AlfalfaGEDB | Msa1061900

M. sativa cultivar zhongmu-4 / Msa1061900

KEGG: K14686
GO: GO:0000003 GO:0000041 GO:0003006 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0007275 GO:0007276 GO:0008150 GO:0008324 GO:0009555 GO:0009987 GO:0015075 GO:0015089 GO:0015318 GO:0015677 GO:0015679 GO:0016020 GO:0019953 GO:0022412 GO:0022414 GO:0022622 GO:0022857 GO:0022890 GO:0030001 GO:0030154 GO:0031224 GO:0031225 GO:0031226 GO:0032501 GO:0032502 GO:0032504 GO:0034220 GO:0035434 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0046658 GO:0046873 GO:0046915 GO:0048229 GO:0048232 GO:0048235 GO:0048364 GO:0048609 GO:0048731 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051704 GO:0055046 GO:0055085 GO:0071944 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098705 GO:0098739 GO:0099402 GO:0099587
PFAM: PF04145.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061900	XP_013443255.1	95.302	149	7	0	1	149	1	149	1.57e-97	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061900	sp\|Q39065\|COPT1_ARATH	45.000	120	66	0	30	149	45	164	1.09e-32	116

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061900	A0A072TUQ5	95.302	149	7	0	1	149	1	149	7.49e-98	287

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0029990	Msa1061900	0.801101	1.055510e-48	-8.615850e-47
Msa0030070	Msa1061900	0.807881	4.070282e-50	-8.615850e-47
Msa0071840	Msa1061900	0.813386	2.627527e-51	-8.615850e-47
Msa0100600	Msa1061900	0.804664	1.938129e-49	-8.615850e-47
Msa0657210	Msa1061900	0.816283	5.988913e-52	-8.615850e-47
Msa0701250	Msa1061900	0.818502	1.895455e-52	-8.615850e-47
Msa0125590	Msa1061900	0.812008	5.262318e-51	-8.615850e-47
Msa1023330	Msa1061900	0.847627	1.026025e-59	-8.615850e-47
Msa1061900	Msa1267930	0.819426	1.168989e-52	-8.615850e-47
Msa0541740	Msa1061900	0.819296	1.251452e-52	-8.615850e-47
Msa0876630	Msa1061900	0.801975	6.985057e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061900	MtrunA17_Chr7g0240861	95.302	149	7	0	1	149	1	149	1.44e-101	287
Msa1061900	MtrunA17_Chr4g0032581	54.545	99	42	2	30	128	35	130	2.46e-28	102
Msa1061900	MtrunA17_Chr4g0032291	55.446	101	42	2	30	130	46	143	3.14e-28	102
Msa1061900	MtrunA17_Chr4g0032571	49.038	104	49	2	27	130	28	127	7.07e-27	98.2
Msa1061900	MtrunA17_Chr7g0240831	45.217	115	60	2	27	141	22	133	3.55e-25	94.0
Msa1061900	MtrunA17_Chr7g0240841	45.217	115	60	2	27	141	22	133	3.55e-25	94.0
Msa1061900	MtrunA17_Chr4g0008841	41.270	126	71	2	22	147	8	130	1.50e-24	92.0
Msa1061900	MtrunA17_Chr1g0150721	31.690	142	72	2	27	147	2	139	2.31e-16	71.2
Msa1061900	MtrunA17_Chr3g0136431	31.429	140	67	4	27	145	2	133	2.10e-14	65.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1061900	AT5G59030.1	45.000	120	66	0	30	149	45	164	1.11e-33	116
Msa1061900	AT2G26975.1	44.961	129	69	1	12	140	10	136	2.02e-33	115
Msa1061900	AT2G37925.1	47.826	115	58	2	27	140	30	143	5.62e-32	111
Msa1061900	AT3G46900.1	47.273	110	56	2	31	140	34	141	4.74e-31	109
Msa1061900	AT5G59040.1	48.246	114	56	1	27	140	30	140	1.52e-28	102
Msa1061900	AT5G20650.1	34.028	144	68	4	27	148	2	140	1.17e-15	69.7

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGCCATGTTATTGGGTTTA+TGG	0.163011	7_2:+52355935	Msa1061900:CDS
GCAGTTGGTGGCCATGTTAT+TGG	0.206160	7_2:+52355927	Msa1061900:CDS
CGATCCACACATCATTCTAT+TGG	0.284405	7_2:+52355646	Msa1061900:CDS
TTGGCTGTTATGTCGTTTAA+TGG	0.294096	7_2:+52355888	Msa1061900:CDS
CAGTTGGTGGCCATGTTATT+GGG	0.316028	7_2:+52355928	Msa1061900:CDS
GATCCACACATCATTCTATT+GGG	0.329197	7_2:+52355647	Msa1061900:CDS
TCCACGGTGGCGACGATGTT+GGG	0.338831	7_2:+52355609	Msa1061900:CDS
TACTATTCCATTGCTGGCCC+GGG	0.418443	7_2:+52355685	Msa1061900:CDS
AACGACTTCGTTTGATGTCC+CGG	0.419471	7_2:-52355805	None:intergenic
TAGACATACTATTCCATTGC+TGG	0.428991	7_2:+52355679	Msa1061900:CDS
CTCCACGGTGGCGACGATGT+TGG	0.436829	7_2:+52355608	Msa1061900:CDS
ATACTATTCCATTGCTGGCC+CGG	0.443048	7_2:+52355684	Msa1061900:CDS
CGCTGTCCCCGGGCCAGCAA+TGG	0.456744	7_2:-52355692	None:intergenic
CCGTACTGGATTCTCGTACT+TGG	0.459305	7_2:+52355860	Msa1061900:CDS
CATCGTCGCCACCGTGGAGT+TGG	0.477324	7_2:-52355604	None:intergenic
GTACATGACAGCGCTGTCCC+CGG	0.489634	7_2:-52355703	None:intergenic
ATTCTCGTACTTGGTTATGT+TGG	0.491065	7_2:+52355869	Msa1061900:CDS
GGGCTGTACGGAGTCCGTAC+TGG	0.494095	7_2:+52355846	Msa1061900:CDS
GTGTTTCTTGCTGCAGTTGG+TGG	0.500610	7_2:+52355915	Msa1061900:CDS
GGTGTGTTTCTTGCTGCAGT+TGG	0.502035	7_2:+52355912	Msa1061900:CDS
GGGTTTGAGTTTAACAATGT+TGG	0.502334	7_2:-52355784	None:intergenic
GTTTGTGATGTCGGTGTTGG+TGG	0.509208	7_2:+52355746	Msa1061900:CDS
TTGTTCACCACCACTGCAAA+TGG	0.515915	7_2:+52355573	Msa1061900:CDS
CGTGTTTGTGATGTCGGTGT+TGG	0.516487	7_2:+52355743	Msa1061900:CDS
ACGACTTCGTTTGATGTCCC+GGG	0.531302	7_2:-52355804	None:intergenic
TACATGACAGCGCTGTCCCC+GGG	0.548116	7_2:-52355702	None:intergenic
CGGTGGCGACGATGTTGGGC+CGG	0.564945	7_2:+52355613	Msa1061900:CDS
TGTGGATCGGAATTCTTCGC+CGG	0.568160	7_2:-52355632	None:intergenic
GATCCTCGTGTTTGTGATGT+CGG	0.571906	7_2:+52355737	Msa1061900:CDS
AACATTGTTAAACTCAAACC+CGG	0.572414	7_2:+52355786	Msa1061900:CDS
TGATGTCGGTGTTGGTGGAG+TGG	0.577541	7_2:+52355751	Msa1061900:CDS
CAATAGAATGATGTGTGGAT+CGG	0.613976	7_2:-52355645	None:intergenic
AGGAACACCATTTGCAGTGG+TGG	0.618142	7_2:-52355580	None:intergenic
GCTGTTATGTCGTTTAATGG+TGG	0.619102	7_2:+52355891	Msa1061900:CDS
GTCGCCACCGTGGAGTTGGA+AGG	0.621088	7_2:-52355600	None:intergenic
TGGTGTTCCTTCCAACTCCA+CGG	0.621560	7_2:+52355593	Msa1061900:CDS
GGAAGGAACACCATTTGCAG+TGG	0.632915	7_2:-52355583	None:intergenic
GGGACATCAAACGAAGTCGT+TGG	0.635975	7_2:+52355807	Msa1061900:CDS
ACATTGTTAAACTCAAACCC+GGG	0.639732	7_2:+52355787	Msa1061900:CDS
ACTATTCCATTGCTGGCCCG+GGG	0.640157	7_2:+52355686	Msa1061900:CDS
ATCCACACATCATTCTATTG+GGG	0.654547	7_2:+52355648	Msa1061900:CDS
AAACCATAAACCCAATAACA+TGG	0.667055	7_2:-52355938	None:intergenic
GACCCCAATAGAATGATGTG+TGG	0.683603	7_2:-52355650	None:intergenic
GCCCAACATCGTCGCCACCG+TGG	0.693359	7_2:-52355610	None:intergenic
CCAAGTACGAGAATCCAGTA+CGG	0.693631	7_2:-52355860	None:intergenic
ACACCGACATCACAAACACG+AGG	0.739798	7_2:-52355740	None:intergenic
TGTTCCTTCCAACTCCACGG+TGG	0.776714	7_2:+52355596	Msa1061900:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACATTGTTAAACTCAAACC+CGG	+	chr7_2:52355786-52355805	Msa1061900:CDS	30.0%
	AAACCATAAACCCAATAACA+TGG	-	chr7_2:52355941-52355960	None:intergenic	30.0%
!	GTTATTGGGTTTATGGTTTT+TGG	+	chr7_2:52355942-52355961	Msa1061900:CDS	30.0%
!!	ATGGCTATTTTGAAGAAATC+TGG	+	chr7_2:52355969-52355988	Msa1061900:CDS	30.0%
!!	CTATTTTGAAGAAATCTGGT+GGG	+	chr7_2:52355973-52355992	Msa1061900:CDS	30.0%
	CAATAGAATGATGTGTGGAT+CGG	-	chr7_2:52355648-52355667	None:intergenic	35.0%
	GATCCACACATCATTCTATT+GGG	+	chr7_2:52355647-52355666	Msa1061900:CDS	35.0%
	ATCCACACATCATTCTATTG+GGG	+	chr7_2:52355648-52355667	Msa1061900:CDS	35.0%
	TAGACATACTATTCCATTGC+TGG	+	chr7_2:52355679-52355698	Msa1061900:CDS	35.0%
!	GGGTTTGAGTTTAACAATGT+TGG	-	chr7_2:52355787-52355806	None:intergenic	35.0%
	ACATTGTTAAACTCAAACCC+GGG	+	chr7_2:52355787-52355806	Msa1061900:CDS	35.0%
	ATTCTCGTACTTGGTTATGT+TGG	+	chr7_2:52355869-52355888	Msa1061900:CDS	35.0%
!	TTGGCTGTTATGTCGTTTAA+TGG	+	chr7_2:52355888-52355907	Msa1061900:CDS	35.0%
!!!	GTTTATGGTTTTTGGTACGA+TGG	+	chr7_2:52355950-52355969	Msa1061900:CDS	35.0%
!!	GCTATTTTGAAGAAATCTGG+TGG	+	chr7_2:52355972-52355991	Msa1061900:CDS	35.0%
	CGATCCACACATCATTCTAT+TGG	+	chr7_2:52355646-52355665	Msa1061900:CDS	40.0%
!!!	AAGTCGTTGGCTCACTTTTA+AGG	+	chr7_2:52355820-52355839	Msa1061900:CDS	40.0%
!	GCTGTTATGTCGTTTAATGG+TGG	+	chr7_2:52355891-52355910	Msa1061900:CDS	40.0%
	TGGCCATGTTATTGGGTTTA+TGG	+	chr7_2:52355935-52355954	Msa1061900:CDS	40.0%
	TTGTTCACCACCACTGCAAA+TGG	+	chr7_2:52355573-52355592	Msa1061900:CDS	45.0%
	GACCCCAATAGAATGATGTG+TGG	-	chr7_2:52355653-52355672	None:intergenic	45.0%
	ATACTATTCCATTGCTGGCC+CGG	+	chr7_2:52355684-52355703	Msa1061900:CDS	45.0%
	GATCCTCGTGTTTGTGATGT+CGG	+	chr7_2:52355737-52355756	Msa1061900:CDS	45.0%
	AACGACTTCGTTTGATGTCC+CGG	-	chr7_2:52355808-52355827	None:intergenic	45.0%
!!!	CGTTGGCTCACTTTTAAGGA+CGG	+	chr7_2:52355824-52355843	Msa1061900:CDS	45.0%
!!	GTTGGCTCACTTTTAAGGAC+GGG	+	chr7_2:52355825-52355844	Msa1061900:CDS	45.0%
!!	TTGGCTCACTTTTAAGGACG+GGG	+	chr7_2:52355826-52355845	Msa1061900:CDS	45.0%
	CCAAGTACGAGAATCCAGTA+CGG	-	chr7_2:52355863-52355882	None:intergenic	45.0%
!!	CAGTTGGTGGCCATGTTATT+GGG	+	chr7_2:52355928-52355947	Msa1061900:CDS	45.0%
	AGGAACACCATTTGCAGTGG+TGG	-	chr7_2:52355583-52355602	None:intergenic	50.0%
	GGAAGGAACACCATTTGCAG+TGG	-	chr7_2:52355586-52355605	None:intergenic	50.0%
	TGGTGTTCCTTCCAACTCCA+CGG	+	chr7_2:52355593-52355612	Msa1061900:CDS	50.0%
	TGTGGATCGGAATTCTTCGC+CGG	-	chr7_2:52355635-52355654	None:intergenic	50.0%
	TACTATTCCATTGCTGGCCC+GGG	+	chr7_2:52355685-52355704	Msa1061900:CDS	50.0%
	ACACCGACATCACAAACACG+AGG	-	chr7_2:52355743-52355762	None:intergenic	50.0%
	CGTGTTTGTGATGTCGGTGT+TGG	+	chr7_2:52355743-52355762	Msa1061900:CDS	50.0%
!!	GTTTGTGATGTCGGTGTTGG+TGG	+	chr7_2:52355746-52355765	Msa1061900:CDS	50.0%
	ACGACTTCGTTTGATGTCCC+GGG	-	chr7_2:52355807-52355826	None:intergenic	50.0%
	GGGACATCAAACGAAGTCGT+TGG	+	chr7_2:52355807-52355826	Msa1061900:CDS	50.0%
!!	CTTTTAAGGACGGGGCTGTA+CGG	+	chr7_2:52355834-52355853	Msa1061900:CDS	50.0%
	CCGTACTGGATTCTCGTACT+TGG	+	chr7_2:52355860-52355879	Msa1061900:CDS	50.0%
	GGTGTGTTTCTTGCTGCAGT+TGG	+	chr7_2:52355912-52355931	Msa1061900:CDS	50.0%
	GTGTTTCTTGCTGCAGTTGG+TGG	+	chr7_2:52355915-52355934	Msa1061900:CDS	50.0%
!!	GCAGTTGGTGGCCATGTTAT+TGG	+	chr7_2:52355927-52355946	Msa1061900:CDS	50.0%
	TGTTCCTTCCAACTCCACGG+TGG	+	chr7_2:52355596-52355615	Msa1061900:CDS	55.0%
	ACTATTCCATTGCTGGCCCG+GGG	+	chr7_2:52355686-52355705	Msa1061900:CDS	55.0%
!!	TGATGTCGGTGTTGGTGGAG+TGG	+	chr7_2:52355751-52355770	Msa1061900:CDS	55.0%
	TCCACGGTGGCGACGATGTT+GGG	+	chr7_2:52355609-52355628	Msa1061900:CDS	60.0%
	TACATGACAGCGCTGTCCCC+GGG	-	chr7_2:52355705-52355724	None:intergenic	60.0%
	GTACATGACAGCGCTGTCCC+CGG	-	chr7_2:52355706-52355725	None:intergenic	60.0%
	GTCGCCACCGTGGAGTTGGA+AGG	-	chr7_2:52355603-52355622	None:intergenic	65.0%
	CATCGTCGCCACCGTGGAGT+TGG	-	chr7_2:52355607-52355626	None:intergenic	65.0%
	CTCCACGGTGGCGACGATGT+TGG	+	chr7_2:52355608-52355627	Msa1061900:CDS	65.0%
	GGGCTGTACGGAGTCCGTAC+TGG	+	chr7_2:52355846-52355865	Msa1061900:CDS	65.0%
	GCCCAACATCGTCGCCACCG+TGG	-	chr7_2:52355613-52355632	None:intergenic	70.0%
	CGGTGGCGACGATGTTGGGC+CGG	+	chr7_2:52355613-52355632	Msa1061900:CDS	70.0%
	CGCTGTCCCCGGGCCAGCAA+TGG	-	chr7_2:52355695-52355714	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	52355570	52356019	52355570	ID=Msa1061900;Name=Msa1061900
chr7_2	mRNA	52355570	52356019	52355570	ID=Msa1061900-mRNA-1;Parent=Msa1061900;Name=Msa1061900-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|149
chr7_2	exon	52355570	52356019	52355570	ID=Msa1061900-mRNA-1:exon:9454;Parent=Msa1061900-mRNA-1
chr7_2	CDS	52355570	52356019	52355570	ID=Msa1061900-mRNA-1:cds;Parent=Msa1061900-mRNA-1

Gene Sequence

>Msa1061900

ATGTTGTTCACCACCACTGCAAATGGTGTTCCTTCCAACTCCACGGTGGCGACGATGTTGGGCCGGCGAAGAATTCCGATCCACACATCATTCTATTGGGGTCATAATGTAGACATACTATTCCATTGCTGGCCCGGGGACAGCGCTGTCATGTACGCAGTAGCGCTGATCCTCGTGTTTGTGATGTCGGTGTTGGTGGAGTGGCTTTCATTTACCAACATTGTTAAACTCAAACCCGGGACATCAAACGAAGTCGTTGGCTCACTTTTAAGGACGGGGCTGTACGGAGTCCGTACTGGATTCTCGTACTTGGTTATGTTGGCTGTTATGTCGTTTAATGGTGGTGTGTTTCTTGCTGCAGTTGGTGGCCATGTTATTGGGTTTATGGTTTTTGGTACGATGGCTATTTTGAAGAAATCTGGTGGGTTAGATTCTTCTTCTAAACCATAG

Protein sequence

>Msa1061900

MLFTTTANGVPSNSTVATMLGRRRIPIHTSFYWGHNVDILFHCWPGDSAVMYAVALILVFVMSVLVEWLSFTNIVKLKPGTSNEVVGSLLRTGLYGVRTGFSYLVMLAVMSFNGGVFLAAVGGHVIGFMVFGTMAILKKSGGLDSSSKP*