;Parent=Msa1013950-mRNA-1
chr7_1 CDS 21462183 21462241 21462183 ID=Msa1013950-mRNA-1:cds;Parent=Msa1013950-mRNA-1
chr7_1 CDS 21461348 21461944 21461348 ID=Msa1013950-mRNA-1:cds;Parent=Msa1013950-mRNA-1
chr7_1 three_prime_UTR 21460957 21461347 21460957 ID=Msa1013950-mRNA-1:three_prime_utr;Parent=Msa1013950-mRNA-1
chr7_1 three_prime_UTR 21460675 21460851 21460675 ID=Msa1013950-mRNA-1:three_prime_utr;Parent=Msa1013950-mRNA-1
chr7_1 three_prime_UTR 21460055 21460505 21460055 ID=Msa1013950-mRNA-1:three_prime_utr;Parent=Msa1013950-mRNA-1
Gene Sequence

>Msa1013950

ATGGCTCAATCTATTCAAACAGCAACTACAAATCATAGTCAGCAGAAGAACAAGGCAGATGTGGCTCCTCGTTTCCATTCTAATTTTACAGCAGCCGACCCTCGAAGAACTTACAACCAAACACGAAATGCTTCGACATTTGCCAACCCAAGAAGCCATGTCTTTCACAATACTAATTACAGGCCAAGTGCCTCTACTAACTACAGACCAAATGGTATAGTATCTAATGATAAAGTTTTGTTAAGTGAAAAATTCAGAAGTGAAGAATCTGAAGTGTCAAAAGAAATGACTCGGGGTCCTCGGTTTCATCAAAACAATTCCAACCCGGAATCATCAGTTGTTAAGGATGAATTTGCGTTCACAGTGTGCAGAGATCGATATAACCTTCCAGATTTCCAAACTAAATATGAAACTGCTAAATTTTATATGATTAAATCTTTCAATGAAGATCACATTCAAAAGGGAATAAATAATGATGTTTGGACAAGCACTCCACATGGAAATAAGAAGTTGAACGCTGCATTCCAAAACGCAGAAGCTAAATCAAGTGAGACAGGCACACAGTGCCCAATCTTCCTCTTCTTCTCGGTGAGTTCAAATCTTTTCTCCAAGTTTTCGCTTGCCATTTTTTTAATACAAGATGGCATTATAGAAACTTAA
Protein sequence

>Msa1013950

MAQSIQTATTNHSQQKNKADVAPRFHSNFTAADPRRTYNQTRNASTFANPRSHVFHNTNYRPSASTNYRPNGIVSNDKVLLSEKFRSEESEVSKEMTRGPRFHQNNSNPESSVVKDEFAFTVCRDRYNLPDFQTKYETAKFYMIKSFNEDHIQKGINNDVWTSTPHGNKKLNAAFQNAEAKSSETGTQCPIFLFFSVSSNLFSKFSLAIFLIQDGIIET*