AlfalfaGEDB | Msa1023330

M. sativa cultivar zhongmu-4 / Msa1023330

KEGG: K14686
GO: GO:0000003 GO:0000041 GO:0003006 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0007275 GO:0007276 GO:0008150 GO:0008324 GO:0009555 GO:0009987 GO:0015075 GO:0015089 GO:0015318 GO:0015677 GO:0015679 GO:0016020 GO:0019953 GO:0022412 GO:0022414 GO:0022622 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0034220 GO:0035434 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0046658 GO:0046873 GO:0046915 GO:0048229 GO:0048232 GO:0048235 GO:0048364 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051704 GO:0055046 GO:0055085 GO:0071944 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098705 GO:0098739 GO:0099402 GO:0099587
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023330	XP_013443255.1	94.631	149	8	0	1	149	1	149	1.09e-96	285

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023330	sp\|Q8GWP3\|COPT6_ARATH	44.961	129	69	1	12	140	10	136	2.10e-32	115

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023330	A0A072TUQ5	94.631	149	8	0	1	149	1	149	5.22e-97	285

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0029960	Msa1023330	0.800908	1.155619e-48	-8.615850e-47
Msa0155180	Msa1023330	0.810615	1.055577e-50	-8.615850e-47
Msa1023330	Msa1061900	0.847627	1.026025e-59	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023330	MtrunA17_Chr7g0240861	94.631	149	8	0	1	149	1	149	1.01e-100	285
Msa1023330	MtrunA17_Chr4g0032291	55.446	101	42	2	30	130	46	143	9.56e-28	100
Msa1023330	MtrunA17_Chr4g0032581	53.535	99	43	2	30	128	35	130	1.75e-27	100
Msa1023330	MtrunA17_Chr4g0032571	48.077	104	50	2	27	130	28	127	5.72e-26	95.9
Msa1023330	MtrunA17_Chr7g0240831	45.217	115	60	2	27	141	22	133	6.17e-25	93.2
Msa1023330	MtrunA17_Chr7g0240841	45.217	115	60	2	27	141	22	133	6.17e-25	93.2
Msa1023330	MtrunA17_Chr4g0008841	41.270	126	71	2	22	147	8	130	2.88e-24	91.3
Msa1023330	MtrunA17_Chr1g0150721	31.655	139	76	3	27	147	2	139	5.15e-16	70.1
Msa1023330	MtrunA17_Chr3g0136431	32.143	140	66	4	27	145	2	133	1.29e-14	66.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1023330	AT2G26975.1	44.961	129	69	1	12	140	10	136	2.13e-33	115
Msa1023330	AT5G59030.1	45.000	120	66	0	30	149	45	164	3.86e-33	115
Msa1023330	AT2G37925.1	47.826	115	58	2	27	140	30	143	2.26e-31	110
Msa1023330	AT3G46900.1	47.273	110	56	2	31	140	34	141	1.31e-30	108
Msa1023330	AT5G59040.1	48.246	114	56	1	27	140	30	140	2.30e-28	102
Msa1023330	AT5G20650.1	34.028	144	68	4	27	148	2	140	2.01e-15	68.9

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGCCATGTTATTGGGTTTA+TGG	0.163011	7_1:-50722826	Msa1023330:CDS
GCAGTTGGTGGCCATGTTAT+TGG	0.206160	7_1:-50722834	Msa1023330:CDS
CGATCCACACATCATTCTAT+TGG	0.284405	7_1:-50723115	Msa1023330:CDS
TTGGCTGTTATGTCGTTTAA+TGG	0.294096	7_1:-50722873	Msa1023330:CDS
CAGTTGGTGGCCATGTTATT+GGG	0.316028	7_1:-50722833	Msa1023330:CDS
GATCCACACATCATTCTATT+GGG	0.329197	7_1:-50723114	Msa1023330:CDS
TCCACGGTGGCGACGATGTT+GGG	0.338831	7_1:-50723152	Msa1023330:CDS
GGGACATCAAACGAAGTCTT+TGG	0.399658	7_1:-50722954	Msa1023330:CDS
TACTATTCCATTGCTGGCCC+GGG	0.418443	7_1:-50723076	Msa1023330:CDS
TAGACATACTATTCCATTGC+TGG	0.428991	7_1:-50723082	Msa1023330:CDS
CTCCACGGTGGCGACGATGT+TGG	0.436829	7_1:-50723153	Msa1023330:CDS
ATACTATTCCATTGCTGGCC+CGG	0.443048	7_1:-50723077	Msa1023330:CDS
AAAGACTTCGTTTGATGTCC+CGG	0.445790	7_1:+50722956	None:intergenic
CGCTGTCCCCGGGCCAGCAA+TGG	0.456744	7_1:+50723069	None:intergenic
CCGTACTGGATTCTCGTACT+TGG	0.459305	7_1:-50722901	Msa1023330:CDS
CATCGTCGCCACCGTGGAGT+TGG	0.477324	7_1:+50723157	None:intergenic
GTACATGACAGCGCTGTCCC+CGG	0.489634	7_1:+50723058	None:intergenic
ATTCTCGTACTTGGTTATGT+TGG	0.491065	7_1:-50722892	Msa1023330:CDS
GGGCTGTACGGAGTCCGTAC+TGG	0.494095	7_1:-50722915	Msa1023330:CDS
GTGTTTCTTGCTGCAGTTGG+TGG	0.500610	7_1:-50722846	Msa1023330:CDS
GGTGTGTTTCTTGCTGCAGT+TGG	0.502035	7_1:-50722849	Msa1023330:CDS
GGGTTTGAGTTTAACAATGT+TGG	0.502334	7_1:+50722977	None:intergenic
GTTTGTGATGTCGGTGTTGG+TGG	0.509208	7_1:-50723015	Msa1023330:CDS
TTGTTCACCACCACTGCAAA+TGG	0.515915	7_1:-50723188	Msa1023330:CDS
CGTGTTTGTGATGTCGGTGT+TGG	0.516487	7_1:-50723018	Msa1023330:CDS
AAGACTTCGTTTGATGTCCC+GGG	0.535430	7_1:+50722957	None:intergenic
TACATGACAGCGCTGTCCCC+GGG	0.548116	7_1:+50723059	None:intergenic
CGGTGGCGACGATGTTGGGC+CGG	0.564945	7_1:-50723148	Msa1023330:CDS
TGTGGATCGGAATTCTTCGC+CGG	0.568160	7_1:+50723129	None:intergenic
GATCCTCGTGTTTGTGATGT+CGG	0.571906	7_1:-50723024	Msa1023330:CDS
AACATTGTTAAACTCAAACC+CGG	0.572414	7_1:-50722975	Msa1023330:CDS
TGATGTCGGTGTTGGTGGAG+TGG	0.577541	7_1:-50723010	Msa1023330:CDS
CAATAGAATGATGTGTGGAT+CGG	0.613976	7_1:+50723116	None:intergenic
AGGAACACCATTTGCAGTGG+TGG	0.618142	7_1:+50723181	None:intergenic
GCTGTTATGTCGTTTAATGG+TGG	0.619102	7_1:-50722870	Msa1023330:CDS
GTCGCCACCGTGGAGTTGGA+AGG	0.621088	7_1:+50723161	None:intergenic
TGGTGTTCCTTCCAACTCCA+CGG	0.621560	7_1:-50723168	Msa1023330:CDS
GGAAGGAACACCATTTGCAG+TGG	0.632915	7_1:+50723178	None:intergenic
ACATTGTTAAACTCAAACCC+GGG	0.639732	7_1:-50722974	Msa1023330:CDS
ACTATTCCATTGCTGGCCCG+GGG	0.640157	7_1:-50723075	Msa1023330:CDS
ATCCACACATCATTCTATTG+GGG	0.654547	7_1:-50723113	Msa1023330:CDS
AAACCATAAACCCAATAACA+TGG	0.667055	7_1:+50722823	None:intergenic
GACCCCAATAGAATGATGTG+TGG	0.683603	7_1:+50723111	None:intergenic
GCCCAACATCGTCGCCACCG+TGG	0.693359	7_1:+50723151	None:intergenic
CCAAGTACGAGAATCCAGTA+CGG	0.693631	7_1:+50722901	None:intergenic
ACACCGACATCACAAACACG+AGG	0.739798	7_1:+50723021	None:intergenic
TGTTCCTTCCAACTCCACGG+TGG	0.776714	7_1:-50723165	Msa1023330:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATTGTTAAACTCAAACC+CGG	-	chr7_1:50722980-50722999	Msa1023330:CDS	30.0%
	AAACCATAAACCCAATAACA+TGG	+	chr7_1:50723135-50723154	None:intergenic	30.0%
!	GTTATTGGGTTTATGGTTTT+TGG	-	chr7_1:50723136-50723155	Msa1023330:CDS	30.0%
!!	ATGGCTATTTTGAAGAAATC+TGG	-	chr7_1:50723163-50723182	Msa1023330:CDS	30.0%
!!	CTATTTTGAAGAAATCTGGT+GGG	-	chr7_1:50723167-50723186	Msa1023330:CDS	30.0%
	CAATAGAATGATGTGTGGAT+CGG	+	chr7_1:50722842-50722861	None:intergenic	35.0%
	GATCCACACATCATTCTATT+GGG	-	chr7_1:50722841-50722860	Msa1023330:CDS	35.0%
	ATCCACACATCATTCTATTG+GGG	-	chr7_1:50722842-50722861	Msa1023330:CDS	35.0%
	TAGACATACTATTCCATTGC+TGG	-	chr7_1:50722873-50722892	Msa1023330:CDS	35.0%
!	GGGTTTGAGTTTAACAATGT+TGG	+	chr7_1:50722981-50723000	None:intergenic	35.0%
	ACATTGTTAAACTCAAACCC+GGG	-	chr7_1:50722981-50723000	Msa1023330:CDS	35.0%
!!	AAGTCTTTGGCTCACTTTTA+AGG	-	chr7_1:50723014-50723033	Msa1023330:CDS	35.0%
	ATTCTCGTACTTGGTTATGT+TGG	-	chr7_1:50723063-50723082	Msa1023330:CDS	35.0%
!	TTGGCTGTTATGTCGTTTAA+TGG	-	chr7_1:50723082-50723101	Msa1023330:CDS	35.0%
!!!	GTTTATGGTTTTTGGTACGA+TGG	-	chr7_1:50723144-50723163	Msa1023330:CDS	35.0%
!!	GCTATTTTGAAGAAATCTGG+TGG	-	chr7_1:50723166-50723185	Msa1023330:CDS	35.0%
	CGATCCACACATCATTCTAT+TGG	-	chr7_1:50722840-50722859	Msa1023330:CDS	40.0%
	AAAGACTTCGTTTGATGTCC+CGG	+	chr7_1:50723002-50723021	None:intergenic	40.0%
!!	CTTTGGCTCACTTTTAAGGA+CGG	-	chr7_1:50723018-50723037	Msa1023330:CDS	40.0%
!!	TTTGGCTCACTTTTAAGGAC+GGG	-	chr7_1:50723019-50723038	Msa1023330:CDS	40.0%
!	GCTGTTATGTCGTTTAATGG+TGG	-	chr7_1:50723085-50723104	Msa1023330:CDS	40.0%
	TGGCCATGTTATTGGGTTTA+TGG	-	chr7_1:50723129-50723148	Msa1023330:CDS	40.0%
	TTGTTCACCACCACTGCAAA+TGG	-	chr7_1:50722767-50722786	Msa1023330:CDS	45.0%
	GACCCCAATAGAATGATGTG+TGG	+	chr7_1:50722847-50722866	None:intergenic	45.0%
	ATACTATTCCATTGCTGGCC+CGG	-	chr7_1:50722878-50722897	Msa1023330:CDS	45.0%
	GATCCTCGTGTTTGTGATGT+CGG	-	chr7_1:50722931-50722950	Msa1023330:CDS	45.0%
	AAGACTTCGTTTGATGTCCC+GGG	+	chr7_1:50723001-50723020	None:intergenic	45.0%
	GGGACATCAAACGAAGTCTT+TGG	-	chr7_1:50723001-50723020	Msa1023330:CDS	45.0%
!!	TTGGCTCACTTTTAAGGACG+GGG	-	chr7_1:50723020-50723039	Msa1023330:CDS	45.0%
	CCAAGTACGAGAATCCAGTA+CGG	+	chr7_1:50723057-50723076	None:intergenic	45.0%
!!	CAGTTGGTGGCCATGTTATT+GGG	-	chr7_1:50723122-50723141	Msa1023330:CDS	45.0%
	AGGAACACCATTTGCAGTGG+TGG	+	chr7_1:50722777-50722796	None:intergenic	50.0%
	GGAAGGAACACCATTTGCAG+TGG	+	chr7_1:50722780-50722799	None:intergenic	50.0%
	TGGTGTTCCTTCCAACTCCA+CGG	-	chr7_1:50722787-50722806	Msa1023330:CDS	50.0%
	TGTGGATCGGAATTCTTCGC+CGG	+	chr7_1:50722829-50722848	None:intergenic	50.0%
	TACTATTCCATTGCTGGCCC+GGG	-	chr7_1:50722879-50722898	Msa1023330:CDS	50.0%
	ACACCGACATCACAAACACG+AGG	+	chr7_1:50722937-50722956	None:intergenic	50.0%
	CGTGTTTGTGATGTCGGTGT+TGG	-	chr7_1:50722937-50722956	Msa1023330:CDS	50.0%
!!	GTTTGTGATGTCGGTGTTGG+TGG	-	chr7_1:50722940-50722959	Msa1023330:CDS	50.0%
!!	CTTTTAAGGACGGGGCTGTA+CGG	-	chr7_1:50723028-50723047	Msa1023330:CDS	50.0%
	CCGTACTGGATTCTCGTACT+TGG	-	chr7_1:50723054-50723073	Msa1023330:CDS	50.0%
	GGTGTGTTTCTTGCTGCAGT+TGG	-	chr7_1:50723106-50723125	Msa1023330:CDS	50.0%
	GTGTTTCTTGCTGCAGTTGG+TGG	-	chr7_1:50723109-50723128	Msa1023330:CDS	50.0%
!!	GCAGTTGGTGGCCATGTTAT+TGG	-	chr7_1:50723121-50723140	Msa1023330:CDS	50.0%
	TGTTCCTTCCAACTCCACGG+TGG	-	chr7_1:50722790-50722809	Msa1023330:CDS	55.0%
	ACTATTCCATTGCTGGCCCG+GGG	-	chr7_1:50722880-50722899	Msa1023330:CDS	55.0%
!!	TGATGTCGGTGTTGGTGGAG+TGG	-	chr7_1:50722945-50722964	Msa1023330:CDS	55.0%
	TCCACGGTGGCGACGATGTT+GGG	-	chr7_1:50722803-50722822	Msa1023330:CDS	60.0%
	TACATGACAGCGCTGTCCCC+GGG	+	chr7_1:50722899-50722918	None:intergenic	60.0%
	GTACATGACAGCGCTGTCCC+CGG	+	chr7_1:50722900-50722919	None:intergenic	60.0%
	GTCGCCACCGTGGAGTTGGA+AGG	+	chr7_1:50722797-50722816	None:intergenic	65.0%
	CATCGTCGCCACCGTGGAGT+TGG	+	chr7_1:50722801-50722820	None:intergenic	65.0%
	CTCCACGGTGGCGACGATGT+TGG	-	chr7_1:50722802-50722821	Msa1023330:CDS	65.0%
	GGGCTGTACGGAGTCCGTAC+TGG	-	chr7_1:50723040-50723059	Msa1023330:CDS	65.0%
	GCCCAACATCGTCGCCACCG+TGG	+	chr7_1:50722807-50722826	None:intergenic	70.0%
	CGGTGGCGACGATGTTGGGC+CGG	-	chr7_1:50722807-50722826	Msa1023330:CDS	70.0%
	CGCTGTCCCCGGGCCAGCAA+TGG	+	chr7_1:50722889-50722908	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	50722764	50723213	50722764	ID=Msa1023330;Name=Msa1023330
chr7_1	mRNA	50722764	50723213	50722764	ID=Msa1023330-mRNA-1;Parent=Msa1023330;Name=Msa1023330-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|149
chr7_1	exon	50722764	50723213	50722764	ID=Msa1023330-mRNA-1:exon:9957;Parent=Msa1023330-mRNA-1
chr7_1	CDS	50722764	50723213	50722764	ID=Msa1023330-mRNA-1:cds;Parent=Msa1023330-mRNA-1

Gene Sequence

>Msa1023330

ATGTTGTTCACCACCACTGCAAATGGTGTTCCTTCCAACTCCACGGTGGCGACGATGTTGGGCCGGCGAAGAATTCCGATCCACACATCATTCTATTGGGGTCATAATGTAGACATACTATTCCATTGCTGGCCCGGGGACAGCGCTGTCATGTACGCAGTAGCGCTGATCCTCGTGTTTGTGATGTCGGTGTTGGTGGAGTGGCTTTCATTTACCAACATTGTTAAACTCAAACCCGGGACATCAAACGAAGTCTTTGGCTCACTTTTAAGGACGGGGCTGTACGGAGTCCGTACTGGATTCTCGTACTTGGTTATGTTGGCTGTTATGTCGTTTAATGGTGGTGTGTTTCTTGCTGCAGTTGGTGGCCATGTTATTGGGTTTATGGTTTTTGGTACGATGGCTATTTTGAAGAAATCTGGTGGGTTAGATTCTTCTTCTAAACCATAG

Protein sequence

>Msa1023330

MLFTTTANGVPSNSTVATMLGRRRIPIHTSFYWGHNVDILFHCWPGDSAVMYAVALILVFVMSVLVEWLSFTNIVKLKPGTSNEVFGSLLRTGLYGVRTGFSYLVMLAVMSFNGGVFLAAVGGHVIGFMVFGTMAILKKSGGLDSSSKP*